| 1. |
- Klionsky, Daniel J., et al.
(författare)
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Guidelines for the use and interpretation of assays for monitoring autophagy
- 2012
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Ingår i: Autophagy. - : Informa UK Limited. - 1554-8635 .- 1554-8627. ; 8:4, s. 445-544
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Forskningsöversikt (refereegranskat)abstract
- In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process vs. those that measure flux through the autophagy pathway (i.e., the complete process); thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from stimuli that result in increased autophagic activity, defined as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (in most higher eukaryotes and some protists such as Dictyostelium) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the field understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field.
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| 2. |
- Akperov, Mirseid, et al.
(författare)
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Cyclone Activity in the Arctic From an Ensemble of Regional Climate Models (Arctic CORDEX)
- 2018
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Ingår i: Journal of Geophysical Research: Atmospheres. - 2169-8996 .- 2169-897X. ; 123:5, s. 2537-2554
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Tidskriftsartikel (refereegranskat)abstract
- The ability of state-of-the-art regional climate models to simulate cyclone activity in the Arctic is assessed based on an ensemble of 13 simulations from 11 models from the Arctic-CORDEX initiative. Some models employ large-scale spectral nudging techniques. Cyclone characteristics simulated by the ensemble are compared with the results forced by four reanalyses (ERA-Interim, National Centers for Environmental Prediction-Climate Forecast System Reanalysis, National Aeronautics and Space Administration-Modern-Era Retrospective analysis for Research and Applications Version 2, and Japan Meteorological Agency-Japanese 55-year reanalysis) in winter and summer for 1981-2010 period. In addition, we compare cyclone statistics between ERA-Interim and the Arctic System Reanalysis reanalyses for 2000-2010. Biases in cyclone frequency, intensity, and size over the Arctic are also quantified. Variations in cyclone frequency across the models are partly attributed to the differences in cyclone frequency over land. The variations across the models are largest for small and shallow cyclones for both seasons. A connection between biases in the zonal wind at 200 hPa and cyclone characteristics is found for both seasons. Most models underestimate zonal wind speed in both seasons, which likely leads to underestimation of cyclone mean depth and deep cyclone frequency in the Arctic. In general, the regional climate models are able to represent the spatial distribution of cyclone characteristics in the Arctic but models that employ large-scale spectral nudging show a better agreement with ERA-Interim reanalysis than the rest of the models. Trends also exhibit the benefits of nudging. Models with spectral nudging are able to reproduce the cyclone trends, whereas most of the nonnudged models fail to do so. However, the cyclone characteristics and trends are sensitive to the choice of nudged variables.
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| 3. |
- Akperov, Mirseid, et al.
(författare)
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Future projections of cyclone activity in the Arctic for the 21st century from regional climate models (Arctic-CORDEX)
- 2019
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Ingår i: Global and Planetary Change. - : Elsevier BV. - 0921-8181 .- 1872-6364. ; 182
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Tidskriftsartikel (refereegranskat)abstract
- Changes in the characteristics of cyclone activity (frequency, depth and size) in the Arctic are analyzed based on simulations with state-of-the-art regional climate models (RCMs) from the Arctic-CORDEX initiative and global climate models (GCMs) from CMIP5 under the Representative Concentration Pathway (RCP) 8.5 scenario. Most of RCMs show an increase of cyclone frequency in winter (DJF) and a decrease in summer (JJA) to the end of the 21st century. However, in one half of the RCMs, cyclones become weaker and substantially smaller in winter and deeper and larger in summer. RCMs as well as GCMs show an increase of cyclone frequency over the Baffin Bay, Barents Sea, north of Greenland, Canadian Archipelago, and a decrease over the Nordic Seas, Kara and Beaufort Seas and over the sub-arctic continental regions in winter. In summer, the models simulate an increase of cyclone frequency over the Central Arctic and Greenland Sea and a decrease over the Norwegian and Kara Seas by the end of the 21st century. The decrease is also found over the high-latitude continental areas, in particular, over east Siberia and Alaska. The sensitivity of the RCMs' projections to the boundary conditions and model physics is estimated. In general, different lateral boundary conditions from the GCMs have larger effects on the simulated RCM projections than the differences in RCMs' setup and/or physics.
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| 4. |
- Akperov, Mirseid, et al.
(författare)
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Future projections of wind energy potentials in the arctic for the 21st century under the RCP8.5 scenario from regional climate models (Arctic-CORDEX)
- 2023
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Ingår i: Anthropocene. - 2213-3054. ; 44
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Tidskriftsartikel (refereegranskat)abstract
- The Arctic has warmed more than twice the rate of the entire globe. To quantify possible climate change effects, we calculate wind energy potentials from a multi-model ensemble of Arctic-CORDEX. For this, we analyze future changes of wind power density (WPD) using an eleven-member multi-model ensemble. Impacts are estimated for two periods (2020-2049 and 2070-2099) of the 21st century under a high emission scenario (RCP8.5). The multi-model mean reveals an increase of seasonal WPD over the Arctic in the future decades. WPD variability across a range of temporal scales is projected to increase over the Arctic. The signal amplifies by the end of 21st century. Future changes in the frequency of wind speeds at 100 m not useable for wind energy production (wind speeds below 4 m/s or above 25 m/s) has been analyzed. The RCM ensemble simulates a more frequent occurrence of 100 m non-usable wind speeds for the wind-turbines over Scandinavia and selected land areas in Alaska, northern Russia and Canada. In contrast, non-usable wind speeds decrease over large parts of Eastern Siberia and in northern Alaska. Thus, our results indicate increased potential of the Arctic for the development and production of wind energy. Bias corrected and not corrected near-surface wind speed and WPD changes have been compared with each other. It has been found that both show the same sign of future change, but differ in magnitude of these changes. The role of sea-ice retreat and vegetation expansion in the Arctic in future on near-surface wind speed variability has been also assessed. Surface roughness through sea-ice and vegetation changes may significantly impact on WPD variability in the Arctic.
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| 5. |
- Doyle, Samuel H., et al.
(författare)
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Amplified melt and flow of the Greenland ice sheet driven by late-summer cyclonic rainfall
- 2015
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Ingår i: Nature Geoscience. - 1752-0894 .- 1752-0908. ; 8:8, s. 647-
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Tidskriftsartikel (refereegranskat)abstract
- Intense rainfall events significantly affect Alpine and Alaskan glaciers through enhanced melting, ice-flow acceleration and subglacial sediment erosion, yet their impact on the Greenland ice sheet has not been assessed. Here we present measurements of ice velocity, subglacial water pressure and meteorological variables from the western margin of the Greenland ice sheet during a week of warm, wet cyclonic weather in late August and early September 2011. We find that extreme surface runoff from melt and rainfall led to a widespread acceleration in ice flow that extended 140 km into the ice-sheet interior. We suggest that the late-season timing was critical in promoting rapid runoff across an extensive bare ice surface that overwhelmed a subglacial hydrological system in transition to a less-efficient winter mode. Reanalysis data reveal that similar cyclonic weather conditions prevailed across southern and western Greenland during this time, and we observe a corresponding ice-flow response at all land- and marine-terminating glaciers in these regions for which data are available. Given that the advection of warm, moist air masses and rainfall over Greenland is expected to become more frequent in the coming decades, our findings portend a previously unforeseen vulnerability of the Greenland ice sheet to climate change.
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| 6. |
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| 7. |
- Lee, Hanna, et al.
(författare)
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Toward Effective Collaborations between Regional Climate Modeling and Impacts-Relevant Modeling Studies in Polar Regions
- 2022
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Ingår i: Bulletin of the American Meteorological Society. - 0003-0007. ; 103:8, s. 1866-1874
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Tidskriftsartikel (refereegranskat)abstract
- The aim of this workshop was to discuss the needs and challenges in using high-resolution climate model outputs for impacts-relevant modeling. Development of impacts-relevant climate projections in the polar regions requires effective collaboration between regional climate modelers and impacts-relevant modelers in the design stage of high-resolution climate projections for the polar regions.
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| 8. |
- Vandecrux, Baptiste, et al.
(författare)
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The firn meltwater Retention Model Intercomparison Project (RetMIP) : evaluation of nine firn models at four weather station sites on the Greenland ice sheet
- 2020
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Ingår i: The Cryosphere. - : Copernicus GmbH. - 1994-0416 .- 1994-0424. ; 14:11, s. 3785-3810
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Tidskriftsartikel (refereegranskat)abstract
- Perennial snow, or firn, covers 80 % of the Greenland ice sheet and has the capacity to retain surface meltwater, influencing the ice sheet mass balance and contribution to sea-level rise. Multilayer firn models are traditionally used to simulate firn processes and estimate meltwater retention. We present, intercompare and evaluate outputs from nine firn models at four sites that represent the ice sheet's dry snow, percolation, ice slab and firn aquifer areas. The models are forced by mass and energy fluxes derived from automatic weather stations and compared to firn density, temperature and meltwater percolation depth observations. Models agree relatively well at the dry-snow site while elsewhere their meltwater infiltration schemes lead to marked differences in simulated firn characteristics. Models accounting for deep meltwater percolation overestimate percolation depth and firn temperature at the percolation and ice slab sites but accurately simulate recharge of the firn aquifer. Models using Darcy's law and bucket schemes compare favorably to observed firn temperature and meltwater percolation depth at the percolation site, but only the Darcy models accurately simulate firn temperature and percolation at the ice slab site. Despite good performance at certain locations, no single model currently simulates meltwater infiltration adequately at all sites. The model spread in estimated meltwater retention and runoff increases with increasing meltwater input. The highest runoff was calculated at the KAN_U site in 2012, when average total runoff across models (+/- 2 sigma) was 353 +/- 610 mm w. e. (water equivalent), about 27 +/- 48 % of the surface meltwater input. We identify potential causes for the model spread and the mismatch with observations and provide recommendations for future model development and firn investigation.
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