| 1. |
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| 2. |
- Arakelian, Erebouni, 1973-, et al.
(författare)
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How Human Resources Index, Relational Justice, and Perceived Productivity Change after Reorganization at a Hospital in Sweden That Uses a Structured Support Model for Systematic Work Environment Management
- 2021
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Ingår i: International Journal of Environmental Research and Public Health. - : MDPI. - 1661-7827 .- 1660-4601. ; 18:21
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Tidskriftsartikel (refereegranskat)abstract
- To facilitate systematic work environment management, which should be a natural part of business development, a structured support model was developed. The Stamina model has previously been used in Swedish municipalities, showing positive results. The aim was to study how the Human Resources Index (HRI), relational justice, short-term recovery and perceived productivity changed in a recently reorganised perioperative setting in a hospital in Sweden that uses a structured support model for systematic work environment management. A longitudinal design that took measurements at four time points was used in a sample of 500 employees in a perioperative hospital department. The results for the overall sample indicated a positive trend in the HRI (Mt1 = 48.5, SDt1 = 22.5; Mt3 = 56.7, SDt1 = 21.2; p < 0.001). Perceived health-related production loss (Mdt1 = 2, IQR = 3; Mdt3 = 0, IQR = 3; p < 0.001) and perceived work environment-related production loss (Mdt1 = 2, IQR = 3; Mdt3 = 0, IQR = 4; p < 0.001) showed major improvements. Short-term recovery showed a minor improvement (Mt1 = 2.61, SDt1 = 1.33; Mt3 = 2.65, SDt3 = 1.22; p = 0.872). In conclusion, the implementation of the Stamina model, of which the HRI constitutes an important part, seems to be a helpful tool to follow-up on work environment processes, and minimise production losses due to health and work environment-related issues.
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| 3. |
- Arakelian, Erebouni, 1973-, et al.
(författare)
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Success factors when implementing a structured support model for systematic work environment management in operating departments : A case study from Sweden
- 2022
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Ingår i: Journal of Nursing Management. - : John Wiley & Sons. - 0966-0429 .- 1365-2834. ; 30:7, s. 3618-3627
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Tidskriftsartikel (refereegranskat)abstract
- Aim The study aimed to investigate how departments in a Swedish hospital worked with a structured support model between the sessions and what they identified as success factors. Background To improve the work environment in a Swedish hospital, a structured support model for systematic work environment management was implemented in operating departments. The structured work starts with sending a web-based, open-ended, anonymous questionnaire to all employees. In response, employees describe how they perceive their work environment 'right now'. Next, a session is held where employees' viewpoints are discussed, and areas of improvement are agreed upon. Action plans are created between the sessions, and the employees start working with their plans with support from their managers. Implementing new models takes time and requires efforts from employees and managers. Method A case study was conducted, including three operating departments within a perioperative organization in a university hospital in Sweden. The participating departments had used the model without interruption during the Covid-19 pandemic 2 years after implementation, and they had created a customized working method. Three first-line managers were interviewed, and 22 action plans, 21 workplace meeting notes and two presentations were analysed using thematic analysis. Results The results are sorted under three main thematic headings: Experience of results and benefits, Marketing and cheering on and Making adjustments and making the model one's own. The results from the action plans and workplace meetings indicated that the employees had discussed problems with cooperation, work organization and how to treat each other. Conclusion Human factors, such as support, encouragement, seeing the benefits, allowing for time and respecting each other can facilitate and contribute to the implementation and success of a new model. Implications for Nursing Management The main finding of the study indicates that with a structured way of working, and with the participation of the employees in the systematic work environment work, the employees contributed with constructive suggestions for improvement. This, in turn, contributed to reducing the workload for first-line managers. In addition, when working with a structured model, deficiencies in the workplace were identified, which triggered an improvement process in the participating hospital departments.
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| 4. |
- Bengtsson-Palme, Johan, 1985, et al.
(författare)
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Metagenomics reveals that detoxification systems are underrepresented in marine bacterial communities
- 2014
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Ingår i: BMC Genomics. - : Springer Science and Business Media LLC. - 1471-2164. ; 15:749
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Tidskriftsartikel (refereegranskat)abstract
- Background Environmental shotgun sequencing (metagenomics) provides a new way to study communities in microbial ecology. We here use sequence data from the Global Ocean Sampling (GOS) expedition to investigate toxicant selection pressures revealed by the presence of detoxification genes in marine bacteria. To capture a broad range of potential toxicants we selected detoxification protein families representing systems protecting microorganisms from a variety of stressors, such as metals, organic compounds, antibiotics and oxygen radicals. Results Using a bioinformatics procedure based on comparative analysis to finished bacterial genomes we found that the amount of detoxification genes present in marine microorganisms seems surprisingly small. The underrepresentation is particularly evident for toxicant transporters and proteins involved in detoxifying metals. Exceptions are enzymes involved in oxidative stress defense where peroxidase enzymes are more abundant in marine bacteria compared to bacteria in general. In contrast, catalases are almost completely absent from the open ocean environment, suggesting that peroxidases and peroxiredoxins constitute a core line of defense against reactive oxygen species (ROS) in the marine milieu. Conclusions We found no indication that detoxification systems would be generally more abundant close to the coast compared to the open ocean. On the contrary, for several of the protein families that displayed a significant geographical distribution, like peroxidase, penicillin binding transpeptidase and divalent ion transport protein, the open ocean samples showed the highest abundance. Along the same lines, the abundance of most detoxification proteins did not increase with estimated pollution. The low level of detoxification systems in marine bacteria indicate that the majority of marine bacteria have a low capacity to adapt to increased pollution. Our study exemplifies the use of metagenomics data in ecotoxicology, and in particular how anthropogenic consequences on life in the sea can be examined.
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| 5. |
- Bergström Lind, Sara, et al.
(författare)
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Immunoaffinity Enrichments Followed by Mass Spectrometric Detection for Studying Global Protein Tyrosine Phosphorylation
- 2008
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Ingår i: Journal of Proteome Research. - : American Chemical Society (ACS). - 1535-3893 .- 1535-3907. ; 7:7, s. 2897-2910
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Tidskriftsartikel (refereegranskat)abstract
- Phosphorylation of protein tyrosine residues regulates important cell functions and is, when dysregulated, often crucially involved in oncogenesis. It is therefore important to develop and evaluate methods for identifying and studying tyrosine phosphorylated (P-Tyr) proteins. P-Tyr proteins are present at very low concentrations within cells, requiring highly selective enrichment methods to be detected. In this study, we applied immunoaffinity as enrichment step for P-Tyr proteins. Five selected anti-phosphotyrosine antibodies (monoclonal antibodies 4G10, PY100, PYKD1, 13F9 and one polyclonal antiserum) were evaluated with respect to their capability to enrich P-Tyr proteins from cell extracts of the K562 leukemia cell line. The enrichment resulted in the detection of a group of proteins that potentially were tyrosine-phosphorylated (putative P-Tyr proteins). High accuracy identification of actual P-Tyr sites were performed using a highly selective and sensitive liquid chromatography Fourier transform mass spectrometer (LC-FTMS) setup with complementary collision activated dissociation (CAD) and electron capture dissociation (ECD) fragmentations. 4G10 and PY100 antibodies recognized the greatest number of putative P-Tyr proteins in initial screening experiments and were therefore further evaluated and compared in immunoaffinity enrichment of both P-Tyr proteins and peptides. Using the 4G10 antibody for enrichment of proteins, we identified 459 putative P-Tyr proteins by MS. Out of these proteins, 12 were directly verified as P-Tyr proteins by MS analysis of the actual site. Using the PY100 antibody for enrichment of peptides, we detected 67 P-Tyr peptides (sites) and 89 putative P-Tyr proteins. Generally, enrichment at the peptide level made it difficult to reliably determine the identity of the proteins. In contrast, protein identification following immunoaffinity enrichment at the protein level gave greater sequence coverage and thus a higher confidence in the protein identification. By combining all available information, 40 proteins were identified as true P-Tyr proteins from the K562 cell line. In conclusion, this study showed that a combination of immunoaffinity enrichment using multiple antibodies of both intact and digested proteins in parallel experiments is required for best possible coverage of all possible P-Tyr proteins in a sample.
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| 6. |
- Edholm, Dan, et al.
(författare)
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Adenovirus vector designed for expression of toxic proteins
- 2001
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Ingår i: Journal of Virology. - 0022-538X .- 1098-5514. ; 75:20, s. 9579-9584
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Tidskriftsartikel (refereegranskat)abstract
- To construct recombinant adenoviruses expressing biologically active proteins may be impossible, or result in a significant reduction in virus yield, if the protein expressed has an inhibitory effect on virus replication or cellular growth. To overcome this problem, we previously designed adenovirus vectors expressing foreign proteins from inducible promoters. However, during our work with a replication-deficient virus expressing the ASF/SF2 splicing factor from a progesterone antagonist-inducible gene cassette, we discovered that ASF/SF2 was expressed at a significant level in the 293 producer cell line, even in the absence of inducer. 293 cells code for adenovirus E1A and E1B proteins and thus support the growth of E1-deficient adenoviruses. Here we show that this background ASF/SF2 expression results from a low level of E1A-mediated transactivation of the basal promoter driving transgene expression. To overcome the problem of leaky expression, we reconstructed a novel gene cassette that combines an inducible promoter and a Lac repressor protein-based block to reduce transcriptional elongation. We show that this novel vector system dramatically reduced background transgene expression and therefore should be useful for the rescue and propagation of high-titer stocks of recombinant adenoviruses expressing toxic proteins.
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| 7. |
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| 8. |
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| 9. |
- Gidlöf, Olof, et al.
(författare)
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Complete discrimination of six individuals based on high-resolution melting of hypervariable regions I and II of the mitochondrial genome
- 2009
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Ingår i: BioTechniques. - : Future Science Ltd. - 0736-6205 .- 1940-9818. ; 47:2, s. 671-678
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Tidskriftsartikel (refereegranskat)abstract
- Analysis of mitochondrial DNA in forensic samples is routinely carried out by direct sequencing of hypervariable regions within the non-coding displacement loop. Although the accuracy and sensitivity of this method cannot be questioned, it is both time-consuming and labor intensive. Finding a way to rapidly pre-screen forensic samples-prior to sequencing, to reduce the number of samples that need to be sequenced-would greatly benefit forensic laboratories. Herein, we describe an assay for discrimination of DNA from different individuals based on high-resolution melting analysis of the two hypervariable regions HVI and HVII of the mitochondrial genome. By clearly distinguishing the DNA melting curves of six different individuals, we show that this assay has the potential to function as a rapid and inexpensive pre-screening method for forensic samples prior to DNA sequencing.
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| 10. |
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| 11. |
- Hellman, Therese, et al.
(författare)
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A Mixed-Method Study of Providing and Implementing a Support Model Focusing on Systematic Work Environment Management
- 2020
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Ingår i: Journal of Occupational and Environmental Medicine. - : LIPPINCOTT WILLIAMS & WILKINS. - 1076-2752 .- 1536-5948. ; 62:4, s. E160-E166
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Tidskriftsartikel (refereegranskat)abstract
- Aims: To explore how a support model (Stamina) targeting the systematic work environment management was experienced, provided and implemented in Swedish municipalities. Methods: A mixed method approach was applied, including material from interviews and questionnaire. The questionnaire was answered by 123 persons (67.5%), and 18 participants were interviewed twice during a one year period. Data were analysed using descriptive statistics and qualitative content analysis. Results: The participants followed the structure of the Stamina model in their practical work. Still, there was variation in the practical provision of the model. The implementation was supported by their organizations and was influenced by various actors. Creating commitment was an essential part to facilitate implementation. Conclusion: To promote implementation of the Stamina model, there is a need to create commitment among stakeholders that will participate in the implementation.
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| 12. |
- Hellman, Therese, et al.
(författare)
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A Qualitative Study on Employees' Experiences of a Support model for Systematic Work Environment Management
- 2019
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Ingår i: International Journal of Environmental Research and Public Health. - : MDPI. - 1661-7827 .- 1660-4601. ; 16:19
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Tidskriftsartikel (refereegranskat)abstract
- Background: The aim is to explore how an organisational work environment support model, the Stamina model, influences employees' work situations and the development of sustainable work systems. Methods: It was a qualitative study with semi-structured, focus-group interviews, including 45 employees from six work groups. Eighteen focus group interviews were conducted over a period of two years. Data were analysed with constant comparative method. Results: The core category, shifting focus from an individual to an organisational perspective of work, illustrated how communication and increased understanding of one's work tasks changed over time and contributed to deeper focus on the actual operation. These insights were implemented at different time points among the work groups during the two-year process. Conclusions: Our results indicate that working with the model engages employees in the work environment management, puts emphasis on reflections and discussions about the meaning and purpose of the operations and enables a shared platform for communication. These are important features that need to continue over time in order to create a sustainable work system. The Stamina model, thus seems to have the potential to promote productive and healthy work places.
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| 13. |
- Hellman, Therese, et al.
(författare)
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How management groups' reason when deciding to use a model focusing on systematic work environment management : Experiences from Swedish municipalities
- 2019
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Ingår i: International Journal of Workplace Health Management. - : EMERALD GROUP PUBLISHING LTD. - 1753-8351 .- 1753-836X. ; 12:6, s. 441-456
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Tidskriftsartikel (refereegranskat)abstract
- Purpose The purpose of this paper is to explore and describe the perspective of the management group regarding how they reasoned when deciding to engage in a model focussing on systematic work environment management, and what motives that influenced their decision. Design/methodology/approach This qualitative study with semi-structured interviews includes 18 representatives from the management groups in 18 Swedish municipalities. Data were analysed with a constant comparative method. Findings The participants described two aspects that were of importance when making the decision; establishing commitment before making the decision and establishing strategies to legitimise the decision. Furthermore, they expressed motives that were linked both to their individual expectations and wishes and to policies and facts in their organisations. The participants experienced the model as a valuable tool in their organisations to increase employee participation and to provide structured support to their first-line managers. Practical implications - The managers' motives were linked to individual expectations and external directives. These were often intertwined and influenced their decisions. When implementing this type of model, it is important to discuss decisions in a larger group to avoid building an organisational initiative on one person's expectations. Furthermore, it is important to support the management's work to establish commitment for the model in the municipal organisation. Originality/value This study adds to knowledge of the complexity of deciding and implementing models to support systematic work environment management in organisations.
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| 14. |
- Hjort, Magnus, 1965-
(författare)
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"Nationens livsfråga" : Propaganda och upplysning i försvarets tjänst 1944-1963
- 2004
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Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- The subject of this thesis is the problem of autonomy versus control in the field of defence propaganda and defence information. The study has its foundation in two classical problems of democratic theory: the issue of political control over bureaucracies and the question of the involvement of non-governmental organizations in public administration. I argue that these two problems of modern democracy can be seen as one, the problem of autonomy versus control. In my thesis I analyse this problem within the framework of the tension between the armed forces, the government and the non-governmental organizations in the field of military information between 1944 and 1963. In the book I use the terms defence information and defence propaganda synonymously, meaning information distributed with the intent to influence attitudes and actions of individuals and/or organizations. This book focuses on four aspects of the problem of autonomy versus control. The first aspect deals with propaganda coming from the supreme commander and the defence staff. What methods were used? With which other groups did they collaborate? Can we see any change over the course of time? Aspect number two focuses on government control over defence information. What means of control did the government use? When was it used and how? The third aspect concerns non-governmental organizations in the field of defence. How did they interact with the government and with the defence staff and the supreme commander? The final aspect deals with government control over non-governmental organizations as well as control exercised by the military authorities over non-governmental organizations. What means of control was employed, at what times, and how? The analysis of these aspects is undertaken in five case studies.The study shows that the problem of autonomy versus control was evident from 1944 to 1963. I also argue that the supreme commander and the defence staff acted as a military interest group with close ties to the conservative daily newspaper Svenska Dagbladet, to business elites and to the Conservative Party. In the 1940’s and 1950’s the government used various informal methods to try to control the military propaganda. In 1960 this changed and the government turned to more formal ways of control such as introducing new regulations concerning military information.
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| 15. |
- Jani, Yahya, 1975-, et al.
(författare)
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VA-kluster Mälardalen : Kunskapssammanställning om klustrets forskningsområden
- 2023
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Rapport (refereegranskat)abstract
- VA-kluster Mälardalen bildades år 2010 med syftet att samla regional forskningskompetensoch verksamhetsutövare inom VA-organisationer kring ett samarbete rörandereningstekniker, uppströmsarbete, resursåterföring och digitalisering inom VA-sektorn.Klustret utgörs idag av fem universitet, tretton VA-organisationer och två forskningsinstitut,som tillsammans forskar för en hållbar och resurseffektiv VA-sektor. Klustrets har drivitsamarbetet mellan VA-organisationer och lärosäten/institut på ett sätt som ger VAorganisationerna möjlighet att prioritera och initiera samarbetsprojekt inriktad på FoU samtrelevanta forskningsfrågor kopplade till VA-organisationers dagliga aktiviteter och verkligaproblem.Klustret har varit en viktig plattform för att såväl kommunicera och informeraforskningsresultat internt inom klustret som nationellt och internationellt inom de treprioriterade forskningsområdena: i) System och reningstekniker med närings- ochresursåterföring; ii) Metodik, teknik och kunskap för uppströmsarbete och hållbara kretslopp;och iii) Digitala tekniker för resurseffektiva avloppssystem. VA-kluster Mälardalen har totaltgenomfört cirka 100 forsknings- och utvecklingsprojekt fram tills nu. Många av projektenhar dessutom genomförts i samarbete med andra VA-organisationer utanför klustret samtmed internationella samarbetspartners. VA-kluster Mälardalen har bidragit med ökadkunskap och utveckling av framtida hållbara reningsverk i Sverige, EU och övriga världen.
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| 16. |
- Karresand, Helena, 1969-, et al.
(författare)
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How passive are your activities? : An interdisciplinary comparative energy analysis of passive and conventional houses in Linköping
- 2009
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Rapport (övrigt vetenskapligt/konstnärligt)abstract
- Vilken påverkan har vårt vardagsliv och de aktiviteter som sker i hemmet på vår miljö lokalt och globalt sett? Hur bidrar vi till den nu så aktuella frågan om global uppvärmning? Vanliga företeelser såsom matlagning, dörrar och fönster som öppnas, att ta ett bad eller en sådan grundläggande sak som vår närvaro är alla exempel som påverkar det globala energisystemet. Om en dörr öppnas eller om en ugn sätts på påverkas inomhusklimatet genom förändringar i inomhustemperatur och luftkvalité. Som en följd av detta påverkas även energianvändningen i huset vi bor i samt det lokala energisystemet vi befinner oss i. Förändringar i lokala energisystem får i sin tur följder för det globala energisystemet och därmed klimatet på global nivå. Därmed är det inte sagt att allt vi i vardagen gör indirekt har negativa konsekvenser på det globala klimatet. De två följande frågorna utgör grunden till denna rapport: Vad kan göras för att minska de negativa effekterna av en energipåverkande aktivitet? Och vad finns det för möjligheter att göra detta? Ett alternativ är att förbättra våra byggnader så att den värme som kommer från solen, våra kroppar och aktiviteter tillvaratas på ett optimalt sätt, just detta är grundtanken med konceptet passivhus som under det senaste årtiondet fått alltmer gehör. I ett passivhus använder man sig av ett välisolerat klimatskal samt värmeväxling för att uppnå ett behagligt inomhusklimat samtidigt som en minimering av uppvärmningsbehovet sker. Denna studie omfattar ett antal nybyggda passivhus och ett antal konventionella hus i bostadsområdet Lambohov i Linköping. Här undersöks hushållsaktiviteters påverkan på energibalansen i båda hustyperna samt vilka effekter en omfattande ombyggnation till passivhus kan ha på Linköpings energisystem. Studien jämför hur uppvärmningsystemet påverkar inomhusklimatet för de boende. I studien ingår även en undersökning om de förväntningar de nyinflyttade har på passivhusen samt de förväntningar som bostadsbolaget har på hyresgästerna. Vidare undersöks de faktorer som motiverade byggandet utifrån bostadsbolagets samt staden Linköpings perspektiv. Denna rapport går bakom kulisserna på passivhusen i Lambohov och söker information kring aktörer och hyresgäster av dessa passiva radhus-hyreslägenheter. En bottom-up-metod används för att få en realistisk bild av hushållets verksamheter som bidrar till den passiva uppvärmningen av byggnaden. Mätningar av energianvändning och termiskt inomhusklimat sker i precisionen 5-sekunder och extrapoleras i både tid och rum från hushållet till byggnaden och sedan över till kommunal nivå i syfte att identifiera miljöeffekter globalt. Studien är tvärvetenskaplig i den bemärkelse att den innehåller användning av teori och metoder för analys och insamling av information som traditionellt används inom olika vetenskapliga discipliner. Ett systemtänkande tillämpas som bygger på idén om att utföra en analys av passivhusens energisystem som sträcker sig över flera systemnivåer. Den första systemnivån där studien startar är hushållsnivån, den innefattar hyresgästerna och de termiska laster som genereras från vardagliga hushållsaktiviteter. Från hushållsnivån går studien vidare upp till byggnadsnivån där egenskaperna hos själva byggnaderna samt värme och ventilationssystem analyseras inom ramen för inomhusklimat och energibalans i byggnaden. Slutligen nås den lokala nivån för att ta reda på vilka motiv som fanns för kommunen och bostadsbolaget att investera i passivhus från första början. Här ingår även en optimeringsstudie om effekterna av en omfattande ombyggnation till passivhus. De olika nivåerna i energisystemet som beskrevs här illustreras i figuren nedan.
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| 17. |
- Lagerlöf, Ingemar, et al.
(författare)
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No excess long-term mortality in stage I-IIA Hodgkin lymphoma patients treated with ABVD and limited field radiotherapy
- 2020
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Ingår i: British Journal of Haematology. - : John Wiley & Sons. - 0007-1048 .- 1365-2141. ; 188:5, s. 685-691
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Tidskriftsartikel (refereegranskat)abstract
- When treating limited stage classical Hodgkin lymphoma (cHL), balancing treatment efficacy and toxicity is important. Toxicities after extended-field radiotherapy are well documented. Investigators have aimed at reducing toxicity without compromising efficacy, mainly by using combined modality treatment (CMT), i.e. chemotherapy and limited-field radiotherapy. In some clinical trials, radiotherapy has been omitted. We evaluated 364 patients with stage I-IIA cHL treated between 1999 and 2005. Patients were treated with two or four cycles of doxorubicin, bleomycin, vinblastine and dacarbazine (ABVD) according to presence of risk factors, followed by 30 Gy limited-field (reduced compared to involved-field) radiotherapy. After a median follow-up of 16 years for survival, freedom from progression at five and ten years was 93% and overall survival at 5 and 10 years was 98% and 96%, respectively. Only two relapses, out of 27, occurred after more than 5 years. There was no excess mortality compared to the general population. Of the analysed subgroups, only patients with progression within five years showed significant excess mortality. The absence of excess mortality questions the concept of omitting radiotherapy after short-term chemotherapy, a strategy that has been associated with an elevated risk of relapse but not yet with a proven reduced long-term excess mortality.
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| 18. |
- Larsson, Magnus, et al.
(författare)
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Interaktivt lärande : om forskning och utveckling i samverkan
- 2010
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Ingår i: <em></em>Entreprenörskap i regioners tjänst. - Jönköping : Internationella handelshögskolan. Institutet för upplevelseindustrins utveckling. - 9789197919326 ; , s. 211-236
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Bokkapitel (övrigt vetenskapligt/konstnärligt)
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| 19. |
- Larsson, Magnus, et al.
(författare)
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Krattad manege vid Blå lagunen?
- 2012
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Ingår i: I de stora trendernas brus. - Visby : Gotland University Press. - 9789186343149 ; , s. 299-370
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Bokkapitel (refereegranskat)
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| 20. |
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| 21. |
- Lind, Sara Bergström, et al.
(författare)
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Detection of tyrosine phosphorylated proteins by combination of immunoaffinity enrichment, two-dimensional difference gel electrophoresis and fluorescent Western blotting
- 2010
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Ingår i: Biochemical and Biophysical Research Communications - BBRC. - : Elsevier BV. - 0006-291X .- 1090-2104. ; 401:4, s. 581-585
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Tidskriftsartikel (refereegranskat)abstract
- We describe fluorescence-based 2-D gel electrophoresis methods for visualization of low abundant cancer relevant tyrosine phosphorylated (pTyr) proteins The methods investigated were fluorescent Western blotting and two-dimensional difference gel electrophoresis (2-D DIGE) for detection of non-enriched and immunoaffinity enriched pTyr protein patterns The same anti-phosphotyrosine specific antibody 4G10 was used for both approaches The results from fluorescent Western blotting of total proteins and from enriched CyDye DIGE pre-labeled pTyr proteins showed similar down regulation of phosphorylation upon treating of cells from a cancer model system (K562 chronic myeloid leukemia cells) with imatinib This treatment introduced a known perturbation of phosphorylation that enabled testing of these new approaches to analyze variations in tyrosine phosphorylation levels Enrichment of pTyr proteins was found highly advantageous for the outcome Out of a simplified 2 D DIGE experiment of immunoaffinity enriched control and treated pTyr proteins differential analysis as well as protein identification by mass spectrometry (MS) was possible.
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| 22. |
- Lundgren, Magnus, et al.
(författare)
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Coxsackievirus B3 infection and PBDE exposure causes organ-specific effects on CYP-gene expression in the mouse
- 2007
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Ingår i: Toxicology. - : Elsevier BV. - 0300-483X .- 1879-3185. ; 242:1-3, s. 91-99
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Tidskriftsartikel (refereegranskat)abstract
- Common viral infections have been shown to change the tissue distribution of xenobiotics, including polybrominated diphenyl ethers (PBDEs). In previous studies, it has been shown that CYP2B gene expression is induced after PBDE exposure whereas coxsackievirus B3 (CBV3) infection suppresses the expression of CYP-gene expression in the liver. In the present study, CVB3 adapted to Balb/c mice was used to study the combined effects of infection and exposure to pure BDE-99 or the commercial mixture Bromkal on CYP1A1 and CYP2B expression in the lungs and pancreas on day 3 of the infection. The quantitative gene expression of virus, CYP1A1 and CYP2B was measured by real-time polymerase chain reaction (RT-PCR). PBDE exposure in the non-infected mice tended to increase CYP2B expression in the lungs but not in the pancreas. Infection in both non-exposed and PBDE-exposed mice increased CYP2B expression in the lungs but was non-detectable in the pancreas. In the non-infected mice PBDE exposure left the CYP1A1 expression unaltered in both the lungs and pancreas. Infection in both non-exposed and PBDE-exposed mice tended to decrease the gene expression of CYP1A1 in the lungs but to induce it in the pancreas. A correlation between the amount of virus and the gene expression of CYP2B was found in the lungs. However, no effects of PBDE on virus replication were observed in any organ. In conclusion, viral infection affects CYP-gene expression differently in the pancreas and lungs whereas PBDE-induced effects were not obvious. The organ-specific change in gene expression could explain a changed tissue distribution of xenobiotics during infection.
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| 23. |
- Lundgren, Magnus, et al.
(författare)
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Viral infection and PBDE exposure interact on CYP gene expression and enzyme activities in the mouse liver
- 2007
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Ingår i: Toxicology. - : Elsevier BV. - 0300-483X .- 1879-3185. ; 242:1-3, s. 100-108
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Tidskriftsartikel (refereegranskat)abstract
- In the present study coxsackievirus B3 (CVB3) adapted to Balb/c mice was used to examine whether infection affects xenobiotic-metabolising CYP1A1 and CYP2B gene expression (measured by RT-PCR) and the corresponding enzyme activities of ethoxyresorufin-O-deethylase (EROD) and pentoxyresorufin-O-depentylase (PROD), as observed on day 3 of infection. To study the simultaneous effects of xenobiotic exposure, mice were administered the polybrominated diphenyl ether (PBDE) compounds BDE-99 (single congener) and Bromkal 70-5 DE (commercial mixture). Serum thyroxine levels were also measured. High numbers of CVB3 were found in the livers of infected mice but no significant effects of PBDE on virus replication were observed. In infected mice gene expression and CYP activities were decreased in comparison with non-infected mice, especially for CYP2B. PBDE exposure in the non-infected mice was characterised by an increase in both CYP2B and PROD levels/activities, whereas CYP1A levels increased and EROD activity decreased. In general, PBDE exposure in the infected mice did not increase EROD and PROD activities to the same extent as in the non-infected exposed mice. Infected mice exposed to BDE-99 showed significantly higher CYP2B and PROD levels than both the infected non-exposed and Bromkal-exposed groups. T(4) levels were greatly decreased by infection and a tendency of reduced T(4) levels after PBDE exposure could be observed in non-infected mice. In conclusion, infection reduced the detoxifying capacity of the liver and the serum T(4) levels. PBDE exposure can modify these effects. Notably, in the infected mice differences between BDE-99 and Bromkal were observed on CYP2B gene expression and PROD activity.
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| 24. |
- Mandic, Aleksandra, et al.
(författare)
-
Cisplatin induces the proapoptotic conformation of Bak in a deltaMEKK1-dependent manner
- 2001
-
Ingår i: Molecular and Cellular Biology. - 0270-7306 .- 1098-5549. ; 21:11, s. 3684-3691
-
Tidskriftsartikel (refereegranskat)abstract
- In a panel of four human melanoma cell lines, equitoxic doses of cisplatin induced the proapoptotic conformation of the Bcl-2 family protein Bak prior to the execution phase of apoptosis. Because cisplatin-induced modulation of the related Bax protein was seen in only one cell line, a degree of specificity in the signal to Bak is indicated. Little is known about upstream regulation of Bak activity. In this study, we examined whether the apoptosis-specific pathway mediated by a kinase fragment of MEKK1 (DeltaMEKK1) is involved in the observed Bak modulation. We report that expression of a kinase-inactive fragment of MEKK1 (dominant negative MEKK [dnMEKK]) efficiently blocked cisplatin-induced modulation of Bak and cytochrome c release and consequently also reduced DEVDase activation and nuclear fragmentation. Accordingly, expression of a kinase-active MEKK1 fragment (dominant positive MEKK) was sufficient to induce modulation of Bak in three cell lines and to induce apoptosis in two of these. dnMEKK did not block cisplatin-induced c-Jun N-terminal kinase (JNK) activation, in agreement with a specifically proapoptotic role for the DeltaMEKK1 pathway. Finally, we show that reduction of Bak expression by antisense Bak reduced cisplatin-induced loss of mitochondrial integrity and caspase cleavage activity in breast cancer cell lines. In summary, we have identified Bak as a cisplatin-regulated component downstream in a proapoptotic, JNK-independent DeltaMEKK1 pathway.
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| 25. |
- Molin, Adam, et al.
(författare)
-
A Study on the Sensitivity Matrix in Power System State Estimation by Using Sparse Principal Component Analysis
- 2016
-
Ingår i: 2016 IEEE 55th Conference on Decision and Control, CDC 2016. - : Institute of Electrical and Electronics Engineers (IEEE). - 9781509018376 ; , s. 1529-1535
-
Konferensbidrag (refereegranskat)abstract
- This paper analyzes the joint impact of uncertainties in the input data on the power system state estimator. The approach is based on the sensitivity analysis of the estimated telemetry data with respect to the measurement data and the branch parameters with the main goal of locating relevant input components. In order to find relevant inputs, we analyze the normalized sensitivity matrix by sparse principal component analysis (PCA). The non-zero entries of the loading vectors related to the dominant principal components are considered to be the relevant inputs to the state estimator as they mainly contribute to the amplification of the estimated values. It turns out that PCA shows an elementary structure of the sensitivity matrix: All non-zero entries of a loading vector corresponding to a positive singular value belong either to the telemetry data or to the branch data. We show that this property is also valid for PCA with different sparsity-promoting constraints on the loading vector. The proposed analysis method is demonstrated by a numerical study.
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| 26. |
- Molin, Fredrik, 1977-, et al.
(författare)
-
Can the Human Resources Index (HRI) Be Used as a Process Feedback Measurement in a Structured Support Model for Systematic Work Environment Management?
- 2021
-
Ingår i: International Journal of Environmental Research and Public Health. - : MDPI. - 1661-7827 .- 1660-4601. ; 18:12
-
Tidskriftsartikel (refereegranskat)abstract
- The aim of the study was to estimate the level of the human resources index (HRI) measure among Swedish municipal employees, and to investigate the association between human resources index (HRI) and relational justice, short-term recovery, work environment-related production loss, and health-related production loss. A cross-sectional design was used with one sample of municipal employees (n = 6402). The results showed a positive association (r = 0.31) between human resources index (HRI) and relational justice; a positive (r = 0.27) association between HRI and short-term recovery; a negative association between HRI and work environment-related production loss (r = -0.37); and a negative association between HRI and health-related production loss (r = -0.23). The findings implicate that HRI captures important aspects of the work environment such as productivity, relational justice, and short-term recovery. The HRI measure is part of a support model used in workplaces to systematically address work environment-related issues. Monitoring changes in the HRI measure, it is possible to determine whether the measures taken effect production loss, perceived leadership, and short-term recovery in a work group. The support model using HRI may thus be used to complement traditional work environment surveys conducted in Swedish organizations as obliged by legal provisions.
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| 27. |
- Molin, Fredrik, 1977-, et al.
(författare)
-
First-Line Managers' Experiences of Working with a Structured Support Model for Systematic Work Environment Management
- 2020
-
Ingår i: International Journal of Environmental Research and Public Health. - : MDPI. - 1661-7827 .- 1660-4601. ; 17:11
-
Tidskriftsartikel (refereegranskat)abstract
- Background: This paper describes the experiences of first-line managers when working with a structured support model for systematic work environment management in their work groups. First-line managers play a key part in influencing the work environment. Methods: In this study, a sample of managers implementing a structured support model, the Stamina model, in Swedish municipalities were interviewed. A total of 31 (n = 31) interviews were conducted at two time points during a one-year period. The collected data were analysed using a qualitative thematic approach. Results: The results showed that managers experienced discomfort when giving the responsibility of working with work environmental issues to employees. However, managers also experienced and were impressed by how well it worked in allowing employees to take on work environmental issues. Managers found that they balanced between being quiescent and, at the same time, actively monitoring progress in the work groups. Conclusions: The results from this study implicate that managers need to be sensitive to the needs and capacity of their work groups. The oracle in Delphi stated know yourself. We conclude: Know your group!
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| 28. |
- Molin, Fredrik, 1977-, et al.
(författare)
-
Implementing New Working Practices Through a Structured Support Model for Systematic Work Environment Management : A Qualitative Study From Swedish Municipalities
- 2021
-
Ingår i: Journal of Occupational and Environmental Medicine. - : Lippincott Williams & Wilkins. - 1076-2752 .- 1536-5948. ; 63:5, s. E259-E266
-
Tidskriftsartikel (refereegranskat)abstract
- Objective: To describe factors that facilitate and hinder implementation of a structured support model focusing on psychosocial work environment in Swedish municipalities.Methods: This qualitative study was conducted in six Swedish municipalities. In total, 63 semi-structured interviews and focus groups were conducted with key stakeholders in the municipalities during a 2-year period. The consolidated framework for implementation research (CFIR) was used for data analysis.Results: Findings suggest that the support model needs to have an easy enough structure to understand and use, and it must be immediately applicable for work groups and employees. An important factor for facilitating implementation was the support from first-line managers and upper management.Conclusions: Upper management need to be committed to the model and communicate clearly to lower levels of the implementing organization on the purpose and objectives of the model.
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| 29. |
- Molin, Hanna, et al.
(författare)
-
Automated data transfer for digital twin applications : Two case studies
- 2024
-
Ingår i: Water environment research. - : John Wiley and Sons Inc. - 1061-4303 .- 1554-7531. ; 96:7
-
Tidskriftsartikel (refereegranskat)abstract
- Digital twins have been gaining an immense interest in various fields over the last decade. Bringing conventional process simulation models into (near) real time are thought to provide valuable insights for operators, decision makers, and stakeholders in many industries. The objective of this paper is to describe two methods for implementing digital twins at water resource recovery facilities and highlight and discuss their differences and preferable use situations, with focus on the automated data transfer from the real process. Case 1 uses a tailor-made infrastructure for automated data transfer between the facility and the digital twin. Case 2 uses edge computing for rapid automated data transfer. The data transfer lag from process to digital twin is low compared to the simulation frequency in both systems. The presented digital twin objectives can be achieved using either of the presented methods. The method of Case 1 is better suited for automatic recalibration of model parameters, although workarounds exist for the method in Case 2. The method of Case 2 is well suited for objectives such as soft sensors due to its integration with the SCADA system and low latency. The objective of the digital twin, and the required latency of the system, should guide the choice of method. Practitioner Points: Various methods can be used for automated data transfer between the physical system and a digital twin. Delays in the data transfer differ depending on implementation method. The digital twin objective determines the required simulation frequency. Implementation method should be chosen based on the required simulation frequency.
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| 30. |
- Molin, Hanna, et al.
(författare)
-
Automated data transfer for digital twin applications : Two case studies
- 2024
-
Ingår i: Water environment research. - : John Wiley & Sons. - 1061-4303 .- 1554-7531. ; 96:7
-
Tidskriftsartikel (refereegranskat)abstract
- Digital twins have been gaining an immense interest in various fields over the last decade. Bringing conventional process simulation models into (near) real time are thought to provide valuable insights for operators, decision makers, and stakeholders in many industries. The objective of this paper is to describe two methods for implementing digital twins at water resource recovery facilities and highlight and discuss their differences and preferable use situations, with focus on the automated data transfer from the real process. Case 1 uses a tailor-made infrastructure for automated data transfer between the facility and the digital twin. Case 2 uses edge computing for rapid automated data transfer. The data transfer lag from process to digital twin is low compared to the simulation frequency in both systems. The presented digital twin objectives can be achieved using either of the presented methods. The method of Case 1 is better suited for automatic recalibration of model parameters, although workarounds exist for the method in Case 2. The method of Case 2 is well suited for objectives such as soft sensors due to its integration with the SCADA system and low latency. The objective of the digital twin, and the required latency of the system, should guide the choice of method.Practitioner Points Various methods can be used for automated data transfer between the physical system and a digital twin. Delays in the data transfer differ depending on implementation method. The digital twin objective determines the required simulation frequency. Implementation method should be chosen based on the required simulation frequency. This paper showcases two case studies for automated data transfer in digital twins. One uses a tailor-made infrastructure for data transfer between the facility and digital twin, the other uses edge computing. The digital twin objective should determine the required simulation frequency and, thus, sets boundaries on suitable implementation methods. image
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| 31. |
- Molin, Hanna, et al.
(författare)
-
VA-kluster Mälardalen 2019-2021 - Slutrapport
- 2022
-
Rapport (populärvet., debatt m.m.)abstract
- VA-kluster Mälardalen – ett högt värderat forsknings- och utbildningsklusterVA-kluster Mälardalen är ett viktigt och mycket uppskattat nätverk. Det vittnar våra medlemmar om. Som en del i arbetet med slutrapporteringen ställdes två frågor till alla medlemmar: ”Varför är ni medlemmar i VA-kluster Mälardalen?” och ”Vad betyder VA-kluster Mälardalen för er?”. Svaren visar att det våra medlemmar uppskattar mest är det nätverk som klustret utgör och den forskning vi bedriver tillsammans. Nätverket är kärnan i klustret, och tack vare det kan vi genomföra högklassig forskning med såväl akademisk höjd som praktiskt tillämpbara resultat. Den naturliga kontakten mellan de tretton VA-organisationerna och de sju forskande parterna gynnar VA-forskningen i Sverige och driver teknik- och kunskapsutvecklingen framåt.Klustret ger VA-organisationerna en möjlighet att delta i projekt som genererar direkt nytta i verksamheten och som kan svara på frågor kopplat till de utmaningar de står inför – både på kort och lång sikt. De forskande parterna uppskattar att kunna arbeta med forskning som kommer till användning ”på riktigt”. Klustret ger också möjligheter för alla medlemmar att delta och driva projekt som de saknar kompetens eller ekonomiska förut-sättningar att driva ensamma. Den samlade projektvolymen inom VA-kluster Mälardalen har totalt uppgått till drygt 142 MSEK under programperioden 2019–2021. Det motsvarar en uppväxling av SVU- och SIVL-medlen med 17 gånger. Den faktiska projektfinansieringen speglar en del av nyttan med VA-kluster Mälardalen, men för att få en komplett bild bör det även beaktas att det totalt har genomförts närmare 100 forsknings- och utvecklingsprojekt under program-perioden, och att 17 av våra 20 medlemmar har varit direkt involverade i ett eller flera av dessa projekt. Många av projekten har dessutom genomförts i samarbete med andra VA-organisationer utanför klustret.Resultaten från denna uppsjö av forsknings-projekt kan knappast sammanfattas på ett rättvist sätt i en slutrapport, än mindre i en sammanfattning som denna. Ett urval av de forskningsprojekt som helt eller delvis finansieras av SVU-medel genom VA-kluster Mälardalen redovisas i slutrapporten, men i övrigt hänvisar vi till vetenskapliga artiklar och andra rapporter enligt de referenslistor som finns både i slutrapporten och de verksamhetsberättelser som har publicerats årligen under programperioden (tillgängliga via klustrets hemsida www.va-malardalen.se).
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| 32. |
- Molin, Johanna, et al.
(författare)
-
Neonatal outcome following metformin-treated gestational diabetes mellitus : a population-based cohort study
- 2024
-
Ingår i: Acta Obstetricia et Gynecologica Scandinavica. - : John Wiley & Sons. - 0001-6349 .- 1600-0412. ; 103:5, s. 992-1007
-
Tidskriftsartikel (refereegranskat)abstract
- Introduction: Neonatal hypoglycemia is a common complication associated with gestational diabetes and therefore relevant to consider in evaluations of maternal treatment. We aimed to investigate the risk of neonatal hypoglycemia in offspring exposed to metformin treatment alone (MT) or combined with insulin (MIT) in comparison with nutrition therapy alone (NT), and insulin treatment alone (IT). In addition, we investigated MT in comparison with MIT. Secondary outcomes included neonatal anthropometrics, respiratory morbidity, hyperbilirubinemia, 5-min Apgar score, and preterm birth.Material and methods: This Swedish population-based cohort included 16 181 women diagnosed with gestational diabetes, and their singleton offspring born in 2019–2021. We estimated risk as adjusted odds ratio (aOR) with 95% confidence interval (CI), using individual-level, linkage register-data in multivariable logistic regression models.Results: In the main analysis, MT was associated with a lower risk of neonatal hypoglycemia versus NT (aOR 0.85, 95% CI: 0.74–0.96), versus MIT (0.74 [0.64–0.87]), and versus IT (0.47 [0.40–0.55]), whereas MIT was associated with a similar risk of neonatal hypoglycemia versus NT (1.14 [0.99–1.30]) and with lower risk versus IT (0.63 [0.53–0.75]). However, supplemental feeding rates were lower for NT versus pharmacological treatments (p < 0.001). In post hoc subgroup analyses including only exclusively breastfed offspring, the risk of neonatal hypoglycemia was modified and similar among MT and NT, and higher in MIT versus NT. Insulin exposure, alone or combined with metformin, was associated with increased risk of being large for gestational age. Compared with NT, exposure to any pharmacological treatment was associated with significantly lower risk of 5-min Apgar score < 4. All other secondary outcomes were comparable among the treatment categories.Conclusions: The risk of neonatal hypoglycemia appears to be comparable among offspring exposed to single metformin treatment and nutrition therapy alone, and the lower risk that we observed in favor of metformin is probably explained by a difference in supplemental feeding practices rather than metformin per se. By contrast, the lower risk favoring metformin exposure over insulin exposure was not explained by supplemental feeding. However, further investigations are required to determine whether the difference is an effect of metformin per se or mediated by other external factors.
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| 33. |
- Molin, Magnus
(författare)
-
Adenovirus vector systems permitting regulated protein expression and their use for in vivo splicing studies
- 2001
-
Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- We have constructed two adenovirus-based gene expression vector systems permitting regulated protein expression. They are based on the tetracycline-regulated Tet-ON- and the progesterone antagonist RU 486-regulated gene expression systems, which were rescued into E1-deficient adenovirus vectors. The vectors function in a number of cell types representing a broad species-variety and the regulation of protein expression was shown to be tightly controlled in cells not permissive for virus replication. Furthermore, the adenovirus-Tet-ON system was shown to perform in mice after intramuscular administration.The novel adenovirus-vector systems were then used to study the effects of overexpression of selected proteins on adenovirus replication during a lytic infection, with focus on regulation of adenovirus alternative splicing. Expression of adenovirus transcription units is to a large extent temporally regulated at the level of alternative pre-mRNA splicing, where viral splice site usage shifts from proximal to distal splice site selection as infection proceeds. This makes adenovirus an appropriate model for mechanistic studies of regulated splicing. We show that overexpression of the essential host cell splicing factor ASF/SF2 inhibits this shift by promoting usage of proximal splice sites. As a consequence, the virus displayed a markedly inhibited growth. Interestingly, mRNA expression from the adenovirus major late promoter was almost completely lost as a consequence of ASF/SF2 overexpression. Collectively, the cellular splicing factor ASF/SF2 prevents adenovirus from entering the late phase of infection. This strongly argues for a need for the virus to block the splicing enhancer activity of ASF/SF2 for establishment of a lytic infection. Further, from analysis of the strict inhibition of late region 1 late pre-mRNA splicing we propose that the temporal regulation of alternative splicing is merely a consequence of fitness rather than profoundly deleterious effects of an unregulated expression. During our studies we noted that in 293 cells, which are used for growth of E1-deficient Ad vectors, an unwanted background reporter gene expression was evident in our vector systems. We therefore introduced an additional regulatory element, functioning as a transcriptional road-block, and showed that this methodological innovation represents a way to overcome the potentially deleterious effects of background reporter gene expression. This modified viral vector system should make it possible to reconstruct recombinant viruses expressing highly toxic proteins.In conclusion, this work presents a new in vivo model system to study proteins involved in RNA splicing and other gene regulatory mechanisms.
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| 34. |
- Molin, Magnus, et al.
(författare)
-
Biomarkers of chronic airflow limitation and COPD identified by mass spectrometry
- 2024
-
Ingår i: ERJ Open Research. - : European Respiratory Society. - 2312-0541. ; 10
-
Tidskriftsartikel (refereegranskat)abstract
- RATIONALE: COPD affects 300 million people worldwide and is the third leading cause of death according to World Health Organization global health estimates. Early symptoms are subtle, and so COPD is often diagnosed at an advanced stage. Thus, there is an unmet need for biomarkers that can identify individuals at early stages of the disease before clinical symptoms have manifested. To date, few biomarkers are available for clinical diagnostic use in COPD.METHODS: We evaluated a panel of serum biomarkers related to inflammation and infection for their ability to discriminate between 77 subjects with chronic airflow limitation (CAL) and 142 subjects with COPD, versus 150 healthy subjects (divided into two control groups that were matched with regards to age, gender and smoking to CAL and COPD). Healthy subjects and CAL were from Burden of Obstructive Lung Disease (BOLD), a population-based study. CAL was defined by post-bronchodilatory forced expiratory volume in 1 s/forced vital capacity ratio <0.7 in the BOLD population. COPD subjects were from Tools for Identifying Exacerbations (TIE), a COPD patient cohort. Quantification of 100 biomarker candidates was done by liquid chromatography-tandem mass spectrometry.RESULTS: Several protein-derived peptides were upregulated in CAL, compared to controls; most notably peptides representing histidine-rich glycoprotein (HRG), α1-acid glycoprotein (AGP1), α1-antitrypsin (α1AT) and fibronectin. Out of these, HRG-, AGP1- and α1AT-specific peptides were also elevated in the COPD cohort.CONCLUSION: HRG, AGP1 and α1AT biomarkers distinguish subjects with CAL and COPD from healthy controls. HRG and AGP1 represent novel findings.
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| 35. |
- Molin, Magnus, et al.
(författare)
-
Overexpression of essential splicing factor ASF/SF2 blocks the temporalshift in adenovirus pre-mRNA splicing and reduces virus progeny formation
- 2000
-
Ingår i: Journal of Virology. - 0022-538X .- 1098-5514. ; 74:19, s. 9002-9009
-
Tidskriftsartikel (refereegranskat)abstract
- Expression of cytoplasmic mRNA from most adenovirus transcription units is subjected to a temporal regulation at the level of alternative pre-mRNA splicing. The general tendency is that splice site selection changes from proximal to distal late after infection. Interestingly, ASF/SF2, which is a prototypical member of the SR family of splicing factors, has the opposite effect on splice site selection, inducing an increase in proximal splice site usage. We have previously shown that SR proteins late during an adenovirus infection become partially inactivated as splicing regulatory proteins. A prediction from these results is that overexpression of an SR protein, such as ASF/SF2, during virus growth will interfere with virus replication by disturbing the balance of functional and nonfunctional ASF/SF2 in the infected cell. To test this hypothesis, we reconstructed a recombinant adenovirus expressing ASF/SF2 under the transcriptional control of a regulated promoter. The results show that, as predicted, induction of ASF/SF2 during lytic virus growth prevents the early to late shift in mRNA expression from both early (E1A and E1B) and late (L1) transcription units. Furthermore, ASF/SF2 overexpression blocks viral DNA replication and reduces selectively cytoplasmic accumulation of major late mRNA, resulting in a lower virus yield. Collectively, our results provide additional support for the hypothesis that viral control of SR protein function is important for the proper expression of viral proteins during lytic virus growth.
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| 36. |
- Molin, Magnus, et al.
(författare)
-
Two Novel Adenovirus Vector Systems Permitting Regulated Protein Expression in Gene Transfer Experiments
- 1998
-
Ingår i: Journal of Virology. - 0022-538X .- 1098-5514. ; 72:10, s. 8358-8861
-
Tidskriftsartikel (refereegranskat)abstract
- Two new adenovirus vector systems based on the tetracycline-regulated Tet-ON- (Gossen, M., et al., Science 268:1766-1769, 1995) and the RU 486-regulated progesterone antagonist (Wang, Y., et al., Proc. Natl. Acad. Sci. USA 91:8180-8184, 1994)-induced gene expression systems are described. We show that both systems permit a tight control of chloramphenicol acetyltransferase reporter gene expression in a variety of cell types, with induction levels of approximately 1,800-fold (Tet-ON system) and 600-fold (RU 486-regulated system), respectively. A significant advantage of our vector systems is that reporter protein expression can be adjusted over a wide range by varying the amount of inducer. The Tet-ON system is also shown to permit an efficient control of reporter gene expression in mice.
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| 37. |
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| 38. |
- Molin, Magnus, et al.
(författare)
-
Unscheduled expression of capsid protein IIIa results in defects in adenovirus major late mRNA and protein expression
- 2002
-
Ingår i: Virus Research. - 0168-1702 .- 1872-7492. ; 83:1-2, s. 197-206
-
Tidskriftsartikel (refereegranskat)abstract
- Adenovirus gene expression is to a large extent regulated at the level of alternative RNA splicing. For example, in the major late region 1 (L1) unit, a common 5' splice site can be joined to two alternative 3' splice sites, resulting in the formation of the so-called 52,55K (proximal 3' splice site) or the IIIa (distal 3' splice site) mRNAs. Whereas, the 52,55K mRNA is expressed both early and late during infection, the IIIa mRNA is strictly confined to the late phase of the infectious cycle. We have previously shown that IIIa mRNA splicing is subjected to a tight viral control of IIIa 3 splice site usage. In an attempt to determine why adenovirus uses elaborate mechanisms to confine IIIa mRNA production to the late phase of infection, we characterized the phenotype of a recombinant adenovirus expressing the IIIa protein from an inducible tetracycline regulated gene cassette. The results show that expression of the IIIa protein during the early phase of infection results in a significant reduction in late viral protein synthesis and a moderate block to viral DNA replication. Interestingly, unscheduled IIIa protein expression resulted in a perturbation of the accumulation of alternatively spliced L1 mRNAs. Thus, 52,55K mRNA accumulation was inhibited while no effects on endogenous IIIa mRNA expression was detected.
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| 39. |
- Notini Burch, Cecilia, 1981-
(författare)
-
A Cold War Pursuit : Soviet refugees in Sweden, 1945-54
- 2014
-
Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- What determines refugee policies in liberal democracies? Humanitarianism? International relations? Economics? Identity issues? International law? Concerns for national security? This book explores these factors through a case study of non-aligned Sweden’s management of Soviet refugees during the first decade of the Cold War. The policy of admission and political asylum; the government’s handling of direct Soviet demands regarding refugees; the Swedish authorities’ surveillance strategies and the continuing living conditions of individuals who were permitted to stay, are all studied in depth. The results demonstrate that refugees’ right to protection was successively strengthened during the period, as asylum policies were reformulated as a matter more of (international) law than (national) politics. That said, however, some refugees – Russian speakers in particular – were generally regarded as more untrustworthy than others and were subjected to severe control measures, such as, for example, rigorous restrictions placed on their movement. The treatment they received fluctuated significantly in tandem with the bipolar tensions of the Cold War. Here, ethnic prejudice influenced perceptions of threat.This book thus contributes to our understanding of the Cold War and the considerable impact it had on widespread aspects of society. However, it also facilitates a more thorough comprehension of the fundamental prerequisites of refugee policies in general. In particular, it pertains to the paramount question: what are the conditions for humanitarian policies in times of international tension? This is a highly relevant issue in the post 9/11 world in which security concerns and migration policies are firmly entangled, and where counter-terrorism measures have increased the difficulties faced by all refugees who strive to reach the West.
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| 40. |
- Pera, Susanna, et al.
(författare)
-
Development Work in Healthcare : What Supportive and Deterrent Factors Do Employees Working in a Hospital Department Experience in an Improved Work Environment?
- 2021
-
Ingår i: International Journal of Environmental Research and Public Health. - : MDPI. - 1661-7827 .- 1660-4601. ; 18:16
-
Tidskriftsartikel (refereegranskat)abstract
- Work-related mental health issues, accounting for high worker absenteeism in the world's developed economies, are increasing, with the main cause being workplace conditions. The health services sector is especially experiencing great problems with this, because of challenging psychosocial working conditions. The aim of this study was to explore employees' experiences of development work with a focus on the work environment within a hospital department with an outspoken special development assignment. The special assignment was decided by the highest management at the hospital and concerned work environment, caring processes, and ways of organizing the work. Eleven employees completed two individually semi-structured interviews, approximately 7 and 13 months after the start of the special assignment at the department. Interviews were analyzed using thematic analysis. The results reveal that both internal and external aspects influence the development work and highlight the importance of viewing the local development work in relation to how the rest of the organization functions. Important factors and conditions for a supportive and change-friendly work culture are discussed, as well as the need to plan for integration and change to create conditions for successful implementation of the results from organizational development and change initiatives.
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| 41. |
- Persson, Sofia, et al.
(författare)
-
Validation of an optimised method for quantitative detection of hepatitis E virus in pork sausage
-
Annan publikation (övrigt vetenskapligt/konstnärligt)abstract
- Hepatitis E virus (HEV) is an emerging zoonosis that can be transmitted to humans through the consumption of raw or undercooked pork meat products. Several methods for detecting the virus in food have been described, but there are still few robust data on qualitative and quantitative performance characteristics. In this study, we developed an optimised workflow for quantitative detection of HEV in pork sausage based on a combination of previously existing protocols. The protocol uses sample disruption and phase separation with tri-reagent and 1-bromo-3-chloropropane, followed by RNA concentration with isopropanol precipitation. We validated the protocol for use on reverse transcription quantitative real-time PCR (RT-qPCR) and reverse transcription droplet digital (RT-ddPCR). The 95% limit of detection and limit of quantification was 200 copies/g for both RT-qPCR and RT-ddPCR. The RT-ddPCR technology has previously shown promise as a more precise alternative to RT-qPCR. However, we found no evidence for improved performance using RT-ddPCR instead of RT-qPCR in this method. Furthermore, we also evaluated different combinations of RNA concentration methods and PCR detection strategies. This showed that isopropanol precipitation of viral RNA was more than twice as efficient as magnetic silica bead-based extraction when an inhibitor tolerant RT-PCR detection strategy was used. In conclusion, we present an efficient and well-characterised method for quantitative detection of HEV in pork sausage. Such methods are valuable to provide high quality data for risk assessments and food monitoring.
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| 42. |
- Tideman, Magnus, 1959, et al.
(författare)
-
Detta är ingen skola för alla
- 2007
-
Ingår i: Göteborgs-Posten, 2007-06-10.
-
Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)
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| 43. |
- Viktorsson, K, et al.
(författare)
-
Increased apoptosis and increased clonogenic survival of 12V-H-ras transformed rat fibroblasts in response to cisplatin
- 2000
-
Ingår i: Apoptosis (London). - 1360-8185 .- 1573-675X. ; 5:4, s. 355-367
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Tidskriftsartikel (refereegranskat)abstract
- Mutationally activated Ras is involved in tumor progression and likely also in drug resistance. Using survival, viability and apoptosis assays, we have here compared the cisplatin sensitivities of FR3T3 rat fibroblasts and a 12V-H-ras transformed subline (Ras2:3). Around 24 h after cisplatin treatment Ras2:3 cells showed higher apoptosis levels and lower viability than FR3T3. This increased sensitivity correlated with weaker cisplatin-induced activation of Jun N-terminal kinase (JNK). In contrast to apoptosis assays, colony formation assays showed that Ras2:3 were more resistant to cisplatin than were FR3T3. This was partly due to the increased cisplatin sensitivity of FR3T3 seeded at low densities, as required in colony formation assays. In addition, Ras2:3 cisplatin survivors had a higher relative proliferative capacity. Cell cycle analyses showed that FR3T3 cells initially responded with a dose-dependent G2 arrest, while Ras2:3 accumulated in S-phase. Experiments with an anti-apoptotic mutant of MEKK1 suggested that the apoptotic response of Ras2:3 cells is not specific to the S-phase fraction. In summary, the cisplatin response of ras-transformed fibroblasts is distinct from that of parental cells, in that they show increased apoptosis, a different cell cycle response and increased post-treatment proliferative capacity. The results illustrate the need to carefully consider methods and protocols for in vitro studies on chemotherapy sensitivity.
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| 44. |
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| 45. |
- Xhagjika, Vamis, et al.
(författare)
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Structured Cloud federation for Carrier and ISP infrastructure
- 2014
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Ingår i: 2014 IEEE 3rd International Conference on Cloud Networking, CloudNet 2014. - 9781479927302 ; , s. 20-26
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Konferensbidrag (refereegranskat)abstract
- Cloud Computing in recent years has seen enhanced growth and extensive support by the research community and industry. The advent of cloud computing realized the concept of commodity computing, in which infrastructure (resources) can be allocated on demand giving the illusion of infinite resource availability. The state-of-art Carrier and ISP infrastructure technology is composed of tightly coupled software services with the underlying customized hardware architecture. The fast growth of cloud computing as a vastly consolidated and stabilized technology is appealing to Carrier Providers in order to reduce Carrier deployment costs and enable a future of Carrier Clouds with easily accessible virtual carriers. For such migration to happen software services need to be generalized, to decouple hardware and software, and prepared to move into the Cloud. The network backbone is centrally managed and only provides network connectivity. We believe this presents an opportunity. The edges of such networks and the core are interconnected with high performance links. If services could be deployed in these edges they would benefit from enhanced locality to the user. In this position paper we propose a distributed cloud architecture (precisely a structured multi-cloud federated infrastructure), with minimal impact on existing infrastructure, as a first step to incorporate the Cloud into the network infrastructure of such providers, enabling and enhancing novel and existing applications.
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| 46. |
- Zaigham, Suneela, et al.
(författare)
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Importance of type and degree of IgE sensitisation for defining fractional exhaled nitric oxide reference values
- 2021
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Ingår i: Respiratory Medicine. - : Elsevier. - 0954-6111 .- 1532-3064. ; 188
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Tidskriftsartikel (refereegranskat)abstract
- BackgroundFractional exhaled nitric oxide (FENO) is a marker of type 2 airway inflammation used in clinical practice in asthma. However, reference values are needed to broaden the clinical use of FENO and this is within the scope of a newly started Global Lung Function Initiative task force. We aim to study FENO levels with special emphasis on the upper limit of normal (ULN) in relation to the type and degree of IgE sensitisation.MethodsFENO was measured in 1855 non-smoking, respiratory healthy subjects from the Swedish CArdioPulmonary bioImage Study (SCAPIS). Atopic subjects (n = 424), defined as being IgE-sensitised to aeroallergens (ImmunoCAP Phadiatop™, ≥0.35 PAU/l) were compared to non-atopic subjects (<0.35 PAU/l, n = 1431). Atopic subjects were further characterised according to their grade of IgE sensitisation (IgE antibody tertiles: (T1<1.16, T2 1.16–3.72 and T3 >3.72 PAU/l) and sensitisation to perennial (cat or mite) or seasonal (birch) allergens.ResultsSubjects IgE-sensitised to cat or mite had higher FENO compared to non-atopic subjects (FENO (ppb): median 20.0 vs. 15.0, and ULN 50.4 vs. 33.0, p < 0.001). This was seen to a lesser extent for subjects IgE-sensitised to birch only (median 18.0 vs. 15.0, and ULN 38.0 vs. 33.0, p = 0.048). Atopic subjects with a high degree of IgE sensitisation (Phadiatop: >3.72 PAU/l) had the highest FENO compared to non-atopic subjects (median 20.0 vs. 15.0, and ULN 56.0 vs. 33.0, p < 0.001).ConclusionsThe type and degree of IgE sensitisation should be considered in generating FENO reference values.
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| 47. |
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| 48. |
- Åberg, Magnus, 1976-, et al.
(författare)
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Greenhouse Gas Emission from General District Heat use in Sweden: An Approach for Justified Comparisons in Residential Energy Use
- 2009
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Ingår i: Scientific Conference on Energy Saving and Green Energy, Älvsjömässan, Stockholm.
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Konferensbidrag (refereegranskat)abstract
- Significant uncertainties regarding the evaluation of environmental impact from the use ofenergy motivates the need for a unified evaluation approach. In this paper a marginalapproach for general evaluation of CO2 equivalent emissions from the use of district heat inSweden is proposed. A predefined national marginal heat production mix is used to define amarginal heat evaluation approach to calculate the greenhouse gas emissions from the use ofdistrict heating. The novel approach offers a method for justified comparisons of differenttypes of energy use. By using the marginal heat evaluation approach it was shown that theevaluation of the electricity used in heat production for district heating is significant for theCO2 equivalent emissions. A survey of general district heating CO2 emission evaluationexamples is also included in the paper. The marginal heat use approach generates high CO2equivalent emission values in comparison with earlier studies. The overall conclusion of thepaper is that there is a need for further research and discussion regarding development ofmethods for energy use evaluation. The general marginal heat use approach is part of thesolution to this problem.
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| 49. |
- Åström-Paulsson, Sofia, et al.
(författare)
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A Quantitative Study on Employees' Experiences of a Support Model for Systematic Work Environment Management in Swedish Municipalities
- 2023
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Ingår i: International Journal of Environmental Research and Public Health. - : MDPI. - 1661-7827 .- 1660-4601. ; 20:5
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Tidskriftsartikel (refereegranskat)abstract
- Today's working life is constantly changing, and work environmental risk factors can alter swiftly. Besides the traditional physical work environment risk factors, somewhat more abstract organizational and social work environment factors also play an ever-increasing role, both in preventing and causing work-related illness. This requires a preventive work environment management that can respond to rapid changes, and where the assessment and remedies rely more on employee participation than on predetermined threshold limits. This study aimed to investigate if the use of a support model (the Stamina model) for workplace improvements could render the same positive effects in quantitative measures that have previously been shown in qualitative studies. Employees from six municipalities used the model for 12 months. They answered a questionnaire at baseline and after six and 12 months, to detect any changes in how they characterized their current work situation and perceived their influence, productivity, short-term recovery, and organizational justice. The results showed that employees felt more influential in work situations related to communication/collaboration and roles/tasks at the follow-up compared to the baseline. These results are consistent with previous qualitative studies. We found no significant changes in the other endpoints. The results strengthen previous conclusions, namely that the Stamina model can be used as part of inclusive, modern, and systematic work environment management.
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