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- Blix Germundsson, Lisa, et al.
(författare)
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Participatory ex-ante impact assessment for interactive research and development in agriculture and food systems
- 2024
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Ingår i: Impact Assessment and Project Appraisal. - Abingdon : Taylor & Francis. - 1461-5517 .- 1471-5465. ; , s. 1-13
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Tidskriftsartikel (refereegranskat)abstract
- Drawing upon literature from both impact assessment of development projects and agricul-tural research, the aim of this article is to analyse the pilot testing of a new multi-dimensional assessment framework for defining and evaluating the societal impact of agricultural research and corresponding education. The research approach involves an action research effort of pilot testing in three case studies from three different countries. The framework assumes a systems view, understands impact as socially embedded, and adopts the concepts of contribution and productive interactions rather than cause-and-effect attribution. The emerging experiences include developing both project-specific and universal indicators; gauging impacts at different levels and sustainability dimensions; handling the issues of attribution and time frame; and the role of stakeholder involvement. The results have the potential to support the development of a new role for impact assessment, by enabling principal actors in research and higher educa-tion institutions to take responsibility for contributing to concrete and demonstrable sustain-ability changes in society. © 2024 The Author(s).
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| 2. |
- Niss, Frida, et al.
(författare)
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Polyglutamine expanded Ataxin-7 alters FUS localization and function in a SCA7 cell model
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Annan publikation (övrigt vetenskapligt/konstnärligt)abstract
- Polyglutamine (polyQ) diseases, such as Spinocerebellar ataxia type 7, are caused by the expansion of a CAG/polyglutamine repeat in a disease specific gene/protein. Misfolding and aggregation of the expanded protein can be observed in all polyQ disorders and sequestration of vital proteins into the aggregates formed have been suggested as a common pathological mechanism. FUS, an RNA binding protein, is frequently observed in polyglutamine aggregates. However, whether or not FUS disruption contributes to polyQ pathology is not clear.To address this question we used confocal microscopy, cell fractionation, filter traps and western blot, to study how FUS localization and function is affected by the SCA7 disease protein ataxin-7 (ATXN7). We found that aggregates formed by polyQ expanded ATXN7 were to a high degree also FUS positive and FUS re-distributed into the insoluble cell fraction together with mutant ATXN7. Moreover, a shift in abundance of FUS from the nucleus to the cytoplasm was observed and associated with altered levels of FUS regulated mRNAs in mutant ATXN7 expressing cells. However, some of the affected mRNAs are also regulated by the RNA binding protein TDP-43, which we could also show co-localized with ATXN7 aggregates using microscopy. Moreover, increased phosphorylation of serine 409/410 in TDP-43, which has been linked to TDP-43 neurotoxicity, could be observed in mutant ATXN7 expressing cells. Taken together, these findings lead us to conclude that disruption of FUS and also TDP-43 could potentially play a role in SCA7 pathology.
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