| 1. |
- Sodergren, Erica, et al.
(författare)
-
The genome of the sea urchin Strongylocentrotus purpuratus.
- 2006
-
Ingår i: Science. - : American Association for the Advancement of Science (AAAS). - 1095-9203 .- 0036-8075. ; 314:5801, s. 941-52
-
Tidskriftsartikel (refereegranskat)abstract
- We report the sequence and analysis of the 814-megabase genome of the sea urchin Strongylocentrotus purpuratus, a model for developmental and systems biology. The sequencing strategy combined whole-genome shotgun and bacterial artificial chromosome (BAC) sequences. This use of BAC clones, aided by a pooling strategy, overcame difficulties associated with high heterozygosity of the genome. The genome encodes about 23,300 genes, including many previously thought to be vertebrate innovations or known only outside the deuterostomes. This echinoderm genome provides an evolutionary outgroup for the chordates and yields insights into the evolution of deuterostomes.
|
|
| 2. |
|
|
| 3. |
- Regev, A, et al.
(författare)
-
The Human Cell Atlas
- 2017
-
Ingår i: eLife. - : ELIFE SCIENCES PUBLICATIONS LTD. - 2050-084X. ; 6
-
Tidskriftsartikel (refereegranskat)
|
|
| 4. |
- Daniel, Michael G., et al.
(författare)
-
Induction of human hemogenesis in adult fibroblasts by defined factors and hematopoietic coculture
- 2019
-
Ingår i: FEBS Letters. - : Wiley. - 0014-5793 .- 1873-3468. ; 593:23, s. 3266-3287
-
Tidskriftsartikel (refereegranskat)abstract
- Transcription factor (TF)-based reprogramming of somatic tissues holds great promise for regenerative medicine. Previously, we demonstrated that the TFs GATA2, GFI1B, and FOS convert mouse and human fibroblasts to hemogenic endothelial-like precursors that generate hematopoietic stem progenitor (HSPC)-like cells over time. This conversion is lacking in robustness both in yield and biological function. Herein, we show that inclusion of GFI1 to the reprogramming cocktail significantly expands the HSPC-like population. AFT024 coculture imparts functional potential to these cells and allows quantification of stem cell frequency. Altogether, we demonstrate an improved human hemogenic induction protocol that could provide a valuable human in vitro model of hematopoiesis for disease modeling and a platform for cell-based therapeutics. Database: Gene expression data are available in the Gene Expression Omnibus (GEO) database under the accession number GSE130361.
|
|
| 5. |
- Divagar, M, et al.
(författare)
-
Self-assembled polyamidoamine dendrimer on poly (methyl methacrylate) for plasmonic fiber optic sensors
- 2019
-
Ingår i: ChemNanoMat. - : John Wiley & Sons. - 2199-692X.
-
Tidskriftsartikel (refereegranskat)abstract
- We report a novel one-step polyamidoamine (PAMAM) dendrimer based polymethyl methacrylate (PMMA) surface functionalization strategy for the development of polymeric optical fiber (POF) based plasmonic sensors utilizing gold nanoparticles (AuNP). Simple contact angle measurements over PMMA sheets reveal the ability of the dendrimers to strongly bind to PMMA surface without additional acid/alkali pretreatment, unlike the conventional hexamethylene diamine (HMDA) based surface modification. Subsequently, U-bent POF probes with high evanescent wave absorbance sensitivity were exploited for relative quantification of the surface amine groups using fluorescein isothiocyanate (FITC) binding and efficient chemisorption of gold nanoparticles (AuNP) in order to identify the optimum conditions viz. dendrimer concentration, incubation time and dendrimer generation. While FITC binding showed a proportional increase in amine functional density with PAMAM concentration and time, interestingly the AuNP (40 nm) binding studies revealed the formation of loose PAMAM multilayers and their desorption. PAMAM (G4) concentration as low as 5 mM and incubation time of 24 h provide faster binding rate with densely packed AuNP and the RI sensitivity of ~15 (A546 nm/RIU). This simpler and inexpensive strategy could also be exploited for the development of functional PMMA substrates for various applications including nanotechnology, bio-imaging, drug delivery and analytical separations.
|
|
| 6. |
- Gomes, Andreia M., et al.
(författare)
-
Cooperative Transcription Factor Induction Mediates Hemogenic Reprogramming
- 2018
-
Ingår i: Cell Reports. - : Elsevier BV. - 2211-1247.
-
Tidskriftsartikel (refereegranskat)abstract
- During development, hematopoietic stem and progenitor cells (HSPCs) arise from specialized endothelial cells by a process termed endothelial-to-hematopoietic transition (EHT). The genetic program driving human HSPC emergence remains largely unknown. We previously reported that the generation of hemogenic precursor cells from mouse fibroblasts recapitulates developmental hematopoiesis. Here, we demonstrate that human fibroblasts can be reprogrammed into hemogenic cells by the same transcription factors. Induced cells display dynamic EHT transcriptional programs, generate hematopoietic progeny, possess HSPC cell surface phenotype, and repopulate immunodeficient mice for 3 months. Mechanistically, GATA2 and GFI1B interact and co-occupy a cohort of targets. This cooperative binding is reflected by engagement of open enhancers and promoters, initiating silencing of fibroblast genes and activating the hemogenic program. However, GATA2 displays dominant and independent targeting activity during the early phases of reprogramming. These findings shed light on the processes controlling human HSC specification and support generation of reprogrammed HSCs for clinical applications. Gomes et al. show that specification of hemogenesis in human fibroblasts is mediated by cooperative transcription factor binding. GATA2 displays dominance, interacts with GFI1B, and recruits FOS to open chromatin, simultaneously silencing the fibroblast program and initiating an endothelial-to-hematopoietic transition to definitive hematopoiesis.
|
|
| 7. |
- Haniffa, Muzlifah, et al.
(författare)
-
A roadmap for the Human Developmental Cell Atlas
- 2021
-
Ingår i: Nature. - : Springer Science and Business Media LLC. - 0028-0836 .- 1476-4687. ; 597:7875, s. 196-205
-
Tidskriftsartikel (refereegranskat)abstract
- This Perspective outlines the Human Developmental Cell Atlas initiative, which uses state-of-the-art technologies to map and model human development across gestation, and discusses the early milestones that have been achieved. The Human Developmental Cell Atlas (HDCA) initiative, which is part of the Human Cell Atlas, aims to create a comprehensive reference map of cells during development. This will be critical to understanding normal organogenesis, the effect of mutations, environmental factors and infectious agents on human development, congenital and childhood disorders, and the cellular basis of ageing, cancer and regenerative medicine. Here we outline the HDCA initiative and the challenges of mapping and modelling human development using state-of-the-art technologies to create a reference atlas across gestation. Similar to the Human Genome Project, the HDCA will integrate the output from a growing community of scientists who are mapping human development into a unified atlas. We describe the early milestones that have been achieved and the use of human stem-cell-derived cultures, organoids and animal models to inform the HDCA, especially for prenatal tissues that are hard to acquire. Finally, we provide a roadmap towards a complete atlas of human development.
|
|
| 8. |
- Stålgren, Johan J. R., et al.
(författare)
-
Enrichment of deuterium oxide at hydrophilic interfaces in aqueous solutions
- 2007
-
Ingår i: Langmuir. - : American Chemical Society (ACS). - 0743-7463 .- 1520-5827. ; 23:24, s. 11943-11946
-
Tidskriftsartikel (refereegranskat)abstract
- The structure of water at aqueous interfaces is of the utmost importance in biology, chemistry, and geology. We use neutron reflectivity and quartz crystal microbalance to probe an interface between hydrophilic quartz and bulk liquid solutions of H2O/D2O mixtures. We find that near the interface the neutron scattering length density is larger than in the bulk solution and there is an excess adsorbed mass. We interpret this as showing that there is a region adjacent to the quartz that is enriched in D2O and extends 5-10 nm into the solution. This suggests caution when interpreting results where D2O is substituted for H2O in aqueous interfacial chemistry.
|
|
| 9. |
- Svanedal, Ida, 1979-, et al.
(författare)
-
Impact of the Amphoteric Nature of a Chelating Surfactant on its Interaction with an Anionic Surfactant : A Surface Tension and Neutron Reflectivity Study of Binary Mixed Solutions
- 2024
-
Ingår i: ACS Omega. - : American Chemical Society (ACS). - 2470-1343. ; 9:10, s. 11366-11376
-
Tidskriftsartikel (refereegranskat)abstract
- 2-Dodecyldiethylenetriaminepentaacetic acid (C12-DTPA) is a chelating, amphoteric surfactant with a bulky headgroup containing eight pH-responsive groups. The hypothesis was that the amphoteric nature of the chelating surfactant would affect the interaction with another surfactant and, consequently, also the composition of mixed surface layers. Binary mixed monolayers of C12-DTPA and the anionic surfactant sodium dodecyl sulfate (SDS) were examined using neutron reflection and surface tension measurements. The experiments were conducted at pH 5, where the C12-DTPA monomers carried a net negative charge. Surface excess calculations at low total surfactant concentration revealed that the chelating surfactant dominated the surface composition. However, as the concentration was raised, the surface composition shifted toward an SDS-dominant state. This phenomenon was attributed to the increased ionic strength at increased concentrations, which altered the balance between competing entropic forces in the system. Interaction parameters for mixed monolayer formation were calculated, following a framework based on regular solution theory. In accordance with the hypothesis, the chelating surfactant’s ability to modulate its charge and mitigate repulsive interactions in the surface layer resulted in favorable interactions between the anionic SDS and negatively charged C12-DTPA monomers. These interactions were found to be concentration-dependent, which was consistent with the observed shift in the surface layer composition.
|
|
