| 1. |
- Eberhard, Jakob, et al.
(författare)
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Emotional disorders in testicular cancer survivors in relation to hypogonadism, androgen receptor polymorphism and treatment modality.
- 2010
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Ingår i: Journal of Affective Disorders. - : Elsevier BV. - 1573-2517 .- 0165-0327. ; 122, s. 260-266
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Tidskriftsartikel (refereegranskat)abstract
- PURPOSE: It has been documented that testicular germ cell cancer (TGCC) patients may be at increased risk of developing emotional distress (EMD). Hence, the aim of the present study was to investigate whether EMD is related to the presence of hypogonadism, androgen receptor (AR) polymorphism and/or treatment intensity. PATIENTS AND METHODS: Three to five years after treatment, testosterone and luteinizing hormone (LH) levels were measured in 165 TGCC patients. These patients also completed a questionnaire concerning mental health. EMD was measured by the Hospital Anxiety and Depression Scale (HADS). The androgen receptor (AR) gene has two polymorphic regions in exon I; glutamine encoding CAG and glycine encoding GGN repeats. Association between emotional disorders and AR polymorphisms as well as type of treatment was assessed. RESULTS: Neither anxiety (OR 1.0; 95% CI 0.40-2.4) nor depression (OR 1.1; 95% CI 0.20-6.4) were overrepresented in biochemically hypogonadal TGCC patients and no association between AR polymorphisms and EMD was found. Patients treated with >/=5 cycles of cisplatinum based chemotherapy due to refractory or relapsed disease were more prone to experiencing symptoms of anxiety (p=0.006), but not depression (p=0.38). CONCLUSIONS: Biochemical hypogonadism and AR polymorphism do not seem to be risk factors for EMD in TGCC patients. Patients with refractory or relapsed disease receiving >/=5 cycles of cisplatinum based chemotherapy may, to a higher degree than patients receiving less intense therapy, suffer from anxiety.
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| 2. |
- Eberhard, Jakob, et al.
(författare)
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Sexual Function in Men Treated for Testicular Cancer.
- 2009
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Ingår i: Journal of Sexual Medicine. - : Oxford University Press (OUP). - 1743-6109 .- 1743-6095. ; 6, s. 1979-1989
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Tidskriftsartikel (refereegranskat)abstract
- ABSTRACT Introduction. Testicular germ cell cancer (TGCC) patients may be at risk of developing sexual dysfunction after treatment. Aim. The aim of this study was to assess the prevalence of sexual dysfunctions in TGCC patients 3 to 5 years after treatment, and relate findings to biochemical hypogonadism, treatment intensity, and the expected prevalence in the Swedish male population. Methods. A questionnaire study on 129 consecutive TGCC patients 3 to 5 years post-treatment was performed. Comparators were an age-matched nationally representative group of men (N = 916) included in a study on sexual life in Sweden. Main Outcome Measures. Sexual functions (including erectile dysfunctional distress), time since last intercourse, sexual satisfaction, and experience of sexological treatment seeking were assessed using the same questions used in the epidemiological study on sexual life in Sweden. The findings in TGCC patients were correlated to biochemical signs of hypogonadism and type of oncological treatment: Surveillance, adjuvant chemotherapy, adjuvant radiotherapy, or standard doses of chemotherapy. Results. A higher proportion of TGCC patients than comparators were likely to report low sexual desire (odds ratio [OR] 6.7 [95% confidence interval {CI} 2.1-21]) as well as erectile dysfunction (OR 3.8 [95% CI 1.4-10]). No significant differences were observed regarding erectile dysfunctional distress, change of desire over time, interest in sex, premature or delayed ejaculation, time since last intercourse, need for or receiving sexual advice, or sexual satisfaction. Hypogonadism did not predict erectile dysfunction (OR 1.1 [95% CI 0.26-4.5]) or low sexual desire (OR 1.2 [95% CI 0.11-14]). Treatment modality had no obvious impact on sexual function. Conclusion. Men treated for testicular cancer had higher risk of having low sexual desire and erectile dysfunction 3 to 5 years after completion of therapy than comparators. These sexual dysfunctions were not significantly associated with treatment intensity or hypogonadism. Eberhard J, Ståhl O, Cohn-Cedermark G, Cavallin-Ståhl E, Giwercman Y, Rylander L, Eberhard-Gran M, Kvist U, Fugl-Meyer KS, and Giwercman A. Sexual function in men treated for testicular cancer. J Sex Med **;**:**-**.
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| 3. |
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| 4. |
- Albertsson, Anna-Karin, et al.
(författare)
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PLUS Borås : Om professionsutveckling och dokumentation
- 2009
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Konferensbidrag (övrigt vetenskapligt/konstnärligt)abstract
- PLUS betyder Projekt för Läsutveckling, Utvärdering och Samverkan. Det handlar om lässtimulerande arbete bland barn från nyfödd upp till 16 år och omfattar alltså alla barn- och skolbibliotekarier i Borås. Folkbiblioteken i Borås består av Stadsbiblioteket och nio kommundelsbibliotek. I kommundelarna är skol- och folkbiblioteken samorganiserade eller i nära samverkan. Samtliga högstadieskolor och flera låg- och mellanstadieskolor har fackutbildade bibliotekarier. Alla folkbiblioteksenheter har barnbibliotekarietjänster. Stadsbiblioteket ansvarar för att barnbibliotekarier och skolbibliotekarier träffas regelbundet i sina respektive nätverk. Läsfrämjande arbete bedrivs på alla bibliotek, drygt trettio personer arbetar med det på varsitt håll. Det finns mycket kunskap och gedigen erfarenhet men tid för reflektion och bollande med idéer för inspiration och metodikutveckling är mycket begränsad. Idéer till projektets innehåll baseras i strävan att knyta alla bibliotekarier som arbetar med språk- och läsutvecklingen till en gemensam bas och därmed skapa förutsättningar för ett utvecklingsarbete i hela kommunen.
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| 5. |
- Garcia, Maxime, et al.
(författare)
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Sarek: A portable workflow for whole-genome sequencing analysis of germline and somatic variants
- 2020
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Ingår i: F1000 Research. - : F1000 Research Ltd. - 2046-1402. ; 9
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Tidskriftsartikel (refereegranskat)abstract
- Whole-genome sequencing (WGS) is a fundamental technology for research to advance precision medicine, but the limited availability of portable and user-friendly workflows for WGS analyses poses a major challenge for many research groups and hampers scientific progress. Here we present Sarek, an open-source workflow to detect germline variants and somatic mutations based on sequencing data from WGS, whole-exome sequencing (WES), or gene panels. Sarek features (i) easy installation, (ii) robust portability across different computer environments, (iii) comprehensive documentation, (iv) transparent and easy-to-read code, and (v) extensive quality metrics reporting. Sarek is implemented in the Nextflow workflow language and supports both Docker and Singularity containers as well as Conda environments, making it ideal for easy deployment on any POSIX-compatible computers and cloud compute environments. Sarek follows the GATK best-practice recommendations for read alignment and pre-processing, and includes a wide range of software for the identification and annotation of germline and somatic single-nucleotide variants, insertion and deletion variants, structural variants, tumour sample purity, and variations in ploidy and copy number. Sarek offers easy, efficient, and reproducible WGS analyses, and can readily be used both as a production workflow at sequencing facilities and as a powerful stand-alone tool for individual research groups. The Sarek source code, documentation and installation instructions are freely available at https://github.com/nf-core/sarek and at https://nf-co.re/sarek/.
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| 6. |
- Gräslund, Susanne, et al.
(författare)
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A novel affinity gene fusion system allowing protein A-based recovery of non-immunoglobulin gene products
- 2002
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Ingår i: Journal of Biotechnology. - 0168-1656 .- 1873-4863. ; 99:1, s. 41-50
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Tidskriftsartikel (refereegranskat)abstract
- An expression vector system has been developed, taking advantage of a novel, Staphylococcus aureus protein A (SPA)-binding affinity tag ZSPA-1, enabling straightforward affinity blotting procedures and efficient recovery by affinity purification of expressed gene products on readily available reagents and chromatography media. The 58 amino acid SPA-binding affinity tag ZSPA-1, was previously selected from a library constructed by combinatorial mutagenesis of a protein domain from SPA. An Escherichia coli expression vector for intracellular T7 promoter (PT7) driven production was constructed with an N-terminal dual affinity tag, consisting of a hexahistidyl (His6) tag in frame with the ZSPA-1 tag, thus allowing alternative affinity recovery methods. To evaluate the system, five cDNA clones from a mouse testis cDNA library were expressed, and two alternative blotting procedures were developed for convenient screening of expression efficiencies. The five produced fusion proteins were recovered on both immobilized metal-ion affinity chromatography (IMAC) columns and on Protein A-based chromatography media, to allow comparative studies. It was found that the Protein A-based recovery resulted in the highest degree of purity, and furthermore, gene products that were produced as inclusion bodies could after denaturation be efficiently affinity purified on Protein A-Sepharose in the presence of 0.5 M guanidine hydrochloride. The convenience and robustness of the presented expression system should make it highly suitable for various high-throughput protein expression efforts.
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| 7. |
- Hedenmalm, Karin, 1963-, et al.
(författare)
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Glucose intolerance with atypical antipsychotics
- 2002
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Ingår i: Drug Safety. - 0114-5916 .- 1179-1942. ; 25:15, s. 1107-1116
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Tidskriftsartikel (refereegranskat)abstract
- BACKGROUND: Previous studies have suggested that the atypical antipsychotics clozapine and olanzapine may be associated with an increased risk of glucose intolerance and diabetes mellitus. Early studies have also suggested an association between use of conventional antipsychotics and the development of glucose intolerance. OBJECTIVE: To examine quantitatively the association between glucose intolerance including diabetes mellitus and the use of the atypical antipsychotics clozapine, olanzapine or risperidone, and to identify possible risk factors for the development of glucose intolerance during treatment with these drugs. METHODS: All reports suggestive of glucose intolerance for clozapine, olanzapine and risperidone were identified in the WHO database for adverse drug reactions. In the analyses of possible risk factors for glucose intolerance all other reports of adverse drug reactions for clozapine, olanzapine and risperidone were used as reference. Using the Bayesian Confidence Propagation Neural Network method, the strengths of the associations over time between glucose intolerance and the use of these drugs were analysed. For comparison, the strengths of the associations between glucose intolerance and the use of the conventional antipsychotics haloperidol and chlorpromazine were also analysed. RESULTS: Clozapine, olanzapine and risperidone were significantly associated with glucose intolerance. In contrast, chlorpromazine and haloperidol were not associated with glucose intolerance. For clozapine, olanzapine and risperidone grouped together, the following potential risk factors for glucose intolerance were identified: an underlying diabetic condition (odds ratio [OR] 10.22, 95% CI 8.20-12.73), an increase in weight (OR 2.36, 95% CI 1.76-3.17), male gender (OR 1.27, 95% CI 1.11-1.47), and concomitant use of valproic acid (OR 1.97, 95% CI 1.61-2.40), selective serotonin reuptake inhibitors (OR 1.63, 95% CI 1.33-1.99) or buspirone (OR 2.24, 95% CI 1.33-3.77). CONCLUSION: Treatment with clozapine, olanzapine or risperidone appears to be associated with an increased risk of glucose intolerance.
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| 8. |
- Johansson, Daniel, et al.
(författare)
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Equivalent Chip Thickness and its Influence on Tool Life
- 2018
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Ingår i: Procedia Manufacturing. - : Elsevier BV. - 2351-9789. ; 25, s. 344-350
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Tidskriftsartikel (refereegranskat)abstract
- This paper investigates the accuracy of using the Woxén equivalent chip thickness to represent feed, depth of cut, nose radius and major cutting angle in tool life modeling of machining low alloy steel in longitudinal turning. Hägglund’s way of calculating the equivalent chip thickness has been used and compared to the Woxén equation. The equivalent chip thickness was held constant as the tool life was recorded for varied feeds and depths of cut. The results show that the tool life decreases for an increase of depth of cut and a decrease of feed, using the same equivalent chip thickness.
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| 9. |
- Johansson, Daniel, et al.
(författare)
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Small scale testing of PCD and WC-Co tooling in rock cutting using longitudinal turning
- 2019
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Ingår i: Wear. - : Elsevier BV. - 0043-1648. ; 426-427:Part B, s. 1515-1522
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Tidskriftsartikel (refereegranskat)abstract
- Rock cutting and crushing stands for a considerable part of the world use of Cemented Carbide (CC). CC is preferred due to its hardness while maintaining high fracture toughness. However, new cutting tool materials are developed allowing increased efficiency whilst minimizing the use of critical raw material such as tungsten and cobalt. One material showing promising capabilities is Polycrystalline Diamond (PCD). Field testing of new wear resistant tool materials are known to be notoriously difficult due to issues controlling the test environment. To further support the development of new and more wear resistant tool materials there is a need for an improved methodology of laboratory testing of rock cutting. In this work a test rig was developed and testing methodology was validated, when using cutting tool inserts with standard shapes, such as round double sided general purpose insert RNGN and dome shaped buttons while machining and crushing bars of red granite by longitudinal turning using a standard lathe. Optical microscopy, 3D data of Volume Difference Measurements and SEM were used for analyzing tool wear. Different grades of CC showed similar wear mechanisms irrespectively of tool geometry. The PCD grade did not show similar wear mechanisms, but did outperform CC grade in regard to wear resistance.
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| 10. |
- Linde, Anna-Malin, et al.
(författare)
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Complete genome characterisation of a Newcastle disease virus isolated during an outbreak in Sweden in 1997
- 2010
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Ingår i: Virus Genes. - : Springer Science and Business Media LLC. - 0920-8569 .- 1572-994X. ; 41, s. 165-173
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Tidskriftsartikel (refereegranskat)abstract
- The complete genome sequence of a Newcastle disease virus (NDV) isolated from a chicken in Sweden was determined and compared with other NDV sequences. The isolate was shown to belong to genotype VIIb, which arose in the Far East and spread around the world during the 1990s. It had a length of 15,192 bases and consisted of six genes in the order 3'-NP-P-M-F-HN-L-5'. The F protein cleavage site was 112-RRQRRF-117, corresponding to that of a virulent pathotype.
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| 11. |
- Munir, Muhammad, et al.
(författare)
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Complete Genome Analysis of an Avian Paramyxovirus Type 1 Strain Isolated in 1994 from an Asymptomatic Black-Headed Gull (Larus ridibundus) in Southern Sweden
- 2010
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Ingår i: Avian Diseases. - 0005-2086. ; 54, s. 923-930
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Tidskriftsartikel (refereegranskat)abstract
- The complete genome sequence of an avian paramyxovirus serotype 1 (APMV-1) isolated from a black-headed gull (Larus ridibundus) in Sweden was determined and compared with other APMV-1 sequences. Sequence analyses showed that this isolate consists of six genes in the order 3'-NP-P-M-F-HN-L-5', is 15,186 nucleotides long, and contains a typical, avirulent fusion protein cleavage site. It was also shown to have a hemagglutinin-neuraminidase protein with a length of 585 amino acids (aa) instead of the expected 616 aa. Phylogenetic analyses showed that the isolate belongs to genotype I, and the relationship with some other, known APMV-1 virus sequences was revealed. Waterfowl have been considered to act as a reservoir for APMV-1 and, therefore, it is important to broaden the knowledge of viruses circulating within this population.
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| 12. |
- Munir, Muhammad, et al.
(författare)
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Whole genome sequencing and characterization of a virulent Newcastle disease virus isolated from an outbreak in Sweden
- 2011
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Ingår i: Virus Genes. - : Springer Science and Business Media LLC. - 0920-8569 .- 1572-994X. ; 43, s. 261-271
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Tidskriftsartikel (refereegranskat)abstract
- In this study, the complete genome sequence of a Newcastle disease virus (NDV) isolate collected from an outbreak in 1995 in chickens was fully characterized and compared with other NDV sequences. The genome was found to be 15,192 nucleotides long and to consist of six genes in the order 3'-NP-P-M-F-HN-L-5', similar to other avian paramyxoviruses type-I. However, a six-nucleotide insertion was observed in the 5' non-coding regions of the nucleoprotein (NP) gene, a feature that is unique to some NDV isolates. The isolate shows the amino acid sequence (112)RRQKRF(117) at the cleavage site of the F protein, which is identical to a known motif for virulent pathotypes of NDV. The phylogenetic analysis of the coding region of the F gene indicated that this isolate belongs to genotype VI, more specifically to genotype VId, along with isolates from the other European countries (Denmark, Switzerland and Austria). The same genotype caused outbreaks in the Middle East and Greece in the late 1960s, and in Hungary, in the early 1980s, suggesting a common source for these outbreaks.
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| 13. |
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| 14. |
- Persson, Jonas, et al.
(författare)
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Discovery, optimization and biodistribution of an Affibody molecule for imaging of CD69
- 2021
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Ingår i: Scientific Reports. - : Springer Nature. - 2045-2322. ; 11:1
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Tidskriftsartikel (refereegranskat)abstract
- Due to the wide scale of inflammatory processes in different types of disease, more sensitive and specific biomarkers are required to improve prevention and treatment. Cluster of differentiation 69 (CD69) is one of the earliest cell surface proteins expressed by activated leukocytes. Here we characterize and optimize potential new imaging probes, Affibody molecules targeting CD69 for imaging of activated immune cells. Analysis of candidates isolated in a previously performed selection from a Z variant E. coli library to the recombinant extracellular domain of human CD69, identified one cross-reactive Z variant with affinity to murine and human CD69. Affinity maturation was performed by randomization of the primary Z variant, followed by selections from the library. The resulting Z variants were evaluated for affinity towards human and murine CD69 and thermal stability. The in vivo biodistribution was assessed by SPECT/CT in rats following conjugation of the Z variants by a DOTA chelator and radiolabeling with Indium-111. A primary Z variant with a K-d of approximately 50 nM affinity to human and murine CD69 was identified. Affinity maturation generated 5 additional Z variants with improved or similar affinity. All clones exhibited suitable stability. Radiolabeling and in vivo biodistribution in rat demonstrated rapid renal clearance for all variants, while the background uptake and washout varied. The variant Z(CD69:4) had the highest affinity for human and murine CD69 (34 nM) as well as the lowest in vivo background binding. In summary, we describe the discovery, optimization and evaluation of novel Affibody molecules with affinity for CD69. Affibody molecule Z(CD69:4) is suitable for further development for imaging of activated immune cells.
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