| 1. |
- Beal, Jacob, et al.
(författare)
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Robust estimation of bacterial cell count from optical density
- 2020
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Ingår i: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
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Tidskriftsartikel (refereegranskat)abstract
- Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
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| 2. |
- Klionsky, Daniel J., et al.
(författare)
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Guidelines for the use and interpretation of assays for monitoring autophagy
- 2012
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Ingår i: Autophagy. - : Informa UK Limited. - 1554-8635 .- 1554-8627. ; 8:4, s. 445-544
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Forskningsöversikt (refereegranskat)abstract
- In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process vs. those that measure flux through the autophagy pathway (i.e., the complete process); thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from stimuli that result in increased autophagic activity, defined as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (in most higher eukaryotes and some protists such as Dictyostelium) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the field understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field.
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| 3. |
- Chen, Ling, et al.
(författare)
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Assessment on abrasiveness of high chromium cast iron material on the wear performance of PCBN cutting tools in dry machining
- 2018
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Ingår i: Journal of Materials Processing Technology. - : Elsevier BV. - 0924-0136. ; 255, s. 110-120
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Tidskriftsartikel (refereegranskat)abstract
- Polycrystalline Cubic Boron Nitride (PCBN) has been considered as the main cutting tool material for hard turning, which has the comparable surface finish quality as grinding, and high efficiency, economical technology in production. It is well known that the abrasiveness of the workpiece have the very important role of the machinability of the materials. Therefore, the tool wear of the PCBN insert correlates with abrasiveness of the materials very much. In the presented paper, the influence of the micro hardness distribution is used to explain the wear mechanism in machining of the high abrasive materials and proposed a model to predict the abrasiveness. The work material is high chromium cast iron and cutting tool material is PCBN. Abrasiveness are evaluated from the micro hardness distribution by nanoindentation for the distinguish microstructure with hardness. The mapping image of the micro hardness showed the matched features with microstructure images from the scanning electron microscope (SEM). The different chemical composition groups are presented as three heat treatment conditions in experiments, which are as-cast, annealed and hardened. Based on the hardness distribution, this paper presented a model to evaluate the abrasiveness index of the material. The high performance machining is done on six material. The wear mechanism is abrasion in tool wear dominantly and flank wear showed the linear relationship with abrasiveness index. The abrasive wear on the cutting edge suggests that sliding and chipping are the main wear modes which is caused by the combination effect of carbides and matrix. The four mechanisms of diffusion, chemical, abrasive and micro chipping are happening in the tool wear of hard turning. In the dry machining by PCBN insert, abrasiveness is correlated with abrasive and chipping very much and accelerated the crater wear in machining. Abrasiveness index of the material has ability to predict wear performance in dry machining of HCWCI materials by PCBN inserts. The application of the abrasiveness is a potential parameter of machinability to improve the machining efficiency and reduce the cost of machining.
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| 4. |
- Guo, Xin, et al.
(författare)
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Machinability study on dry machining of white cast iron by polycrystalline cubic boron nitride inserts
- 2022
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Ingår i: Machining Science and Technology. - : Informa UK Limited. - 1091-0344 .- 1532-2483. ; 26:2, s. 137-159
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Tidskriftsartikel (refereegranskat)abstract
- The polycrystalline cubic boron nitride (PCBN) insert has been widely used in machining high chromium white cast iron (HCCI), which has the high hardness and abrasiveness. The aim of this article is to reveal that micro-mechanical properties play an essential role in assessing the machinability of high chromium cast iron. Grid nanoindentation is applied to measure the micro-hardness distribution. Based on grid nanoindentation results, the micro-hardness distribution is proposed to study the machinability. After the machining test of HCCI by the PCBN insert, the cutting force, tool wear, surface roughness, and chip formation showed that the micro-hardness distribution of materials has the better results than macro-hardness in machinability evaluation, and abrasive wear occurred on the rake and flank face of the cutting tool. The abrasiveness index that is related with the micro-hardness distribution and the abrasive wear effect in machining, for the most of high abrasion materials, has the possible to evaluate the dynamic cutting process and tool life in dry machining by using the PCBN insert. The micro-hardness distribution imported into the machinability model may propose a new way to improve the data exchange capability in the modern manufacturing process.
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| 5. |
- Liu, Chang, et al.
(författare)
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Oligomer Dynamics of LL-37 Truncated Fragments Probed by α-Hemolysin Pore and Molecular Simulations
- 2023
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Ingår i: Small. - 1613-6810 .- 1613-6829. ; 19:37
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Tidskriftsartikel (refereegranskat)abstract
- Oligomerization of antimicrobial peptides (AMPs) is critical in their effects on pathogens. LL-37 and its truncated fragments are widely investigated regarding their structures, antimicrobial activities, and application, such as developing new antibiotics. Due to the small size and weak intermolecular interactions of LL-37 fragments, it is still elusive to establish the relationship between oligomeric states and antimicrobial activities. Here, an α-hemolysin nanopore, mass spectrometry (MS), and molecular dynamic (MD) simulations are used to characterize the oligomeric states of two LL-37 fragments. Nanopore studies provide evidence of trapping events related to the oligomer formation and provide further details on their stabilities, which are confirmed by MS and MD simulations. Furthermore, simulation results reveal the molecular basis of oligomer dynamics and states of LL-37 fragments. This work provides unique insights into the relationship between the oligomer dynamics of AMPs and their antimicrobial activities at the single-molecule level. The study demonstrates how integrating methods allows deciphering single molecule level understanding from nanopore sensing approaches.
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| 6. |
- Melaibari, Ammar, et al.
(författare)
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Extreme Hardness Achievements in Binderless Cubic Boron Nitride Tools
- 2013
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Ingår i: Transactions of the North American Manufacturing Research Institution of SME Volume 41, 2013. - 9781627486972 ; , s. 449-457
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Konferensbidrag (refereegranskat)abstract
- Binderless cubic boron nitride tools are available in two forms: single phase cBN and dual phase wBN/cBN (w is wurtzite phase). In this work, a novel heat treatment process involving surface heating using a continuous wave CO2 laser followed by tandem waterjet quenching of the laser beam path was applied to increase the hardness of both forms of boron nitride. Stress-induced phase transitions and nanometric grain sizes accompanying the rapid quench heat treatment enabled a hardness increase of 20% in single phase cBN (nominal 60 GPa) and 100% in dual phase wBN/cBN (nominal 75 GPa) that attest the ability of cBN to reach the hardness of polycrystalline diamond (65-80 GPa). The effects of laser heat treatment are identified by an examination of the changes in phase and microstructure by Raman spectroscopy, high resolution scanning electron microscopy and X-ray diffraction.
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| 7. |
- Sjökvist, Linnea, et al.
(författare)
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Numerical models for the motion and forces of point-absorbing wave energy converters in extreme waves
- 2017
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Ingår i: Ocean Engineering. - : Elsevier BV. - 0029-8018 .- 1873-5258. ; 145, s. 1-14
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Tidskriftsartikel (refereegranskat)abstract
- Reliable simulation tools are necessary to study the performance and survivability of wave energy devices, since experiments are both expensive and difficult to implement. In particular, survivability in nonlinear, high waves is one of the largest challenges for wave energy, and since the wave loads and dynamics are largely model dependent, each device must be studied separately with validated tools. In this paper, two numerical methods based on fully nonlinear computational fluid dynamics (CFD) are presented and compared with a simpler linear method. All three methods are compared and validated against experimental data for a point-absorbing wave energy converter in nonlinear, high waves. The wave energy converter consists of a floating buoy attached to a linear generator situated on the seabed. The line forces and motion of the buoy are studied, and computational cost and accuracy are compared and discussed. Whereas the simpler linear method is very fast, its accuracy is not sufficient in high and extreme waves, where instead the computationally costly CFD methods are required. The OpenFOAM model showed the highest accuracy, but also a higher computational cost than the ANSYS Fluent model.
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| 8. |
- Wei, Ting, et al.
(författare)
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Developed and developing world responsibilities for historical climate change and CO2 mitigation
- 2012
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Ingår i: Proceedings of the National Academy of Sciences of the United States of America. - : Proceedings of the National Academy of Sciences. - 0027-8424 .- 1091-6490. ; 109:32, s. 12911-12915
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Tidskriftsartikel (refereegranskat)abstract
- At the United Nations Framework Convention on Climate Change Conference in Cancun, in November 2010, the Heads of State reached an agreement on the aim of limiting the global temperature rise to 2 degrees C relative to preindustrial levels. They recognized that long-term future warming is primarily constrained by cumulative anthropogenic greenhouse gas emissions, that deep cuts in global emissions are required, and that action based on equity must be taken to meet this objective. However, negotiations on emission reduction among countries are increasingly fraught with difficulty, partly because of arguments about the responsibility for the ongoing temperature rise. Simulations with two earth-system models (NCAR/CESM and BNU-ESM) demonstrate that developed countries had contributed about 60-80%, developing countries about 20-40%, to the global temperature rise, upper ocean warming, and sea-ice reduction by 2005. Enacting pledges made at Cancun with continuation to 2100 leads to a reduction in global temperature rise relative to business as usual with a 1/3-2/3 (CESM 33-67%, BNU-ESM 35-65%) contribution from developed and developing countries, respectively. To prevent a temperature rise by 2 degrees C or more in 2100, it is necessary to fill the gap with more ambitious mitigation efforts.
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| 9. |
- Wu, Jinming, et al.
(författare)
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Identifying the role of co-aggregation of Alzheimer's amyloid-beta with amorphous protein aggregates of non-amyloid proteins
- 2022
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Ingår i: Cell Reports Physical Science. - : Elsevier BV. - 2666-3864. ; 3:9
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Tidskriftsartikel (refereegranskat)abstract
- Protein homeostasis collapse typically leads to protein aggregation into amyloid fibrils and diffuse amorphous aggregates, which both occur in Alzheimer’s and other neurodegenerative diseases, but their relationship remains to be clarified. Here we examine the interactions between the amorphously aggregated non-chaperone proteins (albumin, β-lactoglobulin, and superoxide dismutase 1) and Alzheimer’s amyloid-β (Aβ) peptides. Amorphous aggregates suppress the primary nucleation and elongation of Aβ fibrillation and modulate Aβ toxicity. The higher inhibitory efficiency of intermediately sized molten globular aggregates (20–300 nm) on Aβ fibrillation is hypothesized to be due to the higher amount of exposed hydrophobic residues and higher free energy. The formed co-aggregates are off-pathway species that favor formation of the amorphous end state instead of fibrillar amyloid structures normally formed by Aβ. Our findings expand our knowledge of how the native and aggregated cellular proteins modulate Aβ aggregation at the molecular and mesoscopic level and point out the major conclusions.
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| 10. |
- Wu, Jinming, et al.
(författare)
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Investigation on the wave energy converter that reacts against an internal inverted pendulum
- 2022
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Ingår i: Energy. - : Elsevier. - 0360-5442 .- 1873-6785. ; 247
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Tidskriftsartikel (refereegranskat)abstract
- In this work, a wave energy converter (WEC) that reacts against an internal inverted pendulum, which works as an inertial device to provide reaction for power absorption and is potentially superior due to its natural high elevation of the internal mass compared to a normal pendulum, named IPWEC has been studied. Optimal structural configurations of the WEC have been identified by a genetic algorithm. The equations of motion have been defined and solved explicitly using a linearized model, which has been validated by experiments and a non-linearized model. When comparing IPWEC with the WEC that reacts against a normal pendulum (NPWEC), it is found that, although both WECs present almost the same wave power capture ability, IPWEC possesses several advantages in most sea states due to the naturally high elevation of the pendulum's center of gravity: (1) the pendulum mass and the angular motion amplitude of the pendulum are 35% and 50%, respectively, smaller than those of NPWEC; (2) the averaged reactive power required under complex conjugate control is 75% smaller than NPWEC; (3) the moment which holds the pendulum fixed relative to the hull in the survival mode is merely a half as large as that of NPWEC.
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| 11. |
- Wu, Jinming, et al.
(författare)
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Latching and Declutching Control of the Solo Duck Wave-Energy Converter with Different Load Types
- 2017
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Ingår i: Energies. - : MDPI AG. - 1996-1073. ; 10:12
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Tidskriftsartikel (refereegranskat)abstract
- The solo duck wave-energy converter (WEC) captures power in a point absorber manner, hence it exhibits high power-capture efficiency within only a narrow bandwidth. Passive control is characterized by a unidirectional power flow, and thus its engineering implementation can be simplified. In this paper, two typical passive control strategies, latching and declutching control, are applied to the solo duck WEC to improve its power-capture performance at wave periods larger and smaller than the natural period of the WEC, respectively. Special attention is paid to the peak value of instantaneous WEC performance parameters, including the peak motion excursion, the peak power take-off (PTO) moment, and the peak-to-average power ratio, when the captured power is maximized. Performance differences between the linear and coulomb loads are also investigated. Results show that both latching and declutching control can effectively improve captured power, but also incidentally increase the peak motion excursion and peak-to-average power ratio. When under latching and declutching control, the coulomb load leads to the same maximum relative capture width and peak motion excursion as the linear load, but presents smaller peak PTO moment and peak-to-average power ratio than the linear load, hence making the coulomb load the better choice for the solo duck WEC.
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| 12. |
- Wu, Jinming, et al.
(författare)
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Numerical and Experimental Study of the Solo Duck Wave Energy Converter
- 2019
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Ingår i: Energies. - : MDPI. - 1996-1073. ; 12:10
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Tidskriftsartikel (refereegranskat)abstract
- The Edinburgh Duck is one of the highly-efficient wave energy converters (WECs). Compared to the spine-connected Duck configuration, the solo Duck will be able to use the point absorber effect to enhance its power capture performance. In this paper, a 3D computational fluid dynamic (CFD) model is developed to predict the hydrodynamic performance of the solo Duck WEC in regular waveswithin a wide range ofwave steepness until the Duck capsizes. A set of experiments was designed to validate the accuracy of the CFD model. Boundary element method (BEM) simulations are also performed for comparison. CFD results agree well with experimental results and the main difference comes from the friction in the mechanical transmission system. CFD results also agree well with BEM results and differences appear at large wave steepness as a result of two hydrodynamic nonlinear factors: the nonlinear waveform and the vortex generation process. The influence of both two nonlinear factors iscombined to be quantitatively represented by the drag torque coefficient.The vortex generation process is found to cause a rapid drop of the pressure force due to the vortexes taking away the kinetic energy from the fluid.
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| 13. |
- Wu, Jinming, et al.
(författare)
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Optimizing the Performance of Solo Duck Wave Energy Converter in Tide
- 2017
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Ingår i: Energies. - : MDPI AG. - 1996-1073. ; 10:3
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Tidskriftsartikel (refereegranskat)abstract
- The high efficiency performance of the Edinburgh Duck wave energy converter (WEC) in 2D regular wave tests makes it a promising wave energy conversion scheme. A solo Duck WEC will be able to apply the point absorber effect to further enhance its performance. Since released degree of freedom will decrease the efficiency, a Duck WEC with fixed pitching axis will be a better option. However, for fixed supported WECs, tide is a non-ignorable consideration. In this paper, a movable mass method is utilized in the whole tidal range to not only balance the Duck to appropriate beak angles, but also follow the variation of hydrodynamic coefficients to keep cancelling the reactance of the system impedance so that complex conjugate control can be realized to optimize the power capture performance of the Duck WEC in tide. Results show that the beak angle should be adjusted to as large a value as possible so that the response amplitude of the Duck at maximum relative capture width will be reasonable small, and the lowest weight of the movable mass is found when its designed position locates at the center of the Duck profile.
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| 14. |
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| 15. |
- Wu, Jinming, et al.
(författare)
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Performance analysis of solo Duck wave energy converter arrays under motion constraints
- 2017
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Ingår i: Energy. - : Elsevier BV. - 0360-5442 .- 1873-6785. ; 139, s. 155-169
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Tidskriftsartikel (refereegranskat)abstract
- This paper studies the power capture performance of solo Duck wave energy converter (WEC) arrays. The barrier function method combined with a quasi-Newton BFGS optimization algorithm is applied to find the maximum captured power of the array when the Ducks are under motion constraints. Based on this optimized maximum captured power, the effects of separation distance, wave period, incident wave direction and Duck width on the array performance are investigated. For the two Ducks array, results show that the alternative constructive and destructive interaction stripes in the contour plot of the q-factor variation with non-dimensional separation distance are resulted from the diffracted wave pattern from each Duck, and the hydrodynamic interaction strength is reduced when constraints affect the performance. For the three Ducks array, the middle Duck shows larger variability of captured power than the side Ducks due to experiencing double in phase diffracted wave from the side ones. The captured power of the solo Duck WEC array is sensitive to incident wave direction, and arrays with Ducks of smaller width are found to have better performance in power capture efficiency.
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| 16. |
- Wu, Jinming, et al.
(författare)
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Real-time latching control strategies for the solo Duck wave energy converter in irregular waves
- 2018
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Ingår i: Applied Energy. - : Elsevier. - 0306-2619 .- 1872-9118. ; 222, s. 717-728
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Tidskriftsartikel (refereegranskat)abstract
- As a point absorber, the solo Duck wave energy converter (WEC) shows high power capture efficiency within a narrow bandwidth around the natural period. In this paper, real-time latching control is applied to the solo Duck WEC in irregular waves to improve its performance in sea states away from the natural period. Two predictive latching control strategies, in which one is close-to-optimal and the other is sub-optimal, and one non-predictive strategy are considered. The improvement of the WEC performance due to latching control is studied. Compared to the performance under simple resistive control, the three latching control strategies show almost equivalent control effect, leading to an average increase of the maximum relative capture width by around 70% and an average decrease of the optimal power take-off (PTO) damping coefficient by around 60%. Since the non-predictive strategy requires no prediction of future excitation force and WEC motion, it can be identified as the best choice for the solo Duck WEC under latching control. Although latching control leads to significant increase of fatigue load on the WEC hull like other advanced controls, it does not cause additional fatigue damage to the PTO.
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| 17. |
- Wärmländer, Sebastian K. T. S., et al.
(författare)
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Metal binding to the amyloid-beta peptides in the presence of biomembranes : potential mechanisms of cell toxicity
- 2019
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Ingår i: Journal of Biological Inorganic Chemistry. - : Springer Science and Business Media LLC. - 0949-8257 .- 1432-1327. ; 24:8, s. 1189-1196
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Forskningsöversikt (refereegranskat)abstract
- The amyloid-beta (A beta) peptides are key molecules in Alzheimer's disease (AD) pathology. They interact with cellular membranes, and can bind metal ions outside the membrane. Certain oligomeric A beta aggregates are known to induce membrane perturbations and the structure of these oligomers-and their membrane-perturbing effects-can be modulated by metal ion binding. If the bound metal ions are redox active, as e.g., Cu and Fe ions are, they will generate harmful reactive oxygen species (ROS) just outside the membrane surface. Thus, the membrane damage incurred by toxic A beta oligomers is likely aggravated when redox-active metal ions are present. The combined interactions between A beta oligomers, metal ions, and biomembranes may be responsible for at least some of the neuronal death in AD patients.
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| 18. |
- Zha, Jinlin, et al.
(författare)
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Attribution of Terrestrial Near-Surface Wind Speed Changes Across China at a Centennial Scale
- 2024
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Ingår i: Geophysical Research Letters. - 0094-8276 .- 1944-8007. ; 51
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Tidskriftsartikel (refereegranskat)abstract
- Near-surface wind speed (NSWS) over China shows multiple time-scale changes at a centennial scale, but the contributions of internal variability (IV), anthropogenic forcing (ANT), and natural forcing (NAT) to those changes remain unknown. This study investigated the contributions of IV, ANT, and NAT to NSWS changes at a centennial scale. Results show that the NSWS changes were attributed mainly to IV. IV not only modulated the interannual changes in NSWS but also determined the interdecadal transition in NSWS. The relative contributions of IV to the interannual and decadal NSWS exceeded 75.0%. ANT contributed particularly to the long-term reduction in NSWS; especially, it has contributed 55.0% of the reduction in NSWS since 1957, serving as the major contributor to the reduction in NSWS. NAT had a small-to-negligible effect on China's NSWS throughout the study period. This study enhances our understanding of NSWS changes at different time scales.
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| 19. |
- Zhang, Kaicheng, et al.
(författare)
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SN 2014J in M82 : new insights on the spectral diversity of Type Ia supernovae
- 2018
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Ingår i: Monthly notices of the Royal Astronomical Society. - : Oxford University Press (OUP). - 0035-8711 .- 1365-2966. ; 481:1, s. 878-893
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Tidskriftsartikel (refereegranskat)abstract
- We present extensive spectroscopic observations for one of the closest Type Ia supernovae (SNe Ia), SN 2014J discovered in M82, ranging from 10.4 d before to 473.2 d after B-band maximum light. The diffuse interstellar band features detected in a high-resolution spectrum allow an estimate of line-of-sight extinction as A(v) similar to 1.9 +/- 0.6 mag. Spectroscopically, SN 2014J can be put into the high-velocity (HV) subgroup in Wang's classification with a velocity of Si II lambda 6355 at maximum light of upsilon(0) = 1.22 +/- 0.01 x 10(4) km s(-1) but has a low velocity gradient (LVG, following Benetti's classification) of (v) over bar = 41 +/- 2 km s(-1) d(-1), which is inconsistent with the trend that HV SNe Ia generally have larger velocity gradients. We find that the HV SNe Ia with LVGs tend to have relatively stronger Si III (at similar to 4400 angstrom) absorptions in early spectra, larger ratios of S II lambda 5468 to S II lambda 5640, and weaker Si II 5972 absorptions compared to their counterparts with similar velocities but high velocity gradients. This shows that the HV+ LVG subgroup of SNe Ia may have intrinsically higher photospheric temperature, which indicates that their progenitors may experience more complete burning in the explosions relative to the typical HV SNe Ia.
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