| 1. |
- Carrasco, Anna, et al.
(författare)
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The Tonsil Lymphocyte Landscape in Pediatric Tonsil Hyperplasia and Obstructive Sleep Apnea
- 2021
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Ingår i: Frontiers in Immunology. - : Frontiers Media S.A.. - 1664-3224. ; 12
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Tidskriftsartikel (refereegranskat)abstract
- Tonsil hyperplasia is the most common cause of pediatric obstructive sleep apnea (OSA). Despite the growing knowledge in tissue immunology of tonsils, the immunopathology driving tonsil hyperplasia and OSA remains unknown. Here we used multi-parametric flow cytometry to analyze the composition and phenotype of tonsillar innate lymphoid cells (ILCs), T cells, and B cells from pediatric patients with OSA, who had previous polysomnography. Unbiased clustering analysis was used to delineate and compare lymphocyte heterogeneity between two patient groups: children with small tonsils and moderate OSA (n = 6) or large tonsils and very severe OSA (n = 13). We detected disturbed ILC and B cell proportions in patients with large tonsils, characterized by an increase in the frequency of naive CD27(-)CD21(hi) B cells and a relative reduction of ILCs. The enrichment of naive B cells was not commensurate with elevated Ki67 expression, suggesting defective differentiation and/or migration rather than cellular proliferation to be the causative mechanism. Finally, yet importantly, we provide the flow cytometry data to be used as a resource for additional translational studies aimed at investigating the immunological mechanisms of pediatric tonsil hyperplasia and OSA.
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| 2. |
- Friberg, Danielle, et al.
(författare)
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Nytt vårdförlopp för barn med OSDB är godkänt att tas i bruk. Obstruktiv sömnrelaterad andningsstörning – ett vanligt och potentiellt allvarligt tillstånd : [New Swedish National care process for pediatric obstructive sleep disordered breathing]
- 2023
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Ingår i: Läkartidningen. - : Sveriges läkarförbund. - 0023-7205 .- 1652-7518. ; 120
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Tidskriftsartikel (refereegranskat)abstract
- Obstructive sleep disordered breathing (OSDB) is a spectrum from habitual snoring and labored breathing to obstructive sleep apnea (OSA), which is common and potentially serious in children. The process contains a new question at child care centers, directed at caretakers with children at age 18 months and 3 years, concerning habitual snoring (3 times a week or more). A primary care doctor verifies the suspicion of OSDB in case of a positive answer to one of 7 additional questions or 4 status findings (e.g. tonsil hypertrophy). The process starts with the suspicion of OSDB, from the age of 18 months to 18 years, and ends when symptoms are improved after watchful waiting or upper airway surgery. National equality is a goal, with increased access to nocturnal respiratory recordings of children with comorbidities or doubtful cases. Also, with short waiting time to first visit at ORL department, and to surgery. Children with comorbidities or severe symptoms get postoperative follow-ups with a nurse after 6 months. The new ICD code for OSDB is R06.8A.
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| 3. |
- Kisiel, Marta, 1984-, et al.
(författare)
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Development of bacterial resistance during treatment with topical gentamicin for chronic rhinosinusitis in patients with cystic fibrosis and primary ciliary dyskinesis. Retrospective case series
- 2020
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Ingår i: POLISH JOURNAL OF OTOLARYNGOLOGY. - : INDEX COPERNICUS SP ZOO. - 0030-6657 .- 2300-8423. ; 74:3, s. 33-40
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Tidskriftsartikel (refereegranskat)abstract
- Background: The management of chronic rhinosinusitis (CRS) in patients with cystic fibrosis (CF) and primary ciliary dyskinesia (PCD) is still a challenge. At our institution we have used gentamycin nasal spray, extemporaneously produced, for prophylactic treatment of moderate-to-severe CRS. The aim of this study was to investigate the gentamycin susceptibility of bacteria in sputum samples in CF and PCD patients treated for CRS.Methodology: Patients with CF and PCD who were prescribed gentamycin nasal spray for CRS and had sputum bacterial cultures taken pre-treatment and followed-up at least once after >= 6 months were retrospectively included. Microbiological data were descriptively analysed in terms of bacterial species and resistance to gentamycin.Results: A case series of 17 CF and 12 PCD patients passed the inclusion criteria. Of those cases, three (18%) CF patients and one (8%) PCD patient developed resistance to gentamycin during treatment with gentamycin nasal spray. In all four cases, the resistant bacterial isolates were P. aeruginosa. Additionally, two CF patients already had P. aeruginosa isolates resistant to gentamycin in the pre-treatment culture. In further two CF patients, the multi-resistant Burgdorferi cepacia complex, including gentamycin resistance, was identified. P. aeruginosa and S. aureus in CF and P. aeruginosa and H. influenza in PCD were the predominant bacterial species.Conclusions: The study showed that there was moderate incidence of gentamycin resistance in CF and PCD patients at our institution. However, further prospective studies are needed to confirm the outcomes.
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| 4. |
- Sjölander, Isabella, et al.
(författare)
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Adenotonsillotomy versus adenotonsillectomy in pediatric obstructive sleep apnea : A 5-year RCT
- 2022
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Ingår i: Sleep medicine: X. - : Elsevier. - 2590-1427. ; 4
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Tidskriftsartikel (refereegranskat)abstract
- Objectives: Adenotonsillectomy (ATE) is a common treatment for pediatric obstructive sleep apnea (OSA). Intracapsular adenotonsillotomy (ATT) is associated with less postoperative morbidity. Our previous randomized controlled trial (RCT) compared ATE and ATT in otherwise healthy children with moderate to severe OSA. No differences in polysomnographic (PSG) and OSA-18 were found between the groups at one-year follow-up. This study presents the long-term results of the RCT.Methods: Non-obese children (n = 79, 2-6 years) who had undergone either ATE (n = 40) or ATT (n = 39) were offered PSG and OSA-18 questionnaire five-years after surgery. Primary outcome was the group difference in postoperative Obstructive Apnea/Hypopnea Index (OAHI). ATE was recommended to the ATT group if they had a relapse of OSA.Results: The follow-up was completed by 45 of 79 (57%) children; 28 (35%) drop-outs, and six of 39(15%) in the ATT group were excluded after ATE. After ATE(n = 17), OAHI decreased from mean 12.3(SD 8.0) to 0.6(0.7), and after ATT(n = 28) from 12.6(7.4) to 0.5(0.6), a mean difference in postoperative OAHI of 0.1(95% CI -0.3 - 0.5). Sensitivity analyses did not change the results. The median OSA-18 decreased in the ATE group from 57(interquartile range 47-79) to 27(22-36), and in the ATT group from 67(53-79) to 32(25-44), without group differences for postoperative values.Conclusion: The results of this five-year follow-up of otherwise healthy OSA-children showed a high drop-out rate, but indicates that ATT could be an effective treatment for pediatric OSA. However, ATT warrants follow-up due to the risk of recurrence, and further studies are needed.
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| 5. |
- Sjölander, Isabella, et al.
(författare)
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Correlations between objective and subjective outcomes after adenotonsillar surgery in children with OSA
- 2022
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Ingår i: Laryngoscope Investigative Otolaryngology (LIO). - : John Wiley & Sons. - 2378-8038. ; 7:6, s. 2161-2170
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Tidskriftsartikel (refereegranskat)abstract
- ObjectivesTo investigate whether the OSA-18 questionnaire and a postoperative patient-reported outcome measure (PROM) question correlated with polysomnography (PSG) data.MethodsA prospective study of otherwise healthy young children with moderate to severe obstructive sleep apnea (OSA) to investigate if the obstructive apnea–hypopnea index (OAHI) before and 6–12 months after adenotonsil surgery correlated with the OSA-18 total symptom score (TSS) and the sleep disturbance subscale (SDS), as well as a PROM question on symptom improvement with responses on a 4-grade Likert scale.ResultsOf 201 children, 173 (86%) had complete data of OAHI and OSA-18 pre- and postoperatively. The mean age was 3.2 years (SD 1.0) and the mean OAHI was 15.9 (11.3). Significant correlations between changes in the OAHI and OSA-18 were found, both TSS (r = 0.29, p < .001) and SDS (r = 0.53, p < .001). A total of 136 (68%) patients responded to the PROM question, the majority of whose symptoms had disappeared (n = 102) or almost disappeared (n = 30). Four patients had unchanged symptoms, and none had worsening symptoms. A correlation was found between the PROM question and a change in the OAHI (r = 0.36, p < .001), as well as a change in the OSA-18 TSS (r = 0.24, p = .006) and the SDS (r = 0.34, p < .001). The specificity of the PROM question for prediction of a postoperative OAHI < 2 was 82%, and the sensitivity was 38%.ConclusionChanges in the OAHI significantly correlated with changes in the OSA-18, especially with the sleep disturbance scale, which could be an alternative for evaluation at follow-ups.
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| 6. |
- Sjölander, Isabella
(författare)
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Pediatric Obstructive Sleep Apnea : Evaluation of surgical treatments and immunological aspects
- 2022
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Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- Pediatric obstructive sleep apnea (OSA) is a sleep-related breathing disorder often caused by enlargement of the adenoid and the tonsils. The peak prevalence is among children who are two to six years of age, when the lymphatic tissue is most active. Clinical diagnosis can be challenging, since the symptoms are often unspecific. Polysomnography, the diagnostic gold standard is a complex procedure with limited availability.The standard surgical treatment for OSA is removal of the tonsils (tonsillectomy) together with removal of the adenoid (adenoidectomy). Partial removal of the tonsils together with adenoidectomy, known as adenotonsillotomy, is a treatment accompanied with less postoperative morbidity but a higher risk of reoperation. The aims of this thesis are to evaluate different treatment methods for treating pediatric OSA and to investigate immunological aspects of tonsil hypertrophy.In Paper I, tonsil tissue from children with large tonsils and moderate to severe OSA was compared with tonsil tissue from children with small tonsils and milder OSA. The tissue was analyzed with flow-cytometry using markers for T cells, B cells and ILCs. Patients with enlarged tonsils and more severe OSA had a significant increase of naïve B cells without a difference in the Ki67 proliferation marker. This indicates an impaired differentiation and/or migration of B cells in the larger tonsils.In Paper II, behavior and mental health after adenotonsillectomy or adenotonsillotomy were compared. Together with polysomnography and the questionnaire OSA-18, a strength and difficulties questionnaire (SDQ) was filled out both before and one year after treatment. No significant differences between the treatment groups were seen after one year, in terms of mental health and behavior. Paper III is a long-term follow-up study of a previously published randomized controlled trial of adenotonsillectomy and adenotonsillotomy. Five years after surgery, a new polysomnography was performed on the patients. The results showed a high dropout rate, and six children were excluded because of re-operation with adenotonsillectomy. No difference in the obstructive apnea hypopnea index was found between the groups five years after surgical intervention.Paper IV is a prospective cohort study of children treated for OSA. The correlations between subjective data from OSA-18, patient-reported outcome measures, and objective data from polysomnography were compared. Significant correlations were shown between changes in objective polysomnography data and changes in OSA-18, with the strongest correlation in the sleep disturbance subscale. The measure of patient-reported outcomes showed a significant but weak correlation.
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| 7. |
- Sjölander, Isabella, et al.
(författare)
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Randomised trial showed no difference in behavioural symptoms between surgical methods treating paediatric obstructive sleep apnoea
- 2020
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Ingår i: Acta Paediatrica. - : Wiley. - 0803-5253 .- 1651-2227. ; 109:10, s. 2099-2104
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Tidskriftsartikel (refereegranskat)abstract
- AIM: Our previous randomised controlled trial of children with obstructive sleep apnoea (OSA) showed no significant differences between adenotonsillectomy (ATE) and adenotonsillotomy (ATT) in improving nocturnal respiration and quality of life after 1 year. The aim of this report was to evaluate the effects on behavioural symptoms using the Strengths and Difficulties Questionnaire (SDQ).METHODS: Children between 2 and 6 years with OSA were randomised to ATT or ATE. Parents, blinded to method, answered the SDQ while their child underwent polysomnography before and 1 year after surgery. Differences between the total SDQ scores were analysed between the treatment groups.RESULTS: The SDQ was filled out in 87% of the cases preoperatively, and in 86% postoperatively. At follow-up, the mean total SDQ score was 9.6 SD ± 5.1 in the ATE group (n = 31), and 8.2 ± 6.7 in the ATT group (n = 37), P = .09. The mean total SDQ score for all was preoperatively 10.6 ± 5.0, and postoperatively 8.8 ± 6.0, P = .0002.CONCLUSION: There were no significant differences in SDQ scores between the groups at follow-up, indicating that the more conservative ATT is a treatment option in paediatric OSA. The whole group of patients showed a significant improvement after surgery.
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