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- Forsberg, M, et al.
(författare)
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Efficient reprogramming of adult neural stem cells to monocytes by ectopic expression of a single gene
- 2010
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Ingår i: Proceedings of the National Academy of Sciences of the United States of America. - : Proceedings of the National Academy of Sciences. - 1091-6490. ; 107:33, s. 14657-14661
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Tidskriftsartikel (refereegranskat)abstract
- Neural stem cells have a broad differentiation repertoire during embryonic development and can be reprogrammed to pluripotency comparatively easily. We report that adult neural stem cells can be reprogrammed at very high efficiency to monocytes, a differentiated fate of an unrelated somatic lineage, by ectopic expression of the Ets transcription factor PU.1. The reprogrammed cells display a marker profile and functional characteristics of monocytes and integrate into tissues after transplantation. The failure to reprogram lineage-committed neural cells to monocytes with PU.1 suggests that neural stem cells are uniquely amenable to reprogramming.
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| 10. |
- Carlen, M, et al.
(författare)
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Genetic visualization of neurogenesis
- 2006
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Ingår i: Experimental cell research. - : Elsevier BV. - 0014-4827. ; 312:15, s. 2851-2859
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Tidskriftsartikel (refereegranskat)
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| 11. |
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| 12. |
- Hansson, EM, et al.
(författare)
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Recording Notch signaling in real time
- 2006
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Ingår i: Developmental neuroscience. - : S. Karger AG. - 0378-5866 .- 1421-9859. ; 28:1-2, s. 118-127
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Tidskriftsartikel (refereegranskat)abstract
- Notch signaling is a highly conserved signaling pathway, which is critical for many cell fate decisions. Ligand activation of Notch leads to cleavage of the Notch receptor and liberation of the Notch intracellular domain (ICD) from the membrane-tethered receptor. After translocation to the nucleus, the Notch ICD interacts with the DNA-binding protein CSL to activate gene transcription. To better understand the temporal and spatial aspects of Notch signaling, we here describe a fluorescent protein-based reporter assay that allows Notch activation to be followed in real time in individual cells. We have generated a reporter construct composed of 12 CSL-binding motifs linked to fluorescent proteins with different half-lives: a stabler red fluorescent protein (DsRedExpressDR) and a destabilized form of green fluorescent protein (d1EGFP). The fluorescent reporters reflect the activation status of Notch signaling with single-cell resolution. The reporters rapidly respond to various forms of Notch activation, including ligand activation of full-length Notch receptors. Finally, we use this assay to gain insights into the level of Notch signaling in CNS progenitor cells in culture and in vivo.
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| 13. |
- Lazaridis, I, et al.
(författare)
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A hypothalamus-habenula circuit controls aversion
- 2019
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Ingår i: Molecular psychiatry. - : Springer Science and Business Media LLC. - 1476-5578 .- 1359-4184. ; 24:9, s. 1351-1368
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Tidskriftsartikel (refereegranskat)
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| 14. |
- Lu, S, et al.
(författare)
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Assessing the replicability of spatial gene expression using atlas data from the adult mouse brain
- 2021
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Ingår i: PLoS biology. - : Public Library of Science (PLoS). - 1545-7885. ; 19:7, s. e3001341-
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Tidskriftsartikel (refereegranskat)abstract
- High-throughput, spatially resolved gene expression techniques are poised to be transformative across biology by overcoming a central limitation in single-cell biology: the lack of information on relationships that organize the cells into the functional groupings characteristic of tissues in complex multicellular organisms. Spatial expression is particularly interesting in the mammalian brain, which has a highly defined structure, strong spatial constraint in its organization, and detailed multimodal phenotypes for cells and ensembles of cells that can be linked to mesoscale properties such as projection patterns, and from there, to circuits generating behavior. However, as with any type of expression data, cross-dataset benchmarking of spatial data is a crucial first step. Here, we assess the replicability, with reference to canonical brain subdivisions, between the Allen Institute’s in situ hybridization data from the adult mouse brain (Allen Brain Atlas (ABA)) and a similar dataset collected using spatial transcriptomics (ST). With the advent of tractable spatial techniques, for the first time, we are able to benchmark the Allen Institute’s whole-brain, whole-transcriptome spatial expression dataset with a second independent dataset that similarly spans the whole brain and transcriptome. We use regularized linear regression (LASSO), linear regression, and correlation-based feature selection in a supervised learning framework to classify expression samples relative to their assayed location. We show that Allen Reference Atlas labels are classifiable using transcription in both data sets, but that performance is higher in the ABA than in ST. Furthermore, models trained in one dataset and tested in the opposite dataset do not reproduce classification performance bidirectionally. While an identifying expression profile can be found for a given brain area, it does not generalize to the opposite dataset. In general, we found that canonical brain area labels are classifiable in gene expression space within dataset and that our observed performance is not merely reflecting physical distance in the brain. However, we also show that cross-platform classification is not robust. Emerging spatial datasets from the mouse brain will allow further characterization of cross-dataset replicability ultimately providing a valuable reference set for understanding the cell biology of the brain.
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| 17. |
- Meletis, K, et al.
(författare)
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Have the bloody cells gone to our heads?
- 2001
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Ingår i: The Journal of cell biology. - : Rockefeller University Press. - 0021-9525 .- 1540-8140. ; 155:5, s. 699-702
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Tidskriftsartikel (refereegranskat)abstract
- Recent studies have shown that cells expressing neuronal antigens can be derived from a bone marrow transplant. A new report lends support to and extends these previous results by presenting compelling morphological evidence for the generation and integration of highly differentiated bone marrow–derived neurons.
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| 19. |
- Pappas, S. D., et al.
(författare)
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Growth and Experimental Evidence of Quantum Confinement Effects in Cu(2)O and CuO Thin Films
- 2011
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Ingår i: Journal of Nano Research. - 1662-5250. ; 15, s. 69-74
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Tidskriftsartikel (refereegranskat)abstract
- Thin Cu films of thickness 0.4 - 150 nm were deposited via radio frequency magnetron sputtering on Si(100) wafers, corning glass and quartz. Subsequently the Cu films were oxidized in ambient air at 230 degrees C and 425 degrees C in order to produce single-phase Cu(2)O and CuO, respectively. Selected samples were measured in the transmission geometry with the help of an ultraviolet - visible spectrophotometer. From the absorption spectra of the films, it was found that the gap E(B) for the dipole allowed transitions showed blue shifts of about 1.2 eV for the Cu(2)O thinnest film (0.75 nm), whereas the E(direct) for the direct gap transitions showed blue shifts of about 0.16 eV for the CuO thinnest film (0.7 mm). The blue shift of the energy gap in the copper-oxide semiconductors is an indication of the presence of strong quantum confinement effects.
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| 20. |
- Poulopoulos, P., et al.
(författare)
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Growth and Magnetism of Natural Multilayers
- 2011
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Ingår i: Journal of Nano Research. - 1662-5250. ; 15, s. 95-103
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Tidskriftsartikel (refereegranskat)abstract
- In this work, we present a simple method to fabricate high quality Ni/NiO multilayers with the use of a single magnetron sputtering head. Namely, at the end of the deposition of each single Ni layer, air is let to flow into the vacuum chamber through a leak valve. Then, a very thin NiO layer (similar to 1nm) is formed by natural oxidation. The process is reproducible and the result is the formation of a multilayer with excellent layering. Magnetization hysteresis loops recorded at 5 K and room temperature reveal a tendency for perpendicular magnetic anisotropy as the thickness of the individual Ni layers decreases. It is shown that the Ni/NiO interface has sizeable positive surface/interface anisotropy, i.e. it favors the development of perpendicular magnetic anisotropy. This is rather unusual for a Ni-based multilayered system and may render Ni/NiO multilayers useful for magneto-optical recording applications.
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| 24. |
- Wu, HH, et al.
(författare)
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Distinct subtypes of proprioceptive dorsal root ganglion neurons regulate adaptive proprioception in mice
- 2021
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Ingår i: Nature communications. - : Springer Science and Business Media LLC. - 2041-1723. ; 12:1, s. 1026-
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Tidskriftsartikel (refereegranskat)abstract
- Proprioceptive neurons (PNs) are essential for the proper execution of all our movements by providing muscle sensory feedback to the central motor network. Here, using deep single cell RNAseq of adult PNs coupled with virus and genetic tracings, we molecularly identify three main types of PNs (Ia, Ib and II) and find that they segregate into eight distinct subgroups. Our data unveil a highly sophisticated organization of PNs into discrete sensory input channels with distinct spatial distribution, innervation patterns and molecular profiles. Altogether, these features contribute to finely regulate proprioception during complex motor behavior. Moreover, while Ib- and II-PN subtypes are specified around birth, Ia-PN subtypes diversify later in life along with increased motor activity. We also show Ia-PNs plasticity following exercise training, suggesting Ia-PNs are important players in adaptive proprioceptive function in adult mice.
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