| 1. |
- Fischer, Benjamin, et al.
(författare)
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A complete workflow for the differentiation and the dissociation of hiPSC-derived cardiospheres
- 2018
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Ingår i: Stem Cell Research. - : Elsevier. - 1873-5061 .- 1876-7753. ; 32, s. 65-72
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Tidskriftsartikel (refereegranskat)abstract
- Cardiomyocytes derived from human induced pluripotent stem cells (hiPSC-CMs) are an invaluable tool for both basic and translational cardiovascular research. The potential that these cells hold for therapy, disease modeling and drug discovery is hampered by several bottlenecks that currently limit both the yield and the efficiency of cardiac induction. Here, we present a complete workflow for the production of ready-to-use hiPSC-CMs in a dynamic suspension bioreactor. This includes the efficient and highly reproducible differentiation of hiPSCs into cardiospheres, which display enhanced physiological maturation compared to static 3D induction in hanging drops, and a novel papain-based dissociation method that offers higher yield and viability than the broadly used dissociation reagents TrypLE and Accutase. Molecular and functional analyses of the cardiomyocytes reseeded after dissociation confirmed both the identity and the functionality of the cells, which can be used in down-stream applications, either as monolayers or spheroids.
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| 2. |
- Neumann, Jonas, et al.
(författare)
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Recycling of Lithium-Ion Batteries—Current State of the Art, Circular Economy, and Next Generation Recycling
- 2022
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Ingår i: Advanced Energy Materials. - : Wiley. - 1614-6840 .- 1614-6832. ; 12:17
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Forskningsöversikt (refereegranskat)abstract
- Being successfully introduced into the market only 30 years ago, lithium-ion batteries have become state-of-the-art power sources for portable electronic devices and the most promising candidate for energy storage in stationary or electric vehicle applications. This widespread use in a multitude of industrial and private applications leads to the need for recycling and reutilization of their constituent components. Improving the “recycling technology” of lithium ion batteries is a continuous effort and recycling is far from maturity today. The complexity of lithium ion batteries with varying active and inactive material chemistries interferes with the desire to establish one robust recycling procedure for all kinds of lithium ion batteries. Therefore, the current state of the art needs to be analyzed, improved, and adapted for the coming cell chemistries and components. This paper provides an overview of regulations and new battery directive demands. It covers current practices in material collection, sorting, transportation, handling, and recycling. Future generations of batteries will further increase the diversity of cell chemistry and components. Therefore, this paper presents predictions related to the challenges of future battery recycling with regard to battery materials and chemical composition, and discusses future approaches to battery recycling.
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| 3. |
- Wylensek, David, et al.
(författare)
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A collection of bacterial isolates from the pig intestine reveals functional and taxonomic diversity
- 2020
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Ingår i: Nature Communications. - : Nature Publishing Group. - 2041-1723. ; 11:1
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Tidskriftsartikel (refereegranskat)abstract
- Our knowledge about the gut microbiota of pigs is still scarce, despite the importance of these animals for biomedical research and agriculture. Here, we present a collection of cultured bacteria from the pig gut, including 110 species across 40 families and nine phyla. We provide taxonomic descriptions for 22 novel species and 16 genera. Meta-analysis of 16S rRNA amplicon sequence data and metagenome-assembled genomes reveal prevalent and pig-specific species within Lactobacillus, Streptococcus, Clostridium, Desulfovibrio, Enterococcus, Fusobacterium, and several new genera described in this study. Potentially interesting functions discovered in these organisms include a fucosyltransferase encoded in the genome of the novel species Clostridium porci, and prevalent gene clusters for biosynthesis of sactipeptide-like peptides. Many strains deconjugate primary bile acids in in vitro assays, and a Clostridium scindens strain produces secondary bile acids via dehydroxylation. In addition, cells of the novel species Bullifex porci are coccoidal or spherical under the culture conditions tested, in contrast with the usual helical shape of other members of the family Spirochaetaceae. The strain collection, called 'Pig intestinal bacterial collection' (PiBAC), is publicly available at www.dsmz.de/pibac and opens new avenues for functional studies of the pig gut microbiota. The authors present a public collection of 117 bacterial isolates from the pig gut, including the description of 38 novel taxa. Interesting functions discovered in these organisms include a new fucosyltransferease and sactipeptide-like molecules encoded by biosynthetic gene clusters.
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