| 1. |
- Gaballa, A., et al.
(författare)
-
Assessment of TREC, KREC and telomere length in long-term survivors after allogeneic HSCT : the role of GvHD and graft source and evidence for telomere homeostasis in young recipients
- 2018
-
Ingår i: Bone Marrow Transplantation. - : Nature Publishing Group. - 0268-3369 .- 1476-5365. ; 53:1, s. 69-77
-
Tidskriftsartikel (refereegranskat)abstract
- Reconstitution of the adaptive immune system following allogeneic hematopoietic stem cell transplantation is crucial for beneficial outcome and is affected by several factors, such as GvHD and graft source. The impact of these factors on immune reconstitution has been thoroughly investigated during the early phase after transplantation. However, little is known about their long-term effect. Similarly, leukocyte telomere length (TL) shortening has been reported shortly after transplantation. Nevertheless, whether TL shortening continues in long-term aspect is still unsettled. Here, we assessed T-cell receptor excision circle (TREC), kappa deleting recombination excision circle (KREC) and leukocyte TL in recipients and donors several years post transplantation (median 17 years). Our analysis showed that, recipients who received bone marrow (BM) as the graft source have higher levels of both TREC and KREC. Also, chronic GvHD affected TREC levels and TL but not KREC levels. Finally, we show that recipient's TL was longer than respective donors in a group of young age recipients with high KREC levels. Our results suggest that BM can be beneficial for long-term adaptive immune recovery. We also present supporting evidence for recipient telomere homeostasis, especially in young age recipients, rather than telomere shortening.
|
|
| 2. |
|
|
| 3. |
- Gaballa, A, et al.
(författare)
-
Revisiting the Role of γδ T Cells in Anti-CMV Immune Response after Transplantation
- 2021
-
Ingår i: Viruses. - : MDPI AG. - 1999-4915. ; 13:6
-
Tidskriftsartikel (refereegranskat)abstract
- Gamma delta (γδ) T cells form an unconventional subset of T lymphocytes that express a T cell receptor (TCR) consisting of γ and δ chains. Unlike conventional αβ T cells, γδ T cells share the immune signature of both the innate and the adaptive immunity. These features allow γδ T cells to act in front-line defense against infections and tumors, rendering them an attractive target for immunotherapy. The role of γδ T cells in the immune response to cytomegalovirus (CMV) has been the focus of intense research for several years, particularly in the context of transplantation, as CMV reactivation remains a major cause of transplant-related morbidity and mortality. Therefore, a better understanding of the mechanisms that underlie CMV immune responses could enable the design of novel γδ T cell-based therapeutic approaches. In this regard, the advent of next-generation sequencing (NGS) and single-cell TCR sequencing have allowed in-depth characterization of CMV-induced TCR repertoire changes. In this review, we try to shed light on recent findings addressing the adaptive role of γδ T cells in CMV immunosurveillance and revisit CMV-induced TCR reshaping in the era of NGS. Finally, we will demonstrate the favorable and unfavorable effects of CMV reactive γδ T cells post-transplantation.
|
|
| 4. |
|
|
| 5. |
|
|
| 6. |
|
|
| 7. |
- Erkers, T, et al.
(författare)
-
Lymphocytes in Placental Tissues: Immune Regulation and Translational Possibilities for Immunotherapy
- 2017
-
Ingår i: Stem cells international. - : Hindawi Limited. - 1687-966X .- 1687-9678. ; 2017, s. 5738371-
-
Tidskriftsartikel (refereegranskat)abstract
- Immune modulation at the fetomaternal interface is crucial to ensure that the fetal allograft is not rejected. In the present review, the focus is to describe basic functions of lymphocyte populations and how they may contribute to fetomaternal immune regulation, as well as determining what proportions and effector functions of these cells are reported to be present in placental tissues in humans. Also explored is the possibility that unique cell populations at the fetomaternal interface may be targets for adoptive cell therapy. Increasing the understanding of immune modulation during pregnancy can give valuable insight into other established fields such as allogeneic hematopoietic stem cell transplantation and solid organ transplantation. In these settings, lymphocytes are key components that contribute to inflammation and rejection of either patient or donor tissues following transplantation. In contrast, an allogeneic fetus eludes rejection by the maternal immune system.
|
|
| 8. |
|
|
| 9. |
|
|
| 10. |
|
|
| 11. |
- Sairafi, D, et al.
(författare)
-
Donor Cell Composition and Reactivity Predict Risk of Acute Graft-versus-Host Disease after Allogeneic Hematopoietic Stem Cell Transplantation
- 2016
-
Ingår i: Journal of immunology research. - : Hindawi Limited. - 2314-7156 .- 2314-8861. ; 2016, s. 5601204-
-
Tidskriftsartikel (refereegranskat)abstract
- Background. Graft-versus-host disease (GVHD) is a serious complication after allogeneic hematopoietic stem cell transplantation (HSCT). We designed a functional assay for assessment of individual risk for acute GVHD.Study Design and Methods. Blood samples were collected from patients and donors before HSCT. Two groups of seven patients each were selected, one in which individuals developed acute GVHD grades II–IV and one in which none showed any clinical signs of GVHD. Peripheral blood mononuclear cells (PBMCs) isolated from donors were incubated in mixed lymphocyte cultures (MLCs) with recipient PBMCs. The cells were characterized by flow cytometry before and after MLC.Results. Samples from donors in the GVHD group contained significantly lower frequencies of naïveγδT-cells and T-cells expressing NK-cell markers CD56 and CD94. Donor samples in this group also exhibited lower frequencies of naïve CD95+T-cells compared to controls. After MLC, there were dissimilarities in the CD4/CD8 T-cell ratio and frequency of CD69+T-cells between the two patient groups, with the non-GVHD group showing higher frequencies of CD8+and CD69+T-cells.Conclusion. We conclude that a thorough flow cytometric analysis of donor cells for phenotype and allogeneic reactivity may be of value when assessing pretransplant risk for severe acute GVHD.
|
|
| 12. |
|
|
| 13. |
|
|
| 14. |
|
|
| 15. |
|
|
| 16. |
|
|
| 17. |
|
|
| 18. |
|
|
| 19. |
|
|
| 20. |
|
|
| 21. |
- Stikvoort, Britt, 1989-
(författare)
-
Why act sustainable? : Exploring what can be learnt from different approaches to motivations for pro-environmental behaviour
- 2022
-
Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- To combat anthropogenic climate change, greenhouse gas emissions need to be reduced. Though one person’s actions cannot turn the tide, so to say, the combined effort of many individuals can. To this end, numerous studies have investigated theoretically supported motivations – for example financial, environmental, hedonistic, moral, or normative motivations – underlying pro-environmental behaviours. To understand the causal relationship between these motivations and behaviours, studies tend to look at how such variables are associated across individuals. In observational studies, it is not easy to infer whether associations indicate a causal process or emerge from confounding pathways. This implies that positive associations between motivations and behaviour in an observational study do not necessarily imply that increasing individuals’ motivations will increase pro-environmental behaviour.This thesis presents and discusses studies that investigated associations between motivations and intentions to engage in pro-environmental behaviour using two different research designs – observational and experimental – and by looking at inter- and intraindividual variation in observational data. Papers I-III reveal that in data from observational designs, associations between motivations and intentions differ contingent on whether one uses an inter- or intraindividual approach to variation. Concretely, while financial and normative motivations were not predictive of variation in intentions between individuals, they were predictive of variation in intentions within individuals. That is, those with stronger financial and normative motivations compared to others did not have stronger intentions, per se, yet, when an individual reported a stronger financial or normative motivation for a specific behaviour compared to other behaviours, they tended to have stronger intentions towards the behaviour. In Paper III, an experimental manipulation that raised environmental motivations was not found to raise intentions. Overall, when associations are investigated in a way that is more closely aligned with the theoretically proposed mechanism (i.e., causal processes occurring within individuals), there seems more support for the motivational hypotheses predicted by theories.
|
|
| 22. |
|
|
| 23. |
|
|
