| 1. |
- Hu, Jiandong, et al.
(författare)
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Development of a Surface Plasmon Resonance Biosensing Approach for the Rapid Detection of Porcine Circovirus Type2 in Sample Solutions
- 2014
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Ingår i: PLOS ONE. - : Public Library of Science (PLoS). - 1932-6203. ; 9:10, s. e111292-
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Tidskriftsartikel (refereegranskat)abstract
- A sensitive and label-free analytical approach for the detection of porcine circovirus type 2 (PCV2) instead of PCV2 antibody in serum sample was systematically investigated in this research based on surface plasmon resonance (SPR) with an establishment of special molecular identification membrane. The experimental device for constructing the biosensing analyzer is composed of an integrated biosensor, a home-made microfluidic module, and an electrical control circuit incorporated with a photoelectric converter. In order to detect the PCV2 using the surface plasmon resonance immunoassay, the mercaptopropionic acid has been used to bind the Au film in advance through the known form of the strong S-Au covalent bonds formed by the chemical radical of the mercaptopropionic acid and the Au film. PCV2 antibodies were bonded with the mercaptopropionic acid by covalent -CO-NH- amide bonding. For the purpose of evaluating the performance of this approach, the known concentrations of PCV2 Cap protein of 10 mu g/mL, 7.5 mu g/mL, 5 mu g/mL, 2.5 mu g/mL, 1 mu g/mL, and 0.5 mu g/mL were prepared by diluting with PBS successively and then the delta response units (Delta RUs) were measured individually. Using the data collected from the linear CCD array, the Delta RUs gave a linear response over a wide concentration range of standard known concentrations of PCV2 Cap protein with the R-Squared value of 0.99625. The theoretical limit of detection was calculated to be 0.04 mu g/mL for the surface plasmon resonance biosensing approach. Correspondingly, the recovery rate ranged from 81.0% to 89.3% was obtained. In contrast to the PCV2 detection kits, this surface plasmon resonance biosensing system was validated through linearity, precision and recovery, which demonstrated that the surface plasmon resonance immunoassay is reliable and robust. It was concluded that the detection method which is associated with biomembrane properties is expected to contribute much to determine the PCV2 in sample solutions instead of PCV2 antibody in serum samples quantitatively.
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| 2. |
- Zhu, Liping, et al.
(författare)
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A similar to 30,000-year record of environmental changes inferred from Lake Chen Co, Southern Tibet
- 2009
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Ingår i: Journal of Paleolimnology. - : Springer Science and Business Media LLC. - 0921-2728 .- 1573-0417. ; 42:3, s. 343-358
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Tidskriftsartikel (refereegranskat)abstract
- Climatic and environmental changes since the last glacial period are important to our understanding of global environmental change. There are few records from Southern Tibet, one of the most climatically sensitive areas on earth. Here we present a study of the lake sediments (TC1 core) from Lake Chen Co, Southern Tibet. Two sediment cores were drilled using a hydraulic borer in Terrace 1 of Lake Chen Co. AMS C-14 dating of the sediments showed that the sequence spanned > 30,000 years. Analyses of present lake hydrology indicated that glacier melt water is very important to maintaining the lake level. Sediment variables such as grain size, TOC, TN, C/N, Fe/Mn, CaCO3, and pollen were analyzed. Warm and moderately humid conditions dominated during the interval 30,000-26,500 cal year BP. From 26,500 to 20,000 cal year BP, chemical variables and pollen assemblages indicate a cold/dry environment, and pollen amounts and assemblages suggest a decline in vegetation. From 20,000 to 18,000 cal year BP, the environment shifted from cold/dry to warm/humid and vegetation rebounded. The environment transitioned to cold/humid during 16,500-10,500 cal year BP, with a cold/dry event around 14,500 cal year BP. After 10,500 cal year BP, the environment in this region tended to be warm/dry, but exhibited three stages. From 10,500 to 9,000 cal year BP, there was a short warm/humid period, but a shift to cold/dry conditions occurred around 9,000 cal year BP. Thereafter, from 9,000 to 6,000 cal year BP, there was a change from cold/dry to warm/humid conditions, with the warmest period around 6,000 cal year BP. After 6,000 cal year BP, the environment cooled rapidly, but then displayed a warming trend. Chemical variables indicate that a relatively warm/dry event occurred around 5,500-5,000 cal year BP, which is supported by time-lagged pollen assemblages around 4,800 cal year BP. Our lake sediment sequence exhibits environmental changes since 30,000 cal year BP, and most features agree with records from the Greenland GISP2 ice core and with other sequences from the Tibetan Plateau. This indicates that environmental changes inferred from Lake Chen Co, Southern Tibet were globally significant.
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