| 1. |
|
|
| 2. |
|
|
| 3. |
- Lindh, Linnea, et al.
(författare)
-
Dye-sensitized solar cells based on Fe N-heterocyclic carbene photosensitizers with improved rod-like push-pull functionality
- 2021
-
Ingår i: Chemical Science. - : Royal Society of Chemistry (RSC). - 2041-6520 .- 2041-6539. ; 12:48, s. 16035-16053
-
Tidskriftsartikel (refereegranskat)abstract
- A new generation of octahedral iron(ii)-N-heterocyclic carbene (NHC) complexes, employing different tridentate C^N^C ligands, has been designed and synthesized as earth-abundant photosensitizers for dye sensitized solar cells (DSSCs) and related solar energy conversion applications. This work introduces a linearly aligned push-pull design principle that reaches from the ligand having nitrogen-based electron donors, over the Fe(ii) centre, to the ligand having an electron withdrawing carboxylic acid anchor group. A combination of spectroscopy, electrochemistry, and quantum chemical calculations demonstrate the improved molecular excited state properties in terms of a broader absorption spectrum compared to the reference complex, as well as directional charge-transfer displacement of the lowest excited state towards the semiconductor substrate in accordance with the push-pull design. Prototype DSSCs based on one of the new Fe NHC photosensitizers demonstrate a power conversion efficiency exceeding 1% already for a basic DSSC set-up using only the I−/I3−redox mediator and standard operating conditions, outcompeting the corresponding DSSC based on the homoleptic reference complex. Transient photovoltage measurements confirmed that adding the co-sensitizer chenodeoxycholic acid helped in improving the efficiency by increasing the electron lifetime in TiO2. Time-resolved spectroscopy revealed spectral signatures for successful ultrafast (<100 fs) interfacial electron injection from the heteroleptic dyes to TiO2. However, an ultrafast recombination process results in undesirable fast charge recombination from TiO2back to the oxidized dye, leaving only 5-10% of the initially excited dyes available to contribute to a current in the DSSC. On slower timescales, time-resolved spectroscopy also found that the recombination dynamics (longer than 40 μs) were significantly slower than the regeneration of the oxidized dye by the redox mediator (6-8 μs). Therefore it is the ultrafast recombination down to fs-timescales, between the oxidized dye and the injected electron, that remains as one of the main bottlenecks to be targeted for achieving further improved solar energy conversion efficiencies in future work.
|
|
| 4. |
- Lindh, Linnea, et al.
(författare)
-
Multifaceted Deactivation Dynamics of Fe(II) N-Heterocyclic Carbene Photosensitizers
- 2023
-
Ingår i: Journal of Physical Chemistry A. - 1089-5639. ; 127:48, s. 10210-10222
-
Tidskriftsartikel (refereegranskat)abstract
- Excited state dynamics of three iron(II) carbene complexes that serve as prototype Earth-abundant photosensitizers were investigated by ultrafast optical spectroscopy. Significant differences in the dynamics between the investigated complexes down to femtosecond time scales are used to characterize fundamental differences in the depopulation of triplet metal-to-ligand charge-transfer (3MLCT) excited states in the presence of energetically accessible triplet metal-centered (3MC) states. Novel insights into the full deactivation cascades of the investigated complexes include evidence of the need to revise the deactivation model for a prominent iron carbene prototype complex, a refined understanding of complex 3MC dynamics, and a quantitative discrimination between activated and barrierless deactivation steps along the 3MLCT → 3MC → 1GS path. Overall, the study provides an improved understanding of photophysical limitations and opportunities for the use of iron(II)-based photosensitizers in photochemical applications.
|
|
| 5. |
- Lindh, Linnea, et al.
(författare)
-
Side-group switching between metal-to-ligand charge-transfer and metal-centered excited state properties in iron(II) N-heterocyclic carbene complexes
- 2024
-
Ingår i: Coordination Chemistry Reviews. - 0010-8545. ; 506
-
Forskningsöversikt (refereegranskat)abstract
- Fe(II) N-heterocyclic carbene (NHC) complexes have emerged over the last decade as a promising class of light-harvesting complexes for a variety of photochemical applications relying on the presence of high-energy excited states of mainly charge-transfer character with excited state lifetimes of tens of picoseconds or longer. Recent spectroscopic investigations have significantly refined the understanding of some of the key prototype complexes of this kind and highlighted the subtle balance between population of triplet metal-to-ligand charge-transfer (3MLCT) and triplet metal-centered (3MC) states as a key issue to better understand and ultimately control the excited state dynamics in these complexes. To present a broader perspective on this issue, we here re-examine and discuss the excited state properties of a series of complexes with different side-groups on a common Fe NHC scaffold. Both the steady-state absorption spectrum and excited state dynamics are influenced by the side-group substitution, and the changes are rationalized based on shifting of the lowest metal-to-ligand charge-transfer (MLCT) state in energy based on the electron-withdrawing or electron-donating properties of the side-groups. Only electron-withdrawing substituents such as carboxylic acid groups ensured that the majority excited population stays in the 3MLCT state for ∼20 ps rather than rapidly converting into metal-centered (MC) states. In other complexes, the 3MLCT state survived <300 fs after which the 3MC state was populated for ∼10 ps. The transient absorption results also show that the dynamics can be switched in a simple manner by deprotonating the carboxylic acid group, which renders some of the complexes pH-sensitive. For the here discussed complexes, the results from transient absorption measurements indicate that the 3MLCT and 3MC states were close enough in energy to enable the side-group to determine the photophysics. The emerging understanding of the 3MLCT-3MC balance, as well as the nature and properties of the 3MC state in these complexes with intermediate ligand field strength is used to provide a broader fundamental perspective required to improve the ligand-design of Fe carbene complexes for issues such as to ensure a long-lived 3MLCT state.
|
|
| 6. |
- Moberg, Christina, et al.
(författare)
-
De unga gör helt rätt när de stämmer staten
- 2022
-
Ingår i: Aftonbladet. - 1103-9000.
-
Tidskriftsartikel (populärvet., debatt m.m.)abstract
- 1 620 forskare och lärare i forskarvärlden: Vi ställer oss bakom Auroras klimatkrav
|
|
| 7. |
- Nyberg, Lena, 1979, et al.
(författare)
-
A single-step competitive binding assay for mapping of single DNA molecules
- 2012
-
Ingår i: Biochemical and Biophysical Research Communications - BBRC. - : Elsevier BV. - 0006-291X .- 1090-2104. ; 417:1, s. 404-408
-
Tidskriftsartikel (refereegranskat)abstract
- Optical mapping of genomic DNA is of relevance for a plethora of applications such as scaffolding for sequencing and detection of structural variations as well as identification cif pathogens like bacteria and viruses. For future clinical applications it is desirable to have a fast and robust mapping method based on as few steps as possible. We here demonstrate a single-step method to obtain a DNA barcode that is directly visualized using nanofluidic devices and fluorescence microscopy. Using a mixture of YOYO-1, a bright DNA dye, and netropsin, a natural antibiotic with very high AT specificity, we obtain a DNA map with a fluorescence intensity profile along the DNA that reflects the underlying sequence. The netropsin binds to AT-tetrads and blocks these binding sites from YOYO-1 binding which results in lower fluorescence intensity from AT-rich regions of the DNA. We thus obtain a DNA barcode that is dark in AT-rich regions and bright in GC-rich regions with kilobasepair resolution. We demonstrate the versatility of the method by obtaining a barcode on DNA from the phage T4 that captures its circular permutation and agrees well with its known sequence.
|
|
| 8. |
|
|
| 9. |
- Alalam, Hanna, et al.
(författare)
-
A High-Throughput Method for Screening for Genes Controlling Bacterial Conjugation of Antibiotic Resistance.
- 2020
-
Ingår i: mSystems. - 2379-5077. ; 5:6
-
Tidskriftsartikel (refereegranskat)abstract
- The rapid horizontal transmission of antibiotic resistance genes on conjugative plasmids between bacterial host cells is a major cause of the accelerating antibiotic resistance crisis. There are currently no experimental platforms for fast and cost-efficient screening of genetic effects on antibiotic resistance transmission by conjugation, which prevents understanding and targeting conjugation. We introduce a novel experimental framework to screen for conjugation-based horizontal transmission of antibiotic resistance between >60,000 pairs of cell populations in parallel. Plasmid-carrying donor strains are constructed in high-throughput. We then mix the resistance plasmid-carrying donors with recipients in a design where only transconjugants can reproduce, measure growth in dense intervals, and extract transmission times as the growth lag. As proof-of-principle, we exhaustively explore chromosomal genes controlling F-plasmid donation within Escherichia coli populations, by screening the Keio deletion collection in high replication. We recover all seven known chromosomal gene mutants affecting conjugation as donors and identify many novel mutants, all of which diminish antibiotic resistance transmission. We validate nine of the novel genes' effects in liquid mating assays and complement one of the novel genes' effect on conjugation (rseA). The new framework holds great potential for exhaustive disclosing of candidate targets for helper drugs that delay resistance development in patients and societies and improve the longevity of current and future antibiotics. Further, the platform can easily be adapted to explore interspecies conjugation, plasmid-borne factors, and experimental evolution and be used for rapid construction of strains.IMPORTANCE The rapid transmission of antibiotic resistance genes on conjugative plasmids between bacterial host cells is a major cause of the accelerating antibiotic resistance crisis. There are currently no experimental platforms for fast and cost-efficient screening of genetic effects on antibiotic resistance transmission by conjugation, which prevents understanding and targeting conjugation. We introduce a novel experimental framework to screen for conjugation-based horizontal transmission of antibiotic resistance between >60,000 pairs of cell populations in parallel. As proof-of-principle, we exhaustively explore chromosomal genes controlling F-plasmid donation within E. coli populations. We recover all previously known and many novel chromosomal gene mutants that affect conjugation efficiency. The new framework holds great potential for rapid screening of compounds that decrease transmission. Further, the platform can easily be adapted to explore interspecies conjugation, plasmid-borne factors, and experimental evolution and be used for rapid construction of strains.
|
|
| 10. |
- Alalam, Hanna, et al.
(författare)
-
Conjugation factors controlling F-plasmid antibiotic resistance transmission
- 2018
-
Ingår i: BioRxiv. - : Cold Spring Harbor Laboratory.
-
Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)abstract
- The rapid horizontal transmission of many antibiotic resistance genes between bacterial host cells on conjugative plasmids is a major cause of the accelerating antibiotic resistance crisis. Preventing understanding and targeting conjugation, there currently are no experimental platforms for fast and cost-efficient screening of genetic effects on antibiotic resistance transmission by conjugation. We introduce a novel experimental framework to screen for conjugation based horizontal transmission of antibiotic resistance between >60,000 pairs of cell populations in parallel. Plasmid-carrying donor strains are constructed in high throughput. We then mix the resistance plasmid carrying donors with recipients in a design where only transconjugants can reproduce, measure growth in dense intervals and extract transmission times as the growth lag. As proof-of-principle, we exhaustively explored chromosomal genes controlling F plasmid donation within E. coli populations, by screening the Keio deletion collection at high replication. We recover all six known chromosomal gene mutants affecting conjugation and identify >50 novel factors, all of which diminish antibiotic resistance transmission. We verify 10 of the novel genes' effects in a liquid mating assay. The new framework holds great potential for exhaustive disclosing of candidate targets for helper drugs that delay resistance development in patients and societies and improves the longevity of current and future antibiotics.
|
|
| 11. |
- Alfonzo, Emilia, et al.
(författare)
-
No survival difference between robotic and open radical hysterectomy for women with early-stage cervical cancer: results from a nationwide population-based cohort study
- 2019
-
Ingår i: European Journal of Cancer. - : Elsevier BV. - 0959-8049 .- 1879-0852. ; 116, s. 169-177
-
Tidskriftsartikel (refereegranskat)abstract
- Purpose: The aim of the study was to compare overall survival (OS) and disease-free survival (DFS) after open and robotic radical hysterectomy for early-stage cervical cancer. Patients and methods: This was a nationwide population-based cohort study on all women with cervical cancer stage IA1-IB of squamous, adenocarcinoma or adenosquamous histological subtypes, from January 2011 to December 2017, for whom radical hysterectomy was performed. The Swedish Quality Register of Gynaecologic Cancer was used for identification. To ensure quality and conformity of data and to disclose patients not yet registered, hospital registries were reviewed and validated. Cox and propensity score regression analysis and univariable and multivariable regression analysis were performed in regard to OS and DFS. Results: There were 864 women (236 open and 628 robotic) included in the study. The 5-year OS was 92% and 94% and DFS was 84% and 88% for the open and robotic cohorts, respectively. The recurrence pattern was similar in both groups. Using propensity score analysis and matched cohorts of 232 women in each surgical group, no significant differences were seen in survival: 5-year OS of 92% in both groups (hazard ratio [HR], 1.00; 95% confidence interval [CI], 0.50–2.01) and DFS of 85% vs 84% in the open and robotic cohort, respectively (HR, 1.08; 95% CI, 0.66–1.78). In univariable and multivariable analysis with OS as the end-point, no significant factors were found, and in regard to DFS, tumour size (p < 0.001) and grade 3 (p = 0.02) were found as independent significant risk factors. Conclusion: In a complete nationwide population-based cohort, where radical hysterectomy for early-stage cervical cancer is highly centralised, neither long-term survival nor pattern of recurrence differed significantly between open and robotic surgery. © 2019 The Authors
|
|
| 12. |
- Andersson, Olov, 1979-, et al.
(författare)
-
WARA-PS : a research arena for public safety demonstrations and autonomous collaborative rescue robotics experimentation
- 2021
-
Ingår i: Autonomous Intelligent Systems. - : Springer Nature. - 2730-616X. ; 1:1
-
Tidskriftsartikel (refereegranskat)abstract
- A research arena (WARA-PS) for sensing, data fusion, user interaction, planning and control of collaborative autonomous aerial and surface vehicles in public safety applications is presented. The objective is to demonstrate scientific discoveries and to generate new directions for future research on autonomous systems for societal challenges. The enabler is a computational infrastructure with a core system architecture for industrial and academic collaboration. This includes a control and command system together with a framework for planning and executing tasks for unmanned surface vehicles and aerial vehicles. The motivating application for the demonstration is marine search and rescue operations. A state-of-art delegation framework for the mission planning together with three specific applications is also presented. The first one concerns model predictive control for cooperative rendezvous of autonomous unmanned aerial and surface vehicles. The second project is about learning to make safe real-time decisions under uncertainty for autonomous vehicles, and the third one is on robust terrain-aided navigation through sensor fusion and virtual reality tele-operation to support a GPS-free positioning system in marine environments. The research results have been experimentally evaluated and demonstrated to industry and public sector audiences at a marine test facility. It would be most difficult to do experiments on this large scale without the WARA-PS research arena. Furthermore, these demonstrator activities have resulted in effective research dissemination with high public visibility, business impact and new research collaborations between academia and industry.
|
|
| 13. |
- Anjemark, Linnea, et al.
(författare)
-
Car accidents in drivers with Parkinson's disease or multiple sclerosis: A Swedish nationwide study
- 2023
-
Ingår i: European Journal of Neurology. - : Wiley. - 1351-5101 .- 1468-1331. ; 30:6, s. 1631-1638
-
Tidskriftsartikel (refereegranskat)abstract
- Background and purpose: Parkinson's disease (PD) and multiple sclerosis (MS) can impair driving. However, we lack evidence on car accidents associated with these diseases. The aims of this study were to examine what types of car accident were associated with drivers with PD and MS, compared to individuals with ulcerative colitis (UC; the comparison group), and to evaluate the occurrence of car accidents in relation to years since diagnosis.Methods: This retrospective nationwide, registry-based study included drivers involved in car accidents between 2010 and 2019, based on the Swedish Traffic Accident Data Acquisition database. Data on pre-existing diagnoses were retrieved retrospectively from the National Patient Registry. Data analyses included group comparisons, time-to-event analysis, and binary logistic regression.Results: In total, 1491 drivers, including 199 with PD, 385 with MS, and 907 with UC, were registered to have been involved in a car accident. The mean time from diagnosis to the car accident was 5.6 years for PD, 8.0 years for MS, and 9.4 years for UC. Time to car accident since diagnosis differed significantly (p < 0.001) among groups (adjusted for age). Drivers with PD had more than twice the odds of a single-car accident than drivers with MS or UC, but no differences were observed between MS and UC.Conclusions: Drivers with PD were older and experienced the car accident within a shorter timeframe after disease diagnosis. Although several factors may cause a car accident, fitness to drive could be more thoroughly evaluated for patients with PD by physicians, even early after the diagnosis.
|
|
| 14. |
- Bereza-Jarocinski, Robert, et al.
(författare)
-
Distributed Model Predictive Control for Cooperative Landing
- 2020
-
Ingår i: Proceedings 21st IFAC World Congress on Automatic Control - Meeting Societal Challenges. - : Elsevier BV. ; , s. 15180-15185
-
Konferensbidrag (refereegranskat)abstract
- We design, implement and test two control algorithms for autonomously landing a drone on an autonomous boat. The first algorithm uses distributed model predictive control (DMPC), while the second combines a cascade controller with DMPC. The algorithms are implemented on a real drone, while the boat's motion is simulated, and their performance is compared to a centralized model predictive controller. Field experiments are performed, where all algorithms show an ability to land while avoiding violation of the safety constraints. The two distributed algorithms further show the ability to use longer prediction horizons than the centralized model predictive controller, especially in the cascade case, and also demonstrate improved robustness towards breaks in communication.
|
|
| 15. |
- Bergström, Rasmus, et al.
(författare)
-
SCATTERING OF RADAR WAVES ON AEROSOLS IN PLASMAS
- 2015
-
Ingår i: EUROPEAN ROCKET AND BALLOON. - 9789292212940 ; , s. 87-94
-
Konferensbidrag (refereegranskat)abstract
- To study the physical mechanisms of phenomena such as polar mesospheric summer echoes, the SCRAP (Scattering of Radar waves on Aerosols in Plasmas) experiment aimed to validate theories on density fluctuations in dusty plasmas. The SCRAP team developed two identical free falling units (FFUs) designed to create a cloud of copper particles once they eject from the REXUS17 sounding rocket 124 seconds after launch. By using the EISCAT incoherent scatter radar system to observe the cloud, the SCRAP experiment proposed to relate theoretical predictions to a controlled object. The SCRAP experiment was launched from ESRANGE on March the 17th 2015. The FFUs GPS signal was lost during launch and the units were therefore not found. Moreover, no backscattering from the copper cloud was observed by the radar.
|
|
| 16. |
- Björn, Linnea, 1994, et al.
(författare)
-
Process-Induced Structures of Injection-Molded High-Density Polyethylene─Combining X-ray Scattering and Finite Element Modeling
- 2024
-
Ingår i: ACS Applied Polymer Materials. - 2637-6105. ; 6:8, s. 4852-4864
-
Tidskriftsartikel (refereegranskat)abstract
- The success of plastics heavily relies on fast melt processing methods used for large-scale industrial manufacturing, including injection molding. The hierarchical structure of the solid polymer depends on material selection combined with processing conditions, making mechanical properties of the injection molded part difficult to predict. Here we show how scanning small- and wide-angle X-ray scattering, birefringence microscopy, and polarized light optical microscopy can be combined with injection molding simulations to shed light on the correlation between the polymer morphology of high-density polyethylene and processing conditions. The scattering data revealed that the complex layered structure highly depends on the pressure during the holding phase of injection molding. Furthermore, we identified specific work of flow as a main parameter to capture the changes in morphology induced by varying the process settings. Overall, a good agreement was found between experimental data and the computational simulations, suggesting that computational simulations can be further used to predict the multiphase morphology of injection molded parts.
|
|
| 17. |
- Björn, Linnea, 1994, et al.
(författare)
-
Scanning Small-Angle X-ray Scattering of Injection-Molded Polymers: Anisotropic Structure and Mechanical Properties of Low-Density Polyethylene
- 2023
-
Ingår i: ACS Applied Polymer Materials. - 2637-6105. ; 5:8, s. 6429-6440
-
Tidskriftsartikel (refereegranskat)abstract
- Injection molding is known to create a layered anisotropicmorphologyacross the sample thickness due to varying shear and cooling ratesduring the manufacturing process. In this study, scanning small-angleX-ray scattering was used to visualize and quantify the distributionof hierarchical structures present in injection-molded parts of low-densitypolyethylene (LDPE) with varying viscosities. By combining scatteringdata with results from injection molding simulations and tensile testing,we find that oriented shish-kebab structures, as well as elongatedspherulite structures consisting of semicrystalline ellipsoids, contributeto high ultimate tensile strength along the flow direction. Furthermore,we show that a higher degree of orientation is found close to theinjection gate and in LDPE with higher viscosity, consequently fromelevated shear and cooling rates present during the injection moldingprocess.
|
|
| 18. |
- Braam, Svenja, 1989, et al.
(författare)
-
Exploring carbon source related localization and phosphorylation in the Snf1/Mig1 network using population and single cell-based approaches
- 2024
-
Ingår i: Microbial Cell. - 2311-2638. ; 11:1, s. 143-154
-
Tidskriftsartikel (refereegranskat)abstract
- The AMPK/SNF1 pathway governs energy balance in eukaryotic cells, notably influencing glucose de-repression. In S. cerevisiae, Snf1 is phosphorylated and hence activated upon glucose depletion. This activation is required but is not sufficient for mediating glucose de-repression, indicating further glucose-dependent regulation mechanisms. Employing fluorescence recovery after photobleaching (FRAP) in conjunction with non-linear mixed effects modelling, we explore the spatial dynamics of Snf1 as well as the relationship between Snf1 phosphorylation and its target Mig1 controlled by hexose sugars. Our results suggest that inactivation of Snf1 modulates Mig1 localization and that the kinetic of Snf1 localization to the nucleus is modulated by the presence of non-fermentable carbon sources. Our data offer insight into the true complexity of regulation of this central signaling pathway in orchestrating cellular responses to fluctuating environmental cues. These insights not only expand our understanding of glucose homeostasis but also pave the way for further studies evaluating the importance of Snf1 localization in relation to its phosphorylation state and regulation of downstream targets.
|
|
| 19. |
|
|
| 20. |
- Chábera, Pavel, et al.
(författare)
-
Band-selective dynamics in charge-transfer excited iron carbene complexes
- 2019
-
Ingår i: Faraday Discussions. - : Royal Society of Chemistry (RSC). - 1359-6640 .- 1364-5498. ; 216:2019, s. 191-210
-
Tidskriftsartikel (refereegranskat)abstract
- Ultrafast dynamics of photoinduced charge transfer processes in light-harvesting systems based on Earth-abundant transition metal complexes are of current interest for the development of molecular devices for solar energy conversion applications. A combination of ultrafast spectroscopy and first principles quantum chemical calculations of a recently synthesized iron carbene complex is used to elucidate the ultrafast excited state evolution processes in these systems with particular emphasis on investigating the underlying reasons why these complexes show promise in terms of significantly extended lifetimes of charge transfer excited states. Together, our results challenge the traditional excited state landscape for iron-based light harvesting transition metal complexes through radically different ground and excited state properties in alternative oxidation states. This includes intriguing indications of rich band-selective excited state dynamics on ultrafast timescales that are interpreted in terms of excitation energy dependence for excitations into a manifold of charge-transfer states. Some implications of the observed excited state properties and photoinduced dynamics for the utilization of iron carbene complexes for solar energy conversion applications are finally discussed.
|
|
| 21. |
- Chábera, Pavel, et al.
(författare)
-
Photofunctionality of iron(III) N-heterocyclic carbenes and related d5 transition metal complexes
- 2021
-
Ingår i: Coordination Chemistry Reviews. - : Elsevier BV. - 0010-8545. ; 426
-
Forskningsöversikt (refereegranskat)abstract
- Despite a few reports of photoluminescent and strongly photo-oxidizing transition metal complexes with a d5 electronic configuration, the photophysics and photochemistry of this class of transition metal complexes have largely remained unexplored. Recent investigations of earth-abundant iron(III) N-heterocyclic carbene (NHC) complexes have demonstrated promising photophysical and photochemical properties associated with low-spin (doublet) ligand-to-metal charge transfer (2LMCT) excitations, including nanosecond photoluminescence (PL) and capabilities to drive both photo-oxidation and photo-reduction reactions. These encouraging results are at first sight surprising in light of the general scarcity of known photofunctional complexes of any transition metal complexes with a d5 electronic configuration, including 1st, 2nd and 3rd row transition metal complexes of Mn(II), Tc(II), Re(II), Fe(III), Ru(III) and Os(III). Here, we review the photophysical and photochemical properties of the new Fe(III) NHC complexes together with related d5 transition metal complexes as a basis for a broader understanding of the unorthodox photophysical and photochemical properties associated with this open-shell electronic configuration. This includes considerations of the role of charge and spin effects on the ground state electronic structure, as well as discussions of charge transfer (CT) and metal centered (MC) excited state properties. The special properties of 2LMCT excited states are emphasized as a key feature to understand the photophysics of many photofunctional d5 transition metal complexes. Further aspects of excited state dynamics with d5 light-harvesting complexes, including both intra- and inter-molecular charge transfer processes, are also discussed. Finally, some fundamental challenges and emerging opportunities for further development of photofunctional Fe(III) NHC and related LMCT/d5 complexes for light-harvesting, light-emitting, and photo(electro)chemical applications are outlined. This includes some general considerations of how the specific photochemical properties of the LMCT/d5 complexes provides an exciting opportunity to develop a unique niche within the diversity of photofunctional molecular systems alongside other types of organic and inorganic chromophores commonly used in the field of molecular photochemistry.
|
|
| 22. |
- Dumitrescu, Delia, et al.
(författare)
-
Smart Textiles as raw materials for design
- 2014
-
Ingår i: Shapeshifting. - Auckland, New Zealand : Textile and Design Laboratory and Colab, Auckland University of Technology. - 9781927184271
-
Konferensbidrag (refereegranskat)abstract
- Materials fabricate the designed artefact, but they can also play an important role in the design process; as a medium or method used to develop the design. Textiles can, with their soft and flexible properties, be easily transformed and altered in numerous ways; for example, by cutting, folding orprinting on the material. This transformative character makes textiles interesting sketching media for surface explorations when designing artefacts. The development of transformable materials; for example, fusible yarns and colour changing pigments, have expanded these inherent transformative qualities of textiles and have opened up the design field of smart textiles. Accordingly, this new material context has created a new area for textile designers to explore, where it is possible to enhance and play with the alterable character of their textiles, and control their transformation through physical manipulation and programming. However, these expanded transformative properties also open up a new task for textile designers; to design "smart textiles as raw materials for design". By this term we mean, textiles that are not finished in their design but that can be developed and enhanced when they take part in a product or space design process. In this article, we explore and start to define what smart textiles as raw materials for design can be, and look at how these materials can come into and add something to another design process. The foundation for this exploration is a number of textile examples from the “Smart Textiles sample collection” and our experiences when developing and designing with them. (The Smart Textiles sample collection is a range of textiles that is designed and produced by the Smart Textile Design Lab, to give students, designers and researchers direct access to different types of smart textiles). The possibilities and limitations of smart textiles as raw materials for design are explored by looking at the textile examples from two perspectives: firstly, by looking at the considerations that come with designing this type of textile design, and secondly by looking at what these transformative textiles can bring to another design process. Each example is analyzed and classified according to what transformable design variables for structure and surface change can be embedded in the textile design, and what design variables this subsequently creates for a design process that uses these materials i.e., describing what type of transformation different examples of smart textiles introduce to the design process/design space; whether the change is reversible or irreversible, and whether the change occurs through physical or through digital manipulation of the material. This article ends with a discussion of how smart textiles in the form of raw materials for design could influence how we design textiles and how we design with textiles. Can transformative materials enrich material explorations in a design process? Can further development and alteration of the material design be introduced or defined by the textile designer? Could smart textiles as raw materials for design open up a stronger connection between the design of textiles and the design of the product or spaces where they will be used?
|
|
| 23. |
- Ekblom, Robert, Docent, 1976-, et al.
(författare)
-
Genome sequencing and conservation genomics in the Scandinavian wolverine population
- 2018
-
Ingår i: Conservation Biology. - : WILEY. - 0888-8892 .- 1523-1739. ; 32:6, s. 1301-1312
-
Tidskriftsartikel (refereegranskat)abstract
- Genetic approaches have proved valuable to the study and conservation of endangered populations, especially for monitoring programs, and there is potential for further developments in this direction by extending analyses to the genomic level. We assembled the genome of the wolverine (Gulo gulo), a mustelid that in Scandinavia has recently recovered from a significant population decline, and obtained a 2.42 Gb draft sequence representing >85% of the genome and including >21,000 protein-coding genes. We then performed whole-genome resequencing of 10 Scandinavian wolverines for population genomic and demographic analyses. Genetic diversity was among the lowest detected in a red-listed population (mean genome-wide nucleotide diversity of 0.05%). Results of the demographic analyses indicated a long-term decline of the effective population size (N-e) from 10,000 well before the last glaciation to N-e appeared even lower. The genome-wide F-IS level was 0.089 (possibly signaling inbreeding), but this effect was not observed when analyzing a set of highly variable SNP markers, illustrating that such markers can give a biased picture of the overall character of genetic diversity. We found significant population structure, which has implications for population connectivity and conservation. We used an integrated microfluidic circuit chip technology to develop an SNP-array consisting of 96 highly informative markers that, together with a multiplex pre-amplification step, was successfully applied to low-quality DNA from scat samples. Our findings will inform management, conservation, and genetic monitoring of wolverines and serve as a genomic roadmap that can be applied to other endangered species. The approach used here can be generally utilized in other systems, but we acknowledge the trade-off between investing in genomic resources and direct conservation actions.
|
|
| 24. |
- Ekdahl, Linnea, et al.
(författare)
-
A combination of second trimester oral metronidazole and no sexual intercourse during second and third trimester may reduce late miscarriage and premature delivery after fertility sparing radical trachelectomy
- 2021
-
Ingår i: European Journal of Obstetrics and Gynecology and Reproductive Biology. - : Elsevier BV. - 0301-2115 .- 1872-7654. ; 265, s. 90-95
-
Tidskriftsartikel (refereegranskat)abstract
- Objectives: Women with a previous trachelectomy have an increased risk of premature delivery and second trimester miscarriage. In this study we aim to evaluate factors and regimes possibly affecting the risk for prematurity following fertility sparing robotic radical trachelectomy (RRT) in cervical cancer. Methods: A retrospective study of the reproductive outcome following RRT with a cervical cerclage performed at one of four academic centers between 2007 and 2019. Factors possibly related to premature delivery, such as postoperative non-pregnant cervical length, previous vaginal deliveries, preservation of the uterine arteries, and the use of a second trimester oral metronidazole/no sexual intercourse regime, were assessed. Results: 109 women remained for analyses after excluding recurrences before pregnancy (n = 8), secondary hysterectomy (n = 2), and women with less than six months follow up (n = 10). 74 pregnancies occurred in 52/71 women attempting to conceive, 56 of which developed past the first trimester. Two of 22 women (9%) who were prescribed an oral metronidazole regime (400 mg × 2 from gestational week 15 + 0 to 21 + 6 and abstaining from sexual intercourse for the duration of the pregnancy) had a premature delivery, compared with 13/31 (42%) where the regime was not applied (p = 0.009). The association remained after regression analyses including possible contributing factors as of above, none of which associated with prematurity at regression analyses (p = 0.001). Conclusions: The observed four-fold reduction in premature delivery indicates that an oral metronidazole/no sexual intercourse regime may reduce second trimester miscarriage and premature deliveries following an RRT. No association was observed for other investigated factors.
|
|
| 25. |
- Ekdahl, Linnea, et al.
(författare)
-
Increased Institutional Surgical Experience in Robot-Assisted Radical Hysterectomy for Early Stage Cervical Cancer Reduces Recurrence Rate: Results from a Nationwide Study
- 2020
-
Ingår i: JOURNAL OF CLINICAL MEDICINE. - : MDPI AG. - 2077-0383. ; 9:11
-
Tidskriftsartikel (refereegranskat)abstract
- The aim of this study was to evaluate the impact of institutional surgical experience on recurrence following robotic radical hysterectomy (RRH) for early stage cervical cancer. All women in Sweden who underwent an RRH for stage IA2-IB1 cervical cancer at tertiary referral centers from its implementation in December 2005 until June 2017 were identified using a Swedish nationwide register and local hospital registers. Registry data were controlled by a chart review of all women. Recurrence rates and patterns of recurrence were compared between early and late (<= 50 vs. >50 procedures) institutional series. Six hundred and thirty-five women were included. Regression analysis identified a lower risk of recurrence with increased experience but without a clear cut off level. Among the 489 women who did not receive adjuvant radio chemotherapy (RC-T), the rate of recurrence was 3.6% in the experienced cohort (>50 procedures) compared to 9.3% in the introductory cohort (p < 0.05). This was also seen in tumors < 2 cm regardless of RC-T (p < 0.05), whereas no difference in recurrence was seen when analyzing all women receiving RC-T. In conclusion, the rate of recurrence following RRH for early stage cervical cancer decreased with increased institutional surgical experience, in tumors < 2 cm and in women who did not receive adjuvant RC-T.
|
|
| 26. |
- Ekdahl, Linnea, et al.
(författare)
-
Long term oncologic and reproductive outcomes after robot-assisted radical trachelectomy for early-stage cervical cancer. An international multicenter study
- 2022
-
Ingår i: Gynecologic Oncology. - : Elsevier BV. - 0090-8258 .- 1095-6859. ; 164:3, s. 529-534
-
Tidskriftsartikel (refereegranskat)abstract
- Objectives: Long term outcomes following fertility sparing robot-assisted radical trachelectomy (RRT). Methods: A retrospective study of consecutive women selected for RRT between 2007 and 2019 at five referral centres. Generally used selection criteria for fertility-sparing surgery were applied. Oncologic, reproductive and long-term clinical data were analysed. Results: Of the 166 included women, 149 completed a RRT. Median tumor size was 9 mm (range 3-20 mm), 111 women (75%) had FIGO 2009 stage IB1 cancer and 4.8% were node positive. At a median follow up of 58 months, 12 of all women (7.2%) and 9 of 149 women (6%) who underwent completed RRT with fertility preservation had recurred and two had died. 70 of 88 women (80%) who attempted to conceive succeeded, resulting in 81 pregnancies that progressed beyond the first trimester and 76 live births of which 54 (70%) were delivered at term and 65 (86%) delivered after gestational week 32. A short postoperative cervical length was associated with impaired fertility. A late secondary hysterectomy was necessary in four women due to persistent bleeding (n = 2), hematometra due to a cervical stenosis (n = 1) and recurrent dysplasia (n = 1). Conclusion: In this long-term follow-up of RRT the recurrence rate is comparable to larger individual studies of minimally invasive or vaginal radical trachelectomy with similar risk profile and follow up. The high pregnancy rate and low rate of premature delivery before 32 weeks GA may promote the use of robot-assisted approach.
|
|
| 27. |
- Ekdahl, Linnea, et al.
(författare)
-
Quality of life and long-term clinical outcome following robot-assisted radical trachelectomy
- 2021
-
Ingår i: European Journal of Obstetrics and Gynecology and Reproductive Biology. - : Elsevier BV. - 0301-2115. ; 267, s. 234-240
-
Tidskriftsartikel (refereegranskat)abstract
- Quality of Life and long-term clinical outcome following robot-assisted radical trachelectomy. Objectives: To evaluate quality of life (QoL) and long-term clinical outcome following robot-assisted radical trachelectomy (RRT). Study Design: Prospectively retrieved clinical data were rereviewed on all women planned for a fertility sparing RRT for early stage cervical cancer at Skåne University Hospital, Sweden between 2007 and 2020. QoL was assessed using the validated questionnaires EORTC QLQ-C30, QLQ-CX24 and the Swedish LYMQOL. Results: Data was analyzed from 49 women, 42 with a finalised RRT and seven with an aborted RRT due to nodal metastases (n = 3) or insufficient margins (n = 4). At a median follow-up time of 54 months one recurrence (2%) occurred (aborted RRT). According to QLQ-C30 the median global health status score was 75. The disease specific QLQ-C24 showed an impact on symptoms related to sexual function where sexual/vaginal functioning had a median score of 25 and 48% of patients reported worry that sex would cause physical pain. Despite this the functional items sexual activity and sexual enjoyment both had a median score of 66.7. Lymphoedema was reported in 45%, where 9% reported severe symptom with an impact on their QoL. No intraoperative complications and no postoperative complications ≥ Clavien Dindo grade III were observed. Twenty-two of 28 (79%) women who attempted to conceive were successful. A metronidazole/no intercourse regimen was applied between GW 15 + 0–21 + 6 in 26 of 28 pregnancies beyond first trimester resulting in a 92% term (≥GW 36 + 0) delivery rate. Conclusions: Although robot-assisted radical trachelectomy in this cohort was associated with a low recurrence rate, a high fertility rate and an exceptionally high term delivery rate, women's quality of life was affected postoperatively, particularly with regards to their sexual well-being and lymphatic side-effects.
|
|
| 28. |
- Ekman, Linnéa, et al.
(författare)
-
Examining practice effects in repeated measurements of vibration perception thresholds on finger pulps of healthy individuals – Is it possible to improve your results over a clinically relevant test interval?
- 2019
-
Ingår i: PLoS ONE. - : Public Library of Science (PLoS). - 1932-6203. ; 14:12
-
Tidskriftsartikel (refereegranskat)abstract
- Aims To investigate practice effects in a test-retest situation, where vibration perception thresholds (VPT) were measured in healthy subjects using a multi-frequency test method. Methods In eight consecutive tests, VPTs were tested in the pulps of the index and little fingers at seven frequencies (8, 16, 32, 64, 125, 250 and 500 Hz). Subjects were twenty healthy adults aged 26 to 65 years (mean 46.0 ± 11.1 years; 10 male and 10 female). The subjects were examined at six tests with intervals of one month (mean 33 ± 6; time 0 to month 5) and at two additional tests with prolonged intervals (month 12 and 18). Linear mixed model analysis was performed to investigate differences over the subsequent test occasions. To examine where potential practice effects occurred, a pairwise comparison with Bonferroni correction was made. Results Small decreases in VPTs were found in 8 out of the 14 frequencies (index finger: 8, 16, 32, 250 and 500 Hz; little finger: 16, 250 and 500 Hz) within the test period from time 0 to month 5. In tests at 12 and 18 months, VPTs were increased compared to month 5, but lowered in comparison with time 0. Hence, minor significant decreases were found in three frequencies for the index finger (125, 250 and 500 Hz) and one frequency for the little finger (250 Hz) when examining VPTs with prolonged time intervals. Conclusions When evaluating vibration perception thresholds in a clinically relevant time period of once or twice a year, no consideration of practice effects is necessary when interpreting the results.
|
|
| 29. |
- Gustavsson Binder, Tobias, et al.
(författare)
-
Hydrogen from biogas as fuel for buses in cold climate - Analysing the feasibility to produce hydrogen from local biogas and use in city buses in Luleå
- 2024
-
Rapport (övrigt vetenskapligt/konstnärligt)abstract
- In this study, we demonstrate that in certain cases, it can be advantageous to produce hydrogen from biogas and to use it in heavy-duty vehicles such as buses. In Luleå, it may be feasible to use hydrogen from biogas in city buses because there is a need for heating where waste heat from the fuel cell can be utilized. However, it is uncertain whether the waste heat is sufficient or if a separate auxiliary heater driven by diesel or HVO is needed. If such a heater is required, the conclusion is that hydrogen from biogas is suitable for other segments of heavy transportation, where battery electrification is not as suitable. Overall, our study shows that hydrogen from biogas may be interesting as a transitional fuel to increase the availability of environmentally friendly hydrogen until electrolyzer capacity is sufficiently expanded.At the same time, our mapping of the policy landscape concerning hydrogen and zero-emission buses shows that biohydrogen is disadvantaged in the EU's regulations on renewable hydrogen. This means that member states are restricted from providing support for investments to produce and distribute hydrogen from biogas and other biogenic feedstocks. The reason is that renewable hydrogen, according to EU terminology, is defined in the so-called delegated act on renewable fuels of non-biological origin (RFNBO). It is established that renewable hydrogen should be based on non-biological feedstocks (i.e., from electrolysis) and must meet a number of criteria.The results are interesting in the context of urban bus traffic rapidly moving towards zero-emission operation. In Sweden and many other countries, battery buses have become a common and obvious feature on city streets. But just like for other segments of heavy-duty vehicles, another technology to achieve zero-emission operation has also received increased attention, namely hydrogen and fuel cell buses. In Sweden, only a few fuel cell buses have been used - and moreover, only on a trial basis - but in several European cities, they have already begun to be used on a significant scale. An advantage of fuel cell operation with hydrogen from biogas is that it allows for the continued utilization of the biogas already produced and purchased for existing city bus traffic.System study consisting of two partsWe arrived at the result by investigating the suitability of both producing hydrogen from biogas at the existing sewage treatment plant in Luleå and the feasibility for LLT to use fuel cell buses in its city bus traffic. The study has considered both costs associated with each part and climate impact from a life cycle perspective for fuel production and bus operation.
|
|
| 30. |
- Gustavsson, Emil, 1987, et al.
(författare)
-
Modulation of Structural Heterogeneity Controls Phytochrome Photoswitching
- 2020
-
Ingår i: Biophysical Journal. - : Elsevier BV. - 0006-3495 .- 1542-0086. ; 118:2, s. 415-421
-
Tidskriftsartikel (refereegranskat)abstract
- Phytochromes sense red/far-red light and control many biological processes in plants, fungi, and bacteria. Although the crystal structures of dark- and light-adapted states have been determined, the molecular mechanisms underlying photoactivation remain elusive. Here, we demonstrate that the conserved tongue region of the PHY domain of a 57-kDa photosensory module of Deinococcus radiodurans phytochrome changes from a structurally heterogeneous dark state to an ordered, light-activated state. The results were obtained in solution by utilizing a laser-triggered activation approach detected on the atomic level with high-resolution protein NMR spectroscopy. The data suggest that photosignaling of phytochromes relies on careful modulation of structural heterogeneity of the PHY tongue.
|
|
| 31. |
- Isaksson, Linnéa, et al.
(författare)
-
Signaling Mechanism of Phytochromes in Solution.
- 2021
-
Ingår i: Structure. - : Elsevier BV. - 1878-4186 .- 0969-2126. ; 29:2, s. 151-160
-
Tidskriftsartikel (refereegranskat)abstract
- Phytochrome proteins guide the red/far-red photoresponse of plants, fungi, and bacteria. Crystal structures suggest that the mechanism of signal transduction from the chromophore to the output domains involves refolding of the so-called PHY tongue. It is currently not clear how the two other notable structural features of the phytochrome superfamily, the so-called helical spine and a knot in the peptide chain, are involved in photoconversion. Here, we present solution NMR data of the complete photosensory core module from Deinococcus radiodurans. Photoswitching between the resting and the active states induces changes in amide chemical shifts, residual dipolar couplings, and relaxation dynamics. All observables indicate a photoinduced structural change in the knot region and lower part of the helical spine. This implies that a conformational signal is transduced from the chromophore to the helical spine through the PAS and GAF domains. The discovered pathway underpins functional studies of plant phytochromes and may explain photosensing by phytochromes under biological conditions.
|
|
| 32. |
- Kaufhold, Simon, et al.
(författare)
-
Microsecond Photoluminescence and Photoreactivity of a Metal-Centered Excited State in a Hexacarbene-Co(III) Complex
- 2021
-
Ingår i: Journal of the American Chemical Society. - : American Chemical Society (ACS). - 0002-7863 .- 1520-5126. ; , s. 1307-1312
-
Tidskriftsartikel (refereegranskat)abstract
- The photofunctionality of the cobalt-hexacarbene complex [Co(III)(PhB(MeIm)3)2]+ (PhB(MeIm)3 = tris(3-methylimidazolin-2-ylidene)(phenyl)borate) has been investigated by time-resolved optical spectroscopy. The complex displays a weak (φ ∼10-4) but remarkably long-lived (τ ∼1 μs) orange photoluminescence at 690 nm in solution at room temperature following excitation with wavelengths shorter than 350 nm. The strongly red-shifted emission is assigned from the spectroscopic evidence and quantum chemical calculations as a rare case of luminescence from a metal-centered state in a 3d6 complex. Singlet oxygen quenching supports the assignment of the emitting state as a triplet metal-centered state and underlines its capability of driving excitation energy transfer processes.
|
|
| 33. |
- Kjær, Kasper Skov, et al.
(författare)
-
Luminescence and reactivity of a charge-transfer excited iron complex with nanosecond lifetime
- 2019
-
Ingår i: Science. - : American Association for the Advancement of Science (AAAS). - 0036-8075 .- 1095-9203. ; 363:6424, s. 249-253
-
Tidskriftsartikel (refereegranskat)abstract
- Iron’s abundance and rich coordination chemistry are potentially appealing features for photochemical applications. However, the photoexcitable charge-transfer (CT) states of most Fe complexes are limited by picosecond or sub-picosecond deactivation through low-lying metal centered (MC) states, resulting in inefficient electron transfer reactivity and complete lack of photoluminescence. Here we show that octahedral coordination of Fe(III) by two mono-anionic facial tris-carbene ligands can suppress such deactivation dramatically. The resulting complex [Fe(phtmeimb)2]+, where phtmeimb is [phenyl(tris(3-methylimidazol-1-ylidene))borate]-, exhibits strong, visible, room temperature photoluminescence with a 2.0 ns lifetime and 2% quantum yield via spin-allowed transition from a ligand-to-metal charge-transfer (2 LMCT) state to the ground state (2 GS). Reductive and oxidative electron transfer reactions were observed for the2 LMCT state of [Fe(phtmeimb)2]+ in bimolecular quenching studies with methylviologen and diphenylamine.
|
|
| 34. |
- La Fleur, Linnea, et al.
(författare)
-
Expression of scavenger receptor MARCO defines a targetable tumor-associated macrophage subset in non-small cell lung cancer
- 2018
-
Ingår i: International Journal of Cancer. - : WILEY. - 0020-7136 .- 1097-0215. ; 143:7, s. 1741-1752
-
Tidskriftsartikel (refereegranskat)abstract
- Tumor-associated macrophages (TAMs) are attractive targets for immunotherapy. Recently, studies in animal models showed that treatment with an anti-TAM antibody directed against the scavenger receptor MARCO resulted in suppression of tumor growth and metastatic dissemination. Here we investigated the expression of MARCO in relation to other macrophage markers and immune pathways in a non-small cell lung cancer (NSCLC) cohort (n=352). MARCO, CD68, CD163, MSR1 and programmed death ligand-1 (PD-L1) were analyzed by immunohistochemistry and immunofluorescence, and associations to other immune cells and regulatory pathways were studied in a subset of cases (n=199) with available RNA-seq data. We observed a large variation in macrophage density between cases and a strong correlation between CD68 and CD163, suggesting that the majority of TAMs present in NSCLC exhibit a protumor phenotype. Correlation to clinical data only showed a weak trend toward worse survival for patients with high macrophage infiltration. Interestingly, MARCO was expressed on a distinct subpopulation of TAMs, which tended to aggregate in close proximity to tumor cell nests. On the transcriptomic level, we found a positive association between MARCO gene expression and general immune response pathways including strong links to immunosuppressive TAMs, T-cell infiltration and immune checkpoint molecules. Indeed, a higher macrophage infiltration was seen in tumors expressing PD-L1, and macrophages residing within tumor cell nests co-expressed MARCO and PD-L1. Thus, MARCO is a potential new immune target for anti-TAM treatment in a subset of NSCLC patients, possibly in combination with available immune checkpoint inhibitors.
|
|
| 35. |
- Lapandic, Dzenan, et al.
(författare)
-
Aperiodic Communication for MPC in Autonomous Cooperative Landing
- 2021
-
Ingår i: IFAC PAPERSONLINE. - : Elsevier BV. - 2405-8963. ; , s. 113-118
-
Konferensbidrag (refereegranskat)abstract
- This paper investigates the rendezvous problem for the autonomous cooperative landing of an unmanned aerial vehicle (UAV) on an unmanned surface vehicle (USV). Such heterogeneous agents, with nonlinear dynamics, are dynamically decoupled but share a common cooperative rendezvous task. The underlying control scheme is based on distributed Model Predictive Control (MPC). The main contribution is a rendezvous algorithm with an online update rule of the rendezvous location. The algorithm only requires the agents to exchange information when they can not guarantee to rendezvous. Hence, the exchange of information occurs aperiodically, which reduces the necessary communication between the agents. Furthermore, we prove that the algorithm guarantees recursive feasibility. The simulation results illustrate the effectiveness of the proposed algorithm applied to the problem of autonomous cooperative landing.
|
|
| 36. |
- Lindh, Linnea, et al.
(författare)
-
Photophysics and photochemistry of iron carbene complexes for solar energy conversion and photocatalysis
- 2020
-
Ingår i: Catalysts. - : MDPI AG. - 2073-4344. ; 10:3
-
Forskningsöversikt (refereegranskat)abstract
- Earth-abundant first row transition metal complexes are important for the development of large-scale photocatalytic and solar energy conversion applications. Coordination compounds based on iron are especially interesting, as iron is the most common transition metal element in the Earth’s crust. Unfortunately, iron-polypyridyl and related traditional iron-based complexes generally suffer from poor excited state properties, including short excited-state lifetimes, that make them unsuitable for most light-driven applications. Iron carbene complexes have emerged in the last decade as a new class of coordination compounds with significantly improved photophysical and photochemical properties, that make them attractive candidates for a range of light-driven applications. Specific aspects of the photophysics and photochemistry of these iron carbenes discussed here include long-lived excited state lifetimes of charge transfer excited states, capabilities to act as photosensitizers in solar energy conversion applications like dye-sensitized solar cells, as well as recent demonstrations of promising progress towards driving photoredox and photocatalytic processes. Complementary advances towards photofunctional systems with both Fe(II) complexes featuring metal-to-ligand charge transfer excited states, and Fe(III) complexes displaying ligand-to-metal charge transfer excited states are discussed. Finally, we outline emerging opportunities to utilize the improved photochemical properties of iron carbenes and related complexes for photovoltaic, photoelectrochemical and photocatalytic applications.
|
|
| 37. |
|
|
| 38. |
- Lührs, Oscar, et al.
(författare)
-
Combining Indocyanine Green and Tc99-nanocolloid does not increase the detection rate of sentinel lymph nodes in early stage cervical cancer compared to Indocyanine Green alone
- 2019
-
Ingår i: Gynecologic Oncology. - : Elsevier BV. - 0090-8258.
-
Tidskriftsartikel (refereegranskat)abstract
- Objective: To investigate whether combining two independent tracers increases the SLN-detection rate in cervical cancer. Methods: Consecutive women with early stage cervical cancer planned for a robotic radical hysterectomy or a robotic radical trachelectomy with sentinel lymph node (SLN) detection were included. After cervical injections of Indocyanine green (ICG) and Tc99-nanocolloid (Tc99), near-infrared fluorescence imaging and a gamma probe were used to identify SLNs in the upper and lower paracervical pathways (UPP/LPP). A strict surgical algorithm was adhered to and the SLNs were defined as SLN-ICG, SLN-ICG+Tc99 or SLN-Tc99. In FIGO-stage ≥IA2 cancers a full pelvic lymph node dissection (PLND) was performed after detection of SLNs. The primary endpoint was the SLN detection rate per tracer and combination of tracers. Secondary endpoints were sensitivity and mapping rates of the SLN algorithm per tracer and combination of tracers. Results: In the sixty-five analyzed women, the bilateral mapping rate was 98.5% for ICG and 60% for Tc99 (p < 0.01). Combining the tracers did not increase the bilateral detection rate. In three women (5%) Tc99 identified ICG-negative non-metastatic SLNs without impact on the bilateral detection rate. Eight women (12%) had lymph node metastases (LNMs), all had at least one metastatic SLN. Seven (35%) of the 20 metastatic SLNs were detected by ICG only and 12 (60%) were ICG and Tc99 positive. Conclusion: SLN detection rate was significantly higher using ICG compared with Tc99. ICG identified all patients with LNMs. Combining ICG and Tc99 did not improve the bilateral detection rate of SLNs.
|
|
| 39. |
- Lührs, Oscar, et al.
(författare)
-
Resection of the upper paracervical lymphovascular tissue should be an integral part of a pelvic sentinel lymph node algorithm in early stage cervical cancer
- 2021
-
Ingår i: Gynecologic Oncology. - : Elsevier BV. - 0090-8258. ; 163:2, s. 289-293
-
Tidskriftsartikel (refereegranskat)abstract
- Objective: To investigate the prevalence of lymph nodes and lymph node metastases (LNMs) in the upper paracervical lymphovascular tissue (UPLT) in early stage cervical cancer. Methods: In this prospective study consecutive women with stage IA1-IB1 cervical cancer underwent a pelvic lymphadenectomy including identification of sentinel nodes (SLNs) as part of a nodal staging procedure in conjunction with a robotic radical hysterectomy (RRH) or robotic radical trachelectomy (RRT). Indocyanine green (ICG) was used as tracer. The UPLT was separately removed and defined as “SLN-parametrium” and, as all SLN tissue, subjected to ultrastaging and immunohistochemistry. Primary endpoint was prevalence of lymph nodes and metastatic lymph nodes in the UPLT. Secondary endpoints were complications associated with removal of the UPLT. Results: One hundred and forty-five women were analysed. Nineteen (13.1%) had pelvic LNMs, all identified by at least one metastatic SLN. In 76 women (52.4%) at least one UPLT lymph node was identified. Metastatic UPLT lymph nodes were identified in six women of which in three women (2.1% of all women and 15.8% of node positive women) without lateral pelvic LNMs. Thirteen women had lateral pelvic SLN LNMs with either no (n = 5) or benign (n = 8) UPLT lymph nodes. No intraoperative complications occurred due to the removal of the UPLT. Conclusion: Removal of the UPLT should be an integral part of the SLN concept in early stage cervical cancer.
|
|
| 40. |
- Lührs, Oscar, et al.
(författare)
-
Similar distribution of pelvic sentinel lymph nodes and nodal metastases in cervical and endometrial cancer. A prospective study based on lymphatic anatomy
- 2022
-
Ingår i: Gynecologic Oncology. - : Elsevier BV. - 0090-8258. ; 165:3, s. 466-471
-
Tidskriftsartikel (refereegranskat)abstract
- Objective: Comparing the anatomical distribution of metastatic and non-metastatic pelvic sentinel lymph nodes (SLN) in cervical and endometrial cancer. Methods: Detailed SLN mapping results were prospectively retrieved in cervical (n = 145) or high-risk endometrial cancer (n = 201) patients undergoing a robotic staging procedure. Cervically injected Indocyanine Green (ICG), allowing for reinjection in case of inadequate mapping, was used as tracer. An anatomically based definition of SLNs was adhered to evaluating the upper (UPP) and lower (LPP) paracervical lymphatic pathways. The positions of SLNs were intraoperatively depicted on an anatomical chart. A completory pelvic lymphadenectomy was performed. Mapping rates and anatomical distribution of SLNs and the location of pelvic nodal metastases were compared between groups. Results: The bilateral mapping rate was 97.9% and 95.0% for cervical and endometrial cancer respectively (p = .16). The proportion of typically positioned (interiliac and proximal obturator fossa) SLNs along the UPP was similar between groups (78.1% vs 82.1%, p = .09), and the rate of metastatic SLNs in the obturator fossa was 54.1% and 48.6% respectively (p = .45). All pelvic node positive women (cervical cancer n = 19, endometrial cancer n = 37) had at least one metastatic SLN. Anatomically typical positions could not be defined along the LPP. Conclusion: The anatomical location of SLNs and SLN metastases are similar in cervical and endometrial cancer suggesting that sensitivity results for an SLN concept in endometrial cancer and cervical cancer can be accumulated.
|
|
| 41. |
- Muskardin, Tin, et al.
(författare)
-
A Novel Landing System to Increase Payload Capacity and Operational Availability of High Altitude Long Endurance UAVs
- 2017
-
Ingår i: Journal of Intelligent and Robotic Systems. - : SPRINGER. - 0921-0296 .- 1573-0409. ; 88:2-4, s. 597-618
-
Tidskriftsartikel (refereegranskat)abstract
- Unmanned stratospheric aircraft capable of staying aloft for long periods of time have become a topic of interest in the past years. Several problems are still to be solved to allow for a profitable commercial use of such aircraft. The inherent lightweight design leads to fragile structures with low payload capacities and a high wind sensitivity. The weather dependence significantly reduces the system's operational availability. To address these drawbacks a novel landing system is proposed in this paper. The landing gear can be removed from the aircraft and a ground-based mobile landing platform is introduced. The main technical challenges consist in the precise relative state estimation and cooperative control of the involved vehicles. A reliable simulation model of the overall system was developed and a number of simulation experiments performed before the actual landing was attempted with an experimental system setup. Multiple successful landing experiments demonstrate the validity of the proposed system.
|
|
| 42. |
- Nissling, Linnea, 1991, et al.
(författare)
-
Effects of patient-driven iCBT for anxiety in routine primary care and the relation between increased experience of empowerment and outcome : A randomized controlled trial
- 2021
-
Ingår i: Internet Interventions. - : Elsevier. - 2214-7829. ; 26
-
Tidskriftsartikel (refereegranskat)abstract
- Background: The World Health Organization has declared that primary care should be organized to empower individuals, families, and communities to optimize health. Internet cognitive behavioral therapy (iCBT) tailored by psychologists' initial assessments to meet patients' specific needs have shown promising effects. However, few studies have evaluated patient involvement in decisions during iCBT. Aim: This study aimed to explore the effect of patient-driven iCBT compared to standard iCBT on perceived control over treatment, adherence, and level of anxiety symptoms. A secondary aim was to assess the relationship between changes in empowerment and changes in anxiety symptoms. Method: Participants were patients recruited form primary care and assessed as meeting the criterion for an anxiety disorder. Participants were randomized to patient-driven iCBT (n = 27) or standard iCBT (n = 28). Patient-driven iCBT was adapted to participants' preferences regarding for example focus of treatment program and order of modules. Participants randomized to the control condition received the standard iCBT program for anxiety disorders at the participating unit. The outcome measures were patients' perceived control over treatment, adherence to treatment, symptoms of anxiety, depression and general disability as well as the experience of empowerment. Results: Participants in patient-driven iCBT had statistically higher perceived control over treatment (t(43) = 2.13, p = .04). Symptoms were significantly reduced in both arms with regards to anxiety, depression, and general disability. A significant time per condition interaction effect for anxiety symptoms was observed (df = 45.0; F = 3.055; p = .038), where the patient-driven condition had a significantly larger reduction in anxiety. For both groups a significant correlation of r = 0.47 was found between changes in empowerment and changes in anxiety. Conclusion: Results indicate that iCBT that is patient-driven, may have a greater effect on anxiety, than standard iCBT. The effect on perceived control over treatment might also be larger in patient-driven treatments than in standard iCBT. Internet-based therapies inherently promote as active agents of their own care and might be well suited for promoting perceived control and empowerment. Findings need to be replicated given the small sample size and the explorative nature of the study.
|
|
| 43. |
|
|
| 44. |
- Persson Asplund, Robert, et al.
(författare)
-
Internet-based stress management for distressed managers : results from a randomised controlled trial
- 2018
-
Ingår i: Occupational and Environmental Medicine. - : BMJ. - 1351-0711 .- 1470-7926. ; 75:2, s. 105-113
-
Tidskriftsartikel (refereegranskat)abstract
- Objective The aim of this randomised controlled trial (RCT) was to evaluate the efficacy of a guided internet-based stress management intervention (iSMI) among distressed managers compared with a attention control group (AC) with full access to treatment-as-usual.Method A total sample of 117 distressed managers, mainly employed in the healthcare, IT, communication and educational sector, were randomised to either iSMI (n=59) or an AC group (n=58). The iSMI consisted of eight modules including cognitive behavioural stress management and positive management techniques. Participants received a minimal and weekly guidance from a psychologist or master-level psychology student focusing on support, feedback and adherence to the intervention. Self-report data were assessed at pre, post and 6 months after the intervention. The primary outcome was perceived stress (Perceived Stress Scale-14). The secondary outcomes included mental and work-related health outcomes.Results Participants in the iSMI intervention reported significantly less symptoms of perceived stress (d=0.74, 95% CI 0.30 to 1.19) and burnout (d=0.95, 95% CI 0.53 to 1.37) compared with controls, at postassessment. Significant medium-to-large effect sizes were also found for depression, insomnia and job satisfaction. Long-term effects (6 months) were seen on the mental health outcomes.Conclusion This is one of the first studies showing that iSMIs can be an effective, accessible and potentially time-effective approach of reducing stress and other mental-related and work-related health symptoms among distressed managers. Future studies are needed addressing distressed managers and the potential of indirect effects on employee stress and satisfaction at work.
|
|
| 45. |
- Persson, Carina Ulla, 1970, et al.
(författare)
-
Determinants of Stroke in a General Male Population : Forty-Eight Year Time-Dependent Updated Follow-Up of the Study of Men Born in 1913
- 2018
-
Ingår i: Stroke. - : LIPPINCOTT WILLIAMS & WILKINS. - 0039-2499 .- 1524-4628. ; 49:12, s. 2830-2836
-
Tidskriftsartikel (refereegranskat)abstract
- Background and Purpose - To further improve preventive strategies against stroke, there is a need for epidemiological long-term studies. The study aimed at a prospective investigation of stroke determinants in the general male population.Methods - During a period of 48 years, from 50 to 98 years of age, a population-based sample of 854 men was followed using repeated medical examinations, lifestyle questionnaires, data from hospital records and the National Cause of Death Register.Results - Determinants of ischemic stroke were atrial fibrillation (hazard ratio [HR], 6.61; 95% CI, 4.47-9.77); mother dead from cardiovascular disease (HR, 1.53; 1.09-2.17); high education (HR, 0.81; 0.69-0.96); and high physical activity level during leisure time (HR, 0.68; 0.50-0.93). For hemorrhagic stroke heart rate (HR, 1.04; 1.01-1.06) and mother dead from stroke (HR, 3.56; 1.43-8.87) constituted an increased risk. Statistically significant determinants for all stroke were atrial fibrillation (HR, 5.34; 3.68-7.75); high diastolic blood pressure (HR, 1.02; 1.01-1.03); high body weight (HR, 0.96; 0.94-0.99); high educational level (HR, 0.79; 0.68-0.92); wide waist circumference (HR, 1.04; 1.01-1.07); smoking (HR, 1.25; 1.06-1.48); mother dead from cerebrovascular disease (HR, 1.43; 1.05-1.94); and diabetes mellitus (HR, 1.65; 1.02-2.68). Of all men diagnosed with atrial fibrillation, 88% had a stroke during follow-up.Conclusions - Atrial fibrillation was by far the strongest determinant of stroke during 48 years of follow-up in a male population sample followed until the age of 98 years. The results warrant improved prophylaxis through intense treatment of modifiable determinants.
|
|
| 46. |
- Persson, Helena, et al.
(författare)
-
In Vitro Evolution of Antibodies Inspired by In Vivo Evolution
- 2018
-
Ingår i: Frontiers in Immunology. - : Frontiers Media S.A.. - 1664-3224. ; 9
-
Tidskriftsartikel (refereegranskat)abstract
- In vitro generation of antibodies often requires variable domain sequence evolution to adapt the protein in terms of affinity, specificity, or developability. Such antibodies, including those that are of interest for clinical development, may have their origins in a diversity of immunoglobulin germline genes. Others and we have previously shown that antibodies of different origins tend to evolve along different, preferred trajectories. Apart from substitutions within the complementary determining regions, evolution may also, in a germline gene-origin-defined manner, be focused to residues in the framework regions, and even to residues within the protein core, in many instances at a substantial distance from the antibody's antigen-binding site. Examples of such germline origin-defined patterns of evolution are described. We propose that germline gene-preferred substitution patterns offer attractive alternatives that should be considered in efforts to evolve antibodies intended for therapeutic use with respect to appropriate affinity, specificity, and product developability. We also hypothesize that such germline gene-origin-defined in vitro evolution hold potential to result in products with limited immunogenicity, as similarly evolved antibodies will be parts of conventional, in vivo-generated antibody responses and thus are likely to have been seen by the immune system in the past.
|
|
| 47. |
- Persson, Jan, et al.
(författare)
-
A prospective study evaluating an optimized sentinel node algorithm in early stage cervical cancer : The PROSACC-study
- 2024
-
Ingår i: Gynecologic Oncology. - 0090-8258. ; 187, s. 178-183
-
Tidskriftsartikel (refereegranskat)abstract
- Objective: A single center prospective non-randomized study to assess a systematically developed anatomically-based sentinel lymph node (SLN) algorithm in cervical cancer. Methods: Consecutive women with FIGO 2009 stage 1A2-2A1 cervical cancer undergoing robotic radical hysterectomy/trachelectomy between September 2014 and January 2023 had cervically injected Indocyanine Green (ICG) as a tracer for detection of pelvic SLN. An anatomically based surgical algorithm was adhered to; defining SLNs as the juxtauterine mapped nodes within the upper and lower paracervical lymphatic pathways including separate removal of the parauterine lymphovascular tissue (PULT). A completion pelvic lymphadenectomy was performed. Ultrastaging and immunohistochemistry was performed on SLNs, including the PULT. Results: 181 women were included for analysis. Median histologic tumor size was 14.0 mm (range 2–80 mm). The bilateral mapping rate was 98.3%. As per protocol an interim analysis rejected H0 and inclusion stopped at 29 node positive women, all identified by at least one metastatic ICG-defined SLN. One woman awaiting histology at study-closure was node positive and included in the analysis. Sensitivity was 100% (95% CI, 88.4%–100%) and NPV 100% (95% CI, 97.6%–100%). In node positive women, the proximal obturator position harbored 46.1% of all SLN metastases representing the only position in 40% and 10% had isolated metastases in the PULT. Conclusions: Strictly adhering to an anatomically based SLN-algorithm including identification of parallell lymphatics within major pathways, partilularly the obturator compartment, assessment of the PULT, restricting nodal dissection to the removal of SLNs accurately identifies pelvic nodal metastatic disease in early-stage cervical cancer.
|
|
| 48. |
- Persson, Linnea, et al.
(författare)
-
An optimization algorithm based on forward recursion with applications to variable horizon MPC
- 2024
-
Ingår i: European Journal of Control. - : ELSEVIER. - 0947-3580 .- 1435-5671. ; 75
-
Tidskriftsartikel (refereegranskat)abstract
- We consider optimization algorithms designed for variable horizon model predictive control. Traditionally, such problems are considered intractable for real-time applications that require fast computations, as they need to solve multiple optimal control problems with varying horizons at each sampling instance. The main contribution is an algorithm that efficiently solves multiple optimal control problems with different prediction horizons in a recursive manner. This algorithm has been successfully implemented and integrated into the OSQP solver, resulting in a real-time controller that is both fast and reliable. To assess the effectiveness of the approach, we conducted evaluations in both a realistic simulation environment and on real hardware during outdoor flight experiments. Specifically, we focused on two distinct rendezvous maneuvers for autonomous landings of unmanned aerial vehicles. The results obtained from these evaluations further validate the practicality and efficacy of the proposed algorithm. (c) 2023 The Author(s). Published by Elsevier Ltd on behalf of European Control Association. This is an open access article under the CC BY license ( http://creativecommons.org/licenses/by/4.0/ )
|
|
| 49. |
- Persson, Linnea, 1992-
(författare)
-
Autonomous and Cooperative Landings Using Model Predictive Control
- 2019
-
Licentiatavhandling (övrigt vetenskapligt/konstnärligt)abstract
- Cooperation is increasingly being applied in the control of interconnected multi-agent systems, and it introduces many benefits. In particular, cooperation can improve the efficiency of many types of missions, and adds flexibility and robustness against external disturbances or unknown obstacles. This thesis investigates cooperative maneuvers for aerial vehicles autonomously landing on moving platforms, and how to safely and robustly perform such landings on a real system subject to a variety of disturbances and physical and computational constraints. Two specific examples are considered: the landing of a fixed-wing drone on top of a moving ground carriage; and the landing of a quadcopter on a boat. The maneuvers are executed in a cooperative manner where both vehicles are allowed to take actions to reach their common objective while avoiding safety based spatial constraints. Applications of such systems can be found in, for example, autonomous deliveries, emergency landings, and search and rescue missions. Particular challenges of cooperative landing maneuvers include the heterogeneous and nonlinear dynamics, the coupled control, the sensitivity to disturbances, and the safety criticality of performing a high-velocity landing maneuver.The thesis suggests the design of a cooperative control algorithm for performing autonomous and cooperative landings. The algorithm is based on model predictive control, an optimization-based method where at every sampling instant a finite-horizon optimal control problem is solved. The advantages of applying this control method in this setting arise from its ability to include explicit dynamic equations, constraints, and disturbances directly in the computation of the control inputs. It is shown how the resulting optimization problem of the autonomous landing controller can be decoupled into a horizontal and a vertical sub-problem, a finding which significantly increases the efficiency of the algorithm. The algorithm is derived for two different autonomous landing systems, which are subsequently implemented in realistic simulations and on a drone for real-world flight tests. The results demonstrate both that the controller is practically implementable on real systems with computational limitations, and that the suggested controller can successfully be used to perform the cooperative landing under the influence of external disturbances and under the constraint of various safety requirements.
|
|
| 50. |
- Persson, Linnea, et al.
(författare)
-
Cooperative Rendezvous of Ground Vehicle and Aerial Vehicle using Model Predictive Control
- 2017
-
Ingår i: 2017 IEEE 56TH ANNUAL CONFERENCE ON DECISION AND CONTROL (CDC). - : IEEE. - 9781509028733 ; , s. 2819-2824
-
Konferensbidrag (refereegranskat)abstract
- This paper considers the problem of controlling a fixed-wing unmanned aerial vehicle and a cooperating unmanned ground vehicle to rendezvous by making the aerial vehicle land on top the ground vehicle. Both vehicles are actively taking part in the control effort, where they coordinate positions and velocities to complete the landing. The rendezvous time and the terminal state are kept free to increase the flexibility of the solution. There are two main challenges with this maneuver. First, the controller must force the system to stay within a safe set such that the aerial vehicle approaches the ground vehicle directly from above. Second, the rendezvous must occur within some finite distance. A model predictive control algorithm is proposed to achieve these objectives. The choice is motivated by recent experimental results showing how the landing safety and efficiency could benefit from including safety margins already in the computation of the control inputs. A controller, which steers the agents towards rendezvous and which indirectly provides safety guarantees through non-convex optimization constraints, is derived. Simulations are provided showing the ability of the controller to plan a safe trajectory online, even under the disturbance of wind gusts.
|
|
