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- O'Maille, G, et al.
(författare)
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Metabolomics relative quantitation with mass spectrometry using chemical derivatization and isotope labeling
- 2008
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Ingår i: SPECTROSCOPY-AN INTERNATIONAL JOURNAL. - : Hindawi Limited. - 0712-4813. ; 22:5, s. 327-343
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Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)abstract
- Comprehensive detection and quantitation of metabolites from a biological source constitute the major challenges of current metabolomics research. Two chemical derivatization methodologies, butylation and amination, were applied to human serum for ionization enhancement of a broad spectrum of metabolite classes, including steroids and amino acids. LC-ESI-MS analysis of the derivatized serum samples provided a significant signal elevation across the total ion chromatogram to over a 100-fold increase in ionization efficiency. It was also demonstrated that derivatization combined with isotopically labeled reagents facilitated the relative quantitation of derivatized metabolites from individual as well as pooled samples.
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