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- Petitfils, C., et al.
(författare)
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Identification of bacterial lipopeptides as key players in IBS
- 2023
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Ingår i: Gut. - : BMJ. - 0017-5749 .- 1468-3288. ; 72:5, s. 939-950
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Tidskriftsartikel (refereegranskat)abstract
- Objectives Clinical studies revealed that early-life adverse events contribute to the development of IBS in adulthood. The aim of our study was to investigate the relationship between prenatal stress (PS), gut microbiota and visceral hypersensitivity with a focus on bacterial lipopeptides containing gamma-aminobutyric acid (GABA). Design We developed a model of PS in mice and evaluated, in adult offspring, visceral hypersensitivity to colorectal distension (CRD), colon inflammation, barrier function and gut microbiota taxonomy. We quantified the production of lipopeptides containing GABA by mass spectrometry in a specific strain of bacteria decreased in PS, in PS mouse colons, and in faeces of patients with IBS and healthy volunteers (HVs). Finally, we assessed their effect on PS-induced visceral hypersensitivity. Results Prenatally stressed mice of both sexes presented visceral hypersensitivity, no overt colon inflammation or barrier dysfunction but a gut microbiota dysbiosis. The dysbiosis was distinguished by a decreased abundance of Ligilactobacillus murinus, in both sexes, inversely correlated with visceral hypersensitivity to CRD in mice. An isolate from this bacterial species produced several lipopeptides containing GABA including C14AsnGABA. Interestingly, intracolonic treatment with C14AsnGABA decreased the visceral sensitivity of PS mice to CRD. The concentration of C16LeuGABA, a lipopeptide which inhibited sensory neurons activation, was decreased in faeces of patients with IBS compared with HVs. Conclusion PS impacts the gut microbiota composition and metabolic function in adulthood. The reduced capacity of the gut microbiota to produce GABA lipopeptides could be one of the mechanisms linking PS and visceral hypersensitivity in adulthood.
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| 2. |
- Pujalte, V., et al.
(författare)
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Redefinition of the Ilerdian Stage (early Eocene)
- 2009
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Ingår i: Geologica Acta. - 1695-6133. ; 7:1-2, s. 177-194
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Tidskriftsartikel (refereegranskat)abstract
- The Ilerdian Stage was created by Hottinger and Schaub in 1960 to accommodate a significant phase in the evolution of larger foraminifera not recorded in the northern European basins, and has since been adopted by most researchers working on shallow marine early Paleogene deposits of the Tethys domain. One of the defining criteria of the stage is a major turnover of larger foraminifera, marked by the FO's of Alveolina vredenburgi (formerly A. cucumiformis) and Nummulites fraasi. There is now conclusive evidence that this turnover was coeval with the onset of the Carbon Isotope Excursion (CIE) and, consequently, with the Paleocene-Eocene (P-E) boundary, a temporal correspondence that reinforces the usefulness of the Ilerdian as a chronostratigraphic subdivision of the early Eocene in a regional context. However, in addition to the paleontological criteria, the definition of the Ilerdian was also based on the designation of two reference sections in the southern Pyrenees: Tremp (stratotype) and Campo (parastratotype). In both sections, the base of the stage was placed at the lowest marine bed containing A. vredenburgi specimens. Using the CIE as a correlation tool we demonstrate that these two marine beds occur at different chronological levels, being older in Campo than in Tremp. Further, we show that both beds are in turn younger than the lowest strata with Ilerdian larger foraminifera at the deep-water Ermua section in the Basque Basin (western Pyrenees). Since the age of stage boundaries must be the same everywhere, the choice of these stratotype sections was misleading, since in practice it resulted in the Ilerdian being used as a facies term rather than as a chronostratigraphic unit. To eliminate that conflict, and yet be respectful with established tradition, we propose to redefine the Ilerdian Stage following a procedure similar to the one used by the International Commission on Stratigraphy to establish global chronostratigraphic standards, namely: by using a "silver spike" to be placed in the Tremp section at the base of the Claret Conglomerate, a widespread lithological unit that in the Tremp-Graus Basin coincides with the onset of the CIE. The redefined regional Ilerdian Stage becomes thus directly correlatable to the lower part of the global Ypresian Stage, as currently defined by the International Commission on Stratigraphy.
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