| 1. |
|
|
| 2. |
- Forsvall, A., et al.
(författare)
-
An evaluation of the Rastreometro, a new device for populational screening for high blood pressure in developing countries
- 2006
-
Ingår i: Arq Bras Cardiol. - 1678-4170. ; 87:4, s. 480-6
-
Tidskriftsartikel (refereegranskat)abstract
- OBJECTIVE: To test a simplified blood pressure device called Rastreometro that could be used by the Health Agents. METHODS: The Rastreometro has been developed from an ordinary aneroid sphygmomanometer, in which the numeric display is covered by an adhesive with a red zone, indicating pressures equal or above 140 mmHg and a yellow zone indicating pressures below 140 mmHg. The onset of oscillations of the aneroid needle is taken as an indication of the systolic pressure value. The measurements made by the Rastreometro were compared with those made by the auscultatory method, and were carried out in 268 patients, by two operators. The influence on the results of confounding variables such as age, gender, BMI, arm length, upper arm circumference, skin colour and antihypertensive treatment were taken into consideration, as well as intra and interobserver variation. RESULTS: In the whole group, sensitivity was 95.1%, specificity was 63.1%, positive predictive value was 62.4% and negative predictive value was 95.3%. Hypertensive treatment significantly affected specificity, 32.7% as compared to 77.8% for the non-treated group. Both operators improved their results over time. CONCLUSION: This study suggests that the Rastreometro technique, as a screener for hypertension, has good sensitivity. Concerning specificity, it is acceptable, provided the patient is not on regular antihypertensive treatment. In this latter situation, it can be improved by a proper standardization of the method to read the systolic pressure by needle oscillations. Furthermore, the use of this technique requires well trained operators.
|
|
| 3. |
- Hammar, M., et al.
(författare)
-
1300-nm GaAs-based vertical-cavity lasers
- 2002
-
Ingår i: Proceedings of SPIE - The International Society for Optical Engineering. - Brugge : SPIE. ; , s. 137-149
-
Konferensbidrag (refereegranskat)abstract
- We compare GaInNAs and highly strained InGaAs quantum-wells (QWs) for applications in metal-organic vapor-phase epitaxy (MOVPE)-grown GaAs-based 1300-nm vertical-cavity lasers (VCLs). While the peak wavelength of InGaAs QWs can be extended by a small fraction of N, the luminescence efficiency degrades strongly with wavelength. On the other hand, using highly strained InGaAs QWs in combination with a large VCL detuning it is also possible to push the emission wavelength towards 1.3 ÎŒm. The optimized MOVPE growth conditions for such QW and VCL structures are discussed in some detail. It is noted that GaInNAs and InGaAs QWs preferably are grown at low temperature, but with quite different V/III ratios and growth rates. We also point out the importance of reduced doping concentration and growth temperature of the n-doped bottom distributed Bragg reflector to minimize optical loss and for compatibility with GaInNAs QWs. InGaAs VCLs with emission wavelengths beyond 1260 nm are demonstrated. This includes mW-range output power, mA-range threshold current and 10 Gb/s data transmission.
|
|
| 4. |
- Hoiseth, G., et al.
(författare)
-
Evaluating the hip-flask defence using analytical data from ethanol and ethyl glucuronide. A comparison of two models
- 2020
-
Ingår i: Forensic Science International. - : ELSEVIER IRELAND LTD. - 0379-0738 .- 1872-6283. ; 316
-
Tidskriftsartikel (refereegranskat)abstract
- Aim: Claimed intake of alcohol after a traffic incident, called the hip-flask defence, can be objectively assessed by different methods. One of them is the use of two consecutive ethanol concentrations in urine and the ratio between ethanol concentrations in urine and blood. Another one is the concentrations of ethyl glucuronide (EtG) and ethyl sulphate (EtS) in blood and their ratio to ethanol. The experimental basis for both these models is from single dose studies only. The aim of this study was therefore to describe the kinetics of ethanol, EtG and EtS after ingestion of two repeated doses of ethanol and to investigate the usefulness of the different models for the assessment of the hip-flask defence. Methods: Thirty-five subjects ingested a first dose of 0.51 g of ethanol per kilo body weight, and two hours later a second dose (the hip-flask drink) of 0.25, 0.51 or 0.85 g of ethanol per kilo body weight. Ten urine and 17 blood samples were collected and analysed for ethanol, EtG and EtS using fully validated methods. It was investigated if all subjects fulfilled the criteria for recent drinking, according to the two different models, when using the samples collected 180-240 minutes after start of first dose drinking. According to the first model, increase in urinary ethanol concentrations and a ratio UAC/BAC below 1.3 indicated recent drinking. According to the second model, increase in blood EtG concentrations and a ratio ethanol (g/kg)/EtG (mg/L) above 1 indicated recent drinking. Results: All subjects in the high dose group fulfilled all criteria for recent drinking. One subject in the medium dose group and nine subjects in the low dose group failed to show increasing UAC and/or a UAC/ BAC ratio below 1.3. One subject in the low dose group failed to show increasing concentrations of blood EtG, but all subjects showed a ratio ethanol/EtG above 1. Conclusions: The present study showed, by the use of experimental data, that both two models used to investigate the hip-flask defence can be used, but only when the hip-flask dose is sufficiently high. (C) 2020 The Author(s). Published by Elsevier B.V.
|
|
| 5. |
|
|
| 6. |
- Mogg, S., et al.
(författare)
-
1.3-ÎŒm InGaAs(N)/GaAs vertical-cavity lasers
- 2003
-
Ingår i: Proceedings of SPIE - The International Society for Optical Engineering. - San Jose, CA : SPIE. ; , s. 139-151
-
Konferensbidrag (refereegranskat)abstract
- In this work we present performance characteristics of metalorganic vapor-phase epitaxy grown GaInNAs and InGaAs quantum-well (QW) vertical-cavity lasers (VCLs) for 1.3-Όm applications. The InGaAs VCLs emit in a wavelength range from 1200 to somewhat above 1260 nm, while the GaInNAs VCLs operate from 1265 to 1303 nm. The InGaAs VCLs are based on highly strained InGaAs double QWs, with photoluminescence (PL) maximum at around 1190 nm, and extensive negative gain-cavity detuning. As a consequence, these devices are strongly temperature sensitive and the minimum threshold current is found at very high temperature (∌90-100°C). Both kind of VCLs work continuous-wave well above 100°C, and while the InGaAs VCLs reach slightly higher light output power, they show significantly larger threshold currents. In addition, the large device detuning also has profound effects on the high-frequency response. Nevertheless, for a 1260-nm device, 10 Gb/s transmission is demonstrated in a back-to-back configuration. We also show that by further optimization of the InGaAs QWs the PL peak wavelength can be extended to at least 1240 nm. The incorporation of such QWs in the present VCL structure should considerably improve the device performance, resulting in higher light output power, lower threshold current, and reduced temperature sensitivity with a shift of the minimum threshold current towards room temperature, thus approaching standard VCL tuning.
|
|
| 7. |
|
|
| 8. |
- Al-Khalili, Lubna, et al.
(författare)
-
Profiling of human myotubes reveals an intrinsic proteomic signature associated with type 2 diabetes
- 2014
-
Ingår i: Translational Proteomics. - : Elsevier BV. - 2212-9634 .- 2212-9626. ; 2:1, s. 25-38
-
Tidskriftsartikel (refereegranskat)abstract
- The development of insulin resistance and type 2 diabetes (T2D) involves a complex array of metabolic defects in skeletal muscle. An in vitro cell culture system excludes the acute effects of external systemic factors existing in vivo. Thus, we aimed to determine whether intrinsic differences in the protein profile exist in cultured myotubes derived from T2D versus normal glucose tolerant (NGT) healthy people. Applying two dimensional difference gel electrophoresis technology (2-D DIGE), the abundance of 47 proteins differed in myotubes derived from T2D patients versus NGT donors. Proteins involved in fatty acid and amino acid metabolism, TCA cycle, mitochondrial function, mRNA processing, DNA repair and cell survival showed higher abundance, while proteins associated with redox signaling (PARK7; Parkinson disease 7), glutathione metabolism (glutathione S-transferase, GST, isoforms T1, P1 and M2), and protein dynamics (heat shock protein, HSP, isoform B1 and 90A) showed reduced abundance in myotubes derived from T2D versus NGT donors. Consistent with our proteome analysis results, the level of total glutathione was reduced in myotubes obtained from T2D versus NGT donors. Taken together, our data provide evidence for intrinsic differences in the profile of proteins involved in energy metabolism, cellular oxidative stress, protein dynamics and gene regulation in myotubes derived from T2D patients. These differences thereby suggest a genetic or epigenetic influence on protein content level, which can be further investigated to understand the molecular underpinnings of T2D progression and lead to new therapeutic approaches.
|
|
| 9. |
- Asplund, Carl, et al.
(författare)
-
1260 nm InGaAs vertical-cavity lasers
- 2002
-
Ingår i: Electronics Letters. - : Institution of Engineering and Technology (IET). - 0013-5194 .- 1350-911X. ; 38:13, s. 635-636
-
Tidskriftsartikel (refereegranskat)abstract
- The fabrication and performance of highly strained double-quantum well InGaAs/GaAs vertical-cavity lasers with record-long emission wavelength of 1260 nm at room temperature is reported. Depending on device diameter, the minimum threshold current is in the low mA-regime while the maximum output power exceeds 1 mW. The devices work continuous-wave over a wide temperature range of at least 10-120degrees C.
|
|
| 10. |
- Bohlooly-Yeganeh, Mohammad, 1966, et al.
(författare)
-
Growth hormone overexpression in the central nervous system results in hyperphagia-induced obesity associated with insulin resistance and dyslipidemia.
- 2005
-
Ingår i: Diabetes. - 0012-1797 .- 1939-327X. ; 54:1, s. 51-62
-
Tidskriftsartikel (refereegranskat)abstract
- It is well known that peripherally administered growth hormone (GH) results in decreased body fat mass. However, GH-deficient patients increase their food intake when substituted with GH, suggesting that GH also has an appetite stimulating effect. Transgenic mice with an overexpression of bovine GH in the central nervous system (CNS) were created to investigate the role of GH in CNS. This study shows that overexpression of GH in the CNS differentiates the effect of GH on body fat mass from that on appetite. The transgenic mice were not GH-deficient but were obese and showed increased food intake as well as increased hypothalamic expression of agouti-related protein and neuropeptide Y. GH also had an acute effect on food intake following intracerebroventricular injection of C57BL/6 mice. The transgenic mice were severely hyperinsulinemic and showed a marked hyperplasia of the islets of Langerhans. In addition, the transgenic mice displayed alterations in serum lipid and lipoprotein levels and hepatic gene expression. In conclusion, GH overexpression in the CNS results in hyperphagia-induced obesity indicating a dual effect of GH with a central stimulation of appetite and a peripheral lipolytic effect.
|
|
| 11. |
|
|
| 12. |
- Buijs, J, et al.
(författare)
-
Inter- and intra-molecular migration of peptide amide hydrogens during electrospray ionization
- 2001
-
Ingår i: JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY. - : ELSEVIER SCIENCE INC. - 1044-0305. ; 12:4, s. 410-419
-
Tidskriftsartikel (refereegranskat)abstract
- The isotopic exchange of amide hydrogens in proteins in solution strongly depends on the surrounding protein structure, thereby allowing structural studies of proteins by mass spectrometry. However, during electrospray ionization (ESI), gas phase processe
|
|
| 13. |
|
|
| 14. |
|
|
| 15. |
|
|
| 16. |
- Frick, Fredrik, 1973, et al.
(författare)
-
Hepatic and adipose tissue depot-specific changes in lipid metabolism in Late-onset Obese (LOB) rats.
- 2008
-
Ingår i: Lipids. - : Wiley. - 0024-4201 .- 1558-9307. ; 43:4, s. 313-24
-
Tidskriftsartikel (refereegranskat)abstract
- Transgenic Late-onset OBesity (LOB) rats slowly develop a male-specific, autosomal dominant, obesity phenotype with a specific increase in peri-renal white adipose tissue (WAT) depot and preserved insulin sensitivity (Bains et al. in Endocrinology 145:2666-2679, 2004). To better understand the remarkable phenotype of these rats, the lipid metabolism was investigated in male LOB and non-transgenic (NT) littermates. Total plasma cholesterol (C) levels were normal but total plasma triacylglycerol (TAG) (2.8-fold) and hepatic TAG content (25%) was elevated in LOB males. Plasma VLDL-C and VLDL-TAG levels were higher while plasma apoB levels were 60% lower in LOB males. Increased hepatic TAG secretion explained the increased VLDL levels in LOB males. The hepatic gene expression of FAS, SCD-1, mitochondrial (mt)GPAT, and DGAT2 was up-regulated in both old obese and young non-obese LOB rats. Lipoprotein lipase (LPL) activity in heart and epididymal white adipose tissue (WAT) was unchanged, while LPL activity was increased in peri-renal WAT (30%) and decreased in soleus muscle (40%). Moreover, FAS, SCD-1 and DGAT2 gene expression was increased in peri-renal, but not in epididymal WAT. Basal lipolysis was reduced or unchanged and beta-adrenergic stimulated lipolysis was reduced in WAT from both old obese and young non-obese LOB rats. To summarize, the obese phenotype of LOB male rats is associated with increased hepatic TAG production and secretion, a shift in LPL activity from skeletal muscle to WAT, reduced lipolytic response in WAT depots and a specific increase in expression of genes responsible for fatty acid and TAG synthesis in the peri-renal depot.
|
|
| 17. |
- Frick, F, et al.
(författare)
-
Interaction between growth hormone and insulin in the regulation of lipoprotein metabolism in the rat
- 2002
-
Ingår i: American journal of physiology. Endocrinology and metabolism. - : American Physiological Society. - 0193-1849 .- 1522-1555. ; 283:5, s. E1023-E1031
-
Tidskriftsartikel (refereegranskat)abstract
- The importance of insulin for the in vivo effects of growth hormone (GH) on lipid and lipoprotein metabolism was investigated by examining the effects of GH treatment of hypophysectomized (Hx) female rats with and without concomitant insulin treatment. Hypophysectomy-induced changes of HDL, apolipoprotein (apo)E, LDL, and apoB levels were normalized by GH treatment but not affected by insulin treatment. The hepatic triglyceride secretion rate was lower in Hx rats than in normal rats and increased by GH treatment. This effect of GH was blunted by insulin treatment. The triglyceride content in the liver changed in parallel with the changes in triglyceride secretion rate, indicating that the effect of the hormones on triglyceride secretion was dependent on changed availability of triglycerides for VLDL assembly. GH and insulin independently increased editing of apoB mRNA, but the effects were not additive. The expression of fatty-acid synthase (FAS), stearoyl-CoA desaturase-1 (SCD-1), and sterol regulatory element-binding protein-1c (SREBP-1c) was increased by GH treatment. Insulin and GH had no additive effects on these genes; instead, insulin blunted the effect of GH on SREBP-1c mRNA. In contrast to the liver, adipose tissue expression of SREBP-1c, FAS, or SCD-1 mRNA was not influenced by GH. In conclusion, the increased hepatic expression of lipogenic enzymes after GH treatment may be explained by increased expression of SREBP-1c. Insulin does not mediate the effects of GH but inhibits the stimulatory effect of GH on hepatic SREBP-1c expression and triglyceride secretion rate.
|
|
| 18. |
- Goienetxea Uriarte, Ainhoa, 1983-, et al.
(författare)
-
Lean, Simulation and Optimization : A Win-Win combination
- 2016
-
Ingår i: Proceedings of the 2015 Winter Simulation Conference. - Piscataway, New Jersey : IEEE Computer Society. - 9781467397438 - 9781467397414 - 9781467397421 ; , s. 2227-2238
-
Konferensbidrag (refereegranskat)abstract
- Lean and simulation analysis are driven by the same objective, how to better design and improve processes making the companies more competitive. The adoption of lean has been widely spread in companies from public to private sectors and simulation is nowadays becoming more and more popular. Several authors have pointed out the benefits of combining simulation and lean, however, they are still rarely used together in practice. Optimization as an additional technique to this combination is even a more powerful approach especially when designing and improving complex processes with multiple conflicting objectives. This paper presents the mutual benefits that are gained when combining lean, simulation and optimization and how they overcome each other´s limitations. A framework including the three concepts, some of the barriers for its implementation and a real-world industrial example are also described.
|
|
| 19. |
- Grandahl, Maria, et al.
(författare)
-
A population based survey of school nurses' attitudes to the implemented HPV vaccination programme in Sweden
- 2015
-
Ingår i: Eurogin 2015. ; , s. 168-168
-
Konferensbidrag (refereegranskat)abstract
- Objective: To investigate school nurses’ attitudes to, and experiences of the school-based HPV vaccination programme,one year after its implementation in Sweden.Methods: Data were collected using a web-based questionnaire in spring 2013, and 83.1% (851/1024) of the nursesanswered the questionnaire.Results: The majority (88.9%, n=756) agreed that HPV vaccinations should be the school nurses’ responsibility, and mostalso agreed (81.5%, n=693) that boys also should be offered the vaccine. Two thirds, 66.9% (n=570), stated that they hadexperienced difficulties with the vaccination and of these 59.1% (n=337) considered the task time-consuming. Three outof four nurses, 76.1% (n=648), had been contacted by parents who raised questions regarding the vaccine. The most commonquestions were related to side effects. There were strong associations between the nurses’ received education aboutthe HPV vaccine and perceived knowledge about the HPV vaccine and a favourable attitude towards vaccination (both p<0.001). A school nurse with a high level of received education was 9.8 times more likely to have a positive attitude to HPVvaccination compared to a nurse with a low level of received education (p<0.001). Nurses with high perceived knowledgewere 2.5 times more likely to have a positive attitude compared to those with a low level of perceived knowledge(p=0.006).Conclusions: HPV vaccination is a complex and time-consuming task and the school nurses need adequate knowledge,education, skills and time in order to address questions and concerns from parents, as well as informing about HPV.
|
|
| 20. |
- Haney, Michael, et al.
(författare)
-
Scandinavian SSAI clinical practice guideline on choice of first-line vasopressor for patients with acute circulatory failure
- 2016
-
Ingår i: Acta Anaesthesiologica Scandinavica. - : WILEY-BLACKWELL. - 0001-5172 .- 1399-6576. ; 60:10, s. 1347-1366
-
Forskningsöversikt (refereegranskat)abstract
- BackgroundAdult critically ill patients often suffer from acute circulatory failure, necessitating use of vasopressor therapy. The aim of the Scandinavian Society of Anaesthesiology and Intensive Care Medicine (SSAI) task force for Acute Circulatory Failure was to present clinically relevant, evidence-based treatment recommendations on this topic. MethodsThis guideline was developed according to standards for trustworthy guidelines, including a systematic review of the literature and use of the GRADE methodology for assessment of the quality of evidence and for moving from evidence to recommendations. We assessed the following subpopulations of patients with acute circulatory failure: 1) shock in general, 2) septic shock, 3) cardiogenic shock, 4) hypovolemic shock and 5) other types of shock, including vasodilatory shock. We assessed patient-important outcome measures, including mortality, serious adverse reactions and quality-of-life. ResultsFor patients with shock in general and those with septic shock, we recommend using norepinephrine rather than dopamine, and we suggest using norepinephrine rather than epinephrine, vasopressin analogues, and phenylephrine. For patients with cardiogenic shock and those with hypovolemic shock, we suggest using norepinephrine rather than dopamine, and we provide no recommendations/suggestions of norepinephrine vs. epinephrine, vasopressin analogues, and phenylephrine. For patients with other types of shock, including vasodilatory shock, we suggest using norepinephrine rather than dopamine, epinephrine, vasopressin analogues, and phenylephrine. ConclusionsWe recommend using norepinephrine rather than other vasopressors as first-line treatment for the majority of adult critically ill patients with acute circulatory failure.
|
|
| 21. |
- Karched, Maribasappa, et al.
(författare)
-
Vesicle-independent extracellular release of a proinflammatory outer membrane lipoprotein in free-soluble form
- 2008
-
Ingår i: BMC Microbiology. - : Springer Science and Business Media LLC. - 1471-2180. ; 28:8:18
-
Tidskriftsartikel (refereegranskat)abstract
- Aggregatibacter actinomycetemcomitans is an oral bacterium associated with aggressively progressing periodontitis. Extracellular release of bacterial outer membrane proteins has been suggested to mainly occur via outer membrane vesicles. This study investigated the presence and conservation of peptidoglycan-associated lipoprotein (AaPAL) among A. actinomycetemcomitans strains, the immunostimulatory effect of AaPAL, and whether live cells release this structural outer membrane lipoprotein in free-soluble form independent of vesicles. RESULTS: The pal locus and its gene product were confirmed in clinical A. actinomycetemcomitans strains by PCR-restriction fragment length polymorphism and immunoblotting. Culturing under different growth conditions revealed no apparent requirement for the AaPAL expression. Inactivation of pal in a wild-type strain (D7S) and in its spontaneous laboratory variant (D7SS) resulted in pleiotropic cellular effects. In a cell culture insert model (filter pore size 0.02 um), AaPAL was detected from filtrates when strains D7S and D7SS were incubated in serum or broth in the inserts. Electron microscopy showed that A. actinomycetemcomitans vesicles (0.05-0.2 um) were larger than the filter pores and that there were no vesicles in the filtrates. The filtrates were immunoblot negative for a cytoplasmic marker, cyclic AMP (cAMP) receptor protein. An ex vivo model indicated cytokine production from human whole blood stimulated by AaPAL. CONCLUSIONS: Free-soluble AaPAL can be extracellularly released in a process independent of vesicles.
|
|
| 22. |
- Lam, Y. Y., et al.
(författare)
-
Effects of dietary fat profile on gut permeability and microbiota and their relationships with metabolic changes in mice
- 2015
-
Ingår i: Obesity. - : Wiley. - 1930-7381. ; 23:7, s. 1429-1439
-
Tidskriftsartikel (refereegranskat)abstract
- ObjectiveTo distinguish the effects of dietary fat profile on gut parameters and their relationships with metabolic changes and to determine the capacity of n-3 fatty acids to modify gut variables in the context of diet-induced metabolic dysfunctions. MethodsMice received control or high-fat diets emphasizing saturated (HFD-sat), n-6 (HFD-n6), or n-3 (HFD-n3) fatty acids for 8 weeks. In another cohort, mice that were maintained on HFD-sat received n-3-rich fish oil or resolvin D1 supplementation. ResultsHFD-sat and HFD-n6 induced similar weight gain, but only HFD-sat increased index of insulin resistance (HOMA-IR), colonic permeability, and mesenteric fat inflammation. Hydrogen sulfide-producing bacteria were one of the major groups driving the diet-specific changes in gut microbiome, with the overall microbial profile being associated with changes in body weight, HOMA-IR, and gut permeability. In mice maintained on HFD-sat, fish oil and resolvin D1 restored barrier function and reduced inflammation in the colon but were unable to normalize HOMA-IR. ConclusionsDifferent dietary fat profiles led to distinct intestinal and metabolic outcomes that are independent of obesity. Interventions targeting inflammation successfully restored gut health but did not reverse systemic aspects of diet-induced metabolic dysfunction, implicating separation between gut dysfunctions and disease-initiating and/or -maintaining processes.
|
|
| 23. |
- Latva-Rasku, Aino, et al.
(författare)
-
The SGLT2 Inhibitor Dapagliflozin Reduces Liver Fat but Does Not Affect Tissue Insulin Sensitivity : A Randomized, Double-Blind, Placebo-Controlled Study With 8-Week Treatment in Type 2 Diabetes Patients.
- 2019
-
Ingår i: Diabetes Care. - : American Diabetes Association. - 0149-5992 .- 1935-5548. ; 42:5, s. 931-937
-
Tidskriftsartikel (refereegranskat)abstract
- OBJECTIVE: The aim of this study was to investigate tissue-specific effects of dapagliflozin on insulin sensitivity and liver and body fat in patients with type 2 diabetes.RESEARCH DESIGN AND METHODS: This randomized, double-blind, parallel group, placebo-controlled study recruited 32 patients with type 2 diabetes. Enrolled patients were to have HbA1c 6.5-10.5% (48-91 mmol/mol) and ≥3 months of stable treatment with metformin, dipeptidyl peptidase 4 inhibitor, or their combination. Patients were randomized 1:1 to receive 10 mg dapagliflozin or placebo daily for 8 weeks. Before and after the intervention, tissue insulin sensitivity was measured using [18F]-fluorodeoxyglucose and positron emission tomography during hyperinsulinemic-euglycemic clamp. Liver proton density fat fraction (PDFF) and adipose tissue volumes were assessed using MRI, and blood biomarkers were analyzed.RESULTS: After 8 weeks, glycemic control was improved by dapagliflozin (placebo-corrected change in HbA1c -0.39%, P < 0.01), but whole-body glucose uptake was not increased (P = 0.90). Tissue-specific insulin-stimulated glucose uptake did not change in skeletal muscle, liver, myocardium, or white and brown adipose tissue, and endogenous glucose production remained unaffected. However, there were significant placebo-corrected decreases in liver PDFF (-3.74%, P < 0.01), liver volume (-0.10 L, P < 0.05), visceral adipose tissue volume (-0.35 L, P < 0.01), interleukin-6 (-1.87 pg/mL, P < 0.05), and N-terminal prohormone of brain natriuretic peptide (-96 ng/L, P = 0.03).CONCLUSIONS: In this study, 8 weeks of treatment with dapagliflozin reduced liver PDFF and the volume of visceral adipose tissue in obese patients with type 2 diabetes. Although glycemic control was improved, no effect on tissue-level insulin sensitivity was observed.
|
|
| 24. |
|
|
| 25. |
|
|
| 26. |
|
|
| 27. |
- Lindkvist, Björn, et al.
(författare)
-
Importance of pancreatic exocrine dysfunction in patients with type 2 diabetes: A randomized crossover study
- 2018
-
Ingår i: Pancreatology. - : Elsevier BV. - 1424-3903. ; 18:5, s. 550-558
-
Tidskriftsartikel (refereegranskat)abstract
- Background: Levels of faecal elastase-1 (FE-1), a marker of exocrine pancreatic function, are lower in patients with type 2 diabetes than without diabetes. We aimed to investigate the association between FE-1 and nutritional status, gastrointestinal symptoms, and lipid absorption. Methods: This randomized, open-label, crossover study included 315 patients with type 2 diabetes aged 18-70 years treated with oral antidiabetics, with HbA(1c) 6.59.0% and BMI 1840 kg/m(2). Assessments included levels of FE-1 and blood biomarkers of nutrition, and Bristol Stool Scale and Gastrointestinal Symptom Rating Scale (GSRS) scores. Plasma exposure of docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) after oral administration of free omega-3 carboxylic acids or ethyl esters with breakfast was investigated in patients with low, intermediate, and normal FE-1 levels. Results: The prevalence of low and intermediate FE-1 levels was 5.2% and 4.9%, respectively. Bristol Stool Scale scores and mean values of GSRS Diarrhoea and Indigestion domain symptoms were similar across groups, but patients with low FE-1 were heavier and reported lower stool frequency. FE-1 levels correlated positively with plasma levels of amylase, lipase, 25-hydroxy vitamin D, and albumin. Mean EPA + DHA exposure was similarly higher after intake of free vs. esterified omega-3 fatty acids in all FE-1 groups. Conclusions: The prevalence of low FE-1 (< 100 mu g/g) as a measure of pancreatic exocrine insufficiency was infrequent in type 2 diabetes. Except for low plasma concentrations of EPA and 25-hydroxy vitamin D, type 2 diabetes patients with low FE-1 had no other signs of malabsorption or gastrointestinal disorders. Plasma levels of EPA and DHA after the intake of esterified versus free EPA and DHA did not correlate with FE-1 levels. (C) 2018 IAP and EPC. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license.
|
|
| 28. |
- Morshedzadeh, Iman, 1979-, et al.
(författare)
-
Managing virtual factory artifacts in the extended PLM context
- 2022
-
Ingår i: Journal of Industrial Information Integration. - : Elsevier. - 2467-964X .- 2452-414X. ; 28
-
Tidskriftsartikel (refereegranskat)abstract
- Virtual engineering increases the rate of and diversity of models being created; hence requires maintenance in a product lifecycle management (PLM) system. This also induces the need to understand their creation contexts, known as historical or provenance information, to reuse the models in other engineering projects. PLM systems are specifically designed to manage product- and production-related data. However, they are less capable of handling the knowledge about the contexts of the models without an appropriate extension. Therefore, this research proposes an extension to PLM systems by designing a new information model to contain virtual models, their related data and knowledge generated from them through various engineering activities so that they can be effectively used to manage historical information related to all these virtual factory artifacts. Such an information model is designed to support a new Virtual Engineering ontology for capturing and representing virtual models and engineering activities, tightly integrated with an extended provenance model based on the W7 model. In addition, this paper presents how an application prototype, called Manage-Links, has been implemented with these extended PLM concepts and then used in several virtual manufacturing activities in an automotive company.
|
|
| 29. |
- Morshedzadeh, Iman, 1979-, et al.
(författare)
-
Multi-level management of discrete event simulation models in a product lifecycle management framework
- 2018
-
Ingår i: Procedia Manufacturing. - : Elsevier. - 2351-9789. ; 25, s. 74-81
-
Tidskriftsartikel (refereegranskat)abstract
- Discrete event simulation (DES) models imitates the behavior of a production system. Models can be developed to reflect different levels of the production system, e.g supply chain level or manufacturing line level. Product Lifecycle Management (PLM) systems have been developed in order to manage product and manufacturing related data. DES models is one kind of product lifecycle’s data which can be managed by a PLM system. This paper presents a method and its implementation for management of interacting multi-level models utilizing a PLM system.
|
|
| 30. |
- Morshedzadeh, Iman, 1979-, et al.
(författare)
-
Product lifecycle management with provenance management and virtual models : an industrial use-case study
- 2018
-
Ingår i: Procedia CIRP. - : Elsevier. - 2212-8271. ; 72, s. 1190-1195
-
Tidskriftsartikel (refereegranskat)abstract
- Saving and managing virtual models’ provenance information (models’ history) can increase the level of reusability of those models. This paper describes a provenance management system (PMS) that has been developed based on an industrial case study.The product lifecycle management (PLM) system, as a main data management system, is responsible for receiving virtual models and their related data from Computer-Aided technologies (CAx) and providing this information for the PMS. In this paper, the management of discrete event simulation data with the PLM system will be demonstrated as the first link of provenance data management chain (CAx-PLM-PMS).
|
|
| 31. |
- Morshedzadeh, Iman, 1979-, et al.
(författare)
-
Real World Data Identification and Classification for Support of Virtual Confidence
- 2016
-
Konferensbidrag (refereegranskat)abstract
- Shortening of the product development process time is one of the main approaches for all enterprises to offer their products to the market. Virtual manufacturing tools can help companies to reduce their time to market, by reduction of the engineering lead time. Extensive use of virtual engineering models results in a need for verification of the model’s accuracy. This virtual engineering usability and assessment have been named virtual confidence. The two main factors of the achievement of this confidence are the accuracy of the virtual models and the virtual engineering results.For controlling of both above factors, a complete virtual model and related virtual model knowledge are needed. These knowledges can be tacit or explicit. For exploring explicit knowledge, a data and information collection from different disciplines in the organization is needed.In this paper, a data map with focus on the manufacturing engineering scope will be presented. This data map is generated from different data sources at a manufacturing plant, and gives an overview of different data that exist at different data sources, in the area of manufacturing. Combining real world data from different sources with virtual engineering model data supports, amongst others, establishment of virtual confidence.
|
|
| 32. |
- Oscarsson, A, et al.
(författare)
-
Reorganization of actin in neurons after propofol exposure
- 2001
-
Ingår i: Acta Anaesthesiologica Scandinavica. - 0001-5172. ; 45:10, s. 1215-1220
-
Tidskriftsartikel (refereegranskat)abstract
- BACKGROUND: It has previously been shown that propofol in clinically relevant concentrations induces a calcium-dependent conformational change in the cytoskeleton. The aim of this study was to further clarify the effect of propofol on the actin cytoskeleton and to determine if this conformational change is mediated by the interaction between the GABA(A)-receptor and propofol. METHODS: Primary cultured cortical neurons from newborn rats were treated with propofol 3 microg x ml(-1) in a time-response titration, with and without preincubation with the GABA(A)-receptor antagonist, bicuculline. Actin-protein content was detected by Western blot analysis and the cellular content of F-actin measured by a spectrophotometric technique. RESULTS: Propofol triggers a relatively slow statistically significant increase in the intracellular F-actin content, maximum after 20-min incubation (160%+/-16.3) (mean+/-SEM) P<0.05. The propofol-induced increase in F-actin was effectively blocked by bicuculline. The increase in intracellular actin content after exposure to propofol as well as the effect of bicuculline were verified by Western blot analysis. CONCLUSION: The present study shows that propofol triggers a time-dependent change of actin. Since this reorganization can be blocked effectively by a GABA(A)-receptor antagonist, this suggests that the GABA(A)-receptor is involved in the pathway leading to cytoskeletal reorganization after propofol treatment. The actin polymerization reached its maximum after 20 min. Therefore, we believe that the propofol-induced changes might be connected with slower cellular responses such as cell-to-cell interaction and/or channel regulation.
|
|
| 33. |
- Oscarsson, E., et al.
(författare)
-
Commercial infant cereals contain high concentrations of endotoxins and viable Bacillus spp.
- 2020
-
Ingår i: Food Control. - : Elsevier BV. - 0956-7135. ; 118
-
Tidskriftsartikel (refereegranskat)abstract
- Background: Endotoxin may cause inflammation and increased intestinal permeability in infants with immature immune systems and gut microbiota. This study analysed the presence of endotoxin together with other potentially hazardous agents in commercial infant cereals. Methods: Four porridges and six milk cereal drinks bought in Sweden were analysed for the presence of endotoxins using Endosafe MCS. The quantity of viable bacteria was determined using conventional culturing methods and the isolates were identified with Sanger sequencing. Enterotoxin producing genes were analysed through multiplex PCR and levels of mycotoxins were studied using uHPLC MS/MS. Results: The endotoxin concentration ranged from 1400 to 24200 EU/g powder. Viable bacteria were found in the products, indicating survivability of Bacillus spp in dry powders. No traces of mycotoxins were found. Conclusion: This study indicates that substantial concentrations of endotoxins are present in powdered infant cereal-based foods. Furthermore, the amount of living bacteria ingested with some of the products was high.
|
|
| 34. |
|
|
| 35. |
|
|
| 36. |
|
|
| 37. |
- Sydsjö, Gunilla, et al.
(författare)
-
Knowledge of reproduction in teenagers and young adults in Sweden
- 2006
-
Ingår i: European journal of contraception & reproductive health care. - : Informa UK Limited. - 1362-5187 .- 1473-0782. ; 11:2, s. 117-125
-
Tidskriftsartikel (refereegranskat)abstract
- Objective: To investigate the knowledge of reproductive physiology and anatomy among adolescents and young adults in Sweden, and to evaluate the education obtained on these issues. Methods: Two hundred and nine study specific questionnaires were distributed, of which 206 were answered by students in primary school, upper secondary school and at first year of university. A total knowledge score based on 21 out of the 35 questions in the questionnaire was calculated. As only 44 of the 206 respondents answered all the questions a revised score was also calculated, in which partial dropouts were interpreted as wrong answers. Results: The mean of the total knowledge score was 28.7 out of 54 among the 44 respondents answering all questions, and the revised knowledge score was 24.0. The level of knowledge tended to be higher in older age groups, among women, as well as among respondents who had visited a Youth clinic. The time of ovulation was known by 21.4% of men compared to 63.4% of women. Almost 50% of the students knew one mechanism whereby oral contraceptives act to protect against pregnancy. Of the respondents, 77.2% knew of Chlamydia trachomatis but the knowledge of other STIs, like condyloma, was poorer (16.5%). Reasons for infertility were relatively well known among the respondents. Of the respondents, 57.5% stated that they had not received enough information on reproductive issues. Conclusions: The students have not achieved sufficient knowledge concerning sexual and reproductive matters that they, according to the national curriculum, should have attained by the end of the 9th grade. An improvement of the quality of the education, adapted to the students' age and pre-existing knowledge, and a review of the contents of the education is therefore needed. © 2006 European Society of Contraception.
|
|
| 38. |
- Uhlig, E., et al.
(författare)
-
Use of bacterial strains antagonistic to Escherichia coli for biocontrol of spinach : A field trial
- 2021
-
Ingår i: Innovative Food Science and Emerging Technologies. - : Elsevier BV. - 1466-8564. ; 74
-
Tidskriftsartikel (refereegranskat)abstract
- To counteract global food safety hazards related to raw consumption of ready-to-eat leafy vegetables, a method to improve bacterial status using antagonistic bacteria was studied under field conditions. This is the first study to identify potential Escherichia coli antagonists from the native microbiota on leafy green vegetables and evaluate their effect in an industrial field production setting. Bacterial strains were isolated from different types of leafy green vegetables and selected upon their effect against E. coli in vitro, and out of 295 tested bacterial strains, 37 showed an antagonistic effect. Four of those antagonistic strains were coated in separate treatments onto spinach seeds and planted in the field. Both seeds and plants were analyzed by Illumina MiSeq next generation sequencing (NGS), and it was seen that the microbiota of the plants contained lower relative abundance of plant and human pathogenic genera. Higher β-diversity was observed for the samples treated with Bacillus coagulans LMG P-32205 and B. coagulans LMG P-32206 compared to control, indicating that those strains have induced substantial changes in the native microbiota of the leaves. A reduction of Escherichia-Shigella was seen for two of the isolates (Pseudomonas cedrina LMG P-32207 and Pseudomonas punonenis LMG P-32204) as the seeds developed into plants. Seeds inoculated with two of the strains (B. coagulans LMG P-32205 and B. coagulans LMG P-32206) had increased levels of Lactobacillaceae, and treatment with B. coagulans LMG P-32206 resulted in lower levels of Pantoea (from 31.4 to 12.2%). These results encourage the usage of bacterial antagonists as part of a global solution to reduce the risk of human pathogens on leafy green vegetables.
|
|
