| 1. |
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| 2. |
- Klionsky, Daniel J., et al.
(författare)
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Guidelines for the use and interpretation of assays for monitoring autophagy
- 2012
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Ingår i: Autophagy. - : Informa UK Limited. - 1554-8635 .- 1554-8627. ; 8:4, s. 445-544
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Forskningsöversikt (refereegranskat)abstract
- In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process vs. those that measure flux through the autophagy pathway (i.e., the complete process); thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from stimuli that result in increased autophagic activity, defined as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (in most higher eukaryotes and some protists such as Dictyostelium) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the field understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field.
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| 3. |
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- 2019
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Tidskriftsartikel (refereegranskat)
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| 4. |
- Bhat, Goutam, et al.
(författare)
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NTIRE 2022 Burst Super-Resolution Challenge
- 2022
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Ingår i: 2022 IEEE/CVF CONFERENCE ON COMPUTER VISION AND PATTERN RECOGNITION WORKSHOPS (CVPRW 2022). - : IEEE. - 9781665487399 - 9781665487405 ; , s. 1040-1060
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Konferensbidrag (refereegranskat)abstract
- Burst super-resolution has received increased attention in recent years due to its applications in mobile photography. By merging information from multiple shifted images of a scene, burst super-resolution aims to recover details which otherwise cannot be obtained using a simple input image. This paper reviews the NTIRE 2022 challenge on burst super-resolution. In the challenge, the participants were tasked with generating a clean RGB image with 4x higher resolution, given a RAW noisy burst as input. That is, the methods need to perform joint denoising, demosaicking, and super-resolution. The challenge consisted of 2 tracks. Track 1 employed synthetic data, where pixel-accurate high-resolution ground truths are available. Track 2 on the other hand used real-world bursts captured from a handheld camera, along with approximately aligned reference images captured using a DSLR. 14 teams participated in the final testing phase. The top performing methods establish a new state-of-the-art on the burst super-resolution task.
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| 5. |
- Deng, Min, et al.
(författare)
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Genome-wide association analyses in Han Chinese identify two new susceptibility loci for amyotrophic lateral sclerosis
- 2013
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Ingår i: Nature Genetics. - : Nature Publishing Group. - 1061-4036 .- 1546-1718. ; 45:6, s. 697-
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Tidskriftsartikel (refereegranskat)abstract
- To identify susceptibility genes for amyotrophic lateral sclerosis (ALS), we conducted a genome-wide association study (GWAS) in 506 individuals with sporadic ALS and 1,859 controls of Han Chinese ancestry. Ninety top SNPs suggested by the current GWAS and 6 SNPs identified by previous GWAS were analyzed in an independent cohort of 706 individuals with ALS and 1,777 controls of Han Chinese ancestry. We discovered two new susceptibility loci for ALS at 1q32 (CAMK1G, rs6703183, P-combined = 2.92 x 10(-8), odds ratio (OR) = 1.31) and 22p11 (CABIN1 and SUSD2, rs8141797, P-combined = 2.35 x 10(-9), OR = 1.52). These two loci explain 12.48% of the overall variance in disease risk in the Han Chinese population. We found no association evidence for the previously reported loci in the Han Chinese population, suggesting genetic heterogeneity of disease susceptibility for ALS between ancestry groups. Our study identifies two new susceptibility loci and suggests new pathogenic mechanisms of ALS.
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| 6. |
- Sun, Gang, et al.
(författare)
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Energy-Efficient Provisioning for Service Function Chains to Support Delay-Sensitive Applications in Network Function Virtualization
- 2020
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Ingår i: IEEE Internet of Things Journal. - : IEEE. - 2327-4662. ; 7:7, s. 6116-6131
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Tidskriftsartikel (refereegranskat)abstract
- The efficient deployment of virtual network functions (VNFs) for network service provisioning is key for achieving network function virtualization (NFV); however, most existing studies address only offline or one-off deployments of service function chains (SFCs) while neglecting the dynamic (i.e., online) deployment and expansion requirements. In particular, many methods of energy/resource cost reduction are achieved by merging VNFs. However, the energy waste and device wear for large-scale collections of servers (e.g., cloud networks and data centers) caused by sporadic request updating are ignored. To solve these problems, we propose an energy-aware routing and adaptive delayed shutdown (EAR-ADS) algorithm for dynamic SFC deployment, which includes the following features. 1) Energy-aware routing (EAR): By considering a practical deployment environment, a flexible solution is developed based on reusing open servers and selecting paths with the aims of balancing energy and resources and minimizing the total cost. 2) Adaptive delayed shutdown (ADS): The delayed shutdown time of the servers can be flexibly adjusted in accordance with the usage of each device in each time slot, thus eliminating the no-load wait time of the servers and frequent on/off switching. Therefore, EAR-ADS can achieve dual energy savings by both decreasing the number of open servers and reducing the idle/switching energy consumption of these servers. Simulation results show that EAR-ADS not only minimizes the cost of energy and resources but also achieves an excellent success rate and stability. Moreover, EAR-ADS is efficient compared with an improved Markov algorithm (SAMA), reducing the average deployment time by more than a factor of 40.
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| 7. |
- Wang, Chengdong, et al.
(författare)
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The proto-oncogene transcription factor Ets1 regulates neural crest development through Histone Deacetylase 1 to mediate output of bone morphogenetic protein signaling.
- 2015
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Ingår i: Journal of Biological Chemistry. - 1083-351X. ; 290:36, s. 21925-21938
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Tidskriftsartikel (refereegranskat)abstract
- The neural crest (NC) is a transient, migratory cell population that differentiates into a large variety of tissues including craniofacial cartilage, melanocytes, and peripheral nervous system. NC is initially induced at the border of neural plate and non-neuralectoderm by balanced regulation of multiple signaling pathways, among which an intermediate bone morphogenetic protein (BMP) signaling is essential for NC formation. Ets1, a proto-oncogene playing important roles in tumor invasion, has also been implicated in delamination of NC cells. In this study, we investigated Ets1 function in NC formation using Xenopus. Overexpression of ets1 repressed NC formation through down-regulation of BMP signaling. Moreover, ets1 repressed the BMP-responsive gene id3 that is essential for NC formation. Conversely, overexpression of id3 can partially rescue the phenotype of NC inhibition induced by ectopic ets1. Mechanistically, we found that Ets1 binds to id3 promoter as well as Histone Deacetylase 1 (HDAC1), suggesting that Ets1 recruits HDAC1 to the promoter of id3, thereby inducing Histone deacetylation of the id3 promoter. Thus, our studies indicate that Ets1 regulates NC formation through attenuating BMP signaling epigenetically.
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| 8. |
- Wang, Fang, et al.
(författare)
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Emerging contaminants: A One Health perspective
- 2024
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Ingår i: Innovation. - 2666-6758. ; 5
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Forskningsöversikt (refereegranskat)abstract
- Environmental pollution is escalating due to rapid global development that often prioritizes human needs over planetary health. Despite global efforts to mitigate legacy pollutants, the continuous introduction of new substances remains a major threat to both people and the planet. In response, global initiatives are focusing on risk assessment and regulation of emerging contaminants, as demonstrated by the ongoing efforts to establish the UN's Intergovernmental Science-Policy Panel on Chemicals, Waste, and Pollution Prevention. This review identifies the sources and impacts of emerging contaminants on planetary health, emphasizing the importance of adopting a One Health approach. Strategies for monitoring and addressing these pollutants are discussed, underscoring the need for robust and socially equitable environmental policies at both regional and international levels. Urgent actions are needed to transition toward sustainable pollution management practices to safeguard our planet for future generations.
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| 9. |
- Xu, Kai, et al.
(författare)
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Ground-state electron transfer in all-polymer donor-acceptor heterojunctions
- 2020
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Ingår i: Nature Materials. - : Springer Science and Business Media LLC. - 1476-4660 .- 1476-1122. ; 19:7, s. 738-744
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Tidskriftsartikel (refereegranskat)abstract
- Doping of organic semiconductors is crucial for the operation of organic (opto)electronic and electrochemical devices. Typically, this is achieved by adding heterogeneous dopant molecules to the polymer bulk, often resulting in poor stability and performance due to dopant sublimation or aggregation. In small-molecule donor–acceptor systems, charge transfer can yield high and stable electrical conductivities, an approach not yet explored in all-conjugated polymer systems. Here, we report ground-state electron transfer in all-polymer donor–acceptor heterojunctions. Combining low-ionization-energy polymers with high-electron-affinity counterparts yields conducting interfaces with resistivity values five to six orders of magnitude lower than the separate single-layer polymers. The large decrease in resistivity originates from two parallel quasi-two-dimensional electron and hole distributions reaching a concentration of ∼1013 cm–2. Furthermore, we transfer the concept to three-dimensional bulk heterojunctions, displaying exceptional thermal stability due to the absence of molecular dopants. Our findings hold promise for electro-active composites of potential use in, for example, thermoelectrics and wearable electronics.
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| 10. |
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| 11. |
- Chen, Zhibin, et al.
(författare)
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Summary of the 3rd International Workshop on Gas-Dynamic Trap based Fusion Neutron Source (GDT-FNS)
- 2022
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Ingår i: Nuclear Fusion. - : IOP Publishing. - 0029-5515 .- 1741-4326. ; 62:6
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Tidskriftsartikel (refereegranskat)abstract
- The 3rd International Workshop on Gas-Dynamic Trap-based Fusion Neutron Source (GDT-FNS) was held through the hybrid mode on 13-14 September 2021 in Hefei, China, jointly organized by the Hefei Institutes of Physical Science (HFIPS), Chinese Academy of Sciences (CAS), and the Budker Institute of Nuclear Physics (BINP), Russian Academy of Sciences (RAS). It followed the 1st GDT-FNS Workshop held in November 2018 in Hefei, China, and the 2nd taking place in November 2019 in Novosibirsk, Russian Federation. With the financial support from CAS and China Association for Science and Technology (CAST), this workshop was attended by more than 80 participants representing 20 institutes and universities from seven countries, with oral presentations broadcast via the Zoom conferencing system. Twenty-two presentations were made with topics covering design and key technologies, simulation and experiments, steady-state operation, status of the ALIANCE project, multi applications of neutron sources, and other concepts (Tokamaks, Mirrors, FRC, Plasma Focus, etc). The workshop consensus was made including the establishment of the ALIANCE International Working Group. The next GDT-FNS workshop is planned to be held in May 2022 in Novosibirsk.
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| 12. |
- Christian, Parul, et al.
(författare)
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Risk of childhood undernutrition related to small-for-gestational age and preterm birth in low- and middle-income countries
- 2013
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Ingår i: International Journal of Epidemiology. - : Oxford University Press (OUP). - 0300-5771 .- 1464-3685. ; 42:5, s. 1340-1355
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Tidskriftsartikel (refereegranskat)abstract
- BACKGROUND:Low- and middle-income countries continue to experience a large burden of stunting; 148 million children were estimated to be stunted, around 30-40% of all children in 2011. In many of these countries, foetal growth restriction (FGR) is common, as is subsequent growth faltering in the first 2 years. Although there is agreement that stunting involves both prenatal and postnatal growth failure, the extent to which FGR contributes to stunting and other indicators of nutritional status is uncertain.METHODS:Using extant longitudinal birth cohorts (n = 19) with data on birthweight, gestational age and child anthropometry (12-60 months), we estimated study-specific and pooled risk estimates of stunting, wasting and underweight by small-for-gestational age (SGA) and preterm birth.RESULTS:We grouped children according to four combinations of SGA and gestational age: adequate size-for-gestational age (AGA) and preterm; SGA and term; SGA and preterm; and AGA and term (the reference group). Relative to AGA and term, the OR (95% confidence interval) for stunting associated with AGA and preterm, SGA and term, and SGA and preterm was 1.93 (1.71, 2.18), 2.43 (2.22, 2.66) and 4.51 (3.42, 5.93), respectively. A similar magnitude of risk was also observed for wasting and underweight. Low birthweight was associated with 2.5-3.5-fold higher odds of wasting, stunting and underweight. The population attributable risk for overall SGA for outcomes of childhood stunting and wasting was 20% and 30%, respectively.CONCLUSIONS:This analysis estimates that childhood undernutrition may have its origins in the foetal period, suggesting a need to intervene early, ideally during pregnancy, with interventions known to reduce FGR and preterm birth.
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| 13. |
- Dong, Gang, et al.
(författare)
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Mobility analysis and kinematic synthesis of a novel 4-DoF parallel manipulator
- 2016
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Ingår i: Robotica (Cambridge. Print). - 0263-5747 .- 1469-8668.
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Tidskriftsartikel (refereegranskat)abstract
- This paper proposes a novel parallel manipulator with 1 translational and 3 rotational degrees of freedom, which may be designed as the docking equipment for large-scale component assemblage in the aircraft industry. First, the mobility and kinematic analysis of the novel manipulator is performed using the screw theory and the closed-loop vector method. To evaluate the kinematic performance of the manipulator, its workspace is calculated, and the dimensional homogeneous Jacobian matrix of this manipulator is deduced. Mainly based on a nonlinear programming approach, the kinematic dimensional synthesis is performed to optimise the dimensional parameters of this novel parallel manipulator in a prescribed workspace. The results of this paper may lay a solid foundation for the prototype design and manufacture of the novel parallel manipulator.
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| 14. |
- Fan, Chuan Gang, et al.
(författare)
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Experimental study of sidewall effect on flame characteristics of heptane pool fires with different aspect ratios and orientations in a channel
- 2017
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Ingår i: Proceedings of the Combustion Institute. - : Elsevier BV. - 1540-7489 .- 1873-2704. ; 36:2, s. 3121-3129
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Tidskriftsartikel (refereegranskat)abstract
- A series of small scale tests was conducted to investigate the influence of sidewall on flame characteristics of heptane pool fires in a channel, considering pool shape (aspect ratio: 1, 2, 4 and 8) and pool orientation relative to sidewall. Distance between fire and sidewall was changed systematically. Both transverse flame development (along the direction of channel width) and longitudinal flame development (along the direction of channel length) were recorded by digital video. Results show that for a fixed fire location, the heat release rate increases with the increasing pool aspect ratio (namely a larger pool perimeter), which indicates more air entrainment and more intense combustion. In wall fire cases, when the long pool rim is perpendicular with channel sidewall, the flame can obtain more air entrainment with a weaker boundary restriction from the sidewall, compared to the case with the long pool rim being parallel with sidewall. Comparison of some previously established correlations based on various experimental conditions with our test results is made. Due to the fact without considering sidewall effect and fuel shape on the air entrainment of fire plume, the classic correlations need to be further improved. Therefore, an integral flame length model considering both sidewall effect and fuel shape is developed, which correlates well with all the data from cases with various pool positions, orientations and aspect ratios. © 2016 The Combustion Institute. Published by Elsevier Inc.
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| 15. |
- Fang, Du, et al.
(författare)
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Increased neuronal PreP activity reduces A beta accumulation, attenuates neuroinflammation and improves mitochondrial and synaptic function in Alzheimer disease's mouse model
- 2015
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Ingår i: Human Molecular Genetics. - : Oxford University Press (OUP). - 0964-6906 .- 1460-2083. ; 24:18, s. 5198-5210
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Tidskriftsartikel (refereegranskat)abstract
- Accumulation of amyloid-beta (A beta) in synaptic mitochondria is associated with mitochondrial and synaptic injury. The underlying mechanisms and strategies to eliminate A beta and rescue mitochondrial and synaptic defects remain elusive. Presequence protease (PreP), a mitochondrial peptidasome, is a novel mitochondrial A beta degrading enzyme. Here, we demonstrate for the first time that increased expression of active human PreP in cortical neurons attenuates Alzheimer disease's (AD)-like mitochondrial amyloid pathology and synaptic mitochondrial dysfunction, and suppresses mitochondrial oxidative stress. Notably, PreP-overexpressed AD mice show significant reduction in the production of proinflammatory mediators. Accordingly, increased neuronal PreP expression improves learning and memory and synaptic function in vivo AD mice, and alleviates A beta-mediated reduction of long-term potentiation (LTP). Our results provide in vivo evidence that PreP may play an important role in maintaining mitochondrial integrity and function by clearance and degradation of mitochondrial A beta along with the improvement in synaptic and behavioral function in AD mouse model. Thus, enhancing PreP activity/expression may be a new therapeutic avenue for treatment of AD.
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| 16. |
- Han, Xin-Bao, et al.
(författare)
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Ultrasmall Abundant Metal-Based Clusters as Oxygen-Evolving Catalysts
- 2019
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Ingår i: Journal of the American Chemical Society. - : American Chemical Society (ACS). - 0002-7863 .- 1520-5126. ; 141:1, s. 232-239
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Tidskriftsartikel (refereegranskat)abstract
- The oxygen evolution reaction is a crucial step in water electrolysis to develop clean and renewable energy. Although noble metal-based catalysts have demonstrated high activity for the oxygen evolution reaction, their application is limited by their high cost and low availability. Here we report the use of a molecule-to-cluster strategy for preparing ultrasmall trimetallic clusters by using the polyoxometalate molecule as a precursor. Ultrafine (0.8 nm) transition-metal clusters with controllable chemical composition are obtained. The transition-metal clusters enable highly efficient oxygen evolution through water electrolysis in alkaline media, manifested by an overpotential of 192 mV at 10 mA cm–2, a low Tafel slope of 36 mV dec–1, and long-term stability for 30 h of electrolysis. We note, however, that besides the excellent performance as an oxygen evolution catalyst, our molecule-to-cluster strategy provides a means to achieve well-defined transition-metal clusters in the subnanometer regime, which potentially can have an impact on several other applications.
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| 17. |
- Haycock, Philip C., et al.
(författare)
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Association Between Telomere Length and Risk of Cancer and Non-Neoplastic Diseases A Mendelian Randomization Study
- 2017
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Ingår i: JAMA Oncology. - : American Medical Association. - 2374-2437 .- 2374-2445. ; 3:5, s. 636-651
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Tidskriftsartikel (refereegranskat)abstract
- IMPORTANCE: The causal direction and magnitude of the association between telomere length and incidence of cancer and non-neoplastic diseases is uncertain owing to the susceptibility of observational studies to confounding and reverse causation. OBJECTIVE: To conduct a Mendelian randomization study, using germline genetic variants as instrumental variables, to appraise the causal relevance of telomere length for risk of cancer and non-neoplastic diseases. DATA SOURCES: Genomewide association studies (GWAS) published up to January 15, 2015. STUDY SELECTION: GWAS of noncommunicable diseases that assayed germline genetic variation and did not select cohort or control participants on the basis of preexisting diseases. Of 163 GWAS of noncommunicable diseases identified, summary data from 103 were available. DATA EXTRACTION AND SYNTHESIS: Summary association statistics for single nucleotide polymorphisms (SNPs) that are strongly associated with telomere length in the general population. MAIN OUTCOMES AND MEASURES: Odds ratios (ORs) and 95% confidence intervals (CIs) for disease per standard deviation (SD) higher telomere length due to germline genetic variation. RESULTS: Summary data were available for 35 cancers and 48 non-neoplastic diseases, corresponding to 420 081 cases (median cases, 2526 per disease) and 1 093 105 controls (median, 6789 per disease). Increased telomere length due to germline genetic variation was generally associated with increased risk for site-specific cancers. The strongest associations (ORs [ 95% CIs] per 1-SD change in genetically increased telomere length) were observed for glioma, 5.27 (3.15-8.81); serous low-malignant-potential ovarian cancer, 4.35 (2.39-7.94); lung adenocarcinoma, 3.19 (2.40-4.22); neuroblastoma, 2.98 (1.92-4.62); bladder cancer, 2.19 (1.32-3.66); melanoma, 1.87 (1.55-2.26); testicular cancer, 1.76 (1.02-3.04); kidney cancer, 1.55 (1.08-2.23); and endometrial cancer, 1.31 (1.07-1.61). Associations were stronger for rarer cancers and at tissue sites with lower rates of stem cell division. There was generally little evidence of association between genetically increased telomere length and risk of psychiatric, autoimmune, inflammatory, diabetic, and other non-neoplastic diseases, except for coronary heart disease (OR, 0.78 [ 95% CI, 0.67-0.90]), abdominal aortic aneurysm (OR, 0.63 [ 95% CI, 0.49-0.81]), celiac disease (OR, 0.42 [ 95% CI, 0.28-0.61]) and interstitial lung disease (OR, 0.09 [ 95% CI, 0.05-0.15]). CONCLUSIONS AND RELEVANCE: It is likely that longer telomeres increase risk for several cancers but reduce risk for some non-neoplastic diseases, including cardiovascular diseases.
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| 18. |
- Hu, Wenjun, et al.
(författare)
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Heterogeneous nuclear ribonucleoprotein L facilitates recruitment of 53BP1 and BRCA1 at the DNA break sites induced by oxaliplatin in colorectal cancer
- 2019
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Ingår i: Cell Death and Disease. - : NATURE PUBLISHING GROUP. - 2041-4889. ; 10
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Tidskriftsartikel (refereegranskat)abstract
- Although oxaliplatin is an effective chemotherapeutic drug for treatment of colorectal cancer (CRC), tumor cells can develop mechanisms to evade oxaliplatin-induced cell death and show high tolerance and acquired resistance to this drug. Heterogeneous nuclear ribonucleoprotein L (hnRNP L) has been proved to play a critical role in DNA repair during IgH class switch recombination (CSR) in B lymphocytes, while, its role in CRC and chemotherapeutic resistance remain unknown. Our study aims to uncover an unidentified mechanism of regulating DNA double-strand breaks (DSBs) by hnRNP L in CRC cells treated by oxaliplatin. In present study, we observed that knockdown of hnRNP L enhanced the level of DNA breakage and sensitivity of CRC cells to oxaliplatin. The expression of key DNA repair factors (BRCA1, 53BP1, and ATM) was unaffected by hnRNP L knockdown, thereby excluding the likelihood of hnRNP L mediation via mRNA regulation. Moreover, we observed that phosphorylation level of ATM changed oppositely to 53BP1 and BRCA1 in the CRC cells (SW620 and HCT116) which exhibit synergistic effect by oxaliplatin plus hnRNP L impairment. And similar phenomenon was observed in the foci formation of these critical repair factors. We also found that hnRNP L binds directly with these DNA repair factors through its RNA-recognition motifs (RRMs). Analysis of cell death indicated that the RRMs of hnRNP L are required for cell survival under incubation with oxaliplatin. In conclusion, hnRNP L is critical for the recruitment of the DNA repair factors in oxaliplatin-induced DSBs. Targeting hnRNP L is a promising new clinical approach that could enhance the effectiveness of current chemotherapeutic treatment in patients with resistance to oxaliplatin.
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| 19. |
- Hu, Yang, et al.
(författare)
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Low-Carbohydrate Diet Scores and Mortality Among Adults With Incident Type 2 Diabetes
- 2023
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Ingår i: Diabetes care. - : American Diabetes Association. - 1935-5548 .- 0149-5992. ; 46:4, s. 874-884
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Tidskriftsartikel (refereegranskat)abstract
- OBJECTIVE: The current study aims to prospectively examine the association between postdiagnosis low-carbohydrate diet (LCD) patterns and mortality among individuals with type 2 diabetes (T2D). RESEARCH DESIGN AND METHODS: Among participants with incident diabetes identified in the Nurses' Health Study and Health Professionals Follow-up Study, an overall total LCD score (TLCDS) was calculated based on the percentage of energy as total carbohydrates. In addition, vegetable (VLCDS), animal (ALCDS), healthy (HLCDS), and unhealthy (ULCDS) LCDS were further derived that emphasized different sources and quality of macronutrients. Multivariable-adjusted Cox models were used to assess the association between the LCDS and mortality. RESULTS: Among 10,101 incident T2D cases contributing 139,407 person-years during follow-up, we documented 4,595 deaths of which 1,389 cases were attributed to cardiovascular disease (CVD) and 881 to cancer. The pooled multivariable-adjusted hazard ratios (HRs, 95% CIs) of total mortality per 10-point increment of postdiagnosis LCDS were 0.87 (0.82, 0.92) for TLCDS, 0.76 (0.71, 0.82) for VLCDS, and 0.78 (0.73, 0.84) for HLCDS. Both VLCDS and HLCDS were also associated with significantly lower CVD and cancer mortality. Each 10-point increase of TLCDS, VLCDS, and HLCDS from prediagnosis to postdiagnosis period was associated with 12% (7%, 17%), 25% (19%, 30%), and 25% (19%, 30%) lower total mortality, respectively. No significant associations were observed for ALCDS and ULCDS. CONCLUSIONS: Among people with T2D, greater adherence to LCD patterns that emphasize high-quality sources of macronutrients was significantly associated with lower total, cardiovascular, and cancer mortality.
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| 20. |
- Ji, Jie, et al.
(författare)
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Experimental study of non-monotonous sidewall effect on flame characteristics and burning rate of n-heptane pool fires
- 2015
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Ingår i: Fuel. - : Elsevier BV. - 0016-2361 .- 1873-7153. ; 145, s. 228-233
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Tidskriftsartikel (refereegranskat)abstract
- To study the influence of sidewall effect on flame characteristics and burning rate, a series of experiments with heptane pools was conducted. The results showed that as the fires were placed close to the sidewall, the flames inclined to the sidewall due to the restriction on air entrainment, and the burning rate increased on the whole, which could be mainly due to the enhanced radiation from the heated sidewall and ceiling flame. However, regardless of fuel pool shape, the burning rate obtained the peak value when the fire was near the sidewall, rather than attached to the sidewall, resulting from less flame radiation from the vertical flame part to the fuel in the latter case. The ratio of longitudinal ceiling flame length to transverse length tended to decrease with the fire moving close to the sidewall. For cases with the largest length and wall fires, the ratio was nearly 0.5, which could be explained according to the theory of mirror effect. Also, due to the non-monotonous sidewall effect, a higher burning rate did not necessarily lead to a larger ceiling flame length.
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| 21. |
- Jiang, Ping-Li, et al.
(författare)
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Controllable degradation of medical magnesium by electrodeposited composite films of mussel adhesive protein (Mefp-1) and chitosan
- 2016
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Ingår i: Journal of Colloid and Interface Science. - : Academic Press. - 0021-9797 .- 1095-7103. ; 478, s. 246-255
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Tidskriftsartikel (refereegranskat)abstract
- To control the degradation rate of medical magnesium in body fluid environment, biocompatible films composed of Mussel Adhesive Protein (Mefp-1) and chitosan were electrodeposited on magnesium surface in cathodic constant current mode. The compositions and structures of the films were characterized by atomic force microscope (AFM), scanning electron microscope (SEM) and infrared reflection absorption spectroscopy (IRAS). And the corrosion protection performance was investigated using electrochemical measurements and immersion tests in simulated body fluid (Hanks' solution). The results revealed that Mefp-1 and chitosan successfully adhered on the magnesium surface and formed a protective film. Compared with either single Mefp-1 or single chitosan film, the composite film of chitosan/Mefp-1/chitosan (CPC (chitosan/Mefp-1/chitosan)) exhibited lower corrosion current density, higher polarization resistance and more homogenous corrosion morphology and thus was able to effectively control the degradation rate of magnesium in simulated body environment. In addition, the active attachment and spreading of MC3T3-E1 cells on the CPC film coated magnesium indicated that the CPC film was significantly able to improve the biocompatibility of the medical magnesium.
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| 22. |
- Jiang, Shu-Heng, et al.
(författare)
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Modeling of cancer-related body-wide effects identifies LTB4 as a diagnostic biomarker for pancreatic cancer
- 2022
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Ingår i: EBioMedicine. - : Elsevier BV. - 2352-3964. ; 80, s. 104050-104050
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Tidskriftsartikel (refereegranskat)abstract
- BACKGROUND: Cancer elicits a complex adaptive response in an organism. Limited information is available for the body-wide effects induced by cancer. Here, we evaluated multiorgan changes in mouse models of pancreatic ductal adenocarcinoma (PDAC) and its precursor lesions (pancreatic intraepithelial neoplasia, PanIN) to decipher changes that occur during PDAC development.METHODS: RNA-sequencing was employed in the brain, colon, stomach, kidney, heart, liver, and lung tissues of mice with PanIN and PDAC. A combination of differential expression analysis and functional-category enrichment was applied for an in-depth understanding of the multiorgan transcriptome. Differentially expressed genes were verified by quantitative real-time polymerase chain reaction. Neutrophil and macrophage infiltration in multiple organs was analyzed by immunohistochemical staining. Leukotriene B4 (LTB4) levels in mouse and human serum samples were determined by enzyme-linked immunosorbent assay.FINDINGS: Transcriptional changes within diverse organs during PanIN and PDAC stages were identified. Using Gene Ontology enrichment analysis, increased neutrophil infiltration was discovered as a central and prominent affected feature, which occurred in the liver, lung, and stomach at the PanIN stage. The brain appeared to be well protected from the sequels of PanIN or PDAC. Importantly, serum LTB4 was able to discriminate PDAC from normal controls, chronic pancreatitis, and intraductal papillary mucinous neoplasms with high performance.INTERPRETATION: Our study provides a high-resolution cartographic view of the dynamic multiorgan transcriptomic landscape of mice with PDAC and its precursor lesions. Our findings suggest that LTB4 could serve as a biomarker for the early detection of PDAC.FUNDING: The complete list of funders can be found in the Acknowledgement section.
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| 23. |
- Li, Gang, et al.
(författare)
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Selective Electrochemical Alkaline Seawater Oxidation Catalyzed by Cobalt Carbonate Hydroxide Nanorod Arrays with Sequential Proton-Electron Transfer Properties
- 2021
-
Ingår i: ACS Sustainable Chemistry and Engineering. - : American Chemical Society (ACS). - 2168-0485. ; 9:2, s. 905-913
-
Tidskriftsartikel (refereegranskat)abstract
- Seawater oxygen evolution is one of the promising energy conversion technologies for large-scale renewable energy storage. It requires efficient catalysts to accelerate the oxygen evolution reaction (OER) for sustained water oxidation, avoiding chlorine evolution under acidic conditions or hypochlorite formation in alkaline solutions. Conventional metal oxide-based OER catalysts follow the adsorbate evolution mechanism that involves concerted proton-electron transfer steps at the active sites. Thus, on the scale of reversible hydrogen electrode, their catalytic activity is independent of the pH of electrolytes. In the present study, nanostructured cobalt carbonate hydroxide (CoCH) with sequential proton-electron transfer properties was tested as a catalyst for seawater oxygen evolution. CoCH exhibited pH-dependent water oxidation activities, thereby providing larger potential and current operating windows for selective water oxidation compared to the catalysts with pH-independent OER activities. The operating window can be further expanded by increasing the pH of the electrolyte.
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| 24. |
- Lin, Wenwei, et al.
(författare)
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Rotation-invariant descriptors learned with circulant convolution neural networks
- 2023
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Ingår i: 2023 IEEE 35th International Conference on Tools with Artificial Intelligence (ICTAI). - : IEEE. - 9798350342734 - 9798350342741 ; , s. 415-422
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Konferensbidrag (refereegranskat)abstract
- Extracting local features for accurate correspondences between image pairs is an essential basis for various computer vision tasks. Recent works have shown that deep neural networks (DNNs) have demonstrated promising performance in challenging environments. However, these state-of-the-art DNN-based approaches are not well suited for the scenario with geometry rotations due to their intrinsic deficiencies of square kernel structure. That is, square kernel structures in standard DNNs cannot fully identify the essentials of the rotations in geometry. To address this problem, we present RICNN, a novel deep learning framework that encodes invariance against the rotations in geometry explicitly into convolutional neural networks. Rather than using the square-shaped kernel structure, RICNN adopts sectorshaped convolutional kernels to achieve encoding invariance in all rotations. With the explicitness of such rotation encoding, RICNN enables the transfer of perspective DNN models to obtain rotation-invariant descriptions. Furthermore, we propose a novel multi-level hinge triplet loss function to strengthen the matching constraints against geometry rotations. Comprehensive experiments demonstrate the strong generalization ability of the RICNN descriptor on the HPatches dataset. Toward the rotation invariance evaluation, our method shows state-of-the-art results.
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| 25. |
- Lindblad, Oscar, et al.
(författare)
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BEX1 acts as a tumor suppressor in acute myeloid leukemia.
- 2015
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Ingår i: Oncotarget. - 1949-2553. ; 6:25, s. 21395-21405
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Tidskriftsartikel (refereegranskat)abstract
- Acute myeloid leukemia (AML) is a heterogeneous disease of the myeloid lineage. About 35% of AML patients carry an oncogenic FLT3 mutant making FLT3 an attractive target for treatment of AML. Major problems in the development of FLT3 inhibitors include lack of specificity, poor response and development of a resistant phenotype upon treatment. Further understanding of FLT3 signaling and discovery of novel regulators will therefore help to determine additional pharmacological targets in FLT3-driven AML. In this report, we identified BEX1 as a novel regulator of oncogenic FLT3-ITD-driven AML. We showed that BEX1 expression was down-regulated in a group of AML patients carrying FLT3-ITD. Loss of BEX1 expression resulted in poor overall survival (hazard ratio, HR = 2.242, p = 0.0011). Overexpression of BEX1 in mouse pro-B and myeloid cells resulted in decreased FLT3-ITD-dependent cell proliferation, colony and tumor formation, and in increased apoptosis in vitro and in vivo. BEX1 localized to the cytosolic compartment of cells and significantly decreased FLT3-ITD-induced AKT phosphorylation without affecting ERK1/2 or STAT5 phosphorylation. Our data suggest that the loss of BEX1 expression in FLT3-ITD driven AML potentiates oncogenic signaling and leads to decreased overall survival of the patients.
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| 26. |
- Liu, Gang, et al.
(författare)
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Plasma Levels of Fatty Acid-Binding Protein 4, Retinol-Binding Protein 4, High-Molecular-Weight Adiponectin, and Cardiovascular Mortality among Men with Type 2 Diabetes : A 22-Year Prospective Study
- 2016
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Ingår i: Arteriosclerosis, Thrombosis, and Vascular Biology. - 1079-5642 .- 1524-4636. ; 36:11, s. 2259-2267
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Tidskriftsartikel (refereegranskat)abstract
- Objective - To examine select adipokines, including fatty acid-binding protein 4, retinol-binding protein 4, and high-molecular-weight (HMW) adiponectin in relation to cardiovascular disease (CVD) mortality among patients with type 2 diabetes mellitus. Approach and Results - Plasma levels of fatty acid-binding protein 4, retinol-binding protein 4, and HMW adiponectin were measured in 950 men with type 2 diabetes mellitus in the Health Professionals Follow-up Study. After an average of 22 years of follow-up (1993-2015), 580 deaths occurred, of whom 220 died of CVD. After multivariate adjustment for covariates, higher levels of fatty acid-binding protein 4 were significantly associated with a higher CVD mortality: comparing extreme tertiles, the hazard ratio and 95% confidence interval of CVD mortality was 1.78 (1.22-2.59; P trend=0.001). A positive association was also observed for HMW adiponectin: the hazard ratio (95% confidence interval) was 2.07 (1.42-3.06; P trend=0.0002), comparing extreme tertiles, whereas higher retinol-binding protein 4 levels were nonsignificantly associated with a decreased CVD mortality with an hazard ratio (95% confidence interval) of 0.73 (0.50-1.07; P trend=0.09). A Mendelian randomization analysis suggested that the causal relationships of HMW adiponectin and retinol-binding protein 4 would be directionally opposite to those observed based on the biomarkers, although none of the Mendelian randomization associations achieved statistical significance. Conclusions - These data suggest that higher levels of fatty acid-binding protein 4 and HMW adiponectin are associated with elevated CVD mortality among men with type 2 diabetes mellitus. Biological mechanisms underlying these observations deserve elucidation, but the associations of HMW adiponectin may partially reflect altered adipose tissue functionality among patients with type 2 diabetes mellitus.
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| 27. |
- Liu, Jian, et al.
(författare)
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Amphipathic Side Chain of a Conjugated Polymer Optimizes Dopant Location toward Efficient N-Type Organic Thermoelectrics
- 2021
-
Ingår i: Advanced Materials. - : WILEY-V C H VERLAG GMBH. - 0935-9648 .- 1521-4095. ; 33
-
Tidskriftsartikel (refereegranskat)abstract
- There is no molecular strategy for selectively increasing the Seebeck coefficient without reducing the electrical conductivity for organic thermoelectrics. Here, it is reported that the use of amphipathic side chains in an n-type donor-acceptor copolymer can selectively increase the Seebeck coefficient and thus increase the power factor by a factor of approximate to 5. The amphipathic side chain contains an alkyl chain segment as a spacer between the polymer backbone and an ethylene glycol type chain segment. The use of this alkyl spacer does not only reduce the energetic disorder in the conjugated polymer film but can also properly control the dopant sites away from the backbone, which minimizes the adverse influence of counterions. As confirmed by kinetic Monte Carlo simulations with the host-dopant distance as the only variable, a reduced Coulombic interaction resulting from a larger host-dopant distance contributes to a higher Seebeck coefficient for a given electrical conductivity. Finally, an optimized power factor of 18 mu W m(-1) K-2 is achieved in the doped polymer film. This work provides a facile molecular strategy for selectively improving the Seebeck coefficient and opens up a new route for optimizing the dopant location toward realizing better n-type polymeric thermoelectrics.
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| 28. |
- Liu, Tao, et al.
(författare)
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16% efficiency all-polymer organic solar cells enabled by a finely tuned morphology via the design of ternary blend
- 2021
-
Ingår i: Joule. - : CELL PRESS. - 2542-4351. ; 5:4, s. 914-930
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Tidskriftsartikel (refereegranskat)abstract
- A SUMMARY There is an urgent demand for all-polymer organic solar cells (AP-OSCs) to gain higher efficiency. Here, we successfully improve the performance to 16.09% by introducing a small amount of BN-T, a B <- N-type polymer acceptor, into the PM6:PY-IT blend. It has been found that BN-T makes the active layer, based on the PM6:PY-IT:BN-T ternary blend, more crystalline but meanwhile slightly reduces the phase separation, leading to enhancement of both exciton harvesting and charge transport. From a thermodynamic viewpoint, BN-T prefers to reside between PM6 and PY-IT, and the fraction of this fine-tunes the morphology. Besides, a significantly reduced nonradiative energy loss occurs in the ternary blend, along with the coexistence of energy and charge transfer between the two acceptors. The progressive performance facilitated by these improved properties demonstrates that AP-OSCs can possibly comparably efficient with those based on small molecule acceptors, further enhancing the competitiveness of this device type.
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| 29. |
- Nie, Huizhen, et al.
(författare)
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The short isoform of PRLR suppresses the pentose phosphate pathway and nucleotide synthesis through the NEK9-Hippo axis in pancreatic cancer
- 2021
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Ingår i: Theranostics. - : Ivyspring International Publisher. - 1838-7640. ; 11:8, s. 3898-3915
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Tidskriftsartikel (refereegranskat)abstract
- Prolactin binding to the prolactin receptor exerts pleiotropic biological effects in vertebrates. The prolactin receptor (PRLR) has multiple isoforms due to alternative splicing. The biological roles and related signaling of the long isoform (PRLR-LF) have been fully elucidated. However, little is known about the short isoform (PRLR-SF), particularly in cancer development and metabolic reprogramming, a core hallmark of cancer. Here, we reveal the role and underlying mechanism of PRLR-SF in pancreatic ductal adenocarcinoma (PDAC). Methods: A human PDAC tissue array was used to investigate the clinical relevance of PRLR in PDAC. The in vivo implications of PRLR-SF in PDAC were examined in a subcutaneous xenograft model and an orthotopic xenograft model. Immunohistochemistry was performed on tumor tissue obtained from genetically engineered KPC (KrasG12D/+; Trp53R172H/+; Pdx1-Cre) mice with spontaneous tumors. 13C-labeled metabolite measures, LC-MS, EdU incorporation assays and seahorse analyses were used to identify the effects of PRLR-SF on the pentose phosphate pathway and glycolysis. We identified the molecular mechanisms by immunofluorescence, coimmunoprecipitation, proximity ligation assays, chromatin immunoprecipitation and promoter luciferase activity. Public databases (TCGA, GEO and GTEx) were used to analyze the expression and survival correlations of the related genes. Results: We demonstrated that PRLR-SF is predominantly expressed in spontaneously forming pancreatic tumors of genetically engineered KPC mice and human PDAC cell lines. PRLR-SF inhibits the proliferation of PDAC cells (AsPC-1 and BxPC-3) in vitro and tumor growth in vivo. We showed that PRLR-SF reduces the expression of genes in the pentose phosphate pathway (PPP) and nucleotide biosynthesis by activating Hippo signaling. TEAD1, a downstream transcription factor of Hippo signaling, directly regulates the expression of G6PD and TKT, which are PPP rate-limiting enzymes. Moreover, NEK9 directly interacts with PRLR-SF and is the intermediator between PRLR and the Hippo pathway. The PRLR expression level is negatively correlated with overall survival and TNM stage in PDAC patients. Additionally, pregnancy and lactation increase the ratio of PRLR-SF:PRLR-LF in the pancreas of wild-type mice and subcutaneous PDAC xenograft tumors. Conclusion: Our characterization of the relationship between PRLR-SF signaling, the NEK9-Hippo pathway, PPP and nucleotide synthesis explains a mechanism for the correlation between PRLR-SF and metabolic reprogramming in PDAC progression. Strategies to alter this pathway might be developed for the treatment or prevention of pancreatic cancer.
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| 30. |
- Park, Jong-Sun, et al.
(författare)
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Transpolar Arcs During a Prolonged Radial Interplanetary Magnetic Field Interval
- 2021
-
Ingår i: Journal of Geophysical Research - Space Physics. - : John Wiley & Sons. - 2169-9380 .- 2169-9402. ; 126:6
-
Tidskriftsartikel (refereegranskat)abstract
- Transpolar arcs (TPAs) are believed to predominantly occur under northward interplanetary magnetic field (IMF) conditions with their hemispheric asymmetry controlled by the Sun-Earth (radial) component of the IMF. In this study, we present observations of TPAs that appear in both the northern and southern hemispheres even during a prolonged interval of radially oriented IMF. The Defense Meteorological Satellite Program (DMSP) F16 and the Thermosphere Ionosphere Mesosphere Energetics and Dynamics (TIMED) satellites observed TPAs on the dawnside polar cap in both hemispheres (one TPA structure in the southern hemisphere and two in the northern hemisphere) during an interval of nearly earthward-oriented IMF on October 29, 2005. The southern hemisphere TPA and one of the northern hemisphere TPAs are associated with electron and ion precipitation and mostly sunward plasma flow (with shears) relative to their surroundings. Meanwhile, the other TPA in the northern hemisphere is associated with an electron-only precipitation and antisunward flow relative to its surroundings. Our observations indicate the following: (a) the TPA formation is not limited to northward IMF conditions; (b) the TPAs can be located on both closed field lines rooted in the polar cap of both hemispheres and open field lines connected to the northward field lines draped over one hemisphere of the magnetopause. We believe that the TPAs presented here are the result of both indirect and direct processes of solar wind energy transfer to the high-latitude ionosphere.
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| 31. |
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| 32. |
- Qiao, Sibo, et al.
(författare)
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DPC-MSGATNet : dual-path chain multi-scale gated axial-transformer network for four-chamber view segmentation in fetal echocardiography
- 2023
-
Ingår i: Complex & Intelligent Systems. - : Springer Nature. - 2199-4536 .- 2198-6053. ; :9, s. 4503-4519
-
Tidskriftsartikel (refereegranskat)abstract
- Echocardiography is essential in evaluating fetal cardiac anatomical structures and functions when clinicians conduct early treatment and screening for congenital heart defects, a common and intricate fetal malformation. Nevertheless, the prenatal detection rate of fetal CHD remains low since the peculiarities of fetal cardiac structures and the variousness of fetal CHD. Precisely segmenting four cardiac chambers can assist clinicians in analyzing cardiac morphology and further facilitate CHD diagnosis. Hence, we design a dual-path chain multi-scale gated axial-transformer network (DPC-MSGATNet) that simultaneously models global dependencies and local visual cues for fetal ultrasound (US) four-chamber (FC) views and further accurately segments four chambers. Our DPC-MSGATNet includes a global and a local branch that simultaneously operates on an entire FC view and image patches to learn multi-scale representations. We design a plug-and-play module, Interactive dual-path chain gated axial-transformer (IDPCGAT), to enhance the interactions between global and local branches. In IDPCGAT, the multi-scale representations from the two branches can complement each other, capturing the same region’s salient features and suppressing feature responses to maintain only the activations associated with specific targets. Extensive experiments demonstrate that the DPC-MSGATNet exceeds seven state-of-the-art convolution- and transformer-based methods by a large margin in terms of F1 and IoU scores on our fetal FC view dataset, achieving a F1 score of 96.87% and an IoU score of 93.99%. The codes and datasets can be available at https://github.comQiaoSiBo/DPC-MSGATNet.
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| 33. |
- Qiao, Sibo, et al.
(författare)
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SPReCHD : Four-Chamber Semantic Parsing Network for Recognizing Fetal Congenital Heart Disease in Medical Metaverse
- 2024
-
Ingår i: IEEE journal of biomedical and health informatics. - : Institute of Electrical and Electronics Engineers (IEEE). - 2168-2194 .- 2168-2208. ; 28:6, s. 3672-3682
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Tidskriftsartikel (refereegranskat)abstract
- Echocardiography is essential for evaluating cardiac anatomy and function during early recognition and screening for congenital heart disease (CHD), a widespread and complex congenital malformation. However, fetal CHD recognition still faces many difficulties due to instinctive fetal movements, artifacts in ultrasound images, and distinctive fetal cardiac structures. These factors hinder capturing robust and discriminative representations from ultrasound images, resulting in CHD's low prenatal detection rate. Hence, we propose a multi-scale gated axial-transformer network (MSGATNet) to capture fetal four-chamber semantic information. Then, we propose a SPReCHD: four-chamber semantic parsing network for recognizing fetal CHD in the clinical treatment of the medical metaverse, integrating MSGATNet to segment and locate four-chamber arbitrary contours, further capturing distinguished representations for the fetal heart. Comprehensive experiments indicate that our SPReCHD is sufficient in recognizing fetal CHD, achieving a precision of 95.92%, a recall of 94%, an accuracy of 95%, and a F1 score of 94.95% on the test set, dramatically improving the fetal CHD's prenatal detection rate.
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| 34. |
- Qiu, Chun-Yu, et al.
(författare)
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Revealing the concentration of hydrogen peroxide in fuel cell catalyst layers by an in-operando approach
- 2022
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Ingår i: Chinese Journal of Catalysis. - 1872-2067. ; 43:7, s. 1918-1926
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Tidskriftsartikel (refereegranskat)abstract
- To evaluate the H2O2-tolerance of non-Pt oxygen reduction reaction (ORR) catalysts as well as investigate the H2O2-induced decay mechanism, the selection of an appropriate H2O2 concentration is a prerequisite. However, the concentration criterion is still unclear because of the lack of in-operando methods to determine the actual concentration of H2O2 in fuel cell catalyst layers. In this work, an electrochemical probe method was successfully established to in-operando monitor the H2O2 in non-Pt catalyst layers for the first time. The local concentration of H2O2 was revealed to reach 17 mmol/L, which is one order of magnitude higher than that under aqueous electrodes test conditions. Powered by the new knowledge, a concentration criterion of at least 17 mmol/L is suggested. This work fills in the large gap between aqueous electrode tests and the real fuel cell working conditions, and highlights the importance of in-operando monitoring methods.
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| 35. |
- Shen, Qian, et al.
(författare)
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The Genome of Artemisia annua Provides Insight into the Evolution of Asteraceae Family and Artemisinin Biosynthesis
- 2018
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Ingår i: Molecular Plant. - : Cell Press. - 1674-2052 .- 1752-9867. ; 11:6, s. 776-788
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Tidskriftsartikel (refereegranskat)abstract
- Artemisia annua, commonly known as sweet wormwood or Qinghao, is a shrub native to China and has long been used for medicinal purposes. A. annua is now cultivated globally as the only natural source of a potent anti-malarial compound, artemisinin. Here, we report a high-quality draft assembly of the 1.74-gigabase genome of A. annua, which is highly heterozygous, rich in repetitive sequences, and contains 63 226 protein-coding genes, one of the largest numbers among the sequenced plant species. We found that, as one of a few sequenced genomes in the Asteraceae, the A. annua genome contains a large number of genes specific to this large angiosperm clade. Notably, the expansion and functional diversification of genes encoding enzymes involved in terpene biosynthesis are consistent with the evolution of the artemisinin biosynthetic pathway. We further revealed by transcriptome profiling that A. annua has evolved the sophisticated transcriptional regulatory networks underlying artemisinin biosynthesis. Based on comprehensive genomic and transcriptomic analyses we generated transgenic A. annua lines producing high levels of artemisinin, which are now ready for large-scale production and thereby will help meet the challenge of increasing global demand of artemisinin.
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| 36. |
- Shen, Xiao-Gang, et al.
(författare)
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Downregulation of caspase-10 predicting poor survival after resection of stage II colorectal cancer
- 2011
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Ingår i: International Journal of Colorectal Disease. - : Springer Verlag (Germany). - 0179-1958 .- 1432-1262. ; 26:12, s. 1519-1524
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Tidskriftsartikel (refereegranskat)abstract
- Purpose The aim of this study was to evaluate the prevalence and clinical significance of caspase-10 mRNA expression in stage II colorectal cancer. less thanbrgreater than less thanbrgreater thanMethods Quantitative real-time reverse transcription-polymerase chain reaction (RT-PCR) was used to analyze caspase-10 expression in cancer tissue and corresponding normal mucosa from 120 patients with stage II colorectal cancer. Variables were analyzed by Chi-square test or Fishers exact test. Survival was evaluated with method of Kaplan-Meier. Multivariate analysis was performed with Coxs proportional hazards model. less thanbrgreater than less thanbrgreater thanResults The expression of caspase-10 mRNA was found to be downregulated in cancer tissue compared to normal mucosa (P = 0.001). Poorly differentiated cancer showed lower mRNA expression than cancer with greater differentiation (P = 0.031). Univariate survival curves, estimated using the method of Kaplan-Meier, defined a significant association between caspase-10 expression and both overall survival (P = 0.012) and disease-free survival (P = 0.021). A multivariate analysis, performed by Coxs proportional hazards regression model, confirmed that a low caspase-10 expression was the only significant factor to predict poor prognosis in patients with stage II colorectal cancer. less thanbrgreater than less thanbrgreater thanConclusion Our data indicate that caspase-10 expression, measured by quantitative real-time RT-PCR, is a possible prognostic factor in patients with stage II colorectal cancer.
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| 37. |
- Shi, Shengwei, et al.
(författare)
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11,11,12,12-Tetracyanonaphtho-2,6-quinodimethane in Contact with Ferromagnetic Electrodes for Organic Spintronics
- 2018
-
Ingår i: Advanced Electronic Materials. - : WILEY. - 2199-160X. ; 4:7
-
Tidskriftsartikel (refereegranskat)abstract
- Spinterface engineering has shown quite important roles in organic spintronics as it can improve spin injection or extraction. In this study, 11,11,12,12-tetracyanonaptho-2,6-quinodimethane (TNAP) is introduced as an interfacial layer for a prototype interface of Fe/TNAP. An element-specific investigation of the electronic and magnetic structures of Fe/TNAP system by use of near edge X-Ray absorption fine structure (NEXAFS) and X-ray magnetic circular dichroism (XMCD) is reported. Strong hybridization between TNAP and Fe and induced magnetization of N atoms in TNAP molecule are observed. XMCD sum rule analysis demonstrates that the adsorption of TNAP reduces the spin moment of Fe by 12%. In addition, induced magnetization in N K-edge of TNAP is also found with other commonly used ferromagnets in organic spintronics, such as La0.7Sr0.3MnO3 and permalloy, which makes TNAP a very promising molecule for spinterface engineering in organic spintronics.
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| 38. |
- Song, Yimin, et al.
(författare)
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A novel five-degree-of-freedom parallel manipulator and its kinematic optimization
- 2014
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Ingår i: Journal of Mechanisms and Robotics. - : ASME Press. - 1942-4302 .- 1942-4310. ; 6:4
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Tidskriftsartikel (refereegranskat)abstract
- Driven by requirements of five-axis numerical control (NC) machine for its executive mechanism, this paper creatively proposes a flow path to synthesize a novel class of n-degree-of-freedom (n-DoF, 4 <= n <= 6) parallel manipulators (PMs) resorting to four steps, and takes a patented 5-DoF PM, named T5, for example to demonstrate the flow path in depth. Comparing with existing five-axis executive mechanisms, this novel class of the PMs has some advantages of light end-effector, good static, dynamic performance, and so on. Upon the underlying architecture of T5, the kinematic analysis and optimal design are carried out for the first time, in which two essential procedures are involved, one is the kinematic performance index by means of the reciprocal product associated with the wrench screw and twist screw with specific physical meaning, the other is the design method adopted to perform the multi-objective dimensional synthesis using an artificial intelligence approach, that is nondominated sorting genetic algorithm II (NSGA-II). This paper is aimed at laying a solid theoretical and technical foundation for the prototype design and manufacture of T5 PM.
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| 39. |
- Song, Yimin, et al.
(författare)
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Elasto-dynamic analysis of a novel 2-DoF rotational parallel mechanism with an articulated travelling platform
- 2016
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Ingår i: Meccanica (Milano. Print). - : Springer Netherlands. - 0025-6455 .- 1572-9648. ; 51:7, s. 1547-1557
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Tidskriftsartikel (refereegranskat)abstract
- This paper carries out the elasto-dynamic analysis of a novel 2 degrees-of-freedom (DoF) rotational parallel mechanism (RPM) with an articulated travelling platform by means of kineto-elasto dynamic method. The architecture of the proposed 2-DoF RPM is firstly described, and then its kinematic analysis is carried out by closed-loop vector method. On the basis of finite element analysis, the elasto-dynamic models of movable components are established before assembling to formulate the elasto-dynamic equations of the whole mechanism in the light of deformation compatibility conditions. The free vibration equation is then achieved to evaluate the natural frequency of the novel 2-DoF RPM. Finally, an example is illustrated and the results are verified by finite element software. It shows that the relatively high natural frequencies and good dynamic performance make the novel 2-DoF RPM a promising solution for pose-adjusting module of 5-DoF machine centre.
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| 40. |
- Song, Yimin, et al.
(författare)
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Kinematic analysisand optimal design of a novel 1T3R parallel manipulator with an articulated travelling plate
- 2014
-
Ingår i: Robotics and Computer-Integrated Manufacturing. - : Elsevier. - 0736-5845 .- 1879-2537. ; 30:5, s. 508-516
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Tidskriftsartikel (refereegranskat)abstract
- Driven by the requirements of the large-scale component assemblage for the docking platform, this paper proposes a novel one-translational-three-rotational (1T3R) parallel manipulator with an articulated travelling plate, which can provide high stiffness and good accuracy performances in the assemblage. The underlying architecture of this manipulator is briefly addressed with emphasis on the practical realization of the articulated travelling plate. On the basis of the kinematic analysis of the 1T3R parallel manipulator, its optimal design considering the force and motion transmissibility is carried out, in which the generalized virtual power transmissibility of this manipulator is defined. This paper aims at laying a solid theoretical and technical foundation for the prototype design and manufacture of the 1T3R parallel manipulator.
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| 41. |
- Sun, Gang, et al.
(författare)
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Energy-efficient and traffic-aware service function chaining orchestration in multi-domain networks
- 2019
-
Ingår i: Future generations computer systems. - : Elsevier. - 0167-739X .- 1872-7115. ; 91, s. 347-360
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Tidskriftsartikel (refereegranskat)abstract
- Service function chaining (SFC) provisioning is helpful not only for saving the capital expenditure (CAPEX) and operational expenditure (OPEX) of a network provider but also for reducing energy consumption in the substrate network. However, to the best of our knowledge, there has been little research on the problem of energy consumption for orchestrating online SFC requests in multi-domain networks. In this paper, we first formulate the problem of an energy-efficient online SFC request that is orchestrated across multiple clouds as an integer linear programming (ILP) model to find an optimal solution. Then, we analyze the complexity of this ILP model and prove that the problem is NP-hard. Additionally, we propose a low-complexity heuristic algorithm named energy-efficient online SFC request orchestration across multiple domains (EE-SFCO-MD) for near-optimally solving the mentioned problem. Finally, we conduct simulation experiments to evaluate the performance of our algorithm. Simulation results show that EE-SFCO-MD consumes less energy than existing approaches while the online SFC’s requirements are met and the privacy of each cloud is effectively guaranteed. The low computational complexity of the heuristic approach makes it applicable for quickly responding to online SFC requests.
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| 42. |
- Sun, Gang, et al.
(författare)
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Low-latency and Resource-efficient Service Function Chaining Orchestration in Network Function Virtualization
- 2020
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Ingår i: IEEE Internet of Things Journal. - : IEEE. - 2327-4662. ; 7:7, s. 5760-5772
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Tidskriftsartikel (refereegranskat)abstract
- Recently, network function virtualization (NFV) has been proposed to solve the dilemma faced by traditional networks and to improve network performance through hardware and software decoupling. The deployment of the service function chain (SFC) is a key technology that affects the performance of virtual network function (VNF). The key issue in the deployment of SFCs is proposing effective algorithms to achieve efficient use of resources. In this paper, we propose a service function chain deployment optimization (SFCDO) algorithm based on a breadth-first search (BFS). The algorithm first uses a BFS based algorithm to find the shortest path between the source node and the destination node. Then, based on the shortest path, the path with the fewest hops is preferentially chosen to implement the SFC deployment. Finally, we compare the performances with the greedy and simulated annealing (G-SA) algorithm. The experiment results show that the proposed algorithm is optimized in terms of end-to-end delay and bandwidth resource consumption. In addition, we also consider the load rate of the nodes to achieve network load balancing.
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| 43. |
- Sun, Tao, et al.
(författare)
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Optimal design of a parallel mechanism with three rotational degrees of freedom
- 2012
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Ingår i: Robotics and Computer-Integrated Manufacturing. - : Elsevier BV. - 0736-5845 .- 1879-2537. ; 28:4, s. 500-508
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Tidskriftsartikel (refereegranskat)abstract
- This paper presents the concept design of a pose-adjustment system applied in the large fuselage or wing assembly of aircraft manufacturing which including a 3-degree-of-freedom rotational parallel mechanism (3-DoFs RPM), pogo columns and three tracks. The optimal design of the 3-DoFs RPM with its topology a 3-PUS&S mechanism is detailed, which is designed as a rigid yet compact module that can act as a pose-adjustment mechanism moving along three long tracks for large aircraft structural component assembly, a middle fuselage for example. Inverse kinematics of the 3-DoFs RPM with the exponential product method is achieved to lay the foundation for its kinematic synthesis. Next, with the commercial mathematical software, one can get the reachable workspace and define the prescribed workspace, respectively. Then, dimensional synthesis of the 3-DoFs RPM is executed to achieve a relatively good kinematic performance within its workspace. With the commercial CAE software, stiffness analysis is carried out for performance evaluation of the 3-DoFs RPM virtual prototype.
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| 44. |
- Sun, Xiao-dong, et al.
(författare)
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Implementing a novel capture and ligation probe-PCR method in mass screen and treatment to support malaria elimination efforts in the China-Myanmar border region
- 2023
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Ingår i: Malaria Journal. - : BioMed Central (BMC). - 1475-2875. ; 22:1
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Tidskriftsartikel (refereegranskat)abstract
- BackgroundMass screening and treatment (MSAT) for malaria elimination lacks an ideal diagnostic tool to allow sensitive and affordable test of the target population in the field. This study evaluated whether Capture and Ligation Probe-PCR (CLIP-PCR) could be used in a field MSAT in Laiza City, Myanmar.MethodsOn day 0, two dried blood spots were collected from each participant. On day 1, all samples were screened for Plasmodium in a 20 m(2) laboratory with workbench, a biosafety cabinet, a refrigerator, a benchtop shaking incubator and a qPCR machine, by four technicians using CLIP-PCR with sample pooling, at a health clinic of the Chinese bordering town of Nabang. On day 2, all positives were followed up and treated.ResultsOf 15,038 persons (65% of the total population) screened, 204 (1.36%) were CLIP-PCR positives. Among them, 188, 14, and 2 were infected with Plasmodium vivax, Plasmodium falciparum, and P. vivax/P. falciparum mix, respectively. The testing capacity was 538 persons/day, with a cost of US$0.92 /person. The proportion of submicroscopic infection was 64.7%. All positive individuals received treatment within 72 h after blood collection.ConclusionUsing CLIP-PCR in MSAT in low transmission settings can support the malaria elimination efforts in the China-Myanmar border region.
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| 45. |
- Sun, Xin, et al.
(författare)
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Reducing supply risk of critical materials for clean energy via foreign direct investment
- 2024
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Ingår i: Nature Sustainability. - : Springer Nature. - 2398-9629. ; 7:5, s. 672-681
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Tidskriftsartikel (refereegranskat)abstract
- Existing research on the security of the supply of critical materials for clean energy generally aggregates information at the country level, a practice that obscures the extensive role of foreign direct investment (FDI) in the production of critical materials. FDI refers to an ownership stake in a company or project by an overseas investor. Here we establish a database for global mining of lithium, cobalt, nickel and platinum at company level, covering 240 countries and regions. We show that 47% of lithium, 71% of cobalt, 41% of nickel and 34% of platinum mined in 2019 were under FDI. We then explore how FDI may affect supply risks by proposing a supply risk index that allocates production of the critical materials to the country of origin of investors instead of the country where production is located. We present upper and lower bounds of the supply risk index that reflect scenarios where either all investors or only state investors prioritize the home-country demand, respectively. This study presents an approach for assessing the national supply risks of critical materials, considering the geographical allocation of FDI.
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| 46. |
- Wan, Liyang, et al.
(författare)
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Molecular Degradation of Iron Phthalocyanine during the Oxygen Reduction Reaction in Acidic Media
- 2022
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Ingår i: ACS Catalysis. - : American Chemical Society (ACS). - 2155-5435. ; 12:18, s. 11097-11107
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Tidskriftsartikel (refereegranskat)abstract
- Molecular iron phthalocyanine (FePc) possesses an FeN4 active site structure similar to practical pyrolyzed Fe/N/C catalysts for the acidic oxygen reduction reaction (ORR), making it an ideal model system to derive the degradation mechanism of such catalysts. However, the degradation mechanism of FePc during the acidic ORR has been largely unclear to date. Herein, five most likely degradation factors affecting FePc-based ORR activity are individually investigated and compared. The attack by free radicals is found to be the main reason for the instability of FePc. Assisted by the combination of several spectroscopic methods, we successfully identify the degradation products and then propose a full structural evolution of molecular FePc degradation. Finally, high similarity in the decay mechanism between molecular FePc and practical Fe/N/C catalysts was present. This study provides a clear picture of the currently missing degradation mechanism of molecular FePc during acidic ORR, which will assist future investigations on the performance degradation of practical Fe/N/C catalysts.
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| 47. |
- Wang, Suhao, et al.
(författare)
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A Chemically Doped Naphthalenediimide-Bithiazole Polymer for n-Type Organic Thermoelectrics
- 2018
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Ingår i: Advanced Materials. - : WILEY-V C H VERLAG GMBH. - 0935-9648 .- 1521-4095. ; 30:31
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Tidskriftsartikel (refereegranskat)abstract
- The synthesis of a novel naphthalenediimide (NDI)-bithiazole (Tz2)-based polymer [P(NDI2OD-Tz2)] is reported, and structural, thin-film morphological, as well as charge transport and thermoelectric properties are compared to the parent and widely investigated NDI-bithiophene (T2) polymer [P(NDI2OD-T2)]. Since the steric repulsions in Tz2 are far lower than in T2, P(NDI2OD-Tz2) exhibits a more planar and rigid backbone, enhancing p-p chain stacking and intermolecular interactions. In addition, the electron-deficient nature of Tz2 enhances the polymer electron affinity, thus reducing the polymer donor-acceptor character. When n-doped with amines, P(NDI2OD-Tz2) achieves electrical conductivity (approximate to 0.1 S cm(-1)) and a power factor (1.5 mu W m(-1) K-2) far greater than those of P(NDI2OD-T2) (0.003 S cm(-1) and 0.012 mu W m(-1) K-2, respectively). These results demonstrate that planarized NDI-based polymers with reduced donor-acceptor character can achieve substantial electrical conductivity and thermoelectric response.
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| 48. |
- Wu, Lei, et al.
(författare)
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Limited Song Mixing Without Genomic Gene Flow in a Contact Zone Between Two Songbird Species
- 2023
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Ingår i: Molecular biology and evolution. - : Oxford University Press. - 0737-4038 .- 1537-1719. ; 40:3
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Tidskriftsartikel (refereegranskat)abstract
- Song is considered to play an important role in the maintenance of prezygotic reproductive isolation between closely related songbird species. Therefore, song mixing in a contact zone between closely related species is often considered as evidence of hybridization. The Sichuan Leaf Warbler Phylloscopus forresti and the Gansu Leaf Warbler Phylloscopus kansuensis, which diverged 2 million years ago, have formed a contact zone in the south of the Gansu Province of China, where mixed songs have been observed. In this study, we investigated the potential causes and consequences of song mixing by integrating bioacoustic, morphological, mitochondrial, and genomic data with field ecological observations. We found that the two species display no apparent morphological differences, whereas their songs differ dramatically. We demonstrated that ∼11% of the males in the contact zone sang mixed songs. Two males singing mixed song were genotyped, and both were found to be P. kansuensis. Despite the presence of mixed singers, population genomic analyses detected no signs of recent gene flow between the two species, although two possible cases of mitochondrial introgression were identified. We conclude that the rather limited song mixing does not lead to, or result from, hybridization, and hence does not result in the breakdown of reproductive barriers between these cryptic species.
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| 49. |
- Xiao, Chao, et al.
(författare)
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RBBP6 increases radioresistance and serves as a therapeutic target for preoperative radiotherapy in colorectal cancer
- 2018
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Ingår i: Cancer Science. - : Blackwell Publishing. - 1347-9032 .- 1349-7006. ; 109:4, s. 1075-1087
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Tidskriftsartikel (refereegranskat)abstract
- Radiotherapy (RT) can be used as preoperative treatment to downstage initially unresectable locally rectal carcinoma, but the radioresistance and recurrence remain significant problems. Retinoblastoma binding protein 6 (RBBP6) has been implicated in the regulation of cell cycle, apoptosis and chemoresistance both in vitro and in vivo. This study investigated whether the inhibition of RBBP6 expression would improve radiosensitivity in human colorectal cancer cells. After SW620 and HT29 cells were exposed to radiation, the levels of RBBP6 mRNA and protein increased over time in both two cells. Moreover, a significant reduction in clonogenic survival and a decrease in cell viability in parallel with an obvious increase in cell apoptosis were demonstrated in irradiated RBBP6-knockdown cells. Besides, transfection with RBBP6 shRNA improved levels of G2-M phase arrest which blocked the cells in a more radiosensitive period of the cell cycle. These observations indicated that cell cycle and apoptosis mechanisms may be connected with tumor cell survival following radiotherapy. In vivo, tumor growth rate of nude mice in RBBP6-knockdown group was significantly slower than that in other groups. These results indicated that RBBP6 overexpression could resist colorectal cancer cells against radiation by regulating cell cycle and apoptosis pathways, and inhibition of RBBP6 could enhance radiosensitivity of human colorectal cancer.
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| 50. |
- Xing, Yi, et al.
(författare)
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Integrated opposite charge grafting induced ionic-junction fiber
- 2023
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Ingår i: Nature Communications. - : NATURE PORTFOLIO. - 2041-1723. ; 14:1
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Tidskriftsartikel (refereegranskat)abstract
- The emergence of ionic-junction devices has attracted growing interests due to the potential of serving as signal transmission and translation media between electronic devices and biological systems using ions. Among them, fiber-shaped iontronics possesses a great advantage in implantable applications owing to the unique one-dimensional geometry. However, fabricating stable ionic-junction on curved surfaces remains a challenge. Here, we developed a polyelectrolyte based ionic-junction fiber via an integrated opposite charge grafting method capable of large-scale continuous fabrication. The ionic-junction fibers can be integrated into functions such as ionic diodes and ionic bipolar junction transistors, where rectification and switching of input signals are implemented. Moreover, synaptic functionality has also been demonstrated by utilizing the fiber memory capacitance. The connection between the ionic-junction fiber and sciatic nerves of the mouse simulating end-to-side anastomosis is further performed to realize effective nerve signal conduction, verifying the capability for next-generation artificial neural pathways in implantable bioelectronics. Ionic-junction devices are difficult to integrate with fiber-shaped tissues like nerves and muscles for applications in implantable bioelectronics due to their large size and bulk structure. Authors realize here easy to implant fiber-shaped iontronics through an integrated opposite charge grafting process, enabling the construction of ionic logic gates and artificial neural pathways.
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