| 1. |
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| 2. |
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| 3. |
- Nyström, Ingela, et al.
(författare)
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Virtual Cranio-Maxillofacial Surgery Planning with Stereo Graphics and Haptics
- 2016
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Ingår i: Computer-Assisted Musculoskeletal Surgery. - Cham : Springer International Publishing. - 9783319129433 ; , s. 29-42
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Bokkapitel (refereegranskat)abstract
- Computer-Assisted Surgery (CAS) is a new tool for performing complex procedures in a predictable and safe way. This book is designed to serve as a comprehensive review of Computer-Assisted Surgery, covering the current status of both research and applications.CAS includes Virtual Preoperative Planning (VPP) and Intraoperative Virtual Navigation (IVN), which are a set of technologies used to measure oncological margins in 3-Dimensions (3D), to locate small intraosseous tumors and apply controlled resections preserving anatomical structures. During VPP, patient acquired multimodal images are processed and an interactive virtual scenario is created. This can then be used as a platform to measure oncological distances and preplan osteotomies in safe areas. IVN is a procedure which allows the execution of the VPP with a mean error of less than 3mm.For the student, medical doctors, research and development scientists or new researchers, the protocols are central to the performance of Computer-Assisted technologies.
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| 4. |
- Svensson, Lennart, et al.
(författare)
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ProViz : a tool for explorative 3-D visualization and template matching in electron tomograms
- 2017
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Ingår i: Computer Methods in Biomechanics and Biomedical Engineering: Imaging & Visualization. - : Taylor & Francis. - 2168-1163 .- 2168-1171. ; 5:6, s. 446-454
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Tidskriftsartikel (refereegranskat)abstract
- Visual understanding is a key aspect when studying electron tomography data-sets, aside quantitative assessments such as registration of high-resolution structures. We here present the free software tool ProViz (Protein Visualization) for visualisation and template matching in electron tomograms of biological samples. The ProViz software contains methods and tools which we have developed, adapted and computationally optimised for easy and intuitive visualisation and analysis of electron tomograms with low signal-to-noise ratio. ProViz complements existing software in the application field and serves as an easy and convenient tool for a first assessment and screening of the tomograms. It provides enhancements in three areas: (1) improved visualisation that makes connections as well as intensity differences between and within objects or structures easier to see and interpret, (2) interactive transfer function editing with direct visual result feedback using both piecewise linear functions and Gaussian function elements, (3) computationally optimised template matching and tools to visually assess and interactively explore the correlation results. The visualisation capabilities and features of ProViz are demonstrated on various biological volume data-sets: bacterial filament structures in vitro, a desmosome and the transmembrane cadherin connections therein in situ, and liposomes filled with doxorubicin in solution. The explorative template matching is demonstrated on a synthetic IgG data-set.
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| 5. |
- Banerjee, Subhashis, et al.
(författare)
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Streamlining neuroradiology workflow with AI for improved cerebrovascular structure monitoring
- 2024
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Ingår i: Scientific Reports. - : Nature Publishing Group. - 2045-2322. ; 14:1
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Tidskriftsartikel (refereegranskat)abstract
- Radiological imaging to examine intracranial blood vessels is critical for preoperative planning and postoperative follow-up. Automated segmentation of cerebrovascular anatomy from Time-Of-Flight Magnetic Resonance Angiography (TOF-MRA) can provide radiologists with a more detailed and precise view of these vessels. This paper introduces a domain generalized artificial intelligence (AI) solution for volumetric monitoring of cerebrovascular structures from multi-center MRAs. Our approach utilizes a multi-task deep convolutional neural network (CNN) with a topology-aware loss function to learn voxel-wise segmentation of the cerebrovascular tree. We use Decorrelation Loss to achieve domain regularization for the encoder network and auxiliary tasks to provide additional regularization and enable the encoder to learn higher-level intermediate representations for improved performance. We compare our method to six state-of-the-art 3D vessel segmentation methods using retrospective TOF-MRA datasets from multiple private and public data sources scanned at six hospitals, with and without vascular pathologies. The proposed model achieved the best scores in all the qualitative performance measures. Furthermore, we have developed an AI-assisted Graphical User Interface (GUI) based on our research to assist radiologists in their daily work and establish a more efficient work process that saves time.
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| 6. |
- Blache, Ludovic, et al.
(författare)
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SoftCut: : A Virtual Planning Tool for Soft Tissue Resection on CT Images
- 2018
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Ingår i: Medical Image Understanding and Analysis. - Cham : Springer. - 9783319959207 ; , s. 299-310
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Konferensbidrag (refereegranskat)abstract
- With the increasing use of three-dimensional (3D) models and Computer Aided Design (CAD) in the medical domain, virtual surgical planning is now frequently used. Most of the current solutions focus on bone surgical operations. However, for head and neck oncologic resection, soft tissue ablation and reconstruction are common operations. In this paper, we propose a method to provide a fast and efficient estimation of shape and dimensions of soft tissue resections. Our approach takes advantage of a simple sketch-based interface which allows the user to paint the contour of the resection on a patient specific 3D model reconstructed from a computed tomography (CT) scan. The volume is then virtually cut and carved following this pattern. From the outline of the resection defined on the skin surface as a closed curve, we can identify which areas of the skin are inside or outside this shape. We then use distance transforms to identify the soft tissue voxels which are closer from the inside of this shape. Thus, we can propagate the shape of the resection inside the soft tissue layers of the volume. We demonstrate the usefulness of the method on patient specific CT data.
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| 7. |
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| 8. |
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| 9. |
- Moafi, Roya, et al.
(författare)
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Haptic-Assisted Surgical Planning (HASP) in a Case of Bilateral Mandible Fracture
- 2022
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Ingår i: International Medical Case Reports Journal. - : Dove Medical Press Limited. - 1179-142X. ; 15, s. 707-712
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Tidskriftsartikel (refereegranskat)abstract
- Restoring normal skeletal anatomy in patients with complex trauma to the mandible can be difficult, the difficulty often increasing with an edentulous mandible. This study describes a case of a displaced edentulous bilateral mandibular fracture, which was preoperatively planned with the in-house haptic-assisted surgery planning system (HASP). A model of the virtually restored mandible was 3D-printed at the hospital and a reconstruction plate was outlined beforehand with the printed mandible as a template and served as a guide during surgery. This case suggests HASP as a valuable preoperative tool in the planning phase when dealing with maxillofacial trauma cases. With the application of virtual planning, the authors could analyze the desired outcome and were further supported in surgery by the guidance of the reconstruction plate outlined on the restored model of the mandible.
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| 10. |
- Nilsson, Johanna, et al.
(författare)
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Evaluation of in-house, haptic assisted surgical planning for virtual reduction of complex mandibular fractures
- 2021
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Ingår i: International Journal of Computer Assisted Radiology and Surgery. - : Springer. - 1861-6410 .- 1861-6429. ; 16:6, s. 1059-1068
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Tidskriftsartikel (refereegranskat)abstract
- The management of complex mandible fractures, i.e severely comminuted or fractures of edentulous/atrophic mandibles, can be challenging. This is due to the three-dimensional loss of bone, which limits the possibility for accurate anatomic reduction. Virtual surgery planning (VSP) can provide improved accuracy and shorter operating times, but is often not employed for trauma cases because of time constraints and complex user interfaces limited to two-dimensional interaction with three-dimensional data. In this study, we evaluate the accuracy, precision, and time efficiency of the Haptic Assisted Surgery Planning system (HASP), an in-house VSP system that supports stereo graphics, six degrees-of-freedom input and haptics, to improve the surgical planning. Three operators performed planning in HASP on Computed Tomography (CT) and Come Beam Computed Tomography (CBCT) images of a plastic skull model and on twelve retrospective cases with complex mandible fractures. The result shows an accuracy and reproducibility of less than 2mm when using HASP, with an average planning time of 15 minutes, including time for segmentation in the software BoneSplit. This study presents an in-house haptic assisted planning tool for cranio-maxillofacial surgery with high usability that can be used for preoperative planning and evaluation of complex mandible fractures.
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| 11. |
- Nysjö, Fredrik, 1985-
(författare)
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Clustered Grid Cell Data Structure for Isosurface Rendering
- 2020
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Ingår i: Journal of WSCG. - 1213-6972 .- 1213-6964. ; 28:1-2, s. 9-17
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Tidskriftsartikel (refereegranskat)abstract
- Active grid cells in scalar volume data are typically identified by many isosurface rendering methods when extracting another representation of the data for rendering. However, the use of grid cells themselves as rendering primitives is not extensively explored in the literature. In this paper, we propose a cluster-based data structure for storing the data of active grid cells for fast cell rasterisation via billboard splatting. Compared to previous cell rasterisation approaches, eight corner scalar values are stored with each active grid cell, so that the full volume data is not required during rendering. The grid cells can be quickly extracted and use about 37 percent memory compared to a typical efficient mesh-based representation, while supporting large grid sizes. We present further improvements such as a visibility buffer for cluster culling and EWA-based interpolation of attributes such as normals. We also show that our data structure can be used for hybrid ray tracing or path tracing to compute global illumination.
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| 12. |
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| 13. |
- Nysjö, Fredrik, 1985-
(författare)
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Modeling and Visualization for Virtual Interaction with Medical Image Data
- 2020
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Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- Interactive systems for exploring and analysing medical three dimensional (3D) volume image data using techniques such as stereoscopic rendering and haptics can lead to new workflows for virtual surgery planning. This includes the design of patient-specific surgical guides and plates for additive manufacturing (3D printing). Our applications, medical visualization and cranio-maxillofacial surgery planning, involve large volume data such as computed tomo\-graphy (CT) images with millions of data points. This motivates the development of fast and efficient methods for visualization and haptic rendering, as well as the development of efficient modeling techniques for simplifying the design of 3D printable parts. In this thesis, we develop methods for visualization and haptic rendering of isosurfaces in volume image data, and show applications of these methods to medical visualization and virtual surgery planning. We further develop methods for modeling surgical guides and plates for cranio-maxillofacial surgery, and integrate them into our system for haptics-assisted surgery planning called HASP. This system is now installed at the department of surgical sciences, Uppsala University, and is being evaluated for use in clinical research.
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| 14. |
- Nysjö, Fredrik, 1985-, et al.
(författare)
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RayCaching : Amortized Isosurface Rendering for Virtual Reality
- 2020
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Ingår i: Computer graphics forum (Print). - : Wiley. - 0167-7055 .- 1467-8659. ; 39:1, s. 220-230
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Tidskriftsartikel (refereegranskat)abstract
- Real‐time virtual reality requires efficient rendering methods to deal with high‐ resolution stereoscopic displays and low latency head‐tracking. Our proposed RayCaching method renders isosurfaces of large volume datasets by amortizing raycasting over several frames and caching primary rays as small bricks that can be efficiently rasterized. An occupancy map in form of a clipmap provides level of detail and ensures that only bricks corresponding to visible points on the isosurface are being cached and rendered. Hard shadows and ambient occlusion from secondary rays are also accumulated and stored in the cache. Our method supports real‐time isosurface rendering with dynamic isovalue and allows stereoscopic visualization and exploration of large volume datasets at framerates suitable for virtual reality applications.
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| 15. |
- Nysjö, Fredrik, 1985-, et al.
(författare)
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Signed Distance Fields for Modeling Surgical Guides and Plates from CT Images
- 2016
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Ingår i: Proc, Swedish Symposium on Image Analysis.
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Konferensbidrag (övrigt vetenskapligt/konstnärligt)abstract
- User-friendly virtual surgery planning(VSP) systems for reconstructive surgery, such as cranio-maxillofacial (CMF) surgery, that can be used by the surgeons themselves without help of a technician have the potential to shorten the pre-operative planning from days to hours. An important part of such systems is the design of surgical guides and plates for osteosynthesis. We describe a method for generating surgical guide and plate models from computed tomography (CT) images, using signed distance fields and constructive solidgeometry (CSG). We implement the method as an extension to our Haptics-Assisted Surgery Plan-ning (HASP) system that enables a user to quickly design guide and plate models with stereo graphics and haptic feedback. We find that surgical guide and plate models can be efficiently generated from segmented CT images with our method. We also find that an anti-aliased distance transform can improve the accuracy and precision of the modelingin this application.
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| 16. |
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| 17. |
- Nysjö, Fredrik, 1985-, et al.
(författare)
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Vectorised High-Fidelity Haptic Rendering with Dynamic Pointshell
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Annan publikation (övrigt vetenskapligt/konstnärligt)abstract
- Exploiting parallelism in haptic rendering algorithms for rigid body collision simulation can be difficult due to the haptic feedback loop imposing strict real-time constraints on the computations. In this paper, we show that the classical Voxmap PointShell algorithm can be efficiently vectorised via the single-program multiple-data (SPMD) programming model of the Intel SPMD Program Compiler (ISPC) compiler and programming language. Our vectorised version provides an average 3.0x speedup compared to a corresponding scalar implementation, for a static hierarchical pointshell on a single CPU core. In addition, we propose a dynamic pointshell that does not require any pre-processing and allows a fixed point budget to be set per frame. The speedup obtained by the vectorisation means that a larger number of contact queries can be processed per haptic frame, while maintaining a desired haptic framerate. In an empirical study, we demonstrate that this increased fidelity in collision simulation translates directly to a higher user accuracy in assembly of fractured virtual objects.
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| 18. |
- Olsson, Pontus, et al.
(författare)
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A haptics-assisted cranio-maxillofacial surgery planning system for restoring skeletal anatomy in complex trauma cases
- 2013
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Ingår i: International Journal of Computer Assisted Radiology and Surgery. - : Springer Science and Business Media LLC. - 1861-6410 .- 1861-6429. ; 8:6, s. 887-894
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Tidskriftsartikel (refereegranskat)abstract
- Cranio-maxillofacial (CMF) surgery to restore normal skeletal anatomy in patients with serious trauma to the face can be both complex and time-consuming. But it is generally accepted that careful pre-operative planning leads to a better outcome with a higher degree of function and reduced morbidity in addition to reduced time in the operating room. However, today's surgery planning systems are primitive, relying mostly on the user's ability to plan complex tasks with a two-dimensional graphical interface. A system for planning the restoration of skeletal anatomy in facial trauma patients using a virtual model derived from patient-specific CT data. The system combines stereo visualization with six degrees-of-freedom, high-fidelity haptic feedback that enables analysis, planning, and preoperative testing of alternative solutions for restoring bone fragments to their proper positions. The stereo display provides accurate visual spatial perception, and the haptics system provides intuitive haptic feedback when bone fragments are in contact as well as six degrees-of-freedom attraction forces for precise bone fragment alignment. A senior surgeon without prior experience of the system received 45 min of system training. Following the training session, he completed a virtual reconstruction in 22 min of a complex mandibular fracture with an adequately reduced result. Preliminary testing with one surgeon indicates that our surgery planning system, which combines stereo visualization with sophisticated haptics, has the potential to become a powerful tool for CMF surgery planning. With little training, it allows a surgeon to complete a complex plan in a short amount of time.
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| 19. |
- Olsson, Pontus, et al.
(författare)
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Comparison of walking and traveling-wave piezoelectric motors as actuators in kinesthetic haptic devices
- 2016
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Ingår i: IEEE Transactions on Haptics. - 1939-1412 .- 2329-4051. ; 9:3, s. 427-431
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Tidskriftsartikel (refereegranskat)abstract
- Piezoelectric motors offer an attractive alternative to electromagnetic actuators in portable haptic interfaces: they are compact, have a high force-to-volume ratio, and can operate with limited or no gearing. However, the choice of a piezoelectric motor type is not obvious due to differences in performance characteristics. We present our evaluation of two commercial, operationally different, piezoelectric motors acting as actuators in two kinesthetic haptic grippers, a walking quasi-static motor and a traveling wave ultrasonic motor. We evaluate each gripper's ability to display common virtual objects including springs, dampers, and rigid walls, and conclude that the walking quasi-static motor is superior at low velocities. However, for applications where high velocity is required, traveling wave ultrasonic motors are a better option.
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| 20. |
- Olsson, Pontus, et al.
(författare)
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Haptics-assisted Virtual Planning of Bone, Soft Tissue, and Vessels in Fibula Osteocutaneous Free Flaps
- 2015
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Ingår i: Plastic and Reconstructive Surgery - Global Open. - : Wolters Kluwer. - 2169-7574. ; 3:8
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Tidskriftsartikel (refereegranskat)abstract
- Background: Virtual surgery planning has proven useful for reconstructing head and neck defects by fibula osteocutaneous free flaps (FOFF). Benefits include improved healing, function, and aesthetics, as well as cost savings. But available virtual surgery planning systems incorporating fibula in craniomaxillofacial reconstruction simulate only bone reconstruction without considering vessels and soft tissue.Methods: The Haptics-Assisted Surgery Planning (HASP) system incorporates bone, vessels, and soft tissue of the FOFF in craniomaxillofacial defect reconstruction. Two surgeons tested HASP on 4 cases they had previously operated on: 3 with composite mandibular defects and 1 with a composite cervical spine defect. With the HASP stereographics and haptic feedback, using patient-specific computed tomography angiogram data, the surgeons planned the 4 cases, including bone resection, fibula design, recipient vessels selection, pedicle and perforator location selection, and skin paddle configuration.Results: Some problems encountered during the actual surgery could have been avoided as they became evident with HASP. In one case, the fibula reconstruction was incomplete because the fibula had to be reversed and thus did not reach the temporal fossa. In another case, the fibula had to be rotated 180 degrees to correct the plate and screw placement in relation to the perforator. In the spinal case, difficulty in finding the optimal fibula shape and position required extra ischemia time.Conclusions: The surgeons found HASP to be an efficient planning tool for FOFF reconstructions. The testing of alternative reconstructions to arrive at an optimal FOFF solution preoperatively potentially improves patient function and aesthetics and reduces operating room time.
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| 21. |
- Olsson, Pontus, 1980-, et al.
(författare)
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Physically Co-Located Haptic Interaction with 3D Displays
- 2012
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Ingår i: Proc. Haptics Symposium (HAPTICS), 2012 IEEE. - 9781467308083 ; , s. 267-272
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Konferensbidrag (refereegranskat)abstract
- Studies indicate that haptic interaction with a computer generated virtual scene may become more intuitive by aligning (co-locating) the visual and haptic workspaces so that the visual and haptic feedback coincide as they do in the real world. Co-located haptics may gain importance when more advanced haptic interfaces, such as high-fidelity whole hand devices, become available. We describe a user study that investigates the pros and cons with physically co-located versus non-collocated haptics on two different display types: a commercial half-transparent mirror 3D display with shutter glasses and a prototype autostereoscopic display based on a Holographic Optical Element (HOE). We use two accuracy tasks with spatial accuracy as the dependent variable and one manipulation task with time as the dependent variable. The study shows that on both displays co-location significantly improves completion time in the manipulation task. However, the study shows that co-location does not improve the accuracy in the spatial accuracy tasks.
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| 22. |
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| 23. |
- Olsson, Pontus, 1980-, et al.
(författare)
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Snap-to-fit, a Haptic 6 DOF Alignment Tool for Virtual Assembly
- 2013
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Ingår i: Proc. World Haptics (WHC), 2013 IEEE. - 9781479900879 ; , s. 205-210
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Konferensbidrag (refereegranskat)abstract
- Virtual assembly of complex objects has application in domains ranging from surgery planning to archaeology. In these domains the objective is to plan the restoration of skeletal anatomy or archaeological artifacts to achieve an optimal reconstruction without causing further damage. While graphical modeling plays a central role in virtual assembly, visual feedback alone is often insufficient since object contact and penetration is difficult to discern due to occlusion. Haptics can improve an assembly task by giving feedback when objects collide, but precise fitting of fractured objects guided by delicate haptic cues similar to those present in the physical world requires haptic display transparency beyond the performance of today’s systems. We propose a haptic alignment tool that combines a 6 Degrees of Freedom (DOF) attraction force with traditional 6 DOF contact forces to pull a virtual object towards a local stable fit with a fixed object. The object forces are integrated into a virtual coupling framework yielding a stable haptic tool. We demonstrate the use of our system on applications from both cranio-maxillofacial surgery and archaeology, and show that we can achieve haptic rates for fractured surfaces with over 5000 points.
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| 24. |
- Olsson, Pontus, 1980-, et al.
(författare)
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SplineGrip - An Eight Degrees-of-Freedom Flexible Haptic Sculpting Tool
- 2013
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Ingår i: ACM SIGGRAPH 2013 Posters. - New York, NY, USA : ACM.
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Konferensbidrag (refereegranskat)abstract
- SplineGrip is a flexible haptic sculpting tool that senses the articulation and pose (position and orientation) of the sculpting hand in eight degrees-of-freedom (DOF). The tool captures the hand articulation in two DOF, and uses a commercial haptic device to track the hand pose in six DOF and to simultaneously provide three DOF haptic feedback. The eight DOF input is mapped to the pose and shape of a virtual NURBS-based sculpting tool, offering versatile interaction with a virtual model. We capture the hand articulation in two DOF using two bend sensors with curvature dependent resistance, which are attached in two directions to a flexible plastic sheet mounted on the gimbal of the haptic device. One sensor measures the plastic sheet curvature controlled by the thumb, and the other measures the curvature controlled by the middle and ring fingers. In a neutral state, when all fingers are straight, the virtual sculpting tool takes the shape of a line segment. By bending one sensor with the middle and ring fingers, the user changes the virtual tool curvature. By bending the other sensor with the thumb, the user changes the width of the virtual tool. A curvature increase at zero width turns the line into a spline, and a width increase at zero curvature creates a plane. By bending both sensors, the user may simultaneously control the curvature and width of the NURBS surface. The user may toggle between negative and positive curvatures to make convex and concave tools. We demonstrate SplineGrip with a simple sculpting system where the user starts with a block of material and uses the virtual sculpting tool to gradually remove material; the sculpting tool is not limited to subtractive modeling, but can work with other modeling paradigms.
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| 25. |
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| 26. |
- Olsson, Pontus, 1980-, et al.
(författare)
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Whole Hand Haptics
- 2011
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Ingår i: Medicinteknikdagarna Oktober 11-12 2011, Linköping, Sweden.
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Konferensbidrag (refereegranskat)abstract
- Our sense of touch is in many ways the most sophisticated of our senses with receptors throughout the body. Unlike vision and hearing, haptics provides bi-directional communication between an individual and his/her environment. Yet, our sense of touch has been exploited for computer interfaces mostly in primitive ways, with both input and output limited to contact with a single point on a virtual object or to signal an event. But a single point of contact is often insufficient for exploration and manipulation: try to pick up a small object with only one finger! Current multi-point interaction devices are built using mechanical tendons, which are large and bulky and provide neither the stiffness nor the dynamic range required for object manipulation. We present a first generation of a haptic glove that acts as an external skeleton where the hand and finger joints are controlled by actuators that are integrated in the glove. This first prototype allows for six degrees of freedom (DOF) movement of the hand, and one DOF gripping with the thumb and index finger. The six DOF movements are accomplished with a commercial haptic arm, which allows us to simulate physical object properties such as weight, friction and inertia. The gripping force is controlled by the most compact high precision piezoelectric motor that is commercially available today, using a separate force sensor in a feed-back loop. The high stiffness of the motor in combination with a high dynamic speed range allows for delicate control of the gripping force. Combined with emerging 3D display technology, the haptic glove opens up exciting possibilities of co-located visio-haptic interaction more closely resembling real-world interaction.
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| 27. |
- Pielawski, Nicolas, et al.
(författare)
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TissUUmaps 3 : Improvements in interactive visualization, exploration, and quality assessment of large-scale spatial omics data
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Annan publikation (övrigt vetenskapligt/konstnärligt)abstract
- Background and Objectives: Spatially resolved techniques for exploring the molecular landscape of tissue samples, such as spatial transcriptomics, often result in millions of data points and images too large to view on a regular desktop computer, limiting the possibilities in visual interactive data exploration. TissUUmaps is a free, open-source browser-based tool for GPU-accelerated visualization and interactive exploration of 107+ data points overlaying tissue samples.Methods: Herein we describe how TissUUmaps 3 provides instant multiresolution image viewing and can be customized, shared, and also integrated into Jupyter Notebooks. We introduce new modules where users can visualize markers and regions, explore spatial statistics, perform quantitative analyses of tissue morphology, and assess the quality of decoding in situ transcriptomics data.Results: We show that thanks to targeted optimizations the time and cost associated with interactive data exploration were reduced, enabling TissUUmaps 3 to handle the scale of today’s spatial transcriptomics methods.Conclusion: TissUUmaps 3 provides significantly improved performance for large multiplex datasets as compared to previous versions. We envision TissUUmaps to contribute to broader dissemination and flexible sharing of large-scale spatial omics data.
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| 28. |
- Pielawski, Nicolas, et al.
(författare)
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TissUUmaps 3 : Improvements in interactive visualization, exploration, and quality assessment of large-scale spatial omics data
- 2023
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Ingår i: Heliyon. - : Elsevier BV. - 2405-8440. ; 9:5
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Tidskriftsartikel (refereegranskat)abstract
- Background and objectives: Spatially resolved techniques for exploring the molecular landscape of tissue samples, such as spatial transcriptomics, often result in millions of data points and images too large to view on a regular desktop computer, limiting the possibilities in visual interactive data exploration. TissUUmaps is a free, open-source browser-based tool for GPU-accelerated visualization and interactive exploration of 107+ data points overlaying tissue samples.Methods: Herein we describe how TissUUmaps 3 provides instant multiresolution image viewing and can be customized, shared, and also integrated into Jupyter Notebooks. We introduce new modules where users can visualize markers and regions, explore spatial statistics, perform quantitative analyses of tissue morphology, and assess the quality of decoding in situ transcriptomics data.Results: We show that thanks to targeted optimizations the time and cost associated with interactive data exploration were reduced, enabling TissUUmaps 3 to handle the scale of today's spatial transcriptomics methods.Conclusion: TissUUmaps 3 provides significantly improved performance for large multiplex datasets as compared to previous versions. We envision TissUUmaps to contribute to broader dissemination and flexible sharing of largescale spatial omics data.
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