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- Klionsky, Daniel J., et al.
(författare)
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Guidelines for the use and interpretation of assays for monitoring autophagy
- 2012
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Ingår i: Autophagy. - : Informa UK Limited. - 1554-8635 .- 1554-8627. ; 8:4, s. 445-544
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Forskningsöversikt (refereegranskat)abstract
- In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process vs. those that measure flux through the autophagy pathway (i.e., the complete process); thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from stimuli that result in increased autophagic activity, defined as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (in most higher eukaryotes and some protists such as Dictyostelium) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the field understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field.
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- Galakhov, V. R., et al.
(författare)
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Electronic structure and x-ray spectra of defective oxides LixCoO2
- 2006
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Ingår i: Physical Review B. Condensed Matter and Materials Physics. - 1098-0121 .- 1550-235X. ; 74:4, s. 045120-
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Tidskriftsartikel (refereegranskat)abstract
- X-ray absorption, resonant x-ray emission, and x-ray photoelectron spectra of the valence band and core levels have been measured for LixCoO2 (0.6 <= x <= 1.0). Resonant O K alpha x-ray emission spectra of LiCoO2 showed localized excitonic states due to a dd transition between unoccupied and occupied Co 3d states. On the basis of measurements of Co 3s x-ray photoelectron and Co 2p and O 1s x-ray absorption spectra it was established that in defective cobaltites the electronic holes are localized mainly in O 2p states. Evidence of phase separation in LixCoO2 has been found.
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- Oberstedt, Andreas, et al.
(författare)
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Improved values for the characteristics of prompt-fission gamma-ray spectra from the reaction U-235(n(th), f)
- 2013
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Ingår i: Physical Review C. Nuclear Physics. - : American Physical Society. - 0556-2813 .- 1089-490X .- 2469-9985 .- 2469-9993. ; 87:5, s. 051602-
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Tidskriftsartikel (refereegranskat)abstract
- In this paper we present results from measurements of prompt gamma rays from the thermal neutron induced fission of U-235. Photons were measured in coincidence with fission fragments with cerium-doped LaCl3 and LaBr3 as well as CeBr3 scintillation detectors, which offer an intriguing combination of excellent timing resolution and good resolving power. The spectra measured with all employed detectors are in excellent agreement with respect to their shapes. Characteristic parameters were extracted for a gamma-energy range from 0.1 to 6.0 MeV and the results obtained with several detectors were averaged. From that, the average emission yield of prompt-fission gamma rays was determined to be (nu) over bar (gamma) = (8.19 +/- 0.11) per fission, the average energy per photon to be epsilon(gamma) = (0.85 +/- 0.02) MeV, and the total energy to be E-gamma,E-tot = (6.92 +/- 0.09) MeV. The uncertainties are much lower than the 7.5% requested for the modeling of advanced nuclear reactor cores. Estimating the influence of gamma rays with energies between 6 and 10 MeV on the values determined in this work revealed a negligible deviation of the order of the found uncertainties.
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- Oberstedt, Andreas, 1960-, et al.
(författare)
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New prompt fission gamma-ray spectral data and its implication on present evaluated nuclear data files
- 2013
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Ingår i: Physics Procedia. - : Elsevier BV. - 1875-3892 .- 1875-3884. ; 47, s. 156-165
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Tidskriftsartikel (refereegranskat)abstract
- In this paper we report on new spectral prompt gamma-ray measurements from the spontaneous fission of Cf-252 and thermal neutron-induced fission of U-236*. In both experiments, fission of U-236*. In both experiments, gamma-ray multiplicities, average and total gamma-energies were extracted. gamma-ray multiplicities, average and total.-energies were extracted. Apart from one recent measurement on Cf-252, about four decades have passed since the last dedicated experiments were reported in literature. Hence, there was a need for a revision, not only with respect to high priority nuclear data requests by the Nuclear Energy Agency (NEA). In the first mentioned experiment we have measured prompt fission gamma-rays with both cerium-doped LaBr3 and CeBr3 scintillation detectors, which both exhibit excellent timing and good energy resolution. The results from both detectors are in excellent agreement with each other and confirm the historical data. In the experiment on U-235(n(th), f) we employed cerium-doped LaCl3 detectors, together with the lanthanum bromide detectors mentioned above. Even here the first results indicate a good agreement with data from the early 1970' s. They are also in accordance with data in evaluated libraries like ENDF/B-VII.0, while this is not the case for Cf-252(SF). Hence, here an update is strongly recommended.
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- Számadó, S., et al.
(författare)
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The language of cooperation: reputation and honest signalling
- 2021
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Ingår i: Philosophical Transactions of the Royal Society of London. Biological Sciences. - : ROYAL SOC. - 0962-8436 .- 1471-2970. ; 376:1838
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Tidskriftsartikel (refereegranskat)abstract
- Large-scale non-kin cooperation is a unique ingredient of human success. This type of cooperation is challenging to explain in a world of self-interested individuals. There is overwhelming empirical evidence from different disciplines that reputation and gossip promote cooperation in humans in different contexts. Despite decades of research, important details of reputation systems are still unclear. Our goal with this theme issue is to promote an interdisciplinary approach that allows us to explore and understand the evolution and maintenance of reputation systems with a special emphasis on gossip and honest signalling. The theme issue is organized around four main questions: What are the necessary conditions for reputation-based systems? What is the content and context of reputation systems? How can reputations promote cooperation? And, what is the role of gossip in maintaining reputation systems and thus cooperation?
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- Wagner, L., et al.
(författare)
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Astrophysical S factor of the N 14 (p,γ) O 15 reaction at 0.4-1.3 MeV
- 2018
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Ingår i: Physical Review C. - : American Physical Society. - 2469-9985 .- 2469-9993. ; 97:1
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Tidskriftsartikel (refereegranskat)abstract
- The N14(p,γ)O15 reaction is the slowest reaction of the carbon-nitrogen cycle of hydrogen burning and thus determines its rate. The precise knowledge of its rate is required to correctly model hydrogen burning in asymptotic giant branch stars. In addition, it is a necessary ingredient for a possible solution of the solar abundance problem by using the solar N13 and O15 neutrino fluxes as probes of the carbon and nitrogen abundances in the solar core. After the downward revision of its cross section due to a much lower contribution by one particular transition, capture to the ground state in O15, the evaluated total uncertainty is still 8%, in part due to an unsatisfactory knowledge of the excitation function over a wide energy range. The present work reports precise S factor data at twelve energies between 0.357 and 1.292 MeV for the strongest transition, capture to the 6.79-MeV excited state in O15, and at ten energies between 0.479 and 1.202 MeV for the second strongest transition, capture to the ground state in O15. An R-matrix fit is performed to estimate the impact of the new data on astrophysical energies. The recently suggested slight enhancement of the 6.79-MeV transition at low energy could not be confirmed. The present extrapolated zero-energy S factors are S6.79(0)=1.24±0.11 keV b and SGS(0)=0.19±0.05 keV b.
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