| 1. |
|
|
| 2. |
|
|
| 3. |
|
|
| 4. |
- Kindgren, Peter, et al.
(författare)
-
A novel proteomic approach reveals a role for Mg-protoporphyrin IX in response to oxidative stress
- 2011
-
Ingår i: Physiologia Plantarum. - : Wiley. - 0031-9317 .- 1399-3054. ; 141:4, s. 310-320
-
Tidskriftsartikel (refereegranskat)abstract
- The presence of genes encoding organellar proteins in different cellular compartments necessitates a tight coordination of expression by the different genomes of the eukaryotic cell. This coordination of gene expression is achieved by organelle-to-nucleus communication. Stress-induced perturbations of the tetrapyrrole pathway trigger large changes in nuclear gene expression. In order to investigate whether the tetrapyrrole Mg-ProtoIX itself is an important part of plastid-to-nucleus communication, we used an affinity column containing Mg-ProtoIX covalently linked to an Affi-Gel matrix. The proteins that bound to Mg-ProtoIX were analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis combined with nano liquid chromatography–mass spectrometry (MS)/MS. Thus, we present a novel proteomic approach to address the mechanisms involved in cellular signaling and we identified interactions between Mg-ProtoIX and a large number of proteins associated with oxidative stress responses. Our approach revealed an interaction between Mg-ProtoIX and the heat shock protein 90-type protein, HSP81-2 suggesting that a regulatory complex including HSP90 proteins and tetrapyrroles controlling gene expression is evolutionarily conserved between yeast and plants. In addition, our list of putative Mg-ProtoIX-binding proteins demonstrated that binding of tetrapyrroles does not depend on a specific amino acid motif but possibly on a specific fold of the protein.
|
|
| 5. |
- Murari, A., et al.
(författare)
-
A control oriented strategy of disruption prediction to avoid the configuration collapse of tokamak reactors
- 2024
-
Ingår i: Nature Communications. - 2041-1723 .- 2041-1723. ; 15:1
-
Tidskriftsartikel (refereegranskat)abstract
- The objective of thermonuclear fusion consists of producing electricity from the coalescence of light nuclei in high temperature plasmas. The most promising route to fusion envisages the confinement of such plasmas with magnetic fields, whose most studied configuration is the tokamak. Disruptions are catastrophic collapses affecting all tokamak devices and one of the main potential showstoppers on the route to a commercial reactor. In this work we report how, deploying innovative analysis methods on thousands of JET experiments covering the isotopic compositions from hydrogen to full tritium and including the major D-T campaign, the nature of the various forms of collapse is investigated in all phases of the discharges. An original approach to proximity detection has been developed, which allows determining both the probability of and the time interval remaining before an incoming disruption, with adaptive, from scratch, real time compatible techniques. The results indicate that physics based prediction and control tools can be developed, to deploy realistic strategies of disruption avoidance and prevention, meeting the requirements of the next generation of devices.
|
|
| 6. |
- Strand, Thomas, 1970-, et al.
(författare)
-
An intervention-based study of how MRI is perceived by patients with spinal metastasis after adjustments to the examination procedures
- 2018
-
Ingår i: Journal of Radiology Nursing. - Philadelphia, PA : Elsevier. - 1546-0843 .- 1555-9912. ; 37:2, s. 119-125
-
Tidskriftsartikel (refereegranskat)abstract
- The aim was to explore magnetic resonance imaging (MRI) experiences of patients with spinal metastasis after adjustments to the examination procedures have been made in accordance with the findings from a previous study. MRI is an important medical technology, which is considered to be the first choice of examination method when diagnosing and evaluating spinal metastatic tumors. It is a challenge to care for patients who experience anxiety and pain during an MRI. However, several aspects of the examination can be adjusted to improve the care for these patients. Findings from previous research were used to develop a care intervention, the effects of which are explored in this study. Qualitative deductive-inductive content analysis was used in this study. Eleven patients with spinal metastasis were interviewed about their experiences of going through an MRI scan based on an intervention designed in accordance with the findings from previous research. The findings showed that adjustments to the examination often were perceived as beneficial. However, patients needed to be involved in the decisions that influenced their own care. Time was an important component that affected the need for being prepared as well as the degree of personalization of the examination. This study shows that patients need to be seen as unique individuals, and they need to be able to influence the care that is given to them. The personalization of and adjustments to the examination routines need to be carried out in agreement with the patient.
|
|
| 7. |
- Strand, Thomas, 1970-
(författare)
-
Hjälp mig att hjälpa dig! : upplevelser och uppfattningar av undersökning med MRT för personer med metastaser i ryggen
- 2018
-
Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- Aim: The overall aim was to explore how patients with spinal metastasesexperience a magnetic resonance imaging examination (MRI). Furthermore,the purpose was to investigate the radiographers’ perceptions of the patients’care needs and what measures can be taken to relieve the suffering associatedwith an MRI examination.Methods: The design for all four studies was qualitative with content analysisused in Studies I, III and IV and a phenomenographic approach in Study II.Main Findings: The results showed that the patients could experience worry,anxiety, insecurity and pain during an MRI examination. These adverse feelingscan be reduced by adjustments to the examination’s routines as well as byadjustments to the examination’s settings. A short break in the middle of theexamination can be beneficial if the patient is involved in the decision regardingthe break or other adjustments made in conjunction with the examination.Radiographers’ perceptions of the caring for patients were influenced by theircaring perspective and their approach towards what they consider to be essentialin the care of patients with spinal metastasis. The radiographers used theirintuition as a moral compass when they decided what, when and how to adjustthe different aspects of the MRI examination. A shortage of time can affect theextent of the adjustments that can be made as well as the establishment of acaring relationship with the patient.Conclusions: The patients may feel a sense of security when they receive correctinformation prior to and during the examination. Patients want to influencetheir own care. The personalization and adjustments of the examinationroutines need to be performed in agreement with the patient. The radiographersshould not use intuition as the foundation for their care and assumptions aboutthe care needs of the patients should be avoided. The
|
|
| 8. |
- Strand, Thomas, 1970-, et al.
(författare)
-
The Experience of Patients With Neoplasm Metastasis in the Spine During a Magnetic Resonance Imaging Examination
- 2014
-
Ingår i: Journal of Radiology Nursing. - Philadelphia, PA : Elsevier. - 1546-0843 .- 1555-9912. ; 33:4, s. 191-198
-
Tidskriftsartikel (refereegranskat)abstract
- The aim of the study was to explore the experience of a magnetic resonance imaging (MRI) examination by patients with neoplasm metastasis in the spine. MRI is the most accurate method to diagnose and evaluate suspected metastatic disease in the spine. Patients may experience anxiety because of the fear of pain, fear of the unknown, and the apprehension about what the test might reveal. The study had a qualitative design, and the collected data were analyzed by means of latent content analysis. Twelve semistructured in-depth interviews were carried out starting with the question “Can you tell me about your experience of the MRI examination?” Four themes were identified: “motivation,” “worry and anxiety,” “insecurity,” and “security.” The patients were highly motivated to be examined by MRI, although most of them did experience some degree of worry or anxiety. The level of worry or anxiety was generated by the perception that an MRI examination was unpleasant, uncomfortable, or by the fear of what the result would show. All participants experienced some degree of insecurity, but in different ways, the insecurity of the patient could be reduced and the patients could experience a greater degree of security. The feelings of insecurity or security could be influenced by the radiographer, patients themselves, and MRI equipment. This study shows that most patients usually experience worry and anxiety. If the patients are motivated, they can manage to go through the examination in spite of the previously mentioned adverse feelings. Patients' feelings tend to fluctuate between a sense of insecurity and one of security. © 2014 Association for Radiologic & Imaging Nursing.
|
|
