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- Gegzna, V., et al.
(författare)
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Methods for autofluorescence analysis of uterine cavity washings
- 2015
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Ingår i: Lithuanian Journal of Physics. - : Lithuanian Academy of Sciences. - 1648-8504. ; 55:1, s. 63-70
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Tidskriftsartikel (refereegranskat)abstract
- The diagnostics of endometrial pathology can be done by obtaining information at the molecular level, e. g. using the autofluorescence-based technique. Thus, for the first time an experimental study was performed on waste material of uterine cavity washing specimens to evaluate suitability of the technique for diagnostics. The specimens were obtained from 32 patients who had a conventional uterine hydrosonography procedure. A portable Nd:YAG 355 nm microlaser was used to excite autofluorescence at the point of care. Various algorithms of multivariate curve resolution and artificial neural networks were utilized for spectra analysis. The spectra were classified according to histological and ultrasound diagnosis. Receiver operating characteristic (ROC) curve analysis was used to make statistical decisions. The results showed that it was possible to distinguish all compared groups: pathologic vs non-pathologic endometrium (sensitivity 97.3 +/- 5.2%, specificity 91.7 +/- 7%, AUC (area under the ROC curve) 0.96 +/- 0.04), malignant endometrium vs endometrial polyps (sensitivity 100 +/- 0%, specificity 92.0 +/- 10.6%, AUG = 0.98 +/- 0.07), and secretory menstrual cycle phase vs proliferative phase (sensitivity 87.5 +/- 13.2%, specificity 94.4 +/- 7.4%, AUG = 0.88 +/- 0.10). To conclude, uterine cavity washing specimens could be used for endometrial pathology recognition using the autofluorescence-based technique in clinical setting. It will possibly speed up the treatment decision making for endometrial pathology.
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| 4. |
- Pålsson, Sara, et al.
(författare)
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Methods for detailed histopathological investigation and localization of biopsies from cervix uteri to improve the interpretation of auto fluorescence data
- 2006
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Ingår i: Journal of Environmental Pathology and Toxicology. - 2162-6537. ; 25:1-2, s. 321-340
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Tidskriftsartikel (refereegranskat)abstract
- Fluorescence spectroscopy is one of many optical methods that are potentially clinically useful for noninvasive detection and characterization of disorders on the cervical part of uterus, including precancerous lesions. The cervix uteri exhibits a biologically complex tissue and the morphology of a biopsy is generally not homogenous. The standard histopathological protocol accounts only for the most severe condition found within the biopsy and no information is given on other constituents potentially influencing the recorded fluorescence spectra. Spectra are usually correlated, using multivariate techniques, to the histopathological diagnosis of the biopsies. Since the probe volume of fluorescence spectroscopy is considerably smaller than the extension of the biopsy, this can cause problems in the search for correlation between the fluorescence signals and the pathological structures. In addition, the orientation and location of the biopsies are normally not recorded. We now report on the first detailed histopathological protocol where numerous tissue parameters, such as thickness and type of the epithelium and the number of blood vessels, glands, and inflammatory cells, are tabulated and the orientation and location of the biopsy are recorded as precisely as possible. Hopefully, the use of this protocol together with sophisticated mathematical methods will increase the probability to classify cervical disorders of the uterus, including precancerous lesions, with high sensitivity and specificity.
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