| 1. |
- Cossarizza, A., et al.
(författare)
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Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition)
- 2019
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Ingår i: European Journal of Immunology. - : Wiley. - 0014-2980 .- 1521-4141. ; 49:10, s. 1457-1973
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Tidskriftsartikel (refereegranskat)abstract
- These guidelines are a consensus work of a considerable number of members of the immunology and flow cytometry community. They provide the theory and key practical aspects of flow cytometry enabling immunologists to avoid the common errors that often undermine immunological data. Notably, there are comprehensive sections of all major immune cell types with helpful Tables detailing phenotypes in murine and human cells. The latest flow cytometry techniques and applications are also described, featuring examples of the data that can be generated and, importantly, how the data can be analysed. Furthermore, there are sections detailing tips, tricks and pitfalls to avoid, all written and peer-reviewed by leading experts in the field, making this an essential research companion.
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| 2. |
- Polme, S., et al.
(författare)
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FungalTraits: a user-friendly traits database of fungi and fungus-like stramenopiles
- 2020
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Ingår i: Fungal Diversity. - : Springer Science and Business Media LLC. - 1560-2745 .- 1878-9129. ; 105:1, s. 1-16
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Tidskriftsartikel (refereegranskat)abstract
- The cryptic lifestyle of most fungi necessitates molecular identification of the guild in environmental studies. Over the past decades, rapid development and affordability of molecular tools have tremendously improved insights of the fungal diversity in all ecosystems and habitats. Yet, in spite of the progress of molecular methods, knowledge about functional properties of the fungal taxa is vague and interpretation of environmental studies in an ecologically meaningful manner remains challenging. In order to facilitate functional assignments and ecological interpretation of environmental studies we introduce a user friendly traits and character database FungalTraits operating at genus and species hypothesis levels. Combining the information from previous efforts such as FUNGuild and Fun(Fun) together with involvement of expert knowledge, we reannotated 10,210 and 151 fungal and Stramenopila genera, respectively. This resulted in a stand-alone spreadsheet dataset covering 17 lifestyle related traits of fungal and Stramenopila genera, designed for rapid functional assignments of environmental studies. In order to assign the trait states to fungal species hypotheses, the scientific community of experts manually categorised and assigned available trait information to 697,413 fungal ITS sequences. On the basis of those sequences we were able to summarise trait and host information into 92,623 fungal species hypotheses at 1% dissimilarity threshold.
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| 3. |
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| 4. |
- Erikson, A, et al.
(författare)
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The near-Earth objects follow-up program - III. 3 lightcurves for 12 objects from 1992 and 1995
- 2000
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Ingår i: ICARUS. - : ACADEMIC PRESS INC. - 0019-1035. ; 147:2, s. 487-497
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Tidskriftsartikel (refereegranskat)abstract
- In this paper we present lightcurves and physical parameters for nine near-Earth objects (NEOs) (433 Eros, 1917 Cuyo, 2368 Beltrovata, 3753 Cruithne, 4769 Castalia, 4953 1990 MU, 6491 1991 OA, 1995 EK1, and 1995 FJ) and three Mars crossers (5738 Billpicke
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| 5. |
- Piironen, J, et al.
(författare)
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Physical studies of asteroids - XXXII. Rotation periods and UBVRI-colours for selected asteroids
- 1998
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Ingår i: ASTRONOMY & ASTROPHYSICS SUPPLEMENT SERIES. - : E D P SCIENCES. - 0365-0138. ; 128:3, s. 525-540
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Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)abstract
- We present lightcurves of selected asteroids. Most of the asteroids were included to obtain refined spin periods. Enhanced periods were determined for 11 Parthenope, 306 Unitas and 372 Palma. We confirmed the spin periods of 8 Flora, 13 Egeria, 71 Niobe,
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| 6. |
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| 7. |
- Huber, Johannes A. J., 1989-, et al.
(författare)
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A method for generating finite element models of wood boards from X-ray computed tomography scans
- 2022
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Ingår i: Computers & structures. - : Elsevier. - 0045-7949 .- 1879-2243. ; 260
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Tidskriftsartikel (refereegranskat)abstract
- A method is presented for reconstructing the geometry, the pith, the knots and the local fibre orientations in timber boards, based on X-ray computed tomography scans. The local fibre deviations around knots were found by a new algorithm, based on image analysis. The experimental data comprised tomography scans, eigenfrequency measurements and four-point bending tests of 20 Norway spruce boards. 3D and 1D finite element models of the pure bending zone of the bending tests were created, accounting for the exact board geometry and the reconstructed fibre deviations. A purely density based, a purely eigenfrequency based, and a mixed constitutive law were compared. Model estimations showed a high coefficient of determination (R2) for global modulus of elasticity (MoE) (R2⩽0.93), local MoE (R2⩽0.87), bending strength (R2⩽0.83), and the location of initial failure. Constitutive laws accounting for eigenfrequency showed the most accurate results. In the future, adapting the method for logs could enable analyses of boards before sawing.
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| 8. |
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| 9. |
- Kõljalg, Urmas, et al.
(författare)
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Towards a unified paradigm for sequence-based identification of fungi.
- 2013
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Ingår i: Molecular ecology. - : Wiley. - 1365-294X .- 0962-1083. ; 22:21, s. 5271-7
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Tidskriftsartikel (refereegranskat)abstract
- The nuclear ribosomal internal transcribed spacer (ITS) region is the formal fungal barcode and in most cases the marker of choice for the exploration of fungal diversity in environmental samples. Two problems are particularly acute in the pursuit of satisfactory taxonomic assignment of newly generated ITS sequences: (i) the lack of an inclusive, reliable public reference data set and (ii) the lack of means to refer to fungal species, for which no Latin name is available in a standardized stable way. Here, we report on progress in these regards through further development of the UNITE database (http://unite.ut.ee) for molecular identification of fungi. All fungal species represented by at least two ITS sequences in the international nucleotide sequence databases are now given a unique, stable name of the accession number type (e.g. Hymenoscyphus pseudoalbidus|GU586904|SH133781.05FU), and their taxonomic and ecological annotations were corrected as far as possible through a distributed, third-party annotation effort. We introduce the term 'species hypothesis' (SH) for the taxa discovered in clustering on different similarity thresholds (97-99%). An automatically or manually designated sequence is chosen to represent each such SH. These reference sequences are released (http://unite.ut.ee/repository.php) for use by the scientific community in, for example, local sequence similarity searches and in the QIIME pipeline. The system and the data will be updated automatically as the number of public fungal ITS sequences grows. We invite everybody in the position to improve the annotation or metadata associated with their particular fungal lineages of expertise to do so through the new Web-based sequence management system in UNITE.
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| 10. |
- LAGERKVIST, CI, et al.
(författare)
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PHYSICAL STUDIES OF ASTEROIDS .29. PHOTOMETRY AND ANALYSIS OF 27 ASTEROIDS
- 1995
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Ingår i: ASTRONOMY & ASTROPHYSICS SUPPLEMENT SERIES. - : EDITIONS PHYSIQUE. - 0365-0138. ; 113:1, s. 115-129
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Tidskriftsartikel (refereegranskat)abstract
- In this paper photometry and analysis of 27 asteroids are presented. For the asteroids 21 Lutetia, 23 Thalia, 250 Bettina and 135 Hertha spin vector determinations were made. For asteroid 99 Dike we derived a rotation period of 10.35 hours and for 1280 Ba
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| 11. |
- Oja, C., et al.
(författare)
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Awareness of parental illness: a grounded theory of upholding family equilibrium in parents on long-term sick-leave in primary health care
- 2021
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Ingår i: Scandinavian Journal of Primary Health Care. - : Informa UK Limited. - 0281-3432 .- 1502-7724. ; 39:3, s. 268-278
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Tidskriftsartikel (refereegranskat)abstract
- Objective To understand the main concern of chronically ill parents and how they resolve this concern in relation to their children. Design Grounded theory. Setting Three primary health care clinics in Sweden. Subjects Thirty-two interviewed parents and their children. Main outcome measures Processes and typologies of upholding family relationships. Results A concern of chronically ill parents is sustaining family equilibrium, achieved through a process of upholding family relationships. How a parent upholds depends upon his/her comprehension of the illness and of their child's need for parenting. In response to the parent's upholding behaviours, children mirror the effect of the illness to the parent, the child's specific behaviour depending on his/her level of comprehension regarding the parent's illness. Their combined behaviours create an awareness context that may be closed, concealed, suspicious, conflicted, mutual pretence or open. When the parent drives and facilitates the evolution of comprehension, the context quickly evolves from closed to open. When the parent hinders the process by masking and resisting the child responds by probing and proving and they become locked into a suspicious or conflicted awareness context with high relational tension. To create family equilibrium the parent needs to reveal and facilitate the awareness process. Conclusion Parents on long-term sick leave in primary health care can need assistance to facilitate the awareness context of themselves and their child. Implications: Clinicians can identify the current awareness context of their patient and help their patient towards increased understanding of their illness; their child's needs and the parental capacities needed to reveal the illness and its impacts.
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| 12. |
- Oja, C., et al.
(författare)
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Informing children of their parent's illness: A systematic review of intervention programs with child outcomes in all health care settings globally from inception to 2019
- 2020
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Ingår i: PLoS ONE. - : Public Library of Science (PLoS). - 1932-6203. ; 15:5
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Tidskriftsartikel (refereegranskat)abstract
- Introduction Children are impacted when parents are ill. This systematic review gives an overview of the current state of research and extracts what children and parents found helpful in the interventions aimed at informing children of their parent's illness. Methods This review was registered with PROSPERO and conducted in accordance with PRISMA guidelines. Five health and social science databases were searched from inception to November 2019 to identify original, peer-reviewed articles in English describing effective interventions. The authors selected and reviewed the studies independently, and any inconsistencies were resolved by discussion in face-to-face meetings and emails. A descriptive synthesis of evidence-based concepts from quantitative and qualitative studies was conducted. Results A total of 13 892 titles and 144 full-text articles were reviewed with 32 selected for final inclusion, 21 quantitative, 11 qualitative and no mixed-method studies published from 1993 to November 2019. Most of the research was conducted in mental health, including substance abuse (n = 22), but also in cancer care (n = 6) and HIV care (n = 4). Most studies using quantitative method showed a small to moderately positive statistically significant intervention effect on the child's level of internalized symptoms. Content analysis of the results of studies employing qualitative methodology resulted in four concepts important to both children and parents in interventions (increased knowledge, more open communication, new coping strategies and changed feelings) and three additional concepts important to parents (observed changes in their children's behavior, the parent's increased understanding of their own child and the relief of respite). Conclusions In the literature there is evidence of mild to moderate positive effects on the child's level of internalized symptoms as well as concepts important to children and parent's worth noting when trying to bridge the still existing knowledge gaps. In further efforts the challenges of implementation as well as adaptation to differing clinical and personal situations appear key to address.
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| 13. |
- Oja, C., et al.
(författare)
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Making life work for both themselves and their parents alone- how children aged 11-16 view themselves in relation to their chronically ill parents in a primary health care setting
- 2019
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Ingår i: International Journal of Adolescence and Youth. - : Informa UK Limited. - 0267-3843 .- 2164-4527. ; 24:4, s. 408-420
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Tidskriftsartikel (refereegranskat)abstract
- This study explores the main concern and life strategies of children whose parents are chronically ill in a Primary Health Care (PHC) center in Stockholm, Sweden. Data was collected through semi-structured individual interviews, and qualitative inductive constant comparative analysis resulted in a conceptual model. The two main strategies the children used were to understand the situation and to adopt a parental role. Children saw themselves as main contributors into the functioning of their families. Results show that these children also viewed their situation as difficult. It is an important mental health task for professionals in PHC to reach out and inform both ill parents and their children that children are very interested and involved in their parents' health. These children need respect for both their capacity and their vulnerability as they struggle to make life stable and functional for themselves and their parents. Further research and development of appropriate interventions are needed in this ethically challenging area.
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| 14. |
- Oja, L., et al.
(författare)
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Behavioral and electrophysiological indicators of auditory distractibility in children with ADHD and comorbid ODD
- 2016
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Ingår i: Brain Research. - : Elsevier BV. - 0006-8993 .- 1872-6240. ; 1632, s. 42-50
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Tidskriftsartikel (refereegranskat)abstract
- Involuntary switching of attention to distracting sounds was studied by measuring effects of these events on auditory discrimination performance and event-related brain potentials (ERPs) in 6–11-year-old boys with Attention Deficit – Hyperactivity Disorder (ADHD) and comorbid Oppositional Defiant Disorder (ODD) and in age-matched controls. The children were instructed to differentiate between two animal calls by pressing one response button, for example, to a dog bark and another button to a cat mew. These task-relevant sounds were presented from one of two loudspeakers in front of the child, and there were occasional task-irrelevant changes in the sound location, that is, the loudspeaker. In addition, novel sounds (e.g., a sound of hammer, rain, or car horn) unrelated to the task were presented from a loudspeaker behind the child. The percentage of correct responses was lower for target sounds preceded by a novel sound than for targets not preceded by such sound in the ADHD group, but not in the control group. In both groups, a biphasic positive P3a response was observed in ERPs to the novel sounds. The later part of the P3a appeared to continue longer over the frontal scalp areas in the ADHD group than in the controls presumably because a reorienting negativity (RON) ERP response following the P3a was smaller in the ADHD group than in the control group. This suggests that the children with ADHD had problems in reorienting their attention to the current task after a distracting novel sound leading to deterioration of performance in this task. The present study also indicates that children with ADHD and comorbid ODD show same kind of distractibility as found in previous studies for children with ADHD without systematic comorbid ODD.
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| 15. |
- Verbestel, V., et al.
(författare)
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Effectiveness of the IDEFICS intervention on objectively measured physical activity and sedentary time in European children
- 2015
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Ingår i: Obesity Reviews. - : Wiley. - 1467-7881 .- 1467-789X. ; 16:Suppl. 2, s. 57-67
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Tidskriftsartikel (refereegranskat)abstract
- BackgroundThis paper reports on the effectiveness of the prevention of dietary- and lifestyle-induced health effects in children and infants (IDEFICS) intervention on objectively measured physical activity (PA) and sedentary time (ST) in 2- to 9.9-year-old European boys and girls. MethodsThe intervention was evaluated after 2years through a non-randomized cluster-controlled trial in eight European countries (one control and one intervention community per country). All children in the intervention group received a culturally adapted childhood obesity prevention programme through the community, schools/kindergartens and family. A random sub-sample of children participating in the IDEFICS study wore an accelerometer at baseline and follow-up for at least 3days (n=9,184). Of this sample, 81% provided valid accelerometer data at baseline (n=7,413; 51% boys; 6.211.76years; boys: 617170cpmday(-1); girls 556 +/- 156cpmday(-1)) and 3,010 children provided valid accelerometer data at baseline and during the follow-up survey 2years later. ResultsIn boys and girls, no significant differences in PA and ST were found between intervention and control groups over 2years. Strong temporal effects were found in the total sample of boys and girls: the percentage of time spent in light PA per day decreased by 4 percentage points in both boys and girls between baseline and follow-up (both: p<0.001), while time spent in ST per day increased by 4 percentage points in both sexes over time (both: p<0.001). Percentage of time spent in moderate-to-vigorous PA per day remained stable over time in boys and girls. ConclusionDespite the socio-ecological approach and implementation of a culturally adapted intervention in each country, no effects of the IDEFICS intervention were found on children's objectively measured PA and ST. Behavioural interventions for children may need to enhance specificity and intensity at the family level using other behaviour change techniques and more direct strategies to reach parents. (c) 2015 World Obesity
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| 16. |
- Weidenhiller, A., et al.
(författare)
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Improved strength grading based on log and board measurements - Review and outlook : The research project READistrength
- 2021
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Ingår i: World Conference on Timber Engineering 2021, WCTE 2021. - : World Conference on Timber Engineering (WCTE).
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Konferensbidrag (refereegranskat)abstract
- Timber utilisation and wood construction are fundamental components of Europe's strategy on progressing towards a sustainable bio-based economy. Both components require optimised strength grading procedures in Europe's sawmills. To further improve the current procedures, quality assessment and segregation of the raw material need to start already at the roundwood stage. Furthermore, the quality information should be made accessible throughout the production process to leverage the full potential for optimisation. This paper discusses the current state of research on combined log and board strength grading with a focus on the situation in Europe. It highlights limitations due to technology and current standardisation and identifies knowledge gaps and research opportunities. The European research project READiStrength (resource-efficient and data-driven integrated log and board strength grading) has been initiated to address these issues.
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| 17. |
- Wiebe, M. G., et al.
(författare)
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A novel aldose-aldose oxidoreductase for co-production of D-xylonate and xylitol from D-xylose with Saccharomyces cerevisiae
- 2015
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Ingår i: Applied Microbiology and Biotechnology. - : Springer Science and Business Media LLC. - 1432-0614 .- 0175-7598. ; 99:22, s. 9439-47
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Tidskriftsartikel (refereegranskat)abstract
- An open reading frame CC1225 from the Caulobacter crescentus CB15 genome sequence belongs to the Gfo/Idh/MocA protein family and has 47 % amino acid sequence identity with the glucose-fructose oxidoreductase from Zymomonas mobilis (Zm GFOR). We expressed the ORF CC1225 in the yeast Saccharomyces cerevisiae and used a yeast strain expressing the gene coding for Zm GFOR as a reference. Cell extracts of strains overexpressing CC1225 (renamed as Cc aaor) showed some Zm GFOR type of activity, producing D-gluconate and D-sorbitol when a mixture of D-glucose and D-fructose was used as substrate. However, the activity in Cc aaor expressing strain was >100-fold lower compared to strains expressing Zm gfor. Interestingly, C. crescentus AAOR was clearly more efficient than the Zm GFOR in converting in vitro a single sugar substrate D-xylose (10 mM) to xylitol without an added cofactor, whereas this type of activity was very low with Zm GFOR. Furthermore, when cultured in the presence of D-xylose, the S. cerevisiae strain expressing Cc aaor produced nearly equal concentrations of D-xylonate and xylitol (12.5 g D-xylonate l(-1) and 11.5 g D-xylitol l(-1) from 26 g D-xylose l(-1)), whereas the control strain and strain expressing Zm gfor produced only D-xylitol (5 g l(-1)). Deletion of the gene encoding the major aldose reductase, Gre3p, did not affect xylitol production in the strain expressing Cc aaor, but decreased xylitol production in the strain expressing Zm gfor. In addition, expression of Cc aaor together with the D-xylonolactone lactonase encoding the gene xylC from C. crescentus slightly increased the final concentration and initial volumetric production rate of both D-xylonate and D-xylitol. These results suggest that C. crescentus AAOR is a novel type of oxidoreductase able to convert the single aldose substrate D-xylose to both its oxidized and reduced product.
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