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- Karlgren, M, et al.
(författare)
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Novel extrahepatic cytochrome P450s
- 2004
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Ingår i: TOXICOLOGY AND APPLIED PHARMACOLOGY. - 0041-008X. ; 197:3, s. 142-142
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Konferensbidrag (övrigt vetenskapligt/konstnärligt)
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- Pitarque, M, et al.
(författare)
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Transcriptional regulation of the human CYP2A6 gene
- 2005
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Ingår i: The Journal of pharmacology and experimental therapeutics. - : American Society for Pharmacology & Experimental Therapeutics (ASPET). - 0022-3565 .- 1521-0103. ; 313:2, s. 814-822
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Tidskriftsartikel (refereegranskat)
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- Åberg, Anna, et al.
(författare)
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Helicobacter pylori adapts to chronic infection and gastric disease via ph-responsive baba-mediated adherence
- 2017
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Ingår i: Cell Host and Microbe. - : Elsevier BV. - 1931-3128 .- 1934-6069. ; 21:3, s. 376-389
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Tidskriftsartikel (refereegranskat)abstract
- The BabA adhesin mediates high-affinity binding of Helicobacter pylori to the ABO blood group antigen-glycosylated gastric mucosa. Here we show that BabA is acid responsive-binding is reduced at low pH and restored by acid neutralization. Acid responsiveness differs among strains; often correlates with different intragastric regions and evolves during chronic infection and disease progression; and depends on pH sensor sequences in BabA and on pH reversible formation of high-affinity binding BabA multimers. We propose that BabA's extraordinary reversible acid responsiveness enables tight mucosal bacterial adherence while also allowing an effective escape from epithelial cells and mucus that are shed into the acidic bactericidal lumen and that bio-selection and changes in BabA binding properties through mutation and recombination with babA-related genes are selected by differences among individuals and by changes in gastric acidity over time. These processes generate diverse H. pylori subpopulations, in which BabA's adaptive evolution contributes to H. pylori persistence and overt gastric disease.
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| 26. |
- Ingelman-Sundberg, M, et al.
(författare)
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Human cytochrome P-450 (CYP) genes: a web page for the nomenclature of alleles
- 2001
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Ingår i: Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology. - 1055-9965. ; 10:12, s. 1307-1308
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Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)
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- Luis, Ana S., et al.
(författare)
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A single sulfatase is required to access colonic mucin by a gut bacterium
- 2021
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Ingår i: Nature. - : Springer Science and Business Media LLC. - 0028-0836 .- 1476-4687. ; 598, s. 332-337
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Tidskriftsartikel (refereegranskat)abstract
- Humans have co-evolved with a dense community of microbial symbionts that inhabit the lower intestine. In the colon, secreted mucus creates a barrier that separates these microorganisms from the intestinal epithelium(1). Some gut bacteria are able to utilize mucin glycoproteins, the main mucus component, as a nutrient source. However, it remains unclear which bacterial enzymes initiate degradation of the complex O-glycans found in mucins. In the distal colon, these glycans are heavily sulfated, but specific sulfatases that are active on colonic mucins have not been identified. Here we show that sulfatases are essential to the utilization of distal colonic mucin O-glycans by the human gut symbiont Bacteroides thetaiotaomicron. We characterized the activity of 12 different sulfatases produced by this species, showing that they are collectively active on all known sulfate linkages in O-glycans. Crystal structures of three enzymes provide mechanistic insight into the molecular basis of substrate specificity. Unexpectedly, we found that a single sulfatase is essential for utilization of sulfated O-glycans in vitro and also has a major role in vivo. Our results provide insight into the mechanisms of mucin degradation by a prominent group of gut bacteria, an important process for both normal microbial gut colonization(2) and diseases such as inflammatory bowel disease(3). A single sulfatase produced by a bacterium found in the human colon is essential for degradation of sulfated O-glycans in secreted mucus.
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- Bongo, A. K. S., et al.
(författare)
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Periodontal health in an indigenous Sami population in Northern Norway: a cross-sectional study
- 2020
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Ingår i: Bmc Oral Health. - : Springer Science and Business Media LLC. - 1472-6831. ; 20:1
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Tidskriftsartikel (refereegranskat)abstract
- Background The aim of the study was to describe prevalence, severity and distribution of periodontal disease as well as associated risk factors in an indigenous Sami population in Northern Norway, and to investigate differences between the indigenous Sami and the non-Sami population. Methods This cross-sectional study included data from the Dental Health in the North study (N = 2078; 18-75 years). Data on Ethnicity, household income, education, smoking habits, dental attendance, and tooth brushing habits were collected by a questionnaire. Periodontal conditions were assessed by clinical examination. A modified version of the new AAP/EFP classification system of periodontal disease was used to estimate the severity of periodontitis. Three stages were used: 'Non-severe periodontitis', 'Stage II', and stage 'III/IV'. Results Of the total study population 66.5% reported Sami affiliation. The total prevalence of periodontitis was 49.7%, with 20.1% in Stage III/IV, but no differences between Sami and non-Sami. When controlled for sex, age, education, smoking and dental attendance the Sami had higher probability of having more severe stages of periodontitis; Odds Ratio(Stage II) (OR) = 1.3; 95% CI: 1.1-1.7; and ORStage III/IV (OR) = 1.6; 95% CI: 1.1-2.2) compared to non-Sami. The Sami had higher prevalence of periodontal pocket depth (PD) >= 4 mm (t = 1.77; p < 0.001) and PD >= 6 mm (t = 1.08; p = 0.038) than the non-Sami. Conclusions The prevalence of periodontitis was high in communities in the core area of Sami settlement in Northern Norway, regardless of ethnicity. People with Sami ethnicity had more deep periodontal pockets and an increased odds of having severe stages of periodontitis. Future studies should address possible explaining factors behind the potential higher risk of having more severe periodontitis among indigenous people in Sami settlements.
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| 42. |
- Brustad, M., et al.
(författare)
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Oral health in the indigenous Sami population in Norway - the dental health in the North study
- 2020
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Ingår i: Acta Odontologica Scandinavica. - : Informa UK Limited. - 0001-6357 .- 1502-3850. ; 78:2, s. 98-108
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Tidskriftsartikel (refereegranskat)abstract
- Objective: This study aims at presenting the feasibility of using the public oral health clinics in indigenous Sami communities, as arena for a comprehensive data collection for population-based epidemiological oral health research among adults (age, 18-75 years) in a multi-ethnic setting. Material and methods: The study design was cross-sectional. The data collection was incorporated into the clinical procedure at six public dental clinics situated in the Administrative Area for the Sami Language in Finnmark County, Northern Norway, during 2013-2014. Both clinical- and questionnaire-data were collected. The quality of clinical data was thoroughly calibrated and validated. Results: Altogether, 2235 people participated in the study gave a crude response rate at 88.7%. In the final data sample (n = 2034), 56.9% were female. We constructed three ethnic groups (Sami, Mixed Sami/Norwegian and Norwegian). Altogether, 67.7% reported Sami or mixed Sami ethnicity. The internal validity of the clinical data was found to be satisfactory when assessed by comprehensive quality procedure, calibration and reliability assessments. Conclusion: This study design and method assessments provide solid documentation that public dental clinics are suitable as arenas for data collection in epidemiological oral health studies in the Sami population in this region.
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- Frygelius, J, et al.
(författare)
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The reproductive tissue specific cystatin subgroup of genes: expression during gonadal development in wildtype and testatin knockout animals
- 2007
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Ingår i: Sexual development : genetics, molecular biology, evolution, endocrinology, embryology, and pathology of sex determination and differentiation. - : S. Karger AG. - 1661-5433. ; 1:6, s. 363-372
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Tidskriftsartikel (refereegranskat)abstract
- <i>Testatin</i> has been implicated in fetal testis development due to its restricted expression in pre-Sertoli cells immediately after the onset of <i>Sry</i> gene expression. However,<i> testatin</i> knockout mice showed normal testis development and fertility. We investigated the spatial and temporal expression pattern of the <i>Cres/testatin</i> subgroup of genes, including the novel gene <i>Cstl1/Cres4</i>, in fetal mouse gonads and in adult testis, epididymis and ovary. The genes are related to the family 2 cystatins of protease inhibitors. Using real-time PCR and in situ hybridization we could show that 4 subgroup genes, <i>testatin, CstSC, CstTE-1/Cres3</i> and <i>Cres</i> are expressed in fetal testis. We also confirmed the expression of <i>testatin, CstE2, CstSC, CstTE-1/Cr</i>es3, <i>Cres, CstT</i> and <i>Cstl1/Cres4</i> in adult testis and <i>CstE2, CstTE-1/Cres3, Cres</i> and <i>CstE1/Cres2</i> in adult epididymis. In <i>testatin </i>knockout animals, the expression of <i>CstE2</i> was heavily downregulated in adult testis, but not in adult epididymis, compared to wildtype controls. In conclusion, an explanation for the lack of phenotype in <i>testatin</i> knockout mice could be functional redundancy with another member of the <i>Cres/testatin</i> subgroup. The most likely candidate/s would be <i>CstSC, CstTE-1/Cres3</i> or <i>Cres</i> as they are expressed in the fetal testicular tubules in early testis differentiation together with <i>testatin.</i>
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| 45. |
- Nestor, Gustav, et al.
(författare)
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A detailed picture of a protein-carbohydrate hydrogen-bonding network revealed by NMR and MD simulations
- 2021
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Ingår i: Glycobiology. - : Oxford University Press (OUP). - 0959-6658 .- 1460-2423. ; 31:4, s. 508-518
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Tidskriftsartikel (refereegranskat)abstract
- Cyanovirin-N (CV-N) is a cyanobacterial lectin with antiviral activity towards HIV and several other viruses. Here, we identify mannoside hydroxyl protons that are hydrogen bonded to the protein backbone of the CV-N domain B binding site, using NMR spectroscopy. For the two carbohydrate ligands Manα(1→2)ManαOMe and Manα(1→2) Manα(1→6)ManαOMe five hydroxyl protons are involved in hydrogen-bonding networks. Comparison with previous crystallographic results revealed that four of these hydroxyl protons donate hydrogen bonds to protein backbone carbonyl oxygens in solution and in the crystal. Hydrogen bonds were not detected between the side chains of Glu41 and Arg76 with sugar hydroxyls, as previously proposed for CV-N binding of mannosides. Molecular dynamics simulations of the CV-N/Manα(1→2)Manα(1→6)ManαOMe complex confirmed the NMR-determined hydrogen-bonding network. Detailed characterization of CV-N/mannoside complexes provides a better understanding of lectin-carbohydrate interactions and opens up to the use of CV-N and similar lectins as antiviral agents.
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| 46. |
- Nkambule, Comfort M., et al.
(författare)
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Efficient regioselective protection of myo-inositol via migration facile protecting group
- 2011
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Ingår i: Tetrahedron. - : Elsevier BV. - 0040-4020 .- 1464-5416. ; 67:3, s. 618-623
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Tidskriftsartikel (refereegranskat)abstract
- A cis-1,2-cyclohexanediol, 1,4,5,6-tetra-O-benzyl-myo-inositol, was selectively protected at the axial C2-hydroxyl via acid-mediated rearrangement of the corresponding 1,2-orthoacetate, or via the base-induced migration of a protecting group that had previously been easily installed with complete regioselectivity at the adjacent equatorial hydroxyl. Esters 4a-6a were synthesized in high yields (75-82%) while sulfonate 7a and silyl ether 8a were obtained in 85 and 31% yields, respectively. The migration of the esters induced by DBU results in equilibrium between regioisomers favouring the C2 protected isomer, but NaH induced migration of sulfonyl and silyl groups results in complete migration from equatorial to axial hydroxyl groups.
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| 47. |
- Olsson, Johan D. M., et al.
(författare)
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Investigations of glycosylation reactions with 2-N-acetyl-2N,3O-oxazolidinone-protected glucosamine donors
- 2008
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Ingår i: Journal of Organic Chemistry. - : American Chemical Society (ACS). - 0022-3263 .- 1520-6904. ; 73:18, s. 7181-7188
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Tidskriftsartikel (refereegranskat)abstract
- NIS/AgOTf-promoted glycosylations with ethyl 2,3-N,O-carbonyl-2-deoxy-1-thio-beta-D-glueopyranoside donors can be performed with either alpha- or beta-selectivity by tuning the reaction conditions. Small amounts of AgOTf (0.1 equiv) and short reaction times give beta-selectivity, whereas 0.4 equiv of AgOTf and prolonged reaction times afford alpha-linked products. NMR-monitored glycosylation and anomerization experiments show initial formation of exclusively the beta-linkage, which anomerizes, through an intramolecular mechanism involving an endocyclic C - O bond cleavage, to the alpha-linkage.
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