| 1. |
- Beal, Jacob, et al.
(författare)
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Robust estimation of bacterial cell count from optical density
- 2020
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Ingår i: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
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Tidskriftsartikel (refereegranskat)abstract
- Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
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| 2. |
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| 3. |
- Altimira, Mireia, 1981-, et al.
(författare)
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Characterization of fan spray atomizers through numerical simulation
- 2009
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Ingår i: International Journal of Heat and Fluid Flow. - : Elsevier. - 0142-727X .- 1879-2278. ; 30:2, s. 339-355
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Tidskriftsartikel (refereegranskat)abstract
- The present paper focuses on the mathematical modeling of industrial fan spray atomizers. The two-phase flow taking place inside the nozzle's tip and the exterior region near the outlet of three different industrial nozzle designs has been modeled and simulated. As a result, valuable information has been obtained regarding the influence of the inner geometry on the flow and also the formation and development of the liquid sheet. Characteristic magnitudes such as the discharge coefficient and the liquid sheet thickness factor have been obtained and validated through experimental measurements. The accumulation of liquid at the border of fan-shaped liquid sheets, also known as rim, has been studied in the analyzed designs, revealing the presence of a tangential velocity component in the liquid sheet and a relationship between the incoming flow rate of the rim and the angle of the liquid sheet. The dependence of the results on turbulence modeling has also been analyzed, drawing interesting conclusions regarding their influence on the liquid sheet mean flow characteristics and on the surrounding gas. Thus, the mathematical model developed has been proved to be a useful tool for nozzle manufacturers; it provides the most important characteristic parameters of the liquid sheet formed given certain nozzle geometry and, additionally, those data necessary to carry out studies of instability, breakup and atomization of the liquid sheet.
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| 4. |
- Altimira, Mireia, 1981-, et al.
(författare)
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Disintegration regime of industrial fan-spray atomizers through CFD simulations
- 2011
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Ingår i: ILASS – Europe 2011, 24th European Conference on Liquid Atomization and Spray Systems, Estoril, Portugal, September 2011.
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Konferensbidrag (refereegranskat)abstract
- Among all the literature devoted to the investigation of sprays, very few works deal with the influence of the nozzle’s geometry on the characteristics of the spray produced, even though it has been proved to play a crucial role in certain operating regimes. The present paper presents criteria for the determination of the dominant disintegration regime in industrial fan-spray atomizers through CFD simulations accounting for the atomizer’s geometry.
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| 5. |
- Altimira, Mireia, 1981-, et al.
(författare)
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Fan-Spray Atomizers Analysis through Mathematical Modeling
- 2007
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Ingår i: Proceedings of the 21st ILASS - Europe Meeting 2007.
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Konferensbidrag (refereegranskat)abstract
- The main objective of this work is to determine the most important design parameters in fan spray atomizers and their effect in the atomization features in order to provide a design tool for nozzle manufacturers. A mathematical model relating the geometry and operating conditions to the characteristics of the spray has been obtained and validated. Three different sub-models have been used in series, so that the results of one model become inputs of the next one, namely multiphase flow simulation, instability and break-up model and maximum entropy model. These have been joined achieving a global model of the whole primary atomization process.
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| 6. |
- Altimira, Mireia, 1981-, et al.
(författare)
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Linear spatial instability of viscous flow of a liquid sheet through gas
- 2010
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Ingår i: Physics of fluids. - : American Institute of Physics (AIP). - 1070-6631 .- 1089-7666. ; 22:7
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Tidskriftsartikel (refereegranskat)abstract
- The present paper focuses on the linear spatial instability of a viscous two-dimensional liquid sheet bounded by two identical viscous gas streams. The Orr–Sommerfeld differential equations and the boundary conditions of the flow configuration are numerically solved using Chebyshev series expansions and the collocation method. The strong dependence of the instability parameters on the velocity profiles is proven by using both quadratic and error functions to define the base flow in the liquid sheet and the gas shear layer. The sensitivity of the spatial instability growth rate to changes in the dimensionless parameters of the problem is assessed. Regarding the liquid sheet Reynolds number, it has been observed that, when this parameter increases, both the most unstable growth rate and the corresponding wavenumber decrease, whereas the cutoff wavenumber increases. The results of this analysis are compared with temporal theory through Gaster transformation. The effects liquid and gas viscosity have on instability are studied by comparing the instability curves given by the presented model with those from an inviscid liquid sheet and a viscous liquid sheet in an inviscid gaseous medium. The model presented in this paper features a variation in the cutoff wavenumber with all the governing parameters of the problem, whereas that provided by cases that account for an inviscid surrounding gas depends only on the liquid sheet Weber number and the ratio of gas to liquid densities. Results provided by the presented model have been experimentally validated and show that quadratic profiles have a greater capacity to predict the disturbance wavelength.
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| 7. |
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| 8. |
- Benítez, G. E. F., et al.
(författare)
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Smartphone application for quantitative measurement of Parkinson tremors
- 2015
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Ingår i: IFMBE Proceedings. - Cham : Springer. - 9783319131160 ; , s. 785-788
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Konferensbidrag (refereegranskat)abstract
- One of the most common concerns in the atten-tion of patients with Parkinson's disease is an objectively eval-uation of the illness progress and the efficacy of treatments, in terms of the intensity and frequency of tremors. This symptom is produced by gradual degradation of the pigmented neurons located at the substantia nigra in the brain, in order to detect such movement levels. This paper proposes a Smartphone application for a quantitative detection, measurement and analysis of the Parkinson's tremor, due to the global use of Smartphones and the affordable cost of some Android plat-form devices. The subjects of this working project are those people who suffer from Parkinson's disease, who could down-load the application to their mobile phone in order to measure quantitatively the intensity and duration of their tremors, in any place, to send reports by email or to record them for a later use. The application enables the remote monitoring of the patients.
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| 9. |
- Bentham, James, et al.
(författare)
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A century of trends in adult human height
- 2016
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Ingår i: eLIFE. - 2050-084X. ; 5
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Tidskriftsartikel (refereegranskat)abstract
- Being taller is associated with enhanced longevity, and higher education and earnings. We reanalysed 1472 population-based studies, with measurement of height on more than 18.6 million participants to estimate mean height for people born between 1896 and 1996 in 200 countries. The largest gain in adult height over the past century has occurred in South Korean women and Iranian men, who became 20.2 cm (95% credible interval 17.522.7) and 16.5 cm (13.319.7) taller, respectively. In contrast, there was little change in adult height in some sub-Saharan African countries and in South Asia over the century of analysis. The tallest people over these 100 years are men born in the Netherlands in the last quarter of 20th century, whose average heights surpassed 182.5 cm, and the shortest were women born in Guatemala in 1896 (140.3 cm; 135.8144.8). The height differential between the tallest and shortest populations was 19-20 cm a century ago, and has remained the same for women and increased for men a century later despite substantial changes in the ranking of countries.
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| 10. |
- Bentham, James, et al.
(författare)
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A century of trends in adult human height
- 2016
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Ingår i: eLIFE. - : eLife Sciences Publications Ltd. - 2050-084X. ; 5
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Tidskriftsartikel (refereegranskat)abstract
- Being taller is associated with enhanced longevity, and higher education and earnings. We reanalysed 1472 population-based studies, with measurement of height on more than 18.6 million participants to estimate mean height for people born between 1896 and 1996 in 200 countries. The largest gain in adult height over the past century has occurred in South Korean women and Iranian men, who became 20.2 cm (95% credible interval 17.5–22.7) and 16.5 cm (13.3– 19.7) taller, respectively. In contrast, there was little change in adult height in some sub-Saharan African countries and in South Asia over the century of analysis. The tallest people over these 100 years are men born in the Netherlands in the last quarter of 20th century, whose average heights surpassed 182.5 cm, and the shortest were women born in Guatemala in 1896 (140.3 cm; 135.8– 144.8). The height differential between the tallest and shortest populations was 19-20 cm a century ago, and has remained the same for women and increased for men a century later despite substantial changes in the ranking of countries.
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| 11. |
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| 12. |
- Householder, John Ethan, et al.
(författare)
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One sixth of Amazonian tree diversity is dependent on river floodplains
- 2024
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Ingår i: NATURE ECOLOGY & EVOLUTION. - 2397-334X.
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Tidskriftsartikel (refereegranskat)abstract
- Amazonia's floodplain system is the largest and most biodiverse on Earth. Although forests are crucial to the ecological integrity of floodplains, our understanding of their species composition and how this may differ from surrounding forest types is still far too limited, particularly as changing inundation regimes begin to reshape floodplain tree communities and the critical ecosystem functions they underpin. Here we address this gap by taking a spatially explicit look at Amazonia-wide patterns of tree-species turnover and ecological specialization of the region's floodplain forests. We show that the majority of Amazonian tree species can inhabit floodplains, and about a sixth of Amazonian tree diversity is ecologically specialized on floodplains. The degree of specialization in floodplain communities is driven by regional flood patterns, with the most compositionally differentiated floodplain forests located centrally within the fluvial network and contingent on the most extraordinary flood magnitudes regionally. Our results provide a spatially explicit view of ecological specialization of floodplain forest communities and expose the need for whole-basin hydrological integrity to protect the Amazon's tree diversity and its function.
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| 13. |
- Larraona, Gorka S., et al.
(författare)
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Computational parametric study of an impinging jet in a cross-flow configuration for electronics cooling applications
- 2013
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Ingår i: Applied Thermal Engineering. - : Elsevier. - 1359-4311 .- 1873-5606. ; 52:2, s. 428-438
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Tidskriftsartikel (refereegranskat)abstract
- A parametric study based on design of experiments (DoE) techniques was carried out by computational simulation in order to evaluate the effect that design parameters have on heat transfer and pressure loss of an impinging jet in a cross-flow configuration. The main effects of each parameter and the interactions between parameters were analyzed in detail through the Response Surface Methodology (RSM). Additionally, the potential of the impinging jet in a cross-flow configuration was assessed by calculating the optimal values of the parameters and comparing the cooling efficiency of the resulting configuration with the efficiency of the conventional cross-flow configuration. It was found that the degree to which the average heat transfer coefficient is enhanced as the result of adding an impinging jet depends on the height of the cooled component. Specifically, it was found that the higher the component, the more significant the enhancement.
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| 14. |
- López-Isac, Elena, et al.
(författare)
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Brief Report : IRF4 Newly Identified as a Common Susceptibility Locus for Systemic Sclerosis and Rheumatoid Arthritis in a Cross-Disease Meta-Analysis of Genome-Wide Association Studies
- 2016
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Ingår i: Arthritis & Rheumatology. - : Wiley. - 2326-5191 .- 2326-5205. ; 68:9, s. 2338-2344
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Tidskriftsartikel (refereegranskat)abstract
- Objective: Systemic sclerosis (SSc) and rheumatoid arthritis (RA) are autoimmune diseases that have similar clinical and immunologic characteristics. To date, several shared SSc–RA genetic loci have been identified independently. The aim of the current study was to systematically search for new common SSc–RA loci through an interdisease meta–genome-wide association (meta-GWAS) strategy. Methods: The study was designed as a meta-analysis combining GWAS data sets of patients with SSc and patients with RA, using a strategy that allowed identification of loci with both same-direction and opposite-direction allelic effects. The top single-nucleotide polymorphisms were followed up in independent SSc and RA case–control cohorts. This allowed an increase in the sample size to a total of 8,830 patients with SSc, 16,870 patients with RA, and 43,393 healthy controls. Results: This cross-disease meta-analysis of the GWAS data sets identified several loci with nominal association signals (P < 5 × 10−6) that also showed evidence of association in the disease-specific GWAS scans. These loci included several genomic regions not previously reported as shared loci, as well as several risk factors that were previously found to be associated with both diseases. Follow-up analyses of the putatively new SSc–RA loci identified IRF4 as a shared risk factor for these 2 diseases (Pcombined = 3.29 × 10−12). Analysis of the biologic relevance of the known SSc–RA shared loci identified the type I interferon and interleukin-12 signaling pathways as the main common etiologic factors. Conclusion: This study identified a novel shared locus, IRF4, for the risk of SSc and RA, and highlighted the usefulness of a cross-disease GWAS meta-analysis strategy in the identification of common risk loci.
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| 15. |
- Luize, Bruno Garcia, et al.
(författare)
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Geography and ecology shape the phylogenetic composition of Amazonian tree communities
- 2024
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Ingår i: JOURNAL OF BIOGEOGRAPHY. - 0305-0270 .- 1365-2699.
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Tidskriftsartikel (refereegranskat)abstract
- Aim: Amazonia hosts more tree species from numerous evolutionary lineages, both young and ancient, than any other biogeographic region. Previous studies have shown that tree lineages colonized multiple edaphic environments and dispersed widely across Amazonia, leading to a hypothesis, which we test, that lineages should not be strongly associated with either geographic regions or edaphic forest types. Location: Amazonia. Taxon: Angiosperms (Magnoliids; Monocots; Eudicots). Methods: Data for the abundance of 5082 tree species in 1989 plots were combined with a mega-phylogeny. We applied evolutionary ordination to assess how phylogenetic composition varies across Amazonia. We used variation partitioning and Moran's eigenvector maps (MEM) to test and quantify the separate and joint contributions of spatial and environmental variables to explain the phylogenetic composition of plots. We tested the indicator value of lineages for geographic regions and edaphic forest types and mapped associations onto the phylogeny. Results: In the terra firme and v & aacute;rzea forest types, the phylogenetic composition varies by geographic region, but the igap & oacute; and white-sand forest types retain a unique evolutionary signature regardless of region. Overall, we find that soil chemistry, climate and topography explain 24% of the variation in phylogenetic composition, with 79% of that variation being spatially structured (R-2 = 19% overall for combined spatial/environmental effects). The phylogenetic composition also shows substantial spatial patterns not related to the environmental variables we quantified (R-2 = 28%). A greater number of lineages were significant indicators of geographic regions than forest types. Main Conclusion: Numerous tree lineages, including some ancient ones (>66 Ma), show strong associations with geographic regions and edaphic forest types of Amazonia. This shows that specialization in specific edaphic environments has played a long-standing role in the evolutionary assembly of Amazonian forests. Furthermore, many lineages, even those that have dispersed across Amazonia, dominate within a specific region, likely because of phylogenetically conserved niches for environmental conditions that are prevalent within regions.
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| 16. |
- Masip, Yunesky, et al.
(författare)
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Experimental study of the turbulent flow around a single wall-mounted cube exposed to a cross-flow and an impinging jet
- 2012
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Ingår i: International Journal of Heat and Fluid Flow. - : Elsevier. - 0142-727X .- 1879-2278. ; 38, s. 50-71
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Tidskriftsartikel (refereegranskat)abstract
- The air flow around a cubic obstacle mounted on one wall of a rectangular channel was studied experimentally. The obstacle represents an electronic component and the channel the space between two parallel printed circuit boards (PCBs). The flow was produced by the combination of a channel stream and a jet which issued from a circular nozzle placed at the wall opposite from where the component is mounted. With this aim, a test rig was designed and built to carry out experiments with both the above mentioned configurations and other cooling arrangements. Planar Particle Image Velocimetry (PIV) was employed to measure the instantaneous flow velocity on several planes covering the space around the component. The mean velocity and the Reynolds stresses were obtained from averaging the instantaneous velocity, and the mean flow showed a complex pattern with different features such as recirculation bubbles, vortices, detachment and reattachment zones. The influence of two parameters, namely the channel Reynolds number and the jet-to-channel Reynolds number ratio, on these flow features was studied considering nine cases that combined three values of the channel Reynolds number (3410, 5752 and 8880) and three values of the ratio (0.5, 1.0 and 1.5). The results show that the Reynolds number ratio determines the drag produced on the jet and the deflection from its geometric axis due to the channel stream. In all the cases corresponding to the lowest value of the ratio, the jet was dragged and did not impact the component. This fact accounts for the non-existence of the Upper Horseshoe Vortex and changes in the flow characteristics at the region over the component.
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| 17. |
- Rivas, Alejandro, et al.
(författare)
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Analysis of Liquid-Gas Flow near a Fan-Spray Nozzle Outlet
- 2006
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Ingår i: Conference on Modelling Fluid Flow (CMFF’06).
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Konferensbidrag (refereegranskat)abstract
- This paper reports the results of mathematical modelling and simulation of two-phase flow near the outlet of six different industrial fan-spray atomizers under different operating conditions. The Finite Volume Method, implemented in the commercial code, Fluent V.6.2, has been used to solve the mathematical model numerically, whilst the Volume of Fluid (VOF) approach has been employed to track the interface between liquid and gas. Surface tension effects have been included using the Continuum Surface Force method (CSF). Results from simulation have been validated with experimental data and compared with those from previous works. Interesting conclusions about sheet thickness, velocity and vorticity of the flow have been obtained; some of them differ from hypotheses assumed in previous works. These conclusions may reflect the way in which break-up models are used to obtain drop size and break-up length.
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| 18. |
- ter Steege, Hans, et al.
(författare)
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Mapping density, diversity and species-richness of the Amazon tree flora
- 2023
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Ingår i: COMMUNICATIONS BIOLOGY. - 2399-3642. ; 6:1
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Tidskriftsartikel (refereegranskat)abstract
- Using 2.046 botanically-inventoried tree plots across the largest tropical forest on Earth, we mapped tree species-diversity and tree species-richness at 0.1-degree resolution, and investigated drivers for diversity and richness. Using only location, stratified by forest type, as predictor, our spatial model, to the best of our knowledge, provides the most accurate map of tree diversity in Amazonia to date, explaining approximately 70% of the tree diversity and species-richness. Large soil-forest combinations determine a significant percentage of the variation in tree species-richness and tree alpha-diversity in Amazonian forest-plots. We suggest that the size and fragmentation of these systems drive their large-scale diversity patterns and hence local diversity. A model not using location but cumulative water deficit, tree density, and temperature seasonality explains 47% of the tree species-richness in the terra-firme forest in Amazonia. Over large areas across Amazonia, residuals of this relationship are small and poorly spatially structured, suggesting that much of the residual variation may be local. The Guyana Shield area has consistently negative residuals, showing that this area has lower tree species-richness than expected by our models. We provide extensive plot meta-data, including tree density, tree alpha-diversity and tree species-richness results and gridded maps at 0.1-degree resolution. A study mapping the tree species richness in Amazonian forests shows that soil type exerts a strong effect on species richness, probably caused by the areas of these forest types. Cumulative water deficit, tree density and temperature seasonality affect species richness at a regional scale.
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