| 1. |
- Svensson, Lotta, 1959-
(author)
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Vinna och försvinna? : Drivkrafter bakom ungdomars utflyttning från mindre orter
- 2006
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Doctoral thesis (other academic/artistic)abstract
- Starting in the everyday life of Söderhamn’s youth, this thesis presents the driving forces that affect youth as they make decisions to stay in or to leave their home region.The research has been done with an interactive approach in close collaboration with youth and adults, and a number of different methods have been used. Based on the interviews, essays, questionnaires, conversations and meetings that underlie this work, the ambition has been to participate and contribute to the formation of knowledge in dialog with the youth and the adults who surround them.The dissertation (thesis) shows that society’s basic structures have great influence on youth’s decision whether to stay or leave. Important factors are actual circumstances, such as the access that youth have to resources – economical, social, and cultural. Society’s gender order has a big impact on the question whether to move or stay. Likewise, the tension between center and periphery plays an essential role – where the power to define what is “right” and “natural” coincides with values on how youth “are” and “should be”.The dissertation (thesis) shows that the interplay between these factors appears different for different individuals and groups, and that the interplay has significance both for the move/stay –decision and for the youth’s desire and possibility to participate in and have influence over society’s development in the region.
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| 2. |
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| 3. |
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| 4. |
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| 5. |
- Andersson, Marica, et al.
(author)
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Increased noise levels cause behavioural and distributional changes in Atlantic cod and saithe in a large public aquarium—A case study
- 2023
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In: Aquaculture, Fish and Fisheries. - : John Wiley & Sons. - 2693-8847.
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Journal article (peer-reviewed)abstract
- Abstract Investigating the effects of underwater noise on aquatic animals is a research field that is receiving rapidly increasing attention. Despite this, surprisingly few studies have addressed the potential impacts of noise in a marine animal husbandry setting. In this regard, the behaviour of fish in public aquariums can be used as an indicator of well-being, and noise is known to cause behavioural changes. This case study investigates the behaviour of cod (Gadus morhua) and saithe (Pollachius virens) in a large public aquarium when exposed to increased noise levels originating from an aquarium renovation carried out by construction divers. Swimming behaviour, group formation and vertical distribution, along with yawning and scratching frequencies of the fish, were analysed from video recordings made before, during and after the exposure to increased noise levels. The same parameters were also analysed to evaluate potential effects of the presence of divers when not making renovation noise, compared to fish behaviour prior to the renovation. There was a slight change in the depth distribution of both species and a decrease in the number of scratches in cod due to the presence of divers that were not making renovation noise. In the presence of construction noises in the tank, however, both cod and saithe showed a wider array of behavioural changes, including increased swimming speed, changes in depth distribution and increased yawning frequencies. The results from this case study demonstrate that an underwater renovation with increased noise levels impacts fish behaviour and suggests that underwater noise should be considered during the management of aquatic environments, including public aquaria.
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| 6. |
- Arborelius, Lotta, et al.
(author)
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A new interactive computer simulation system for violence risk assessment of mentally disordered violent offenders
- 2013
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In: CBMH. Criminal behaviour and mental health. - : Wiley. - 0957-9664 .- 1471-2857. ; 23:1, s. 30-40
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Journal article (peer-reviewed)abstract
- Background Assessment of risk of future violence has developed from reliance on static indicators towards a more dynamic approach. In the latter context, however, the offender is seldom confronted with real life situations. Aims The aim of this study is to evaluate a computer-based system Reactions on Display, which presents human interactions based on real-life situations for its effectiveness in distinguishing between potentially violent offenders with mental disorder and a healthy comparison group. Methods Male offenders with autism spectrum disorders or psychosis were recruited from specialist forensic psychiatric units in Sweden and healthy participants from the local communities. Each consenting participant was presented with film clips of a man in neutral and violent situations, which at critical moments stopped the story to ask him to predict the thoughts, feelings and actions of the actor. Results Offender patients, irrespective of diagnosis, detected fewer emotional reactions in the actor in the non-violent sequence compared with controls. When asked to choose one of four violent actions, the offender patients chose more violent actions than did the controls. They also reported fewer physical reactions in the actors when actors were being violent. There were also some examples of incongruent or deviant responses by some individual patients. Conclusions and implications for practice The use of interactive computer simulation techniques is not only generally acceptable to offender patients, but it also helps to differentiate their current response style to particular circumstances from that of healthy controls in a way that does not rely on their verbal abilities and may tap more effectively into their emotional reactions than standard verbal questions and answer approaches. This may pave the way for Reactions on Display providing a useful complement to traditional risk assessment, and a training route with respect to learning more empathic responding, thus having a role in aiding risk management.
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| 7. |
- Armstrong, Andrea, et al.
(author)
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Lysosomal Network Proteins as Potential Novel CSF Biomarkers for Alzheimers Disease
- 2014
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In: Neuromolecular medicine. - : Humana Press. - 1535-1084 .- 1559-1174. ; 16:1, s. 150-160
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Journal article (peer-reviewed)abstract
- The success of future intervention strategies for Alzheimers disease (AD) will likely rely on the development of treatments starting early in the disease course, before irreversible brain damage occurs. The pre-symptomatic stage of AD occurs at least one decade before the clinical onset, highlighting the need for validated biomarkers that reflect this early period. Reliable biomarkers for AD are also needed in research and clinics for diagnosis, patient stratification, clinical trials, monitoring of disease progression and the development of new treatments. Changes in the lysosomal network, i.e., the endosomal, lysosomal and autophagy systems, are among the first alterations observed in an AD brain. In this study, we performed a targeted search for lysosomal network proteins in human cerebrospinal fluid (CSF). Thirty-four proteins were investigated, and six of them, early endosomal antigen 1 (EEA1), lysosomal-associated membrane proteins 1 and 2 (LAMP-1, LAMP-2), microtubule-associated protein 1 light chain 3 (LC3), Rab3 and Rab7, were significantly increased in the CSF from AD patients compared with neurological controls. These results were confirmed in a validation cohort of CSF samples, and patients with no neurochemical evidence of AD, apart from increased total-tau, were found to have EEA1 levels corresponding to the increased total-tau levels. These findings indicate that increased levels of LAMP-1, LAMP-2, LC3, Rab3 and Rab7 in the CSF might be specific for AD, and increased EEA1 levels may be a sign of general neurodegeneration. These six lysosomal network proteins are potential AD biomarkers and may be used to investigate lysosomal involvement in AD pathogenesis.
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| 8. |
- Baker, Gretchen, et al.
(author)
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Kinematics and Shoulder Belt Engagement of Children on Belt-Positioning Boosters during Emergency Braking Events
- 2017
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In: Conference proceedings International Research Council on the Biomechanics of Injury, IRCOBI. - 2235-3151.
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Conference paper (peer-reviewed)abstract
- Emergency braking can influence children’s posture and seatbelt interaction. To better protect children in crashes preceded by emergency braking, this study a ims to quantify kinematics and seatbelt engagement during braking events . Eighteen rear‐seated children were exposed to braking events with 1 g deceleration in a passenger vehi cle, restrained by the seatbelt on a booster cushion and an integrated booster cushion. Vehicle acceleration and video data were analysed to m easure head displacement and shoulder belt position. On the booster cushion the belt was generally mid‐sho ulder and lower on the torso with a gap, while on the integrated booster cushion i t was closer to the neck and hi gher on the torso without a gap. Average forward head displacement was 160 mm on the booster cushion and 150 mm on the integrated booster cushion. Generally, the belt maintained the same position on the shoulde r throughout braking, with exceptions influenced by shifted initial positions or non‐standard motions. Braking e vents placed the head approximately 150‐190 mm forward from the initial position, influenced by booster, statu re, and initial seatbelt positioning. This reinforces the importance of maintaining mid‐shoulder or close to neck bel t positions and upright, centred postures prior to emergency braking, which may influence the likelihood of imp acting the vehicle interior and sustaining head injuries in a subsequent crash.
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| 9. |
- Baker, Gretchen, 1994, et al.
(author)
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KINEMATICS AND SHOULDER BELT ENGAGEMENT OF CHILDREN ON BELT-POSITIONING BOOSTERS DURING EVASIVE STEERING MANEUVERS
- 2017
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Conference paper (other academic/artistic)abstract
- Objective: To increase the protection of child passengers in crashes preceded by evasive steering, understanding of how children interact with the seatbelt in such situations is essential. This study aims to quantify child kinematics and describe child-to-restraint interaction during evasive steering maneuvers. Methods: Eighteen child volunteers (aged 5-10) were seated on the rear seat of a passenger vehicle. A professional driver made repeatable sharp turns at 50 km/h. Children were restrained by the seatbelt on a booster cushion (BC) and on an integrated booster cushion (IBC). Kinematics of the nasion and upper sternum were analyzed with video tracking software and shoulder belt (SB) engagement and position were evaluated. Results: Children moved laterally inboard, and SB-to-body interaction was influenced by booster and stature. For shorter children, the SB was closer to the neck with more of the belt webbing in contact with the torso and a more curved belt paths on the IBC compared to the BC, where less of the SB was in contact with the torso and straight belt paths were observed throughout steering. Taller children generally had the SB initially mid-shoulder with more contact between the SB and torso, resulting in curved belt paths at initial and maximum displacement on both boosters. Children loaded the shoulder belt by axially rotating their torso into the SB more often on the IBC compared to BC. The SB generally stayed on the shoulder, with 89% of slip-off instances occurring for shorter children on the BC. Shorter children on the BC had the largest average inboard nasion displacement of 105 mm initially and 120 mm overall. Taller children on the BC had the lowest average inboard displacement of the nasion (85 mm initially, 100 mm overall). All children initially displaced on average between 65-75 mm inboard with their sternum and 90 mm overall. Conclusions: Initial SB position on the shoulder and torso differed with booster and stature, which influenced how children engaged with the seatbelt during steering. Children with less SB initially in contact with the torso moved laterally behind the belt, resulting in straighter SB paths and outboard motion of the SB on the shoulder (often ending far out or slipped-off). When more of the SB was initially in contact with the torso, children tended to engage the SB more, moving with the belt and causing the SB path to become more curved, resulting in less inboard head displacement and less outboard motion of the SB on the shoulder. Enhanced understanding of how evasive steering affects the kinematic response of children provides valuable data for protection of children in real world situations.
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| 10. |
- Baker, Gretchen, 1994, et al.
(author)
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Kinematics and shoulder belt engagement of children on belt-positioning boosters during evasive steering maneuvers
- 2018
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In: Traffic Injury Prevention. - : Informa UK Limited. - 1538-957X .- 1538-9588. ; 19:S1, s. S131-S138
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Journal article (peer-reviewed)abstract
- Objective: To increase the protection of child passengers in crashes preceded by evasive steering, understanding of how children interact with the seat belt in such situations is essential. This study aims to quantify child kinematics and describe child-to-restraint interaction during evasive steering maneuvers. Methods: Eighteen child volunteers (aged 5–10) were seated on the rear seat of a passenger vehicle. A professional driver made repeatable sharp turns at 50 km/h. Children were restrained by the seat belt on a booster cushion (BC) and on an integrated booster cushion (IBC). Kinematics of the nasion and upper sternum were analyzed with video tracking software and shoulder belt (SB) engagement and position were evaluated. Results: Children moved laterally inboard, and SB-to-body interaction was influenced by booster and stature. Shorter children displayed initial SB positions closer to the neck with less instances of gap between the SB and the lower torso, resulting in more curved belt paths on the IBC. On the BC, shorter children had less of the SB in contact with the torso and straight belt paths were observed throughout steering. Taller children generally had the SB initially mid-shoulder with less instances of gap, resulting in curved belt paths at initial and maximum displacements on both boosters. Children loaded the shoulder belt by axially rotating their torso into the SB more often on the IBC compared to BC. The SB generally stayed on the shoulder, with 89% of slip-off instances occurring for shorter children on the BC. Shorter children on the BC had the largest average inboard nasion displacement (120 mm). Taller children on the BC had the lowest average inboard displacement of the nasion (100 mm). All children initially displaced on average 90 mm inboard with their upper sternum. Conclusions: Initial SB position on the shoulder and torso differed with booster and stature, which influenced how children engaged with the seat belt during steering. Children with less SB initially in contact with the torso moved laterally behind the belt, resulting in straighter SB paths and outboard motion of the SB on the shoulder (often ending far out or slipped off). When more of the SB was initially in contact with the torso, children tended to engage the SB more, moving with the belt and causing the SB path to become more curved, resulting in less inboard head displacement and less outboard motion of the SB on the shoulder. Enhanced understanding of how evasive steering affects the kinematic response of children provides valuable data for protection of children in real-world situations.
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| 11. |
- Blom, Eva-Lotta, 1973, et al.
(author)
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Acoustic and visual courtship traits in two sympatric marine Gobiidae species – Pomatoschistus microps and Pomatoschistus minutus
- 2016
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In: Environmental Biology of Fishes. - : Springer Science and Business Media LLC. - 0378-1909 .- 1573-5133. ; 99, s. 999-1007
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Journal article (peer-reviewed)abstract
- Divergence in courtship traits across species can evolve as adaptations to different environments, and also through avoidance of reproductive interference and character displacement. Differences may also be explained by phylogenetic relationships. We compared different courtship traits, including male courtship sounds, in two sympatric Pomatoschistus species. Both species are characterised by having male and female courtship, and paternal care of eggs in nests under mussel shells and rocks. In addition to presenting novel observations, we reviewed the literature on courtship traits for both species and complemented it with new observations. We found that courting males of the common goby P. microps sing louder and produce sounds of shorter duration than males of the sand goby P. minutus. Furthermore, males of P. microps swim faster towards females during courtship than males of P. minutus. The eyes of P. minutus females turn black during courtship attempts, whereas this is not the case for females of P. microps. Species-specific differences in courtship sounds and behavior may lead to different susceptibility of the two species to environmental change such as noise pollution and turbidity.
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| 12. |
- Blom, Eva-Lotta, et al.
(author)
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Anthropogenic noise disrupts early-life development in a fish with paternal care
- 2024
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In: Science of the Total Environment. - 0048-9697 .- 1879-1026. ; 935
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Journal article (peer-reviewed)abstract
- Anthropogenic noise is a global pollutant but its potential impacts on early life-stages in fishes are largely unknown. Here, using controlled laboratory experiments, we tested for impacts of continuous or intermittent exposure to low-frequency broadband noise on early life-stages of the common goby (Pomatoschistus microps), a marine fish with exclusive paternal care. Neither continuous nor intermittent noise exposure had an effect on filial cannibalism, showing that males were capable and willing to care for their broods. However, broods reared in continuous noise covered a smaller area and contained fewer eggs than control broods. Moreover, although developmental rate was the same in all treatments, larvae reared by males in continuous noise had, on average, a smaller yolk sac at hatching than those reared in the intermittent noise and control treatments, while larvae body length did not differ. Thus, it appears that the increased consumption of the yolk sac reserve was not utilised for increased growth. This suggests that exposure to noise in early life-stages affects fitness-related traits of surviving offspring, given the crucial importance of the yolk sac reserve during the early life of pelagic larvae. More broadly, our findings highlight the wide-ranging impacts of anthropogenic noise on aquatic wildlife living in an increasingly noisy world.
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| 13. |
- Blom, Eva-Lotta, et al.
(author)
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Continuous and intermittent noise has a negative impact on reproductive success and early life survival in marine fish
- 2022
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Conference paper (peer-reviewed)abstract
- Anthropogenic underwater noise is a global pollutant of increasing concern and its effect on marine organisms is largely unknown. Importantly, direct assessments of fitness consequences are lacking especially in fish. The effect of noise pattern with continuous or intermittent noise are poorly understood and the few existing studies investigating the effect highlight contradictory responses in fish. Working in aquaria, we experimentally tested the impact of broadband noise exposure (similar frequency range as anthropogenic boat noise; added either continuously or intermittently) on the behaviour and reproductive success, assessed by the number of obtained eggs, of the common goby (Pomatoschistus microps), a vocal fish with exclusive paternal care. The continuous noise treatment had the most detrimental effect by reducing spawning probability and females took longer to spawn under continuous noise. Males exposed to continuous noise got significantly fewer egg clutches (4 compared to 11 and 15 in the intermittent noise and silence treatments). Clutch area did not differ among treatments but clutches in the intermittent and continuous noise treatment had significantly more eggs per cm2. In addition, eggs in the control tanks hatched earlier than in the intermittent and noisy treatments. Larvae reared in continuous noise treatment were larger and had a smaller yolk-sac at hatching than larvae in the intermittent noise treatment and the control. Taken together, we show that noise, particularly a continuous noise exposure, negatively affects reproductive success and early life survival in fish larvae.
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| 14. |
- Blom, Eva-Lotta, 1973, et al.
(author)
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Continuous but not intermittent noise has a negative impact on mating success in a marine fish with paternal care
- 2019
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In: Scientific Reports. - : Springer Science and Business Media LLC. - 2045-2322. ; 9
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Journal article (peer-reviewed)abstract
- Anthropogenic underwater noise is a global pollutant of increasing concern but its impact on reproduction in fish is largely unknown. Hence, a better understanding of its consequences for this important link to fitness is crucial. Working in aquaria, we experimentally tested the impact of broadband noise exposure (added either continuously or intermittently), compared to a control, on the behaviour and reproductive success of the common goby (Pomatoschistus microps), a vocal fish with exclusive paternal care. Compared to the intermittent noise and control treatments, the continuous noise treatment increased latency to female nest inspection and spawning and decreased spawning probability. In contrast, many other female and male pre-spawning behaviours, and female ventilation rate (proxies for stress levels) did not differ among treatments. Therefore, it is likely that female spawning decisions were delayed by a reduced ability to assess male acoustic signals, rather than due to stress per se and that the silent periods in the intermittent noise treatment provided a respite where the females could assess the males. Taken together, we show that noise (of similar frequency range as anthropogenic boat noise) negatively affects reproductive success, particularly under a continuous noise exposure.
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| 15. |
- Blom, Eva-Lotta, 1973, et al.
(author)
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Male acoustic display in the sand goby – Essential cue in female choice, but unaffected by supplemental feeding
- 2022
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In: Journal of Experimental Marine Biology and Ecology. - : Elsevier. - 0022-0981 .- 1879-1697. ; 556
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Journal article (peer-reviewed)abstract
- Many teleost fishes use acoustic and visual signalling during courtship. Such displays may convey information about body condition. Here we experimentally altered body condition of sand goby (Pomatoschistus minutus) males to examine effects on acoustic and visual courtship and subsequent spawning decisions. Over two weeks, males fed in excess were fed daily, whereas food-deprived males were fed once a week. Females only spawned with males that produced courtship sound. However, there were no treatment effects on the occurrence of spawning and males fed in excess did not invest more in visual or acoustic courtship than food-deprived males. That said, males fed in excess built more well-covered nests, with more sand piled on top, compared to food-deprived males. Male condition measured as lipid content differed significantly between treatments. However, only males fed in excess differed in lipid content from wild caught males, indicating that in nature, males are of similar condition to males in the low condition treatment group. Apart from the importance of courtship sound, the only male or female behaviour predicting reproductive success was if male displayed in the nest opening. Males often produce courtship sounds together with a visual display in this position. A female dark-eye display did not associate with reproductive success which, together with previous results, suggest a non-ornamental function of this trait. We conclude that male courtship sounds appear to be crucial in female mate choice, but the information content of the courtship sounds and how it relates to male condition remains elusive.
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| 16. |
- Boström, Ola, 1963, et al.
(author)
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Comparison of car seats in low speed rear-end impacts using the BioRID dummy and the new neck injury criterion (NIC).
- 2000
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In: Accident Analysis and Prevention. ; 32:2, s. 321-328
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Journal article (peer-reviewed)abstract
- Long-term whiplash associated disorders (WAD) 1-3 sustained in low velocity rear-end impacts is the most common disability injury in Sweden. Therefore, to determine neck injury mechanisms and develop methods to measure neck-injury related parameters are of importance for current crash-safety research. A new neck injury criterion (NIC) has previously been proposed and evaluated by means of dummy, human and mathematical rear-impact simulations. So far, the criterion appears to be sensitive to the major car and collision related risk factors for injuries with long-term consequences. To further evaluate the applicability of NIC, four seats were tested according to a recently proposed sled-test procedure. 'Good' as well as 'bad' seats were chosen on the basis of a recently presented disability risk ranking list. The dummy used in the current tests was the Biofidelic Rear Impact Dummy (BioRID). The results of this study showed that NICmax values were generally related to the real-world risk of long-term WAD 1-3. Furthermore, these results suggested that NICmax calculated from sled tests using the BioRID dummy can be used for evaluating the neck injury risk of different car seats.
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| 17. |
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| 18. |
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| 19. |
- Carlsson, Anna K, 1966, et al.
(author)
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Review of Child Car Occupant Fatalities in Sweden During Six Decades
- 2013
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In: Proceedings: IRCOBI Conference; September 11-13; Gothenburg, Sweden. ; , s. 1-14
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Conference paper (peer-reviewed)abstract
- The development of fatal outcome was reviewed based on crash data including all fatally injured 0–14 year old car occupants in Sweden during 1956–2011 and put in relation to general improvements in vehicle and road safety and implementation of restraint systems.The review revealed a substantial decrease in crash‐related fatalities among 0–14 year old car occupants during the past three decades, representing a significant drop of 83% compared to the highest scores in the 1960s–70s. During 1992–2011, a total of 194 crash‐related fatalities were registered; the majority occurred on high‐speed roads. Head injury was a primary cause of death, in a total of 54% of all cases. Two fifths of the crashes involved a single car, while three fifths involved other vehicles. In total, 24% of the children were unrestrained, and 59% of those were ejected during crashes. Among the restrained children, 56% were considered to be appropriately restrained according to Swedish recommendations. Crash severity, complex crash situation, fire and drowning were factors that contributed to the fatal outcome, even though the restraint usage was considered to be optimal.
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| 20. |
- Charlton, Judith, et al.
(author)
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Naturalistic Observation of Children in Cars: An International Partnership
- 2013
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Conference paper (other academic/artistic)abstract
- It is well known that in the rear seat of cars, small children squirm, slide, slump, sleep, play and interact with their fellow passengers. Our previous findings from a pilot study show that children rarely remain in an optimal position for the efficient functioning of their restraint systems throughout the duration of their journey. Such behaviours may not only affect restraint effectiveness but may also have a negative influence on driver performance and distraction. Moreover, quantification of children’s position and out-of-position (OOP) status (i.e., their actual position relative to the ideal position for which the technology was designed) has important implications for design of test programs using anthropomorphic test devices (ATD) intended to mimic the human occupant. For example, understanding true pre-crash positions may lead to different design specifications of rear seat restraint systems and energy management features of the vehicle interior compared with the kinds of solutions that might arise from evaluations with an in-position ATD. This paper builds on our preliminary research findings and describes the design of the first international large-scale study of children in cars which uses innovative methods to observe and quantify the positions of child occupants in cars and identify the injury effects of OOP status and its impact on driver distraction. The study will facilitate a paradigm-shifting advance in child occupant protection – from the concept of safety technology designed to protect an ideally positioned occupant to the concept of dynamic restraint systems that maintain optimal restraint over a range of expected child positions/movements in a vehicle. Outcomes of the research will directly inform the design of future restraints for children, the development of appropriate crash test procedures that account for natural positions of child occupants, and the development of community awareness messages to improve the safety of children.
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| 21. |
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| 22. |
- Fernell, Elisabeth, 1948, et al.
(author)
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Developmental profiles in preschool children with autism spectrum disorders referred for intervention.
- 2010
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In: Research in Developmental Disabilities. - : Elsevier BV. - 0891-4222 .- 1873-3379. ; 31:3, s. 790-799
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Journal article (peer-reviewed)abstract
- The aim was to characterize the panorama of developmental disorders in 208 preschool children with a clinical diagnosis of autism spectrum disorder (ASD), referred to a specialized centre, the Autism Centre for Young Children (ACYC), for intervention. At the centre, a research team examined all children according to structured protocols and interviews. All available test data from their assessments prior to referral were scrutinized. The boy:girl ratio was 5.5:1. In 22% of the total group a period of regression, including speech and language, had occurred. Epilepsy had been diagnosed in 6% of the children. In 38% of the children there was a definite or highly suspected learning disability/mental retardation according to cognitive test results. About the same proportion had a developmental delay that at the time of assessment could not be definitely classified and in 23% there were clear indications of a normal intellectual function. About 40% of the group exhibited hyperactivity. Differences in expressive vocabulary and adaptive functioning were strongly related to cognitive level. About 20% of the group had AD as the dominating developmental disorder, i.e., they represented a clinical picture of "classic" autism. The majority in this group also had learning disability. Another 20%, had ASD combined with a normal intellectual level, some of these conformed to the clinical picture of Asperger syndrome. In a relatively large group (more than half) learning disability or a general developmental delay was as evident as the ASD. In a smaller group (8%) ASD criteria were questionably met. In this group attention deficits in connection with speech and language problems were prominent. The highly individual developmental profiles seen in children with ASDs have to be taken into account when planning intervention and follow-up. The children's medical characteristics also vary considerably and will be detailed in a further report.
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| 23. |
- Gidekull, Maria, et al.
(author)
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alpha-Tocopherol and beta-carotene concentrations in feed, colostrum, cow and calf serum in Swedish dairy herds with high or low calf mortality
- 2018
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In: Acta Veterinaria Scandinavica. - : Springer Science and Business Media LLC. - 0044-605X .- 1751-0147. ; 60
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Journal article (peer-reviewed)abstract
- Background: A study of herd-level risk factors for calf mortality in large Swedish dairy herds showed low serum concentrations of alpha-tocopherol and beta-carotene in 1-7 day old calves to be more common in high mortality herds. Therefore, we aimed to investigate if calf mortality risk at herd level is associated with concentrations of alpha-tocopherol and/or beta-carotene at individual level in feed, colostrum, cow and calf serum, while controlling for herd level covariates. Inclusion criteria were affiliation to the Swedish official milk recording scheme, herd size of >= 120 milking cows/year, calf mortality risk (day 1-90) of at least 6% (high mortality; HM) or less than 1% (low mortality; LM) and located within one of two regions in southern Sweden. This cross-sectional study was performed in 2010 in 19 -(n(HM) = 9; -n(LM) = 10) dairy herds. Questionnaires were used to collect information about feed and routines for colostrum feeding. Feed (n = 57), colostrum (n = 162), cow serum (n = 189) and calf serum samples (n = 187) were collected and analysed for alpha-tocopherol and beta-carotene. Other analyses e.g. total serum protein, fat content, and total solids in colostrum were also performed. Linear regression models with vitamin concentrations in feed, colostrum, cow and calf serum as outcome were performed.Results: Calves in HM herds had lower concentrations of alpha-tocopherol in serum than calves in LM herds, but the effect depended on total protein status in serum of the calf (P = 0.036). Calves from herds that fed transition milk for 3 days or more had higher alpha-tocopherol concentrations in serum than calves from herds feeding transition milk up to 2 days (P = 0.013). Fat percentage in colostrum was positively associated with alpha-tocopherol (P < 0.001) and beta-carotene concentrations in colostrum (P < 0.001). A diet containing >= 20% (in kg dry matter) maize silage of the total ration was negatively associated with beta-carotene concentration in cow serum (P = 0.001).Conclusions: High calf mortality risks were associated with lower concentrations of alpha-tocopherol in calf serum for calves with failure of passive transfer. Feeding transition milk longer was associated with higher concentrations of alpha-tocopherol in calf serum. In HM herds, evaluation of the calves' alpha-tocopherol status is recommended.
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| 24. |
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| 25. |
- Happonen, Lotta, et al.
(author)
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A quantitative Streptococcus pyogenes-human protein-protein interaction map reveals localization of opsonizing antibodies
- 2019
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In: Nature Communications. - : Springer Science and Business Media LLC. - 2041-1723. ; 10:1, s. 2727-2727
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Journal article (peer-reviewed)abstract
- A fundamental challenge in medical microbiology is to characterize the dynamic protein-protein interaction networks formed at the host-pathogen interface. Here, we generate a quantitative interaction map between the significant human pathogen, Streptococcus pyogenes, and proteins from human saliva and plasma obtained via complementary affinity-purification and bacterial-surface centered enrichment strategies and quantitative mass spectrometry. Perturbation of the network using immunoglobulin protease cleavage, mixtures of different concentrations of saliva and plasma, and different S. pyogenes serotypes and their isogenic mutants, reveals how changing microenvironments alter the interconnectivity of the interaction map. The importance of host immunoglobulins for the interaction with human complement proteins is demonstrated and potential protective epitopes of importance for phagocytosis of S. pyogenes cells are localized. The interaction map confirms several previously described protein-protein interactions; however, it also reveals a multitude of additional interactions, with possible implications for host-pathogen interactions involving other bacterial species.
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| 26. |
- Jakobsson, Lotta, 1967, et al.
(author)
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ASSESSMENT OF PASSENGER SAFETY IN FUTURE CARS - IDENTIFYING THE REAL-WORLD NEEDS TOWARDS SAFETY SYSTEM DEVELOPMENT
- 2023
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In: 27th ESV Conference Proceedings.
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Conference paper (peer-reviewed)abstract
- Future cars will likely include further collision mitigation systems, seat positions and seating configurations compared to current cars, in addition to an increased degree of shared mobility solutions. At the same time the population is becoming older and the diversity in car passenger dimensions is growing. This calls for assessment tools and evaluation methods beyond the current standardized crash test methods. This paper summarizes the results of a Swedish research project on how to assess the protection of the heterogeneous population of passengers (i.e., nondrivers) in future car crashes, focusing on restraint interaction. With the overall purpose of further improving passenger protection, the specific aims were to achieve method developments based on the enhancement of tools (physical and virtual human substitutes) as well as to create knowledge on passenger protection needs. This comprehensive research project combined multiple competencies and international collaborations, and a large number of studies have been performed using different methods. The applied methods include real-world crash data analyses to identify scenarios and situations, crash testing and simulation, and additionally user-studies conducted in cars to evaluate sitting posture, beltfit, kinematics, comfort, experiences and attitudes. Furthermore, the project included studies on crash test dummies (ATDs) and Human Body Models (HBM). Moreover, adult morphed HBMs were developed in various sizes, ages and sexes, for investigating various protection principles. In novel studies, crash interventions strategies were applied to predicted residual crash configurations. User-studies provided evidence of self-selected passenger postures in real car settings and, thus, deviations from standardized ATD positions. The importance of body shape was highlighted in a beltfit user-study including older adults. Essential booster design parameters were identified for children in upright and reclined seat positions. Restraint principles were investigated for adults in reclined seat positions and with the seat in rearward positions, away from the frontal airbag and knee bolster, along with an evaluation of the capabilities of the assessment tools. The adult HBM morphed to various sizes, ages and sexes were validated for prediction of in-crash kinematics in different impact scenarios, and provided enhanced insights in passenger protection assessment compared to the three standardized sizes of ATDs. Simulations with PIPER6y, a child-sized HBM, emphasized the importance of vehiclebooster-user system interaction. The results from the research project provided input to safety system development, ATD/HBM design, assessment methods development, and a number of identified research challenges for future work. Specifically, there is a need to further explore car passenger interaction with the restraint system in terms of seat positions and variations in body sizes, shapes and postures. The inclusions of the heterogeneous population into more advanced tools such as HBMs are essential, acknowledging that when moving closer to “zero injuries”, the situations to address are more unique and specific. Although a large range of studies using different methods was conducted, many challenges still remain to cover the entire scope of passenger safety in future cars.
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| 27. |
- Jakobsson, Lotta, 1967, et al.
(author)
-
Evaluation criteria for AIS 1 neck injuries in frontal impacts - A parameter study combining field data and Madymo modelling
- 2004
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In: Traffic Injury Prevention. - : Informa UK Limited. - 1538-957X .- 1538-9588. ; 5:4, s. 374-381
-
Journal article (peer-reviewed)abstract
- Two situations with an expected higher AIS 1 neck injury rate in frontal impact were compared to a reference situation using a Madymo human body model in three different sitting postures and four different crash pulses. The two situations were reduced occupant weight and occupant with initial forward arm resistance, respectively. Occupant neck motion phases were identified and corresponding possible evaluation criteria were evaluated within the phases. Typical neck kinematics was seen for the two different situations. Occupants of lower weight had a more extended neck in the initial protraction phase and also a generally more pronounced upper neck link angle. Occupants with initial arm resistance had generally greater lower neck link angle at the time when the upper neck link angle was straight. No evaluation criteria reflected the anticipated AIS 1 neck injury rate consistently. In the initial protraction phase, NICmincorrelated to expected injury outcome in almost half of the cases. In the protractionflexion shift phase, Nkm, Nij, upper neck shear force and neck tension force reflected anticipated severity outcome to some extent. In the flexion phase, upper and lower neck extension correlated to anticipated AIS 1 neck injury rate only to a minor extent. The different sitting postures were more influential than the different crash pulses, emphasizing the importance of not only considering the spectra of impact severity but also differences in sitting postures in safety system development and evaluation.
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| 28. |
- Jakobsson, Lotta, 1967, et al.
(author)
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Parameters influencing AIS 1 neck injury outcome in frontal impacts
- 2004
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In: Traffic Injury Prevention. - : Informa UK Limited. - 1538-957X .- 1538-9588. ; 5:2, s. 156-63
-
Journal article (peer-reviewed)abstract
- In order to gain more knowledge of the neck injury scenario in frontal impacts, a statistical study of parameters influencing incidences of AIS 1 neck injuries was performed. The data set consisted of 616 occupants in Volvo cars. Information regarding the crash, the safety systems, occupant characteristics (including prior neck problems), behavior and sitting posture at the time of impact, and neck symptoms (including duration) was collected and analyzed. Occupant characteristics (mainly gender, weight, and age), kinematics (head impacts) and behavior at the time of impact were identified as the most prominent parameter areas with regard to AIS 1 neck injury outcome. Specifically, women had a significantly higher AIS 1 neck injury rate as compared to men, occupants under the age of 50 had a significantly higher AIS 1 neck injury rate as compared to those above 50 and occupants weighing less than 65 kg have a significantly higher AIS 1 neck injury rate than heavier occupants. Drivers stating that they impacted their head against a frontal interior structure had a significantly higher AIS 1 neck injury rate than those without head impact. Also, occupants who stated they had tensed their neck muscles at the time of impact, had a significantly higher AIS 1 neck injury rate as compared to occupants who did not. Occupant activities, such as tightly gripping the steering wheel or straightening their arms showed a significantly increased AIS 1 neck injury rate, indicating that occupant behavior at time of impact could be influential with respect to AIS 1 neck injury outcome. Also, occupants reporting prior neck problems had a higher rate of persistent symptoms (>1 year) but no difference with respect to passing symptoms (
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| 29. |
- Jakobsson, Lotta, 1967, et al.
(author)
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Rear Seat Safety for Children Aged 4-12; Identifying the Real-World Needs Towards Development of Countermeasures
- 2017
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In: Proceedings of the 25th International Technical Conference on the Enhanced Safety of Vehicles.
-
Conference paper (other academic/artistic)abstract
- This study summarizes a joint research project aiming to further enhance the safety of booster-seated children (aged 4-12) in the rear seat of passenger cars. The focus is real-world aspects of child safety, comprising the whole context of the vehicle and child restraints, and a variety of crash situations, including pre-crash events.Real children sit in a variety of sitting postures in cars. On-road driving studies show that children take different postures due to comfort, visibility or activities. The results from three studies 18 children in a variety of restraints, showed that for only a portion of the time, they are sitting upright with contact to the seatback, i.e. similar to the standardized crash test dummy position. When using a booster with protruding head side supports the children sit forward leaning more than without, and in a large share of the time, the head is in front of the head side supports.Approximately 40% of the crashes are preceded by evasive maneuvers. When exposing child volunteers to evasive braking they will move forward by up to 0.2m, when shoulder belt remains over the shoulder. Thirty four child volunteers were exposed to evasive braking and steering events, using different types of boosters. Depending of the size of the child and the booster used, they might slide out of the shoulder belt in steering events. In addition, existing child crash test dummies were tested and compared to the volunteer data. The volunteer data was also used to validate an active child Human Body Model, as a first step to develop a tool that can be used for evasive maneuvers.The booster is essential for the child enabling good interaction to the seatbelt. In addition, the vehicle protection systems play an important role for the child protection. Hence, for enhancing real-world safety it is essential to replicate in-vehicle situations. Unfortunately, this is not how child restraints are certified today. This study shows that child crash test dummies benefit from side airbags and advanced seatbelt technologies, and are responsive to changes in sitting postures and crash modes. In addition to the in-crash protective systems evaluated in this study, pioneering maneuver and run-off-road tests with crash test dummies were run to evaluate the effect of an electrical reversible seatbelt retractor (pre-pretensioner) to help keep the occupant in the belt during such an event.International multidisciplinary workshops were held and concluded that future advancements need to be data driven and incorporate multiple disciplines. Engineering advancements should strive towards less complex solutions and the shared responsibility between the child restraint and the vehicle was highlighted.The results from this project contribute to identification and quantification of important real-world needs, as well as evaluation and development of countermeasures. It is concluded, that from a real-world perspective, the vehicle and child restraint should be designed together targeting a range of acceptable common user positions; sitting postures preferably guided by comfort and positive means. Such designs will ensure robust function of the protection systems for these young occupants, and advance the development of countermeasures that protect children in real- world crashes, also including dynamic events prior to a crash.
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| 30. |
- Jakobsson, Lotta, 1967, et al.
(author)
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REAR SEAT SAFETY IN FRONTAL TO SIDE IMPACTS – FOCUSING ON OCCUPANTS FROM 3YRS TO SMALL ADULTS
- 2011
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In: The 22nd ESV Conference Proceedings.
-
Conference paper (peer-reviewed)abstract
- ABSTRACTThis study presents a broad comprehensiveresearch effort that combines expertise fromindustry and academia and uses variousmethodologies with applied research directedtowards countermeasures. The project includesreal world crash data analysis, real world drivingstudies and crash testing and simulations,aiming at enhancing the safety of forward facingchild occupants (aged 3y to small adults) in therear seat during frontal to side impacts.The real world crash data analyses of properlyrestrained children originate from European aswell as US data. Frontal and side impact crashtests are analyzed using different sizes of crashtest dummies in different sitting postures. Sideimpact parameter studies using FE-models arerun. The sitting posture and behavior of 12children are monitored while riding in the rearseat. Also, the body kinematics and belt positionduring actual braking and turning maneuvers arestudied for 16 rear seat child occupants and forvarious child dummies.Real world crash data indicates that several ofthe injured children in frontal impacts, despitebeing properly restrained, impacted the vehicleinterior structure with their head/face resulting inserious injury. This was attributed to obliquecrashes, pre-crash vehicle maneuvers or highcrash severity. Crash tests confirm theimportance of proper initial belt-fit for bestprotection. The crash tests also highlight thedifficulty in obtaining the real world kinematicsand head impact locations using existing crashtest dummies and test procedures. The sideimpact parameter studies indicate that thevehicle’s occupant protection systems, such asairbags and seat belt pretensioners, play animportant role in protecting children as well.The results from the on-road driving studiesillustrate the variation of sitting postures duringriding in the rear seat giving valuable input to theeffects of the restraint systems and to howrepresentative the standardized dummy seatingpositioning procedures are. The results from themaneuver driving studies illustrate theimportance of understanding the kinematics of achild relative to the seat belt in a real worldmaneuver situation.Real world safety of rear seat occupants,especially children, involves evaluation ofprotection beyond standard crash testingscenarios in frontal and side impact conditions.This project explores the complete context ofrear seat protection in impact situations rangingfrom front to side and directions in betweenhighlighting the importance of pre-crash postureand behavior.This research project at SAFER (Vehicle andTraffic Safety Centre at Chalmers), whereresearchers from the industry and universitiescooperate with the aim to further improve safetyfor children (from 3y) to small adults in the rearseat, speeds up the process to safetyimplementation due to the interaction betweenacademic and industrial researchers.
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| 31. |
- Johnsdotter, Sara
(creator_code:cre_t, creator_code:res_t)
-
Forskarnas galleri #2 : 6 om sex
- 2017
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Artistic workabstract
- Utgångspunkten är en presentation av sex forskare, knutna till Centrum för sexologi och sexualitetsstudier, som berättar om sina respektive forskningsfält. Utställningen består också av en tidslinje med nedslag i den svenska sexualitetshistorien, ett tittskåp med kuriosa och en samling litteratur. I en brevlåda kan besökaren kommentera eller ställa frågor till forskarna, svaren projiceras på väggen. Två offentliga samtal arrangeras och filmas; Sex och makt och Tema erotisk litteratur. I samband med utställningen visas konstprojektet "Kyss" där prästen och konstnären Kent Wisti och författaren Maria Küchen i bild och text tolkat Höga visan.
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| 32. |
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| 33. |
- Jägervi, Lotta, et al.
(author)
-
Conceptions of gender and age in Swedish victim support
- 2015
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In: International Review of Victimology. - : SAGE Publications. - 0269-7580 .- 2047-9433.
-
Journal article (peer-reviewed)abstract
- The aim of this article is to unpack how the categories ‘gender’ and ‘age’ are understood among Swedish Victim Support organizations. This is done through a study of how individuals who work in Swedish Victim Support organizations talk about victims as well as about their own role. In an analysis of 12 focus groups from Swedish Victim Support organizations, where one part was based on vignettes and the other part on open discussion, we focus on how the supporters understand gender and age. By working with three categorical pairs – victim and supporter, male and female as well as young and old – we show that gender is a dominant categorization in understanding both victims and victim supporters. Age seemed to have more importance in how women are understood, as female victims were described quite differently depending on age, and the ideal helper was described as a mature woman. Male helpers were regarded as a homogenous group and age did not matter. Conclusively, we found that, while gender as a category was important in the understanding of how people are perceived in Victim Support, the intersection of the categories of gender and age were important in supporting and enhancing this understanding. This article points at the importance of understanding how these specific categories work.
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| 34. |
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| 35. |
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| 36. |
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| 37. |
- Linder, Astrid, et al.
(author)
-
Design and validation of the neck for a rear impact dummy (BioRID I)
- 2002
-
In: Traffic Injury Prevention. - : Informa UK Limited. - 1538-9588 .- 1538-957X. ; 3:2, s. 167-174
-
Journal article (peer-reviewed)abstract
- To assess the protective performance of seats and head restraints, occupant models able to mimic the motion of a human in a crash are needed. Hence, a new mechanical dummy neck for low-velocity rear collision tests was developed. The dummy neck consists of seven cervical elements connected by pin joints. The stiffness properties of the neck were represented by rubber blocks mounted between each pair of vertebrae, as well as by muscle substitutes between the head and the first thoracic vertebra (T1). The muscle substitutes consist of cables connected to a unit containing springs and a damper. The neck was validated against volunteer test data (Δv of 7 km/h) and compared with the kinematics of the Hybrid III dummy. The new neck was tested as a part of a new dummy (BioRID) that produced a human-like motion of the T1. The kinematics of the new neck was within the corridor of the volunteers, during the major part of the first 250 ms of the crash event, for both displacement of the head relative to T1 and for the acceleration of the head. This applies to both duration and peak values. When compared with the new neck, the Hybrid III showed an earlier decrease of the horizontal acceleration of the head, less maximum horizontal displacement, and an earlier increase of the rearward angular displacement of the head relative to T1.
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| 38. |
- Linder, Astrid, et al.
(author)
-
The New Neck Design for the Rear-End Impact Dummy, BioRID I
- 1998
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In: Annual Proceedings. - : Association for the Advancement of Automotive Medicine. ; , s. 172-192
-
Conference paper (other academic/artistic)abstract
- A new mechanical neck was developed for a new dummy possessing a complete articulated spine, for low speed rear-end collisions. The new neck consists of seven cervical elements connected by hinge joints. The neck stiffness properties were created by rubber blocks between each pair of vertebrae in combination with simulated muscle elements between the head and T1. The neck was validated against volunteer tests (Δv of 7 km/h) results. Both displacement and acceleration of the head relative to the upper torso for both duration and peak values, were in agreement with the volunteer data.
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| 39. |
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| 40. |
- Lindgren, Cecilia, et al.
(author)
-
Broad-Spectrum Antidote Discovery by Untangling the Reactivation Mechanism of Nerve-Agent-Inhibited Acetylcholinesterase
- 2022
-
In: Chemistry - A European Journal. - : John Wiley & Sons. - 0947-6539 .- 1521-3765. ; 28:40
-
Journal article (peer-reviewed)abstract
- Reactivators are vital for the treatment of organophosphorus nerve agent (OPNA) intoxication but new alternatives are needed due to their limited clinical applicability. The toxicity of OPNAs stems from covalent inhibition of the essential enzyme acetylcholinesterase (AChE), which reactivators relieve via a chemical reaction with the inactivated enzyme. Here, we present new strategies and tools for developing reactivators. We discover suitable inhibitor scaffolds by using an activity-independent competition assay to study non-covalent interactions with OPNA-AChEs and transform these inhibitors into broad-spectrum reactivators. Moreover, we identify determinants of reactivation efficiency by analysing reactivation and pre-reactivation kinetics together with structural data. Our results show that new OPNA reactivators can be discovered rationally by exploiting detailed knowledge of the reactivation mechanism of OPNA-inhibited AChE.
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| 41. |
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| 42. |
- Lumbers, R. T., et al.
(author)
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The genomics of heart failure: design and rationale of the HERMES consortium
- 2021
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In: Esc Heart Failure. - : Wiley. - 2055-5822. ; 8:6, s. 5531-5541
-
Journal article (peer-reviewed)abstract
- Aims The HERMES (HEart failure Molecular Epidemiology for Therapeutic targets) consortium aims to identify the genomic and molecular basis of heart failure. Methods and results The consortium currently includes 51 studies from 11 countries, including 68 157 heart failure cases and 949 888 controls, with data on heart failure events and prognosis. All studies collected biological samples and performed genome-wide genotyping of common genetic variants. The enrolment of subjects into participating studies ranged from 1948 to the present day, and the median follow-up following heart failure diagnosis ranged from 2 to 116 months. Forty-nine of 51 individual studies enrolled participants of both sexes; in these studies, participants with heart failure were predominantly male (34-90%). The mean age at diagnosis or ascertainment across all studies ranged from 54 to 84 years. Based on the aggregate sample, we estimated 80% power to genetic variant associations with risk of heart failure with an odds ratio of >1.10 for common variants (allele frequency > 0.05) and >1.20 for low-frequency variants (allele frequency 0.01-0.05) at P < 5 x 10(-8) under an additive genetic model. Conclusions HERMES is a global collaboration aiming to (i) identify the genetic determinants of heart failure; (ii) generate insights into the causal pathways leading to heart failure and enable genetic approaches to target prioritization; and (iii) develop genomic tools for disease stratification and risk prediction.
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| 43. |
- Manderstedt, Eric, et al.
(author)
-
Classic Thrombophilias and Thrombotic Risk Among Middle-Aged and Older Adults : A Population-Based Cohort Study
- 2022
-
In: Journal of the American Heart Association. - : Wiley-Blackwell Publishing Ltd. - 2047-9980. ; 11:4
-
Journal article (peer-reviewed)abstract
- BACKGROUND: Five classic thrombophilias have been recognized: factor V Leiden (rs6025), the prothrombin G20210A variant (rs1799963), and protein C, protein S, and antithrombin deficiencies. This study aimed to determine the thrombotic risk of classic thrombophilias in a cohort of middle-aged and older adults. METHODS AND RESULTS: Factor V Leiden, prothrombin G20210A and protein-coding variants in the PROC (protein C), PROS1 (protein S), and SERPINC1 (antithrombin) anticoagulant genes were determined in 29 387 subjects (born 1923–1950, 60% women) who participated in the Malmö Diet and Cancer study (1991–1996). The Human Gene Mutation Database was used to define 68 disease-causing mutations. Patients were followed up from baseline until the first event of venous thromboembolism (VTE), death, or Dec 31, 2018. Carriership (n=908, 3.1%) for disease-causing mutations in the PROC, PROS1, and SERPINC1 genes was associated with incident VTE: Hazard ratio (HR) was 1.6 (95% CI, 1.3–1.9). Variants not in Human Gene Mutation Database were not linked to VTE (HR, 1.1; 95% CI, 0.8–1.5). Heterozygosity for rs6025 and rs1799963 was associated with incident VTE: HR, 1.8 (95% CI, 1.6–2.0) and HR, 1.6 (95% CI, 1.3–2.0), respectively. The HR for carrying 1 classical thrombophilia variant was 1.7 (95% CI, 1.6–1.9). HR was 3.9 (95% CI, 3.1–5.0) for carriers of ≥2 thrombophilia variants. CONCLUSIONS: The 5 classic thrombophilias are associated with a dose-graded risk of VTE in middle-aged and older adults. Disease-causing variants in the PROC, PROS1, and SERPINC1 genes were more common than the rs1799963 variant but the conferred genetic risk was comparable with the rs6025 and rs1799963 variants.
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| 44. |
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| 45. |
- Manderstedt, Eric, et al.
(author)
-
Thrombomodulin (THBD) gene variants and thrombotic risk in a population-based cohort study
- 2022
-
In: Journal of Thrombosis and Haemostasis. - : Elsevier BV. - 1538-7933 .- 1538-7836. ; 20:4, s. 929-935
-
Journal article (peer-reviewed)abstract
- Background: The protein C anticoagulant system plays a key role in maintaining the hemostatic balance. Although several studies have identified thrombomodulin gene (THBD) variants among venous thromboembolism (VTE) patients, the role of THBD in relation to VTE in humans remains to be clarified. Objectives: This study aimed to determine the thrombotic risk of rare and common THBD variants in a large population-based cohort of middle-aged and older adults. Patients/Methods: The exome sequence of THBD was analyzed for qualifying variants in 28,794 subjects (born 1923–1950, 60% women), who participated in the Malmö Diet and Cancer study (1991–1996). Patients were followed from baseline until the first event of VTE, death, or 2018. Qualifying variants were defined as loss-of-function or non-benign (PolyPhen-2) missense variants with minor allele frequency <0.1%. Results: The single common coding variant rs1042579 was not associated with incident VTE. Sixteen rare variants were classified as qualifying and included in collapsing analysis. Seven individuals with VTE compared to 24 individuals without VTE carried one qualifying variant. Cox multivariate regression analysis adjusted for age, sex, body mass index, systolic blood pressure, smoking and alcohol consumption, rs6025, rs1799963, and the top two eigenvectors from a principal components analysis showed a hazard ratio of 3.0 (95% confidence interval 1.4–6.3) for the rare qualifying variants. The distributions of qualifying variants in THBD showed a difference for individuals with and without incident VTE indicating a possible position effect. Conclusions: Rare qualifying THBD variants were associated with VTE, suggesting that rare variants in THBD contribute to development of VTE.
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| 46. |
- Manderstedt, Eric, et al.
(author)
-
Thrombotic Risk Determined by Protein C Receptor (PROCR) Variants among Middle-Aged and Older Adults : A Population-Based Cohort Study
- 2022
-
In: Thrombosis and Haemostasis. - : Georg Thieme Verlag KG. - 0340-6245 .- 2567-689X. ; 122:8, s. 1326-1332
-
Journal article (peer-reviewed)abstract
- Background The protein C (PC) anticoagulant system has a key role in maintaining hemostatic balance. One missense (Ser219Gly) variant in the PC receptor (PROCR) was associated with venous thromboembolism (VTE) in genome-wide association studies. Objectives This study aimed to determine the thrombotic risk of rare and common PROCR variants in a large population-based cohort of middle-aged and older adults. Methods The exonic sequence of PROCR was analyzed for the Ser219Gly variant and other qualifying variants in 28,794 subjects (born 1923-1950, 60% women) without previous VTE, who participated in the Malmö Diet and Cancer study (1991-1996). Incidence of VTE was followed up until 2018. Qualifying variants were defined as loss-of-function or nonbenign (PolyPhen-2) missense variants with minor allele frequencies (MAFs) <0.1%. Results Re-sequencing identified 36 PROCR variants in the study population (26,210 non-VTE exomes and 2,584 VTE exomes), 11 synonymous, 22 missense, and three loss-of-function variants. Kaplan-Meier analysis of the known Ser219Gly variant (rs867186) showed that homozygosity for this variant increased the risk of disease, whereas heterozygosity showed no effect. Cox multivariate regression analysis revealed an adjusted hazard ratio (HR) of 1.5 (95% confidence interval [CI]: 1.1-2.0). Fifteen rare variants were classified as qualifying and were included in collapsing analysis (burden test and SKAT-O). They did not contribute to risk. However, a Arg113Cys missense variant (rs146420040; MAF = 0.004) showed an increased VTE risk (HR = 1.3; 95% CI: 1.0-1.9). Conclusion Homozygosity for the Ser219Gly variant and a previously identified functional PROCR variant (Arg113Cys) was associated with VTE. Other variants did not contribute to VTE.
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| 47. |
- Manderstedt, Eric, et al.
(author)
-
Thrombotic risk determined by rare and common SERPINA1 variants in a population-based cohort study
- 2022
-
In: Journal of Thrombosis and Haemostasis. - : Elsevier BV. - 1538-7933 .- 1538-7836. ; 20:6
-
Journal article (peer-reviewed)abstract
- Background: Severe alpha-1-antitrypsin deficiency (AATD), phenotype PiZZ, was associated with venous thromboembolism (VTE) in a case-control study. Objectives: This study aimed to determine the genetic variation in the SERPINA1 gene and a possible thrombotic risk of these variants in a population-based cohort study. Patients/Methods: The coding sequence of SERPINA1 was analyzed for the Z (rs28929474), S (rs17580), and other qualifying variants in 28,794 subjects without previous VTE (born 1923–1950, 60% women), who participated in the Malmö Diet and Cancer study (1991–1996). Individuals were followed from baseline until the first event of VTE, death, or 2018. Results: Resequencing the coding sequence of SERPINA1 identified 84 variants in the total study population, 21 synonymous, 62 missense, and 1 loss-of-function variant. Kaplan-Meier analysis showed that homozygosity for the Z allele increased the risk of VTE whereas heterozygosity showed no effect. The S (rs17580) variant was not associated with VTE. Thirty-one rare variants were qualifying and included in collapsing analysis using the following selection criteria, loss of function, in frame deletion or non-benign (PolyPhen-2) missense variants with minor allele frequency (MAF)
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| 48. |
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| 49. |
- Mjösberg, Jenny, et al.
(author)
-
Systemic reduction of functionally suppressive CD4dimCD25highFoxp3+ Tregs in human second trimester pregnancy is induced by progesterone and 17θ-estradiol
- 2009
-
In: Journal of Immunology. - : The American Association of Immunologists. - 0022-1767 .- 1550-6606. ; 183:1, s. 759-769
-
Journal article (peer-reviewed)abstract
- CD4+CD25high regulatory T cells (Tregs) are implicated in the maintenance of murine pregnancy. However, reports regarding circulating Treg frequencies in human pregnancy are inconsistent, and the functionality and phenotype of these cells in pregnancy have not been clarified. The aim of this study was to determine the frequency, phenotype, and function of circulating Tregs in the second trimester of human pregnancy and the influence of progesterone and 17β-estradiol on Treg phenotype and frequency. Based on expressions of Foxp3, CD127, and HLA-DR as determined by multicolor flow cytometry, we defined a proper CD4dimCD25high Treg population and showed, in contrast to most previous reports, that this population was reduced in second trimester of pregnancy. Unexpectedly, Foxp3 expression was decreased in the Treg, as well as in the CD4+ population. These changes could be replicated in an in vitro system resembling the pregnancy hormonal milieu, where 17β-estradiol, and in particular progesterone, induced, in line with the pregnancy situation, a reduction of CD4dimCD25highFoxp3+ cells in PBMC from nonpregnant women. By coculturing FACS-sorted Tregs and autologous CD4+CD25– responder cells, we showed that Tregs from pregnant women still displayed the same suppressive capacity as nonpregnant women in terms of suppressing IL-2, TNF-, and IFN- secretion from responder cells while efficiently producing IL-4 and IL-10. Our findings support the view of hormones, particularly progesterone, as critical regulators of Tregs in pregnancy. Furthermore, we suggest that in the light of the results of this study, early data on circulating Treg frequencies in pregnancy need reevaluation.The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked advertisement in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
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| 50. |
- Moilanen, Johanna, et al.
(author)
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Being, becoming and belonging in constructing children’s lived citizenship with contact persons and contact families
- 2023
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In: Barn. - Trondheim : Norsk senter for barneforskning. - 0800-1669 .- 2535-5449. ; 41:2–3, s. 74-89
-
Journal article (peer-reviewed)abstract
- The article discusses children’s citizenship by linking the dimensions of being, becoming and belonging to the concept of lived citizenship in the context of contact person and contact family interventions. Drawing on empirical research from Finland and Sweden, the article elaborates on how to identify if, and if so, how, children’s lived citizenship is constructed in contact person and contact family interventions and to what extent these constructions leave room for children’s perspectives. The results of the study indicate that contact person and contact family interventions contribute to the construction of children’s citizenship in various ways that include the perspectives on children and childhood of being, becoming and belonging. However, children’s active participation often seems to be governed by adults. Thus, giving greater attention to children’s intergenerational connections, i.e., adults’ roles in forming children’s citizenship, is needed.
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