| 1. |
- Sharma, Yashpal, et al.
(författare)
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Fabrication of conducting electrospun nanofibers scaffold for three-dimensional cells culture
- 2012
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Ingår i: International Journal of Biological Macromolecules. - : Elsevier. - 0141-8130 .- 1879-0003. ; 51:4, s. 627-631
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Tidskriftsartikel (refereegranskat)abstract
- Electrospinning is a versatile method to fabricate nanofibers of a range of polymeric and composite materials suitable as scaffolds for tissue engineering applications. In this study, we report the fabrication and characterization of polyaniline-carbon nanotube/poly(N-isopropyl acrylamide-co-methacrylic acid) (PANI-CNT/PNIPAm-co-MAA) composite nanofibers and PNIPAm-co-MAA nanofibers suitable as a three-dimensional (3D) conducting smart tissue scaffold using electrospinning. The chemical structure of the resulting nanofibers was characterized with FUR and H-1 NMR spectroscopy. The surface morphology and average diameter of the nanofibers were observed by SEM. Cellular response of the nanofibers was studied with mice L929 fibroblasts. Cell viability was checked on 7th day of cell culture by double staining the cells with calcein-AM and PI dye. PANI-CNT/PNIPAm-co-MAA composite nanofibers were shown the highest cell growth and cell viability as compared to PNIPAm-co-MAA nanofibers. Cell viability in the composite nanofibers was obtained in order of 98% that indicates the composite nanofibers provide a better environment as a 3D scaffold for the cell proliferation and attachment suitable for tissue engineering applications.
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| 2. |
- Tiwari, Ashutosh, et al.
(författare)
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Influence of poly(N-isopropylacrylamide)-CNT-polyaniline three-dimensional electrospun microfabric scaffolds on cell growth and viability
- 2013
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Ingår i: Biopolymers. - : John Wiley & Sons. - 0006-3525 .- 1097-0282. ; 99:5, s. 334-341
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Tidskriftsartikel (refereegranskat)abstract
- This study investigates the effect on: 1) the bulk surface; and 2) the three-dimensional non-woven microfabric scaffolds of poly(N-isopropylacylamide)-CNT-polyaniline on growth and viability of mice fibroblast cells L929. The poly(N-isopropylacylamide)-CNT-polyaniline was prepared using coupling chemistry and electrospinning was then used for the fabrication of responsive, nonwoven microfabric scaffolds. The electrospun microfabrics were assembled in regular three-dimensional scaffolds with OD: 400-500 mm; L: 6-20 cm. Mice fibroblast cells L929 were seeded on the both poly(N-isopropylacylamide)-CNT-polyaniline bulk surface as well as non-woven microfabric scaffolds. Excellent cell proliferation and viability was observed on poly(N-isopropylacylamide)-CNT-polyaniline non-woven microfabric matrices in compare to poly(N-isopropylacylamide)-CNT-polyaniline bulk and commercially available Matrigel™ even with a range of cell lines up to 168 h. Temperature dependent cells detachment behaviour was observed on the poly(N-isopropylacylamide)-CNT-polyaniline scaffolds by varying incubation at below lower critical solution temperature (LCST) of poly(N-isopropylacylamide). The results suggest that poly(N-isopropylacylamide)-CNT-polyaniline non-woven microfabrics could be used as a smart matrices for applications in tissue engineering.
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| 3. |
- Arheimer, Berit, et al.
(författare)
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The IAHS Science for Solutions decade, with Hydrology Engaging Local People IN a Global world (HELPING)
- 2024
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Ingår i: Hydrological Sciences Journal. - 0262-6667 .- 2150-3435.
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Tidskriftsartikel (refereegranskat)abstract
- The new scientific decade (2023-2032) of the International Association of Hydrological Sciences (IAHS) aims at searching for sustainable solutions to undesired water conditions - may it be too little, too much or too polluted. Many of the current issues originate from global change, while solutions to problems must embrace local understanding and context. The decade will explore the current water crises by searching for actionable knowledge within three themes: global and local interactions, sustainable solutions and innovative cross-cutting methods. We capitalise on previous IAHS Scientific Decades shaping a trilogy; from Hydrological Predictions (PUB) to Change and Interdisciplinarity (Panta Rhei) to Solutions (HELPING). The vision is to solve fundamental water-related environmental and societal problems by engaging with other disciplines and local stakeholders. The decade endorses mutual learning and co-creation to progress towards UN sustainable development goals. Hence, HELPING is a vehicle for putting science in action, driven by scientists working on local hydrology in coordination with local, regional, and global processes.
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| 4. |
- Bishnoi, Sunita, et al.
(författare)
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Electrochemical Sensing of Chlorpyrifos, a Carcinogen Responsible for Breast Cancer, in Milk and Plasma of Lactating Mothers
- 2023
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Ingår i: Electroanalysis. - : Wiley. - 1040-0397 .- 1521-4109. ; 35:2
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Tidskriftsartikel (refereegranskat)abstract
- In view of the increase in breast cancer cases at the global level, electrochemical sensing of the carcinogenic pesticide, chlorpyrifos (CPF) in breast milk is proposed. The determination is based on the nucleophilic substitution reaction of pralidoxime (PAM) with CPF. The proposed method offers a linear concentration range of 0.002 to 0.08 μmol/L. The limit of detection and limit of quantification was found to be 0.05×10−9 and 0.167×10−9M, respectively. The offered “unmodified edge plane pyrolytic graphite sensor” proved to be a better substrate than the earlier reported modified sensors. The limit of detection for the proposed method was found to be nearly fifty times lower than reported at modified electrodes. The interference study proved the adequate selectivity of the offered sensor. The sensor has good stability and reproducibility along with high sensitivity. The offered sensor is very useful for cancer hospitals, pesticide industries, and the study of environmental toxicity-related issues.
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| 5. |
- Eapen, Mathew Suji, et al.
(författare)
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Chronic Obstructive Pulmonary Disease and Lung Cancer : Underlying Pathophysiology and New Therapeutic Modalities
- 2018
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Ingår i: Drugs. - : Springer Science and Business Media LLC. - 0012-6667 .- 1179-1950. ; 78:16, s. 1717-1740
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Tidskriftsartikel (refereegranskat)abstract
- Chronic obstructive pulmonary disease (COPD) and lung cancer are major lung diseases affecting millions worldwide. Both diseases have links to cigarette smoking and exert a considerable societal burden. People suffering from COPD are at higher risk of developing lung cancer than those without, and are more susceptible to poor outcomes after diagnosis and treatment. Lung cancer and COPD are closely associated, possibly sharing common traits such as an underlying genetic predisposition, epithelial and endothelial cell plasticity, dysfunctional inflammatory mechanisms including the deposition of excessive extracellular matrix, angiogenesis, susceptibility to DNA damage and cellular mutagenesis. In fact, COPD could be the driving factor for lung cancer, providing a conducive environment that propagates its evolution. In the early stages of smoking, body defences provide a combative immune/oxidative response and DNA repair mechanisms are likely to subdue these changes to a certain extent; however, in patients with COPD with lung cancer the consequences could be devastating, potentially contributing to slower postoperative recovery after lung resection and increased resistance to radiotherapy and chemotherapy. Vital to the development of new-targeted therapies is an in-depth understanding of various molecular mechanisms that are associated with both pathologies. In this comprehensive review, we provide a detailed overview of possible underlying factors that link COPD and lung cancer, and current therapeutic advances from both human and preclinical animal models that can effectively mitigate this unholy relationship.
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| 6. |
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| 7. |
- Mittal, Nitesh, et al.
(författare)
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Exceptionally robust and conductive superhydrophobic free-standing films of mesoporous carbon nanocapsule/polymer composite for multifunctional applications
- 2015
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Ingår i: Carbon. - : Elsevier. - 0008-6223 .- 1873-3891. ; 93:8, s. 492-501
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Tidskriftsartikel (refereegranskat)abstract
- A novel mesoporous carbon nanocapsule (MCC)/polyvinylidene fluoride (PVDF) polymer composite based free-standing film with multifunctional properties is fabricated by a facile solution approach that is appropriate for dip coating, brush-on and spray applications. The MCC/PVDF composite film exhibits superhydrophobic properties with a water contact angle of approximately 160° and a sliding angle of 5°. The films have a high thermal stability (up to 350 °C in oxidative atmosphere) and are also electrically conductive (tunable from ∼10−3 S m−1 to 10−2 S m−1). The superhydrophobicity is retained even in highly corrosive acidic and basic conditions (pH 1.29–13.54, concentrated HNO3 exposure and ammonium hydroxide solution), as well in a wide humidity range (35–83%). The mesoporous carbon containers (∼100 nm–1 μm size) also provide an interesting platform for encapsulating a variety of functional nanomaterials and activate release, thereby adding to the multi-functionality of the superhydrophobic conductive films of exceptional environmental stability, mechanical strength (∼0.1 GPa) and flexibility.
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| 8. |
- Patra, Hirak Kumar, et al.
(författare)
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Inflammation-sensitive in situ smart scaffolding for regenerative medicine
- 2016
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Ingår i: Nanoscale. - : Royal Society of Chemistry. - 2040-3364 .- 2040-3372. ; 8:39, s. 17213-17222
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Tidskriftsartikel (refereegranskat)abstract
- To cope with the rapid evolution of the tissue engineering field, it is now essential to incorporate the use of on-site responsive scaffolds. Therefore, it is of utmost importance to find new 'Intelligent' biomaterials that can respond to the physicochemical changes in the microenvironment. In this present report, we have developed biocompatible stimuli responsive polyaniline-multiwalled carbon nanotube/poly(N-isopropylacrylamide), (PANI-MWCNT/PNIPAm) composite nanofiber networks and demonstrated the physiological temperature coordinated cell grafting phenomenon on its surface. The composite nanofibers were prepared by a two-step process initiated with an assisted in situ polymerization followed by electrospinning. To obtain a smooth surface in individual nanofibers with the thinnest diameter, the component ratios and electrospinning conditions were optimized. The temperature-gated rearrangements of the molecular structure are characterized by FTIR spectroscopy with simultaneous macromolecular architecture changes reflected on the surface morphology, average diameter and pore size as determined by scanning electron microscopy. The stimuli responsiveness of the nanofibers has first been optimized with computational modeling of temperature sensitive components (coil-like and globular conformations) to tune the mechanism for temperature dependent interaction during in situ scaffolding with the cell membrane. The nanofiber networks show excellent biocompatibility, tested with fibroblasts and also show excellent sensitivity to inflammation to combat loco-regional acidosis that delay the wound healing process by an in vitro model that has been developed for testing the proposed responsiveness of the composite nanofiber networks. Cellular adhesion and detachment are regulated through physiological temperature and show normal proliferation of the grafted cells on the composite nanofibers. Thus, we report for the first time, the development of physiological temperature gated inflammation-sensitive smart biomaterials for advanced tissue regeneration and regenerative medicine.
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| 9. |
- Patra, Santanu, et al.
(författare)
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2-Dimensional graphene as a route for emergence of additional dimension nanomaterials
- 2017
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Ingår i: Biosensors & bioelectronics. - : ELSEVIER ADVANCED TECHNOLOGY. - 0956-5663 .- 1873-4235. ; 89
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Tidskriftsartikel (refereegranskat)abstract
- Dimension has a different and impactful significance in the field of innovation, research and technologies. Starting from one-dimension, now, we all are moving towards 3-D visuals and try to do the things in this dimension. However, we still have some very innovative and widely applicable nanomaterials, which have tremendous potential in the form of 2-D only i.e. graphene. In this review, we have tried to incorporate the reported pathways used so far for modification of 2-D graphene sheets to make is three-dimensional. The modified graphene been applied in many fields like supercapacitors, sensors, catalysis, energy storage devices and many more. In addition, we have also incorporated the conversion of 2-D graphene to their various other dimensions like zero-, one- or three-dimensional nanostructures. (C) 2016 Elsevier B.V. All rights reserved.
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| 10. |
- Pugazhendhi, Arivalagan, et al.
(författare)
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Sugar cane bagasse hydrolysate (SBH) as a lucrative carbon supplement to upgrade the lipid and fatty acid production in Chlorococcum sp. for biodiesel through an optimized binary solvent system
- 2024
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Ingår i: Environmental Research. - : Elsevier. - 0013-9351 .- 1096-0953. ; 241
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Tidskriftsartikel (refereegranskat)abstract
- Cost is the crucial impediment in commercializing microalgal biodiesel. Therefore, cultivating microalgae in cost-effective nutrients reduces the upstream process cost remarkably. Thus, in this study, sugar cane bagasse hydrolysate (SBH) as a lucrative carbon supplement for Chlorococcum sp. and subsequent lipid extraction via an optimized solvent system for biodiesel production was investigated. Characterization of SBH revealed the presence of various monosaccharides and other sugar derivatives such as glucose, fructose, xylose, arabinose, etc. The maximum dry cell weight of 1.7 g/L was estimated in cultures grown in 10 mL SBH. Different solvents such as diethyl ether (DEE), chloroform (CHL), ethyl acetate (ETA), hexane (HEX), methanol (MET), ethanol (ETOH), acetone (ACE) and also combination of solvents (2:1 ratio) such as DEE: MET, CHL: MET, HEX: MET, HEX: ETOH was tested for lipid extraction efficacy. Among solvents used, 12.3% and 18.4% of lipids were extracted using CHL and CHL: MET, respectively, from 10 mL SBH amended cultures. However, the biodiesel yield was found to be similar at about 70.16 % in both SBH and no SBH-added cultures. The fatty acid profile of the biodiesel shows palmitic, oleic, linoleic, linolenic, and arachidonic acid as principal fatty acids. Further, the levels of SFAs, MUFAs, and PUFAs in 10 mL SBH-added cells were 24.67, 12.89, and 34.24%, respectively. Eventually, the fuel properties of Chlorococcum sp. biodiesel, satisfying international biodiesel standards, make the biodiesel a viable diesel substitute in the future.
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| 11. |
- Rai, Radheshyam, et al.
(författare)
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Experimental Techniques: An Introductory Overview
- 2012
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Ingår i: Synthesis, Characterization and Application of Smart Materials. - USA : Nova Science Publishers, Inc.. - 9781614706427 ; , s. 15-30
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Bokkapitel (övrigt vetenskapligt/konstnärligt)abstract
- Smart materials, one of the more focused points in materials research, deal primarily with the chemistry, physics and applications of materials in the real world because it induces conformational changes in complex structures and properties which are useful for the control of them. The thrust area of these types of materials are the combination of functional properties like thermal, electric, magnetic, superconducting and optical, which have led to the development of a wide range of new technological devices. These types of materials have been found to be very useful and interesting for various solid state devices. This book examines research developments of smart materials, including processing, properties and applications, which include device materials and environmentally friendly materials. (Imprint: Nova)
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| 12. |
- Rai, Radheshyam, et al.
(författare)
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Synthesis and Characterization of Bi, Fe, Al and Sb- Modified PLZT
- 2012
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Ingår i: Synthesis, Characterization and Application of Smart Materials. - USA : Nova Science Publishers, Inc.. - 9781614706427 ; , s. 31-98
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Bokkapitel (övrigt vetenskapligt/konstnärligt)abstract
- Smart materials, one of the more focused points in materials research, deal primarily with the chemistry, physics and applications of materials in the real world because it induces conformational changes in complex structures and properties which are useful for the control of them. The thrust area of these types of materials are the combination of functional properties like thermal, electric, magnetic, superconducting and optical, which have led to the development of a wide range of new technological devices. These types of materials have been found to be very useful and interesting for various solid state devices. This book examines research developments of smart materials, including processing, properties and applications, which include device materials and environmentally friendly materials. (Imprint: Nova)
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| 13. |
- Roy, Ekta, et al.
(författare)
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Introduction of selectivity and specificity to graphene using an inimitable combination of molecular imprinting and nanotechnology
- 2017
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Ingår i: Biosensors & bioelectronics. - : ELSEVIER ADVANCED TECHNOLOGY. - 0956-5663 .- 1873-4235. ; 89, s. 234-248
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Tidskriftsartikel (refereegranskat)abstract
- Recently, the nanostructured modified molecularly imprinting polymer has created a great attention in research field due to its excellent properties such as high surface to volume ratio, low cost, and easy preparation/handling. Among the nanostructured materials, the carbonaceous material such as graphene has attracted the tremendous attention of researchers owing to their fascinating electrical, thermal and physical properties. In this review article, we have tried to explore as well as compile the role of graphene-based nanomaterials in the fabrication of imprinted polymers. In other words, herein the recent efforts made to introduce selectivity in graphene-based nanomaterials were tried collected together. The major concern of this review article is focused on the sensing devices fabricated via a combination of graphene, graphene@nanoparticles, graphene@carbon nanotubes and molecularly imprinted polymers. Additionally, the combination of graphene and quantum dots was also included to explore the fluorescence properties of zero-band-gap graphene. (C) 2016 Elsevier B.V. All rights reserved.
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| 14. |
- Roy, Ekta, et al.
(författare)
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RETRACTED: Single cell imprinting on the surface of Ag-ZnO bimetallic nanoparticle modified graphene oxide sheets for targeted detection, removal and photothermal killing of E. Coli
- 2017
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Ingår i: Biosensors & bioelectronics. - : ELSEVIER ADVANCED TECHNOLOGY. - 0956-5663 .- 1873-4235. ; 89, s. 620-626
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Tidskriftsartikel (refereegranskat)abstract
- A very cost-effective, fast, sensitive and specific imprinted polymer modified electrochemical sensor for the targeted detection, removal and destruction of Escherichia coli bacteria was developed onto the surface of Ag-ZnO bimetallic nanoparticle and graphene oxide nanocomposite. The nanocomposite played a dual role in this work, as a platform for imprinting of bacteria as well as a participated in their laser-light induced photo killing. In terms of sensing, our proposed sensor can detect E. Coli as few as 10 CFU mL(-1) and capture 98% of bacterial cells from their very high concentrated solution (10(5) CFU mL(-1)). Similarly to the quantitative detection, we have also investigated the quantitative destruction of E. Coli and found that 16.0 cm(2) area of polymer modified glass plate is sufficient enough to kill 10(5) CFU mL(-1) in the small time span of 5 minutes. The obtained results suggest that our proposed sensor have potential to serve as a promising candidate for specific and quantitative detection, removal as well as the destruction of a variety of bacterial pathogens. (C) 2015 Elsevier B.V. All rights reserved.
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| 15. |
- Sharma, Deepali, et al.
(författare)
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Studies on Bacterial Proteins Corona Interaction with Saponin Imprinted ZnO Nanohoneycombs and Their Toxic Responses
- 2015
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Ingår i: ACS Applied Materials and Interfaces. - : AMER CHEMICAL SOC. - 1944-8244 .- 1944-8252. ; 7:43, s. 23848-23856
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Tidskriftsartikel (refereegranskat)abstract
- Molecular imprinting generates robust, efficient, and highly mesoporous surfaces for biointeractions. Mechanistic interfacial interaction between the surface of core substrate and protein corona is crucial to understand the substantial microbial toxic responses at a nanoscale. In this study, we have focused on the mechanistic interactions between synthesized saponin imprinted zinc oxide nanohoneycombs (SIZnO NHs), average size 80-125 nm, surface area 20.27 m(2)/g, average pore density 0.23 pore/nm and number-average pore size 3.74 nm and proteins corona of bacteria. The produced SIZnO NHs as potential antifungal and antibacterial agents have been studied on Sclerotium rolfsii (S. rolfsii), Pythium debarynum (P. debarynum) and Escherichia coil (E. coli), Staphylococcus aureus (S. aureus), respectively. SIZnO NHs exhibited the highest antibacterial (similar to 50%) and antifungal (similar to 40%) activity against Gram-negative bacteria (E. coil) and fungus (P. debarynum), respectively at concentration of 0.1 mol. Scanning electron spectroscopy (SEM) observation showed that the ZnO NHs ruptured the cell wall of bacteria and internalized into the cell. The molecular docking studies were carried out using binding proteins present in the gram negative bacteria (lipopolysaccharide and lipocalin Blc) and gram positive bacteria (Staphylococcal Protein A, SpA). It was envisaged that the proteins present in the bacterial cell wall were found to interact and adsorb on the surface of SIZnO NHs thereby blocking the active sites of the proteins used for cell wall synthesis. The binding affinity and interaction energies were higher in the case of binding proteins present in gram negative bacteria as compared to that of gram positive bacteria. In addition, a kinetic mathematical model (KMM) was developed in MATLAB to predict the internalization in the bacterial cellular uptake of the ZnO NHs for better understanding of their controlled toxicity. The results obtained from KMM exhibited a good agreement with the experimental data. Exploration of mechanistic interactions, as well as the formation of bioconjugate of proteins and ZnO NHs would play a key role to interpret more complex biological systems in nature.
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| 16. |
- Sharma, Yashpal, et al.
(författare)
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Conducting Polymer Composites for Tissue Engineering Scaffolds
- 2012
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Ingår i: Biomedical Materials and Diagnostic Devices. - USA : John Wiley & Sons. - 9781118030141 ; , s. 597-510
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Bokkapitel (övrigt vetenskapligt/konstnärligt)abstract
- "The functional materials with the most promising outlook have the ability to precisely adjust the biological phenomenon in a controlled mode. Engineering of advanced bio- materials has found striking applications in used for biomedical and diagnostic device applications, such as cell separation, stem-cell, drug delivery, hyperthermia, automated DNA extraction, gene targeting, resonance imaging, biosensors, tissue engineering and organ regeneration"--Provided by publisher.
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| 17. |
- Sharma, Yashpal, et al.
(författare)
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Electrospun Nanofiberfor Three Dimensional Cell Culture
- 2013
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Ingår i: Nanomaterials in Drug Delivery, Imaging, and Tissue Engineering. - USA : John Wiley & Sons. - 9781118290323 ; , s. 417-434
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Bokkapitel (övrigt vetenskapligt/konstnärligt)abstract
- "Nanoscopic therapeutic systems incorporate therapeutic agents, molecular targeting and diagnostic imaging capabilities and are emerging as the next generation of multifarious nanomedicine to improve the therapeutic outcome including chemo and translational therapy. This reference work is one of the first to cover Nanotheragnostics which is the new edge of nanomedicine combining both diagnostic and therapeutic elements at nano level. This large multidisciplinary reference work is has four main parts: Biocompatible Nanomaterials; Nanomedicine: Drug Gene and Cell Delivery; Multi-functional Nanocarrier: Diagnosis, Imaging and Treatment; Tissue Engineering/Regenerative Medicine"--
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