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- Benetton, M., et al.
(författare)
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Molecular Measurable Residual Disease Assessment before Hematopoietic Stem Cell Transplantation in Pediatric Acute Myeloid Leukemia Patients: A Retrospective Study by the I-BFM Study Group
- 2022
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Ingår i: Biomedicines. - : MDPI AG. - 2227-9059. ; 10:7
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Tidskriftsartikel (refereegranskat)abstract
- Hematopoietic stem cell transplantation (HSCT) is a curative post-remission treatment in patients with acute myeloid leukemia (AML), but relapse after transplant is still a challenging event. In recent year, several studies have investigated the molecular minimal residual disease (qPCR-MRD) as a predictor of relapse, but the lack of standardized protocols, cut-offs, and timepoints, especially in the pediatric setting, has prevented its use in several settings, including before HSCT. Here, we propose the first collaborative retrospective I-BFM-AML study assessing qPCR-MRD values in pretransplant bone marrow samples of 112 patients with a diagnosis of AML harboring t(8;21)(q22; q22)RUNX1::RUNX1T1, or inv(16)(p13q22)CBFB::MYH11, or t(9;11)(p21;q23)KMT2A::MLLT3, or FLT3-ITD genetic markers. We calculated an ROC cut-off of 2.1 x 10(-4) that revealed significantly increased OS (83.7% versus 57.1%) and EFS (80.2% versus 52.9%) for those patients with lower qPCR-MRD values. Then, we partitioned patients into three qPCR-MRD groups by combining two different thresholds, 2.1 x 10(-4) and one lower cut-off of 1 x 10(-2), and stratified patients into low-, intermediate-, and high-risk groups. We found that the 5-year OS (83.7%, 68.6%, and 39.2%, respectively) and relapse-free survival (89.2%, 73.9%, and 67.9%, respectively) were significantly different independent of the genetic lesion, conditioning regimen, donor, and stem cell source. These data support the PCR-based approach playing a clinical relevance in AML transplant management.
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- Colledani, M., et al.
(författare)
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Analytical methods to support continuous improvements at Scania
- 2010
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Ingår i: International Journal of Production Research. - : Informa UK Limited. - 0020-7543 .- 1366-588X. ; 48:7, s. 1913-1945
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Tidskriftsartikel (refereegranskat)abstract
- Considerable effort is usually devoted by companies to ensure the competitiveness of their manufacturing systems. This makes continuous improvement a central topic in production management activities. Less attention is given to the methods that drive improvement and to the way actions are defined and selected. In the literature, analytical models and simulation are the most widely used tools for manufacturing systems' performance evaluation and improvement. In practice, simple approaches, mainly based on experience, seems to be the best compromise to face this problem. In a collaboration project between Politecnico di Milano ( Milano, Italy), Kungliga Tekniska hogskolan ( Stockholm, Sweden) and Scania CV AB (Sodertalje, Sweden) within the European Network of Excellence VRL-KCiP we proposed a new methodology, based on analytical methods, to support the company in manufacturing system productivity improvement through re-configuration. The application of this approach to the Scania six-cylinder engine-block machining line enabled a remarkable increment in throughput by selecting analytically the most suitable improvement actions.
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