| 1. |
- Sharov, Andrei A, et al.
(författare)
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Noggin overexpression inhibits eyelid opening by altering epidermal apoptosis and differentiation.
- 2003
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Ingår i: The EMBO journal. - : Wiley. - 0261-4189 .- 1460-2075. ; 22:12, s. 2992-3003
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Tidskriftsartikel (refereegranskat)abstract
- Contact of developing sensory organs with the external environment is established via the formation of openings in the skin. During eye development, eyelids first grow, fuse and finally reopen, thus providing access for visual information to the retina. Here, we show that eyelid opening is strongly inhibited in transgenic mice overexpressing the bone morphogenetic protein (BMP) antagonist noggin from the keratin 5 (K5) promoter in the epidermis. In wild-type mice, enhanced expression of the kinase-inactive form of BMPR-IB mediated by an adenovirus vector also inhibits eyelid opening. Noggin overexpression leads to reduction of apoptosis and retardation of cell differentiation in the eyelid epithelium, which is associated with downregulation of expression of the apoptotic receptors (Fas, p55 kDa TNFR), Id3 protein and keratinocyte differentiation markers (loricrin, involucrin). BMP-4, but not EGF or TGF-alpha, accelerates opening of the eyelid explants isolated from K5-Noggin transgenic mice when cultured ex vivo. These data suggest that the BMP signaling pathway plays an important role in regulation of genetic programs of eyelid opening and skin remodeling during the final steps of eye morphogenesis.
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| 2. |
- Botchkarev, Vladimir A, et al.
(författare)
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Modulation of BMP signaling by noggin is required for induction of the secondary (nontylotrich) hair follicles.
- 2002
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Ingår i: The Journal of investigative dermatology. - : Elsevier BV. - 0022-202X. ; 118:1, s. 3-10
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Tidskriftsartikel (refereegranskat)abstract
- Increasing evidence suggests that morphogenesis of the distinct developmental structures derived from the same organ-committed epithelium is controlled by differential mechanisms. As was recently shown in mice with mutations in the downless (dL) gene, induction of primary or tylotrich hair follicles is strikingly dependent of signaling through the Tnf receptor homologue, Edar. Here, we show that dorsal skin of murine embryos with constitutive deletion of the BMP2/4 antagonist noggin, after transplantation into SCID mice, is characterized by the lack of induction of secondary hair follicles, and by the arrest of primary hair follicle development prior to hair shaft formation. The loss of noggin activity was associated with failure to express genes that specify hair follicle cell fates in the epidermis (Lef-1, beta-catenin, Shh) and dermal papilla (p75 kDa neurotrophin receptor, alkaline phosphatase). This suggests that regulation of BMP2/4 signaling by noggin is essential for the induction of secondary hair follicles, as well as for advanced stages of development in primary hair follicles.
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| 3. |
- Samoilov, A. M., et al.
(författare)
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Vaporization and thermodynamic properties of indium-lead melts
- 2008
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Ingår i: Russian journal of general chemistry. - 1070-3632 .- 1608-3350. ; 78:1, s. 1-5
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Tidskriftsartikel (refereegranskat)abstract
- Vapor composition and thermodynamic properties of melts of the In-Pb system were studied by high-temperature mass spectrometry. In the temperature range 780-1170 K, the In1-xPbx melts feature an appreciable positive deviation from the ideal behavior.
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