| 1. |
- Kehoe, Laura, et al.
(författare)
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Make EU trade with Brazil sustainable
- 2019
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Ingår i: Science. - : American Association for the Advancement of Science (AAAS). - 0036-8075 .- 1095-9203. ; 364:6438, s. 341-
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Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)
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| 2. |
- Beal, Jacob, et al.
(författare)
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Robust estimation of bacterial cell count from optical density
- 2020
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Ingår i: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
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Tidskriftsartikel (refereegranskat)abstract
- Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
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| 3. |
- Lembrechts, Jonas J., et al.
(författare)
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SoilTemp : A global database of near-surface temperature
- 2020
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Ingår i: Global Change Biology. - : Wiley. - 1354-1013 .- 1365-2486. ; 26:11, s. 6616-6629
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Tidskriftsartikel (refereegranskat)abstract
- Current analyses and predictions of spatially explicit patterns and processes in ecology most often rely on climate data interpolated from standardized weather stations. This interpolated climate data represents long-term average thermal conditions at coarse spatial resolutions only. Hence, many climate-forcing factors that operate at fine spatiotemporal resolutions are overlooked. This is particularly important in relation to effects of observation height (e.g. vegetation, snow and soil characteristics) and in habitats varying in their exposure to radiation, moisture and wind (e.g. topography, radiative forcing or cold-air pooling). Since organisms living close to the ground relate more strongly to these microclimatic conditions than to free-air temperatures, microclimatic ground and near-surface data are needed to provide realistic forecasts of the fate of such organisms under anthropogenic climate change, as well as of the functioning of the ecosystems they live in. To fill this critical gap, we highlight a call for temperature time series submissions to SoilTemp, a geospatial database initiative compiling soil and near-surface temperature data from all over the world. Currently, this database contains time series from 7,538 temperature sensors from 51 countries across all key biomes. The database will pave the way toward an improved global understanding of microclimate and bridge the gap between the available climate data and the climate at fine spatiotemporal resolutions relevant to most organisms and ecosystem processes.
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| 4. |
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| 5. |
- Agemark, Maria, et al.
(författare)
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Reconstitution of water channel function and 2D-crystallization of human aquaporin 8.
- 2012
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Ingår i: Biochimica et Biophysica Acta. - : Elsevier BV. - 0006-3002 .- 0005-2736. ; 1818:3, s. 839-850
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Tidskriftsartikel (refereegranskat)abstract
- Among the thirteen human aquaporins (AQP0-12), the primary structure of AQP8 is unique. By sequence alignment it is evident that mammalian AQP8s form a separate subfamily distinct from the other mammalian aquaporins. The constriction region of the pore determining the solute specificity deviates in AQP8 making it permeable to both ammonia and H(2)O(2) in addition to water. To better understand the selectivity and gating mechanism of aquaporins, high-resolution structures are necessary. So far, the structure of one human aquaporin (HsAQP5) has been solved at atomic resolution. For mammalian aquaporins in general, high-resolution structures are only available for those belonging to the water-specific subfamily (including HsAQP5). Thus, it is of interest to solve structures of other aquaporin subfamily members with different solute specificities. To achieve this the aquaporins need to be overexpressed heterologously and purified. Here we use the methylotrophic yeast Pichia pastoris as a host for the overexpression. A wide screen of different detergents and detergent-lipid combinations resulted in the solubilization of functional human AQP8 protein and in well-ordered 2D crystals. It also became evident that removal of amino acids constituting affinity tags was crucial to achieve highly ordered 2D crystals diffracting to 3Å.
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| 6. |
- Amare, Azmeraw T, et al.
(författare)
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Association of Polygenic Score for Schizophrenia and HLA Antigen and Inflammation Genes With Response to Lithium in Bipolar Affective Disorder: A Genome-Wide Association Study.
- 2018
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Ingår i: JAMA psychiatry. - : American Medical Association (AMA). - 2168-6238 .- 2168-622X. ; 75:1, s. 65-74
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Tidskriftsartikel (refereegranskat)abstract
- Lithium is a first-line mood stabilizer for the treatment of bipolar affective disorder (BPAD). However, the efficacy of lithium varies widely, with a nonresponse rate of up to 30%. Biological response markers are lacking. Genetic factors are thought to mediate treatment response to lithium, and there is a previously reported genetic overlap between BPAD and schizophrenia (SCZ).To test whether a polygenic score for SCZ is associated with treatment response to lithium in BPAD and to explore the potential molecular underpinnings of this association.A total of 2586 patients with BPAD who had undergone lithium treatment were genotyped and assessed for long-term response to treatment between 2008 and 2013. Weighted SCZ polygenic scores were computed at different P value thresholds using summary statistics from an international multicenter genome-wide association study (GWAS) of 36989 individuals with SCZ and genotype data from patients with BPAD from the Consortium on Lithium Genetics. For functional exploration, a cross-trait meta-GWAS and pathway analysis was performed, combining GWAS summary statistics on SCZ and response to treatment with lithium. Data analysis was performed from September 2016 to February 2017.Treatment response to lithium was defined on both the categorical and continuous scales using the Retrospective Criteria of Long-Term Treatment Response in Research Subjects with Bipolar Disorder score. The effect measures include odds ratios and the proportion of variance explained.Of the 2586 patients in the study (mean [SD] age, 47.2 [13.9] years), 1478 were women and 1108 were men. The polygenic score for SCZ was inversely associated with lithium treatment response in the categorical outcome, at a threshold P<5×10-2. Patients with BPAD who had a low polygenic load for SCZ responded better to lithium, with odds ratios for lithium response ranging from 3.46 (95% CI, 1.42-8.41) at the first decile to 2.03 (95% CI, 0.86-4.81) at the ninth decile, compared with the patients in the 10th decile of SCZ risk. In the cross-trait meta-GWAS, 15 genetic loci that may have overlapping effects on lithium treatment response and susceptibility to SCZ were identified. Functional pathway and network analysis of these loci point to the HLA antigen complex and inflammatory cytokines.This study provides evidence for a negative association between high genetic loading for SCZ and poor response to lithium in patients with BPAD. These results suggest the potential for translational research aimed at personalized prescribing of lithium.
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| 7. |
- Calabrese, Claudia, et al.
(författare)
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Genomic basis for RNA alterations in cancer
- 2020
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Ingår i: Nature. - : Springer Science and Business Media LLC. - 0028-0836 .- 1476-4687. ; 578:7793, s. 129-136
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Tidskriftsartikel (refereegranskat)abstract
- Transcript alterations often result from somatic changes in cancer genomes1. Various forms of RNA alterations have been described in cancer, including overexpression2, altered splicing3 and gene fusions4; however, it is difficult to attribute these to underlying genomic changes owing to heterogeneity among patients and tumour types, and the relatively small cohorts of patients for whom samples have been analysed by both transcriptome and whole-genome sequencing. Here we present, to our knowledge, the most comprehensive catalogue of cancer-associated gene alterations to date, obtained by characterizing tumour transcriptomes from 1,188 donors of the Pan-Cancer Analysis of Whole Genomes (PCAWG) Consortium of the International Cancer Genome Consortium (ICGC) and The Cancer Genome Atlas (TCGA)5. Using matched whole-genome sequencing data, we associated several categories of RNA alterations with germline and somatic DNA alterations, and identified probable genetic mechanisms. Somatic copy-number alterations were the major drivers of variations in total gene and allele-specific expression. We identified 649 associations of somatic single-nucleotide variants with gene expression in cis, of which 68.4% involved associations with flanking non-coding regions of the gene. We found 1,900 splicing alterations associated with somatic mutations, including the formation of exons within introns in proximity to Alu elements. In addition, 82% of gene fusions were associated with structural variants, including 75 of a new class, termed 'bridged' fusions, in which a third genomic location bridges two genes. We observed transcriptomic alteration signatures that differ between cancer types and have associations with variations in DNA mutational signatures. This compendium of RNA alterations in the genomic context provides a rich resource for identifying genes and mechanisms that are functionally implicated in cancer.
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| 8. |
- Doel, Ronald E., et al.
(författare)
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Strategic Arctic science : national interests in building natural knowledge - interwar era through the Cold War
- 2014
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Ingår i: Journal of Historical Geography. - : Elsevier BV. - 0305-7488 .- 1095-8614. ; 44, s. 60-80
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Tidskriftsartikel (refereegranskat)abstract
- From the 1930s through the 1950s-the decades bracketing the second and third international polar years research in the physical and biological environmental sciences of the Arctic increased dramatically. The heroic, expedition-based style of Arctic science, dominant in the first decades of the twentieth century, gave way to a systematic, long-term, strategic and largely statefunded model of research which increased both Arctic presence and the volume of research output. Factors that made this change possible were distinct for each of the five circumpolar nation-states considered here. For Soviet leaders, the Arctic was an untamed land containing vast economic resources, all within reach if its long-sought Northern Sea Route became reality; Soviet officials sought environmental knowledge of this region with a range of motivations from economic and strategic concerns to enhancing the prestige of socialism. In contrast, United States officials largely ignored the Arctic until the outbreak of World War II, when military commanders quickly grasped the strategic importance of this region. Anxious that the Arctic might become a literal battleground between East and West by 1947, as the Cold War began, Pentagon leaders funded vast northern research programs, including in strategically located Greenland. Canadian leaders while appreciating the national security concerns of its powerful southern neighbor were even more concerned with maintaining sovereignty over its northern territories and gaining knowledge to assist its northern economic ambitions. Norway and Sweden, as smaller states, faced distinct challenges. With strong claims to Arctic heritage but limited resources, leaders of these states sought to create independent research strategies while, especially in the case of Norway, protecting their geopolitical interests in relation to the Soviet Union and the U.S. This article provides the first internationally comparative study of the multiple economic, military, political, and strategic factors that motivated scientific activities and programs in the far north, from the interwar period through World War II and the Cold War, when carefully coordinated, station-based research programs were introduced. The production of knowledge about Arctic's physical environment including its changing climate had little resemblance either to ideas of science-based 'progress,' or responses to perceived environmental concerns. Instead, it demonstrates that strategic military, economic, geopolitical, and national security concerns influenced and shaped most science undertakings, including those of the International Polar Year of 1932-1933 and the following polar year, the International Geophysical Year of 1957-1958.
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| 9. |
- Eklund, Niklas, Docent, 1964-, et al.
(författare)
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Imageries and historical change in the European Russian Arctic
- 2019. - 1
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Ingår i: The politics of Arctic resources. - Abingdon : Routledge. - 9781138040601 - 9781315174969 ; , s. 200-220
-
Bokkapitel (refereegranskat)abstract
- Chapter 11 widens the Nordic focus of the book with an analysis of Russian policy imageries regarding its northern areas and their change over time. The chapter highlights different roles and conceptions of the North in Russia over time, laying the foundation for comparison and contrast with the Nordic countries. The chapter also highlights the current potential for integrated understandings and an inclusion of considerations amongst actors in the Russian northern areas.
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| 10. |
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| 11. |
- Gerth, Julia, et al.
(författare)
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Adhesion phenomena in the secondary shear zone in turning of austenitic stainless steel and carbon steel
- 2014
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Ingår i: Journal of Materials Processing Technology. - : Elsevier BV. - 0924-0136 .- 1873-4774. ; 214:8, s. 1467-1481
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Tidskriftsartikel (refereegranskat)abstract
- This paper aims to increase the understanding of the adhesion between chip and tool rake face by studying the initial material transfer to the tool during orthogonal machining at 150 m/min. Two types of work material were tested, an austenitic stainless steel, 316L, and a carbon steel, UHB 11. The tools used were cemented carbide inserts coated with hard ceramic coatings. Two different CVD coatings, TiN and Al2O3, produced with two different surface roughnesses, polished and rough, were tested. The influences of both tool surface topography and chemistry on the adhesion phenomena in the secondary shear zone were thus evaluated. Extensive surface analyses of the inserts after cutting were made using techniques such as Scanning Electron Microscopy (SEM), Energy Dispersive Spectroscopy (EDS), X-ray Photoelectron Spectroscopy (XPS), and Transmission Electron Microscopy (TEM). As expected, cutting in the stainless steel resulted in a higher amount of adhered material, compared to cutting in the carbon steel. Remnants of built-up layers were found on the surfaces of the 316L chips but not on the UHB 11 chips. Moreover, it was shown that for both materials the tool roughness had a profound effect, with the rougher surfaces comprising much higher amounts of adhered material than the polished ones. Non-metallic inclusions from both types of workpiece steels accumulate in the high temperature area on the inserts. The general tendency was that higher amounts of transferred material were found on the TiN coating than on the Al2O3 coating after cutting.
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| 12. |
- Gerth, Julia, et al.
(författare)
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Assessing the hardness and residual stress at the very edge of a TiAlN coated cutting insert
- 2024
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Ingår i: Surface and coatings technology. - : Elsevier. - 0257-8972.
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Tidskriftsartikel (refereegranskat)abstract
- A method for determining residual stresses through nanoindentation has been used in order to evaluate the local residual stress in a PVD Ti0.5Al0.5N coating. The influence of residual stress on the measured hardness is obtained by comparing the hardness of as deposited, residually stressed, coating and stress relieved pillars made in the very same coating. The technique offers high lateral resolution, and it can be applied on complex geometries where conventional stress measurements fall short. In this work, the method proved useful for estimating the residual stress at such a complex geometry as a sharp cutting edge. Edges with two different radii were analysed and it was concluded that the nominal residual compressive stress given to a coating can locally, on the very cutting edge, become significantly reduced by elastic relaxation .This effects the cohesion of the coating at that position which, together with the lift-off stress generated at the edge, make the coating in that region especially vulnerable to damage. The experimental results correlated very well with finite element simulations of the residual stress state around cutting edges with the same geometries.
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| 13. |
- Gerth, Julia, et al.
(författare)
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Evaluation of an intermittent sliding test for reproducing work material transfer in milling operations
- 2012
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Ingår i: Tribology International. - : Elsevier BV. - 0301-679X .- 1879-2464. ; 52, s. 153-160
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Tidskriftsartikel (refereegranskat)abstract
- TiN coated HSS test cylinders from an intermittent sliding test were compared with TiN coated HSS milling inserts from a single insert milling test. A 20NiCrMo2 case hardening steel was used as counter material and work material in the two tests. HSS test cylinders, coated with AlCrN, TiAlN and Al2O3, were also tested in the intermittent sliding test and evaluated with regard to material adhesion. Two distinctly different tribofilms were formed on test cylinders as well as on cutting inserts. One consisting of Mn, Si, Al and O and one consisting of Fe, Mn, Cr, Si and O. The transferred material was similar in appearance and composition, both when comparing the two test methods and when comparing the different tool coatings.
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| 14. |
- Gerth, Julia Lundberg
(författare)
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Tribology at the Cutting Edge : A Study of Material Transfer and Damage Mechanisms in Metal Cutting
- 2012
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Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- The vision of this thesis is to improve the metal cutting process, with emphasis on the cutting tool, to enable stable and economical industrial production while using expensive tools such as hobs. The aim is to increase the tribological understanding of the mechanisms operating at a cutting edge and of how these can be controlled using different tool parameters. Such understanding will facilitate the development and implementation of future, tribologically designed, cutting tools.Common wear and failure mechanisms in gear hobbing have been identified and focused studies of the material transferred to the tool, in both metal cutting operations and in simplified tribological tests, have been conducted. Interactions between residual stresses in the tool coating and the shape of the cutting edge have also been studied.It was concluded that tool failure is often initiated via small defects in the coated tool system, and it is necessary to eliminate, or minimize, these defects in order to manufacture more reliable and efficient gear cutting tools. Furthermore, the geometry of a cutting edge should be optimized with the residual stress state in the coating, in mind. The interaction between a compressive stress and the geometry of the cutting edge will affect the stress state at the cutting edge and thus affect the practical toughness and the wear resistance of the coating in that area.An intermittent sliding contact test is presented and shown to be of high relevance for studying the interaction between the tool rake face and the chip in milling. It was also demonstrated that material transfer, that can have large effects on the cutting performance, commences already after very short contact times. The nature of the transfer may differ in different areas on the tool. It may include glassy layers, with accumulations of specific elements from the workpiece, and transfer of steel in more or less oxidized form. Both tool coating material, its surface roughness, and the relative speed between the tool surface and the chip, may influence the extent to which the different transfer will occur.
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| 15. |
- Gerth, Julia, 1979-, et al.
(författare)
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On the wear of PVD-coated HSS hobs in dry gear cutting
- 2009
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Ingår i: Wear. - : Elsevier BV. - 0043-1648 .- 1873-2577. ; 266:3-4, s. 444-452
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Tidskriftsartikel (refereegranskat)abstract
- A hob is an advanced gear cutting tool usually made of homogenous high-speed steel (HSS) and coated by physical vapour deposition (PVD). It is designed for regrinding and recoating many times. However, hobs of today suffer from unpredictable wear making it difficult to schedule when they should be taken out for reconditioning. This investigation is aimed to contribute to the fundamental knowledge of the wear mechanisms and wear propagation of hobs used in dry gear cutting. Two AlCrN-coated hobs, used in actual gear cutting, were investigated by scanning electron microscopy (SEM) to study the tool topography, light optical microscopy (LOM) to study metallographic cross-sections and by X-ray photoelectron spectroscopy (XPS) for surface analyses.It is concluded that the high potential of PVD-coated HSS as tool material is not taken full advantage of. The main reason is a combination of a poor surface preparation prior to coating and excessively high compressive residual stresses, making parts of the coating detach along the edge line even on unused cutting teeth. During cutting, the high intrinsic stresses in combination with droplets and defects in the coating facilitated its fragmentation through decohesion and detachment. Simultaneously, the rake face is gradually covered by a thin oxide layer dominated by Fe, Cr and Mn in about equal amounts, and also with a significant content of Si. It is believed but not proved that this layer is beneficial.To improve service life and reliability of gear cutting hobs, precautions should be taken for the grinding–polishing preparation prior to PVD-coating to ensure a smooth substrate free from burning damage and other defects. Also, the edge radius and coating thickness has to be matched with the magnitude of compressive residual stresses in the coating.
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| 16. |
- Gerth, Julia, 1979-, et al.
(författare)
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Reproducing wear mechanisms in gear hobbing : Evaluation of a single insert milling test
- 2009
-
Ingår i: Wear. - : Elsevier BV. - 0043-1648 .- 1873-2577. ; 267:12, s. 2257-2268
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Tidskriftsartikel (refereegranskat)abstract
- Gear hobbing is a widely used method in industrial gear manufacturing. The most common type of hob is made of homogenous HSS and protected by a PVD coating. In order to increase the reliability and tool life of these milling tools, further developments of the tool surfaces and cutting edges are necessary. A single tooth milling test, using a HSS insert in a conventional milling machine, has been developed with the aim to reproduce the wear mechanisms seen on real HSS gear hobbing teeth. The benefits of such a test, compared to actual gear hobbing tests, are primarily accessibility and reduced costs for both design and production of test specimens (inserts). The main goal of this study was to verify that the wear mechanisms in the developed test correspond with the wear mechanisms obtained in real gear hobbing. Once this was verified, the influence of surfaces roughness on the performance of TiAlN coated HSS inserts was evaluated by using the tool as delivered or after polishing the tool surfaces. Parameters considered were tool wear, cutting forces and the quality of machined surfaces. The polished inserts, yielded less adhered work material and reduced flank wear but no significant difference in cutting forces as compared to the unpolished inserts.
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| 17. |
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| 18. |
- Gerth, Julia, et al.
(författare)
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Survey of damage mechanisms on PVD coated HSS hobs used in Swedish gear manufacturing industry
- 2011
-
Ingår i: Tribologia. - 0780-2285. ; 30:1-2, s. 37-50
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Tidskriftsartikel (refereegranskat)abstract
- Gear hobbing is widely used for production of cylindrical gears in the Swedish transmission industry. The hob, usually consisting of a homogenous HSS (High Speed Steel) body coated with a ceramic PVD (Physical Vapour Deposition) coating, is designed for regrinding and recoating several times without affecting its cutting geometries. Efficient usage of the tool, considering production costs and gear quality, requires reconditioning before wear starts to affect the gear quality negatively and certainly before tool wear renders reconditioning impossible. Hobs of today generally lack in reliability, making it difficult to judge when they have to be taken out for reconditioning. This work presents a survey of wear as observed on today’s state of the art hobs used by Swedish gear manufactures. It aims to identify damage mechanisms and the common problems in order to enable future production of more reliable hobs. The tools were temporarily borrowed from the production and the analyses were made non-destructively using optical microscopes. This was complemented by destructive cross-sectional analysis on two of the hobs. Wear was most commonly located on the rake faces and the cutting edges of the cutting teeth. It mainly propagates by discrete fractures which appear to originate at local defects in the coating or at the interface. High intrinsic stress in the coating likely promotes coating spallation and accelerates the wear of the cutting edge.
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| 19. |
- Gerth, Julia, et al.
(författare)
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The influence of metallic interlayers on the adhesion of pVD TiN coatings on high-speed steel
- 2007
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Ingår i: Wear. - : Elsevier BV. - 0043-1648 .- 1873-2577. ; 264:9-10, s. 885-892
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Tidskriftsartikel (refereegranskat)abstract
- In nearly all applications the adhesion of the coating to the substrate is crucial for the components performance and length of life. To enhance the adhesion it is common to use a metallic interlayer, most often titanium. In this study seven different metallic interlayers, namely W, Mo, Nb, Cr, Ti, Ag and Al, have been evaluated with respect to their influence on the adhesion of PVD TiN coatings to polished high-speed steel, ASP 2060. The purpose of this work is to investigate how some physical properties of a metal affect its capability to function as an adhesion interlayer. Samples were prepared using dc magnetron sputtering for the interlayer and reactive dc magnetron sputtering for the TiN coating. The deposition process included both pre-treatments and in situ treatments of the substrate surface in order to eliminate possible contaminations. The adhesion of the coatings was investigated with two different methods: scratch testing and Rockwell adhesion testing. The results indicate that differences in hardness between the metallic interlayers influence the practical adhesion more than differences in E-modulus. Furthermore, in order to optimize adhesion, the hardness of the interlayer should be close to the hardness of the substrate. It was also suggested that stresses, both in the TiN coating and in the metallic interlayer, affect the adhesion properties negatively. In addition, the necessity of interlayer in TiN on HSS can be questioned as the reference samples, without interlayer, showed adhesion properties comparable to the highest ranked interlayer containing samples in our assessment.
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| 20. |
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| 21. |
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| 22. |
- Heinrichs, Jannica, et al.
(författare)
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Influence from surface roughness on steel transfer to PVD tool coatings in continuous and intermittent sliding contacts
- 2012
-
Ingår i: Tribology International. - : Elsevier BV. - 0301-679X .- 1879-2464. ; 56, s. 9-18
-
Tidskriftsartikel (refereegranskat)abstract
- A sliding test is used in order to evaluate the influence of tool surface roughness on the material transfer in intermittent and continuous sliding of PVD coated HSS against case hardening steel (20NiCrMo2). Two cutting tool coatings, TiN and AlCrN, and three different surface roughnesses are tested. For polished surfaces the same types of material transfer are obtained irrespective of sliding mode and coating type. If the surfaces are too rough, the tribofilms do not grow thick enough to separate the surfaces and the work material is abrasively worn in both sliding modes. With increased sliding distance, cracking of the TiN coating occurs while the AlCrN coating remains intact.
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| 23. |
- Heinrichs, Jannica, et al.
(författare)
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Influence of sliding speed on modes of material transfer as steel slides against PVD tool coatings
- 2013
-
Ingår i: Tribology International. - : Elsevier. - 0301-679X .- 1879-2464. ; 58, s. 55-64
-
Tidskriftsartikel (refereegranskat)abstract
- An intermittent sliding test was used in order to study the formation and build- up of tribofilms during intermittent sliding of PVD coated HSS against case hardening steel (20NiCrMo2). Two cutting tool coatings were tested, TiN and AlCrN, and the influence of sliding speed was evaluated. With moderate speed, two tribofilms were formed separately, one consisting of Mn, Si, Al and O on an intermediate layer of Fe and one consisting of Fe, Mn, Cr and O on an intermediate layer of Cr and Mn. At low sliding speeds an uneven transfer of steel occured while high sliding speeds resulted in thermal softening of the substrate leading to coating failure. AlCrN provided better substrate protection at high speeds than TiN did.
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| 24. |
- Henz Ryen, Astrid
(författare)
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Bisabolane sesquiterpenes from the leaves of Lindera benzoin reduce prostaglandin E2 formation in A549 cells
-
Ingår i: Phytochemistry Letters. - 1874-3900 .- 1876-7486.
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Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)abstract
- Phytochemical investigation of leaves from the American shrub Lindera benzoin (L.) Blume (Lauraceae) resulted in the isolation of one pure compound (1) and a diastereomeric mixture of (2 and 3). The structures of these new bisabolane sesquiterpenes were elucidated via MS and extensive NMR measurements and identified as 6-(2-hydroxy-6-methylhept-5-en-2-yl)-3-(hydroxymethyl)-4-oxocyclohex-2-en-1-yl acetate (1) and 3-(hydroxymethyl)-6-(5-(2-hydroxypropan-2-yl)-2-methyltetrahydrofuran-2-yl)-4-oxocyclohex-2-en-1-yl acetate (2 and 3). The compounds were evaluated in vitro for their anti-inflammatory activity. In cellular assays, 1-3 reduced pro-inflammatory prostaglandin E2 production in A549 cells in a dose-dependent manner.
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| 25. |
- Henz Ryen, Astrid, et al.
(författare)
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Bisabolane sesquiterpenes from the leaves of Lindera benzoin reduce prostaglandin E2 formation in A549 cells
- 2020
-
Ingår i: Phytochemistry Letters. - : ELSEVIER. - 1874-3900 .- 1876-7486. ; 38, s. 6-11
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Tidskriftsartikel (refereegranskat)abstract
- Phytochemical investigation of leaves from the American shrub Lindera benzoin (L.) Blume (Lauraceae) resulted in the isolation of one pure compound 1 and a diastereomeric mixture of 2 and 3. The structures of these new bisabolane sesquiterpenes were elucidated via MS and extensive NMR measurements and identified as 6-(2hydroxy-6-methylhept-5-en-2-yl)-3-(hydroxymethyl)-4-oxocyclohex-2-en-1-yl acetate (1) and 3-(hydroxymethyl)-6-(5-(2-hydroxypropan-2-yl)-2-methyltetrahydrofuran-2-yl)-4-oxocyclohex-2-en-1-yl acetate (2 and 3). The compounds were evaluated in vitro for their anti-inflammatory activity. In cellular assays, 1-3 reduced pro-inflammatory prostaglandin E2 production in A549 cells in a dose-dependent manner.
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| 26. |
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| 27. |
- Hou, Liping, et al.
(författare)
-
Genome-wide association study of 40,000 individuals identifies two novel loci associated with bipolar disorder.
- 2016
-
Ingår i: Human molecular genetics. - : Oxford University Press (OUP). - 1460-2083 .- 0964-6906. ; 25:15, s. 3383-94
-
Tidskriftsartikel (refereegranskat)abstract
- Bipolar disorder (BD) is a genetically complex mental illness characterized by severe oscillations of mood and behavior. Genome-wide association studies (GWAS) have identified several risk loci that together account for a small portion of the heritability. To identify additional risk loci, we performed a two-stage meta-analysis of >9 million genetic variants in 9,784 bipolar disorder patients and 30,471 controls, the largest GWAS of BD to date. In this study, to increase power we used ∼2,000 lithium-treated cases with a long-term diagnosis of BD from the Consortium on Lithium Genetics, excess controls, and analytic methods optimized for markers on the X-chromosome. In addition to four known loci, results revealed genome-wide significant associations at two novel loci: an intergenic region on 9p21.3 (rs12553324, p=5.87×10(-9); odds ratio=1.12) and markers within ERBB2 (rs2517959, p=4.53×10(-9); odds ratio=1.13). No significant X-chromosome associations were detected and X-linked markers explained very little BD heritability. The results add to a growing list of common autosomal variants involved in BD and illustrate the power of comparing well-characterized cases to an excess of controls in GWAS.
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| 28. |
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| 29. |
- Kalman, Janos L, et al.
(författare)
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Investigating polygenic burden in age at disease onset in bipolar disorder: Findings from an international multicentric study.
- 2019
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Ingår i: Bipolar disorders. - : Wiley. - 1399-5618 .- 1398-5647. ; 21:1, s. 68-75
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Tidskriftsartikel (refereegranskat)abstract
- Bipolar disorder (BD) with early disease onset is associated with an unfavorable clinical outcome and constitutes a clinically and biologically homogenous subgroup within the heterogeneous BD spectrum. Previous studies have found an accumulation of early age at onset (AAO) in BD families and have therefore hypothesized that there is a larger genetic contribution to the early-onset cases than to late onset BD. To investigate the genetic background of this subphenotype, we evaluated whether an increased polygenic burden of BD- and schizophrenia (SCZ)-associated risk variants is associated with an earlier AAO in BD patients.A total of 1995 BD type 1 patients from the Consortium of Lithium Genetics (ConLiGen), PsyCourse and Bonn-Mannheim samples were genotyped and their BD and SCZ polygenic risk scores (PRSs) were calculated using the summary statistics of the Psychiatric Genomics Consortium as a training data set. AAO was either separated into onset groups of clinical interest (childhood and adolescence [≤18years] vs adulthood [>18years]) or considered as a continuous measure. The associations between BD- and SCZ-PRSs and AAO were evaluated with regression models.BD- and SCZ-PRSs were not significantly associated with age at disease onset. Results remained the same when analyses were stratified by site of recruitment.The current study is the largest conducted so far to investigate the association between the cumulative BD and SCZ polygenic risk and AAO in BD patients. The reported negative results suggest that such a polygenic influence, if there is any, is not large, and highlight the importance of conducting further, larger scale studies to obtain more information on the genetic architecture of this clinically relevant phenotype.
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| 30. |
- Kelsoe, John, et al.
(författare)
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Lithium Response in Bipolar Disorder is Associated with Focal Adhesion and PI3K-Akt Networks: A Multi-omics Replication Study.
- 2023
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Ingår i: Research square.
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Annan publikation (övrigt vetenskapligt/konstnärligt)abstract
- Lithium is the gold standard treatment for bipolar disorder (BD). However, its mechanism of action is incompletely understood, and prediction of treatment outcomes is limited. In our previous multi-omics study of the Pharmacogenomics of Bipolar Disorder (PGBD) sample combining transcriptomic and genomic data, we found that focal adhesion, the extracellular matrix (ECM), and PI3K-Akt signaling networks were associated with response to lithium. In this study, we replicated the results of our previous study using network propagation methods in a genome-wide association study of an independent sample of 2,039 patients from the International Consortium on Lithium Genetics (ConLiGen) study. We identified functional enrichment in focal adhesion and PI3K-Akt pathways, but we did not find an association with the ECM pathway. Our results suggest that deficits in the neuronal growth cone and PI3K-Akt signaling, but not in ECM proteins, may influence response to lithium in BD.
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| 31. |
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| 32. |
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| 33. |
- Nyberg, Harald, 1985-, et al.
(författare)
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On the influence from micro topography on the structure and growth of low friction amorphous carbon PVD coatings
- 2010
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Ingår i: Twelfth International Conference on Plasma Surface Engineering (PSE 2010), September 13-17, 2010, Garmisch-Partenkirchen.
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Konferensbidrag (refereegranskat)abstract
- The interest in metal carbide doped amorphous carbon coatings produced by physical vapour deposition (PVD) for use on heavily stressed machine elements is currently increasing, mainly due to their ability to achieve low friction and reduced wear of the counter surface. The tribological properties of these types of coatings have however been found to vary strongly between seemingly similar coatings. A potential source of these differences could be the micro topography of the coated surfaces. Argon ion etch cleaning of the substrates is a common process step in the production of PVD coatings and is usually performed as the final cleaning step before coating deposition. For some materials, the etching process may result in a roughening of the substrate surface, due to differences in the etch rates of the different parts of the material. In high speed steels, carbides typically etch slower than the metallic phase, resulting in a surface covered by protruding carbides. In the current study, it was examined how varying amounts of argon ion etching of highly polished high speed steel substrates prior to coating influences the micro topography of the substrates and the final coatings. Tantalum carbide doped amorphous carbon coatings (TaC:C) were produced by co-sputtering of carbon and tantalum, in an argon atmosphere. The impact of the substrate micro topography on the growth and structure of the coatings was studied, using high resolution scanning electron microscopy (SEM) of superficial coating cross sections produced with a focused ion beam (FIB). Special attention was paid to coating growth in the immediate vicinity of protruding carbides, as well as to the structure of the coating in these regions.
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| 34. |
- Olofsson, Johanna, 1981-, et al.
(författare)
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On the influence from micro topography of PVD coatings on friction behaviour, material transfer and tribofilm formation
- 2011
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Ingår i: Wear. - : Elsevier. - 0043-1648 .- 1873-2577. ; 271:9-10, s. 204-2057
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Konferensbidrag (refereegranskat)abstract
- PVD coatings based on amorphous carbon with metal-carbides are currently gaining a high interest for use on machine elements due to their potential to give low friction and low wear of the counter surface. However, the performance varies significantly between the various types of such coatings and the causes of this are not clear. One factor is the micro topography of the coating surface. This topography may influence the friction in many ways; by changing the state of lubrication, by causing scratching of the counter surface, by modifying the topography of the counter surface the material transfer, the tribofilm formation, etc.TaC/a-C coatings, produced by co-sputtering of carbon and tantalum in an argon atmosphere, were deposited on high speed steel substrates exposed to varying degrees of etching to produce a range of surface roughnesses. Ball-on-disc experiments were used to evaluate the tribological properties of the coatings in dry condition against a ball bearing steel ball. The surfaces were analysed using various advanced techniques, including, SEM, XPS, Raman, EDS and AFM, all both prior to and after the testing.It was shown that the resulting surface topography of the coating is affected even by very small protrusions on the substrate. The coefficient of friction decreased during use to a stable level, due to a complex process including tribofilm build-up on the sliding ball. Surfaces with lower protrusions exhibited a faster friction decrease, i.e. a faster running in.
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| 35. |
- Ou, Anna H., et al.
(författare)
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Lithium response in bipolar disorder is associated with focal adhesion and PI3K-Akt networks: a multi-omics replication study
- 2024
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Ingår i: TRANSLATIONAL PSYCHIATRY. - 2158-3188. ; 14:1
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Tidskriftsartikel (refereegranskat)abstract
- Lithium is the gold standard treatment for bipolar disorder (BD). However, its mechanism of action is incompletely understood, and prediction of treatment outcomes is limited. In our previous multi-omics study of the Pharmacogenomics of Bipolar Disorder (PGBD) sample combining transcriptomic and genomic data, we found that focal adhesion, the extracellular matrix (ECM), and PI3K-Akt signaling networks were associated with response to lithium. In this study, we replicated the results of our previous study using network propagation methods in a genome-wide association study of an independent sample of 2039 patients from the International Consortium on Lithium Genetics (ConLiGen) study. We identified functional enrichment in focal adhesion and PI3K-Akt pathways, but we did not find an association with the ECM pathway. Our results suggest that deficits in the neuronal growth cone and PI3K-Akt signaling, but not in ECM proteins, may influence response to lithium in BD.
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| 36. |
- Speetjens, Niek Jesse, et al.
(författare)
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Dissolved organic matter characterization in soils and streams in a small coastal low-Arctic catchment
- 2022
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Ingår i: Biogeosciences. - : Copernicus GmbH. - 1726-4170 .- 1726-4189. ; 19:12, s. 3073-3097
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Tidskriftsartikel (refereegranskat)abstract
- Ongoing climate warming in the western Canadian Arctic is leading to thawing of permafrost soils and subsequent mobilization of its organic matter pool. Part of this mobilized terrestrial organic matter enters the aquatic system as dissolved organic matter (DOM) and is laterally transported from land to sea. Mobilized organic matter is an important source of nutrients for ecosystems, as it is available for microbial breakdown, and thus a source of greenhouse gases. We are beginning to understand spatial controls on the release of DOM as well as the quantities and fate of this material in large Arctic rivers. Yet, these processes remain systematically understudied in small, high-Arctic watersheds, despite the fact that these watersheds experience the strongest warming rates in comparison. Here, we sampled soil (active layer and permafrost) and water (porewater and stream water) from a small ice wedge polygon (IWP) catchment along the Yukon coast, Canada, during the summer of 2018. We assessed the organic carbon (OC) quantity (using dissolved (DOC) and particulate OC (POC) concentrations and soil OC content), quality (δ13C DOC, optical properties and source apportionment) and bioavailability (incubations; optical indices such as slope ratio, Sr; and humification index, HIX) along with stream water properties (temperature, T; pH; electrical conductivity, EC; and water isotopes). We classify and compare different landscape units and their soil horizons that differ in microtopography and hydrological connectivity, giving rise to differences in drainage capacity. Our results show that porewater DOC concentrations and yield reflect drainage patterns and waterlogged conditions in the watershed. DOC yield (in mg DOC g−1 soil OC) generally increases with depth but shows a large variability near the transition zone (around the permafrost table). Active-layer porewater DOC generally is more labile than permafrost DOC, due to various reasons (heterogeneity, presence of a paleo-active-layer and sampling strategies). Despite these differences, the very long transport times of porewater DOC indicate that substantial processing occurs in soils prior to release into streams. Within the stream, DOC strongly dominates over POC, illustrated by DOC/POC ratios around 50, yet storm events decrease that ratio to around 5. Source apportionment of stream DOC suggests a contribution of around 50 % from permafrost/deep-active-layer OC, which contrasts with patterns observed in large Arctic rivers (12 ± 8 %; Wild et al., 2019). Our 10 d monitoring period demonstrated temporal DOC patterns on multiple scales (i.e., diurnal patterns, storm events and longer-term trends), underlining the need for high-resolution long-term monitoring. First estimates of Black Creek annual DOC (8.2 ± 6.4 t DOC yr−1) and POC (0.21 ± 0.20 t yr−1) export allowed us to make a rough upscaling towards the entire Yukon Coastal Plain (34.51 ± 2.7 kt DOC yr−1 and 8.93 ± 8.5 kt POC yr−1). Rising Arctic temperatures, increases in runoff, soil organic matter (OM) leaching, permafrost thawing and primary production are likely to increase the net lateral OC flux. Consequently, altered lateral fluxes may have strong impacts on Arctic aquatic ecosystems and Arctic carbon cycling.
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| 37. |
- Östgren, Carl Johan, et al.
(författare)
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Prevalence of atherosclerosis in individuals with prediabetes and diabetes compared to normoglycaemic individuals-a Swedish population-based study.
- 2023
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Ingår i: Cardiovascular diabetology. - : BioMed Central (BMC). - 1475-2840. ; 22:1
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Tidskriftsartikel (refereegranskat)abstract
- Patients with type 2 diabetes have an increased risk of death and cardiovascular events and people with diabetes or prediabetes have been found to have increased atherosclerotic burden in the coronary and carotid arteries. This study will estimate the cross-sectional prevalence of atherosclerosis in the coronary and carotid arteries in individuals with prediabetes and diabetes, compared with normoglycaemic individuals in a large population-based cohort.The 30,154 study participants, 50-64years, were categorized according to their fasting glycaemic status or self-reported data as normoglycaemic, prediabetes, and previously undetected or known diabetes. Prevalence of affected coronary artery segments, severity of stenosis and coronary artery calcium score (CACS) were determined by coronary computed tomography angiography. Total atherosclerotic burden was assessed in the 11 clinically most relevant segments using the Segment Involvement Score and as the presence of any coronary atherosclerosis. The presence of atherosclerotic plaque in the carotid arteries was determined by ultrasound examination.Study participants with prediabetes (n=4804, 16.0%) or diabetes (n=2282, 7.6%) had greater coronary artery plaque burden, more coronary stenosis and higher CACS than normoglycaemic participants (all, p<0.01). Among male participants with diabetes 35.3% had CACS≥100 compared to 16.1% among normoglycaemic participants. For women, the corresponding figures were 8.9% vs 6.1%. The prevalence of atherosclerosis in the coronary arteries was higher in participants with previously undetected diabetes than prediabetes, but lower than in patients with known diabetes. The prevalence of any plaque in the carotid arteries was higher in participants with prediabetes or diabetes than in normoglycaemic participants.In this large population-based cohort of currently asymptomatic people, the atherosclerotic burden in the coronary and carotid arteries increased with increasing degree of dysglycaemia. The finding that the atherosclerotic burden in the coronary arteries in the undetected diabetes category was midway between the prediabetes category and patients with known diabetes may have implications for screening strategies and tailored prevention interventions for people with dysglycaemia in the future.
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