| 1. |
- Klionsky, Daniel J., et al.
(författare)
-
Guidelines for the use and interpretation of assays for monitoring autophagy
- 2012
-
Ingår i: Autophagy. - : Informa UK Limited. - 1554-8635 .- 1554-8627. ; 8:4, s. 445-544
-
Forskningsöversikt (refereegranskat)abstract
- In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process vs. those that measure flux through the autophagy pathway (i.e., the complete process); thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from stimuli that result in increased autophagic activity, defined as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (in most higher eukaryotes and some protists such as Dictyostelium) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the field understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field.
|
|
| 2. |
- Warr, N., et al.
(författare)
-
The Miniball spectrometer
- 2013
-
Ingår i: European Physical Journal A. Hadrons and Nuclei. - : Springer Science and Business Media LLC. - 1434-6001. ; 49:3
-
Tidskriftsartikel (refereegranskat)abstract
- The Miniball germanium detector array has been operational at the REX (Radioactive ion beam EXperiment) post accelerator at the Isotope Separator On-Line facility ISOLDE at CERN since 2001. During the last decade, a series of successful Coulomb excitation and transfer reaction studies have been performed with this array, utilizing the unique and high-quality radioactive ion beams which are available at ISOLDE. In this article, an overview is given of the technical details of the full Miniball setup, including a description of the.-ray and particle detectors, beam monitoring devices and methods to deal with beam contamination. The specific timing properties of the REX-ISOLDE facility are highlighted to indicate the sensitivity that can be achieved with the full Miniball setup. The article is finalized with a summary of some physics highlights at REX-ISOLDE and the utilization of the Miniball germanium detectors at other facilities.
|
|
| 3. |
- Bree, N, et al.
(författare)
-
Shape Coexistence in the Neutron-Deficient Even-Even Hg182-188 Isotopes Studied via Coulomb Excitation.
- 2014
-
Ingår i: Physical Review Letters. - 1079-7114. ; 112:16
-
Tidskriftsartikel (refereegranskat)abstract
- Coulomb-excitation experiments to study electromagnetic properties of radioactive even-even Hg isotopes were performed with 2.85 MeV/nucleon mercury beams from REX-ISOLDE. Magnitudes and relative signs of the reduced E2 matrix elements that couple the ground state and low-lying excited states in Hg182-188 were extracted. Information on the deformation of the ground and the first excited 0+ states was deduced using the quadrupole sum rules approach. Results show that the ground state is slightly deformed and of oblate nature, while a larger deformation for the excited 0+ state was noted in Hg182,184. The results are compared to beyond mean field and interacting-boson based models and interpreted within a two-state mixing model. Partial agreement with the model calculations was obtained. The presence of two different structures in the light even-mass mercury isotopes that coexist at low excitation energy is firmly established.
|
|
| 4. |
- Clément, E, et al.
(författare)
-
Spectroscopic Quadrupole Moments in ^{96,98}Sr: Evidence for Shape Coexistence in Neutron-Rich Strontium Isotopes at N=60.
- 2016
-
Ingår i: Physical Review Letters. - 1079-7114. ; 116:2
-
Tidskriftsartikel (refereegranskat)abstract
- Neutron-rich ^{96,98}Sr isotopes have been investigated by safe Coulomb excitation of radioactive beams at the REX-ISOLDE facility. Reduced transition probabilities and spectroscopic quadrupole moments have been extracted from the differential Coulomb excitation cross sections. These results allow, for the first time, the drawing of definite conclusions about the shape coexistence of highly deformed prolate and spherical configurations. In particular, a very small mixing between the coexisting states is observed, contrary to other mass regions where strong mixing is present. Experimental results have been compared to beyond-mean-field calculations using the Gogny D1S interaction in a five-dimensional collective Hamiltonian formalism, which reproduce the shape change at N=60.
|
|
| 5. |
- Gaffney, L. P., et al.
(författare)
-
Collectivity in the light radon nuclei measured directly via Coulomb excitation
- 2015
-
Ingår i: Physical Review C (Nuclear Physics). - 0556-2813. ; 91:6
-
Tidskriftsartikel (refereegranskat)abstract
- Background: Shape coexistence in heavy nuclei poses a strong challenge to state-of-the-art nuclear models, where several competing shape minima are found close to the ground state. A classic region for investigating this phenomenon is in the region around Z = 82 and the neutron midshell at N = 104. Purpose: Evidence for shape coexistence has been inferred from a-decay measurements, laser spectroscopy, and in-beam measurements. While the latter allow the pattern of excited states and rotational band structures to be mapped out, a detailed understanding of shape coexistence can only come from measurements of electromagnetic matrix elements. Method: Secondary, radioactive ion beams of Rn-202 and Rn-204 were studied by means of low-energy Coulomb excitation at the REX-ISOLDE in CERN. Results: The electric-quadrupole (E2) matrix element connecting the ground state and first excited 2(1)(+) state was extracted for both Rn-202 and Rn-204, corresponding to B(E2; 2(1)(+) -> 0(1)(+)) = 29(-8)(+8) and 43(-12)(+17) W.u., respectively. Additionally, E2 matrix elements connecting the 2(1)(+) state with the 4(1)(+) and 2(2)(+) states were determined in Rn-202. No excited 0(+) states were observed in the current data set, possibly owing to a limited population of second-order processes at the currently available beam energies. Conclusions: The results are discussed in terms of collectivity and the deformation of both nuclei studied is deduced to be weak, as expected from the low-lying level-energy schemes. Comparisons are also made to state-of-the-art beyond-mean-field model calculations and the magnitude of the transitional quadrupole moments are well reproduced.
|
|
| 6. |
- Van de Walle, J., et al.
(författare)
-
Coulomb excitation of neutron-rich Zn isotopes: First observation of the 2(1)(+) state in Zn-80
- 2007
-
Ingår i: Physical Review Letters. - 1079-7114. ; 99:14
-
Tidskriftsartikel (refereegranskat)abstract
- Neutron-rich, radioactive Zn isotopes were investigated at the Radioactive Ion Beam facility REX-ISOLDE (CERN) using low-energy Coulomb excitation. The energy of the 2(1)(+) state in Zn-78 could be firmly established and for the first time the 2(+)-> 0(1)(+) transition in Zn-80 was observed at 1492(1) keV. B(E2,2(1)(+)-> 0(1)(+)) values were extracted for Zn-74,Zn-76,Zn-78,Zn-80 and compared to large scale shell model calculations. With only two protons outside the Z=28 proton core, Zn-80 is the lightest N=50 isotone for which spectroscopic information has been obtained to date. Two sets of advanced shell model calculations reproduce the observed B(E2) systematics. The results for N=50 isotones indicate a good N=50 shell closure and a strong Z=28 proton core polarization. The new results serve as benchmarks to establish theoretical models, predicting the nuclear properties of the doubly magic nucleus Ni-78.
|
|
| 7. |
- Van de Walle, J., et al.
(författare)
-
Low-energy Coulomb excitation of neutron-rich zinc isotopes
- 2009
-
Ingår i: Physical Review C (Nuclear Physics). - 0556-2813. ; 79:1
-
Tidskriftsartikel (refereegranskat)abstract
- At the radioactive ion beam facility REX-ISOLDE, neutron-rich zinc isotopes were investigated using low-energy Coulomb excitation. These experiments have resulted in B(E2, 2(1)(+)-> 0(1)(+)) values in Zn74-80, B(E2, 4(1)(+)-> 2(1)(+)) values in Zn-74,Zn-76 and the determination of the energy of the first excited 2(1)(+) states in Zn-78,Zn-80. The zinc isotopes were produced by high-energy proton- (A = 74, 76, 80) and neutron-(A = 78) induced fission of U-238, combined with selective laser ionization and mass separation. The isobaric beam was postaccelerated by the REX linear accelerator and Coulomb excitation was induced on a thin secondary target, which was surrounded by the MINIBALL germanium detector array. In this work, it is shown how the selective laser ionization can be used to deal with the considerable isobaric beam contamination and how a reliable normalization of the experiment can be achieved. The results for zinc isotopes and the N = 50 isotones are compared to collective model predictions and state-of-the-art large-scale shell-model calculations, including a recent empirical residual interaction constructed to describe the present experimental data up to 2004 in this region of the nuclear chart.
|
|
| 8. |
- DiJulio, Douglas, et al.
(författare)
-
Coulomb excitation of In-107
- 2013
-
Ingår i: Physical Review C (Nuclear Physics). - 0556-2813. ; 87:1
-
Tidskriftsartikel (refereegranskat)abstract
- The radioactive isotope In-107 was studied using sub-barrier Coulomb excitation at the REX-ISOLDE facility at CERN. Two gamma rays were observed during the experiment, corresponding to the low-lying 11/2(+) and 3/2(-)states. The reduced transition probability of the 11/2(+) state was determined with the semiclassical Coulomb excitation code GOSIA2. The result is discussed in comparison to large-scale shell-model calculations, previous unified-model calculations, and earlier Coulomb excitation measurements in the odd-mass In isotopes. DOI: 10.1103/PhysRevC.87.017301
|
|
| 9. |
- DiJulio, Douglas, et al.
(författare)
-
Excitation strengths in Sn-109: Single-neutron and collective excitations near Sn-100
- 2012
-
Ingår i: Physical Review C (Nuclear Physics). - 0556-2813. ; 86:3
-
Tidskriftsartikel (refereegranskat)abstract
- A set of B(E2) values for the low-lying excited states in the radioactive isotope Sn-109 were deduced from a Coulomb excitation experiment. The 2.87-MeV/u radioactive beam was produced at the REX-ISOLDE facility at CERN and was incident on a secondary Ni-58 target. The B(E2) values were determined using the known 2(+) -> 0(+) reduced transition probability in Ni-58 as normalization with the semiclassical Coulomb excitation code GOSIA2. The transition probabilities are compared to shell-model calculations based on a realistic nucleon-nucleon interaction and the predictions of a simple core-excitation model. This measurement represents the first determination of multiple B(E2) values in a light Sn nucleus using the Coulomb excitation technique with low-energy radioactive beams. The results provide constraints for the single-neutron states relative to Sn-100 and also indicate the importance of both single-neutron and collective excitations in the light Sn isotopes.
|
|
| 10. |
- Ekström, Andreas, et al.
(författare)
-
Electric quadrupole moments of the 2(1)(+) states in Cd-100,Cd-102,Cd-104
- 2009
-
Ingår i: Physical Review C (Nuclear Physics). - 0556-2813. ; 80:5
-
Tidskriftsartikel (refereegranskat)abstract
- Using the REX-ISOLDE facility at CERN the Coulomb excitation cross sections for the 0(gs)(+)-> 2(1)(+) transition in the beta-unstable isotopes Cd-100,Cd-102,Cd-104 have been measured for the first time. Two different targets were used, which allows for the first extraction of the static electric quadrupole moments Q(2(1)(+)) in Cd-102,Cd-104. In addition to the B(E2) values in Cd-102,Cd-104, a first experimental limit for the B(E2) value in Cd-100 is presented. The data was analyzed using the maximum likelihood method. The provided probability distributions impose a test for theoretical predictions of the static and dynamic moments. The data are interpreted within the shell-model using realistic matrix elements obtained from a G-matrix renormalized CD-Bonn interaction. In view of recent results for the light Sn isotopes the data are discussed in the context of a renormalization of the neutron effective charge. This study is the first to use the reorientation effect for post-accelerated short-lived radioactive isotopes to simultaneously determine the B(E2) and the Q(2(1)(+)) values.
|
|
| 11. |
- Ilieva, S., et al.
(författare)
-
Coulomb excitation of neutron-rich Cd isotopes
- 2014
-
Ingår i: Physical Review C (Nuclear Physics). - 0556-2813. ; 89:1
-
Tidskriftsartikel (refereegranskat)abstract
- The isotopes (122),(124),Cd-126 were studied in a "safe" Coulomb-excitation experiment at the radioactive ion-beam facility REX-ISOLDE at CERN. The reduced transition probabilities B(E2; 0(g. s)(vertical bar) -> 2(1)(+)) and limits for the quadrupole moments of the first 2(+) excited states in the three isotopes were determined. The onset of collectivity in the vicinity of the Z = 50 and N = 82 shell closures is discussed by comparison with shell model and beyond mean-field calculations.
|
|
| 12. |
- Kumar, R., et al.
(författare)
-
ENHANCED 0(g.s.)(+) -> 2(1)(+) E2 TRANSITION STRENGTH IN Sn-112,Sn-114
- 2011
-
Ingår i: Acta Physica Polonica B. - 0587-4254. ; 42:3-4, s. 813-816
-
Konferensbidrag (refereegranskat)abstract
- The poorly known B(E2; 0(+) -> 2(+)) values of Sn-112 and Sn-114 have been measured to high precision. Two Coulomb excitation experiments were performed to determine the reduced transition probabilities relative to Sn-116 in order to minimize the systematic errors. The obtained B(E2 up arrow) values of 0.242(8) e(2)b(2) for Sn-112 and 0.232(8) e(2)b(2) for Sn-114 confirm the tendency of large B(E2 up arrow) values for the lighter tin isotopes below the midshell Sn-116 that has been observed recently in various radioactive ion beam experiments.
|
|
| 13. |
- Doornenbal, P., et al.
(författare)
-
Enhanced strength of the 2(1)(+) -> 0(g.s.)(+) transition in Sn-114 studied via Coulomb excitation in inverse kinematics
- 2008
-
Ingår i: Physical Review C (Nuclear Physics). - 0556-2813. ; 78:3
-
Tidskriftsartikel (refereegranskat)abstract
- The 2(1)(+) states of Sn-114,Sn-116 were excited in two consecutive experiments by means of Coulomb excitation in inverse kinematics on a Ni-58 target. A precise determination of the reduced transition probability B(E2; 0(g.s.)(+) -> 2(1)(+)) of Sn-114 relative to the well-known 2(1)(+) excitation strength in Sn-116 was achieved by comparing the relative projectile to target 2(1)(+) -> 0(g.s.)(+) decay intensities. The obtained B(E2 up arrow) value of 0.232(8)e(2)b(2) for Sn-114 confirms the tendency of large B(E2 up arrow) values for the light tin isotopes below the midshell Sn-116 that has been observed recently in various radioactive ion beam experiments. The result establishes most clearly the discrepancy between the current B(E2 up arrow) value predictions from large-scale shell-model calculations and the experimental deviation, which commences already for the stable Sn-114 isotope.
|
|
| 14. |
- Ekström, Andreas, et al.
(författare)
-
0(gs)+ -->2(1)+ transition strengths in 106Sn and 108Sn.
- 2008
-
Ingår i: Physical Review Letters. - 1079-7114. ; 101:1
-
Tidskriftsartikel (refereegranskat)abstract
- The reduced transition probabilities, B(E2; 0(gs)+ -->2(1)+), have been measured in the radioactive isotopes (108,106)Sn using subbarrier Coulomb excitation at the REX-ISOLDE facility at CERN. Deexcitation gamma rays were detected by the highly segmented MINIBALL Ge-detector array. The results, B(E2;0(gs)+ -->2(1)+)=0.222(19)e2b2 for 108Sn and B(E2; 0(gs)+-->2(1)+)=0.195(39)e2b2 for 106Sn were determined relative to a stable 58Ni target. The resulting B(E2) values are approximately 30% larger than shell-model predictions and deviate from the generalized seniority model. This experimental result may point towards a weakening of the N=Z=50 shell closure.
|
|
| 15. |
- Henning, G., et al.
(författare)
-
Exploring the stability of super heavy elements: First measurement of the fission barrier of 254No
- 2014
-
Ingår i: EPJ Web of Conferences. - : EDP Sciences. - 2101-6275 .- 2100-014X. ; 66
-
Konferensbidrag (refereegranskat)abstract
- The gamma-ray multiplicity and total energy emitted by the heavy nucleus 254No have been measured at 2 different beam energies. From these measurements, the initial distributions of spin I and excitation energy E * of 254No were constructed. The distributions display a saturation in excitation energy, which allows a direct determination of the fission barrier. 254No is the heaviest shell-stabilized nucleus with a measured fission barrier. © Owned by the authors, published by EDP Sciences, 2014.
|
|
| 16. |
- Al Jebali, Ramsey, et al.
(författare)
-
A helium gas scintillator active target for photoreaction measurements
- 2015
-
Ingår i: European Physical Journal A. Hadrons and Nuclei. - : Springer Science and Business Media LLC. - 1434-6001. ; 51:10
-
Tidskriftsartikel (refereegranskat)abstract
- A multi-cell He gas scintillator active target, designed for the measurement of photoreaction cross sections, is described. The target has four main chambers, giving an overall thickness of 0.103 g/cm(3) at an operating pressure of 2MPa. Scintillations are read out by photomultiplier tubes and the addition of small amounts of N-2 to the He, to shift the scintillation emission from UV to visible, is discussed. First results of measurements at the MAX IV Laboratory tagged-photon facility show that the target has a timing resolution of around 1 ns and can cope well with a high-flux photon beam. The determination of reaction cross sections from target yields relies on a Monte Carlo simulation, which considers scintillation light transport, photodisintegration processes in He-4, background photon interactions in target windows and interactions of the reaction-product particles in the gas and target container. The predictions of this simulation are compared to the measured target response.
|
|
| 17. |
- Cederkäll, Joakim, et al.
(författare)
-
Sub-Barrier Coulomb Excitation of ^110Sn and Its Implications for the ^100Sn Shell Closure
- 2007
-
Ingår i: Physical Review Letters. - 1079-7114. ; 98:17, s. 172501-
-
Tidskriftsartikel (refereegranskat)abstract
- The first excited 2+ state of the unstable isotope 110Sn has been studied in safe Coulomb excitation at 2.82 MeV/u using the MINIBALL array at the REX-ISOLDE post accelerator at CERN. This is the first measurement of the reduced transition probability of this state using this method for a neutron deficient Sn isotope. The strength of the approach lies in the excellent peak-to-background ratio that is achieved. The extracted reduced transition probability, B(E2:0+-->2+)=0.220±0.022e2b2, strengthens the observation of the evolution of the B(E2) values of neutron deficient Sn isotopes that was observed recently in intermediate-energy Coulomb excitation of 108Sn. It implies that the trend of these reduced transition probabilities in the even-even Sn isotopes is not symmetric with respect to the midshell mass number A=116 as 100Sn is approached.
|
|
| 18. |
- Ekström, Andreas, et al.
(författare)
-
Sub-barrier Coulomb excitation of Sn-106,Sn-108,Sn-110
- 2008
-
Ingår i: Frontiers in Nuclear structure, Astrophysics and Reactions - Finustar 2, AIP Conference Proceedings. - : AIP. - 0094-243X .- 1551-7616. ; 1012, s. 296-299
-
Konferensbidrag (refereegranskat)abstract
- The reduced transition probabilities between the first excited 2(+) state and the 0(+) ground state, B(E2; 0(+) -> 2(+)), have been measured in Sn-106,Sn-108,Sn-110 using sub-barrier Coulomb excitation in inverse kinematics at REX-ISOLDE. The results are, B(E2;0(+) -> 2(+)) = 0.220(22),0.226(17), and 0.228(32) e(2)b(2), for Sn-110, Sn-108, and Sn-106, respectively. The results for Sn-106,Sn-108 are preliminary. De-excitation gamma-rays were detected by the MINIBALL Ge-array. The B(E2) reveals detailed information about the nuclear wave function. A shell model prediction based on an effective CD-Bonn interaction in the nu(0g(7/2),2s, 1d, 0h(11/2)) model space using e(eff)(nu) =1.0 e follows the experimental values for the neutron rich Sn isotopes, but fails to reproduce the results presented here.
|
|
| 19. |
- Seidlitz, M., et al.
(författare)
-
Coulomb excitation of Mg-31
- 2011
-
Ingår i: Physics Letters. Section B: Nuclear, Elementary Particle and High-Energy Physics. - : Elsevier BV. - 0370-2693. ; 700:3-4, s. 181-186
-
Tidskriftsartikel (refereegranskat)abstract
- The ground state properties of Mg-31 indicate a change of nuclear shape at N = 19 with a deformed J(pi) = 1/2(+) intruder state as a ground state, implying that Mg-31 is part of the "island of inversion". The collective properties of excited states were the subject of a Coulomb excitation experiment at REX-ISOLDE, CERN, employing a radioactive Mg-31 beam. De-excitation gamma-rays were detected by the MINIBALL gamma-spectrometer in coincidence with scattered particles in a segmented Si-detector. The level scheme of Mg-3I was extended. Spin and parity assignment of the 945 keV state yielded 5/2(+) and its de-excitation is dominated by a strong collective M1 transition. Comparison of the transition probabilities of Mg-30,Mg-31,Mg-32 establishes that for the N = 19 magnesium isotope not only the ground state but also excited states are largely dominated by a deformed pf intruder configuration. (C) 2011 Elsevier B.V. All rights reserved.
|
|
| 20. |
|
|
| 21. |
|
|
| 22. |
|
|
| 23. |
- Ustun, Celalettin, et al.
(författare)
-
Hematopoietic stem-cell transplantation for advanced systemic mastocytosis
- 2014
-
Ingår i: Journal of Clinical Oncology. - 0732-183X .- 1527-7755. ; 32:29, s. 3264-3274
-
Tidskriftsartikel (refereegranskat)abstract
- PURPOSE: Advanced systemic mastocytosis (SM), a fatal hematopoietic malignancy characterized by drug resistance, has no standard therapy. The effectiveness of allogeneic hematopoietic stem-cell transplantation (alloHCT) in SM remains unknown.PATIENTS AND METHODS: In a global effort to define the value of HCT in SM, 57 patients with the following subtypes of SM were evaluated: SM associated with clonal hematologic non-mast cell disorders (SM-AHNMD; n = 38), mast cell leukemia (MCL; n = 12), and aggressive SM (ASM; n = 7). Median age of patients was 46 years (range, 11 to 67 years). Donors were HLA-identical (n = 34), unrelated (n = 17), umbilical cord blood (n = 2), HLA-haploidentical (n = 1), or unknown (n = 3). Thirty-six patients received myeloablative conditioning (MAC), and 21 patients received reduced-intensity conditioning (RIC).RESULTS: Responses in SM were observed in 40 patients (70%), with complete remission in 16 patients (28%). Twelve patients (21%) had stable disease, and five patients (9%) had primary refractory disease. Overall survival (OS) at 3 years was 57% for all patients, 74% for patients with SM-AHNMD, 43% for those with ASM, and 17% for those with MCL. The strongest risk factor for poor OS was MCL. Survival was also lower in patients receiving RIC compared with MAC and in patients having progression compared with patients having stable disease or response.CONCLUSION: AlloHCT was associated with long-term survival in patients with advanced SM. Although alloHCT may be considered as a viable and potentially curative therapeutic option for advanced SM in the meantime, given that this is a retrospective analysis with no control group, the definitive role of alloHCT will need to be determined by a prospective trial.
|
|
