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- Lehner, B.A.E., et al.
(författare)
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End-to-end mission design for microbial ISRU activities as preparation for a moon village
- 2019
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Ingår i: Acta Astronautica. - : Elsevier. - 0094-5765 .- 1879-2030. ; 162, s. 216-226
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Tidskriftsartikel (refereegranskat)abstract
- In situ resource utilization (ISRU) increasingly features as an element of human long-term exploration and settlement missions to the lunar surface. In this study, all requirements to test a novel, biological approach for ISRU are validated, and an end-to-end mission architecture is proposed. The general mission consists of a lander with a fully autonomous bioreactor able to process lunar regolith and extract elemental iron. The elemental iron could either be stored or directly utilized to generate iron wires or construction material. To maximize the success rate of this mission, potential landing sites for future missions are studied, and technical details (thermal radiation, shielding, power-supply) are analyzed. The final section will assess the potential mission architecture (orbit, rocket, lander, timeframe). This design might not only be one step further towards an international “Moon Village”, but may also enable similar missions to ultimately colonize Mars and further explore our solar system.
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- Olive, M., et al.
(författare)
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New cardiac and skeletal protein aggregate myopathy associated with combined MuRF1 and MuRF3 mutations
- 2015
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Ingår i: Human Molecular Genetics. - : Oxford University Press (OUP). - 0964-6906 .- 1460-2083. ; 24:13, s. 3638-3650
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Tidskriftsartikel (refereegranskat)abstract
- Protein aggregate myopathies (PAMs) define muscle disorders characterized by protein accumulation in muscle fibres. We describe a new PAM in a patient with proximal muscle weakness and hypertrophic cardiomyopathy, whose muscle fibres contained inclusions containing myosin and myosin-associated proteins, and aberrant distribution of microtubules. These lesions appear as intact A- and M-bands lacking thin filaments and Z-discs. These features differ from inclusions in myosin storage myopathy (MSM), but are highly similar to those in mice deficient for the muscle-specific RING finger proteins MuRF1 and MuRF3. Sanger sequencing excluded mutations in the MSM-associated gene MYH7 but identified mutations in TRIM63 and TRIM54, encoding MuRF1 and MuRF3, respectively. No mutations in other potentially disease-causing genes were identified by Sanger and whole exome sequencing. Analysis of seven family members revealed that both mutations segregated in the family but only the homozygous TRIM63 null mutation in combination with the heterozygous TRIM54 mutation found in the proband caused the disease phenotype. Both MuRFs are microtubule-associated proteins localizing to sarcomeric M-bands and Z-discs. They are E3 ubiquitin ligases that play a role in degradation of sarcomeric proteins, stabilization of microtubules and myogenesis. Lack of ubiquitin and the 20S proteasome subunit in the inclusions found in the patient suggested impaired turnover of thick filament proteins. Disruption of microtubules in cultured myotubes was rescued by transient expression of wild-type MuRF1. The unique features of this novel myopathy point to defects in homeostasis of A-band proteins in combination with instability of microtubules as cause of the disease.
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- Solanki, AK, et al.
(författare)
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Cobalt containing glass fibres and their synergistic effect on the HIF-1 pathway for wound healing applications
- 2023
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Ingår i: Frontiers in bioengineering and biotechnology. - : Frontiers Media SA. - 2296-4185. ; 11, s. 1125060-
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Tidskriftsartikel (refereegranskat)abstract
- Introduction and Methods: Chronic wounds are a major healthcare problem, but their healing may be improved by developing biomaterials which can stimulate angiogenesis, e.g. by activating the Hypoxia Inducible Factor (HIF) pathway. Here, novel glass fibres were produced by laser spinning. The hypothesis was that silicate glass fibres that deliver cobalt ions will activate the HIF pathway and promote the expression of angiogenic genes. The glass composition was designed to biodegrade and release ions, but not form a hydroxyapatite layer in body fluid.Results and Discussion: Dissolution studies demonstrated that hydroxyapatite did not form. When keratinocyte cells were exposed to conditioned media from the cobalt-containing glass fibres, significantly higher amounts of HIF-1α and Vascular Endothelial Growth Factor (VEGF) were measured compared to when the cells were exposed to media with equivalent amounts of cobalt chloride. This was attributed to a synergistic effect of the combination of cobalt and other therapeutic ions released from the glass. The effect was also much greater than the sum of HIF-1α and VEGF expression when the cells were cultured with cobalt ions and with dissolution products from the Co-free glass, and was proven to not be due to a rise in pH. The ability of the glass fibres to activate the HIF-1 pathway and promote VEGF expression shows the potential for their use in chronic wound dressings.
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