| 1. |
- Cossarizza, A., et al.
(författare)
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Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition)
- 2019
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Ingår i: European Journal of Immunology. - : Wiley. - 0014-2980 .- 1521-4141. ; 49:10, s. 1457-1973
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Tidskriftsartikel (refereegranskat)abstract
- These guidelines are a consensus work of a considerable number of members of the immunology and flow cytometry community. They provide the theory and key practical aspects of flow cytometry enabling immunologists to avoid the common errors that often undermine immunological data. Notably, there are comprehensive sections of all major immune cell types with helpful Tables detailing phenotypes in murine and human cells. The latest flow cytometry techniques and applications are also described, featuring examples of the data that can be generated and, importantly, how the data can be analysed. Furthermore, there are sections detailing tips, tricks and pitfalls to avoid, all written and peer-reviewed by leading experts in the field, making this an essential research companion.
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| 2. |
- Polme, S., et al.
(författare)
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FungalTraits: a user-friendly traits database of fungi and fungus-like stramenopiles
- 2020
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Ingår i: Fungal Diversity. - : Springer Science and Business Media LLC. - 1560-2745 .- 1878-9129. ; 105:1, s. 1-16
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Tidskriftsartikel (refereegranskat)abstract
- The cryptic lifestyle of most fungi necessitates molecular identification of the guild in environmental studies. Over the past decades, rapid development and affordability of molecular tools have tremendously improved insights of the fungal diversity in all ecosystems and habitats. Yet, in spite of the progress of molecular methods, knowledge about functional properties of the fungal taxa is vague and interpretation of environmental studies in an ecologically meaningful manner remains challenging. In order to facilitate functional assignments and ecological interpretation of environmental studies we introduce a user friendly traits and character database FungalTraits operating at genus and species hypothesis levels. Combining the information from previous efforts such as FUNGuild and Fun(Fun) together with involvement of expert knowledge, we reannotated 10,210 and 151 fungal and Stramenopila genera, respectively. This resulted in a stand-alone spreadsheet dataset covering 17 lifestyle related traits of fungal and Stramenopila genera, designed for rapid functional assignments of environmental studies. In order to assign the trait states to fungal species hypotheses, the scientific community of experts manually categorised and assigned available trait information to 697,413 fungal ITS sequences. On the basis of those sequences we were able to summarise trait and host information into 92,623 fungal species hypotheses at 1% dissimilarity threshold.
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| 3. |
- Kaivo-Oja, Noora, et al.
(författare)
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Growth differentiation factor-9 induces Smad2 activation and inhibin B production in cultured human granulosa-luteal cells
- 2003
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Ingår i: Journal of Clinical Endocrinology and Metabolism. - : The Endocrine Society. - 0021-972X .- 1945-7197. ; 88:2, s. 755-762
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Tidskriftsartikel (refereegranskat)abstract
- The TGF beta family member growth differentiation factor-9 (GDF-9) is an oocyte-derived factor that is essential for mammalian ovarian folliculogenesis. GDF-9 mRNAs have been shown to be expressed in the human ovarian follicle from the primary follicle stage onward, and recombinant GDF-9 has been shown to promote human ovarian follicle growth in vitro. In this study with primary cultures of human granulosa-luteal (hGL) cells, we investigated whether recombinant GDF-9 activates components of the Smad signaling pathways known to be differentially activated by TGF beta and the bone morphogenetic proteins (BMPs). As with TGF beta, GDF-9 treatment caused the phosphorylation of endogenous 53-kDa proteins detected in Western blots with antiphospho-Smad2 antibodies (alpha PS2). However, unlike BMP-2, GDF-9 did not activate the phosphorylation of antiphospho-Smad1 antibody (alphaPS1)-immunoreactive proteins in hGL cells. Infection of hGL cells with an adenovirus expressing Smad2 (Ad-Smad2) confirmed that GDF-9 activates specifically phosphorylation of the Smad2 protein. Infection of hGL cells with Ad-Smad7, which expresses the inhibitory Smad7 protein, suppressed the levels of both GDF-9-induced endogenous and adenoviral alpha PS2-reactive proteins. Furthermore, GDF-9 increased the steady state levels of inhibin beta(B)-subunit mRNAs in hGL cells and strongly stimulated the secretion of dimeric inhibin B. Again, Ad-Smad7 blocked GDF-9-stimulated inhibin B production in a concentration-dependent manner. We identify here for the first time distinct molecular components of the GDF-9 signaling pathway in the human ovary. Our data suggest that GDF-9 mediates its effect through the pathway commonly activated by TGF beta and activin, but not that activated by many BMPs. The results are also consistent with the suggestion that in addition to endocrine control of inhibin production by gonadotropins, a local paracrine control of inhibin production is likely to occur via oocyte-derived factors in the human ovary.
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| 4. |
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| 5. |
- Kõljalg, Urmas, et al.
(författare)
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Towards a unified paradigm for sequence-based identification of fungi.
- 2013
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Ingår i: Molecular ecology. - : Wiley. - 1365-294X .- 0962-1083. ; 22:21, s. 5271-7
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Tidskriftsartikel (refereegranskat)abstract
- The nuclear ribosomal internal transcribed spacer (ITS) region is the formal fungal barcode and in most cases the marker of choice for the exploration of fungal diversity in environmental samples. Two problems are particularly acute in the pursuit of satisfactory taxonomic assignment of newly generated ITS sequences: (i) the lack of an inclusive, reliable public reference data set and (ii) the lack of means to refer to fungal species, for which no Latin name is available in a standardized stable way. Here, we report on progress in these regards through further development of the UNITE database (http://unite.ut.ee) for molecular identification of fungi. All fungal species represented by at least two ITS sequences in the international nucleotide sequence databases are now given a unique, stable name of the accession number type (e.g. Hymenoscyphus pseudoalbidus|GU586904|SH133781.05FU), and their taxonomic and ecological annotations were corrected as far as possible through a distributed, third-party annotation effort. We introduce the term 'species hypothesis' (SH) for the taxa discovered in clustering on different similarity thresholds (97-99%). An automatically or manually designated sequence is chosen to represent each such SH. These reference sequences are released (http://unite.ut.ee/repository.php) for use by the scientific community in, for example, local sequence similarity searches and in the QIIME pipeline. The system and the data will be updated automatically as the number of public fungal ITS sequences grows. We invite everybody in the position to improve the annotation or metadata associated with their particular fungal lineages of expertise to do so through the new Web-based sequence management system in UNITE.
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| 6. |
- Sattler, MC, et al.
(författare)
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Physical activity self-reports: past or future?
- 2021
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Ingår i: British journal of sports medicine. - : BMJ. - 1473-0480 .- 0306-3674. ; 55:16, s. 889-
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Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)
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| 7. |
- Chillon, P, et al.
(författare)
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Active commuting to school in children and adolescents: an opportunity to increase physical activity and fitness
- 2010
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Ingår i: Scandinavian journal of public health. - : SAGE Publications. - 1651-1905 .- 1403-4948. ; 38:8, s. 873-879
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Tidskriftsartikel (refereegranskat)abstract
- Aims: The purpose was to describe the patterns of commuting to school in young people and to examine its associations with physical activity (PA) and cardiorespiratory fitness. Methods: The sample comprised 2271 Estonian and Swedish children and adolescents (1218 females) aged 9—10 years and 15—16 years. Data were collected in 1998/99. Mode of commuting to and from school was assessed by questionnaire. Time spent (min/day) in PA and average PA (counts/min) was measured by accelerometry. Cardiorespiratory fitness was assessed by means of a maximal cycle ergometer test. Results: Sixty-one percent of the participants reported active commuting to school (ACS). Estonian youth showed lower levels of ACS than Swedish (odds ratio, 0.64; 95% confidence interval, 0.53—0.76) and girls reported lower levels than boys (0.74; 0.62—0.88). ACS boys showed higher PA levels than non-ACS boys for moderate, vigorous, MVPA, and average PA levels (all p ≤ 0.01). Participants who cycled to school had higher cardiorespiratory fitness than walkers or passive travellers (p < 0.001). Conclusions: Nearly two-thirds of the participants actively commuted to school. ACS may provide an opportunity to increase levels of daily PA, especially in boys, and cardiorespiratory fitness, especially if cycling. Public health strategies should develop and test ACS patterns to get more evidence and promote bike-friendly environments.
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| 8. |
- Daugbjerg, SB, et al.
(författare)
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Promotion of physical activity in the European region: content analysis of 27 national policy documents
- 2009
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Ingår i: Journal of physical activity & health. - : Human Kinetics. - 1543-3080 .- 1543-5474. ; 6:6, s. 805-817
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Tidskriftsartikel (refereegranskat)abstract
- Over the past years there has been increasing interest in physical activity promotion and the development of appropriate policy. So far, there has been no comprehensive overview of the activities taking place in Europe in this area of public health policy.Methods:Using different search methods, 49 national policy documents on physical activity promotion were identified. An analysis grid covering key features was developed for the analysis of the 27 documents published in English.Results:Analysis showed that many general recommendations for policy developments are being followed, for example: general goals were formulated, an implementation plan was included, a timeframe and a responsible body for the implementation was often specified. However, limited evidence for intersectoral collaboration was found. Quantified goals for physical activity were the exception. Population groups most in need such as people with low levels of physical activity were rarely specifically targeted. Most policies emphasized the importance of an evaluation. However, only about half of them indicated a related intention or requirement.Conclusion:In recent years there has been a noticeable development of national policy documents on physical activity promotion. Following principles for policy development more closely could increase the effectiveness of their preparation and implementation further.
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| 9. |
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| 10. |
- De Miguel-Etayo, P, et al.
(författare)
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Physical fitness reference standards in European children: the IDEFICS study.
- 2014
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Ingår i: International Journal of Obesity. - : Springer Science and Business Media LLC. - 0307-0565 .- 1476-5497. ; 38:2
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Tidskriftsartikel (refereegranskat)abstract
- BACKGROUND/OBJECTIVES: A low fitness status during childhood and adolescence is associated with important health-related outcomes, such as increased future risk for obesity and cardiovascular diseases, impaired skeletal health, reduced quality of life and poor mental health. Fitness reference values for adolescents from different countries have been published, but there is a scarcity of reference values for pre-pubertal children in Europe, using harmonised measures of fitness in the literature. The IDEFICS study offers a good opportunity to establish normative values of a large set of fitness components from eight European countries using common and well-standardised methods in a large sample of children. Therefore, the aim of this study is to report sex- and age-specific fitness reference standards in European children. SUBJECTS/METHODS: Children (10,302) aged 6-10.9 years (50.7% girls) were examined. The test battery included: the flamingo balance test, back-saver sit-and-reach test (flexibility), handgrip strength test, standing long jump test (lower-limb explosive strength) and 40-m sprint test (speed). Moreover, cardiorespiratory fitness was assessed by a 20-m shuttle run test. Percentile curves for the 1st, 3rd, 10th, 25th, 50th, 75th, 90th, 97th and 99th percentiles were calculated using the General Additive Model for Location Scale and Shape (GAMLSS). RESULTS: Our results show that boys performed better than girls in speed, lower- and upper-limb strength and cardiorespiratory fitness, and girls performed better in balance and flexibility. Older children performed better than younger children, except for cardiorespiratory fitness in boys and flexibility in girls. CONCLUSIONS: Our results provide for the first time sex- and age-specific physical fitness reference standards in European children aged 6-10.9 years.
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| 11. |
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| 12. |
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| 13. |
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| 14. |
- Ekblom, Örjan
(författare)
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Physical fitness and overweight in Swedish youths
- 2005
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Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- The aim of the present thesis was to describe the level of physical fitness and prevalence of overweight and obesity in Swedish youths, and their trends over 14 years. Additionally, the relation between fitness and overweight on the one hand and physical activity on the other was examined. In 2001, a total of 2118 children, aged 10, 13 and 16 years, were invited from 48 randomly selected schools in Sweden. Out of these, 1732 or 81.8 % participated in the testing. For trend analyses, data collected in 1987 from a sample of 516 children were used. When subjects in the 2001 sample were analysed according to proposed cut-off values, 15.7% (95% CI: 14.1% to 17.6%) of all subjects were found to be overweight and 4.3% (95% CI: 3.4% to 5.4%) were obese. The total prevalence accordingly being 20.1% (95% CI: 18.3% to 22.0%). This sample proved to be heterogeneous concerning overweight status. When obesity and overweight were combined the highest prevalence (26.6%, 95% CI: 21.2 to 32.7) was found in 10-year-old girls and the lowest (17.5%, 95% CI: 13.6 to 22.2) in 16-year-old girls. The prevalence in 2001 was nearly 2.5 times higher, compared to the 1987-sample. Girls attending schools in smaller towns or on the countryside had a nearly two-fold prevalence of overweight and obesity, compared to girls living in the major cities. The difference was less pronounced in boys. The prevalence of overweight and obesity was related to level of physical activity in 10 and 13 years old children but not in 16 years old. Results from the performance tests in the 1987 and 2001 samples were compared. Concerning cardio-respiratory performance, girls aged 16 years showed only small differences, whereas boys in the 2001 sample performed worse compared to boys in the 1987-sample (46 ml x kg-1 x min-1 vs. 41 ml x kg-1 x min-1 for 1987 and 2001, respectively, and median difference 9.2 %, 95% CI: -16.7 to -2.2). Concerning neuro-muscular performance, the comparisons of results between the 1987 and 2001 samples showed varying results. Generally, performance on the upper body and trunk muscles strength and endurance tests was lower in 2001 compared to 1987. Neuromuscular performance in the lower body differed less. For both boys and girls, differences in neuro-muscular performance between subjects in different activity strata increased with age. Results showed only small differences in neuro-muscular performance between active and less active strata at age 10, but larger at age 13 and 16 years in both boys and girls. And at age 13 and 16 years, neuro-muscular performance differed widely between active and less active girls. At age 10, only small differences were found in cardio-respiratory performance between active and less active subjects (both girls and boys). At age 13 and 16 years both girls and boys showed large differences in cardio-respiratory performance between activity levels, with better performance in more active children.
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| 15. |
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| 16. |
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| 17. |
- Hagstromer, M, et al.
(författare)
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Comparison of a subjective and an objective measure of physical activity in a population sample
- 2010
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Ingår i: Journal of physical activity & health. - : Human Kinetics. - 1543-3080 .- 1543-5474. ; 7:4, s. 541-550
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Tidskriftsartikel (refereegranskat)abstract
- The aim of this study was to compare physical activity components in the long, self-administrated version of IPAQ with an accelerometer in a population sample.Methods:In total 980 subjects (18-65 years) wore an accelerometer (Actigraph) for 7 consecutive days and thereafter filled in the IPAQ. Measures of total physical activity, time spent in moderate and in vigorous activity as well as time spent sitting as assessed by the IPAQ and the Actigraph were compared.Results:The results showed significant low to moderate correlations (Rs = 0.07−0.36) between the 2 instruments and significantly (P < .001) higher values for sitting and vigorous intensity physical activity from the IPAQ compared with the Actigraph. The higher the values reported by the IPAQ the bigger differences were seen between the instruments. Comparison between the tertiles of total physical activity by the 2 instruments showed significant overall association with consistent agreement in the low and the high tertiles.Conclusion:The long form of IPAQ is a valid measure of physical activity in population research. However, the IPAQ likely overestimates actual physical activity as shown by its limited ability to classify adults into low and high categories of physical activity based on accelerometer data.
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| 18. |
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| 19. |
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| 20. |
- LAGERKVIST, CI, et al.
(författare)
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PHYSICAL STUDIES OF ASTEROIDS .29. PHOTOMETRY AND ANALYSIS OF 27 ASTEROIDS
- 1995
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Ingår i: ASTRONOMY & ASTROPHYSICS SUPPLEMENT SERIES. - : EDITIONS PHYSIQUE. - 0365-0138. ; 113:1, s. 115-129
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Tidskriftsartikel (refereegranskat)abstract
- In this paper photometry and analysis of 27 asteroids are presented. For the asteroids 21 Lutetia, 23 Thalia, 250 Bettina and 135 Hertha spin vector determinations were made. For asteroid 99 Dike we derived a rotation period of 10.35 hours and for 1280 Ba
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| 21. |
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| 22. |
- Piironen, J, et al.
(författare)
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Physical studies of asteroids - XXXII. Rotation periods and UBVRI-colours for selected asteroids
- 1998
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Ingår i: ASTRONOMY & ASTROPHYSICS SUPPLEMENT SERIES. - : E D P SCIENCES. - 0365-0138. ; 128:3, s. 525-540
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Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)abstract
- We present lightcurves of selected asteroids. Most of the asteroids were included to obtain refined spin periods. Enhanced periods were determined for 11 Parthenope, 306 Unitas and 372 Palma. We confirmed the spin periods of 8 Flora, 13 Egeria, 71 Niobe,
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| 23. |
- Sepulveda, P., et al.
(författare)
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Modelling spiral grain in saw logs based on data form a simlated X-ray LogScanner
- 2004
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Ingår i: Fourth workshop "Connection between forest resources and wood quality: modelling approaches and simulation software" : Harrison Hot Springs, British Columbia, Canada, September 8 - 15, 2002 ; proceedings. - : International Union of Forestry Research Organisations.
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Konferensbidrag (övrigt vetenskapligt/konstnärligt)
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| 24. |
- Sugiyama, N, et al.
(författare)
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FGF receptor-4 (FGFR4) polymorphism acts as an activity switch of a membrane type 1 matrix metalloproteinase-FGFR4 complex
- 2010
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Ingår i: Proceedings of the National Academy of Sciences of the United States of America. - : Proceedings of the National Academy of Sciences. - 1091-6490. ; 107:36, s. 15786-15791
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Tidskriftsartikel (refereegranskat)abstract
- Tumor cells use membrane type 1 matrix metalloproteinase (MT1-MMP) for invasion and metastasis. However, the signaling mechanisms that underlie MT1-MMP regulation in cancer have remained unclear. Using a systematic gain-of-function kinome screen for MT1-MMP activity, we have here identified kinases that significantly enhance MT1-MMP activity in tumor cells. In particular, we discovered an MT1-MMP/FGF receptor-4 (FGFR4) membrane complex that either stimulates or suppresses MT1-MMP and FGFR4 activities, depending on a tumor progression-associated polymorphism in FGFR4. The FGFR4-R388 allele, linked to poor cancer prognosis, increased collagen invasion by decreasing lysosomal MT1-MMP degradation. FGFR4-R388 induced MT1-MMP phosphorylation and endosomal stabilization, and surprisingly, the increased MT1-MMP in return enhanced FGFR4-R388 autophosphorylation. A phosphorylation-defective MT1-MMP was stabilized on the cell surface, where it induced simultaneous FGFR4-R388 internalization and dissociation of cell–cell junctions. In contrast, the alternative FGFR4-G388 variant down-regulated MT1-MMP, and the overexpression of MT1-MMP and particularly its phosphorylation-defective mutant vice versa induced FGFR4-G388 degradation. These results provide a mechanistic basis for FGFR4-R388 function in cancer invasion.
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| 26. |
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