| 1. |
- Appelstrand, S. B., et al.
(författare)
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Patient-reported outcome measures in individuals with amelogenesis imperfecta: a systematic review
- 2022
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Ingår i: European Archives of Paediatric Dentistry. - : Springer Science and Business Media LLC. - 1818-6300 .- 1996-9805. ; 23:6, s. 885-895
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Tidskriftsartikel (refereegranskat)abstract
- Purpose Amelogenesis imperfecta (AI) is a hereditary condition which affects the composition and structure of enamel in terms of hypoplasia and/or hypomineralization. The condition severely affects patients facing such difficulties as hypersensibility, loss of tooth substance and poor aesthetics. The objective is to perform a systematic review of patient-reported outcome measures (PROMs) in patients with amelogenesis imperfecta. Methods Inclusion criteria were articles written in English, including PROMs from patients with amelogenesis imperfecta. The databases PubMed, Scopus and Web of Science were searched on April 27, 2022, and eligible articles were screened. Exclusion criteria were articles based on proxy reports and single case reports. Results 405 studies were screened in terms of title and abstract, with 31 articles eligible for full-text screening, resulting in a total of 11 articles eligible for inclusion, (articles including 4-82 patients). The content was analyzed, resulting in the outcome divided into seven domains: Oral Health-Related Quality of Life (OHRQoL), Dental fear, Esthetics, Psychosocial factors, Function, Dental hypersensitivity, and Treatment outcome. Conclusion The limited quantity of research on PROMS from patients with AI indicates a significant impact of OHRQoL and daily life. A large variety of approaches have been presented in the articles. Patients report concerns of esthetics, hypersensitivity, function, and a general impact on well-being and social interaction. This highlights the importance for the need of early dental treatment.
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| 2. |
- Davis, G. R., et al.
(författare)
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Microscopic study of dental hard tissues in primary teeth with Dentinogenesis Imperfecta Type II: Correlation of 3D imaging using X-ray microtomography and polarising microscopy
- 2015
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Ingår i: Archives of Oral Biology. - : Elsevier BV. - 0003-9969. ; 60:7, s. 1013-1020
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Tidskriftsartikel (refereegranskat)abstract
- Objectives: The aim of this study was to examine the histological appearance of dental hard tissues in primary teeth from children with DI using conventional polarised light microscopy and correlate that with 3D imaging using X-ray microtomograpy (XMT) to gain a further understanding of the dentine structure of teeth diagnosed with dentinogenesis imperfecta. Design: Undecalcified sections of primary teeth from patients diagnosed with Dentinogenesis Imperfecta Type II were examined using polarised light microscopy. XMT was employed for 3D-imaging and analysis of the dentine. Results: The polarised light microscopy and XMT revealed tubular structures in the dentine seen as vacuoles coinciding with the path of normal dentinal tubules but not continuous tubules. The size of the tubules was close to that of capillaries. The largest tubular structures had a direction corresponding to where the pulp tissue would have been located during primary dentine formation. The dysfunctional mineralisation of the dentine and obliteration of the pulp evidently leaves blood vessels in the dentine which have in the main been tied off and, in the undecalcified sections, appear as vacuoles. Conclusions: Although from radiographs, the pulp in teeth affected by Dentinogenesis Imperfect type II appears to be completely obliterated, a network of interconnected vessels may remain. The presence of large dentinal tubules and blood vessels, or the remnants of blood vessels, could provide a pathway for bacteria from the oral cavity. This might account for why some of these teeth develop periapical abscesses in spite of apparently having no pulp. (c) 2015 Elsevier Ltd. All rights reserved.
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| 3. |
- Hajdarevic, Adnan, 1994, et al.
(författare)
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Treatment choice for first permanent molars affected with molar-incisor hypomineralization, in patients 7-8 years of age: a questionnaire study among Swedish general dentists, orthodontists, and pediatric dentists
- 2024
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Ingår i: EUROPEAN ARCHIVES OF PAEDIATRIC DENTISTRY. - 1818-6300 .- 1996-9805.
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Tidskriftsartikel (refereegranskat)abstract
- PurposeThe aim of this study was to investigate attitudes and preferred therapy choice for first permanent molars (FPM) with Molar-Incisor Hypomineralization (MIH).MethodsAn online questionnaire was sent out to general dentists (n = 559) working in the Public Dental Service in Region Vastra Gotaland, orthodontists (n = 293), and pediatric dentists (n = 156) (members from each interest association), in Sweden. The questionnaire contained three parts: general questions regarding the respondents, patient cases, and general questions regarding extraction of FPMs with MIH. Statistics were carried out using Chi-squared tests, with a significance level of 5%.ResultsA response rate of 36% was obtained. Orthodontists and pediatric dentists were more prone to extract FPMs with both moderate and severe MIH, compared to general dentists. When restoring FPMs with moderate MIH, resin composite was preferred. Compared to the general dentists, the pediatric dentists were more prone to choose glass-ionomer cement in the FPMs with severe MIH. The most common treatment choice for FPMs with mild MIH was fluoride varnish. "When root furcation is under development of the second permanent molar on radiographs" was chosen as the optimal time for extracting FPMs with severe MIH, and the general dentists based their treatment decisions on recommendations from a pediatric dentist.ConclusionExtraction of FPMs with moderate and severe MIH is considered a therapy of choice among general dentists and specialists, and the preferred time of extraction is before the eruption of the second permanent molar.
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| 4. |
- Henriksen, J. S., et al.
(författare)
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A pilot study comparing optical coherence tomography, radiography, clinical photography, and polarisation microscopy for studies of hypomineralisation disturbances in enamel
- 2023
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Ingår i: Heliyon. - : Elsevier BV. - 2405-8440. ; 9:2
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Tidskriftsartikel (refereegranskat)abstract
- Aim: To investigate the use of optical coherence tomography (OCT) as a tool to assess general and localised hypomineralisation defects in the enamel. Design and Materials: Ten extracted permanent teeth (four teeth with localised hypomineralisa-tion, four teeth with general hypomineralisation, and two healthy controls) were used in this study. In addition, four participants who underwent OCT served as living controls for the extracted teeth.Methods: The OCT results were compared with clinical photographs, digital radiographs, and polarising microscopy images of tooth sections (considered the gold standard) to determine the method with the most accurate information regarding the extent of enamel disturbances: 1) visibility of enamel disturbance (visible yes/no); if yes, 2) extent of the disturbance in the enamel; and 3) determination of the plausible involvement of the underlying dentin.Results: OCT was more accurate than digital radiography and visual assessment. OCT could provide information about the extent of localised hypomineralised disturbances in the enamel that was comparable to that with polarisation microscopy of the tooth sections.Conclusion: Within the limitations of this pilot study, it can be concluded that OCT is suitable for investigating and evaluating localised hypomineralisation disturbances; however, it is less useful in cases with generalised hypomineralisation of the enamel. In addition, OCT complements radiographic examination of enamel; however, more studies are necessary to elucidate the full extent of the use of OCT in case of hypomineralisation.
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| 5. |
- Hultquist, A I, et al.
(författare)
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Integrating oral and social factors in individual caries risk assessments in preschool children-a registry-based study.
- 2024
-
Ingår i: European archives of paediatric dentistry : official journal of the European Academy of Paediatric Dentistry. - 1996-9805.
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Tidskriftsartikel (refereegranskat)abstract
- To investigate the predictive ability of individual Caries Risk Assessments (CRA) regarding oral factors supplemented with social factors in relation to caries outcome in preschool children. Furthermore, to assess various models of CRA with oral and social factors included, aiming to identify the most suitable models for different age groups.The design is a retrospective registry-based cohort study. Children visiting the dentists at ages 3 and 6years were included. Data on oral and social factors were obtained from dental records, the Swedish Quality register for caries and periodontitis (SKaPa), and Statistics Sweden (SCB). Various models of CRA were designed, combining oral and social factors. Models were analyzed with univariable associations using simple logistic regression, and the results were presented as odds ratios (ORs). In addition, models were analyzed with area under the receiver operating characteristic (ROC) curve (AUC). Pairwise comparisons were conducted by DeLong's test, with p<0.05 considered significant.Oral factors were the most significant for caries outcome (OR 9.6), followed by social factors: foreign background (OR 4.6), low income (OR 2.83), low education of the mother (OR 2.77), single-parent family (OR 2.11), and having≥3 siblings (OR 1.71), (p<0.01). The predictive ability of CRA improved when models combining oral and social factors were used, compaired to CRA based solely on oral factors (p<0.05). An increase of up to 15% was seen when CRA was conducted closer to the outcome.Models for Caries Risk Assessment including oral and social factors increase the predictive ability. Caries Risk Assessment has limited durability.
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| 6. |
- Jälevik, Birgitta, et al.
(författare)
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Can molar incisor hypomineralization cause dental fear and anxiety or influence the oral health-related quality of life in children and adolescents?-a systematic review
- 2022
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Ingår i: European Archives of Paediatric Dentistry. - : Springer Science and Business Media LLC. - 1818-6300 .- 1996-9805. ; 23, s. 65-78
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Tidskriftsartikel (refereegranskat)abstract
- Purpose Molar Incisor Hypomineralization (MIH) are first molars with developmental enamel defects and are common findings in many child populations. The porous nature of MIH enamel and the presence of post-eruptive enamel breakdown leads to the presence of hypersensitivity and pain, which is often the patient's main complaint and can result in dental fear and affect the quality of life. The present review aims to summarise the evidence for the ability of MIH to cause problems, such as dental fear and anxiety (DFA) and to summarise the evidence for a possibly negative impact on the oral health-related quality of life (OHRQoL) of MIH affected children and adolescents, in a systematic review. Method Two searches, (1) MIH AND dental anxiety and (2) MIH AND Quality of life, were performed in MEDLINE/PubMed and Scopus. Selection demands were fulfilling the MIH diagnosis criteria using validated instruments and questionnaires for assessing DFA and OHRQoL, respectively. Results After removing duplicates and articles not fulfilling the selection demands, 6 studies concerning MIH and DFA and 8 studies concerning MIH and OHRQoL remained. Conclusion Children and adolescents with diagnosed MIH did not seem to suffer from increased dental fear and anxiety, but indicated an impaired oral health-related quality of life.
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| 7. |
- Krekmanova, Larisa, 1959, et al.
(författare)
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Everyday- and dental-pain experiences in healthy Swedish 8-19 year olds: an epidemiological study.
- 2009
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Ingår i: International journal of paediatric dentistry / the British Paedodontic Society [and] the International Association of Dentistry for Children. - 1365-263X. ; 19:6, s. 438-47
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Tidskriftsartikel (refereegranskat)abstract
- BACKGROUND: Very little is known about children's everyday pains and dental treatment pains. A child's gender, age, and level of dental anxiety are factors that could interplay with the perception of pain and are thus worth studying. AIM: The objectives of this study were to investigate the frequency and reported intensity levels of children's everyday- and dental-pain experiences, and to study the reported pains in relation to gender, age, and dental anxiety. DESIGN: Three hundred and sixty-eight consecutive patients (8-19 years, mean age 13.5 years) from three different Public Dental Service were recruited. Pain ratings were obtained using McGrath's Children's Pain Inventory list and some additional items. Dental anxiety was estimated by the Dental Anxiety Scale. RESULTS: Most frequently experienced everyday pains were headache and tummy/stomach ache. Among dental treatment events, dental injection was reported to be most often ranked as painful, and more frequently by girls. Both dental and everyday pains were rated higher grouping children with high dental anxiety. CONCLUSIONS: The frequency of pain experiences are the same in Swedish children as in other populations. There is a relation between dental anxiety and the perception of pain.
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| 8. |
- Krekmanova, Larisa, 1959, et al.
(författare)
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Young patients' self-reported fear compared to professionals' assessments during invasive and non-invasive dental visits: a prospective, longitudinal study
- 2022
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Ingår i: European Archives of Paediatric Dentistry. - : Springer Science and Business Media LLC. - 1818-6300 .- 1996-9805. ; 23:2, s. 309-315
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Tidskriftsartikel (refereegranskat)abstract
- Purpose The main purpose was to study young patients' self-reports on dental fear over a 5-year period, prospectively. Also, to compare these to professionals' proxy reports for dental fear during invasive and non-invasive dental visits. The research question was, to what extent the self-reports and dental professionals' proxy reports are congruent, and if there were patient age-dependent differences. Methods 3134 patients from 11 public dental clinics, representing urban and rural areas, were invited. Four age cohorts were formed: 3, 7, 11, and 15 years of age and followed between the years 2008 and 2012. Dental examinations (non-invasive) and restorative treatments + extractions (invasive) were registered. During the treatments, self-reports regarding fear and professional proxy reports were registered: Not afraid at all = 0, little nervous = 1, quite afraid = 2, very scared = 3, terrified = 4. Results 2363 patients completed the cohort periods (51% girls and 49% boys). In all, 9708 dental examinations, restorations and extractions were performed. The fear prevalence increased with the invasiveness of the dental procedure; 7-56%. For dental examinations and restorations, fear declined with ascending age. The highest fear prevalence was reported for dental extractions. Younger children reported fear more frequently than older children, p < 0.001. Frequent inconsistencies between self-reports and proxy reports were observed among the younger children (16%) compared to the older children (8%), p < 0.001. Conclusion Non-congruence was observed for self-reports and proxy reports regarding all age cohorts.
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| 9. |
- McFarlane, G., et al.
(författare)
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Enamel daily secretion rates of deciduous molars from a global sample of children
- 2021
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Ingår i: Archives of Oral Biology. - : Elsevier BV. - 0003-9969. ; 132
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Tidskriftsartikel (refereegranskat)abstract
- Objective: To investigate and describe the variation in enamel daily secretion rates (DSRs) of naturally exfoliated deciduous molars (n = 345) from five modern-day populations (Aotearoa New Zealand, Britain, Canada, France, and Sweden). Design: Each tooth was thin sectioned and examined using a high-powered Olympus BX51 microscope and DP25 digital microscope camera. Mean DSRs were recorded for the inner, mid, and outer regions of cuspal and lateral enamel, excluding enamel nearest the enamel-dentin junction and at the outermost crown surface. Results: Mean DSRs did not vary significantly between populations, or by sex. Cuspal enamel grew slightly faster than lateral enamel (mean difference 0.16 mu m per day; p < 0.001). The trajectory of DSRs remained relatively constant from inner to outer cuspal enamel and increased slightly in lateral enamel (p = 0.003). Conclusions: The DSRs of deciduous molars from modern-day children are remarkably consistent when compared among populations. While growth rates are faster in cuspal than lateral enamel, the trajectory of enamel formation changes only slightly from inner to outer regions. The trajectory of DSRs for deciduous molars differs to that of permanent molar enamel, which typically display a steep increase in matrix deposition from inner to outer enamel.
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| 10. |
- Rexhaj, Flaureta, 1994, et al.
(författare)
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Evaluation of method parameters for sound undecalcified dental enamel proteomics using liquid chromatography-mass spectrometry
- 2023
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Ingår i: Archives of Oral Biology. - 0003-9969. ; 155
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Tidskriftsartikel (refereegranskat)abstract
- Objective: This study aims to validate a methodology for analyzing undecalcified, sound dental enamel proteomics using Liquid Chromatography-Mass Spectrometry (LC-MS). The study evaluates various parameters, including the impact of dental root coverage on protein contamination, the efficacy of protease inhibitors during enamel sample preparation, repeatability of LC-MS measurements on dental enamel, and statistical analysis. The study also assesses the effectiveness of combined trypsin and semi-trypsin searches in Mascot for obtaining additional protein identification data. Design: Sound dental enamel was removed using a wet grinding technique, then digested with trypsin and labeled with TMT prior to LC-MS analysis. The resulting proteomes were matched against the Homo sapiens Swissprot Database, with searches in Mascot performed using both trypsin and semitrypsin. Statistical methods were employed to analyze the data. Results: The study found that covering dental roots with composite during dental enamel microdissection is advisable, while using protease inhibition during microdissection may not be fully supported. The proteomic analyses demonstrated statistical repeatability and reliability, with consistent and reproducible proteomic data obtained from healthy dental enamel. Furthermore, employing both trypsin and semitrypsin searches in Mascot provided additional proteomic information. Conclusions: Overall, this study validates a methodology for analyzing undecalcified, sound dental enamel proteomics using LC-MS, and provides insights into various factors that can affect the quality and reliability of proteomic data. These findings have implications for future studies pursuant to understanding the proteomic mechanisms underlying dental enamel formation and other associated processes.
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| 11. |
- Rexhaj, Flaureta, 1994, et al.
(författare)
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Proteomic profiling of human dental enamel affected by molar incisor hypomineralisation of different clinical severity grades: an in vitro study
- 2024
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Ingår i: EUROPEAN ARCHIVES OF PAEDIATRIC DENTISTRY. - 1818-6300 .- 1996-9805.
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Tidskriftsartikel (refereegranskat)abstract
- Purpose The aim of this study was to explore the potential to profile and distinguish varying clinical severity grades of MIH, compared to normal enamel, using proteomics.Methods Liquid chromatography-mass spectrometry analyses were conducted on enamel samples of extracted teeth, from 11 children and adolescents, spanning an age range of 6-18 years. Enamel powder samples were collected from extracted, third molars (n = 3) and first permanent molars diagnosed with MIH (n = 8). The MIH tooth samples were categorized into subgroups based on clinical severity grade. The data were statistically analyzed using ANOVA and Welch's t test.Results Teeth affected by MIH exhibited a diverse array of proteins, each with different functions related to dental enamel, distinguishing them from their normal enamel counterparts. The application of microdissection combined with LC-MS techniques has revealed the potential to discern unique proteomic profiles among MIH-affected teeth, characterized by varying clinical severity grades. Both analyzed MIH groups displayed consistent trends in the presentation of biological processes, including underabundance of proteins primarily associated with cell organization and biogenesis. Furthermore, proteins linked to cell death were overabundant in both MIH groups.Conclusion Proteomics enabled the detection and differentiation of various proteins across different clinical severity grades of MIH.
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| 12. |
- Rythén, Marianne, et al.
(författare)
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Chemical aspects on dental hard tissues in primary teeth from preterm infants.
- 2010
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Ingår i: European journal of oral sciences. - : Wiley. - 1600-0722 .- 0909-8836. ; 118:4, s. 389-95
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Tidskriftsartikel (refereegranskat)abstract
- Preterm children with very low birth weight suffer from several neonatal and postnatal complications that may affect the mineralization of teeth. Clinical and morphological studies have shown enamel aberrations in teeth from preterm children. In this study, the chemical composition in enamel and dentin was compared in primary teeth from preterm children and full-term children, and the relationship between the chemical composition and the morphological appearance was investigated. Enamel and dentin in 17 exfoliated primary teeth, from 14 children with a gestational age below 29 wk, were investigated and compared with 36 exfoliated primary teeth from full-term children, using X-ray microanalyses (XRMA). In comparison with the teeth from the controls, the teeth from preterm children had a higher relative value of carbon (C), a lower relative value of calcium (Ca), a lower ratio of calcium/phosphorus (Ca/P) and a lower ratio of Ca/C throughout the outer part of the enamel. In dentin, the relative values for P were higher, and Ca/P ratio was lower, at the dentin-pulp junction. The Ca/P ratio indicated normal hydroxyapatite in the crystals in enamel and dentin. The lower ratio of Ca/C in the bulk and outer part of the enamel indicated more porous enamel.
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| 13. |
- Rythén, Marianne, et al.
(författare)
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Morphological aspects of dental hard tissues in primary teeth from preterm infants.
- 2008
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Ingår i: International Journal of Paediatric Dentistry. - 0960-7439 .- 1365-263X. ; 18:6, s. 397-406
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Tidskriftsartikel (refereegranskat)abstract
- BACKGROUND: Preterm children with very low birth weight suffer from several neonatal and post-natal complications that may affect the mineralization of the teeth. Clinical studies have shown enamel aberrations in both dentitions. AIMS: The aims of this study were to describe enamel histo-morphology in primary teeth, and investigate the relationship between medical history and morphological appearance. DESIGN: Dental enamels in 44 exfoliated primary teeth, from 14 children with a gestational age below 29 weeks and with a very low birth weight, were investigated, using polarized light microscopy (POLMI) and under a scanning electron microscope (SEM). RESULTS: The neonatal line was found in 1/3 of the sections located coronally of the crown. In the post-natal enamel, 31 teeth showed a degree of porosity higher than 5% with a varying extension. More than half of the teeth showed one or more increment lines. The SEM analysis confirmed the POLMI findings with irregular prisms covered with a structure-less film. CONCLUSIONS: Enamel from primary teeth of preterm children was found to have a high frequency of mineralization disturbances found in POLMI and SEM. The morphological features of the enamel from preterm children do not reflect the disturbances on general growth and development occurred during the neonatal period.
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| 14. |
- Sabel, Nina, 1973, et al.
(författare)
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Aesthetic assessment of maxillary lateral and canine implant-supported crowns by laypersons and orthodontists
- 2024
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Ingår i: Acta odontologica Scandinavica. - 1502-3850. ; 83, s. 362-370
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Tidskriftsartikel (refereegranskat)abstract
- INTRODUCTION: The objectives of this study were to evaluate how laypersons and orthodontists evaluate and rank aesthetic parameters of an implant-supported crown (ISC) on the canine position (ISC-C) and lateral position (ISC-L). METHODS: A digital survey of 11 cases, 5 ISC-C, 5 ISC-L and 1 control case without ISC, was distributed to 207 laypersons and 296 orthodontists. All cases included one extraoral photograph and three intraoral photographs. The respondents were asked to identify the ISC and to evaluate the aesthetic parameters regarding colour of the implant (CI), shape of the implant (SI) and gingival colour around ISC (GCI). Differences within and between the groups were tested using Chi-2-test and Independent-Samples t-test. RESULTS: All invited laypersons and 184 orthodontists (62% response rate) answered the survey. Orthodontists (89%) more correctly identified the ISC, regardless of its position, than laypersons (50%) (p < 0.001). Both laypersons (54%) and orthodontists (23%) rated higher proportions of acceptance of CI, SI and GCI in favour for the ISC-L than ISC-C (laypersons: 40%, orthodontists: 10%) (p < 0.001). Assessing each parameter separately, orthodontists rated higher for ISC-L, compared to the ISC-C (p < 0.001). In general, laypersons and orthodontist ranked tooth colour (mean, standard deviation [SD]:8.0,1.5 and 9.0, 1.0) and tooth shape (mean, SD: 8.0, 1.7 and 8.8, 1.2) as aesthetically higher than the gingival colour (mean, SD: 7.2, 2.2 and 8.0, 1.7) (p > 0.001). Conclusion: Laypersons and orthodontists consider the ISC-L as aesthetically more preferable, compared to the ISC-C.
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| 15. |
- Sabel, Nina, 1973, et al.
(författare)
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Analysis of some elements in primary enamel during postnatal mineralization.
- 2009
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Ingår i: Swedish dental journal. - 0347-9994. ; 33:2, s. 85-95
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Tidskriftsartikel (refereegranskat)abstract
- The primary teeth start to mineralize in utero and continue development and maturation during the first year of life.The aim of this study was to investigate the concentrations of some elements, C, F, Na, Mg, Cl, K and Sr, by secondary ion mass spectrometry (SIMS) in human primary incisors at different stages of mineralization.The teeth derived from an autopsy material from children who had died in sudden infant death.The buccal enamel of specimens from the ages 1, 2, 3, 4, 6 and 19 months, respectively, was analyzed. It was evident that posteruptive effects play an important role in composition of the outermost parts of the enamel. Before the tooth erupts, the concentrations of the elements vary with the maturation grade of the mineralization in the enamel. Sodium was the element with the highest concentration of the measured elements and chlorine was the element of lowest concentration.The 19 month old specimen, considered as the only mature and erupted tooth, showed to differ from the other specimens.The concentration of fluorine, in the 19 month old specimen's outermost surface, is readily seen higher compared with the other specimens at this depth zone. In the 19 month old specimen the concentration of carbon is lower. Potassium, sodium and chlorine have higher concentrations, in general, in the 19 month old specimen compared with the immature specimens. The thickness of the enamel during mineralization was calculated from data from SIMS.The thickness of the buccal enamel of primary incisors seemed to be fully developed between 3-4 months after birth, reaching a thickness of 350-400 microm.
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| 16. |
- Sabel, Nina, 1973, et al.
(författare)
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Demineralization of Enamel in Primary Second Molars Related to Properties of the Enamel
- 2012
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Ingår i: Scientific World Journal. - : Hindawi Limited. - 1537-744X.
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Tidskriftsartikel (refereegranskat)abstract
- Enamel structure is of importance in demineralization. Differences in porosity in enamel effect the rate of demineralization, seen between permanent and deciduous teeth. Individual differences have been shown in the mean mineral concentration values in enamel, the role of this in demineralization is not thoroughly investigated. The aim of this study was to study variations of depths of artificial lesions of demineralization and to analyze the depth in relation to variations in the chemical and mineral composition of the enamel. A demineralized lesion was created in second primary molars from 18 individuals. Depths of lesions were then related to individual chemical content of the enamel. Enamel responded to demineralization with different lesion depths and this was correlated to the chemical composition. The carbon content in sound enamel was shown to be higher where lesions developed deeper. The lesion was deeper when the degree of porosity of the enamel was higher.
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| 17. |
- Sabel, Nina, 1973, et al.
(författare)
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Dental caries and oral health-related quality of life in Preschoolers–introducing the Swedish version of the early childhood oral health impact scale (ECOHIS)
- 2024
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Ingår i: Acta Odontologica Scandinavica. - 0001-6357 .- 1502-3850. ; 83
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Tidskriftsartikel (refereegranskat)abstract
- Objective: Experience of caries has a clearly negative impact on the quality of life in preschool children. The instrument Early Childhood Oral Health Impact Scale (ECOHIS) measures the oral health-related quality of life in preschool children (Child Impact Section) and their families (Family Impact Section). The aims of the study were to develop a Swedish version of ECOHIS and to evaluate the instrument’s reliability, validity, and internal consistency. Additionally, to analyse the oral health-related quality of life (OHRQoL) among preschool children who have experienced caries. Methods: The original ECOHIS questionnaire was translated into Swedish. Caregivers of preschool children aged 2–5 years were recruited at dental clinics in Sweden, to participate in the study and answer the Swedish version of the ECOHIS (S-ECOHIS). The internal consistency and reliability were assessed by using Cronbach’s Alpha coefficient. In order to measure the consistency of the study, the questionnaire was re-tested two weeks later for 10 of the caregivers and assessed by using intra-class correlation coefficients (ICCs). The results from S-ECOHIS were described as descriptive data and independent t-test was performed. All data were calculated using SPSS (Statistical Package for the Social Sciences). Results: S-ECOHIS was developed by translating the original English version using a double-blinded technique. A total of 274 caregivers participated in the study and completed the questionnaire. Cronbach’s Alpha was 0.84 for S-ECOHIS, 0.83 for CIS, and 0.66 for FIS. The ICC was 0.95 for the test-retest of S-ECOHIS. Among the respondents, 117 (43%) had children diagnosed with caries, while 157 (57%) had children without caries. The children with caries reported a higher total score of 5.97 (SD 6.16) of S-ECOHIS, compared to the score of the non-caries children 0.77 (SD 1.38) (p < 0.001). Conclusion: The Swedish version of ECOHIS that was developed demonstrates good validity, test-retest reliability, and internal consistency. The findings show that the oral health-related quality of life is adversely affected in preschool children with caries, with particular vulnerability observed among children with untreated caries. These results indicate that S-ECOHIS is suitable for use in future clinical and research endeavors.
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| 18. |
- Sabel, Nina, 1973, et al.
(författare)
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Elemental composition of normal primary tooth enamel analyzed with XRMA and SIMS.
- 2009
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Ingår i: Swedish dental journal. - 0347-9994. ; 33:2, s. 75-83
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Tidskriftsartikel (refereegranskat)abstract
- There is an interest to analyze the chemical composition of enamel in teeth from patients with different developmental disorders or syndromes and evaluate possible differences compared to normal composition. For this purpose, it is essential to have reference material. The aim of this study was to, by means of X-ray micro analyses (XRMA) and secondary ion mass spectrometry (SIMS), present concentration gradients for C, O, P and Ca and F, Na, Mg, Cl, K and Sr in normal enamel of primary teeth from healthy individuals. 36 exfoliated primary teeth from 36 healthy children were collected, sectioned, and analyzed in the enamel and dentin with X-ray micro analyses for the content of C, O, P and Ca and F, Na MgCl, K and Sr. This study has supplied reference data for C, O, P and Ca in enamel in primary teeth from healthy subjects. No statistically significant differences in the elemental composition were found between incisors and molars.The ratio Ca/P is in concordance with other studies. Some elements have shown statistically significant differences between different levels of measurement. These results may be used as reference values for research on the chemical composition of enamel and dentin in primary teeth from patients with different conditions and/or syndromes.
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| 19. |
- Sabel, Nina, 1973
(författare)
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Enamel of primary teeth - morphological and chemical aspects
- 2012
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Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- Enamel is one of the most important structures of the tooth, both from a functional and esthetic point of view. Primary enamel carries registered information regarding metabolic and physiological events that occurred during the period around birth and the first year of life. Detailed knowledge of normal development and the structure of enamel is important for the assessment of mineralization defects. The aim of the thesis is to add more detailed information regarding the structure of primary enamel. The structural appearance of the neonatal line and the quantitative developmental enamel defect, enamel hypoplasia, was thoroughly investigated with a polarized light microscope, microradiography and scanning electron microscope. X-ray microanalysis of some elements was also performed across the enamel and the neonatal line. Postnatal mineralization of enamel at different ages and from different individuals was studied regarding the chemical content, by using secondary ion mass spectrometry. The enamel’s response to demineralization was investigated in relation to the individual chemical content and the degree of mineralization of the enamel, by using polarized light microscope, microradiography, scanning electron microscope and X-ray microanalysis. The neonatal line is a hypomineralized structure seen as a step-like rupture in the enamel matrix. The neonatal line is due to disturbances in the enamel secretion stage. The enamel prisms in the postnatal enamel appeared to be smaller than the prenatal prisms. The hypoplasias showed a rough surface at the base and no aprismatic surface layer was seen in the defect. The enamel of the rounded border of hypoplasia appeared to be hypomineralized, with the bent prisms not being densely packed. Mineralization of enamel is a gradual process, still continuous at 6 months postnatally in the primary mandibular incisors. The thickness of the buccal enamel is reached at 3-4 months of age. Demineralization of enamel depends on the degree of mineralization and the chemical content of the enamel exposed. In a more porous enamel, deeper lesions will develop. The posteruptive maturation has a beneficial effect on the enamel’s resistance to demineralization.
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| 20. |
- Sabel, Nina, 1973, et al.
(författare)
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Neonatal lines in the enamel of primary teeth--a morphological and scanning electron microscopic investigation.
- 2008
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Ingår i: Archives of oral biology. - : Elsevier BV. - 1879-1506 .- 0003-9969. ; 53:10, s. 954-63
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Tidskriftsartikel (refereegranskat)abstract
- OBJECTIVE: The neonatal line (NNL) is in principle found in all primary teeth and the line represents the time of birth. Earlier findings of the appearance of the NNL in light microscope and in microradiographs have shown not only changes in the prism direction of the enamel, but that the NNL has a hypomineralized character. METHODS: The neonatal line was analyzed in un-decalcified sections of primary lower and central incisors, collected from individuals of different ages utilizing polarized light microscopy, microradiography, scanning electron microscopy (SEM) and X-ray analysis (XRMA). RESULTS: In polarized light the NNL appeared to have a more porous structure than the enamel in general. The appearance of the NNL as a dark line in microradiographs is interpreted as the NNL being less mineralized than neighbouring enamel. Analysis with ImageJ visualized the reduction of the amount of grey value, indicating that the NNL is less mineralized. Analysis of the NNL in SEM showed a reduction of the diameter of enamel prisms, the more narrow diameters continued through the postnatal enamel. A change of the growth direction of the prisms was also observed at the NNL. In a three-dimensional image the NNL appeared as a grove, however, in non-etched enamel no grove was seen. The elemental analyses with XRMA showed no marked changes in the content of C, Ca, P, N, O or S in the area around the NNL. CONCLUSIONS: The NNL is an optical phenomenon due to alterations in height, and degree of mineralization of the enamel prisms.
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| 21. |
- Sabel, Nina, 1973, et al.
(författare)
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Polarized light and scanning electron microscopic investigation of enamel hypoplasia in primary teeth.
- 2010
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Ingår i: International journal of paediatric dentistry. - 1365-263X .- 0960-7439. ; 20:1, s. 31-36
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Tidskriftsartikel (refereegranskat)abstract
- BACKGROUND: Enamel hypoplasia is a developmental disturbance during enamel formation, defined as a macroscopic defect in the enamel, with a reduction of the enamel thickness with rounded, smooth borders. Information on the microstructural level is still limited, therefore further studies are of importance to better understand the mechanisms behind enamel hypoplasia. AIM: To study enamel hypoplasia in primary teeth by means of polarized light microscopy and scanning electron microscopy. METHODS: Nineteen primary teeth with enamel hypoplasia were examined in a polarized light microscope and in a scanning electron microscope. RESULTS: The cervical and incisal borders of the enamel hypoplasia had a rounded appearance, as the prisms in the rounded cervical area of the hypoplasia were bent. The rounded borders had a normal surface structure whereas the base of the defects appeared rough and porous. CONCLUSIONS: Morphological findings in this study indicate that the aetiological factor has a short duration and affects only certain ameloblasts. The bottom of the enamel hypoplasia is porous and constitutes possible pathways for bacteria into the dentin.
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| 22. |
- Sabel, Nina, 1973, et al.
(författare)
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X-ray microanalysis of dentine in primary teeth diagnosed Dentinogenesis Imperfecta type II
- 2020
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Ingår i: European Archives of Paediatric Dentistry. - : Springer Science and Business Media LLC. - 1818-6300 .- 1996-9805. ; 21, s. 527-535
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Tidskriftsartikel (refereegranskat)abstract
- Aim To analyse the elemental composition of dentine in primary teeth from children diagnosed with Dentinogenesis Imperfecta type II (DI) and from normal sound primary teeth using X-ray microanalysis. Materials and methods X-ray microanalysis of the elements C, O, Na, Mg, P, Cl, K and Ca were performed in the dentine of five normal primary teeth and in seven primary teeth diagnosed DI. The analysis was made in a low magnification in 10 points from the enamel-dentine junction/root surface toward the pulp. The data was also evaluated with an inductive analysis. Results Lower values for C were found in DI-dentine compared with normal dentine. Na had significantly higher values in DI-dentine while Mg had significantly lower values. The inductive analysis revealed that Na and Mg were the most important elements for discriminating DI-dentine from normal dentine. Conclusions Dentine in primary teeth from patients diagnosed with Dentinogenesis Imperfecta type II analysed with XRMA have lower values of C and Mg and higher values of O and Na compared with normal primary dentine.
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| 23. |
- Sabel, Nina, 1973, et al.
(författare)
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XRMA analysis and X-ray diffraction analysis of dental enamel from human permanent teeth exposed to hydrogen peroxide of varying pH
- 2019
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Ingår i: Journal of Clinical and Experimental Dentistry. - : Medicina Oral, S.L.. - 1989-5488. ; 11:6, s. e512-e520
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Tidskriftsartikel (refereegranskat)abstract
- Background : This in vitro investigation shows how 3.3% H2O2, at different pH-values affects the enamel. Material and Methods : A number of fifteen human premolars were used. The enamel of the coronal half in six of the teeth, were exposed by H2O2. Nine teeth were prepared to enamel powder. The enamel was exposed to 3.3% H2O2, at six different pH-values (pH range 4.5 - 7.0). Analyses of the topography of enamel performed by scanning electron microscope (SEM) and the chemical composition of enamel by X-ray microanalysis (XRMA). X-ray powder diffraction (XRD) analysed the crystallinity in enamel powder. Results : The exposure to H2O2 at pH < 5.5 resulted in a rougher topography of the enamel, according to the SEM studies. The XRMA analysis revealed a increase in the ratio of Ca:C. Exposure to H2O2 at pH > 5.5 resulted in a decrease of O in the exposed enamel, and changes in C:P, Ca:C, Ca:P and Ca:O were observed. The H2O22 did not affect the unit cell parameters, but the signal-to-noise level was increased for slightly acidic or neutral solutions. The unit cell parameters decreased in the acidic solutions. Conclusions : The exposure to H2O2 at varying pH values affect the enamel with two different mechanisms. One effect is the oxidation of the organic or bioorganic matter in the hydroxyapatite matrix, due to the use of 3.3% H2O2. The other effect is due to the current pH of the H2O2, since the structure of the hydroxyapatite starts to erode when the pH < 5.5.
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| 24. |
- Westerlund, Anna, et al.
(författare)
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Epigenetic markers of tooth eruption - DNA methylation and histone acetylation.
- 2024
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Ingår i: European Journal of Oral Sciences. - 0909-8836. ; 132:4
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Tidskriftsartikel (refereegranskat)abstract
- The present study aimed to evaluate whether epigenetic markers are expressed in the dental follicles surrounding ectopically erupting teeth. Twenty-one dental follicles were collected in 20 adolescent children through surgical exposure of ectopic teeth. The epigenetic modifications of DNA methylation and histone acetylation were evaluated by immunohistochemistry. The results showed cells positive for DNA-methyltransferase 1 (DNMT1), DNA methyltransferase 3 beta (DNMT3B), ten-eleven translocation-2 (TET2), acetyl-histone H3 (AcH3), acetyl-histone H4 (AcH4), 5-methylcytosine (5mC), and 5-hydroxymethylcytosine (5hmC) were present in all the samples. The levels of epigenetic markers representing active chromatin (5hmC, AcH3, AcH4, and TET2) were statistically significantly higher than those of markers representing inactive chromatin (5mC, DNMT3B, DNMT1). In conclusion, follicles in ectopic teeth display major epigenetic modifications. In the follicles, epigenetic markers associated with the activation of bone-related genes are more abundant than markers associated with the inactivation of bone-related genes.
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