| 1. |
- Beal, Jacob, et al.
(författare)
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Robust estimation of bacterial cell count from optical density
- 2020
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Ingår i: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
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Tidskriftsartikel (refereegranskat)abstract
- Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
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| 2. |
- Chattopadhyay, Tanmoy, et al.
(författare)
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Prompt Emission Polarimetry of Gamma-Ray Bursts with the AstroSat CZT Imager
- 2019
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Ingår i: Astrophysical Journal. - : Institute of Physics (IOP). - 0004-637X .- 1538-4357. ; 884:2
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Tidskriftsartikel (refereegranskat)abstract
- X-ray and gamma-ray polarization measurements of the prompt emission of gamma-ray bursts (GRBs) are believed to be extremely important for testing various models of GRBs. So far, the available measurements of hard X-ray polarization of GRB prompt emission have not significantly constrained the GRB models, particularly because of the difficulty of measuring polarization in these bands. The CZT Imager (CZTI) on board AstroSat is primarily an X-ray spectroscopic instrument that also works as a wide-angle GRB monitor due to the transparency of its support structure above 100 keV. It also has experimentally verified polarization measurement capability in the energy range 100-300 keV and thus provides a unique opportunity to attempt spectropolarimetric studies of GRBs. Here we present the polarization data for the brightest 11 GRBs detected by CZTI during its first year of operation. Among these, five GRBs show polarization signatures with greater than or similar to 3 sigma, and one GRB shows 2 sigma detection significance. We place upper limits for the remaining five GRBs. We provide details of the various tests performed to validate our polarization measurements. While it is difficult yet to discriminate between various emission models with the current sample alone, the large number of polarization measurements that CZTI expects to gather in its minimum lifetime of five years should help to significantly improve our understanding of the prompt emission.
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| 3. |
- Coughlin, Michael W., et al.
(författare)
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GROWTH on S190425z : Searching Thousands of Square Degrees to Identify an Optical or Infrared Counterpart to a Binary Neutron Star Merger with the Zwicky Transient Facility and Palomar Gattini-IR
- 2019
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Ingår i: Astrophysical Journal Letters. - : American Astronomical Society. - 2041-8205 .- 2041-8213. ; 885:1
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Tidskriftsartikel (refereegranskat)abstract
- The third observing run by LVC has brought the discovery of many compact binary coalescences. Following the detection of the first binary neutron star merger in this run (LIGO/Virgo S190425z), we performed a dedicated follow-up campaign with the Zwicky Transient Facility (ZTF) and Palomar Gattini-IR telescopes. The initial skymap of this single-detector gravitational wave (GW) trigger spanned most of the sky observable from Palomar Observatory. Covering 8000 deg(2) of the initial skymap over the next two nights, corresponding to 46% integrated probability, ZTF system achieved a depth of 21 m(AB) in g- and r-bands. Palomar Gattini-IR covered 2200 square degrees in J-band to a depth of 15.5 mag, including 32% integrated probability based on the initial skymap. The revised skymap issued the following day reduced these numbers to 21% for the ZTF and 19% for Palomar Gattini-IR. We narrowed 338,646 ZTF transient ?alerts? over the first two nights of observations to 15 candidate counterparts. Two candidates, ZTF19aarykkb and ZTF19aarzaod, were particularly compelling given that their location, distance, and age were consistent with the GW event, and their early optical light curves were photometrically consistent with that of kilonovae. These two candidates were spectroscopically classified as young core-collapse supernovae. The remaining candidates were ruled out as supernovae. Palomar Gattini-IR did not identify any viable candidates with multiple detections only after merger time. We demonstrate that even with single-detector GW events localized to thousands of square degrees, systematic kilonova discovery is feasible.
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| 4. |
- Sharma, V., et al.
(författare)
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Improved mechanical properties of a Ti-48Al alloy processed by mechanical alloying and spark plasma sintering
- 2023
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Ingår i: Materials Today Communications. - : Elsevier BV. - 2352-4928. ; 35
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Tidskriftsartikel (refereegranskat)abstract
- Bulk Ti-48Al alloy samples were prepared by the high energy ball milling (HBM) of elemental powders, followed by spark plasma sintering (SPS) of the HBM processed powders. The microstructure, phase evolution and mechanical properties of the bulk alloy were studied. The resulting TiAl + Ti3Al two phase alloy possessed an equiaxed fine grain structure, unlike the usual lamellar structure produced by arc melting. The process parameters of HBM and SPS, e.g., milling speed, milling time and sintering temperature were used to tune the phase fraction, microstructure, and grain size. A very high nanohardness of up to ∼12 GPa was obtained, ∼2.4 times higher than the corresponding value of the as-cast counterpart. The combined influence of powder size reduction during HBM, high Ti3Al phase fraction and microstructural development during SPS resulted in higher hardness, wear resistance and yield pressure. Thus, a HBM+SPS processing approach is a promising processing route for the manufacture of high hardness bulk TiAl alloys.
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| 5. |
- Chattopadhyay, Tanmoy, et al.
(författare)
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Sub-MeV spectroscopy with AstroSat-CZT imager for gamma ray bursts
- 2021
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Ingår i: Journal of astrophysics and astronomy. - : Springer Nature. - 0250-6335 .- 0973-7758. ; 42:2
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Tidskriftsartikel (refereegranskat)abstract
- Cadmium-Zinc-Telluride Imager (CZTI) onboard AstroSat has been a prolific Gamma-Ray Burst (GRB) monitor. While the 2-pixel Compton scattered events (100-300 keV) are used to extract sensitive spectroscopic information, the inclusion of the low-gain pixels (similar to 20% of the detector plane) after careful calibration extends the energy range of Compton energy spectra to 600 keV. The new feature also allows single-pixel spectroscopy of the GRBs to the sub-MeV range which is otherwise limited to 150 keV. We also introduced a new noise rejection algorithm in the analysis ('Compton noise'). These new additions not only enhances the spectroscopic sensitivity of CZTI, but the sub-MeV spectroscopy will also allow proper characterization of the GRBs not detected by Fermi. This article describes the methodology of single, Compton event and veto spectroscopy in 100-900 keV combined for the GRBs detected in the first year of operation. CZTI in last five years has detected similar to 20 bright GRBs. The new methodologies, when applied on the spectral analysis for this large sample of GRBs, has the potential to improve the results significantly and help in better understanding the prompt emission mechanism.
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| 6. |
- Chaudhary, Varun, 1984, et al.
(författare)
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Magnetic and mechanical properties of additively manufactured Alx(CoFeNi) complex concentrated alloys
- 2023
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Ingår i: Scripta Materialia. - : Elsevier BV. - 1359-6462. ; 224
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Tidskriftsartikel (refereegranskat)abstract
- Varying the Al content, strongly influences the microstructure, magnetic and microhardness of additively manufactured Alx(CoFeNi) (x = 0, 10, 30) complex concentrated alloys (CCA). Compared to the single FCC phase of CoFeNi, the hierarchical FCC/L12+BCC/B2 heterostructure of heat treated Al10(CoFeNi) CCA displayed substantially improved saturation magnetization, Curie temperature and microhardness. However, there was no significant change in the properties of heat treated CoFeNi and Al30(CoFeNi) CCA. These findings can be rationalized via thermodynamic modelling of the phase stability. We have demonstrated the feasibility of exploiting additive manufacturing for rapidly screening and developing novel high-performance alloys for next generation rotating electrical machines.
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| 7. |
- Kasliwal, Mansi M., et al.
(författare)
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Kilonova Luminosity Function Constraints Based on Zwicky Transient Facility Searches for 13 Neutron Star Merger Triggers during O3
- 2020
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Ingår i: Astrophysical Journal. - : American Astronomical Society. - 0004-637X .- 1538-4357. ; 905:2
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Tidskriftsartikel (refereegranskat)abstract
- We present a systematic search for optical counterparts to 13 gravitational wave (GW) triggers involving at least one neutron star during LIGO/Virgo's third observing run (O3). We searched binary neutron star (BNS) and neutron star black hole (NSBH) merger localizations with the Zwicky Transient Facility (ZTF) and undertook follow-up with the Global Relay of Observatories Watching Transients Happen (GROWTH) collaboration. The GW triggers had a median localization area of 4480 deg(2), a median distance of 267 Mpc, and false-alarm rates ranging from 1.5 to 10(-25) yr(-1). The ZTF coverage in the g and r bands had a median enclosed probability of 39%, median depth of 20.8 mag, and median time lag between merger and the start of observations of 1.5 hr. The O3 follow-up by the GROWTH team comprised 340 UltraViolet/Optical/InfraRed (UVOIR) photometric points, 64 OIR spectra, and three radio images using 17 different telescopes. We find no promising kilonovae (radioactivity-powered counterparts), and we show how to convert the upper limits to constrain the underlying kilonova luminosity function. Initially, we assume that all GW triggers are bona fide astrophysical events regardless of false-alarm rate and that kilonovae accompanying BNS and NSBH mergers are drawn from a common population; later, we relax these assumptions. Assuming that all kilonovae are at least as luminous as the discovery magnitude of GW170817 (-16.1 mag), we calculate that our joint probability of detecting zero kilonovae is only 4.2%. If we assume that all kilonovae are brighter than -16.6 mag (the extrapolated peak magnitude of GW170817) and fade at a rate of 1 mag day(-1) (similar to GW170817), the joint probability of zero detections is 7%. If we separate the NSBH and BNS populations based on the online classifications, the joint probability of zero detections, assuming all kilonovae are brighter than -16.6 mag, is 9.7% for NSBH and 7.9% for BNS mergers. Moreover, no more than <57% (<89%) of putative kilonovae could be brighter than -16.6 mag assuming flat evolution (fading by 1 mag day(-1)) at the 90% confidence level. If we further take into account the online terrestrial probability for each GW trigger, we find that no more than <68% of putative kilonovae could be brighter than -16.6 mag. Comparing to model grids, we find that some kilonovae must have M-ej M, X-lan > 10(-4), or > 30 degrees to be consistent with our limits. We look forward to searches in the fourth GW observing run; even 17 neutron star mergers with only 50% coverage to a depth of -16 mag would constrain the maximum fraction of bright kilonovae to <25%.
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| 8. |
- Lanekoff, Ingela, Prof. 1975-, et al.
(författare)
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Single-cell metabolomics : where are we and where are we going?
- 2022
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Ingår i: Current Opinion in Biotechnology. - : Elsevier. - 0958-1669 .- 1879-0429. ; 75
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Forskningsöversikt (refereegranskat)abstract
- Single-cell metabolomics with mass spectrometry enables a large variety of metabolites to be simultaneously detected from individual cells, without any preselection or labelling, to map phenotypes on the single cell level. Although the field is relatively young, it is steadily progressing with an increasing number of active research groups, techniques for cell sampling and ionization, tools for data analysis, and applications to answer important biomedical and environmental questions. In addition, the community shows great creativity in overcoming challenges associated with low sample volumes, a wide range of metabolite species, and large datasets. Here, we briefly discuss publications since 2019 and aim to provide the unfamiliar reader with an insight into the field and the expert reader with an update on the current status of the field.
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| 9. |
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| 10. |
- Sharma, Vidushi, et al.
(författare)
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Time-varying Polarized Gamma-Rays from GRB 160821A : Evidence for Ordered Magnetic Fields
- 2019
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Ingår i: Astrophysical Journal Letters. - : IOP PUBLISHING LTD. - 2041-8205 .- 2041-8213. ; 882:1
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Tidskriftsartikel (refereegranskat)abstract
- GRB 160821A is the third most energetic gamma-ray burst observed by the Fermi gamma-ray space telescope. Based on the observations made by the Cadmium Zinc Telluride Imager on board AstroSat, here we report the most conclusive evidence to date of (i) high linear polarization (66(27)(+26)%; 5.3 sigma detection), and (ii) variation of polarization angle with time, occurring twice during the rise and decay phase of the burst at 3.5 sigma and 3.1 sigma detections, respectively. All confidence levels are reported for two parameters of interest. These observations strongly suggest synchrotron radiation produced in magnetic field lines that are highly ordered on angular scales of 1/Gamma, where Gamma is the Lorentz factor of the outflow.
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| 11. |
- Varahabhatla, S. M., et al.
(författare)
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Influence of annealing on enhancing soft magnetic properties in laser powder bed fusion processed Hiperco (Fe-49Co-2V)
- 2024
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Ingår i: Additive Manufacturing Letters. - 2772-3690. ; 9
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Tidskriftsartikel (refereegranskat)abstract
- Annealing of laser powder bed fusion (LPBF) processed Fe-49Co-2 V (Hiperco) samples at 865 °C for 4 h leads to a substantial improvement in its soft magnetic properties. While the as built LPBF samples exhibited relatively higher coercivities (Hc) ranging from 25.8 – 26.5 Oe, the annealed LPBF samples showed significantly lower coercivities (Hc) of 6.9 – 10.8 Oe. These lower Hc values can not only be attributed to the 15–20 times larger grain sizes, but also the higher degree of B2 ordering in the annealed condition. The enhanced degree of B2 ordering also increases the saturation magnetization (Ms), from 213 to 228 emu/g, in samples processed with a laser fluence (energy/density) of 3.4 J/mm2. These results reveal the underlying mechanisms leading to an enhancement of soft magnetic properties in LPBF processed Hiperco via annealing-induced microstructural control.
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