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- Beal, Jacob, et al.
(författare)
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Robust estimation of bacterial cell count from optical density
- 2020
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Ingår i: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
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Tidskriftsartikel (refereegranskat)abstract
- Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
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| 5. |
- Jung, Christian, et al.
(författare)
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A comparison of very old patients admitted to intensive care unit after acute versus elective surgery or intervention
- 2019
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Ingår i: Journal of critical care. - : W B SAUNDERS CO-ELSEVIER INC. - 0883-9441 .- 1557-8615. ; 52, s. 141-148
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Tidskriftsartikel (refereegranskat)abstract
- Background: We aimed to evaluate differences in outcome between patients admitted to intensive care unit (ICU) after elective versus acute surgery in a multinational cohort of very old patients (80 years; VIP). Predictors of mortality, with special emphasis on frailty, were assessed.Methods: In total, 5063 VIPs were induded in this analysis, 922 were admitted after elective surgery or intervention, 4141 acutely, with 402 after acute surgery. Differences were calculated using Mann-Whitney-U test and Wilcoxon test. Univariate and multivariable logistic regression were used to assess associations with mortality.Results: Compared patients admitted after acute surgery, patients admitted after elective surgery suffered less often from frailty as defined as CFS (28% vs 46%; p < 0.001), evidenced lower SOFA scores (4 +/- 5 vs 7 +/- 7; p < 0.001). Presence of frailty (CFS >4) was associated with significantly increased mortality both in elective surgery patients (7% vs 12%; p = 0.01), in acute surgery (7% vs 12%; p = 0.02).Conclusions: VIPs admitted to ICU after elective surgery evidenced favorable outcome over patients after acute surgery even after correction for relevant confounders. Frailty might be used to guide clinicians in risk stratification in both patients admitted after elective and acute surgery.
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| 6. |
- Marendziak, A., et al.
(författare)
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Residual gas in the vacuum system of the solaris 1.5 GeV electron storage ring
- 2017
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Ingår i: IPAC 2017 - Proceedings of the 8th International Particle Accelerator Conference. - 9783954501823 ; , s. 2734-2737
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Konferensbidrag (refereegranskat)abstract
- Solaris is a third generation light source constructed at the Jagiellonian University in Krakow, Poland. The machine was designed by the MAX IV Laboratory team. The replica of the 1.5 GeV storage ring with 96 m circumference of a vacuum system was successfully built and now the synchrotron facility is after the 3rd phase of commissioning. Recent installation of the Residual Gas Analyzer (RGA) in the storage ring allows now for evaluation of the residual gas composition. Within this paper the result of residual gas analysis in the vacuum system of storage ring during different states of the machine will be presented. Result of vacuum performance regarding beam cleaning and beam lifetime will be presented. Moreover, the NEG strips performance will be evaluated and reported.
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- Phillips, S. D., et al.
(författare)
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Exploring the role of phosphorus substituents on the enantioselectivity of Ru-catalysed ketone hydrogenation using tridentate phosphine-diamine ligands
- 2011
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Ingår i: Catalysis Science and Technology. - : Royal Society of Chemistry (RSC). - 2044-4753 .- 2044-4761. ; 1:8, s. 1336-1339
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Tidskriftsartikel (refereegranskat)abstract
- Methods to produce a range of new phosphine-diamine ligands from phosphino-aldehydes have been developed and a hypothesis that larger P-substituents would increase the enantioselectivity towards the (S) isomer in Ru-catalysed ketone hydrogenation of acetophenone has been examined; the successful validation of this hypothesis is further evidence that the mechanism of these catalysts involves a secondary amine assisted reduction.
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| 8. |
- Sliwa, E, et al.
(författare)
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Influence of dexamethasone and alpha-ketoglutarate on growth and development of the skeletal system of pigs
- 2005
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Ingår i: Medycyna Weterynaryjna. - 0025-8628. ; 61:5, s. 526-531
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Tidskriftsartikel (refereegranskat)abstract
- The aim of the study was to establish the influence of dexamethasone and/or alpha-ketoglutarate (AKG) on the growth, mineralization, geometric and mechanical properties of ribs as a methodological model. The experiment was conducted during the final 24 days of prenatal life and first 14 days of neonatal life. The volumetric bone density of the trabecular and cortical bone was determined separately using the quantitative computed tomography (QCT) method and Somatom AR. T - Siemens apparatus. The mechanical properties were determined using the three-point bending test according to the method by Ferretti et al. The obtained results indicate that maternal administration of dexamethasone increased volumetric mineral density, although the mechanical parameters had lower values. Prenatal administration of AKG decreased all the geometric and mechanical parameters of the investigated ribs. The results obtained during the administration of the compound in neonatal life were the opposite of the prenatal results. Dexamethasone and AKG given together had the same influence on mineralization and other properties AND increasing their values during prenatal and neonatal life. This model of administrating dexamethasone and AKG maternally during prenatal life with the intention of provoking structural growth of the skeleton may be useful in further elucidating some biological and pharmacological factors on bone formation in prenatal life.
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