| 1. |
- Beal, Jacob, et al.
(författare)
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Robust estimation of bacterial cell count from optical density
- 2020
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Ingår i: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
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Tidskriftsartikel (refereegranskat)abstract
- Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
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| 2. |
- Kristanl, Matej, et al.
(författare)
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The Seventh Visual Object Tracking VOT2019 Challenge Results
- 2019
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Ingår i: 2019 IEEE/CVF INTERNATIONAL CONFERENCE ON COMPUTER VISION WORKSHOPS (ICCVW). - : IEEE COMPUTER SOC. - 9781728150239 ; , s. 2206-2241
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Konferensbidrag (refereegranskat)abstract
- The Visual Object Tracking challenge VOT2019 is the seventh annual tracker benchmarking activity organized by the VOT initiative. Results of 81 trackers are presented; many are state-of-the-art trackers published at major computer vision conferences or in journals in the recent years. The evaluation included the standard VOT and other popular methodologies for short-term tracking analysis as well as the standard VOT methodology for long-term tracking analysis. The VOT2019 challenge was composed of five challenges focusing on different tracking domains: (i) VOT-ST2019 challenge focused on short-term tracking in RGB, (ii) VOT-RT2019 challenge focused on "real-time" short-term tracking in RGB, (iii) VOT-LT2019 focused on long-term tracking namely coping with target disappearance and reappearance. Two new challenges have been introduced: (iv) VOT-RGBT2019 challenge focused on short-term tracking in RGB and thermal imagery and (v) VOT-RGBD2019 challenge focused on long-term tracking in RGB and depth imagery. The VOT-ST2019, VOT-RT2019 and VOT-LT2019 datasets were refreshed while new datasets were introduced for VOT-RGBT2019 and VOT-RGBD2019. The VOT toolkit has been updated to support both standard short-term, long-term tracking and tracking with multi-channel imagery. Performance of the tested trackers typically by far exceeds standard baselines. The source code for most of the trackers is publicly available from the VOT page. The dataset, the evaluation kit and the results are publicly available at the challenge website(1).
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| 3. |
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- 2019
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Tidskriftsartikel (refereegranskat)
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| 4. |
- Chen, Zheng wei, et al.
(författare)
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Reducing the aerodynamic drag of high-speed trains by air blowing from the nose part: Effect of blowing speed
- 2023
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Ingår i: Journal of Wind Engineering and Industrial Aerodynamics. - 0167-6105. ; 238
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Tidskriftsartikel (refereegranskat)abstract
- To reduce the aerodynamic drag of high-speed trains, this work proposes an air blowing configuration on the head and tail cars of high-speed trains. The variation in the aerodynamic drag and slipstream velocity is analyzed under different blowing velocities, and the flow mechanism for train aerodynamic performance alteration is explained. The results show that under the blowing speeds of Ub = 0.05Ut, 0.10Ut, 0.15Ut, and 0.20Ut, where Ut is the train speed, the total drag coefficient (Cd) decreases by 5.81%, 10.78%, 13.70%, and 15.43% compared to the without-blowing case, respectively. However, with the increase in the blowing speed, the reduction trend of Cd tends to be smoother; namely, the decrement ratio compared to the previous blowing speed for the head car is 9.08%, 0.11%, 0.60%, and 1.14% for Ub = 0.05Ut, 0.10Ut, 0.15Ut, and 0.20Ut, respectively. The blowing measure generates an air gap between the coming flow and train surface, consequently causing a reduction in the viscous and pressure drag. In addition, the structure size and strength of the wake flow under different blowing cases show a decreasing trend from Ub = 0.00Ut to 0.10Ut and then an increasing trend from Ub = 0.10Ut to 0.20Ut. Thus, considering the blowing cost, efficiency, and flow structure evolution comprehensively, the case of Ub = 0.10Ut is recommended. Under this blowing speed, the reduction ratio of the aerodynamic drag is 9.18%, 12.77%, 10.90%, and 10.78% for the head, middle, tail car, and total train, respectively.
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| 5. |
- Persson, Ninni, et al.
(författare)
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Age and sex related differences in subcortical brain iron concentrations among healthy adults
- 2015
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Ingår i: NeuroImage. - : Elsevier BV. - 1053-8119 .- 1095-9572. ; 122, s. 385-398
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Tidskriftsartikel (refereegranskat)abstract
- Age and sex can influence brain iron levels. We studied the influence of these variables on deep gray matter magnetic susceptibilities. In 183 healthy volunteers (44.7 ± 14.2 years, range 20–69, ♀ 49%), in vivo quantitative susceptibility mapping (QSM) at 1.5 T was performed to estimate brain iron accumulation in the following regions of interest (ROIs): caudate nucleus (Cd), putamen (Pt), globus pallidus (Gp), thalamus (Th), pulvinar (Pul), red nucleus (Rn), substantia nigra (Sn) and the cerebellar dentate nuclei (Dn). We gauged the influence of age and sex on magnetic susceptibility by specifying a series of structural equation models. The distributions of susceptibility varied in degree across the structures, conforming to histologic findings (Hallgren and Sourander, 1958), with the highest degree of susceptibility in the Gp and the lowest in the Th. Iron increase correlated across several ROIs, which may reflect an underlying age-related process. Advanced age was associated with a particularly strong linear rise of susceptibility in the striatum. Nonlinear age trends were found in the Rn, where they were the most pronounced, followed by the Pul and Sn, while minimal nonlinear trends were observed for the Pt, Th, and Dn. Moreover, sex related variations were observed, so that women showed lower levels of susceptibility in the Sn after accounting for age. Regional susceptibility of the Pul increased linearly with age in men but exhibited a nonlinear association with age in women with a leveling off starting from midlife. Women expected to be post menopause (+ 51 years) showed lower total magnetic susceptibility in the subcortical gray matter. The current report not only is consistent with previous reports of age related variations of brain iron, but also adds to the current knowledge by reporting age-related changes in less studied, smaller subcortical nuclei. This is the first in-vivo report to show lower total subcortical brain iron levels selectively in women from midlife, compared to men and younger women. These results encourage further assessment of sex differences in brain iron. We anticipate that age and sex are important co-factors to take into account when establishing a baseline level for differentiating pathologic neurodegeneration from healthy aging. The variations in regional susceptibility reported herein should be evaluated further using a longitudinal study design to determine within-person changes in aging.
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