| 1. |
- Bonde, Torben A., et al.
(författare)
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Microbial biomass as a fraction of potentially mineralizable nitrogen in soils from long-term field experiments
- 1988
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Ingår i: Soil Biology and Biochemistry. - : Elsevier. - 0038-0717 .- 1879-3428. ; 20:4, s. 447-452
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Tidskriftsartikel (refereegranskat)abstract
- Aerobic long-term incubations (40-wk) were employed to measure the potentially mineralizable nitrogen (N0) in five 30-yr old cropping systems. The cropping systems consisted of: (1) bare fallow; (2) cropping with no additions; (3) cropping with 80 kg N ha-1 y-1 as Ca(NO3)2; (4) cropping with 80 kg N ha-1 yr-1 as Ca(NO3)2 plus 1800kg C ha-1 yr-1 as straw; and (5) cropping with 80 kg N ha-1 yr-1 plus 1800 kg C ha-1 yr-1 as farmyard manure. The amounts of N mineralized during the 40-wk incubations were between 93 and 168 μg g-1 (302-543 kg N ha-1 down to 25cm depth) with the lowest value for the fallow and the highest for the farmyard manure treatment. Microbial biomass-C and -N were measured on four occasions during the incubations. The biomass-C showed a rapid decrease to week 4 (to 36% of the initial mass), a slower decrease to week 9 (to 23% of initial mass) and a very slow decline to the final determination at the end of the incubation (to 8% of initial mass). The biomass-N displayed a similar pattern. Two related models were employed to describe the kinetics of N-mineralization during incubation: (1) a two-component first-order; and (2) a simplified special case of the two-component model. In all cases except the straw-amended soil, the simplified two-component model offered the best description of the curves of accumulated mineral-N. The available fraction, Na, of soil organic-N had mineralization rate constants similar to those for mineralization of microbial biomass.
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| 6. |
- Klemendtsson, Leif, et al.
(författare)
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Microbial nitrogen transformations in the root environment of barley
- 1987
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Ingår i: Soil Biology and Biochemistry. - : Elsevier. - 0038-0717 .- 1879-3428. ; 19:5, s. 551-558
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Tidskriftsartikel (refereegranskat)abstract
- To determine the influence of barley roots on microorganisms and N-transfonning processes in soil, numbers of nitrifiers and potential nitrification and denitrification rates were measured every week for 5 wks. The barley plants were grown in growth chambers in which the root-containing soil layer (A) was separated from three outer soil layers (B, C, D). The numbers and biomass of bacteria, numbers of flagellates and amoebae, total and FDA-active hyphal lengths, microbial biomass carbon and respiration were also determined.The numbers of ammonium oxidizers were positively correlated with root biomass but did not differ significantly between soil layers. Potential ammonium oxidation was stimulated in the root-layer, while potential nitrite oxidation was stimulated in the B- and C-layers.The denitrification activity (measured anaerobically in the presence of excess No- 3) was positively correlated with root biomass in the A-layer. Denitrification activity in the B-layer was positively correlated with the water content of the soil. When roots grew near the nets separating the root layer from the other layers, denitrification activity was stimulated in the next layer (B).We propose that nitrite oxidation in the root zone partly depends on the reduction of nitrate. This would explain why nitrite-oxidizer numbers were usually several orders of magnitude higher than ammonium-oxidizer numbers.Bacterial numbers decreased between wks 1 and 5. Increases in bacteria, naked amoebae and flagellates in all layers between wks 2 and 3 indicated that bacteria were produced until wk 3. There were no signs of bacterial production after wk 3.The total length of hyphae and the length of FDA-active hyphae were not significantly different between layers. However, both of these parameters, as well as total microbial biomass carbon and respiration, were consistently highest in the A-layer.
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| 7. |
- Robertson, Kerstin, et al.
(författare)
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Microbial biomass in relation to C and N mineralization during laboratory incubations
- 1988
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Ingår i: Soil Biology and Biochemistry. - : Elsevier. - 0038-0717 .- 1879-3428. ; 20:3, s. 281-286
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Tidskriftsartikel (refereegranskat)abstract
- Net carbon and nitrogen mineralization rates were determined for an arable soil during 12 weeks at 37†C using an aerobic incubation-leaching technique. The amounts of mineralized C and N were compared to changes in the contents of C and N in microbial biomass (as determined by the chloroform fumigation incubation method; CFIM) during the incubation and to amounts of organic C and N in the leachates. Microorganisms were also followed by direct counting of bacteria, measurements of total hyphal lengths and fluorescein diacetate (FDA)-active hyphae, and by most probable number determinations of protozoa (naked amoebae and flagellates).Numbers of naked amoebae increased nearly 10-fold initially and then decreased between weeks 6 and 12. Bacterial numbers and FDA-active hyphae decreased during the incubation, and the relative composition changed slightly in favour of bacteria. Total hyphal lengths remained almost constant.A total of 105 μg N g'- soil dry wt and 1179 μg C g- soil dry wt was mineralized during the incubation, while the microbial N pool decreased by 42 γm- soil dry wt and the microbial C pool decreased by 225μ g- soil dry wt. Soluble organic matter in the leachates amounted to 16 and 31% of mineralized C and N, respectively.The possibility of measuring C mineralization with less frequent teachings and determinations of N mineralization offers an easy method for assessing changes in labile soil organic matter over time or for comparisons between soils. Through the use of appropriate C-to-N ratios, the N-content in the labile pool can be calculated.
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| 9. |
- Rosswall, Thomas
(författare)
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Introduction
- 2005
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Rapport (övrigt vetenskapligt/konstnärligt)abstract
- The presentation is only available as a sound file.
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| 10. |
- Schnürer, Johan, 1957-, et al.
(författare)
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Effects of moisture on soil microorganisms and nematodes : A field experiment
- 1986
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Ingår i: Microbial Ecology. - : Springer-Verlag New York. - 0095-3628 .- 1432-184X. ; 12:2, s. 217-230
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Tidskriftsartikel (refereegranskat)abstract
- The effects of soil moisture changes on bacteria, fungi, protozoa, and nematodes and changes in oxygen consumption were studied in a field experiment. In one plot the soil was drip-irrigated daily for 10 days, while an adjacent plot experienced one rainfall and was then allowed to dry out. Oxygen consumption was the parameter measured which responded most rapidly to changes in soil moisture content. Lengths of fluorescein diacetate-active hyphae paralleled oxygen consumption in both plots. Total hyphal length was not affected by one rainfall but increased from 700 mg-1 dry weight soil to more than 1,600 m in less than 10 days in the irrigated plot. In the rain plot, bacterial numbers doubled within 3 days and declined during the following period of drought. In the irrigated plot, numbers increased by 50% and then remained constant over the duration of the study. Only small changes in protozoan numbers were observed, with the exception of the last sampling date in the irrigated plot when large numbers of naked amoebae were recorded 2 days after a large natural rainfall. Nematode numbers, especially obligate root feeders, increased in both treatments. The increases were caused by decoiling rather than growth. The results indicate that fungal respiration was dominating, while bacteria, lacking a suitable source of energy, were less active, except for the first days.
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| 11. |
- Schnürer, Johan, 1957-, et al.
(författare)
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Fluorescein diacetate hydrolysis as a measure of total microbial activity in soil and litter
- 1982
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Ingår i: Applied and Environmental Microbiology. - Washington : American Society for Microbiology. - 0099-2240 .- 1098-5336. ; 43:6, s. 1256-1261
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Tidskriftsartikel (refereegranskat)abstract
- Spectrophotometric determination of the hydrolysis of fluorescein diacetate (FDA) was shown to be a simple, sensitive, and rapid method for determining microbial activity in soil and litter. FDA hydrolysis was studied in soil and straw incubated for up to 3h. Hydrolysis was found to increase linearly with soil addition. FDA hydrolysis by pure cultures of Fusarium culmorum increased linearly with mycelium addition both in shake cultures and after inoculation into sterile soil. FDA hydrolys is by Pseudomonas denitrificans increased linearly with biomass addition. The FDA hydrolytic activities in soil samples from different layers of an agricultural soil were correlated with respiration. Acetone was found to be suitable for terminating the reaction.
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| 12. |
- Schnürer, Johan, 1957-, et al.
(författare)
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Microbial biomass and activity in an agricultural soil with different organic matter contents
- 1985
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Ingår i: Soil Biology and Biochemistry. - : Elsevier. - 0038-0717 .- 1879-3428. ; 17:5, s. 611-618
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Tidskriftsartikel (refereegranskat)abstract
- Changes in soil fertility caused by various organic and N-fertilizer amendments were studied in a long-term field trial mostly cropped with cereals. Five treatments were included: (I) fallow, (II) cropping with no C or N addition, (III) cropping with N-fertilization (80 kg ha -1 yr-1), (IV) cropping with straw incorporation (1800kg Cha-1 yr-1) and N-fertilization (80 kg ha-1yr-1), and (V) cropping with addition of farmyard manure (80 kg N + 1800kg Cha-1yr-1). The treatments resulted in soil organic matter contents ranging from 4.3% (I) to 5.8% (V). Microbial biomass and activity were determined by chloroform fumigation, direct counting of fungi (fluorescein diacetate (FDA)-staining and Jones-Mollison agar-film technique) and bacteria (acridine orange staining), most probable number determinations of protozoa, esterase activity (total FDA hydrolysis) and respiration. Both biomass estimates and activity measurements showed a highly significant correlation with soil organic matter. Microbial biomass C ranged from 230 to 600 μg C g-1 dry wt soil, as determined by the fumigation technique, while conversions from direct counts gave a range from 380 to 2260 μg C. Mean hyphal diameters and mean bacterial cell volumes decreased with decreasing soil organic matter content.
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| 13. |
- Schnürer, Johan, 1957-, et al.
(författare)
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Mineralization of nitrogen from15N labelled fungi, soil microbial biomass and roots and its uptake by barley plants
- 1987
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Ingår i: Plant and Soil. - Dordrecht, Netherlands : Kluwer Academic Publishers. - 0032-079X .- 1573-5036. ; 102:1, s. 71-78
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Tidskriftsartikel (refereegranskat)abstract
- The availability of nitrogen in15N labelled fungi, soil microbial biomass (Ca(15NO3)2 immobilized by addition of glucose), barley roots and Ca(NO3)2 to barley plants was investigated in a greenhouse experiment. Samples of above-ground plant biomass were taken five times during 76 days. During this time, and at the start of the experiment, the C and N contents of the soil microbial biomass were determined. Microbial biomass-C decreased during the first 41 days, and then increased back to pre-treatment levels. Only 2% of the total soil15N was found in the microbial biomass two days after additions of Ca(15NO3)2. At the final sampling 76 days later, 17% of the15N remaining in soil was found in the microbial biomass. In the other tratments, microbial biomass-N accounted for 20% of remaining soil15N in the one that had received fungi, 29% in the one with barley roots and 35% in the Ca(NO3)2 plus glucose treatment. At harvest, 38% of the soil15N at day 0 added as Ca(NO3)2-N, 29% of fungal-N, 10% of N immobilized in the soil microbial biomass and 7% of N in barley roots was recovered in the above-ground plant biomass.It can be concluded that nitrogen in the native soil biomass is resistant to mineralization and plant uptake. The use of laboratory grown organisms for mineralization studies will overestimate the plant availability of nitrogen in soil microorganisms.
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