| 1. |
- Ananthaseshan, S., et al.
(författare)
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Red blood cell distribution width is associated with increased interactions of blood cells with vascular wall
- 2022
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Ingår i: Scientific Reports. - : Springer Nature. - 2045-2322. ; 12:1
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Tidskriftsartikel (refereegranskat)abstract
- The mechanism underlying the association between elevated red cell distribution width (RDW) and poor prognosis in variety of diseases is unknown although many researchers consider RDW a marker of inflammation. We hypothesized that RDW directly affects intravascular hemodynamics, interactions between circulating cells and vessel wall, inducing local changes predisposing to atherothrombosis. We applied different human and animal models to verify our hypothesis. Carotid plaques harvested from patients with high RDW had increased expression of genes and proteins associated with accelerated atherosclerosis as compared to subjects with low RDW. In microfluidic channels samples of blood from high RDW subjects showed flow pattern facilitating direct interaction with vessel wall. Flow pattern was also dependent on RDW value in mouse carotid arteries analyzed with Magnetic Resonance Imaging. In different mouse models of elevated RDW accelerated development of atherosclerotic lesions in aortas was observed. Therefore, comprehensive biological, fluid physics and optics studies showed that variation of red blood cells size measured by RDW results in increased interactions between vascular wall and circulating morphotic elements which contribute to vascular pathology.
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| 2. |
- Bednar, Peter, et al.
(författare)
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Contextual Inquiry and Requirements Shaping
- 2009
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Ingår i: The Inter-Networked World: ISD Theory, Practice, and Education. - 9780387304038 ; 1, s. 225-236
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Bokkapitel (refereegranskat)abstract
- A primary purpose of traditional systems analysis is seen as ‘capture’ or ‘elicitation’ of user requirements, in order to produce specifications as a basis for information systems design. Such a view presupposes that user requirements are pre-existing and clear, and that the particular ‘users’ concerned know what they are, and can therefore articulate them. We would argue that none of these assumptions can be taken for granted. If a system is to be created which is useful to particular individuals, we suggest that they need to take ownership and control of the analysis themselves. By exploring their own experiences, aspirations and sense-making processes in the context of their problem space, they may enable richer and more comprehensive understandings to emerge. A creative process of requirements shaping may then be promoted. Our focus, therefore, moves away from problem description by an external analyst, towards contextual inquiry, which supports creative thinking and problem re-definition by those individuals most affected. We will discuss methods which may facilitate exploration of multiple, simultaneous and dynamic roles of the same autonomous individuals, separately and collectively. Such methods may enable emergence of reflective, shifting perspectives, leading to deepened understandings of problem experiences. This approach makes it possible for resolutions to be created that address experiences, rather than descriptions, of problems.
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