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| 4. |
- Acar-Denizli, N., et al.
(författare)
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Systemic phenotype related to primary Sjögren's syndrome in 279 patients carrying isolated anti-La/SSB antibodies
- 2020
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Ingår i: Clinical and Experimental Rheumatology. - : CLINICAL & EXPER RHEUMATOLOGY. - 0392-856X .- 1593-098X. ; 38:4; Suppl. 126, s. S85-S94
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Tidskriftsartikel (refereegranskat)abstract
- Objective: To evaluate the systemic phenotype associated with the presence of isolated anti-La/SSB antibodies in a large international registry of patients with primary Sjogren's syndrome (pSS) fulfilling the 2002 classification criteria.Methods: The Big Data Sjogren Project Consortium is an international, multicentre registry created in 2014. Baseline clinical information from leading centres on clinical research in SS of the 5 continents was collected. Combination patterns of anti-Ro/SSA-La/SSB antibodies at the time of diagnosis defined the following four immunological phenotypes: double positive (combined Ro/SSA and La/SSB,) isolated anti-Ro/SSA, isolated anti-La/SSB, and immunonegative.Results: The cohort included 12,084 patients (11,293 females, mean 52.4 years) with recorded ESSDAI scores available. Among them, 279 (2.3%) had isolated anti-La/SSB antibodies. The mean total ESSDAI score at diagnosis of patients with pSS carrying isolated anti-La/SSB was 6.0, and 80.4% of patients had systemic activity (global ESSDAI score >= 1) at diagnosis. The domains with the highest frequency of active patients were the biological (42.8%), glandular (36.8%) and articular (31.2%) domains. Patients with isolated anti-La/ SSB showed a higher frequency of active patients in all ESSDAI domains but two (articular and peripheral nerve) in comparison with immune-negative patients, and even a higher absolute frequency in six clinical ESSDAI domains in comparison with patients with isolated anti-Ro/SSA. In addition, patients with isolated anti-La/SSB showed a higher frequency of active patients in two ESSDAI domains (pulmonary and glandular) with respect to the most active immunological subset (double-positive antibodies). Meanwhile, systemic activity detected in patients with isolated anti-La/SSB was overwhelmingly low. Even in ESSDAI domains where patients with isolated anti-La/SSB had the highest frequencies of systemic activity (lymphadenopathy and muscular), the percentage of patients with moderate or high activity was lower in comparison with the combined Ro/SSA and La/SSB group.Conclusion: Patients carrying isolated La/SSB antibodies represent a very small subset of patients with a systemic SS phenotype characterised by a significant frequency of active patients in most clinical ESSDAI domains but with a relative low frequency of the highest severe organ-specific involvements. Primary SS still remains the best clinical diagnosis for this subset of patients.
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| 5. |
- Alftberg, Åsa, et al.
(författare)
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Fenomenologins möjligheter i etnologin: Rapport från en nationell workshop.
- 2011
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Rapport (övrigt vetenskapligt/konstnärligt)abstract
- Fredag den 17 december 2010 genomfördes en nationell workshop kring fenomenologins vara i etnologin vid Institutionen för kulturvetenskaper, Lunds universitet. Denna rapport är ett resultat från workshopen och skall ses som ett försök att intensifiera den diskussion som var på workshopen. Etnologin har tagit inspiration från fenomenologin och i denna filosofi har ämnet funnit teorier och metodologier för att utveckla kulturanalysen. Ibland har inspirationen kommit från de fenomenologiska klassikerna - Martin Heidegger, Maurice Merleau-Ponty, Simone de Beauvoir – andra gånger har inspirationen varit antropologer och sociologer som har varit starkt färgade av fenomenologin. Idag vågar vi påstå att det finns en så stor etnologisk diskussion och användning av denna filosofi att vi kan prata om en etnologisk tolkning av fenomenologi.
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| 6. |
- Bahramian, Nahid, et al.
(författare)
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Fatty acids induce increased granulocyte macrophage-colony stimulating factor secretion through protein kinase C-activation in THP-1 macrophages.
- 2004
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Ingår i: Lipids. - 0024-4201. ; 39:3, s. 243-9
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Tidskriftsartikel (refereegranskat)abstract
- Insulin resistance and type 2 diabetes are associated with elevated circulating levels of nonesterified FA (NEFA) and lipoprotein remnants. The dyslipidemia is an important contributor to the excess arterial disease associated with insulin resistance and type 2 diabetes, but the mechanisms involved are elusive. In the present study we examined the effect of NEFA on macrophages. For this purpose, we utilized human macrophages, prepared by treating THP-1 monocytes with phorbol ester. We found that albumin-bound NEFA at physiological levels increase the secretion of granulocyte macrophage-colony stimulating factor (GM-CSF) by the THP-1 macrophages in a dose-dependent manner. The effect was registered as an increase in mRNA, and the amount of GM-CSF secreted correlated with the accumulation of TAG and DAG in the cell. The NEFA-induced rise in GM-CSF appeared to be mediated by activation of protein kinase C, probably acting on extracellular signal-regulated kinases 1 and 2 and being calcium dependent. We speculate that increased secretion of GM-CSF by resident macrophages in the intima exposed chronically to high levels of NEFA, such as those present in insulin resistance, may contribute to a proatherogenic response of arterial cells.
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| 7. |
- Berggren, Cathrine, et al.
(författare)
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Att utvärdera och använda resultaten – vilka avtryck ger bibliotekens utvärderingar i samhället?
- 2013
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Konferensbidrag (populärvet., debatt m.m.)abstract
- Det har blivit allt viktigare för biblioteken att kunna visa på sin betydelse i samhället. Mätetal och utvärderingar är nödvändiga instrument för att kunna jämföra satsningar på biblioteken med satsningar på andra områden. Men finns det en synergi mellan mätetalen och verksamhetens planer och mål? Hur mäter vi måluppfyllese i biblioteksverksamheten? Finns det kvalitetsindikatorer som vi har gemensamma oavsett bibliotekstyp? Hur kan biblioteksstatistiken användas? Vad gör bibliotekens utvärderingar för avtryck i beslutsprocessen? Vad gör bibliotekens utvärderingar för avtryck i samhället?För att svara på frågorna ovan har expertgruppen påbörjat ett arbete med att kartlägga hur svenska bibliotek arbetar med utvärdering. I det arbetet har vi bland annat sett vikten av ett gemensamt språk mellan bibliotekstyperna i arbetet med utvärderingar och kvalitetsutveckling. Gruppen kommer att under år 2014 publicera ett utvärderingsverktyg för bibliotek. Där kan bibliotek finna vägledning och stöd i valet av utvärderingsmetod, i genomförandet och i nyttjandet av utvärderingars resultat i syfte att utveckla och synliggöra biblioteksverksamheten.I det här papret presenterar vi syftet med vårt arbete och den metod vi har använt samt mer ingående berättar om hur vi har tänkt kring det verktyg som vi i slutet av papret ger en beskrivning av – ett verktyg som går under namnet "Utvärderingshjälpen".Vid konferensen kommer vi att presentera hur en årscykel kan se ut vid ett bibliotek som väljer att använda sig av utvärderingsarbete och systematiskt kvalitetsutveckling i nära relation till måluppfyllelse och till dialog med beslutsfattare samt omgivande samhälle. Vi presenterar också hur "Utvärderingshjälpen" skall kunna användas som stöd i det arbetet.
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| 8. |
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| 9. |
- Bjursell, Mikael, 1977, et al.
(författare)
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Opposing effects of adiponectin receptors 1 and 2 on energy metabolism
- 2007
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Ingår i: DIABETES. - : American Diabetes Association. - 0012-1797 .- 1939-327X. ; 56:3, s. 583-593
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Tidskriftsartikel (refereegranskat)abstract
- The adipocyte-derived hormone adiponectin regulates glucose and lipid metabolism and influences the risk for developing obesity, type 2 diabetes, and cardiovascular disease. Adiponectin binds to two different seven-transmembrane domain receptors termed AdipoR1 and AdipoR2. To study the physiological importance of these receptors, AdipoR1 gene knockout mice (AdipoR1−/−) and AdipoR2 gene knockout mice (AdipoR2−/−) were generated. AdipoR1−/− mice showed increased adiposity associated with decreased glucose tolerance, spontaneous locomotor activity, and energy expenditure. However, AdipoR2−/− mice were lean and resistant to high-fat diet–induced obesity associated with improved glucose tolerance and higher spontaneous locomotor activity and energy expenditure and reduced plasma cholesterol levels. Thus, AdipoR1 and AdipoR2 are clearly involved in energy metabolism but have opposing effects.
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| 10. |
- Brito-Zeron, P., et al.
(författare)
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How immunological profile drives clinical phenotype of primary Sjögren's syndrome at diagnosis : analysis of 10,500 patients (Sjögren Big Data Project)
- 2018
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Ingår i: Clinical and Experimental Rheumatology. - : CLINICAL & EXPER RHEUMATOLOGY. - 0392-856X .- 1593-098X. ; 36:3, s. S102-S112
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Tidskriftsartikel (refereegranskat)abstract
- Objective: To evaluate the influence of the main immunological markers on the disease phenotype at diagnosis in a large international cohort of patients with primary Sjogren's syndrome (SjS).Methods: The Big Data Sjogren Project Consortium is an international, multicentre registry created in 2014. As a first step, baseline clinical information from leading centres on clinical research in SjS of the 5 continents was collected. The centres shared a harmonised data architecture and conducted cooperative online efforts in order to refine collected data under the coordination of a big data statistical team. Inclusion criteria were the fulfillment of the 2002 classification criteria. Immunological tests were carried out using standard commercial assays.Results: By January 2018, the participant centres had included 10,500 valid patients from 22 countries. The cohort included 9,806 (93%) women and 694 (7%) men, with a mean age at diagnosis of primary SjS of 53 years, mainly White (78%) and included from European countries (71%). The frequency of positive immunological markers at diagnosis was 79.3% for ANA, 73.2% for anti-Ro, 48.6% for RF, 45.1% for anti-La, 13.4% for low C3 levels, 14.5% for low C4 levels and 7.3% for cryoglobulins. Positive autoantibodies (ANA, Ro, La) correlated with a positive result in salivary gland biopsy, while hypocomplementaemia and especially cryoglobulinaemia correlated with systemic activity (mean ESSDAI score of 17.7 for ctyoglobulins, 11.3 for low C3 and 9.2 for low C4, in comparison with 3.8 for negative markers). The immunological markers with a great number of statistically-significant associations (p<0.001) in the organ-by-organ ESSDAI evaluation were cryoglobulins (9 domains), low C3 (8 domains), anti-La (7 domains) and low C4 (6 domains).Conclusion: We confirm the strong influence of immunological markers on the phenotype of primary SjS at diagnosis in the largest multi-ethnic international cohort ever analysed, with a greater influence for cryoglobulinaemic-related markers in comparison with Ro/La autoantibodies and ANA. Immunological patterns play a central role in the phenotypic expression of the disease already at the time of diagnosis, and may guide physicians to design a specific personalised management during the follow-up of patients with primary SjS.
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| 11. |
- Cardell, Lars-Olaf, et al.
(författare)
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TOTALL: high cost of allergic rhinitis-a national Swedish population-based questionnaire study.
- 2016
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Ingår i: NPJ primary care respiratory medicine. - : Springer Science and Business Media LLC. - 2055-1010. ; 26
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Tidskriftsartikel (refereegranskat)abstract
- Allergic rhinitis is a global illness with a well-recognised impact on quality of life and work performance. Comparatively little is known about the extent of its economic impact on society. The TOTALL study estimates the total cost of allergic rhinitis using a sample representing the entire Swedish population of working age. A questionnaire focused on allergic rhinitis was mailed out to a random population of Swedish residents, aged 18-65 years. Health-care contacts, medications, absenteeism (absence from work) and presenteeism (reduced working capacity at work) were assessed, and the direct and indirect costs of allergic rhinitis were calculated. Medication use was evaluated in relation to the ARIA guidelines. In all, 3,501 of 8,001 (44%) answered the questionnaire, and 855 (24%) of these reported allergic rhinitis. The mean annual direct and indirect costs because of allergic rhinitis were €210.3 and €750.8, respectively, resulting in a total cost of €961.1 per individual/year. Presenteeism represented 70% of the total cost. Antihistamines appear to be used in excess in relation to topical steroids, and the use of nasal decongestants was alarmingly high. The total cost of allergic rhinitis in Sweden, with a population of 9.5 million, was estimated at €1.3 billion annually. These unexpectedly high costs could be related to the high prevalence of disease, in combination with the previously often underestimated indirect costs. Improved adherence to guidelines might ease the economic burden on society.
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| 12. |
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| 13. |
- Fernell, Elisabeth, 1948, et al.
(författare)
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Serum levels of 25-hydroxyvitamin D in mothers of Swedish and of Somali origin who have children with and without autism.
- 2010
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Ingår i: Acta Paediatrica. - : Wiley. - 0803-5253 .- 1651-2227. ; 99:5, s. 743-747
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Tidskriftsartikel (refereegranskat)abstract
- Aim: To analyse serum levels of 25-hydroxyvitamin D in mothers of Somali origin and those of Swedish origin who have children with and without autism as there is a growing evidence that low vitamin D impacts adversely on brain development. Method: Four groups of mothers were invited to participate; 20 with Somali origin with at least one child with autism, 20 with Somali origin without a child with autism, 20 of Swedish origin with at least one child with autism and 20 with Swedish origin without a child with autism. Two blood samples were collected from each individual; during autumn and spring. Results: Between 12 and 17 mothers from the different groups accepted to participate, both groups of mothers of Somali origin had significantly lower values of 25-hydroxyvitamin D compared with Swedish mothers. The difference of 25-hydroxyvitamin D between mothers of Somali origin with and without a child with autism was not significant. Conclusion: Our findings of low vitamin D levels in Somali women entail considerable consequences in a public health perspective. The observed tendency, i.e. the lowest values in mothers of Somali origin with a child with autism was in the predicted direction, supporting the need for further research of vitamin D levels in larger samples of Somali mothers of children with and without autism.
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| 14. |
- Hallström, Inger, et al.
(författare)
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Sjuksköterskans roll i smärtbehandling
- 2001
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Ingår i: Smärta hos barn och ungdomar. - 9789144014890 ; , s. 90-90
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Bokkapitel (övrigt vetenskapligt/konstnärligt)
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| 15. |
- Helldén, Leif, et al.
(författare)
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A prospective 5-year multicenter study of the Cresco implantology concept
- 2003
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Ingår i: International Journal of Prosthodontics. - 0893-2174 .- 1139-9791. ; 16:5, s. 554-562
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Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)abstract
- PURPOSE: The purpose of this study was to investigate the clinical and radiographic outcome of a prospective 5-year longitudinal multicenter test of a simplified implantology concept comprising an abut-ment-free implant system (Cresco) and a new method for fabrication of passively fitting superstructures, the Cresco Ti Precision method. MATERIALS AND METHODS: The tests were carried out at three different centers. Sixty partially or completely edentulous patients were restored by fixed implant-supported superstructures fabricated according to the Cresco Ti Precision method. In all, 215 Cresco implants were placed for the support of the superstructures. For various reasons, eight patients (13%) could not be followed through the 5 years. The radiographic measurements were recorded from radiographs exposed perpendicular to the implants. RESULTS: The initial implant failure rate (during the healing phase) was 2%, and the survival rate after loading was 98%. The mean peri-implant bone loss was 0.29 mm (SD 0.57). Of the measured sites, 71% showed a crestal bone loss of less than 0.5 mm. Very few mechanical complications were observed. This was attributed to the passively fitting superstructures. CONCLUSION: The results from the present multicenter test demonstrated that the abutment-free Cresco implantology concept is a reliable alternative method for implant-supported fixed prosthetic rehabilitation of edentulous and partially edentulous jaws.
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| 16. |
- Holmér, Gunnel, 1955-
(författare)
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Kosta glasbruk - en etnisk smältdegel? : Arbete och arbetskraftsinvandring 1943-1973
- 2009
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Licentiatavhandling (övrigt vetenskapligt/konstnärligt)abstract
- Avhandlingen fokuserar på arbetskraftsinvandringen till Kosta glasbruk under perioden 1943-1973. Den övergripande frågan är om svenskar och invandrare tilldelades likvärdiga arbetsuppgifter eller om diskriminering kopplad till arbetet förekommit. Vidare undersöks varför företaget rekryterade utländsk arbetskraft, vilka nationaliteter som var representerade, orsakerna bakom migrationen, invandrarnas kvalifikationer samt den lokala fackföreningens inställning till immigranterna. Studien bygger på arkivmaterial kompletterat med ett 40-tal intervjuer med såväl svenska som invandrade glasarbetare samt med tjänstemän.
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| 17. |
- Höglund, Evelina, et al.
(författare)
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A meal concept designed for older adults : small enriched meals including dessert
- 2018
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Ingår i: Food and Nutrition Research. ; :62
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Tidskriftsartikel (refereegranskat)abstract
- Background: The population of older adults is growing and many are at risk of disease-related malnutrition.This is a serious condition which increases the risk for other diseases and distress, human suffering and puts ahigh load on health care costs. Meal concepts tailored to suit the needs of older adults are required to decreasethe incidence of disease-related malnutrition.Objective: To evaluate sensory perception regarding a concept of small, protein and energy-enriched multicomponentmeals designed according to the nutritional needs of older adults.Design: A meal concept of small main courses with complementary desserts and protein and energy-enrichedproducts was evaluated using triangle tests, hedonic evaluation and focus group discussion. Enriched saucesand meals were compared to corresponding commercial products regarding appearance, taste, consistency andoverall acceptance.Results: The concept of a small main course with a complementary dessert was generally perceived as positiveby the target group. The acceptance scores for the enriched meals were generally lower than for the commercialmeals, mainly owing to the packaging of the enriched meals which required covering the food in sauce.Enriched sauces contained approximately 90% more protein than the commercial sauces. However, proteinenrichment affected the sensory properties of the sauces and they were perceived as thicker, creamier and lessflavour-intensive.Conclusions: A concept based on small, protein and energy-enriched meals supplemented with a dessert wasconsidered suitable for increasing the energy and protein intakes of older adults provided that the method ofenrichment ensures attractive sensorial properties.
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| 18. |
- Höglund, Evelina, et al.
(författare)
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A meal concept designed for older adults - Small, enriched meals including dessert.
- 2018
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Ingår i: Food & nutrition research. - : SNF Swedish Nutrition Foundation. - 1654-661X .- 1654-6628. ; 62
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Tidskriftsartikel (refereegranskat)abstract
- Background: The population of older adults is growing and many are at risk of disease-related malnutrition. This is a serious condition which increases the risk for other diseases and distress, human suffering and puts a high load on health care costs. Meal concepts tailored to suit the needs of older adults are required to decrease the incidence of disease-related malnutrition.Objective: To evaluate sensory perception regarding a concept of small, protein and energy-enriched multi-component meals designed according to the nutritional needs of older adults.Design: A meal concept of small main courses with complementary desserts and protein and energy-enriched products was evaluated using triangle tests, hedonic evaluation and focus group discussion. Enriched sauces and meals were compared to corresponding commercial products regarding appearance, taste, consistency and overall acceptance.Results: The concept of a small main course with a complementary dessert was generally perceived as positive by the target group. The acceptance scores for the enriched meals were generally lower than for the commercial meals, mainly owing to the packaging of the enriched meals which required covering the food in sauce. Enriched sauces contained approximately 90% more protein than the commercial sauces. However, protein enrichment affected the sensory properties of the sauces and they were perceived as thicker, creamier and less flavour-intensive.Conclusions: A concept based on small, protein and energy-enriched meals supplemented with a dessert was considered suitable for increasing the energy and protein intakes of older adults provided that the method of enrichment ensures attractive sensorial properties.
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| 19. |
- Höglund, Evevlina, et al.
(författare)
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Protein and energy enriched muffins designed for nutritional needs of older adults
- 2017
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Ingår i: Nutrition & Food Sceince. - 2474-767X. ; 2:4
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Tidskriftsartikel (refereegranskat)abstract
- Disease related malnutrition is a common problem among older adults which results in human suffering and high health care costs. One way to counteract this condition is to offer appetizing and nutritious food products adapted to the needs of older adults. However, macronutrient enrichment of food products is not straight forward as it affects flavour and texture properties. In this study, muffins were fortified with rape seed oil and whey powder to reach increased fat and/or protein content and the nutritional value, sensory properties and physical parameters were investigated. The results showed that ingredients could be added to significantly increase the energy and protein density, but enrichment affected on appearance and other sensory properties. Extra fat made the muffins flat and moist with a smooth and fatty mouth feel, while whey powder addition resulted in high/pointy muffins with shiny appearance, hard texture and altered flavours. For co-addition of fat and protein the effects of added protein dominated. The specific role of muffin/cake batter compounds, and their transformation during the baking process, needs to be further investigated. Hence, more research is needed in order to offer older adult appealing foods with high energy and nutrient density.
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| 20. |
- Höglund, Evevlina, et al.
(författare)
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Protein and energy enriched muffins designed for nutritional needs of older adults
- 2017
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Ingår i: Nutrition & Food Sceince. - 2474-767X. ; 2:4
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Tidskriftsartikel (refereegranskat)abstract
- Disease related malnutrition is a common problem among older adults which results in human suffering and high health care costs. One way to counteract this condition is to offer appetizing and nutritious food products adapted to the needs of older adults. However, macronutrient enrichment of food products is not straight forward as it affects flavour and texture properties. In this study, muffins were fortified with rape seed oil and whey powder to reach increased fat and/or protein content and the nutritional value, sensory properties and physical parameters were investigated. The results showed that ingredients could be added to significantly increase the energy and protein density, but enrichment affected on appearance and other sensory properties. Extra fat made the muffins flat and moist with a smooth and fatty mouth feel, while whey powder addition resulted in high/pointy muffins with shiny appearance, hard texture and altered flavours. For co-addition of fat and protein the effects of added protein dominated. The specific role of muffin/cake batter compounds, and their transformation during the baking process, needs to be further investigated. Hence, more research is needed in order to offer older adult appealing foods with high energy and nutrient density.
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| 21. |
- Jin, Y. K., et al.
(författare)
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A Dual-Promoter Gene Orchestrates the Sucrose-Coordinated Synthesis of Starch and Fructan in Barley
- 2017
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Ingår i: Molecular Plant. - : Elsevier BV. - 1674-2052 .- 1752-9867. ; 10:12, s. 1556-1570
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Tidskriftsartikel (refereegranskat)abstract
- Sequential carbohydrate synthesis is important for plant survival because it guarantees energy supplies for growth and development during plant ontogeny and reproduction. Starch and fructan are two important carbohydrates in many flowering plants and in human diets. Understanding this coordinated starch and fructan synthesis and unraveling how plants allocate photosynthates and prioritize different carbohydrate synthesis for survival could lead to improvements to cereals in agriculture for the purposes of greater food security and production quality. Here, we report a system from a single gene in barley employing two alternative promoters, one intronic/exonic, to generate two sequence-overlapping but functionally opposing transcription factors, in sensing sucrose, potentially via sucrose/glucose/fructose/trehalose 6-phosphate signaling. The system employs an autoregulatory mechanism in perceiving a sucrose-controlled trans activity on one promoter and orchestrating the coordinated starch and fructan synthesis by competitive transcription factor binding on the other promoter. As a case in point for the physiological roles of the system, we have demonstrated that this multitasking system can be exploited in breeding barley with tailored amounts of fructan to produce healthy food ingredients. The identification of an intron/exon-spanning promoter in a hosting gene, resulting in proteins with distinct functions, adds to the complexity of plant genomes. ERYSTWYTH, WALES, V123, P453
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| 22. |
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| 23. |
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| 24. |
- LEMAITRE, D, et al.
(författare)
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ARTEMIS-2 - AN APPLICATION DEVELOPMENT EXPERIMENT WITH THE HELIOS ENVIRONMENT
- 1994
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Ingår i: COMPUTER METHODS AND PROGRAMS IN BIOMEDICINE. - : ELSEVIER SCI PUBL IRELAND LTD. - 0169-2607. ; 45, s. S127-S138
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Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)abstract
- A medical application is a highly complex system that embraces many data types and a very large number of data processing functions and methods. The development of integrated software engineering environments has deeply changed the conception of applicati
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| 25. |
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| 26. |
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| 27. |
- Lundtoft, Christian, et al.
(författare)
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Function of multiple sclerosis-protective HLA class I alleles revealed by genome-wide protein-quantitative trait loci mapping of interferon signalling
- 2020
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Ingår i: PLOS Genetics. - : Public Library of Science (PLoS). - 1553-7390 .- 1553-7404. ; 16:10
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Tidskriftsartikel (refereegranskat)abstract
- Interferons (IFNs) are cytokines that are central to the host defence against viruses and other microorganisms. If not properly regulated, IFNs may contribute to the pathogenesis of inflammatory autoimmune, or infectious diseases. To identify genetic polymorphisms regulating the IFN system we performed an unbiased genome-wide protein-quantitative trait loci (pQTL) mapping of cell-type specific type I and type II IFN receptor levels and their responses in immune cells from 303 healthy individuals. Seven genome-wide significant (p < 5.0E-8) pQTLs were identified. Two independent SNPs that tagged the multiple sclerosis (MS)-protective HLA class I alleles A*02/A*68 and B*44, respectively, were associated with increased levels of IFNAR2 in B and T cells, with the most prominent effect in IgD–CD27+ memory B cells. The increased IFNAR2 levels in B cells were replicated in cells from an independent set of healthy individuals and in MS patients. Despite increased IFNAR2 levels, B and T cells carrying the MS-protective alleles displayed a reduced response to type I IFN stimulation. Expression and methylation-QTL analysis demonstrated increased mRNA expression of the pseudogene HLA-J in B cells carrying the MS-protective class I alleles, possibly driven via methylation-dependent transcriptional regulation. Together these data suggest that the MS-protective effects of HLA class I alleles are unrelated to their antigen-presenting function, and propose a previously unappreciated function of type I IFN signalling in B and T cells in MS immune-pathogenesis.Author summaryGenetic association studies have been very successful in identifying disease-associated single nucleotide polymorphisms (SNPs), but it has been challenging to define the molecular mechanisms underlying these associations. As interferons (IFNs) have a central role in the immune system, we hypothesized that some of the SNPs associated to immune-mediated diseases would affect the IFN system. By combining genetic data with characterization of interferon receptor levels and their responses on the protein level in immune cells from 303 genotyped healthy individuals, we show that two SNPs tagging the HLA class I alleles A*02/A*68 and B*44 are associated with a decreased response to type I IFN stimulation in B cells and T cells. Notably, both HLA-A*02 and HLA-B*44 confer protection from developing multiple sclerosis (MS), which is a chronic inflammatory neurologic disease. In addition to suggesting a pathogenic role of enhanced type I interferon signalling in B cells and T cells in MS, our data emphasize the fact that genetic associations in the HLA locus can affect functions not directly associated to antigen presentation, which conceptually may be important for other diseases genetically associated to the HLA locus.
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| 28. |
- Lustig, Florentina, 1948, et al.
(författare)
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Alternative splicing determines the binding of platelet-derived growth factor (PDGF-AA) to glycosaminoglycans.
- 1996
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Ingår i: Biochemistry. - : American Chemical Society (ACS). - 0006-2960 .- 1520-4995. ; 35:37, s. 12077-85
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Tidskriftsartikel (refereegranskat)abstract
- We have shown previously that the platelet-derived growth factor (PDGF) and a synthetic oligopeptide, corresponding to the basic carboxyl-terminal amino acid extension of the long PDGF-A isoform, bind to heparin. Here, we have expressed the long (rA125) and the short (rA109) variants of PDGF A-chains in Escherichia coli and produced the functional homodimers. Surface plasmon resonance analyses showed that while the dimeric rA125 bound with high affinity to low molecular weight heparin, the rA109, lacking the basic extension, did not. This strongly indicated that high affinity binding is due to the carboxyl-terminal extension. Investigations of kinetics and thermodynamics suggested an allosteric binding mechanism. Thus, dimeric rA125 contains two equivalent binding sites. Following low affinity binding of heparin to one binding site, the dimer undergoes a conformational change, increasing the affinity for heparin about 40 times. This positive cooperativity requires the basic amino acid extension in both monomers of the dimeric PDGF molecule. Thermodynamics of the reaction, showing an entropy-driven endothermic process, suggest the involvement of hydrophobic interactions in this rearrangement. Three amino acids in the basic carboxyl-terminal extension were essential for the interaction: the basic residues Arg111 and Lys116, and the polar Thr125. We also found that other glycosaminoglycan species, corresponding to those produced by human arterial smooth muscle cells, bound to dimeric rA125 and that heparan sulfate showed the highest affinity.
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| 29. |
- Olsson, Gunnel, et al.
(författare)
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Kinetics of coal desulphurization by Acidianus brierleyi
- 1993
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Ingår i: Chemical Engineering & Technology. - : Wiley. - 1521-4125 .- 0930-7516. ; 16:3, s. 180-185
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Tidskriftsartikel (refereegranskat)abstract
- Acidianus brierleyi was demonstrated to remove pyritic sulphur from coal. A. brierleyi was also found capable of catalyzing the removal of what is normally reported as organic sulphur from coal 171US34. A kinetic analysis was performed by assuming a first order reaction. The first order kinetics allowed a comparison with literature data for Thiobacillus ferrooxidans. Also, formation of jarosite was taken into account in the analysis. The simple first order kinetics was observed to fit the data on removal of sulphur satisfactorily.
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| 30. |
- Olsson, Gunnel
(författare)
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Mellan Rum. En studie i fysisk och mental utveckling av kommunikation med utgångspunkt i en mellanstadieklass
- 2003
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Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- A Gap: A study in physical and mental development of communication based on a primary school class The primary aim of the dissertation is to describe the role played by body, emotions, and movement in the everyday life of school. This includes both physical and verbal linguistic movements. In theoretical terms the analyses are mainly based on the French phenomenologist Maurice Merleau-Ponty’s ideas about lived existence. With that aim I have conducted an intensive field study in a 5th grade in Sweden. The norms that apply to who one should be as a person are largely learned during ordinary working days. With this focus I simultaneously concentrated my attention on the pupils for whom today’s school generates difficulties. My other aim was to elucidate the question of how today’s situation could have arisen. I wanted to follow the changed role of pupil, partly by studying, via the history of school gymnastics, how pupils learned to open their bodies both to become expressive and to receive impressions, partly by studying how psychology has in its own way opened the senses, and how these two together have changed the prevailing epistemology. What I saw in the classroom was in large measure a matter of mutual communication between bodily movement and mental activity. My intention was to investigate ideas that create social and cultural effects. I found that not only gymnastics instruction but also psychology belonged here. Psychoanalysis seemed particularly interesting as a liberation project. During my work on the dissertation it became increasingly clear how psychological technique and theory have been incorporated in our everyday knowledge. This is not least interwoven in the pedagogics of school. A special motivation in my choice of topic came from the fact that I found the contemporary debate about school superficial and, as regards the lower and intermediate levels at least, unfair and misleading. I wanted to add some nuance to that debate by showing the historical growth of today’s situation in school as a conscious ideology with roots going back further than we often imagine. As an unexpected side effect I caught sight of an interaction between male and female, whereby a female attitude undermined a male one.
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| 31. |
- Olsson, Gunnel, et al.
(författare)
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Microbial Desulfurization of Coal and Oxidation of Pure Pyrite by T. ferrooxidans and Acidianus brierleyi
- 1995
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Ingår i: Journal of Industrial Microbiology. - 0169-4146. ; 14:5, s. 420-423
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Tidskriftsartikel (refereegranskat)abstract
- Thiobacillus ferrooxidans and Acidianus brierleyi were capable of oxidizing pure pyrite as well as oxidizing sulfur in coal. First order reactions were assumed in the kinetic analysis performed. For oxidation of pure pyrite the rate constant was higher for A. brierleyi than for T. ferrooxidans. For sulfur removal from coal the values of the rate constants were comparable far the two microorganisms.
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| 32. |
- Olsson, Louise
(författare)
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Hopp hos döende patienter med cancer i palliativ hemsjukvård
- 2011
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Licentiatavhandling (övrigt vetenskapligt/konstnärligt)abstract
- Hope is meaningful and very important for people – even for people at the last days of their lives. Health professionals can sometimes describe hope in terms of hope for a cure, which may lead to feelings of hopelessness or empty hope for a patient with an incurable disease. In research, there is very little written about hope in the palliative home care context, even more so from the patient’s perspective. The aim of this project was to study how hope changes over time in cancer patients at the palliative phase, and to study the strategies used to cope with these changes. This was studied from the patients’ own perspective and in a home care context, but with the ambition of developing the results into a model or theory.Grounded Theory was the chosen research approach. Two sets of tape-recorded interviews were made with 11 patients in specialized palliative home care – a total of 20 interviews. The patients were also asked to write diaries about changes in their everyday feelings of hope within a 4-week period. Measurements of hope (HHI-S) and symptoms (ESAS) were also made at three occasions. Constant comparison of data was made during the analysis.The results showed that patients’ hope changed over time. Hope was described as a process involving a glimmering core of hope untouched by external circumstances (Study I). Hope was described in different processes, creation of a convinced hope, creation of simulated hope, collection and maintaining moments of hope and gradually extinct hope. The dying patient’s hope can be described as a gradual and successive adaptation process. Strategies for maintaining life were expressed to preserve the meaning in life, to communicate with others about life and death, to involve "fellow travelers" and to change focus. The strategy of preparing for death involved; taking responsibility for the future and seeing possibilities of living on even after death (Study II). The strategies were parallel to, and dependent on each other. It is important that health professionals understand the patient’s own strategies for maintaining life/hope, as well as the patient’s own preparations for death, so that hope is never taken away despite preparations at the end of life.In summary, hope can be interpreted as a changing process over time – a process that can change quickly, but is based on an inviolable core that is not easily moved by external circumstances. It is important that the health care personnel realize that patients simultaneously prepare for their deaths even as they try to maintain hope. Deeper understanding of what hope can mean for a patient can decrease the risk of hope being taken away during one’s final days.
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| 33. |
- Olsson, Louise, et al.
(författare)
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Maintaining hope when close to death: insight from cancer patients in palliative home care
- 2010
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Ingår i: International Journal of Palliative Nursing. - London : Mark Allen group. - 1357-6321 .- 2052-286X. ; 16:12, s. 607-612
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Tidskriftsartikel (refereegranskat)abstract
- This article reports the social and psychological processes that 11 cancer patients receiving palliative home care used to keep their hope alive. Method: The patients described their ways of dealing with hope in interviews and diaries, and it was from these accounts that the processes were extracted, using a grounded theory methodology. Results: Two main processes emerged: maintaining life and preparing for death. When the patients focused on maintaining life, they tried to preserve its meaning and to communicate with others about life and death. They found it helpful to shift their focus and to involve fellow 'travellers' in their journey. To prepare for death was to take responsibility for the future and to see possibilities of living on even after death. Conclusion: This paper revealed two parallel and interdependent processes used by patients in the palliative care phase to maintain hope and respond to living close to death.
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| 34. |
- Olsson, Louise, 1957-, et al.
(författare)
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The Glimmering Embers : Experiences of hope among cancer patients in palliative home care
- 2011
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Ingår i: Palliative & Supportive Care. - : Cambridge University Press. - 1478-9515 .- 1478-9523. ; 9:1, s. 43-54
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Tidskriftsartikel (refereegranskat)abstract
- The experience of hope among cancer patients in palliative care is important knowledge for health care providers, but research is sparse. The aim of this paper was to explore how cancer patients admitted to palliative home care experienced the significance of hope and used hope during six weeks throughout the last phase of their life and to assess their symptoms and hope status during six weeks throughout the last phase of their lives.Eleven adult patients with cancer participated in twenty interviews and completed seven diaries. The participants were recruited from two palliative care units in South-East of Sweden. The methods used were Grounded Theory (GT), and analysis was based on the constant comparative method.The core category, glimmering embers, was generated from four processes: (1) The creation of “convinced” hope, with a focus on positive events, formed in order to have something to look forward to; (2) The creation of “simulated hope”, including awareness of the lack of realism, but including attempts to believe in unrealistic reasons for hope; (3) The collection of and maintaining of moments of hope, expressing a wish to “seize the day” and hold on to moments of joy and pleasure; and (4) “Gradually extinct” hope, characterized by a lack of energy and a sense of time running out. The different processes of hope helped the patients to continue to live when they were close to death. Hope should be respected and understood by the professionals giving them support.
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| 35. |
- Olsson Lönn, Eva M., 1967-
(författare)
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Thérèse Raquin d’Émile Zola : Répétitions lexicales, réseaux sémantiques et leurs traductions suédoises
- 2013
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Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- The subject of this thesis is Emile Zola’s novel Thérèse Raquin (1867). The principal aim is to examine lexical repetitions and their importance for semantic networks. The thesis studies the use of the noun cou and certain of its co-occurrences, as well as the use of colours and their derivatives. Employing the methods of Greimas and Rastier, the study is based upon two analyses, one narratological and the other thematic, an approach which allows us not only to study the importance of lexical repetitions, but also to study another aspect of the writing, Zola’s various sources of inspiration. This approach aids in showing the stylistic profile of the novel from a new perspective. Our second aim concerns the Swedish translations of the text. The degree of equivalence of lexical repetitions and their transmission has been studied in three versions (Wilson, 1884, Bjurman, 1911, and Bouleau, 1953). Our analysis draws on Berman’s and Heldner’s ideas about the critical evaluation of translated literary texts.The results of this thesis show that Zola, in Thérèse Raquin, uses lexical repetitions to create a stylistic effect that not only draws inspiration from literary and artistic sources, but that is also inspired by real events of the time. These stylistic properties, such as the system of colour leitmotivs, must be conveyed in a translation that is to be considered faithful to the original. The findings of this study suggest that there is a dependency between two of the examined versions and that it would be desirable to produce a new Swedish translation of the novel, equivalent to Zola’s text.
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| 36. |
- Olsson, Sara, et al.
(författare)
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Poorer mental well-being and prior unmet need for mental healthcare: a longitudinal population-based study on men in Sweden
- 2021
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Ingår i: Archives of Public Health. - : Springer Science and Business Media LLC. - 0778-7367 .- 2049-3258. ; 79:1
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Tidskriftsartikel (refereegranskat)abstract
- Background: Depression and anxiety disorder contribute to a significant part of the disease burden among men, yet many men refrain from seeking care or receive insufficient care when they do seek it. This is plausibly associated with poorer mental well-being, but there is a lack of population-based research. This study investigated 1) if men who had refrained from seeking mental healthcare at any time in life had poorer mental well-being than those who sought care, 2) if those who had sought care but perceived it as insufficient had poorer mental well-being than those who had perceived care as sufficient, and 3) if these differences persisted after 1year. Methods: This longitudinal study used questionnaire data from a population-based sample of 1240 men, aged 19–64 years, in Sweden. Having refrained from seeking mental healthcare, or perceiving the care as insufficient, at any time in life, was assessed in a questionnaire, 2008. Current mental well-being was assessed in 2008 and 2009 using mean scores on the WHO (Ten) Well-being Index. Lower scores indicate poorer mental well-being. Group differences were calculated using t-tests and multivariable linear regression analysis. Results: Of the men who had perceived a need for mental healthcare, 37% had refrained from seeking such care. They had lower mental well-being scores in 2008, compared to those who sought care. Of those seeking care, 29% had perceived it as insufficient. They had lower mental well-being scores in 2008, compared to those who perceived the care as sufficient, but this was not statistically significant when controlling for potential confounders. There were no differences in mental well-being scores based on care-seeking or perceived care-sufficiency in 2009. Conclusions: This population-based study indicates that men who have previously refrained from seeking mental healthcare, or perceived the care as insufficient, have poorer mental well-being. However, the lack of differences at the one-year follow-up contradicts these results. The results highlight the need for larger longitudinal studies, measuring care-seeking within a more specified time frame. This should be combined with efforts to increase men’s mental healthcare-seeking and to provide mental healthcare that is perceived as sufficient.
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| 37. |
- Olsson, Sara, et al.
(författare)
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Unmet Need for Mental Healthcare in a Population Sample in Sweden: A Cross-Sectional Study of Inequalities Based on Gender, Education, and Country of Birth
- 2021
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Ingår i: Community Mental Health Journal. - : Springer Science and Business Media LLC. - 0010-3853 .- 1573-2789. ; 57:3, s. 470-481
-
Tidskriftsartikel (refereegranskat)abstract
- This cross-sectional study investigated if gender, education, and country of birth were associated with perceived need and unmet need for mental healthcare (i.e., refraining from seeking care, or perceiving care as insufficient when seeking it). Questionnaire and register data from 2008 were collected for 3987 individuals, aged 19-64 years, in a random population-based sample from western Sweden. Descriptive statistics and logistic regression analyses were used. Men were less likely to perceive a need for care than were women, even after adjusting for mental well-being. Men were also less likely to seek care and perceiving care as sufficient. People with secondary education were less likely to seek care than those with university education. There were no statistically significant differences based on country of birth. The observed gender and education-based inequalities increases our understanding of where interventions can be implemented. These inequalities in unmet need for mental healthcare should be targeted by the healthcare system.
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| 38. |
- Olsson, Viktoria, 1973-, et al.
(författare)
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Buckwheat – revival in a new foodscape
- 2021
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Ingår i: Nordic Association for Food Studies Workshop 2020. - Örebro. - 9789187789434 - 9789187789441 ; , s. 20-22
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Konferensbidrag (refereegranskat)abstract
- In July 1749, when visiting Scania, Carl Linnaeus, noted the snow-white fields of buck wheat (Linné, 1749/1999). In those days, the cultivation of buckwheat was widespread in the southern parts of Sweden. It was used for porridge and gruel, staple foods the common people (Olsson, u.å.) Buck wheat thrives on lean, calcareous soils and require very little input. During the 1930s, when industrial fertilizers were introduced, production and consumption fell sharply as buckwheat was outcompeted by more high-yielding crops like oats and potatoes.
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| 39. |
- Olsson, Viktoria, et al.
(författare)
-
Buckwheat – revival in a new foodscape
- 2021
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Konferensbidrag (övrigt vetenskapligt/konstnärligt)abstract
- In July 1749, when visiting Scania, Carl Linnaeus, noted the snow-white fields of buck wheat (Linné, 1749/1999). In those days, the cultivation of buckwheat was widespread in the southern parts of Sweden. It was used for porridge and gruel, staple foods the common people (Olsson, u.å.) Buck wheat thrives on lean, calcareous soils and require very little input. During the 1930s, when industrial fertilizers were introduced, production and consumption fell sharply as buckwheat was outcompeted by more high-yielding crops like oats and potatoes.
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| 40. |
- Ramos-Casals, Manuel, et al.
(författare)
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Childhood-onset of primary Sjögren's syndrome : phenotypic characterization at diagnosis of 158 children
- 2021
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Ingår i: Rheumatology. - : Oxford University Press. - 1462-0324 .- 1462-0332. ; 60:10, s. 4558-4567
-
Tidskriftsartikel (refereegranskat)abstract
- Objectives: To characterize the phenotypic presentation at diagnosis of childhood-onset primary SS.Methods: The Big Data Sjögren Project Consortium is an international, multicentre registry using worldwide data-sharing cooperative merging of pre-existing clinical SS databases from the five continents. For this study, we selected those patients in whom the disease was diagnosed below the age of 19 years according to the fulfilment of the 2002/2016 classification criteria.Results: Among the 12 083 patients included in the Sjögren Big Data Registry, 158 (1.3%) patients had a childhood-onset diagnosis (136 girls, mean age of 14.2 years): 126 (80%) reported dry mouth, 111 (70%) dry eyes, 52 (33%) parotid enlargement, 118/122 (97%) positive minor salivary gland biopsy and 60/64 (94%) abnormal salivary US study, 140/155 (90%) positive ANA, 138/156 (89%) anti-Ro/La antibodies and 86/142 (68%) positive RF. The systemic EULAR Sjögren's syndrome disease activity index (ESSDAI) domains containing the highest frequencies of active patients included the glandular (47%), articular (26%) and lymphadenopathy (25%) domains. Patients with childhood-onset primary SS showed the highest mean ESSDAI score and the highest frequencies of systemic disease in 5 (constitutional, lymphadenopathy, glandular, cutaneous and haematological) of the 12 ESSDAI domains, and the lowest frequencies in 4 (articular, pulmonary, peripheral nerve and CNS) in comparison with patients with adult-onset disease.Conclusions: Childhood-onset primary SS involves around 1% of patients with primary SS, with a clinical phenotype dominated by sicca features, parotid enlargement and systemic disease. Age at diagnosis plays a key role in modulating the phenotypic expression of the disease.
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| 41. |
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| 42. |
- Retamozo, S., et al.
(författare)
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Influence of the age at diagnosis in the disease expression of primary Sjogren's syndrome : Analysis of 12,753 patients from the Sjogren Big Data Consortium
- 2021
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Ingår i: Clinical and Experimental Rheumatology. - : CLINICAL & EXPER RHEUMATOLOGY. - 0392-856X .- 1593-098X. ; 39:6, s. S166-S174
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Tidskriftsartikel (refereegranskat)abstract
- Objective. To analyse how the main components of the disease phenotype (sicca symptoms, diagnostic tests, immunological markers and systemic disease) can be driven by the age at diagnosis of primary Sjogren's syndrome (pSS).Methods. By January 2021, the participant centres had included 12,753 patients from 25 countries that fulfilled the 2002/2016 classification criteria for pSS. The age at diagnosis was defined as the time when the attending physician confirmed fulfilment of the criteria. Patients were clustered according to age at diagnosis. 50 clusters with more than 100 observations (from 27 to 76 years) were used to study the influence of the age at diagnosis in the disease expression.Results. There was a consistent increase in the frequency of oral dryness according to the age at diagnosis, with a frequency of <90% in patients diagnosed at the youngest ages and >95% in those diagnosed at the oldest ages. The smooth curves that best fitted a linear model were the frequency of dry mouth (adjusted R-2 0.87) and the frequency of abnormal oral tests (adjusted R-2 0.72). Therefore, for each 1-year increase in the age at diagnosis, the frequency of dry mouth increased by 0.13%, and the frequency of abnormal oral diagnostic tests by 0.11%. There was a consistent year-by-year decrease in the frequency of all autoantibodies and immunological markers except for cryoglobulins. According to the linear models, for each 1-year increase in the age at diagnosis, the frequency of a positive result decreased by 0.57% (for anti-Ro antibodies), 0.47% (for RF) and 0.42% (for anti-La antibodies). The ESSDAI domains which showed a more consistent decrease were glandular and lymph node involvement (for each 1-year increase in the age at diagnosis, the frequency of activity decreased by 0.18%), and constitutional, cutaneous, and haematological involvements (the frequency decreased by 0.09% for each 1-year increase). In contrast, other domains showed an ascending pattern, especially pulmonary involvement (for each 1-year increase in the age at diagnosis, the frequency of activity increased by 0.22%), and peripheral nerve involvement (the frequency increased by 0.09% for each 1-year increase).Conclusion. The influence of the age at diagnosis on the key phenotypic features of pSS is strong, and should be considered critical not only for designing a personalised diagnostic approach, but also to be carefully considered when analysing the results of diagnostic tests and immunological parameters, and when internal organ involvement is suspected at diagnosis.
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| 43. |
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| 44. |
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| 45. |
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| 46. |
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| 47. |
- Turunen Olsson, Pernilla, et al.
(författare)
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“It’s all connected!” : Nursing students’ experiences of an integrated case seminar
- 2016
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Ingår i: Innovations in Education and Teaching International. - : Routledge. - 1470-3300 .- 1470-3297. ; 53:2, s. 203-214
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Tidskriftsartikel (refereegranskat)abstract
- Traditionally, nursing students learn medical subjects and nursing separately, which makes it difficult to develop an integrated understanding. This study aimed to explore nursing students’ experiences of participating in a case seminar integrating medical and nursing sciences and if, and how, it contributed to their learning. A case seminar divided into a ‘laboratory session’ and a ‘follow-up session’ was developed. The case seminar was evaluated by group interviews and an open-ended questionnaire. Forty-four third-year nursing students agreed to participate. Themes of motivation, authenticity, professional development, collaboration and integration were identified. The case seminars help nursing students to integrate medical science with nursing science, which supports them in their professional development and role as registered nurses.
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| 48. |
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| 49. |
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| 50. |
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