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- Robinson, N. A., et al.
(författare)
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Applying genetic technologies to combat infectious diseases in aquaculture
- 2022
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Ingår i: Reviews in Aquaculture. - : Wiley. - 1753-5123 .- 1753-5131. ; 15:2, s. 491-535
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Tidskriftsartikel (refereegranskat)abstract
- Disease and parasitism cause major welfare, environmental and economic concerns for global aquaculture. In this review, we examine the status and potential of technologies that exploit genetic variation in host resistance to tackle this problem. We argue that there is an urgent need to improve understanding of the genetic mechanisms involved, leading to the development of tools that can be applied to boost host resistance and reduce the disease burden. We draw on two pressing global disease problems as case studies—sea lice infestations in salmonids and white spot syndrome in shrimp. We review how the latest genetic technologies can be capitalised upon to determine the mechanisms underlying inter- and intra-species variation in pathogen/parasite resistance, and how the derived knowledge could be applied to boost disease resistance using selective breeding, gene editing and/or with targeted feed treatments and vaccines. Gene editing brings novel opportunities, but also implementation and dissemination challenges, and necessitates new protocols to integrate the technology into aquaculture breeding programmes. There is also an ongoing need to minimise risks of disease agents evolving to overcome genetic improvements to host resistance, and insights from epidemiological and evolutionary models of pathogen infestation in wild and cultured host populations are explored. Ethical issues around the different approaches for achieving genetic resistance are discussed. Application of genetic technologies and approaches has potential to improve fundamental knowledge of mechanisms affecting genetic resistance and provide effective pathways for implementation that could lead to more resistant aquaculture stocks, transforming global aquaculture. © 2022 The Authors. Reviews in Aquaculture published by John Wiley & Sons Australia, Ltd.
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- Sveen, Kari Anne, et al.
(författare)
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High levels of autoantibodies against apoB100 p210 are associated with lower incidence of atrial fibrillation in women
- 2022
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Ingår i: Journal of Internal Medicine. - : Wiley. - 0954-6820 .- 1365-2796. ; 291:2, s. 207-217
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Tidskriftsartikel (refereegranskat)abstract
- Background and objectives Atrial fibrillation (AF) is associated with inflammation systemically and in the atrial tissue. Oxidized low-density lipoprotein (LDL) is increased in patients with AF and is suggested to be one of the molecules that drives inflammation. Autoantibodies against oxidized LDL and apolipoprotein B100, the protein component of LDL, are linked to atherosclerotic disease. However, whether these autoantibodies are associated with occurrence of AF is not known. We investigated autoantibodies against oxidized apolipoprotein B100 peptides and incidence of AF in a large population-based cohort. Methods IgM and IgG against native and aldehyde-modified apoB100 peptides 210 (p210) and 45 were analyzed by enzyme-linked immunosorbent assay (ELISA) in 5169 individuals from the Malmo Diet and Cancer cohort. Results Seven hundred sixty-nine incident AF cases were recorded during a follow-up of 21.3 years. Individuals with high levels of IgM against native p210 at baseline had a lower risk to develop AF; however, the association did not remain after adjustment for age and sex. Women had higher levels of IgM against native p210 than men (0.70 +/- 0.22 AU vs. 0.63 +/- 0.21 AU, p < 0.001). The association of IgM against native p210 and AF was significantly different between sexes (p for interaction = 0.024), where females with high IgM against p210 had a lower risk for incidence of AF (hazard ratio [95% confidence interval] 4th versus 1st quartile: 0.67 [0.49-0.91]; p = 0.01) after adjusting for risk factors and comorbidities. Conclusion These findings support an association of humoral autoimmunity with AF.
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- Sveen, Susanne, et al.
(författare)
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Scratch adhesion characteristics of PVD TiAlN deposited on high speed steel, cemented carbide and PCBN substrates
- 2013
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Ingår i: Wear. - : Elsevier. - 0043-1648 .- 1873-2577. ; 308:1-2, s. 133-141
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Tidskriftsartikel (refereegranskat)abstract
- Modern tool materials, ranging from powder metallurgical high speed steel to super hard materials such as polycrystalline cubic boron nitride and diamond, are used as cutting tools in the metal cutting industry. In order to further improve the cutting performance, these tools are frequently coated by thin, hard PVD coatings such as TiN, TiAlN, AlCrO3, etc. In order to develop and design new PVD coatings it is important to characterize the mechanical properties of the coatings and understand the coating/substrate deformation mechanisms in a tribological contact, e.g. metal cutting. For example, it is important to be aware that the mechanical properties of the substrate (tool material) have a significant impact on the practical coating adhesion and the coating failure mechanisms.In the present study scratch testing has been used in order to evaluate to increase the understanding of the mechanical response and potential coating failure modes of cathodic arc evaporated TiAlN deposited on high speed steel, cemented carbide and polycrystalline cubic boron nitride. Post-test characterization of the scratched samples using optical profilometry, scanning electron microscopy and energy dispersive X-ray spectroscopy were performed and the cohesive and adhesive surface failure mechanisms are described and related to the substrate material properties. The results clearly show that, although all substrate materials can be regarded as hard, they result in completely different coating failure mechanisms at the normal load corresponding to substrate exposure. Also, coating failure resulting in substrate exposure does not necessarily correspond to interfacial cracking resulting in adhesive fracture along the coating-substrate interface.
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