| 1. |
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| 2. |
- Beal, Jacob, et al.
(författare)
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Robust estimation of bacterial cell count from optical density
- 2020
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Ingår i: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
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Tidskriftsartikel (refereegranskat)abstract
- Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
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| 3. |
- Arheimer, Berit, et al.
(författare)
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The IAHS Science for Solutions decade, with Hydrology Engaging Local People IN a Global world (HELPING)
- 2024
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Ingår i: Hydrological Sciences Journal. - 0262-6667 .- 2150-3435.
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Tidskriftsartikel (refereegranskat)abstract
- The new scientific decade (2023-2032) of the International Association of Hydrological Sciences (IAHS) aims at searching for sustainable solutions to undesired water conditions - may it be too little, too much or too polluted. Many of the current issues originate from global change, while solutions to problems must embrace local understanding and context. The decade will explore the current water crises by searching for actionable knowledge within three themes: global and local interactions, sustainable solutions and innovative cross-cutting methods. We capitalise on previous IAHS Scientific Decades shaping a trilogy; from Hydrological Predictions (PUB) to Change and Interdisciplinarity (Panta Rhei) to Solutions (HELPING). The vision is to solve fundamental water-related environmental and societal problems by engaging with other disciplines and local stakeholders. The decade endorses mutual learning and co-creation to progress towards UN sustainable development goals. Hence, HELPING is a vehicle for putting science in action, driven by scientists working on local hydrology in coordination with local, regional, and global processes.
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| 4. |
- Ding, Xue Bing, et al.
(författare)
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Impaired meningeal lymphatic drainage in patients with idiopathic Parkinson’s disease
- 2021
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Ingår i: Nature Medicine. - : Springer Science and Business Media LLC. - 1078-8956 .- 1546-170X. ; 27:3, s. 411-418
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Tidskriftsartikel (refereegranskat)abstract
- Animal studies implicate meningeal lymphatic dysfunction in the pathogenesis of neurodegenerative diseases such as Alzheimer’s disease and Parkinson’s disease (PD). However, there is no direct evidence in humans to support this role1–5. In this study, we used dynamic contrast-enhanced magnetic resonance imaging to assess meningeal lymphatic flow in cognitively normal controls and patients with idiopathic PD (iPD) or atypical Parkinsonian (AP) disorders. We found that patients with iPD exhibited significantly reduced flow through the meningeal lymphatic vessels (mLVs) along the superior sagittal sinus and sigmoid sinus, as well as a notable delay in deep cervical lymph node perfusion, compared to patients with AP. There was no significant difference in the size (cross-sectional area) of mLVs in patients with iPD or AP versus controls. In mice injected with α-synuclein (α-syn) preformed fibrils, we showed that the emergence of α-syn pathology was followed by delayed meningeal lymphatic drainage, loss of tight junctions among meningeal lymphatic endothelial cells and increased inflammation of the meninges. Finally, blocking flow through the mLVs in mice treated with α-syn preformed fibrils increased α-syn pathology and exacerbated motor and memory deficits. These results suggest that meningeal lymphatic drainage dysfunction aggravates α-syn pathology and contributes to the progression of PD.
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| 5. |
- Ma, Teng, et al.
(författare)
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Distributed Reconfigurable Intelligent Surfaces Assisted Indoor Positioning
- 2023
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Ingår i: IEEE Transactions on Wireless Communications. - : Institute of Electrical and Electronics Engineers (IEEE). - 1536-1276 .- 1558-2248. ; 22:1, s. 47-58
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Tidskriftsartikel (refereegranskat)abstract
- Recently, communications with the aid of reconfigurable intelligent surface (RIS), which operates with the aim of enhancing the system communication performance, have aroused extensive researches. Furthermore, the use of RIS for positioning has been considered. Therefore, we focus on a practical structure of indoor positioning assisted by distributed RISs through utilizing their ability to manipulate multipath signals, through the developed quasi-static and dynamic modes. Specifically, in the quasi-static mode, for reducing the implementation cost, the reflection coefficients for each RIS are preset and remain constant. In the dynamic mode, the reflection coefficients can be timely updated with a two-step positioning approach toward more accurate positioning performance. Furthermore, the Cramer-Rao lower bound of the developed positioning scheme is quantified through theoretical analysis. Both theoretical analysis and simulation results demonstrate that RIS has the potential to realize accurate positioning even with a single access point, due to its ability to mark the channel and replace traditional active positioning anchors. Meanwhile, we also show that the developed two-step positioning scheme can achieve considerable performance gain in accurate positioning.
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| 6. |
- Ma, Teng, et al.
(författare)
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Integrated Sensing and Communication for Wireless Extended Reality (XR) With Reconfigurable Intelligent Surface
- 2023
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Ingår i: IEEE Journal on Selected Topics in Signal Processing. - : Institute of Electrical and Electronics Engineers (IEEE). - 1932-4553 .- 1941-0484. ; 17:5, s. 980-994
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Tidskriftsartikel (refereegranskat)abstract
- Future wireless networks will witness ubiquitous human-machine interactions, where extended reality (XR) is expected to be a key scenario in next-generation mobile systems. In this article, we examine the integrated sensing and communication (ISAC) framework in XR, where a reconfigurable intelligent surface (RIS) may assist user (UE) positioning and communication. Specifically, a practical positioning algorithm based on multiple signal classification (MUSIC) with the aid of specially designed RIS configurations is conceived. Furthermore, we formulate the joint optimization of the UE beamformer and RIS phase shifter to maximize the channel capacity under Cramer-Rao lower bound (CRLB) constraints, which is solved by alternating optimization with gradient projection and manifold optimization. Finally, we use simulation results to demonstrate the feasibility of the conceived positioning algorithm and corroborate the effectiveness of the proposed optimization approach.
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| 7. |
- Ma, Teng, et al.
(författare)
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Spreading CDMA via RIS : Multipath Separation, Estimation, and Combination
- 2023
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Ingår i: IEEE Internet of Things Journal. - : Institute of Electrical and Electronics Engineers (IEEE). - 2327-4662. ; 10:13, s. 11396-11413
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Tidskriftsartikel (refereegranskat)abstract
- As a revolutionary technology for future wireless communications, reconfigurable intelligent surface (RIS), characterized by an efficient way of manipulating wireless signals, has been widely investigated in recent years toward enhancing signal quality, energy efficiency, throughput, and so on. However, in RIS-assisted Internet of Things (IoT), a new issue as multipath separation emerges, especially, when deploying multiple RISs to assist communication, since the devices may have limited signal processing capabilities. For alleviating this problem, we conceive a novel RIS-enabled code-division multiple access (CDMA) structure, where each RIS holds a specified time-varying coefficient to tag the channel. Moreover, multipath extraction is further considered, including a practical channel estimation approach along with theoretical derivations in terms of Cramér-Rao lower bound, mean-square error, as well as ergodic channel capacity. Simulation results corroborate the feasibility of the conceived RIS-CDMA structure and the effectiveness of the proposed multipath extraction approach.
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| 8. |
- McGinn, Steven, et al.
(författare)
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New Technologies for DNA analysis-A review of the READNA Project.
- 2016
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Ingår i: New Biotechnology. - : Elsevier BV. - 1876-4347 .- 1871-6784.
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Forskningsöversikt (refereegranskat)abstract
- The REvolutionary Approaches and Devices for Nucleic Acid analysis (READNA) project received funding from the European Commission for 4 1/2 years. The objectives of the project revolved around technological developments in nucleic acid analysis. The project partners have discovered, created and developed a huge body of insights into nucleic acid analysis, ranging from improvements and implementation of current technologies to the most promising sequencing technologies that constitute a 3(rd) and 4(th) generation of sequencing methods with nanopores and in situ sequencing, respectively.
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| 9. |
- Song, Jiao-Jiao, et al.
(författare)
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The 4f-Hybridization Strength in CemMnIn3m+2n Heavy-Fermion Compounds Studied by Angle-Resolved Photoemission Spectroscopy
- 2021
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Ingår i: Chinese Physics Letters. - : IOP Publishing. - 0256-307X .- 1741-3540. ; 38:10
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Tidskriftsartikel (refereegranskat)abstract
- We systemically investigate the nature of Ce 4f electrons in structurally layered heavy-fermion compounds CemMnIn3m+2n (with M = Co, Rh, Jr, and Pt, m = 1, 2, n = 0-2), at low temperature using on-resonance angle-resolved photoemission spectroscopy. Three heavy quasiparticle bands f(0), f(7/2)(1) and f(5/2)(1), are observed in all compounds, whereas their intensities and energy locations vary greatly with materials. The strong f(0) states imply that the localized electron behavior dominates the Ce 4f states. The Ce 4f electrons are partially hybridized with the conduction electrons, making them have the dual nature of localization and itinerancy. Our quantitative comparison reveals that the f(5/2)(1)-f (0) intensity ratio is more suitable to reflect the 4f-state hybridization strength.
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| 10. |
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| 11. |
- Wang, Y. J., et al.
(författare)
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Cyclic AMP in oocytes controls meiotic prophase I and primordial folliculogenesis in the perinatal mouse ovary
- 2015
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Ingår i: Development. - : The Company of Biologists. - 0950-1991 .- 1477-9129. ; 142:2, s. 343-351
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Tidskriftsartikel (refereegranskat)abstract
- In mammalian ovaries, a fixed population of primordial follicles forms during the perinatal stage and the oocytes contained within are arrested at the dictyate stage of meiotic prophase I. In the current study, we provide evidence that the level of cyclic AMP ( cAMP) in oocytes regulates oocyte meiotic prophase I and primordial folliculogenesis in the perinatal mouse ovary. Our results show that the early meiotic development of oocytes is closely correlated with increased levels of intra-oocyte cAMP. Inhibiting cAMP synthesis in fetal ovaries delayed oocyte meiotic progression and inhibited the disassembly and degradation of synaptonemal complex protein 1. In addition, inhibiting cAMP synthesis in in vitro cultured fetal ovaries prevented primordial follicle formation. Finally, using an in situ oocyte chromosome analysis approach, we found that the dictyate arrest of oocytes is essential for primordial follicle formation under physiological conditions. Taken together, these results suggest a role for cAMP in early meiotic development and primordial follicle formation in the mouse ovary.
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| 12. |
- Yuan, Ya Hua, et al.
(författare)
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Angle-resolved photoemission spectroscopy view on the nature of Ce 4f electrons in the antiferromagnetic Kondo lattice CePd5Al2
- 2021
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Ingår i: Physical Review B. - : American Physical Society (APS). - 2469-9969 .- 2469-9950. ; 103:12
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Tidskriftsartikel (refereegranskat)abstract
- We report an angle-resolved photoemission spectroscopy study of the antiferromagnetic Kondo lattice CePd5Al2, focusing on the quasi-two-dimensional k-space nature of its Fermi surface and, tuning photon energy to the Ce 4d-4f on-resonance transition, the hybridization of the Ce 4f state. A strong shoulder feature on the f0 peak was detected, suggesting hybridization between conduction and f bands. On-resonance spectra revealed narrow, yet hybridized quasiparticle bands with sharp peaks and ∼ 9 meV energy dispersion near the Fermi energy EF. The observed dispersive hybridized f band can be well described by a hybridization-band picture based on the periodic Anderson model (PAM). Hence, the 4f electrons in CePd5Al2 display a dual nature, with both localized and itinerant features, but with dominantly localized character.
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| 13. |
- Zhang, Xia, 1980, et al.
(författare)
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Flip chip assembly using carbon nanotube bumps and anisotropic conductive adhesive film
- 2010
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Ingår i: ECS Transactions. - : The Electrochemical Society. - 1938-5862 .- 1938-6737. - 9781607682639 ; 27:1, s. 825-830
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Konferensbidrag (refereegranskat)abstract
- Carbon Nanotube (CNT) has been proposed as a promising candidate material to build next generation flip chip interconnects in electronics. In this paper, flip chip assembly using CNT bumps and ACF is performed. To realize CNT-based interconnects, post-growth processes including deposition of metallic layers on CNT bundles and low temperature transfer of CNT bundles have been developed to overcome the problems of the high growth temperature of CNT as well as the poor adhesion between CNT bumps and substrates. Moreover, ACF has been used to assemble the CNT bumps on another Si substrate. The electrical test is performed also to verify the good contact between CNT bumps and ACF.
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| 14. |
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| 15. |
- Aad, G., et al.
(författare)
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- 2013
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Tidskriftsartikel (refereegranskat)
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| 16. |
- Aad, G., et al.
(författare)
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- 2013
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Tidskriftsartikel (refereegranskat)
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| 17. |
- Aad, G, et al.
(författare)
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- 2015
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swepub:Mat__t
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| 18. |
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- 2019
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Tidskriftsartikel (refereegranskat)
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