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- Pansuriya, Twinkal C., et al.
(författare)
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Somatic mosaic IDH1 and IDH2 mutations are associated with enchondroma and spindle cell hemangioma in Ollier disease and Maffucci syndrome
- 2011
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Ingår i: Nature Genetics. - : Springer Science and Business Media LLC. - 1546-1718 .- 1061-4036. ; 43:12, s. 1256-1261
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Tidskriftsartikel (refereegranskat)abstract
- Ollier disease and Maffucci syndrome are non-hereditary skeletal disorders characterized by multiple enchondromas (Ollier disease) combined with spindle cell hemangiomas (Maffucci syndrome). We report somatic heterozygous mutations in IDH1 (c.394C>T encoding an R132C substitution and c.395G>A encoding an R132H substitution) or IDH2 (c.516G>C encoding R172S) in 87% of enchondromas (benign cartilage tumors) and in 70% of spindle cell hemangiomas (benign vascular lesions). In total, 35 of 43 (81%) subjects with Ollier disease and 10 of 13 (77%) with Maffucci syndrome carried IDH1 (98%) or IDH2 (2%) mutations in their tumors. Fourteen of 16 subjects had identical mutations in separate lesions. Immunohistochemistry to detect mutant IDH1 R132H protein suggested intraneoplastic and somatic mosaicism. IDH1 mutations in cartilage tumors were associated with hypermethylation and downregulated expression of several genes. Mutations were also found in 40% of solitary central cartilaginous tumors and in four chondrosarcoma cell lines, which will enable functional studies to assess the role of IDH1 and IDH2 mutations in tumor formation.
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- Mischke, M., et al.
(författare)
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Specific synbiotics in early life protect against diet-induced obesity in adult mice
- 2018
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Ingår i: Diabetes Obesity & Metabolism. - : Wiley. - 1462-8902. ; 20:6, s. 1408-1418
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Tidskriftsartikel (refereegranskat)abstract
- Aims: The metabolic state of human adults is associated with their gut microbiome. The symbiosis between host and microbiome is initiated at birth, and early life microbiome perturbation can disturb health throughout life. Here, we determined how beneficial microbiome interventions in early life affect metabolic health in adulthood. Methods: Postnatal diets were supplemented with either prebiotics (scGOS/lcFOS) or synbiotics (scGOS/lcFOS with Bifidobacterium breve M-16 V) until post-natal (PN) day 42 in a well-established rodent model for nutritional programming. Mice were subsequently challenged with a high-fat Western-style diet (WSD) for 8 weeks. Body weight and composition were monitored, as was gut microbiota composition at PN21, 42 and 98. Markers of glucose homeostasis, lipid metabolism and host transcriptomics of 6 target tissues were determined in adulthood (PN98). Results: Early life synbiotics protected mice against WSD-induced excessive fat accumulation throughout life, replicable in 2 independent European animal facilities. Adult insulin sensitivity and dyslipidaemia were improved and most pronounced changes in gene expression were observed in the ileum. We observed subtle changes in faecal microbiota composition, both in early life and in adulthood, including increased abundance of Bifidobacterium. Microbiota transplantation using samples collected from synbiotics- supplemented adolescent mice at PN42 to age-matched germ-free recipients did not transfer the beneficial phenotype, indicating that synbiotics-modified microbiota at PN42 is not sufficient to transfer long-lasting protection of metabolic health status. Conclusion: Together, these findings show the potential and importance of timing of synbiotic interventions in early life during crucial microbiota development as a preventive measure to lower the risk of obesity and improve metabolic health throughout life. RAHAMSEBERKEVELD, 2016, J NUTR SCI, V5
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- Nyokabi, Ndungu S., et al.
(författare)
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The role of power relationships, trust and social networks in shaping milk quality in Kenya
- 2023
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Ingår i: NJAS: Impact in Agricultural and Life Sciences. - : Taylor & Francis. - 2768-5241. ; 95:1
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Tidskriftsartikel (refereegranskat)abstract
- The objective of this study was to examine social networks in dairy value chains (DVCs) in Kenya and understand how DVC actors' power relationships and trust influence their behaviour regarding milk quality. We conducted a stakeholder analysis using the Net-Map tool in Laikipia, Nakuru and Nyandarua counties in Kenya. VisuaLyzer software was used to analyse the social networks. Thematic content analysis of the discussions, recorded during the mapping exercise, was undertaken using ATLAS.ti. Formal DVC had more actors and dense social networks characterised by vertical and horizontal integration, high levels of power asymmetries between actors, limited trust and short-term contractual arrangements. Informal DVC was characterised by fewer actors and less dense social networks, low levels of power asymmetries between actors and a high level of trust due to the existence of reciprocal personal relationships. Milk was perceived to be of higher quality in the formal value chain reflecting top-down enforcement of milk standards, bottom-up collective action, power asymmetries and contractual relationships. Poor milk quality management in the informal DVC underscores the need for powerful actors, e.g. regulatory agencies, and buyers such as processors, to influence other DVC actors' behavioural change. Understanding and leveraging DVC social networks and actors' power and addressing power asymmetries and enhancing trust between actors will increase compliance with milk quality standards. There is an urgent imperative to design policies and interventions which empower DVC actors, by providing economic incentives, enhancing their skills and knowledge and their access to infrastructure which facilitates milk quality improvement.
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- Nyokabi, Simon, et al.
(författare)
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Milk quality and hygiene : Knowledge, attitudes and practices of smallholder dairy farmers in central Kenya
- 2021
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Ingår i: Food Control. - : Elsevier. - 0956-7135 .- 1873-7129. ; 130
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Tidskriftsartikel (refereegranskat)abstract
- Milk production is an important livelihood source for smallholder dairy farmers in low-to-middle-income countries (LMICs) such as Kenya. However, milk quality and safety are a challenge due to unhygienic handling and non-adherence to food safety standards. The objective of this study was to investigate the knowledge, attitudes and adoption of milk quality and food safety practices by smallholder farmers in Kenya. Ten Focus Group Discussions (FGDs), involving 71 smallholder farmers, were held to collect qualitative data on knowledge, attitudes and practices (KAPs) of smallholder dairy farmers in Laikipia, Nakuru, and Nyandarua counties. Additionally, data were collected through a cross-sectional administered to 652 smallholder farming households. The results of the study revealed low knowledge level and negative attitudes towards respecting antibiotics treatment withdrawal periods, milk quality standards and food safety regulations. Farmers stated they had received low levels of training on milk quality and safety standards. The majority of farmers adopted animal health measures and hygienic measures such as hand washing and udder cleaning. However, unhygienic milking environments, the use of plastic containers, the use of untreated water, and lack of teat dipping compromised milk quality and safety. Currently, milk production, handling and consumption could expose actors along the dairy value chain to health risks. The adoption of milk quality and food safety practices was influenced by farmers' knowledge, socioeconomic characteristics, and choice of marketing channel. There is a need to improve farmers' knowledge and attitudes and implement hygienic control, disease control and antibiotic residue control practices in the milk production process to meet required milk quality and food safety standards. Awareness campaigns and training programmes for smallholder dairy farmers could foster behavioural change and lead to an improvement in milk quality in Kenya.
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- Nyokabi, Simon N., et al.
(författare)
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Milk quality along dairy farming systems and associated value chains in Kenya : An analysis of composition, contamination and adulteration
- 2021
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Ingår i: Food Control. - : Elsevier. - 0956-7135 .- 1873-7129. ; 119
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Tidskriftsartikel (refereegranskat)abstract
- Poor milk safety constitutes a persistent public health risk in Kenya. Poor milk composition, microbial contamination and adulteration is a constraint to dairy sector development. We hypothesise that variation in milk quality and safety depends on variation between farming systems. We argue that this variation between farming systems is associated with spatial location which affects the agro-ecological conditions and the avail-ability of labour and land. We used a spatial framework based on the distance to urban markets to distinguish the following farming systems: relatively intensive dairy systems in urban locations (UL), semi-intensive dairy systems in mid-rural locations (MRL) and extensive dairy systems in extreme rural locations (ERL). We aimed to investigate the variation in the quality of raw milk in these dairy farming systems and associated value chains in central Kenya. For this reason, we combined several methods such as participatory rural appraisal, participant observation, and milk physicochemical and microbiological analyses to collect data. Milk samples were collected at the informal and informal value chain nodes farms, informal collection centres, informal retailing centres including milk vending machines, and formal bulking centres where milk changes hands between value chain actors. Milk quality was compared to standards recommended by the Kenya Bureau of Standards (KeBS). There were no differences in the quality of raw milk between locations or between nodes. The overall milk physicochemical composition means (standard error) of the milk were within KeBS standards: fat 3.61 (0.05), protein 3.46 (0.06), solid-not fats 9.18 (0.04), density 1.031 (0.0002) and freezing point-0.597 (0.019). The protein percentage was below KeBS standards at all value chain nodes, except at the formal bulking node. There was significant contamination of milk samples: 16.7% of samples had added water, 8.8% had somatic cell count SCC above 300,000, 42.4% had E. coli, 47.9% had Pseudomonas spp., 3.3% had Staphylococcus spp. and 2.9% tested positive for brucellosis antibodies. Unsanitary milk handling practices were observed at farms and all value chains nodes. Milk physicochemical composition except for protein content meets the KeBS Standard. High levels of microbial contamination of milk pose a public health risk to consumers and show that urgent action is needed to improve milk quality.
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