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- Moskalenko, R. A., et al.
(författare)
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Involvement of proinflammatory S100A9/A8 in the atherocalcinosis of aortic valves
- 2017
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Ingår i: Pathologia. - : Zaporizʹkyĭ derz͡havnyĭ medychnyĭ universytet. - 2306-8027. ; :1, s. 49-56
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Tidskriftsartikel (refereegranskat)abstract
- According to the results of the Euro-Heart Survey on Vascular Heart Disease the most common pathology is nonrheumatic aortic stenosis, it is also called as calcific aortic valve stenosis (CAVS), as in its pathogenesis the process of biomineralization of valve cusps and ring plays the main role. The aim of the work is the immunohistochemical study of mineralized tissue of aortic heart valves, which are affected by atherocalcinosis. Materials and methods. 30 samples of mineralized aortic valves (I group) and 10 samples of aortic valve without evidence of biomineralization (II group - control) were studied. Immunohistochemical study of expression of collagen (Collagen I), CD68, myeloperoxidase (MPO), calgranulin A (S100A8), calgranulin B (8100A9), caspase 3 (Casp 3) and osteopontin (OPN) was conducted in AV tissue of both groups. Results. In CAV tissues the fibrillar component (collagen I) growths was found, but the quantitative and qualitative compositions of CD68+ circulating inflammatory cells are not significantly different from the control group. CAVs contain much more MPO+-cells (p <0.001) in comparison to the group of AVs without biomineralization. Our data show a significant increase of the S100A9 and OPN expression in the mineralized tissue of AVs (p <0.01). Also, a higher expression level of Casp3 and MPO was found in CAVs (p <0.05). Comparing the first and the second groups of AVs connection between the expression of S100A8 was not determined. Conclusion. High Casp 3 expression confirms the increased level of cell elimination in the CAVs tissue, which is obviously connected with the impact of high local concentrations of S100A9. These facts can contribute to the development of pathological biomineralization of AV. Since osteopontin inhibits the hydroxyapatite formation by binding to the surface of the crystals, its hyperproduction is a counteracting factor against biomineralization in AV tissue.
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| 3. |
- Sikora, K., et al.
(författare)
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Heavy metals effect on the rat uterus and effectiveness of vitamin E treatment
- 2023
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Ingår i: Jordan Journal of Biological Sciences. - : Hashemite University. - 1995-6673. ; 16:3, s. 455-465
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Tidskriftsartikel (refereegranskat)abstract
- Environmental pollution by heavy metals (HMs) is an increasingly critical problem that is posing a growing threat to reproductive health. Consequently, the aim of the current research was to study changes in rat uterus under 90 days of HMs exposure and estimate the efficacy and benefits of vitamin E treatment. Female rats were randomly divided into three groups: untreated animals (control group); animals orally treated with the HMs mixture (HM group); and animals treated simultaneously with HMs and vitamin E (HM+E group). The toxic effects of the HMs (comprising Zn, Cu, Mn, Fe, Pb, and Cr) on the uterus of rats were investigated by histological, morphometrical, spectrophotometrical, and statistical methods.Long-term HMs exposure triggered pathological (degenerative, inflammation, and atrophic) changes in the rat uterus together with a significant reduction of the uterine-wall thickness (37.99%, p<0.0001) compared to the control. In contrast, there was a lower intensity of morphological lesions and wall thickness decrease (26.03%, p<0.0001) in the uterus, in rats that underwent treatment with vitamin E. A substantial bioaccumulation of zinc, copper, manganese, iron, lead, and chromium general levels in the rat uterus was demonstrated in both the HM group (74.46%, p<0.0001) and the HM+E group (49.81%, p<0.0001), as compared to the control group. The lowest accumulative potential belonged to Zn and the highest to Pb. The results obtained showed a significant decline in the weight of animals treated by HMs in both HM (18,21%, p<0.01) and HM+E (13,09%; p<0.05) groups compared to the control. Our findings have demonstrated that treatment with vitamin E in HM-induced intoxication has a significant restrain of HMs accumulation (up to 16.46%, p<0.0001) together with morphometric variations (less on 16.17%, p<0.01).In summary, long-term exposure to the HMs mixture had a pernicious toxic effect on the morphology and chemical content of the uterus of rats (strong negative correlations). Treatment with vitamin E significantly reversed the HMs impact on the uterus but did not demonstrate absolute protection.
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- Denysenko, A.P., et al.
(författare)
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A comprehensive study of dura mater biomineralization : morphological, crystallographic, and immunohistochemical aspects
- 2023
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Ingår i: Reports of Morphology. - : Vinnytsia National Pyrogov Memorial Medical University. - 1818-1295 .- 2616-6194. ; 29:4, s. 50-57
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Tidskriftsartikel (refereegranskat)abstract
- Biomineralization is a process of formation of biominerals widespread among living organisms. This phenomenon occurs in the central nervous system in normal and pathological conditions. Typically, this can manifest age-related changes, and the prevalence of biominerals increases with age. At the same time, it can be a sign of pathology-tumour growth, dystrophy, metabolic disorders, etc. This work aims to study the morphological features of the dura mater with signs of biomineralization. In this work, we examined 30 samples of the dura mater with signs of biomineralization (group I) and 30 samples without these signs (group II) obtained during autopsies in the pathology department of the Sumy Regional Clinical Hospital. For the morphological charact erist ics of the dura mat er, we used hi stologi cal, hi stochemi cal and immunohistochemical methods, as well as scanning electron microscopy. Statistical processing of the results of the immunohistochemical study was carried out in the GraphPad Prism 8.0 statistical package using parametric and non-parametric research methods. To divide the samples into groups, we relied on the histological method-staining with hematoxylin-eosin. Histochemical methods (alizarin red staining, von Koss method) confirmed the presence of calcium compounds in the studied group. Van Gieson staining visualized the collagen fibres of the dura mater, and the PAS reaction di d not reveal the presence of gl ycosaminogl ycans in the mineral deposit s. Immunohistochemical examination of the dura mater with antibodies against osteopontin revealed a significant difference between the study and control groups (p<0.001), confirming the critical role of osteopontin in the biomineralization process in this tissue. With the help of scanning electron microscopy, it was found that biominerals in the dura mater are represented by tens to hundreds of formations of various shapes (rounded, oval and irregular), varying from tens of nanometers to 50 micrometres. Their composition was analyzed using maps and spectra of energy-dispersive X-ray spectroscopy and most probably corresponded to calcium hydroxyapatite. So, the dura mater is characterized by biomineralization, manifested by the formation of microscopic calcium hydroxyapatite composites.
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- Okoye, Jennifer C., et al.
(författare)
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Ferric quinate (QPLEX) inhibits the interaction of major outer membrane protein (MOMP) with the Lewis b (Leb) antigen and limits Campylobacter colonization in broilers
- 2023
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Ingår i: Frontiers in Microbiology. - : Frontiers Media S.A.. - 1664-302X. ; 14
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Tidskriftsartikel (refereegranskat)abstract
- Campylobacter jejuni colonizes hosts by interacting with Blood Group Antigens (BgAgs) on the surface of gastrointestinal epithelia. Genetic variations in BgAg expression affects host susceptibility to C. jejuni. Here, we show that the essential major outer membrane protein (MOMP) of C. jejuni NCTC11168 binds to the Lewis b (Leb) antigen on the gastrointestinal epithelia of host tissues and this interaction can be competitively inhibited by ferric quinate (QPLEX), a ferric chelate structurally similar to bacterial siderophores. We provide evidence that QPLEX competitively inhibits the MOMP-Leb interaction. Furthermore, we demonstrate that QPLEX can be used as a feed additive in broiler farming to significantly reduce C. jejuni colonization. Our results indicate that QPLEX can be a viable alternative to the preventative use of antibiotics in broiler farming to combat C. jejuni infections.
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