| 1. |
- Schiller, D, et al.
(author)
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The Human Affectome
- 2024
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In: Neuroscience and biobehavioral reviews. - 1873-7528. ; 158, s. 105450-
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Journal article (peer-reviewed)
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| 2. |
- Shrestha, R, et al.
(author)
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Molecular pathogenesis of progression to myeloid leukemia from TET-insufficient status
- 2020
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In: Blood advances. - : American Society of Hematology. - 2473-9537 .- 2473-9529. ; 4:5, s. 845-854
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Journal article (peer-reviewed)abstract
- Loss-of-function mutations in ten-eleven translocation-2 (TET2) are recurrent events in acute myeloid leukemia (AML) as well as in preleukemic hematopoietic stem cells (HSCs) of age-related clonal hematopoiesis. TET3 mutations are infrequent in AML, but the level of TET3 expression in HSCs has been found to decline with age. We examined the impact of gradual decrease of TET function in AML development by generating mice with Tet deficiency at various degrees. Tet2f/f and Tet3f/f mice were crossed with mice expressing Mx1-Cre to generate Tet2f/wtTet3f/fMx-Cre+ (T2ΔT3), Tet2f/fTet3f/wtMx-Cre+ (ΔT2T3), and Tet2f/fTet3f/fMx-Cre+ (ΔT2ΔT3) mice. All ΔT2ΔT3 mice died of aggressive AML at a median survival of 10.7 weeks. By comparison, T2ΔT3 and ΔT2T3 mice developed AML at longer latencies, with a median survival of ∼27 weeks. Remarkably, all 9 T2ΔT3 and 8 ΔT2T3 mice with AML showed inactivation of the remaining nontargeted Tet2 or Tet3 allele, respectively, owing to exonic loss in either gene or stop-gain mutations in Tet3. Recurrent mutations other than Tet3 were not noted in any mice by whole-exome sequencing. Spontaneous inactivation of residual Tet2 or Tet3 alleles is a recurrent genetic event during the development of AML with Tet insufficiency.
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| 3. |
- Sullivan, R. M., et al.
(author)
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Ontogenesis Of Time Interval Encoding In Odor Fear Conditioning
- 2012
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In: Developmental Psychobiology. - : John Wiley & Sons. - 0012-1630 .- 1098-2302. ; 54:7, s. 768-768
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Journal article (peer-reviewed)abstract
- In Pavlovian fear conditioning, an initially neutral stimulus predicts the arrival of an aversive stimulus at a fixed time interval. Accumulating evidence indicates that in associative learning temporal relations between events are encoded. However the neural networks underlying the encoding and memory of interval durations are poorly understood. We addressed this question in odor fear conditioning which can be performed in young pups as well as in adult animals. In this task, an odor is presented to the animal and after a fixed interval (30 seconds) a mild footshock is applied. We first investigated the ontogenesis of interval timing at the behavioral level. We designed an experimental setup allowing the simultaneous recording of respiration, ultrasonic vocalizations (USVs) and freezing in rats. These parameters greatly improved the sensitivity of fear behavior assessment, thus increasing the probability of detecting transient anticipatory fear responses. Three ages were considered: 14-days-old (infants), 22-days-old (juveniles) and 75-days-old (adults). The data show that in adults, an anticipator y response develops after a few odor-shock pairings, characterized by a decrease in respiratory rhythm and an increase in USVs emission prior to shock delivery. Similar anticipatory responses might be present in juveniles, while infants might show no signs of interval timing, despite showing good learning of the odor-shock association. In parallel, current experiments are investigating learning-induced brain activation at these three ages, using 2 Deoxyglucose metabolic mapping. We are focusing on structures known to be involved in timing in adults: striatum, nucleus accumbens and prefrontal cortex.
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| 4. |
- Tosh, J., et al.
(author)
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Evolutionary history of the Afro-Madagascan Ixora species (Rubiaceae) : species diversification and distribution of key morphological traits inferred from dated molecular phylogenetic trees
- 2013
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In: Annals of Botany. - : Oxford University Press (OUP). - 0305-7364 .- 1095-8290. ; 112:9, s. 1723-1742
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Journal article (peer-reviewed)abstract
- Previous work on the pantropical genus Ixora has revealed an Afro-Madagascan clade, but as yet no study has focused in detail on the evolutionary history and morphological trends in this group. Here the evolutionary history of Afro-Madagascan Ixora spp. (a clade of approx. 80 taxa) is investigated and the phylogenetic trees compared with several key morphological traits in taxa occurring in Madagascar. Phylogenetic relationships of Afro-Madagascan Ixora are assessed using sequence data from four plastid regions (petD, rps16, rpoB-trnC and trnL-trnF) and nuclear ribosomal external transcribed spacer (ETS) and internal transcribed spacer (ITS) regions. The phylogenetic distribution of key morphological characters is assessed. Bayesian inference (implemented in BEAST) is used to estimate the temporal origin of Ixora based on fossil evidence. Two separate lineages of Madagascan taxa are recovered, one of which is nested in a group of East African taxa. Divergence in Ixora is estimated to have commenced during the mid Miocene, with extensive cladogenesis occurring in the Afro-Madagascan clade during the Pliocene onwards. Both lineages of Madagascan Ixora exhibit morphological innovations that are rare throughout the rest of the genus, including a trend towards pauciflorous inflorescences and a trend towards extreme corolla tube length, suggesting that the same ecological and selective pressures are acting upon taxa from both Madagascan lineages. Novel ecological opportunities resulting from climate-induced habitat fragmentation and corolla tube length diversification are likely to have facilitated species radiation on Madagascar.
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