| 1. |
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| 2. |
- Beal, Jacob, et al.
(författare)
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Robust estimation of bacterial cell count from optical density
- 2020
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Ingår i: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
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Tidskriftsartikel (refereegranskat)abstract
- Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
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| 3. |
- Klionsky, Daniel J., et al.
(författare)
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Guidelines for the use and interpretation of assays for monitoring autophagy
- 2012
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Ingår i: Autophagy. - : Informa UK Limited. - 1554-8635 .- 1554-8627. ; 8:4, s. 445-544
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Forskningsöversikt (refereegranskat)abstract
- In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process vs. those that measure flux through the autophagy pathway (i.e., the complete process); thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from stimuli that result in increased autophagic activity, defined as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (in most higher eukaryotes and some protists such as Dictyostelium) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the field understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field.
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| 4. |
- Kristanl, Matej, et al.
(författare)
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The Seventh Visual Object Tracking VOT2019 Challenge Results
- 2019
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Ingår i: 2019 IEEE/CVF INTERNATIONAL CONFERENCE ON COMPUTER VISION WORKSHOPS (ICCVW). - : IEEE COMPUTER SOC. - 9781728150239 ; , s. 2206-2241
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Konferensbidrag (refereegranskat)abstract
- The Visual Object Tracking challenge VOT2019 is the seventh annual tracker benchmarking activity organized by the VOT initiative. Results of 81 trackers are presented; many are state-of-the-art trackers published at major computer vision conferences or in journals in the recent years. The evaluation included the standard VOT and other popular methodologies for short-term tracking analysis as well as the standard VOT methodology for long-term tracking analysis. The VOT2019 challenge was composed of five challenges focusing on different tracking domains: (i) VOT-ST2019 challenge focused on short-term tracking in RGB, (ii) VOT-RT2019 challenge focused on "real-time" short-term tracking in RGB, (iii) VOT-LT2019 focused on long-term tracking namely coping with target disappearance and reappearance. Two new challenges have been introduced: (iv) VOT-RGBT2019 challenge focused on short-term tracking in RGB and thermal imagery and (v) VOT-RGBD2019 challenge focused on long-term tracking in RGB and depth imagery. The VOT-ST2019, VOT-RT2019 and VOT-LT2019 datasets were refreshed while new datasets were introduced for VOT-RGBT2019 and VOT-RGBD2019. The VOT toolkit has been updated to support both standard short-term, long-term tracking and tracking with multi-channel imagery. Performance of the tested trackers typically by far exceeds standard baselines. The source code for most of the trackers is publicly available from the VOT page. The dataset, the evaluation kit and the results are publicly available at the challenge website(1).
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| 5. |
- Kristan, Matej, et al.
(författare)
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The first visual object tracking segmentation VOTS2023 challenge results
- 2023
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Ingår i: 2023 IEEE/CVF International conference on computer vision workshops (ICCVW). - : Institute of Electrical and Electronics Engineers Inc.. - 9798350307443 - 9798350307450 ; , s. 1788-1810
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Konferensbidrag (refereegranskat)abstract
- The Visual Object Tracking Segmentation VOTS2023 challenge is the eleventh annual tracker benchmarking activity of the VOT initiative. This challenge is the first to merge short-term and long-term as well as single-target and multiple-target tracking with segmentation masks as the only target location specification. A new dataset was created; the ground truth has been withheld to prevent overfitting. New performance measures and evaluation protocols have been created along with a new toolkit and an evaluation server. Results of the presented 47 trackers indicate that modern tracking frameworks are well-suited to deal with convergence of short-term and long-term tracking and that multiple and single target tracking can be considered a single problem. A leaderboard, with participating trackers details, the source code, the datasets, and the evaluation kit are publicly available at the challenge website1
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| 6. |
- Kristan, Matej, et al.
(författare)
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The Visual Object Tracking VOT2015 challenge results
- 2015
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Ingår i: Proceedings 2015 IEEE International Conference on Computer Vision Workshops ICCVW 2015. - : IEEE. - 9780769557205 ; , s. 564-586
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Konferensbidrag (refereegranskat)abstract
- The Visual Object Tracking challenge 2015, VOT2015, aims at comparing short-term single-object visual trackers that do not apply pre-learned models of object appearance. Results of 62 trackers are presented. The number of tested trackers makes VOT 2015 the largest benchmark on short-term tracking to date. For each participating tracker, a short description is provided in the appendix. Features of the VOT2015 challenge that go beyond its VOT2014 predecessor are: (i) a new VOT2015 dataset twice as large as in VOT2014 with full annotation of targets by rotated bounding boxes and per-frame attribute, (ii) extensions of the VOT2014 evaluation methodology by introduction of a new performance measure. The dataset, the evaluation kit as well as the results are publicly available at the challenge website(1).
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| 7. |
- Kristan, Matej, et al.
(författare)
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The Visual Object Tracking VOT2016 Challenge Results
- 2016
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Ingår i: COMPUTER VISION - ECCV 2016 WORKSHOPS, PT II. - Cham : SPRINGER INT PUBLISHING AG. - 9783319488813 - 9783319488806 ; , s. 777-823
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Konferensbidrag (refereegranskat)abstract
- The Visual Object Tracking challenge VOT2016 aims at comparing short-term single-object visual trackers that do not apply pre-learned models of object appearance. Results of 70 trackers are presented, with a large number of trackers being published at major computer vision conferences and journals in the recent years. The number of tested state-of-the-art trackers makes the VOT 2016 the largest and most challenging benchmark on short-term tracking to date. For each participating tracker, a short description is provided in the Appendix. The VOT2016 goes beyond its predecessors by (i) introducing a new semi-automatic ground truth bounding box annotation methodology and (ii) extending the evaluation system with the no-reset experiment.
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| 8. |
- Wang, Fang, et al.
(författare)
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Emerging contaminants: A One Health perspective
- 2024
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Ingår i: Innovation. - 2666-6758. ; 5
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Forskningsöversikt (refereegranskat)abstract
- Environmental pollution is escalating due to rapid global development that often prioritizes human needs over planetary health. Despite global efforts to mitigate legacy pollutants, the continuous introduction of new substances remains a major threat to both people and the planet. In response, global initiatives are focusing on risk assessment and regulation of emerging contaminants, as demonstrated by the ongoing efforts to establish the UN's Intergovernmental Science-Policy Panel on Chemicals, Waste, and Pollution Prevention. This review identifies the sources and impacts of emerging contaminants on planetary health, emphasizing the importance of adopting a One Health approach. Strategies for monitoring and addressing these pollutants are discussed, underscoring the need for robust and socially equitable environmental policies at both regional and international levels. Urgent actions are needed to transition toward sustainable pollution management practices to safeguard our planet for future generations.
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| 9. |
- Fan, Weiwei, et al.
(författare)
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Characterizations of a Food Decapeptide Chelating with Zn(II)
- 2020
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Ingår i: EFOOD. - : John Wiley & Sons. - 2666-3066. ; 1:4, s. 326-331
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Tidskriftsartikel (refereegranskat)abstract
- Walnut proteins and peptides have been reported to have great zinc-carrying activity. In this study, the decapeptide EPNGLLLPQY (WP-10) derived from walnut protein has been prepared to figure out zinc binding mechanisms and structure-activity relationships. The space-conformation of this peptide has converted after being chelated with Zn(II). As to secondary structure, the beta-sheet structure of the peptide turned into alpha-helix and beta-turn structure. According to the atomic absorption spectra analysis, one peptide could bind with one Zn(II) via four different binding sites. The signals of different groups in infrared spectroscopy have shifted before and after chelation, and the major functional groups of this peptide involved in chelation are -NH2 and -C=O. GLU1, PRO2, ASP3, GLU4, and LEU6, and the molecular docking proved their participation in the chelating with Zn(II). (c) 2020 International Association of Dietetic Nutrition and Safety. Publishing services by Atlantis Press International B.V. This is an open access article distributed under the CC BY-NC 4.0 license (http://creativecommons.org/licenses/by-nc/4.0/).
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| 10. |
- Jarvis, Erich D., et al.
(författare)
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Whole-genome analyses resolve early branches in the tree of life of modern birds
- 2014
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Ingår i: Science. - : American Association for the Advancement of Science (AAAS). - 0036-8075 .- 1095-9203. ; 346:6215, s. 1320-1331
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Tidskriftsartikel (refereegranskat)abstract
- To better determine the history of modern birds, we performed a genome-scale phylogenetic analysis of 48 species representing all orders of Neoaves using phylogenomic methods created to handle genome-scale data. We recovered a highly resolved tree that confirms previously controversial sister or close relationships. We identified the first divergence in Neoaves, two groups we named Passerea and Columbea, representing independent lineages of diverse and convergently evolved land and water bird species. Among Passerea, we infer the common ancestor of core landbirds to have been an apex predator and confirm independent gains of vocal learning. Among Columbea, we identify pigeons and flamingoes as belonging to sister clades. Even with whole genomes, some of the earliest branches in Neoaves proved challenging to resolve, which was best explained by massive protein-coding sequence convergence and high levels of incomplete lineage sorting that occurred during a rapid radiation after the Cretaceous-Paleogene mass extinction event about 66 million years ago.
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| 11. |
- Li, Xin, et al.
(författare)
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Halide Double Perovskite Nanocrystals Doped with Rare-Earth Ions for Multifunctional Applications
- 2023
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Ingår i: Advanced Science. - 2198-3844. ; 10:20
-
Tidskriftsartikel (refereegranskat)abstract
- Most lead-free halide double perovskite materials display low photoluminescence quantum yield (PLQY) due to the indirect bandgap or forbidden transition. Doping is an effective strategy to tailor the optical properties of materials. Herein, efficient blue-emitting Sb3+-doped Cs2NaInCl6 nanocrystals (NCs) are selected as host, rare-earth (RE) ions (Sm3+, Eu3+, Tb3+, and Dy3+) are incorporated into the host, and excellent PLQY of 80.1% is obtained. Femtosecond transient absorption measurement found that RE ions not only served as the activator ions but also filled the deep vacancy defects. Anti-counterfeiting, optical thermometry, and white-light-emitting diodes (WLEDs) are exhibited using these RE ions-doped halide double perovskite NCs. For the optical thermometry based on Sm3+-doped Cs2NaInCl6:Sb3+ NCs, the maximum relative sensitivity is 0.753% K−1, which is higher than those of most temperature-sensing materials. Moreover, the WLED fabricated by Sm3+-doped Cs2NaInCl6:Sb3+ NCs@PMMA displays CIE color coordinates of (0.30, 0.28), a luminous efficiency of 37.5 lm W−1, a CCT of 8035 K, and a CRI over 80, which indicate that Sm3+-doped Cs2NaInCl6:Sb3+ NCs are promising single-component white-light-emitting phosphors for next-generation lighting and display technologies.
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| 12. |
- Ning, Rui, et al.
(författare)
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Assessing progression limits in different grades of keratoconus from a novel perspective : precision of measurements of the corneal epithelium
- 2024
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Ingår i: Eye and Vision. - 2326-0254. ; 11:1
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Tidskriftsartikel (refereegranskat)abstract
- Background: To assess repeatability and reproducibility of corneal epithelium thickness (ET) measured by a spectral-domain optical coherence tomographer (SD-OCT)/Placido topographer (MS-39, CSO, Florence, Italy) in keratoconus (KC) population at different stages, as well as to determine the progression limits for evaluating KC progression. Methods: A total of 149 eyes were enrolled in this study, with 29 eyes in the forme fruste keratoconus (FFKC) group, 34 eyes in the mild KC group, 40 eyes in the moderate KC group, and 46 eyes in the severe KC group. Employing the within-subject standard deviation (Sw), test-retest variability (TRT), coefficient of variation (CoV), and intraclass correlation coefficient (ICC) to evaluate intraoperator repeatability and interoperator reproducibility. Results: The repeatability and reproducibility of MS-39 in patients with KC were acceptable, according to ICC values ranging from 0.732 to 0.954. However, patients with more severe KC and progressive peripheralization of the measurement points had higher TRTs but a thinning trend. The current study tended to set the cut-off values of mild KC, moderate KC, and severe KC to 4.9 µm, 5.2 µm, and 7.4 µm for thinnest epithelium thickness (TET). When differences between follow-ups are higher than those values, progression of the disease is possible. As for center epithelium thickness (CET), cut-off values for mild KC, moderate KC, and severe KC should be 2.8 µm, 4.4 µm, and 5.3 µm. This might be useful in the follow-up and diagnosis of keratoconus. Conclusions: This study demonstrated that the precision of MS-39 was reduced in measuring more severe KC patients and more peripheral corneal points. In determining disease progression, values should be differentiated between disease-related real changes and measurement inaccuracies. Due to the large difference in ET measured by MS-39 between various stages of disease progression, it is necessary to accurately grade KC patients to avoid errors in KC clinical decision-making.
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| 13. |
- Zhang, Shucan, et al.
(författare)
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Temperature induced superhard CrB2 coatings with preferred (001) orientation deposited by DC magnetron sputtering technique
- 2017
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Ingår i: Surface & Coatings Technology. - : ELSEVIER SCIENCE SA. - 0257-8972 .- 1879-3347. ; 322, s. 134-140
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Tidskriftsartikel (refereegranskat)abstract
- The influence of deposition temperature in the range of 100 degrees C to 400 degrees C on the microstructure and mechanical properties of CrB2 coatings by DC magnetron sputtering was studied. The coating texture changed from random mixed orientation with (101) and (001) planes to the preferred (001) orientation when increasing the deposition temperature. Moreover, the microstructure coating evolved from an underdense structure to a bulky columnar structure (similar to 50 nm), and finally to a dense nanoscale columnar structure (similar to 7 nm). This structural densification was mainly attributed to the enhanced atomic surface diffusion with increasing deposition temperature. It resulted in promotion of the (001) preferred orientation and greatly enhanced the mechanical properties. Specifically, when the deposition temperature was 300 degrees C, the CrB2 coatings exhibited the highest toughness while superhardness (51 +/- 2 GPa) was achieved for coating grown at 400 degrees C. (C) 2017 Published by Elsevier B.V.
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| 14. |
- Asayama, Kei, et al.
(författare)
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Setting Thresholds to Varying Blood Pressure Monitoring Intervals Differentially Affects Risk Estimates Associated With White-Coat and Masked Hypertension in the Population
- 2014
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Ingår i: Hypertension. - 0194-911X .- 1524-4563. ; 64:5, s. 935-942
-
Tidskriftsartikel (refereegranskat)abstract
- Outcome-driven recommendations about time intervals during which ambulatory blood pressure should be measured to diagnose white-coat or masked hypertension are lacking. We cross-classified 8237 untreated participants (mean age, 50.7 years; 48.4% women) enrolled in 12 population studies, using >= 140/>= 90, >= 130/>= 80, >= 135/>= 85, and >= 120/>= 70 mm Hg as hypertension thresholds for conventional, 24-hour, daytime, and nighttime blood pressure. White-coat hypertension was hypertension on conventional measurement with ambulatory normotension, the opposite condition being masked hypertension. Intervals used for classification of participants were daytime, nighttime, and 24 hours, first considered separately, and next combined as 24 hours plus daytime or plus nighttime, or plus both. Depending on time intervals chosen, white-coat and masked hypertension frequencies ranged from 6.3% to 12.5% and from 9.7% to 19.6%, respectively. During 91 046 person-years, 729 participants experienced a cardiovascular event. In multivariable analyses with normotension during all intervals of the day as reference, hazard ratios associated with white-coat hypertension progressively weakened considering daytime only (1.38; P=0.033), nighttime only (1.43; P=0.0074), 24 hours only (1.21; P=0.20), 24 hours plus daytime (1.24; P=0.18), 24 hours plus nighttime (1.15; P=0.39), and 24 hours plus daytime and nighttime (1.16; P=0.41). The hazard ratios comparing masked hypertension with normotension were all significant (P<0.0001), ranging from 1.76 to 2.03. In conclusion, identification of truly low-risk white-coat hypertension requires setting thresholds simultaneously to 24 hours, daytime, and nighttime blood pressure. Although any time interval suffices to diagnose masked hypertension, as proposed in current guidelines, full 24-hour recordings remain standard in clinical practice.
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| 15. |
- Chen, Leilei, et al.
(författare)
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High-temperature wear mechanisms of TiNbWN films : Role of nanocrystalline oxides formation
- 2023
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Ingår i: Friction. - : Springer. - 2223-7690 .- 2223-7704. ; 10
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Tidskriftsartikel (refereegranskat)abstract
- Refractory high/medium entropy nitrides (HENs/MENs) exhibit comprehensive application prospects as protective films on mechanical parts, particularly those subjected to sliding contacts at elevated temperatures. In this study, a new MEN system TiNbWN, forming a single fcc solution, is designed and its wear performance at temperatures ranging from 25 to 750 °C is explored. The wear mechanisms can be rationalized by examining the subsurface microstructural evolutions using the transmission electron microscopy as well as calculating the phase diagrams and interfacial adhesion behavior employing calculation of phase diagram (CALPHAD) and density functional theory (DFT). To be specific, increased wear losses occur in a temperature range of 25–600 °C, being predominantly caused by the thermally-induced hardness degradation; whereas at the ultimate temperature (750 °C), the wear loss is refrained due to the formation of nanocrystalline oxides (WnO3n−2, TiO2, and γTiOx), as synergistically revealed by microscopy and CALPHAD, which not only enhance the mechanical properties of the pristine nitride film, but also act as solid lubricants, reducing the interfacial adhesion. Thus, our work delineates the role of the in situ formed nanocrystalline oxides in the wear mechanism transition of TiNbWN thin films, which could shed light on the high-temperature wear behavior of refractory HEN/MEN films.
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| 16. |
- Fang, Wang, et al.
(författare)
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Green recovery of cropland carrying capacity in developed regions : empirical evidence from Guangdong, China
- 2023
-
Ingår i: Economic Change and Restructuring. - 1573-9414 .- 1574-0277. ; 56:3, s. 2405-2436
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Tidskriftsartikel (refereegranskat)abstract
- This paper evaluates the carrying capacity of cultivated land in Guangdong Province,China, using the entropy weight method. Ecological and environmental pressuresignificantly impacts capacity, while economic and social factors are stable.Production pressure fluctuates and rises. To improve capacity, we must reduce ecologicaland environmental pressure, protect cultivated land resources, develop andpromote green technology, and maintain water conservation facilities. The resultsindicate that reducing ecological and environmental pressure is essential to improvethe carrying capacity of cultivated land in Guangdong Province. In conclusion, thisstudy highlights the importance of balancing economic growth with environmentalsustainability in developing regions like Guangdong Province. It suggests that aholistic approach that considers ecological, economic, and social factors is necessaryto ensure long-term food security and sustainable land use practices.
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| 17. |
- Franklin, Stanley S., et al.
(författare)
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The Cardiovascular Risk of White-Coat Hypertension
- 2016
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Ingår i: Journal of the American College of Cardiology. - : Elsevier BV. - 0735-1097 .- 1558-3597. ; 68:19, s. 2033-2043
-
Tidskriftsartikel (refereegranskat)abstract
- BACKGROUND The role of white-coat hypertension (WCH) and the white-coat-effect (WCE) in development of cardiovascular disease (CVD) risk remains poorly understood. OBJECTIVES Using data from the population-based, 11-cohort IDACO (International Database on Ambulatory Blood Pressure Monitoring in Relation to Cardiovascular Outcomes), this study compared daytime ambulatory blood pressure monitoring with conventional blood pressure measurements in 653 untreated subjects with WCH and 653 normotensive control subjects. METHODS European Society Hypertension guidelines were used as a 5-stage risk score. Low risk was defined as 0 to 2 risk factors, and high risk was defined as >= 3 to 5 risk factors, diabetes, and/or history of prior CVD events. Age-and cohort-matching was done between 653 untreated subjects with WCH and 653 normotensive control subjects. RESULTS In a stepwise linear regression model, systolic WCE increased by 3.8 mm Hg (95% confidence interval [CI]: 3.1 to 4.6 mm Hg) per 10-year increase in age, and was similar in low-and high-risk subjects with or without prior CVD events. Over a median 10.6-year follow-up, incidence of new CVD events was higher in 159 high-risk subjects with WCH compared with 159 cohort-and age-matched high-risk normotensive subjects (adjusted hazard ratio [HR]: 2.06; 95% CI: 1.10 to 3.84; p = 0.023). The HR was not significant for 494 participants with low-risk WCH and age-matched low-risk normotensive subjects. Subgroup analysis by age showed that an association between WCH and incident CVD events is limited to older (age >= 60 years) high-risk WCH subjects; the adjusted HR was 2.19 (95% CI: 1.09 to 4.37; p = 0.027) in the older high-risk group and 0.88 (95% CI: 0.51 to 1.53; p = 0.66) in the older low-risk group (p for interaction = 0.044). CONCLUSIONS WCE size is related to aging, not to CVD risk. CVD risk in most persons with WCH is comparable to age-and risk-adjusted normotensive control subjects.
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| 18. |
- Han, Ning, et al.
(författare)
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Achieving Efficient Electrocatalytic Oxygen Evolution in Acidic Media on Yttrium Ruthenate Pyrochlore through Cobalt Incorporation
- 2023
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Ingår i: Advanced Functional Materials. - : Wiley. - 1616-301X .- 1616-3028. ; 33:20
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Tidskriftsartikel (refereegranskat)abstract
- The development of electrocatalysts for the oxygen evolution reaction (OER) especially in acidic media remains the major challenge that still requires significant advances, both in material design and mechanistic exploration. In this study, the incorporation of cobalt in Y2-xCoxRu2O7−δ results in an ultrahigh OER activity because of the charge redistribution at eg orbitals between Ru and Co atoms. The Y1.75Co0.25Ru2O7−δ electrocatalyst exhibits an extremely small overpotential of 275 mV in 0.5 m H2SO4 at the current density of 10 mA cm−2, which is smaller than that of parent Y2Ru2O7−δ (360 mV) and commercial RuO2 (286 mV) catalysts. The systematic investigation of the composition related to OER activity shows that the Co substitution will also bring other effective changes, such as reducing the bandgap, and creating oxygen vacancies, which result in fast OER charge transfer. Meanwhile, the strengthening of the bond hybridization between the d orbitals of metal (Y and Ru) and the 2p orbitals of O will intrinsically enhance the chemical stability. Finally, theoretical calculations indicate that cobalt substitution reduces the theoretical overpotential both through an adsorbate evolution mechanism and a lattice oxygen-mediated mechanism.
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| 19. |
- Klionsky, Daniel J., et al.
(författare)
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Guidelines for the use and interpretation of assays for monitoring autophagy in higher eukaryotes
- 2008
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Ingår i: Autophagy. - : Landes Bioscience. - 1554-8627 .- 1554-8635. ; 4:2, s. 151-175
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Forskningsöversikt (refereegranskat)abstract
- Research in autophagy continues to accelerate,1 and as a result many new scientists are entering the field. Accordingly, it is important to establish a standard set of criteria for monitoring macroautophagy in different organisms. Recent reviews have described the range of assays that have been used for this purpose.2,3 There are many useful and convenient methods that can be used to monitor macroautophagy in yeast, but relatively few in other model systems, and there is much confusion regarding acceptable methods to measure macroautophagy in higher eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers of autophagosomes versus those that measure flux through the autophagy pathway; thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from fully functional autophagy that includes delivery to, and degradation within, lysosomes (in most higher eukaryotes) or the vacuole (in plants and fungi). Here, we present a set of guidelines for the selection and interpretation of the methods that can be used by investigators who are attempting to examine macroautophagy and related processes, as well as by reviewers who need to provide realistic and reasonable critiques of papers that investigate these processes. This set of guidelines is not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to verify an autophagic response.
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| 20. |
- Li, Han, et al.
(författare)
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Advancements of nature nanocage protein : preparation, identification and multiple applications of ferritins
- 2022
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Ingår i: Critical reviews in food science and nutrition. - : Informa UK Limited. - 1040-8398 .- 1549-7852. ; 62:25, s. 7117-7128
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Forskningsöversikt (refereegranskat)abstract
- Ferritin is an important iron storage protein, which is widely existed in all forms of life. Ferritin can regulate iron homeostasis when iron ions are lacking or enriched in the body, so as to avoid iron deficiency diseases and iron poisoning. Ferritin presents a hollow nanocage, which can store ions or other small molecular substances in the cavity. Therefore, ferritin shows its potential as a functional nanomaterial that can deliver nutrients or drugs in a targeted manner to improve bioavailability. Due to the special structure, the research on ferritin has attracted more and more attention in recent years. In this paper, the structural characteristics of ferritin were introduced, and the natural purification and prokaryotic expression methods of ferritin from different sources were described. At the same time, ferritin can bind to small molecules, so that it has the activity of small molecules, to construct a new type of ferritin. As a result, ferritin plays an important role as a nutrient substance, in targeted transport, and disease monitoring, etc. In conclusion, the yield of ferritin can be improved by means of molecular biology. Meanwhile, molecular modification can be used to make ferritin have unique activity and function, which lays a foundation for subsequent research.
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| 21. |
- Li, Han, et al.
(författare)
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Improvement of thermal stability of oyster (Crassostrea gigas) ferritin by point mutation
- 2021
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Ingår i: Food Chemistry. - : Elsevier. - 0308-8146 .- 1873-7072. ; 346
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Tidskriftsartikel (refereegranskat)abstract
- Ferritin can be widely used as functional nanomaterial. But the physiological activity of ferritin can be damaged under excessive temperatures, which affect the self-assembly property. In this study, point mutation was produced in Asp120 to Gly120 of ferritin amino acid sequence and the heat resistance was improved significantly. The thermal denaturation temperature of mutated ferritin is 89.17 degrees C and has increased by 13 degrees C more than the wildtype oyster ferritin. The effect of thermal treatment on the denaturation, aggregation state, particle size and the structure of ferritin was not changed before 90 degrees C. The computational modeling and analysis indicated that mutated ferritin promotes the overall structural stability assembly via decreasing the interaction energies of 62 percent energies in 3-fold interface. Improving the thermal stability of oyster ferritin by point mutation enhances its applications as a food ingredient.
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| 22. |
- Sun, Xiaoyan, et al.
(författare)
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Association of neurogranin gene expression with Alzheimer's disease pathology in the perirhinal cortex.
- 2021
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Ingår i: Alzheimer's & dementia (New York, N. Y.). - : Wiley. - 2352-8737. ; 7:1
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Tidskriftsartikel (refereegranskat)abstract
- Synaptic damage is a key pathology of Alzheimer's disease (AD). The mechanism underlying synaptic vulnerability in AD remains elusive.Using a large-scale transcriptomic dataset, we analyzed the neurogranin-centered integrative gene network and assessed the correlation of neurogranin (NRGN) gene expression with AD pathology in post mortem brains. We studied the association of NRGN expression with Clinical Dementia Rating (CDR) and neuropathological diagnosis of AD.We find that the genes positively correlated with NRGN expression in AD are involved in synaptic transmission and cation channel pathways. NRGN expression is correlated with amyloid and tau pathology in the perirhinal cortex of post mortem brains. NRGN expression is associated with the diagnosis of AD and correlated with CDR.Transcriptional regulation of the gene encoding for synaptic protein is involved in selective synaptic damage in AD. Identifying the genes associated with synaptic damage pathways in AD may provide targets for intervention.
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| 23. |
- Wang, Chunyu, et al.
(författare)
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Co-regulation of temperature and moisture in the irrigated agricultural ecosystem productivity
- 2023
-
Ingår i: Agricultural Water Management. - : Elsevier BV. - 0378-3774. ; 275
-
Tidskriftsartikel (refereegranskat)abstract
- Agroecosystem photosynthesis is key to coping with global climate change. In farmland where human activities are highly involved, the interaction between environmental factors and their influences on gross primary productivity (GPP) are insufficiently understood. Particularly, the irrigation and mulching in water-saving agriculture can alter the crop responses to environmental change. Based on eddy covariance measurements of maize fields under mulched drip irrigation (DM) and mulched border irrigation (BM) in arid areas of Northwest China from 2014 to 2018, we systematically studied the interaction between multiple environmental factors and their independent effects on GPP using structural equation modeling, partial correlation coefficient and decoupling analysis by bins. The top three factors exerting the largest total effects on the GPP were soil temperature (Ts), canopy temperature (Tc) and vapor pressure deficit (VPD), among which Ts (0.75) and Tc (0.66) had the largest total effect on GPP under DM and BM, respectively. The independent effects of Ts, soil water content (SWC) and VPD on GPP were different under the two irrigation methods. SWC after excluding the influence of Ts showed a negative effect on GPP under DM (−1.24 g Cm−2d−1), while a positive effect under BM (0.02 g Cm−2d−1). By contrast, SWC after excluding the influence of VPD showed a positive effect on GPP under DM (0.59 g Cm−2d−1), while a negative effect under BM (−0.05 g Cm−2d−1). Interestingly, higher Ts, lower SWC and higher VPD had the potential to increase GPP under the two irrigation methods. We also found that the total effects of irrigation and VPD as well as the indirect effects of environmental factors on GPP should not be ignored. Our study will provide important reference for dealing with the effect of high temperature and drought stress on agro-ecosystem GPP and evaluating the response of vegetation to environmental factors.
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| 24. |
- Wang, Chunyu, et al.
(författare)
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Water use efficiency control for a maize field under mulched drip irrigation
- 2023
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Ingår i: Science of the Total Environment. - : Elsevier BV. - 0048-9697. ; 857
-
Tidskriftsartikel (refereegranskat)abstract
- Agricultural ecosystem water use efficiency (WUE) is an important indicator reflecting carbon-water coupling, but its control mechanisms in managed fields remain unclear. In order to reveal the influencing factors of WUE in the agricultural field under mulched drip irrigation (DM), we carried out the 8-year continuous observations in a maize field from Northwestern China. The structural equation model, relative importance analysis and principal component analysis were used to quantify the regulation effects of environmental and biological factors on WUE at different time scales, in different growth stages and under different hydrothermal conditions. The results showed that annual WUE varied between 2.18 g C Kg−1 H2O and 3.60 g C Kg−1 H2O, with a multi-year mean of 2.91 g C Kg−1 H2O. The total effects of air temperature on the daily WUE in the whole growth period, the vegetative growth stage, the warm and dry years, the cold and wet years, and the warm and wet years were the largest, with values of 0.61, 0.80, 0.70, 0.70 and 0.91 respectively. However, vapor pressure deficit and net radiation had the largest total effect in the cold and dry years (−0.63) and the reproductive growth stage (−0.49), respectively. Leaf biomass played a leading role in regulating the daily and interannual WUE, and the relative importance of leaf biomass to WUE in the vegetative growth stage was up to 75 %. In the warm and wet years, the relative importance of root biomass to WUE was 33 %, slightly higher than that of leaf biomass (31 %). At the same time, we found that Ta has the potential to increase WUE under future climate warming. Our results improve the understanding of carbon-water coupling mechanisms and provide important enlightenment on how crop ecosystems should adapt to future climate change.
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| 25. |
- Wang, Jiechen, et al.
(författare)
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A parallel algorithm for constructing Voronoi diagrams based on point-set adaptive grouping
- 2014
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Ingår i: Concurrency and Computation. - : Wiley. - 1532-0626 .- 1532-0634. ; 26:2, s. 434-446
-
Tidskriftsartikel (refereegranskat)abstract
- This paper presents a parallel algorithm for constructing Voronoi diagrams based on point-set adaptive grouping. The binary tree splitting method is used to adaptively group the point set in the plane and construct sub-Voronoi diagrams for each group. Given that the construction of Voronoi diagrams in each group consumes the majority of time and that construction within one group does not affect that in other groups, the use of a parallel algorithm is suitable.After constructing the sub-Voronoi diagrams, we extracted the boundary points of the four sides of each sub-group and used to construct boundary site Voronoi diagrams. Finally, the sub-Voronoi diagrams containing each boundary point are merged with the corresponding boundary site Voronoi diagrams. This produces the desired Voronoi diagram. Experiments demonstrate the efficiency of this parallel algorithm, and its time complexity is calculated as a function of the size of the point set, the number of processors, the average number of points in each block, and the number of boundary points.
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| 26. |
- Wang, Na, et al.
(författare)
-
Label-free analysis of the β-hydroxybutyricacid drug on mitochondrial redox states repairment in type 2 diabetic mice by resonance raman scattering
- 2024
-
Ingår i: Biomedicine and Pharmacotherapy. - : Elsevier BV. - 0753-3322 .- 1950-6007. ; 172
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Tidskriftsartikel (refereegranskat)abstract
- Background: Mitochondrial redox imbalance underlies the pathophysiology of type2 diabetes mellitus (T2DM), and is closely related to tissue damage and dysfunction. Studies have shown the beneficial effects of dietary strategies that elevate β-hydroxybutyrate (BHB) levels in alleviating T2DM. Nevertheless, the role of BHB has not been clearly elucidated. Methods: We performed a spectral study to visualize the preventive effects of BHB on blood and multiorgan mitochondrial redox imbalance in T2DM mice via using label-free resonance Raman spectroscopy (RRS), and further explored the impact of BHB therapy on the pathology of T2DM mice by histological and biochemical analyses. Findings: Our data revealed that RRS-based mitochondrial redox states assay enabled clear and reliable identification of the improvement of mitochondrial redox imbalance by BHB, evidenced by the reduction of Raman peak intensity at 750 cm−1, 1128 cm−1 and 1585 cm−1 in blood, tissue as well as purified mitochondria of db/db mice and the increase of tissue mitochondrial succinic dehydrogenase (SDH) staining after BHB treatment. Exogenous supplementation of BHB was also found to attenuate T2DM pathology related to mitochondrial redox states, involving organ injury, blood glucose control, insulin resistance and systemic inflammation. Interpretation: Our findings provide strong evidence for BHB as a potential therapeutic strategy targeting mitochondria for T2DM.
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| 27. |
- Wang, Wei, et al.
(författare)
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Ag@SiO2 Core-Shell Nanoparticles for Probing Spatial Distribution of Electromagnetic Field Enhancement via Surface-Enhanced Raman Scattering
- 2009
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Ingår i: ACS Nano. - : American Chemical Society (ACS). - 1936-086X .- 1936-0851. ; 3:11, s. 3493-3496
-
Tidskriftsartikel (refereegranskat)abstract
- We show that the spatial distribution of the electromagnetic (EM) field enhancement can be probed directly via dynamic evolution of surface-enhanced Raman scattering (SERS) of rhodamine 6G (R6G) molecules as they diffuse into Ag@SiO2 core-shell nanoparticles. The porous silica shell limits the diffusion of R6G molecules toward Inner Ag cores, thereby allowing direct observation and quantification of the spatial distribution of SERS enhancement as molecules migrate from the low to high EM fields inside the dielectric silica shell. Our experimental evidence is validated by the generalized Mie theory, and the approach can potentially offer a novel platform for further investigating the site and spatial distribution of the EM fields and the EM versus chemical enhancement of SERS due to molecular confinement within the Ag@SiO2 nanoshell.
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| 28. |
- Wang, Zhiqi, et al.
(författare)
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Topology Optimization of Passive Cell Traps
- 2021
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Ingår i: Micromachines. - : MDPI. - 2072-666X. ; 12:7
-
Tidskriftsartikel (refereegranskat)abstract
- This paper discusses a flexible design method of cell traps based on the topology optimization of fluidic flows. Being different from the traditional method, this method obtains the periodic layout of the cell traps according to the cell trapping requirements by proposing a topology optimization model. Additionally, it satisfies the cell trapping function by restricting the flow distribution while taking into account the overall energy dissipation of the flow field. The dependence on the experience of the designer is reduced when this method is used to design a cell trap with acceptable trapping performance. By comparing the influence of the changes of various parameters on the optimization results, the flexibility of the topology optimization method for cell trap structure optimization is verified. The capability of this design method is validated by several performed comparisons between the obtained layouts and optimized designs in the published literature.
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| 29. |
- Wu, Di, et al.
(författare)
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Biological and conventional food processing modifications on food proteins : Structure, functionality, and bioactivity
- 2020
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Ingår i: Biotechnology Advances. - : Elsevier BV. - 0734-9750 .- 1873-1899. ; 40
-
Forskningsöversikt (refereegranskat)abstract
- Food proteins are important nutrients for human health and thus make significant contributions to the unique functions of different foods. The modification of proteins through physical and biological processing could improve the functional and nutritional properties of food products; these changes can be attributed to modifications in particle size, solubility, emulsion stability, secondary structure, as well as the bioactivities of the proteins. Physical processing treatments might promote physical phenomena, such as combined friction, collision, shear forces, turbulence, and cavitation of particles, and lead to changes in the particle sizes of proteins. The objective of this review is to illustrate the effect of physical and biological processing on the structure, and physical and chemical properties of food-derived proteins and provide insights into the mechanism underlying structural changes. Many studies have suggested that physical and biological processes, such as ultrasound treatment, high pressure homogenization, ball mill treatment, and enzymatic hydrolysis could affect the structure, physical properties, and chemical properties of food-derived proteins. Some important applications of food-derived proteins are also discussed based on the relationships between their physical, chemical, and functional properties. Perspectives from fundamental or practical research are also brought in to provide a complete picture of the currently available relevant data.
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| 30. |
- Wu, Di, et al.
(författare)
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Coiled-coil scallops (Chlamys farreri) peptide hydrogel with metal ionic and temperature tunable assembly
- 2024
-
Ingår i: Food Chemistry. - : Elsevier. - 0308-8146 .- 1873-7072. ; 433
-
Tidskriftsartikel (refereegranskat)abstract
- Self-assembly of peptides is a powerful method of preparing nanostructured materials. Peptides frequently utilize charged groups as a convenient switch for controlling assembly state by pH, ionic strength or temperature. In this study, the molecular properties and gel-forming ability of Chlamys farreri protein hydrolysates were studied. According to self-assembled theory, the presence of isoleucine at position 'a' and leucine at 'd' causes a switch between coiled-coil structures. Compared to P-2-CG, the components of & alpha;-helix (23.60 & PLUSMN; 0.56%) were changed into 8-sheet (4.83 & PLUSMN; 2.86%) in the secondary structure of the hydrogel induced by ZnCl2. NMR siginals appeared at high field,which indicated hydrogen bonds were formed between P-2-CG and solvent environments at 20 degrees C. With temperature going up, the hydrogen bonds were broken and nanofibrils were changed into dense aggre-gates. We expected that P-2-CG could provide a new candidate for preparing metal-induced nanofibers or hydrogels with further applications in food industry.
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| 31. |
- Xu, Zhe, et al.
(författare)
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Absorption and transport of a Mytilus edulis-derived peptide with the function of preventing osteoporosis
- 2021
-
Ingår i: Food & Function. - : Royal Society of Chemistry. - 2042-6496 .- 2042-650X. ; 12:5, s. 2102-2111
-
Tidskriftsartikel (refereegranskat)abstract
- The YPRKDETGAERT peptide (PME-1) identified from the Mytilus edulis proteins has been shown to promote the proliferation and differentiation of osteoblasts and it has good bone-forming activity in vitro. Further, PME-1 has been shown to prevent osteoporosis in vivo. PME-1 can be absorbed through the gastrointestinal tract, and the passing rate in monolayer Caco-2 cells was 6.57%. PME-1 can also enter the blood circulation and the concentration of PME-1 in serum reached the maximum, 61.06 +/- 26.32 ng mL(-1), 20 min after feeding. The multifunctional in vivo imager was used to further determine the distribution of the 5-FITC-(Acp)-YPRKDETGAERT peptide (PME-1-FITC) 2 h after feeding the peptide, and the result confirmed the above results and showed that a part of PME-1-FITC can affect bone in vivo. Therefore, PME-1 not only was easily absorbed in the gastrointestinal tract, but also has the potential beneficial effect on preventing osteoporosis.
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| 32. |
- Yang, Li, et al.
(författare)
-
Premixed jet flame characteristics of syngas using OH planar laser induced fluorescence
- 2011
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Ingår i: Chinese Science Bulletin. - : Springer Science and Business Media LLC. - 1001-6538 .- 1861-9541. ; 56:26, s. 2862-2868
-
Tidskriftsartikel (refereegranskat)abstract
- Lean premixed flame characteristics of several typical low calorific value (LCV) syngases (basis CO/H-2/CH4/CO2/N-2), including bituminous coal, wood residue, corn core, and wheat straw gasification syngas, were investigated using OH planar laser induced fluorescence (PLIF) technology. OH radical distributions within the turbulent flame were measured for different turbulence intensities. Flame structures of syngases were analyzed and characterized with respect to burnt and unburnt regions, flame curvature (sharp cusp), local extinction (holes and penetration), OH reaction layer thickness, wrinkling, and other features, with OH-PLIF instantaneous images and statistical analysis. Results show that H-2 content, LCV, and turbulence intensity are the most effective factors influencing the OH radical intensity and thickness of OH radical layers. The bituminous coal gasification syngas with relatively higher LCV and H-2 content tends to burn out easily. Through changes in thickness of the OH radical layers and signal intensities, the reaction layer can be compressed by intensifying turbulence and thereby the combustion processes of syngas.
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| 33. |
- Yang, Meilian, et al.
(författare)
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Myosin heavy chain-derived peptide of Gadus morhua promotes proliferation and differentiation in osteoblasts and bone formation and maintains bone homeostasis in ovariectomized mice
- 2023
-
Ingår i: Food & Function. - : Royal Society of Chemistry. - 2042-6496 .- 2042-650X. ; 14:11, s. 5151-5166
-
Tidskriftsartikel (refereegranskat)abstract
- Gadus morhua is an important commercial fish rich in nutrients required for daily metabolism. However, the regulation of G. morhua peptides (GMP) on osteoblast growth remains unclear. In order to clarify the regulatory effects of GMP on osteoblasts, the effects of GMP on the growth of MC3T3-E1 cells were investigated, and the osteogenic peptides were identified and screened. The results showed that GMP promoted the proliferation and differentiation of osteoblasts by regulating the BMP/WNT signaling pathway at concentrations of 1-100 mu g mL(-1). Molecular docking studies showed that a decapeptide, MNKKREAEFQ (P-GM-1), had a high affinity for integrins 3VI4 and 1L5G (-CDOCKER interaction energy: 161.30, 212.27 kcal mol(-1)). Additionally, the proliferation rate of MC3T3-E1 cells was increased by 27%, and ALP activity was significantly increased under P-GM-1 treatment (100 mu g mL(-1)). Moreover, P-GM-1 promotes bone formation, maintains bone homeostasis, and prevents osteoporosis in ovariectomized mice by regulating the BMP/Smad signaling pathway. This study confirmed the potential of GMP in the regulation of bone mineral density and provided a certain theoretical basis for the development of anti-osteoporosis active factors from GMP.
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| 34. |
- Yang, Meilian, et al.
(författare)
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Novel Peptide Derived from Gadus morhua Stimulates Osteoblastic Differentiation and Mineralization through Wnt/β-Catenin and BMP Signaling Pathways
- 2024
-
Ingår i: Journal of Agricultural and Food Chemistry. - : American Chemical Society (ACS). - 0021-8561 .- 1520-5118. ; 72:17, s. 9691-9702
-
Tidskriftsartikel (refereegranskat)abstract
- Marine biodiversity offers a wide array of active ingredient resources. Gadus morhua peptides (GMPs) showed excellent osteoprotective effects in ovariectomized mice. However, the potential osteogenesis mechanisms of key osteogenic peptides in GMP were seldom reported. In this study, a novel osteogenic peptide (GETNPADSKPGSIR, P-GM-2) was screened from GMP. P-GM-2 has a high stability coefficient and a strong interaction with epidermal growth factor receptor. Cell culture experiments showed that P-GM-2 stimulated the expression of osteogenic differentiation markers to promote osteoblast proliferation, differentiation, and mineralization. Additionally, P-GM-2 phosphorylates GSK-3β, leading to the stabilization of β-catenin and its translocation to the nucleus, thus initiating the activation of the Wnt/β-catenin signaling pathway. Meanwhile, P-GM-2 could also regulate the osteogenic differentiation of preosteoblasts by triggering the BMP/Smad and mitogen-activated protein kinase signaling pathways. Further validation with specific inhibitors (ICG001 and Noggin) demonstrated that the osteogenic activity of P-GM-2 was revealed by the activation of the BMP and Wnt/β-catenin pathways. In summary, these results provide theoretical and practical insights into P-GM-2 as an effective antiosteoporosis active ingredient.
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| 35. |
- Zhang, Kaicheng, et al.
(författare)
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SN 2014J in M82 : new insights on the spectral diversity of Type Ia supernovae
- 2018
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Ingår i: Monthly notices of the Royal Astronomical Society. - : Oxford University Press (OUP). - 0035-8711 .- 1365-2966. ; 481:1, s. 878-893
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Tidskriftsartikel (refereegranskat)abstract
- We present extensive spectroscopic observations for one of the closest Type Ia supernovae (SNe Ia), SN 2014J discovered in M82, ranging from 10.4 d before to 473.2 d after B-band maximum light. The diffuse interstellar band features detected in a high-resolution spectrum allow an estimate of line-of-sight extinction as A(v) similar to 1.9 +/- 0.6 mag. Spectroscopically, SN 2014J can be put into the high-velocity (HV) subgroup in Wang's classification with a velocity of Si II lambda 6355 at maximum light of upsilon(0) = 1.22 +/- 0.01 x 10(4) km s(-1) but has a low velocity gradient (LVG, following Benetti's classification) of (v) over bar = 41 +/- 2 km s(-1) d(-1), which is inconsistent with the trend that HV SNe Ia generally have larger velocity gradients. We find that the HV SNe Ia with LVGs tend to have relatively stronger Si III (at similar to 4400 angstrom) absorptions in early spectra, larger ratios of S II lambda 5468 to S II lambda 5640, and weaker Si II 5972 absorptions compared to their counterparts with similar velocities but high velocity gradients. This shows that the HV+ LVG subgroup of SNe Ia may have intrinsically higher photospheric temperature, which indicates that their progenitors may experience more complete burning in the explosions relative to the typical HV SNe Ia.
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| 36. |
- Zhang, Miao, 1985-, et al.
(författare)
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Super-resolved Optical Mapping of Reactive Sulfur-Vacancies in Two-Dimensional Transition Metal Dichalcogenides
- 2021
-
Ingår i: ACS Nano. - : American Chemical Society (ACS). - 1936-0851 .- 1936-086X. ; 15:4, s. 7168-7178
-
Tidskriftsartikel (refereegranskat)abstract
- Transition metal dichalcogenides (TMDs) represent a class of semiconducting two-dimensional (2D) materials with exciting properties. In particular, defects in 2D-TMDs and their molecular interactions with the environment can crucially affect their physical and chemical properties. However, mapping the spatial distribution and chemical reactivity of defects in liquid remains a challenge. Here, we demonstrate large area mapping of reactive sulfur-deficient defects in 2D-TMDs in aqueous solutions by coupling single-molecule localization microscopy with fluorescence labeling using thiol chemistry. Our method, reminiscent of PAINT strategies, relies on the specific binding of fluorescent probes hosting a thiol group to sulfur vacancies, allowing localization of the defects with an uncertainty down to 15 nm. Tuning the distance between the fluorophore and the docking thiol site allows us to control Foster resonance energy transfer (FRET) process and reveal grain boundaries and line defects due to the local irregular lattice structure. We further characterize the binding kinetics over a large range of pH conditions, evidencing the reversible adsorption of the thiol probes to the defects with a subsequent transitioning to irreversible binding in basic conditions. Our methodology provides a simple and fast alternative for large-scale mapping of nonradiative defects in 2D materials and can be used for in situ and spatially resolved monitoring of the interaction between chemical agents and defects in 2D materials that has general implications for defect engineering in aqueous condition.
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| 37. |
- Zhang, Pan, et al.
(författare)
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Nanochannel-Based Transport in an Interfacial Memristor Can Emulate the Analog Weight Modulation of Synapses
- 2019
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Ingår i: Nano letters (Print). - : AMER CHEMICAL SOC. - 1530-6984 .- 1530-6992. ; 19:7, s. 4279-4286
-
Tidskriftsartikel (refereegranskat)abstract
- By exploiting novel transport phenomena such as ion selectivity at the nanoscale, it has been shown that nanochannel systems can exhibit electrically controllable conductance, suggesting their potential use in neuromorphic devices. However, several critical features of biological synapses, particularly their conductance modulation, which is both memorable and gradual, have rarely been reported in these types of systems due to the fast flow property of typical inorganic electrolytes. In this work, we demonstrate that electrically manipulating the nanochannel conductance can result in nonvolatile conductance tuning capable of mimicking the analog behavior of synapses by introducing a room-temperature ionic liquid (IL) and a KCl solution into the two ends of a nanochannel system. The gradual conductance-tuning mechanism is identified through fluorescence measurements as the voltage-induced movement of the interface between the immiscible IL and KCl solution, while the successful memorization of the conductance tuning is ascribed to the large viscosity of the IL. We applied a nanochannel-based synapse to a handwritten digit-recognition task, reaching an accuracy of 94%. These promising results provide important guidance for the future design of nanochannel-based neuromorphic devices and the manipulation of nanochannel transport for computing.
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| 38. |
- Zhang, Shunming, et al.
(författare)
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Adherence to a healthy lifestyle including sleep and sedentary behaviors and risk of metabolic dysfunction-associated steatotic liver disease in Chinese adults
- 2024
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Ingår i: Preventive Medicine. - 0091-7435. ; 184
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Tidskriftsartikel (refereegranskat)abstract
- Objective: Various lifestyle factors including smoking, alcohol, physical activity, sedentary behavior, diet quality, sleep behavior, and overweight have been related to metabolic dysfunction-associated steatotic liver disease (MASLD); however, their joint impact on risk of MASLD is not well known. We prospectively investigated the association between a combination of lifestyle factors and risk of MASLD. Methods: This prospective cohort study included 13,303 participants (mean age: 39.1 ± 11.3 years, female: 60.1%) in China. A novel healthy lifestyle score was created combining seven healthy factors: not smoking, no alcohol intake, regular physical activity, short sedentary time, healthy diet, healthy sleep, and healthy weight. Incident MASLD cases were ascertained annually by liver ultrasound and cardiometabolic risk factors. Multivariable Cox proportional hazards regression models were used to estimate the association of healthy lifestyle score with risk of MASLD. Results: Within 48,036 person-years of follow-up, 2823 participants developed MASLD. After adjusting for age, sex, education, occupation, household income, personal and family history of disease, and total energy intake, compared with participants with 0–2 healthy lifestyle factors, the multivariable hazard ratios (95% confidence interval) of MASLD were 0.81 (0.73, 0.89), 0.67 (0.61, 0.75), and 0.55 (0.49, 0.62) for healthy lifestyle score of 3, 4, and 5–7, respectively (P for trend <0.0001). Such associations were consistent across subgroup and sensitivity analyses. Conclusion: Our results indicate that a higher healthy lifestyle score is associated with a lower risk of MASLD.
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| 39. |
- Zhang, Zhicheng, et al.
(författare)
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The Editorial
- 2023
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Ingår i: Chemistry - An Asian Journal. - : John Wiley & Sons. - 1861-4728 .- 1861-471X. ; 18:6
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Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)abstract
- Surface and interface engineering has been considered as a promising strategy to enhance the performance of catalysts towards CO2 reduction. In their editorial to this special collection, guest editors Zhicheng Zhang, Zhen Zhang, Zhenyu Sun, Shaoqi Zhan and Guoxiong Wang provide a brief overview of this field and highlight the state-of-the-art contributions featured in this special collection.
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| 40. |
- Zhu, Zhenyu, et al.
(författare)
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Coupling life prediction of bending very high cycle fatigue of completion strings made of different materials using deep wise separable convolution
- 2024
-
Ingår i: Fatigue & Fracture of Engineering Materials & Structures. - : WILEY. - 8756-758X .- 1460-2695.
-
Tidskriftsartikel (refereegranskat)abstract
- This article predicts bending very high cycle fatigue (VHCF) life of three typical nickel-based alloys SM2550, BG2532, and G3 used for completion strings. Fatigue tests were conducted on the three alloys using an ultrasonic fatigue system at a frequency of 20 kHz. The results showed that the fatigue strength ranges of the three alloys were markedly different, reflecting their different sensitivities to fatigue loading. Scanning electron microscope observations revealed numerous fatigue crack origins with internal decohesion in the fatigue source region. To achieve unified prediction of the fatigue life for the three alloys, a prediction model based on deep learning was built with inputs including fatigue initiation quantity, cleavage facet size, and other fatigue fracture characteristics. It was found that single source feature was insufficient to obtain satisfactory prediction accuracy for all alloys, while multifeature coupling integration could significantly improve the prediction precision, enabling reliable prediction of alloy fatigue life. This study provides new insights into bending VHCF life prediction. This article predicts bending VHCF life for three completion strings. Bending VHCF life model utilizing deep wise separable convolution was established. Deep learning can effectively integrate with bending VHCF analyses.
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| 41. |
- Zhu, Zhenyu, et al.
(författare)
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Origin of prestrain-induced cyclic-strain hardening: Multi-scale experimental characterizations and simulations of 7075 aluminum alloy
- 2024
-
Ingår i: Materials & design. - : ELSEVIER SCI LTD. - 0264-1275 .- 1873-4197. ; 238
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Tidskriftsartikel (refereegranskat)abstract
- The influence of prefabricated dislocation features induced by rate dependent prestrain on the post-cyclic process in 7075 aluminum alloy exhibits significant variations, which are of great importance in terms of concerns, designs, and discoveries. Considering strain rate dependent prestrain provides diversified hardening stimuli for the subsequent cyclic process. The maximum cyclic stress in the post-cyclic stage was maintained at the same level as the prestress with strain rates ranging from 10-4s-1 to 10-1s-1. Subsequently, by adjusting post-cycling stress amplitude, research was conducted on quasi-plastic amplitude cycle (QPC) and low plasticity amplitude cycle (LPC) loading conditions. Through experimental mechanism analysis, as well as verification through molecular dynamics and crystal plasticity simulations, prestrain induced by rapid strain rates enhanced the hardening during QPC, stemming from the effects of matrix reconstruction strengthening and wavy structured grain boundaries. However, prestrain induced by slow strain rates promoted the hardening during LPC, primarily arising from the non-uniform crystal structures within individual grains, which was achieved through the complex sub-crystal clusters at grain boundaries, along with intracrystal orderly slipping lattice. These findings offer new insights for the optimization of microstructural design through dislocation engineering.
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| 42. |
- Özcan, Orcun Orkan, et al.
(författare)
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Differential Coding Strategies in Glutamatergic and GABAergic Neurons in the Medial Cerebellar Nucleus
- 2020
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Ingår i: Journal of Neuroscience. - : NLM (Medline). - 0270-6474 .- 1529-2401. ; 40:1, s. 159-170
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Tidskriftsartikel (refereegranskat)abstract
- The cerebellum drives motor coordination and sequencing of actions at the millisecond timescale through adaptive control of cerebellar nuclear output. Cerebellar nuclei integrate high-frequency information from both the cerebellar cortex and the two main excitatory inputs of the cerebellum: the mossy fibers and the climbing fiber collaterals. However, how nuclear cells process rate and timing of inputs carried by these inputs is still debated. Here, we investigate the influence of the cerebellar cortical output, the Purkinje cells, on identified cerebellar nuclei neurons in vivo in male mice. Using transgenic mice expressing Channelrhodopsin2 specifically in Purkinje cells and tetrode recordings in the medial nucleus, we identified two main groups of neurons based on the waveform of their action potentials. These two groups of neurons coincide with glutamatergic and GABAergic neurons identified by optotagging after Chrimson expression in VGLUT2-cre and GAD-cre mice, respectively. The glutamatergic-like neurons fire at high rate and respond to both rate and timing of Purkinje cell population inputs, whereas GABAergic-like neurons only respond to the mean population firing rate of Purkinje cells at high frequencies. Moreover, synchronous activation of Purkinje cells can entrain the glutamatergic-like, but not the GABAergic-like, cells over a wide range of frequencies. Our results suggest that the downstream effect of synchronous and rhythmic Purkinje cell discharges depends on the type of cerebellar nuclei neurons targeted.
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