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Träfflista för sökning "WFRF:(To Yiu Ying Janet) "

Search: WFRF:(To Yiu Ying Janet)

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  • Duong-Thi, Minh-Dao, et al. (author)
  • Lipodisks integrated with weak affinity chromatography enable fragment screening of integral membrane proteins
  • 2016
  • In: The Analyst. - : Royal Society of Chemistry (RSC). - 0003-2654 .- 1364-5528. ; 141:3, s. 981-988
  • Journal article (peer-reviewed)abstract
    • Membrane proteins constitute the largest class of drug targets but they present many challenges in drug discovery. Importantly, the discovery of potential drug candidates is hampered by the limited availability of efficient methods for screening drug-protein interactions. In this work we present a novel strategy for rapid identification of molecules capable of binding to a selected membrane protein. An integral membrane protein (human aquaporin-1) was incorporated into planar lipid bilayer disks (lipodisks), which were subsequently covalently coupled to porous derivatized silica and packed into HPLC columns. The obtained affinity columns were used in a typical protocol for fragment screening by weak affinity chromatography (WAC), in which one hit was identified out of a 200 compound collection. The lipodisk-based strategy, which ensures a stable and native-like lipid environment for the protein, is expected to work also with other membrane proteins and screening procedures.
  •  
2.
  • Duong-Thi, Minh-Dao, et al. (author)
  • Lipodisks integrated with weak affinity chromatography enable fragment screening of integral membrane proteins
  • 2016
  • In: The Analyst. - 0003-2654 .- 1364-5528. ; 141:3, s. 981-988
  • Journal article (peer-reviewed)abstract
    • Membrane proteins constitute the largest class of drug targets but they present many challenges in drug discovery. Importantly, the discovery of potential drug candidates is hampered by the limited availability of efficient methods for screening drug-protein interactions. In this work we present a novel strategy for rapid identification of molecules capable of binding to a selected membrane protein. An integral membrane protein (human aquaporin-1) was incorporated into planar lipid bilayer disks (lipodisks), which were subsequently covalently coupled to porous derivatized silica and packed into HPLC columns. The obtained affinity columns were used in a typical protocol for fragment screening by weak affinity chromatography (WAC), in which one hit was identified out of a 200 compound collection. The lipodisk-based strategy, which ensures a stable and native-like lipid environment for the protein, is expected to work also with other membrane proteins and screening procedures.
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  • Result 1-2 of 2
Type of publication
journal article (2)
Type of content
peer-reviewed (2)
Author/Editor
Edwards, Katarina (2)
Agmo Hernández, Víct ... (2)
Ohlson, Sten (2)
Bergström, Maria (2)
Eriksson, Jonny (2)
Duong-Thi, Minh-Dao (2)
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To Yiu Ying, Janet (2)
Torres, Jaume (2)
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University
Uppsala University (1)
Linnaeus University (1)
Language
English (2)
Research subject (UKÄ/SCB)
Natural sciences (2)
Year

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