| 1. |
- Taranova, L A, et al.
(författare)
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Comamonas testosteroni strain TI as a potential base for a microbial sensor detecting surfactants
- 2004
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Ingår i: Applied Biochemistry and Microbiology. - 0003-6838. ; 40:4, s. 404-408
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Tidskriftsartikel (refereegranskat)abstract
- Strain Comamonas testosteroni TI, capable of degrading the nonionic surfactant (NIS) nonylphenolethoxylate (OP-10), was used for constructing a pilot cellular biosensor. The lower NIS detection limit for the biosensor was 0.25 mg/l. We studied the substrate specificity of the biosensor with respect to a wide range of organic compounds: surfactants, polyaromatic compounds (PAC), carbohydrates, alcohols, etc. It was shown that the biosensor based on Comamonas testosteroni TI did not respond to glucose, which was an advantage over the formerly described biosensor based on Pseudomonas rathonis T. The amplitude of the sensor response remained stable for 10 days.
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| 2. |
- Indzhgiya, E. Yu, et al.
(författare)
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Interaction of Ferrocene Mediators with Gluconobacter oxydans Immobilized Whole Cells and Membrane Fractions in Oxidation of Ethanol
- 2012
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Ingår i: Electroanalysis. - : Wiley. - 1040-0397. ; 24:4, s. 924-930
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Tidskriftsartikel (refereegranskat)abstract
- Gluconobacter oxydans whole cells and membrane fractions in combination with ferrocene mediators were used to study oxidation of ethanol. The efficiency of mediator?enzyme interaction was assessed by the ratio of maximum current to the apparent Michaelis constant (Imax/KM) for saturating mediator concentrations. The bioelectrocatalytic processes were found to be more efficient with membrane fractions. The highest Imax/KM value of 120 and 3200 mu A g/mol for, respectively, cells and fractions was obtained for ferrocene carboxylic acid. In test measurements of biological oxygen demand for rye distillers grains, the values obtained by a biosensor based on Gluconobacter membrane fractions and ferrocene were found to correlate with the reference data.
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| 3. |
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| 4. |
- Plekhanova, Yu V., et al.
(författare)
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Biosensor Monitoring of Microbial Treatment of Wastewater from Nonylphenol Polyethoxylates under Flow-through Conditions
- 2011
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Ingår i: Applied Biochemistry and Microbiology. - 0003-6838. ; 47:9, s. 846-851
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Tidskriftsartikel (refereegranskat)abstract
- Biodegradation of nonylphenol polyethoxylates (NPEO) has been studied in laboratory column bioreactors with a polyethylene carrier on which destructor bacteria of Pseudomonas surface-active compounds were immobilized. To monitor the efficiency of microbial treatment of model wastewater, a biosensor based on the oxygen Clark electrode and destructor bacteria of NPEO from bioreactors was designed. The designed biosensor allowed us to detect the content of polyethylene glycol monoalkyl phenyl ether on the basis of polymer distillate (OP-10), which is a commercial NPEO preparation, in samples in the range of 1-200 mg/l. Operation of the biosensor was stable within 7 days with the standard deviation of 1.35 mg/l for 20 consecutive measurements of OP-10 concentration, which was 20 mg/l (7%).
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