| 1. |
- Moberg, Christina, et al.
(författare)
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De unga gör helt rätt när de stämmer staten
- 2022
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Ingår i: Aftonbladet. - 1103-9000.
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Tidskriftsartikel (populärvet., debatt m.m.)abstract
- 1 620 forskare och lärare i forskarvärlden: Vi ställer oss bakom Auroras klimatkrav
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| 2. |
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| 3. |
- Alveteg, Mattias, et al.
(författare)
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Vad räknas som belägg för studenters måluppfyllelse?
- 2018
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Ingår i: 10:e Pedagogiska Inspirationskonferensen 2018. - 2003-3761 .- 2003-377X. ; 10
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Konferensbidrag (refereegranskat)abstract
- Universitetskanslerämbetet (UKÄ) ställer numera krav på svenska lärosäten att vi ska utvärdera oss själva. Vi på LTH bör då gemensamt försöka hitta sätt att uppfylla UKÄs krav som även ger de verktyg vi själva behöver för att förbättra våra utbildningars kvalitét.Hur tar vi reda på våra utbildningars styrkor och svagheter och vad vi kan göra för att förbättra dem ytterligare? Ett sätt är att undersöka hur väl studenterna uppfyller examensmålen. Vad som ska räknas som belägg för studenters måluppfyllelse är dock svårt, av flera olika skäl. Vår rekommendation blir att triangulera olika typer av belägg samt att tydligt involvera institutionerna i arbetet.
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| 4. |
- Andersson, C., et al.
(författare)
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Improved systems for hydrophobic tagging of recombinant immunogens for efficient iscom incorporation
- 2000
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Ingår i: JIM - Journal of Immunological Methods. - 0022-1759 .- 1872-7905. ; 238:02-jan, s. 181-193
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Tidskriftsartikel (refereegranskat)abstract
- We have previously reported a strategy for production in Escherichia coli of recombinant immunogens fused to a hydrophobic tag to improve their capacity to associate with an adjuvant formulation [Andersson et al., J. Immunol. Methods 222 (1999) 171]. Here, we describe a further development of the previous strategy and present significant improvements. In the novel system, the target immunogen is produced with an N-terminal affinity tag suitable for affinity purification, and a C-terminal hydrophobic tag, which should enable association through hydrophobic interactions of the immunogen with an adjuvant system, here being immunostimulating complexes (iscoms). Two different hydrophobic tags were evaluated: (i) a tag denoted M, derived from the membrane-spanning region of Staphylococcus aureus protein A (SpA), and (ii) a tag denoted MI consisting of the transmembrane region of hemagglutinin from influenza A virus. Furthermore, two alternative affinity tags were evaluated; the serum albumin-binding protein ABP, derived from streptococcal protein G, and the divalent IgG-binding ZZ-domains derived from SpA. A malaria peptide M5, derived from the central repeat region of the Plasmodium falciparum blood-stage antigen Pf155/RESA, served as model immunogen in this study. Four different fusion proteins, ABP-MS-M, ABP-MS-MI, ZZ-MS-M and ZZ-MS-MI, were thus produced, affinity purified and evaluated in iscom-incorporation experiments. All of the fusion proteins were found in the iscom fractions in analytical ultracentrifugation, indicating iscom incorporation. This was further supported by electron microscopy analysis showing that iscoms were formed. In addition, these iscom preparations were demonstrated to induce MS-specific antibody responses upon immunisation of mice, confirming the successful incorporation into iscoms. The novel system for hydrophobic tagging of immunogens, with optional affinity and hydrophobic tags, gave expression levels that were increased ten to fifty-fold, as compared to the earlier reported system. We believe that the presented strategy would be a convenient way to achieve efficient adjuvant association for recombinant immunogens.
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| 5. |
- Atamanchuk, Dariia, 1987, et al.
(författare)
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Detection of CO2 leakage from a simulated sub-seabed storage site using three different types of pCO2 sensors
- 2015
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Ingår i: International Journal of Greenhouse Gas Control. - : Elsevier BV. - 1750-5836. ; 38, s. 121-134
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Tidskriftsartikel (refereegranskat)abstract
- This work is focused on results from a recent controlled sub-seabed in situ carbon dioxide (CO2) releaseexperiment carried out during May–October 2012 in Ardmucknish Bay on the Scottish west coast. Threetypes of pCO2sensors (fluorescence, NDIR and ISFET-based technologies) were used in combination withmultiparameter instruments measuring oxygen, temperature, salinity and currents in the water columnat the epicentre of release and further away. It was shown that distribution of seafloor CO2 emissionsfeatures high spatial and temporal heterogeneity. The highest pCO2values (∼1250 atm) were detectedat low tide around a bubble stream and within centimetres distance from the seafloor. Further up in thewater column, 30–100 cm above the seabed, the gradients decreased, but continued to indicate elevatedpCO2at the epicentre of release throughout the injection campaign with the peak values between 400and 740atm. High-frequency parallel measurements from two instruments placed within 1 m fromeach other, relocation of one of the instruments at the release site and 2D horizontal mapping of therelease and control sites confirmed a localized impact from CO2emissions. Observed effects on the watercolumn were temporary and post-injection recovery took <7 days.A multivariate statistical approach was used to recognize the periods when the system was dominatedby natural forcing with strong correlation between variation in pCO2and O2, and when it was influencedby purposefully released CO2.Use of a hydrodynamic circulation model, calibrated with in situ data, was crucial to establishingbackground conditions in this complex and dynamic shallow water system.
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| 6. |
- Bjerke, Axel, et al.
(författare)
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Onset of the degradation of CVD alpha-Al2O3 coating during turning of Ca-treated steels
- 2021
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Ingår i: Wear. - : Elsevier BV. - 0043-1648 .- 1873-2577. ; 477
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Tidskriftsartikel (refereegranskat)abstract
- The ability to control the shape, distribution and composition of non-metallic inclusions has had an important impact on many aspects of steel making. One such impact is on the machinability. Ca-treatments have shown to be able to reduce the abrasiveness of oxide inclusions, improve chip-breaking and lead to formation of deposits that reduce tool wear. However, machining Ca-treated steels with Al2O3 coated cemented carbide tools has not been as advantageous as expected. This study investigates the mechanisms behind the anomalous wear of Al2O3 coatings when turning soft Ca-treated steels. Longitudinal turning tests at a range of speeds (vc = 100-600 m/min) show rapid localized degradation of the Al2O3 coating limited to the sliding zone. Detailed analysis of the degradation mechanisms was performed using scanning and transmission electron microscopy. The results demonstrate a presence of chemical interactions between the alumina coating and non-metallic inclusions. The interaction resulted in the formation of mainly calcium aluminates and partly alumina-magnesia spinel. In-operando infrared thermography measurements indicate cutting temperatures of 850-1000 degrees C. Thermodynamic calculations give that CaO and MgO readily reacts with Al2O3, while the reaction with CaS requires presence of additional oxygen at these cutting conditions. Additional turning experiments investigate the influence of oxygen by controlling the cutting environment by adding oxygen (compressed air) or removing oxygen (supply argon). These additional tests show that the presence of additional oxygen has a limited impact on the possible Ca-Al2O3 interaction. This demonstrat a potential for further machinability improvements by controlling the chemical interaction between Ca and Mg based non-metallic inclusions and alumina coatings.
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| 7. |
- Buechler, Daniela, et al.
(författare)
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A Critical Mapping of Practice-Based Research as Evidenced by Swedish Architectural Theses
- 2011
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Ingår i: International Journal of Art & Design Education. - : Wiley. - 1476-8062. ; 30:2, s. 318-327
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Tidskriftsartikel (refereegranskat)abstract
- This article presents an investigation that was funded by the Swedish Institute into the role of creative practice in architectural research as evidenced in Swedish doctoral theses. The sample was mapped and analysed in terms of clusters of interest, approaches, cultures of knowledge and uses of creative practice. This allowed the identification of the ontological, epistemological and methodological attitudes of the researchers, and hence a glimpse of their implicit worldview. The authors claim that the relationship between worldview and research actions in emerging areas of research such as architecture is often under-scrutinised, resulting in a disjunction between aims and strategies for action. Architectural research in which creative practices make an essential contribution to the aims, might represent something new in academic research. The investigators therefore focused on identifying cases in which creative practice was or was claimed to be integrated in an essential way into the research process. There were two principal conclusions. The first was that the mere presence of practice was not necessarily an indicator of so-called arts-based research. The second was that cases of arts-based research that could be regarded as a new paradigm as opposed to a variant of existing paradigms are less common than is claimed by the researchers themselves. As a result, this article makes a contribution to the debate on whether so-called practice-based research should be regarded as a distinct new paradigm.
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| 8. |
- Diaz-Parejo, P, et al.
(författare)
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Cerebral energy metabolism during transient hyperglycemia in patients with severe brain trauma
- 2003
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Ingår i: Intensive Care Medicine. - : Springer Science and Business Media LLC. - 0342-4642 .- 1432-1238. ; 29:4, s. 544-550
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Tidskriftsartikel (refereegranskat)abstract
- Objective: To study whether transient hyperglycemia adversely affects cerebral energy metabolism in patients with severe traumatic brain lesions. Design and setting: Prospective, nonrandomized study in the neurosurgical intensive care unit of a university hospital. Patients: 108 patients treated for severe traumatic brain lesions. Interventions: All patients were treated according to neurosurgical intensive care routine including monitoring of, intracranial pressure. One microdialysis catheter was inserted via a burr hole frontally to that used for the intraventricular catheter ("better" position). In patients with focal lesions one or more catheters were inserted into cerebral cortex surrounding an evacuated focal contusion or underlying an evacuated hematoma ("worse" position). Perfusion rate was 0.3 mul/min and samples were taken every. 30 or 60 min. The levels of glucose, pyruvate, lactate, glutamate, and glycerol were analyzed and displayed bedside. Measurements and results: There were 18 episodes of moderate (12-15 mmol/1) and 6 episodes of pronounced (>15 mmol/1) hyperglycemia. Moderate hyperglycemia did not change intracerebral levels of lactate, pyruvate, glutamate, glycerol, or lactate/pyruvate ratio. Lactate concentrations increased during pronounced hyperglycemia. Pronounced cerebral lactic acidosis and a moderate increase in interstitial glycerol concentration indicating cell membrane degradation was observed in a single patient with pronounced, long-lasting hyperglycemia. Conclusions: Cerebral energy metabolism was affected by transient hyperglycemia only at blood glucose concentration above 15 mmol/l as shown by a moderate increase in interstitial lactate level.
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| 9. |
- Ehlers, Sarah, et al.
(författare)
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Assessing Error Correlations in Remote Sensing-Based Estimates of Forest Attributes for Improved Composite Estimation
- 2018
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Ingår i: Remote Sensing. - : MDPI AG. - 2072-4292. ; 10
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Tidskriftsartikel (refereegranskat)abstract
- Today, non-expensive remote sensing (RS) data from different sensors and platforms can be obtained at short intervals and be used for assessing several kinds of forest characteristics at the level of plots, stands and landscapes. Methods such as composite estimation and data assimilation can be used for combining the different sources of information to obtain up-to-date and precise estimates of the characteristics of interest. In composite estimation a standard procedure is to assign weights to the different individual estimates inversely proportional to their variance. However, in case the estimates are correlated, the correlations must be considered in assigning weights or otherwise a composite estimator may be inefficient and its variance be underestimated. In this study we assessed the correlation of plot level estimates of forest characteristics from different RS datasets, between assessments using the same type of sensor as well as across different sensors. The RS data evaluated were SPOT-5 multispectral data, 3D airborne laser scanning data, and TanDEM-X interferometric radar data. Studies were made for plot level mean diameter, mean height, and growing stock volume. All data were acquired from a test site dominated by coniferous forest in southern Sweden. We found that the correlation between plot level estimates based on the same type of RS data were positive and strong, whereas the correlations between estimates using different sources of RS data were not as strong, and weaker for mean height than for mean diameter and volume. The implications of such correlations in composite estimation are demonstrated and it is discussed how correlations may affect results from data assimilation procedures.
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| 10. |
- Ekedahl, Henrik, et al.
(författare)
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Low-grade inflammation in survivors of childhood cancer and testicular cancer and its association with hypogonadism and metabolic risk factors
- 2022
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Ingår i: BMC Cancer. - : Springer Science and Business Media LLC. - 1471-2407. ; 22, s. 1-11
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Tidskriftsartikel (refereegranskat)abstract
- BACKGROUND: In childhood (CCS) and testicular cancer (TCS) survivors, low-grade inflammation may represent a link between testosterone deficiency (hypogonadism) and risk of metabolic syndrome. We aimed to study levels of inflammatory markers in CCS and TCS and the association with hypogonadism and future cardio-metabolic risk factors.METHODS: Serum levels of inflammatory markers and testosterone were analyzed in CCS (n = 90), and TCS (n = 64, median time from diagnosis: 20 and 2.0 years, respectively), and in controls (n = 44). Differences in levels between patients and controls were calculated using univariate analysis of variance. T-test and logistic regression were applied to compare levels of cardio-metabolic risk factors and odds ratio (OR) of hypogonadism and metabolic syndrome in low and high inflammatory marker groups after 4-12 years of follow up. Adjustment for age, smoking, and active cancer was made.RESULTS: TCS and CCS, as compared to controls, had 1.44 (95%CI 1.06-1.96) and 1.25 (95 CI 1.02-1.53) times higher levels of IL-8, respectively. High IL-6 levels were associated with hypogonadism at baseline (OR 2.83, 95%CI 1.25-6.43) and the association was stronger for high IL-6 combined with low IL-10 levels (OR 3.10, 95%CI 1.37-7.01). High IL-6 levels were also associated with higher BMI, waist circumference, insulin, and HbA1c at follow up. High TNF-α was associated with higher diastolic blood pressure. No individual inflammatory marker was significantly associated with risk of metabolic syndrome at follow up. High IL-6 combined with low IL-10 levels were associated with risk of metabolic syndrome (OR 3.83, 95%CI 1.07-13.75), however not statistically significantly after adjustment.CONCLUSION: TCS and CCS present with low-grade inflammation. High IL-6 levels were associated with hypogonadism and cardio-metabolic risk factors. Low IL-10 levels might reinforce the IL-6 mediated risk of developing metabolic syndrome.
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| 11. |
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| 12. |
- Glimelius, Bengt, et al.
(författare)
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A systematic overview of radiation therapy effects in rectal cancer.
- 2003
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Ingår i: Acta oncologica (Stockholm, Sweden). - : Informa UK Limited. - 0284-186X .- 1651-226X. ; 42, s. 476-
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Tidskriftsartikel (refereegranskat)abstract
- A systematic review of radiation therapy trials in several tumour types was performed by The Swedish Council of Technology Assessment in Health Care (SBU). The procedures for evaluation of the scientific literature are described separately (Acta Oncol 2003; 42: 357-365). This synthesis of the literature on radiation therapy for rectal cancer is based on data from 42 randomized trials and 3 meta-analyses. Moreover, data from 36 prospective studies, 7 retrospective studies and 17 other articles were used. A total of 131 scientific articles are included, involving 25 351 patients. The results were compared with those of a similar overview from 1996 including 15 042 patients. The conclusions reached can be summarized thus: The results after rectal cancer surgery have improved during the past decade. It is likely that local failure rates after 5 years of follow-up at hospitals adopting the TME-concept (TME = total mesorectal excision) have decreased from about 28% to 10-15%. Preoperative radiotherapy at biological effective doses above 30 Gy decreases the relative risk of a local failure by more than half (50-70%). Postoperative radiotherapy decreases the risk by 30-40% at doses that generally are higher than those used preoperatively. There is strong evidence that preoperative radiotherapy is more effective than postoperative. There is moderate evidence that preoperative radiotherapy significantly decreases the local failure rate (from 8% to 2% after 2 years) also with TME. There is strong evidence that preoperative radiotherapy improves survival (by about 10%). There is no evidence that postoperative radiotherapy improves survival. There is some indication that survival is prolonged when postoperative radiotherapy is combined with concomitant chemotherapy. Preoperative radiotherapy at adequate doses can be given with low acute toxicity. Higher, and unacceptable acute toxicity has been seen in some preoperative radiotherapy trials using suboptimal techniques. Postoperative radiotherapy can also be given with acceptable acute toxicity. The long-term consequences of radiotherapy appear to be limited with adequate radiation techniques, although they have been less extensively studied. Longer follow-up periods are needed before firm conclusions can be drawn. Peroperative radiotherapy, preferably preoperative since it is more effective, is routinely recommended for most patients with rectal cancer since it can substantially decrease the risk of a local failure and increases survival. In a primarily non-resectable tumour, preoperative radiotherapy can cause tumour regression allowing subsequent radical surgery. This therapy is routinely indicated. Whether radiochemotherapy is more efficient than radiotherapy alone is not clear, since the results of four small randomized trials are partly conflicting. Preoperative radiotherapy, frequently combined with chemotherapy, has been used to increase the chances of sphincter-preserving surgery in low-lying tumours. The literature is inconclusive with respect to how frequently this occurs. Radiotherapy frequently produces symptom relief in patients with rectal cancer not amendable to surgery.
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| 13. |
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| 14. |
- Hall, Per, 1954, et al.
(författare)
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Dissolved organic matter in abyssal sediments: Core recovery artifacts
- 2007
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Ingår i: Limnology and Oceanography. - 0024-3590. ; 52:1, s. 19-31
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Tidskriftsartikel (refereegranskat)abstract
- We report measurements of pore-water dissolved organic carbon (DOC), dissolved organic nitrogen, total dissolved carbohydrates, dissolved free monosaccharides, and ammonium in recovered deep-sea sediments from the Porcupine Abyssal Plain (PAP), Northeast Atlantic. There were distinct maxima close to the sediment–water interface of these constituents at all times of the year. The very high diffusive effluxes calculated from these porewater distributions were not compatible with simultaneous sediment trap measurements of particulate organic carbon, nitrogen, and carbohydrate fluxes toward the seafloor. Effluxes calculated from pore-water DOC distributions in recovered cores from another Atlantic deep-sea site, showing almost identical maxima as those at PAP, were more than an order of magnitude greater than simultaneous in situ chamber DOC flux measurements. We suggest that the dissolved organic matter maxima are predominantly artifacts induced by lysis of, or leakage from, mainly bacterial biomass resulting from decompression and/or warming during recovery of the sediment cores from the abyssal seafloor. Temperature elevation during core recovery from the abyss gives a N2 saturation of about 150%, and the combined effect of warming and decompression results in a CO2 saturation of about 135%, which together plausibly are associated with bubble formation creating cell bursting. Previous estimates of microbial biomass in abyssal sediments may be underestimates because of the difficulty of counting lysed bacterial cells. Since exoenzymes are inducible, previous measurements of their activities in recovered abyssal sediments may be overestimates.
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| 15. |
- Halvarsson Lundkvist, Agneta, et al.
(författare)
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Conditions Enabling Development in National Lean Programmes
- 2016
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Ingår i: Leadership & Organization Development Journal. - 0143-7739 .- 1472-5347.
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Tidskriftsartikel (refereegranskat)abstract
- Purpose – The purpose of this article is to identify work practices and activities at programme and local levels which constitute conditions that enable development in workplace development programmes (WPDPs). These are introduced by public agencies as change agents that provide the resources for local organizational change. Design/methodology/approach – The study constituted separate interactive research projects in three WPDPs conducted through a total of 256 interviews and documentation from meetings. The findings were compared in a qualitative content analysis. Findings – The outcomes of development processes in the programmes and local Lean implementation are enabled by the interplay of three conditions: a) organized learning activities with the stakeholders involved, b) key stakeholders’ active ownership and c) support for employee participation. The three conditions thus support programme management in leading the programme development processes. Research limitations/implications - The findings operationalize broad theoretical concepts. However, the research projects involved were not originally designed for this comparative study, which created some difficulties when performing the analysis. The longitudinal design and vast amount of data partly compensate for this. Practical implications – This knowledge will be helpful in the formation of new WPDPs that support local organizational development and for those who lead such processes. Originality/value – The novelty of the findings is the operationalization of broad theoretical concepts, such as the conditions that support development programmes. Moreover, this article shows a set of work practices and activities that management may be used in organizing WPDPs.
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| 16. |
- Hjelm, Barbara, et al.
(författare)
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Exploring epitopes of antibodies toward the human tryptophanyl-tRNA synthetase
- 2010
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Ingår i: NEW BIOTECHNOL. - : Elsevier BV. - 1871-6784. ; 27:2, s. 129-137
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Tidskriftsartikel (refereegranskat)abstract
- There is a need to characterize the epitopes of affinity reagents to develop high quality affinity reagents for research, diagnostics and therapy. Here, we describe the analysis of epitopes of antibodies generated toward human tryptophanyl-tRNA synthetase (WARS) using both combinatorial bacterial display and suspension bead array. The bacterial display revealed that the polyclonal antibody binds to three separate epitopes and peptide scanning using 15-mers revealed binding to a 13 amino acid consensus sequence (ELINRIERATGQR). A mouse monoclonal antibody was generated and the mapping approach revealed binding toward a slightly shifted position of the same epitope. Structural analysis showed that the antibodies bind to a-helical regions on the surface of the target protein. An alanine-scanning experiment showed binding to four specific residues. The implications for the systematic analysis of antibody epitopes on the basis of these results are discussed.
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| 17. |
- Högström, Ebba, 1961-
(författare)
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Kalejdoskopiska rum : Diskurs, materialitet och praktik i den decentraliserade psykiatriska vården
- 2012
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Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- During the period 1967-1995, Swedish mental healthcare underwent a complete re-organisation, starting with county councils taking over responsibility for mental healthcare from the state. Asylums were then phased out and mental health care moved closer to patients. The Mental Health Reform of 1995 completed this decentralisation and put the emphasis on an independent and integrated life as a citizen in society and the idea of a dwelling of one’s own. This thesis describes and analyses spatial aspects of decentralised mental healthcare in Sweden, focusing on the decentralisation discourse regarding organisation, localisation, patient care and working methods behind decentralisation and its spatial performance. A case study of decentralised mental healthcare in Nacka, a Stockholm suburb, between 1958-1999 examines in particular the emerging decentralisation discourse 1958-1973, The Nacka Project 1974-1980 (one of the first examples of community care in Sweden), psychiatry in Nacka 1980-1994 and the official report Welfare and Freedom of Choice from 1995. The methods used include studies of documents, interviews, visual and architectural drawing analysis. The theoretical point of departure for the analysis is a post-structural heterogeneous concept of space where spatial materiality and discursiveness are looked upon as intertwined. The result shows that the re-organisation of mental healthcare brought about a substantial spatial transformation. Normalisation of patients’ lives involved integration into society and support for independent living. The local environment was the main trope for the early stage of decentralised mental healthcare, but the notion of a dwelling of one’s own became the important trajectory to an independent life after 1995. The idea of the patient is challenged by the independence discourse, which could be said to contain an idea of the ‘non-patient’. Overall, it can be concluded that spatial organisations of the built environment are never value-free or neutral. They reflect, enable and constrain power relations in a society and material space can contribute to the power of one group at the expense of another. Furthermore, the results of the spatialities, or the meanings, cannot be predicted. It is therefore crucial to distinguish power in all its configurations and scales and to keep negotiations alive, especially within the field of mental healthcare, but also in the care sector as a whole and in other societal institutions where policies buildings and built environment interact with user practices. This kaleidoscopic perspective can be used for examining complexities in the past and present and for encouraging future potentialities in the process of making/enacting spatial relations.
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| 18. |
- Ioannidis, Konstantinos, 1976-
(författare)
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Designing the Edge : An Inquiry into the Psychospatial Nature of Meaning in the Architecture of the Urban Waterfront
- 2011
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Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- The initial goal of this effort is to develop a discussion on urban design process and thinking that acknowledges the needs of places with meaning in the design of the urban waterfront. The thesis addresses the fact that the problematic of the coastal formulation is intricate, comprising not only aspects related to the spatial organization and design of its domain but also shared properties originated by the presence and movement of the perceiving subject in the area. In this framework, the research attempts to provide an understanding of the main relationships that the subject cultivates inside the coastal space and to offer a broader spatial reading of its narrative function. On the hypothesis that this function is susceptible of interpretation, the thesis develops an interest in examining the effects of the psychospatial nature of meaning on the design and experience of the urban edge, for to interpret a narrative spatial construct is to specify its meaning. To explore the issue of waterfront places that speak of the subject, the research conceives the coastal space as a field of mediated parameters that pertain to three crucial operational premises: the symbolic function of the urban space near the water, the meaning behind the coastal form, and the engagement of the perceiving subject in the conscious or reflexive appropriation of the waterfront setting. These premises, traced as psychophysiological spaces, determine the intermediary, the integrative, and the expressive discourses for the development of places with meaning near the water. Through them, the thesis attempts a reading of the coastal domain based upon the material interpretation of the meanings and messages associated with the immediate experience of the onset of water‐born notions, concepts, and images. Writing about the dialectics between the psychospatial inquiry and the spatial experience of the edge, this thesis suggests that, contrary to the established preconception, the psychology of human‐edge relations submits the perceiving subject to the conception of the coastal form and shape.
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| 19. |
- Karlsson, Henrik, et al.
(författare)
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Termisk komfort i lågenergihus och passivhus
- 2012
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Rapport (refereegranskat)abstract
- Projektets målsättning är att bedöma hur avsaknaden av radiatorer och golvvärme påverkar den termiska komforten i lågenergihus och passivhus. Målsättningen är också att visa hur fönsterutformning samt valet av golvmaterial påverkar den upplevda termiska komforten i byggnaden.
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| 20. |
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| 21. |
- Karlsson, Reine, et al.
(författare)
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Flervetenskaplig ljusforskning
- 2011
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Rapport (populärvet., debatt m.m.)abstract
- Den här skriften handlar om ljus och ljusets betydelse för oss människor. Tolv forskare med vitt skilda bakgrunder samlas och diskuterar frågor som skrider över gränserna. Vad är ljus egentligen? Hur kan vi förstå ljus? Hur påverkar det oss? På vilket sätt kan vi använda oss av det? Vilka tekniska möjligheter har vi att skapa och styra olika sorters ljus? Vilka möjligheter ger det oss inför framtiden? I centrum för dialogen står samarbete och nytänkande. Här ges en inblick i perspektiv på ljus från områden som biologi, teknik, material, fysik, estetik, filosofi, medicin och psykologi, vilka sammantaget, på ett åskådligt och lättbegripligt sätt, presenterar den flervetenskapliga ljusrelaterade kompetensen i Lund. Gruppen av experter hoppas att de frågor som ställs och de svar som ges i den här skriften ska inspirera och leda vidare inom fältet ljusforskning. Tillsammans med Pufendorfinstitutet, genom vilket expertgruppens initiativ getts möjlighet att växa och utvecklas, vill de med andra ord att sätta ljuset på...just ”ljuset”!
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| 22. |
- Kronqvist, Nina, et al.
(författare)
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A novel affinity protein selection system based on staphylococcal cell surface display and flow cytometry
- 2008
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Ingår i: Protein Engineering Design & Selection. - : Oxford University Press (OUP). - 1741-0126 .- 1741-0134. ; 21:4, s. 247-255
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Tidskriftsartikel (refereegranskat)abstract
- Here we describe the first reported use of a Gram-positive bacterial system for the selection of affinity proteins from large combinatorial libraries displayed on the surface of Staphylococcus carnosus. An affibody library of 3 x 109 variants, based on a 58 residue domain from staphylococcal protein A, was pre-enriched for binding to human tumor necrosis factor-alpha (TNF-alpha) using one cycle of phage display and thereafter transferred to the staphylococcal host (106 variants). The staphylococcal-displayed library was subjected to three rounds of flow-cytometric sorting, and the selected clones were screened and ranked by on-cell analysis for binding to TNF-alpha and further characterized using biosensor analysis and circular dichroism spectroscopy. The successful sorting yielded three different high-affinity binders (ranging from 95 pM to 2.2 nM) and constitutes the first selection of a novel affinity protein using Gram-positive bacterial display. The method combines the simplicity of working with a bacterial host with the advantages of displaying recombinant proteins on robust Gram-positive bacteria as well as using powerful flow cytometry in the selection and characterization process.
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| 23. |
- Kronqvist, Nina, et al.
(författare)
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Simplified characterization through site-specific protease-mediated release of affinity proteins selected by staphylococcal display
- 2008
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Ingår i: FEMS Microbiology Letters. - : Oxford University Press (OUP). - 0378-1097 .- 1574-6968. ; 278:1, s. 128-136
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Tidskriftsartikel (refereegranskat)abstract
- The production of candidate affinity proteins in a soluble form, for downstream characterization, is often a time-consuming step in combinatorial protein engineering methods. Here, a novel approach for efficient production of candidate clones is described based on direct cleavage of the affinity protein from the surface of Staphylococcus carnosus, followed by affinity purification. To find a suitable strategy, three new fusion protein constructs were created, introducing a protease site for specific cleavage and purification tags for affinity chromatography purifications into the staphylococcal display vector. The three modified strains were evaluated in terms of transformation frequency, surface expression level and protease cleavage efficiency. A protocol for efficient affinity purification of protease-released affinity proteins using the introduced fusion-tags was successfully used, and the functionality of protease-treated and purified proteins was verified in a biosensor assay. To evaluate the devised method, a previously selected HER2-specific affibody was produced applying the new principle and was used to analyze HER2 expression on human breast cancer cells.
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| 24. |
- Leach, Maddie, 1970, et al.
(författare)
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Fountains Failures Futures: The afterlives of public art
- 2023
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Ingår i: Fountains Failures Futures: The afterlives of public art. - Lund : Skissernas Museum - Museum of Artistic Process and Public Art.
-
Annan publikation (övrigt vetenskapligt/konstnärligt)abstract
- Research and discussion about public art frequently focuses on conditions of emergence and production for public works. Fountains Failures Futures starts at the other end – thinking about the afterlives of public art in relation to processes of decline, decay, acts of reparation and re-imagination, transformation, and change. We are interested in questions of sustainability,custodianship, and whether “failed” public artworks can be revitalised and thought anew. What are the implications for their authorship and ownership, and what challenges does this present for commissioners, artists, architects, and urban planners?How can producers, owners and custodians of public artworks, and the communities living with these works, anticipate and accommodate complex afterlives of public art?
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| 25. |
- Lehtio, J., et al.
(författare)
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Directed immobilization of recombinant staphylococci on cotton fibers by functional display of a fungal cellulose-binding domain
- 2001
-
Ingår i: FEMS Microbiology Letters. - 0378-1097 .- 1574-6968. ; 195:2, s. 197-204
-
Tidskriftsartikel (refereegranskat)abstract
- The immobilization of recombinant staphylococci onto cellulose fibers through surface display of a fungal cellulose-binding domain (CBD) was investigated. Chimeric proteins containing the CBD from Trichoderma reesei cellulase Cel6A were found to be correctly targeted to the cell wall of Staphylococcus carnosus cells. since full-length proteins could be extracted and affinity-purified. Furthermore. surface accessibility of the CBD was verified using a monoclonal antibody and functionality in terms of cellulose-binding was demonstrated in two different assays in which recombinant staphylococci were found to efficiently bind to cotton fibers. The implications of this strategy of directed immobilization Tor the generation of whole-cell microbial tools Fur different applications will be discussed.
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| 26. |
- Linde, Peter, 1956-, et al.
(författare)
-
Otakt 2006 - 2009 : I otakt bland toner och ord på internet
- 2021
-
Annan publikation (populärvet., debatt m.m.)abstract
- Otakt.se startades tidigt 2006. Sajten var en tummelplats för fem griniga gubbar som ville ha möjligheten att fritt tycka om gammal och ny musik.Tanken var att skriva CD recensioner inom de musikaliska genrer som intresserade oss: pop, rock, soul, blues, visa, jazz, hårdrock, grunge, indie, emo, country, lo-fi, americana, metal och världsmusik.Förutom recensioner fanns det gott om plats för olika listor (årets bästa... etc.) och texter som (kanske) hade något med musik att göra. Allt gjordes av lust, och bara sådant vi själva ville skriva om dök upp på sajten.2009 tog lusten slut och vi avvecklade otakt.se men nu drygt tio år senare vill vi ändå göra texterna åtkomliga dels av nostalgiska skäl och för att vi tror att läsare och musikälskare fortfarande kan hitta inspel här som kan leda till spännande musikaliska möten men kanske framför allt för att hedra Björn Mogreen, som var den drivande i projektet och som la ifrån sig pennan och slutade att lyssna på musik våren 2021. Han var en sann otaktsbroder och vi saknar honom mycket.
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| 27. |
- Lindgren, Nils, et al.
(författare)
-
Improved Prediction of Forest Variables Using Data Assimilation of Interferometric Synthetic Aperture Radar Data
- 2017
-
Ingår i: Canadian Journal of Remote Sensing. - : Informa UK Limited. - 0703-8992 .- 1712-7971. ; 43, s. 374-383
-
Tidskriftsartikel (refereegranskat)abstract
- The statistical framework of data assimilation provides methods for utilizing new data for obtaining up-to-date forest data: existing forest data are forecasted and combined with each new remote sensing data set. This new paradigm for updating forest database, well known from other fields of study, will provide a framework for utilizing all available remote sensing data in proportion to their quality to improve prediction. It also solves the problem that not all remote sensing data sets provide information for the entire area of interest, since areas with no remote sensing data can be forecasted until new remote sensing data become available. In this study, extended Kalman filtering was used for assimilating data from 19 TanDEM-X InSAR images on 137 sample plots, each of 10-meter radius at a test site in southern Sweden over a period of 4 years. At almost all time points data assimilation resulted in predictions closer to the reference value than predictions based on data from that single time point. For the study variables Lorey's mean height, basal area, and stem volume, the median reduction in root mean square error was 0.4 m, 0.9 m(2)/ha, and 15.3 m(3)/ha (2, 3, and 6 percentage points), respectively.
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| 28. |
- Lindvall, Rebecka, et al.
(författare)
-
Performance and wear mechanisms of PCD and pcBN cutting tools during machining titanium alloy Ti6Al4V
- 2020
-
Ingår i: Wear. - : Elsevier BV. - 0043-1648. ; 454-455
-
Tidskriftsartikel (refereegranskat)abstract
- The need for increased productivity in difficult-to-machine titanium alloys has pushed manufacturers to examine the potential of ultrahard cutting tool materials such as polycrystalline diamond and polycrystalline cubic boron nitride as alternative solutions to conventional cemented carbide tools. This study examines the performance of such advanced tool materials in high speed finishing machining of Ti6Al4V, with attained PCD superiority compared to pcBN. Wear mechanisms are experimentally investigated based on in-depth microscopic analyses using techniques such as scanning electron microscopy, transmission electron microscopy, electron diffraction and X-ray energy-dispersive spectroscopy. Main wear morphologies were flank wear and cratering in both tool materials. Flank fracture caused by micro-cracking was an additional deterioration mechanism of pcBN tooling. Diamond burn-out, likely in combination with graphitization of diamond, was causing channel like wear morphology. The PCD wear mechanism was diffusion dissolution of carbon in Ti6Al4V. (Ti,V)C diffusional barrier or Tool Protection Layer (TPL) was formed due to reaction of workpiece and tool materials in presence of cobalt. Controlled grain size and increased cobalt content resulted in higher performance as protective caps of (Ti,V)C merged to form a continuous TPL. Similarly for pcBN, (Ti,V)B2 and (Ti,V,Cr)B2 reaction products acted as TPLs which reduced the tool deterioration rate.
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| 29. |
- Löfblom, John, et al.
(författare)
-
Evaluation of staphylococcal cell surface display and flow cytometry for postselectional characterization of affinity proteins in combinatorial protein engineering applications
- 2007
-
Ingår i: Applied and Environmental Microbiology. - 0099-2240 .- 1098-5336. ; 73:21, s. 6714-6721
-
Tidskriftsartikel (refereegranskat)abstract
- For efficient generation of high-affinity protein-based binding molecules, fast and reliable downstream characterization platforms are needed. In this work, we have explored the use of staphylococcal cell surface display together with How cytometry for affinity characterization of candidate affibody molecules directly on the cell surface. A model system comprising three closely related affibody molecules with different affinities for immunoglobulin G and an albumin binding domain with affinity for human serum albumin was used to investigate advantages and differences compared to biosensor technology in a side-by-side manner. Equilibrium dissociation constant (K-D) determinations as well as dissociation rate analysis were performed using both methods, and the results show that the on-cell determinations give both KD and dissociation rate values in a very fast and reproducible manner and that the relative affinities are very similar to the biosensor results. Interestingly, the results also show that there are differences between the absolute affinities determined with the two different technologies, and possible explanations for this are discussed. This work demonstrates the advantages of cell surface display for directed evolution of affinity proteins in terms of fast postselectional, on-cell characterization of candidate clones without the need for subcloning and subsequent protein expression and purification but also demonstrates that it is important to be aware that absolute affinities determined using different methods often vary substantially and that such comparisons therefore could be difficult.
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| 30. |
- Löfblom, John, et al.
(författare)
-
Fine affinity discrimination by normalized fluorescence activated cell sorting in staphylococcal surface display
- 2005
-
Ingår i: FEMS Microbiology Letters. - : Oxford University Press (OUP). - 0378-1097 .- 1574-6968. ; 248:2, s. 189-198
-
Tidskriftsartikel (refereegranskat)abstract
- We have investigated a staphylococcal surface display system for its potential future use as a protein library display system ill combinatorial biochemistry. Efficient affinity-based selections require a system capable of fine affinity discrimination of closely related binders to minimize the loss of potentially improved variants. In this Study, a significant breakthrough was achieved to avoid biases due to potential cell-to-cell variations in surface expression levels, since it was found that a generic protein tag, present within the displayed recombinant surface proteins on the cells, could be successfully employed to obtain normalization of the target-binding signal. Four mutated variants of a staphylococcal protein A domain with different affinity to human IgG were successfully expressed on the surface of recombinant Staphylococcus carnosus cells. The system was evaluated for affinity-based cell sorting experiments, where cell-displayed protein A domains with an 8-fold difference in target affinity were mixed at a ratio of 1: 1000 and sorted using FACS. Enrichment factors around 140-fold were obtained from a single round of sorting under normal library sorting conditions when the top 0.1% fraction having the highest antigen binding to Surface expression level ratio was sorted. The results demonstrate that the system would have a potential as a selection system in protein library display applications, and the normalization strategy should indeed make it possible to achieve fine affinity discriminations in future library selections. (c) 2005 Federation of European Microbiological Societies.
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| 31. |
- Löfblom, John, et al.
(författare)
-
Optimization of electroporation-mediated transformation : Staphylococcus carnosus as model organism
- 2007
-
Ingår i: Journal of Applied Microbiology. - : Oxford University Press (OUP). - 1364-5072 .- 1365-2672. ; 102:3, s. 736-747
-
Tidskriftsartikel (refereegranskat)abstract
- The study was conducted with an aim to optimize the transformation efficiency of the Gram-positive bacterium Staphylococcus carnosus to a level that would enable the creation of cell surface displayed combinatorial protein libraries. Methods and Results: We have thoroughly investigated a number of different parameters for: (i) the preparation of electrocompetent cells; (ii) the treatment of cells before electroporation; (iii) the electroporation step itself; and (iv) improved recovery of transformed cells. Furthermore, a method for heat-induced inactivation of the host cell restriction system was devised to allow efficient transformation of the staphylococci with DNA prepared from other species, such as Escherichia coli. Previously described protocols for S. carnosus, giving transformation frequencies of approximately 10(2) transformants per transformation could be improved to reproducible procedures giving around 10(6) transformants for a single electroporation event, using plasmid DNA prepared from either S. carnosus or E. coli. The transformed staphylococcal cells were analysed using flow cytometry to verify that the entire cell population retained the introduced plasmid DNA and expressed the recombinant protein in a functional form on the cell surface at the same level as the positive control population. Conclusions: The results demonstrate that the transformation frequency for S. carnosus could be dramatically increased through optimization of the entire electroporation process, and that the restriction barrier for interspecies DNA transfer, could be inactivated by heat treatment of the cells prior to electroporation. Significance and Impact of the Study: The generation of large combinatorial protein libraries, displayed on the surface of S. carnosus can be envisioned in the near future, thus dramatically improving the selection compared with the traditional biopanning procedure used in phage display.
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| 32. |
- Löfblom, John, 1977-
(författare)
-
Staphylococcal surface display for protein engineering and characterization
- 2007
-
Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- Even though our understanding of mechanisms such as protein folding and molecular recognition is relatively poor, antibodies and alternative affinity proteins with entirely novel functions are today generated in a routine manner. The reason for this success is an engineering approach generally known as directed evolution. Directed evolution has provided researchers with a tool for circumventing our limited knowledge and hence the possibility to create novel molecules that by no means could have been designed today. The approach is based on construction of high-complexity combinatorial libraries from which protein variants with desired properties can be selected. Engineered proteins are already indispensable tools in nearly all areas of life science and the recent advent of mainly monoclonal antibodies as therapeutic agents has directed even more attention to the field of combinatorial protein engineering. In this thesis, I present the underlying research efforts of six original papers. The overall objective of the studies has been to develop and investigate a new staphylococcal surface display method for protein engineering and protein characterization. The technology is based on display of recombinant proteins on surface of the Gram-positive bacteria Staphylococcus carnosus. In two initial studies, two key issues were addressed in order to improve the protein engineering method in regard to affinity discrimination ability and transformation efficiency. The successful results enabled investigation of the staphylococcal display system for de novo generation of affibody molecules from large combinatorial libraries. In this study, a high-complexity protein library was for the first time displayed on surface of Gram-positive bacteria and by means of fluorescence-activated cell sorting, specific affinity proteins for tumor necrosis factor-alpha were isolated. Moreover, in following papers, the staphylococcal display method was further improved and investigated for affinity determination, soluble protein production and epitope mapping purposes in order to facilitate downstream characterizations of generated affinity proteins. Taken together, in these studies we have demonstrated that the staphylococcal display system is a powerful alternative to existing technologies for protein engineering and protein characterization.
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| 33. |
- Nobel, Andreas, 1966-
(författare)
-
Dimmer på Upplysningen : text, form och formgivning
- 2014
-
Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- The main part of the dissertation is a text. The other is an exhibition mainly presenting a bow-lathe and some furniture designed and produced in that bow lathe. Interior- and furniture design are disciplines in which sensory qualities are important. Within these disciplines there are well established methods and languages for developing these qualities. However, such development has rarely been achieved through the medium of text. Sensory qualities tend to be ignored in highly textualized knowledge environments. Within education textualized knowledge is often valued higher than forms of knowledge developed through other medias than text. This situation has led to a dichotomized perception of knowledge where textualized knowledge attained through writing and conceptualizing, is valued higher than knowledge developed through physical work. The thesis argues that this hierarchical view on different forms of knowledge also has an influence on the practical profession of the designer, manifest in the paradoxical situation where the form aspects of design is neglected and over shadowed by various forms of textualized knowledge. The central research question posed in the text part is: Which adverse effects might an increasing emphasis on textualized theory have on the design practices? The questions are highlighted from perspectives such as; epistemology, tradition, history and power.The central research question in the exhibition part examines if any possible negative effects on design resulting from the above mentioned scenario, may be prevented through engaging in a highly physical and non-conceptual design- process? The purpose of the exhibition part is to introduce methods and design that may provide the impetus for further development in the fields of design. The bow lathe is presented as an example of a productive tool for the development of relevant contemporary design.
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| 34. |
- Nordegren, Annica, 1954-
(författare)
-
Design–theoretical and empirical, A research project inn design theory including the devolpment of a prototype for a symbol system that represents tastes of wine
- 2004
-
Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- The thesis presents a research project in graphic design including the development of a symbol system that represents tastes of wine as consumer information. The thesis has three main chapters; design, theory and empirical foundation. The design chapter clarifies the design concept and its evolution from a traditional standpoint to its present state in the international area of design research as design theory. The theoretical chapter presents a map of concepts in order to explain problems and practical solutions in the design process. The concepts are based on the philosophical ideas of Ernst Cassirer and Ludwig Wittgenstein, and concern interpretation of sense information, symbols and signs, picture and image. The design of the symbol system concerns the experience of taste, as a translation from words to pictures. The empirical chapter comprehends both a description of the design process and of the empirical research. The method used is prototyping with a reference group of branch experts. The empirical research has evaluated the possibility to interpret, learn, use and understand the symbol system. The method used is a survey with questionnaires to a group of wine consumers
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| 35. |
- Olsson, Mike, et al.
(författare)
-
FE simulation and experimental verification of side-flow and burr formation in machining of oxygen-free copper
- 2018
-
Ingår i: Procedia CIRP. - : Elsevier BV. - 2212-8271. ; 72, s. 1427-1432
-
Konferensbidrag (refereegranskat)abstract
- This article considers the influence of cutting data on burr formation when machining oxygen-free copper in a longitudinal turning operation. Burr formation and side-flow frequently occur in a cutting process, resulting in highly undesirable poor surface integrity and reduced dimensional accuracy of the machined components. Finite Element (FE) simulations of a cutting process were used to simulate radial burr formation for variable cutting depth, feed and major cutting edge angle. The results from FE simulations are compared with the experiments of longitudinal turning. Decrease of depth of cut and major cutting edge angle result in reduced burr formation. Additionally, high speed filming performed during the machining revealed a five-stage cycle of burr formation.
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| 36. |
- Olsson, Mike, et al.
(författare)
-
Study of wear mechanisms of cemented carbide tools during machining of single-phase niobium
- 2020
-
Ingår i: Wear. - : Elsevier BV. - 0043-1648 .- 1873-2577. ; 450-451
-
Tidskriftsartikel (refereegranskat)abstract
- In particle accelerator facilities, single-phase niobium is used in superconducting accelerator components. Machining induced surface quality of such components is strongly connected to the functionality of accelerators. In this study, tool wear development and its influence on the surface quality of Nb workpiece have been investigated in longitudinal turning. Uncoated cemented carbide cutting tools were used under finishing conditions (cutting speed vc = 300 m/min and feed f = 0.05 mm/rev) up to the wear criterion of VBmax = 300 μm. A detailed analysis of wear mechanisms of the cutting tool was conducted with help of high resolution electron microscopy (SEM and TEM). Further, the results obtained were correlated with controlled diffusion couples experiments under high pressure – high temperature conditions (2 GPa and 1000 °C). Diffusion of carbon from WC and formation of NbC was found to occur on the niobium – cemented carbide interface. Electron microscopy of the worn tools reports identical mechanisms of diffusion and chemical interaction which lead to rapid flank wear, yet formation of NbC on the rake completely inhibits tool degradation and thus acts as tool protection layer.
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| 37. |
- Olsson, Mike, et al.
(författare)
-
Surface roughness and sub-surface deformation measurements in machining of niobium
- 2018
-
Ingår i: Procedia CIRP : 4th CIRP Conference on Surface Integrity (CSI 2018) - 4th CIRP Conference on Surface Integrity (CSI 2018). - : Elsevier BV. - 2212-8271. ; 71, s. 413-417
-
Konferensbidrag (refereegranskat)abstract
- Single-phase niobium is used in superconducting accelerator components in particle accelerating facilities. Functionality of such accelerator components is placing high demands on the surface quality. Single-phase niobium, as opposed to niobium alloys, is a highly ductile metal, which in a cutting process normally leads to such surface defects as adhesion, built-up edges, side flow, and burr formation. In this study, six different tool materials: uncoated and two types of coated cemented carbide, PCBN, PCD and single-crystal diamond have been tested in longitudinal turning of niobium at different cutting parameters, in order to achieve the target value of Ra = 0.8 μm. Experimental studies involved finish machining with varied feed and cutting speed, while depth of cut was kept at ap = 0.25 mm. Apart from the resulting surface finish Ra and cutting forces, the following surface quality parameters were analysed: side-flow, adhesion, burr formation and sub-surface deformation. Only two of the tool materials, PCD and nanolaminate TiAlN/TiSiN coated cemented carbide, have shown acceptable surface integrity results.
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| 38. |
- Persson, Henrik, et al.
(författare)
-
Characterizing Uncertainty in Forest Remote Sensing Studies
- 2020
-
Ingår i: Remote Sensing. - : MDPI AG. - 2072-4292. ; 12
-
Tidskriftsartikel (refereegranskat)abstract
- This discussion paper addresses (1) the challenge of concisely reporting uncertainties in forest remote sensing (RS) studies, primarily conducted at plot and stand level, and (2) the influence of reference data errors and how corrections for such errors can be made. Different common ways of reporting uncertainties are discussed, and a parametric error model is proposed as a core part of a comprehensive approach for reporting uncertainties (compared to, e.g., conventional reporting of root mean square error (RMSE)). The importance of handling reference data errors is currently increasing since estimates derived from RS data are becoming increasingly accurate; in extreme cases the accuracies of RS- and field-based estimates are of equal magnitude and there is a risk that reported RS accuracies are severely misjudged due to inclusion of errors from the field reference data. Novel methods for correcting for some types of reference data errors are proposed, both for the conventional RMSE uncertainty metric and for the case when a parametric error model is applied. The theoretical framework proposed in this paper is demonstrated using real data from a typical RS study where airborne laser scanning and synthetic aperture radar (SAR) data are applied for estimating biomass at the level of forest stands. With the proposed correction method, the RMSE for the RS-based estimates from laser scanning was reduced from 50.5 to 49.5 tons/ha when errors in the field references were properly accounted for. The RMSE for the estimates from SAR data was reduced from 28.5 to 26.1 tons/ha.
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| 39. |
- Persson, Henrik, et al.
(författare)
-
Experimental and Numerical Investigation of Burr Formation in Intermittent Turning of AISI 4140
- 2017
-
Ingår i: 16th CIRP Conference on Modelling of Machining Operations (16th CIRP CMMO). - : Elsevier BV. - 2212-8271. - 9781510842762 ; 58, s. 37-42
-
Konferensbidrag (refereegranskat)abstract
- Burr formation is a problematic aspect in the machining of ductile metals. Understanding the formation mechanism and being able to model the plastic workpiece behavior during tool exits is key to burr minimization. A 3D finite element model is used to simulate burr formation during the exit phase of intermittent turning of AISI 4140, using a nose radiused insert. The model is validated by experimental results of dynamic force measurement and analysis of the burrs produced. The exit burr height obtained via simulation closely corresponds to the experimentally obtained values.
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| 40. |
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| 41. |
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| 42. |
- Persson, Henrik, et al.
(författare)
-
Modelling tool life in high speed machining of AD730
- 2018
-
Ingår i: Procedia Manufacturing. - : Elsevier BV. - 2351-9789. ; 25, s. 316-321, s. 316-321
-
Tidskriftsartikel (refereegranskat)abstract
- AD730® is a new type of nickel-based superalloy, developed by Aubert & Duval, with high temperature properties exceeding conventional superalloys such as Alloy 718. This paper presents the tool life model for this superalloy for the purpose of process optimization and manufacturing cost estimation. The tool life modelling is based on comprehensive machining tests, using the Colding model. The cutting tool material used is CBN 170, a grade specially designed and optimized for machining of nickel-based superalloys.
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| 43. |
- Persson, Henrik, et al.
(författare)
-
Performance and wear mechanisms of different PcBN tools when machining superalloy AD730
- 2022
-
Ingår i: Ceramics International. - : Elsevier BV. - 0272-8842. ; 48:16, s. 22733-22742
-
Tidskriftsartikel (refereegranskat)abstract
- Nickel-based superalloys are known to be difficult to machine. These alloys are generally machined with cemented carbide tools under low productivity process conditions. Superhard polycrystalline cubic boron nitride (PcBN) tooling offers the possibility of increased production rates, however, a thorough understanding of the material performance and degradation is required before a transition from carbide tools occurs. This study investigated the performance and wear mechanism of three different PcBN tools with low (50 vol. %), medium (65 vol. %), and high (90 vol. %) cBN content, when turning AD730, a new Ni–Co-based superalloy. Due to the novelty of the material, little research has been done on its machinability with carbide and even less with PcBN tooling. The results showed the high-cBN grade was the best performer in terms of machining time. However, an undesirable wear morphology is generated, leading to excessively high cutting forces. Both the medium-cBN and low-cBN showed promise with stable rake and flank wear, but with notching as a drawback. Degradation analysis of medium-cBN grade revealed diffusional dissolution of cBN and formation of a reaction layer atop the cBN grains. The reaction layer, which acts as a diffusional barrier, consisted of three sub-layers: (1) Al2O3, (2) (Ti, Nb, Zr)N, and (3) (Ti, Cr, Nb, Zr)N. The low-cBN grade also showed a reaction layer; however, the smaller cBN grain size of this grade did not allow the layer to sufficiently stabilize.
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| 44. |
- Persson, Henrik, et al.
(författare)
-
Quantify and account for field reference errors in forest remote sensing studies
- 2022
-
Ingår i: Remote Sensing of Environment. - : Elsevier. - 0034-4257 .- 1879-0704. ; 283
-
Tidskriftsartikel (refereegranskat)abstract
- Field inventoried data are often used as references (ground truth) in forest remote sensing studies. However, the reference values are affected by various kinds of errors, which tend to make the reported accuracies of the remote sensing-based predictions worse than they are. The more accurate the remote sensing techniques are becoming, the more pronounced this problem will be. This paper addresses the impact of uncertainties in field reference data due to measurement errors, model errors, and position errors when evaluating the accuracy of biomass predictions from airborne laser scanning at plot level. We present novel theoretical analysis methods that take the interactions of the error sources into account. Further, an error characterization model (ECM) is used to describe the error structure of the remote sensing-based predictions, and we show how the parameters of the ECM can be adjusted when field references contain errors. We also show how root mean square error (RMSE) estimates can be adjusted. Based on data from Scandinavian forests, we conclude that the field reference errors have an impact on the remote sensing-based predictions. By accounting for these errors the RMSE of the remote sensing-based predictions was reduced by 6–18%. The most influential sources of error in the field references were found to be the residual errors of the allometric biomass model and the field plot position errors. Together, these two sources accounted for 97% of the variance while measurement errors and biomass model parameter uncertainties were negligible in our study.
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| 45. |
- Persson, Henrik, et al.
(författare)
-
Wear mechanisms of PcBN tools when machining AISI 316L
- 2021
-
Ingår i: Ceramics International. - : Elsevier BV. - 0272-8842. ; 47:22, s. 31894-31906
-
Tidskriftsartikel (refereegranskat)abstract
- This paper investigates the machinability of AISI 316L stainless steel without and with NMI treatment, when machined with three different PcBN tool grades: low cBN content (50% vol.), medium (65% vol.) and high (90% vol.). The NMI treatment consisted of resulfurized and Ca-treated steels with two different Si/Al ratio. The tool materials were evaluated in terms of tool life, showing the best performance for the medium cBN content grade. Results showed that the crater wear has a more significant effect on the tool performance than the standard wear criteria of maximum flank wear. The worn tools were examined using scanning electron microscopy (SEM), focused ion beam (FIB) and transmission electron microscopy (TEM). In most cases, the crater exhibited three distinct regions from the edge to the end of the contact zone: the plateau, middle crater, and the upper region. Plateau and crater region displayed tool protective layer (TPL), consisting mostly of Al, Si, Mg and Ca-oxides; and some dimples in the tool from preferential wear of cBN grains. In the upper region of the best performing material, a ~1.5 μm thick TPL of mostly MnCr2O4 spinel was found, a result of the transfer of Mn- and Cr-rich oxide NMIs found in this material or oxidation of workpiece material, during the machining process.
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| 46. |
- Reinstrup, Peter, et al.
(författare)
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Intracerebral microdialysis in clinical practice: baseline values for chemical markers during wakefulness, anesthesia, and neurosurgery
- 2000
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Ingår i: Neurosurgery. - : Ovid Technologies (Wolters Kluwer Health). - 0148-396X .- 1524-4040. ; 47:3, s. 701-710
-
Tidskriftsartikel (refereegranskat)abstract
- OBJECTIVE: The study was undertaken to measure baseline values for chemical markers in human subjects during wakefulness, anesthesia, and neurosurgery, using intracerebral microdialysis. METHODS: Microdialysis catheters were inserted into normal posterior frontal cerebral cortex in nine patients who were undergoing surgery to treat benign lesions of the posterior fossa. The perfusion rate was 1.0 microl/min during anesthesia/neurosurgery and the early postoperative course and 0.3 microl/min during the later course. Bedside biochemical analyses of glucose, pyruvate, lactate, glycerol, glutamate, and urea were performed before, during, and after neurosurgery. After the bedside analyses, all samples were frozen for subsequent high-performance liquid chromatographic analyses of amino acids. RESULTS: The following baseline values were obtained during wakefulness (perfusion rate, 0.3 microl/min): glucose, 1.7+/-0.9 mmol/L; lactate, 2.9+/-0.9 mmol/L; pyruvate, 166+/-47 micromol/L; lactate/pyruvate ratio, 23+/-4; glycerol, 82+/-44 micromol/L; glutamate, 16+/-16 mmol/L; urea, 4.4+/-1.7 mmol/L. Marked increases in the levels of all chemical markers were observed at the beginning and end of anesthesia/surgery. CONCLUSION: The study provides human baseline levels for biochemical markers that can presently be measured at the bedside during neurointensive care. In addition, some changes that occurred under varying physiological conditions are described.
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| 47. |
- Samuelson, Patrik, et al.
(författare)
-
Staphylococcal surface display of metal-binding polyhistidyl peptides
- 2000
-
Ingår i: Applied and Environmental Microbiology. - 0099-2240 .- 1098-5336. ; 66:3, s. 1243-1248
-
Tidskriftsartikel (refereegranskat)abstract
- Recombinant Staphylococcus xylosus and Staphylococcus carnosus strains were generated with surface-exposed chimeric proteins containing polyhistidyl peptides designed for binding to divalent metal ions. Surface accessibility of the chimeric surface proteins was demonstrated and the chimeric surface proteins were found to be functional in terms of metal binding, since the recombinant staphylococcal cells were shown to have gained Ni2+- and Cd2+-binding capacity, suggesting that such bacteria could find use in bioremediation of heavy metals. This is, to our knowledge, the first time that recombinant, surface-exposed metal-binding peptides have been expressed on gram-positive bacteria. Potential environmental or biosensor applications for such recombinant staphylococci as biosorbents are discussed.
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| 48. |
- Sandin, Gunnar, et al.
(författare)
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Aesthetic regulation of objectionable representations. : A semiotic approach to memorials and religious architectural space.
- 2012
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Ingår i: Global Semiotics : Bridging Different Civilizations - Bridging Different Civilizations. - 9787563037438
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Konferensbidrag (refereegranskat)abstract
- Whenever a material object is selected to represent a physically absent, but emotionally present referent, such as a religious figure or a deceased relative, we have a complex representation situation that involves what we could tentatively label “transgressive cognitive acts”. A transgressive cognitive act is here meant to involve a part that is experienced as present, and as having an effect, but not evidenced from a rationalist or scientific perspective on existence. Sometimes, the complexity of this type of representation is such that argumentation beside the theological, e.g. aesthetic, comes to stand as vicarious to theological or existential opinions about the matter. The semiotics of such vicarious representation have to consider a “leaking” communication model where one part in the trinity - of the sender, the receiver or the referent - escape a coherent or evidenced definition (God can be all three, depending on type of communication). The fact that empirically non-existing referents have been recognized and theorized in semiotics (Eco; Sonesson; and others) does not explain why certain cases of absent semiotic agents, linked to religious belief, or to sense of presence in grief of a deceased person, may give rise to such extreme degree of emotion or sense of existence.Departing from a narrated episode written by J M Coetzee, about the controversy brought up by a crucifix, turning into a quarrel about aesthetic preferences, this paper discusses a series of designed or spontaneously created spatial representations of physically absent, but indirectly and emotionally present referents, as they appear in memorials, cemeteries and funeral chapels. In public acts of memorialisation, the physical objects that represent the deceased – like gravestones or memorial objects placed at sites representing the dead – are often strictly regulated, differently in different cultures. When for instance they appear at unexpected places in the urban environment, or with unfamiliar looks, memorials and their material culture can become highly controversial or be regarded as objectionable.These materialisations with a relation to religion, or existential belief, may give rise to arguments where the aesthetic aspect is the last chance to state a principle order in the cultural scheme in which they are debated. Such arguments are actualised in the current debate about post-secular societies, where it is for instance stated (Habermas) that both religious and secular mentalities must be open to a complementary learning if we are to balance shared citizenship and cultural difference in the post-secular society. It is an objective of this paper to show that the recognition of a semiotics that involves the paradoxical presence of emotionally strong, but fundamentally absent parts in communication can contribute to the aesthetics of physically present, but existentially debated objects, as well as to the discussion about the confronting as well as conjoining elements of regulation of these issues in society.
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| 49. |
- Sandin, Gunnar, et al.
(författare)
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Aesthetic replacement strategies in hospitals
- 2011
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Ingår i: Ambience11 : Where art, technology and design meet - Where art, technology and design meet. - 9789197557689 ; , s. 69-77
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Konferensbidrag (refereegranskat)
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| 50. |
- Sandin, Gunnar, et al.
(författare)
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Feedback: an Architectural Interpretation of a Painting by Meret Opppenheim
- 2009
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Ingår i: La Semiotica. Intersección entre la Naturaleza y la Cultura / Semiotics Bridging Nature and Culture / La sémotique: carrefour de la nature et de la culture. : Proceedings of the 6th Congress of the IASS-AIS, Guadalajara, Mexico, 13-19 July, 1999. - Proceedings of the 6th Congress of the IASS-AIS, Guadalajara, Mexico, 13-19 July, 1999..
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Konferensbidrag (refereegranskat)
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