| 1. |
- Haraguchi, Hideyuki, et al.
(författare)
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One-year outcomes of low-intensity behavioral interventions among Japanese preschoolers with autism spectrum disorders : Community-based study
- 2020
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Ingår i: Research in Autism Spectrum Disorders. - : Elsevier. - 1750-9467 .- 1878-0237. ; 76
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Tidskriftsartikel (refereegranskat)abstract
- Despite the need for evidence-based practice for children with autism spectrum disorders in the community, especially in areas with a scarcity of qualified experts and high financial costs, there is a lack of evidence concerning the potential benefits of early interventions which may be affordable in the real world (e.g. low-intensity behavioral or eclectic interventions). This study examined behavioral changes in preschool-aged children with autism spectrum disorders who received low-intensity behavioral interventions (a mean of 5.5 h per week) or non-behavioral eclectic interventions delivered in community settings in Japan across a 1-year period and compared outcomes between the groups. We assessed children’s developmental quotient, adaptive behavior, autism symptom/severity, and maternal stress and depression at baseline and after 1 year. Our results revealed that children receiving interventions in their communities showed improvement in language and social development. Further, the degree of improvement in children receiving low-intensity behavioral interventions was significantly greater than in children receiving non-behavioral eclectic interventions. The improvement was associated with the intensity of total, especially one-to-one interventions. On the other hand, there were no group differences in the degree of improvement in other outcome measures. Our results suggest that some preschool-aged children with autism spectrum disorder may benefit from low-intensity behavioral interventions for their language and social development. A greater intensity of one-to-one interventions was found to be associated with greater progress in language development and communication skills. The current study highlights the importance of implementing early interventions in the community, even at a low intensity.
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| 2. |
- Horie, Masafumi, et al.
(författare)
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Integrative CAGE and DNA Methylation Profiling Identify Epigenetically Regulated Genes in NSCLC
- 2017
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Ingår i: Molecular Cancer Research. - : AMER ASSOC CANCER RESEARCH. - 1541-7786 .- 1557-3125. ; 15:10, s. 1354-1365
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Tidskriftsartikel (refereegranskat)abstract
- Lung cancer is the leading cause of cancer-related deaths worldwide. The majority of cancer driver mutations have been identified; however, relevant epigenetic regulation involved in tumorigenesis has only been fragmentarily analyzed. Epigenetically regulated genes have a great theranostic potential, especially in tumors with no apparent driver mutations. Here, epigenetically regulated genes were identified in lung cancer by an integrative analysis of promoter-level expression profiles from Cap Analysis of Gene Expression (CAGE) of 16 nonsmall cell lung cancer (NSCLC) cell lines and 16 normal lung primary cell specimens with DNA methylation data of 69 NSCLC cell lines and 6 normal lung epithelial cells. A core set of 49 coding genes and 10 long noncoding RNAs (lncRNA), which are upregulated in NSCLC cell lines due to promoter hypomethylation, was uncovered. Twenty-two epigenetically regulated genes were validated (upregulated genes with hypomethylated promoters) in the adenocarcinoma and squamous cell cancer subtypes of lung cancer using The Cancer Genome Atlas data. Furthermore, it was demonstrated that multiple copies of the REP522 DNA repeat family are prominently upregulated due to hypomethylation in NSCLC cell lines, which leads to cancer-specific expression of lncRNAs, such as RP1-90G24.10, AL022344.4, and PCAT7. Finally, Myeloma Overexpressed (MYEOV) was identified as the most promising candidate. Functional studies demonstrated that MYEOV promotes cell proliferation, survival, and invasion. Moreover, high MYEOV expression levels were associated with poor prognosis. (C) 2017 AACR.
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| 3. |
- Horie, Masafumi, et al.
(författare)
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Transcriptome analysis of periodontitis-associated fibroblasts by CAGE sequencing identified DLX5 and RUNX2 long variant as novel regulators involved in periodontitis
- 2016
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Ingår i: Scientific Reports. - : Springer Science and Business Media LLC. - 2045-2322. ; 6
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Tidskriftsartikel (refereegranskat)abstract
- Periodontitis is affecting over half of the adult population, and represents a major public health problem. Previously, we isolated a subset of gingival fibroblasts (GFs) from periodontitis patients, designated as periodontitis-associated fibroblasts (PAFs), which were highly capable of collagen degradation. To elucidate their molecular profiles, GFs isolated form healthy and periodontitis-affected gingival tissues were analyzed by CAGE-seq and integrated with the FANTOM5 atlas. GFs from healthy gingival tissues displayed distinctive patterns of CAGE profiles as compared to fibroblasts from other organ sites and characterized by specific expression of developmentally important transcription factors such as BARX1, PAX9, LHX8, and DLX5. In addition, a novel long non-coding RNA associated with LHX8 was described. Furthermore, we identified DLX5 regulating expression of the long variant of RUNX2 transcript, which was specifically active in GFs but not in their periodontitis-affected counterparts. Knockdown of these factors in GFs resulted in altered expression of extracellular matrix (ECM) components. These results indicate activation of DLX5 and RUNX2 via its distal promoter represents a unique feature of GFs, and is important for ECM regulation. Down-regulation of these transcription factors in PAFs could be associated with their property to degrade collagen, which may impact on the process of periodontitis.
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| 4. |
- Kolstad, Markus, et al.
(författare)
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Integrating Socially Assistive Robots into Japanese Nursing Care
- 2020
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Ingår i: Digital Personalized Health and Medicine. - : IOS Press. - 9781643680828 - 9781643680835 ; , s. 1323-1324
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Konferensbidrag (refereegranskat)abstract
- This paper presents experiences of integrating assistive robots in Japanese nursing care through semi-structured interviews and site observations at three nursing homes in Japan during the spring of 2019. The study looked at experiences with the robots Paro, Pepper, and Qoobo. The goal was to investigate and evaluate the current state of using robots in the nursing care context, firsthand experiences with intended and real use, as well as response from the elderly and nursing staff. The qualitative analysis results pointed out user satisfaction, adjusted purpose, therapeutic and entertaining effects. Potentials of robots to assist in elderly care is advantageous. Limitations currently relate to the lack of ways to fully utilized and integrate robots.
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| 5. |
- Kolstad, Markus, et al.
(författare)
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Integrating Socially Assistive Robots into Japanese Nursing Care
- 2020
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Ingår i: The Importance of Health Informatics in Public Health during a Pandemic. - : IOS Press. - 9781643680927 - 9781643680934 ; , s. 183-186
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Konferensbidrag (refereegranskat)abstract
- This paper presents experiences of integrating assistive robots in Japanese nursing care through semi-structured interviews and site observations at three nursing homes in Japan during the year 2019. The study looked at experiences with the robots Paro, Pepper, and Qoobo. The goal was to investigate and evaluate the current state of using robots within the nursing care context, which involved: firsthand experiences with intended and real users; and response from the elderly, and nursing staff. The qualitative analysis results pointed out user satisfaction, adjusted purpose, therapeutic and entertaining effects. The potentials of using robots to assist in elderly care has been evident. Limitations currently relate to the lack of ways to fully utilize and integrate robots.
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| 6. |
- Ohshima, Mitsuhiro, et al.
(författare)
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bFGF rescues imatinib/STI571-induced apoptosis of sis-NIH3T3 fibroblasts.
- 2009
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Ingår i: Biochemical and Biophysical Research Communications - BBRC. - : Elsevier BV. - 0006-291X .- 1090-2104. ; 381:2, s. 165-170
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Tidskriftsartikel (refereegranskat)abstract
- PDGF-B-transfected, sis-NIH3T3 fibroblasts serve as a model system for examining the role of PDGF signaling in tumors. We have found that imatinib/STI571, a tyrosine kinase inhibitor targeting PDGF receptors, induces apoptosis of sis-NIH3T3 fibroblasts cultured under serum free conditions, which was rescued by the addition of 10% newborn calf serum (NCS). Therefore, growth factors included in serum were tested with regard to their ability to rescue imatinib-induced apoptosis. While PDGF-AB, EGF, and IGF-I failed to protect imatinib-induced sis-NIH3T3 cell apoptosis, bFGF rescued it. The effects of bFGF were confirmed by both cell viability assays and Bax/Bcl-2 gene expression ratio. An FGF receptor inhibitor, PD166866, invalidated the protective effect of bFGF. However, combination of imatinib and PD166866 failed to induce cell death of sis-NIH3T3 cells when cultured in 10% NCS. These results indicate that synergistic administration of some types of tyrosine kinase inhibitors need to be tested under in vivo-like conditions to establish novel strategies in anti-cancer therapy.
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| 7. |
- Ohshima, Mitsuhiro, et al.
(författare)
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Fibroblast VEGF-receptor 1 expression as molecular target in periodontitis
- 2016
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Ingår i: Journal of Clinical Periodontology. - : Wiley. - 0303-6979 .- 1600-051X. ; 43:2, s. 128-137
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Tidskriftsartikel (refereegranskat)abstract
- AimDegradation of extracellular matrices is an integral part in periodontitis. For antagonizing this pathophysiological mechanism, we aimed at identifying gene expression profiles in disease progression contributing periodontitis-associated fibroblasts (PAFs) versus normal gingival fibroblasts to determine their molecular repertoire, and exploit it for therapeutic intervention. Materials and MethodsApplying an exploratory analysis using a small number of microarrays in combination with a three dimensional (3D) invitro culture model that incorporates some aspects of periodontitis, PAFs were initially characterized by gene-expression analyses, followed by targeted gene down-regulation and pharmacological intervention in vitro. Further, immunohistochemistry was applied for phosphorylation analyses in tissue specimens. ResultsPAFs were characterized by 42 genes being commonly up-regulated >1.5-fold, and by five genes that were concordantly down-regulated (<0.7-fold). Expression of vascular endothelial growth factor (VEGF)-receptor 1 (Flt-1) was highly enhanced, and was thus further explored in invitro culture models of periodontal fibroblasts without accounting for the microbiome. Phosphorylation of the VEGF-receptor 1 was enhanced in PAFs. Receptor inhibition by a specific VEGF-receptor inhibitor or intrinsic down-regulation by RNAi of the VEGF-receptor kinase in 3D gel cultures resulted in significant reduction in collagen degradation associated with increased tissue inhibitor of metalloproteinase expression, suggesting that Flt-1 may contribute to periodontitis. ConclusionBased on the finding that VEGF-receptor kinase inhibition impaired collagen degradation pathways, Flt-1 may represent a candidate for therapeutic approaches in periodontitis.
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| 8. |
- Ohshima, Mitsuhiro, et al.
(författare)
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In vitro characterization of the cytokine profile of the epithelial cell rests of Malassez
- 2008
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Ingår i: Journal of Periodontology. - : Wiley. - 0022-3492 .- 1943-3670. ; 79:5, s. 912-919
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Tidskriftsartikel (refereegranskat)abstract
- BACKGROUND: The epithelial cell rests of Malassez (ERM) are an integral part of the periodontal ligament and are considered to play an important role in dental pathology. Surprisingly, this cell type is poorly described and is often disregarded in the context of periodontal research. The aim of this study was to establish primary cell cultures of human ERM, characterize the cytokine profile, and compare it to other periodontal cell entities. METHODS: ERM-derived epithelial cells were isolated from the periodontal ligament of three subjects. A cytokine antibody array, including 120 cytokines in two membranes, was used to determine the cytokine profile of conditioned medium from the ERM-derived epithelial cells. The results were compared to those of gingival epithelial cells and periodontal ligament fibroblasts. RESULTS: ERM-derived epithelial cells expressed 29 of 120 cytokines in significant amounts, including cytokines, chemokines, growth factors, and related proteins, such as interleukin (IL)-1, -6, -8, and -10; granulocyte macrophage-colony stimulating factor; monocyte chemoattractant protein (MCP)-1, -2, and -3; amphiregulin; glial-derived neurotrophic factor; vascular endothelial growth factor; and insulin-like growth factor binding protein-2. The cytokine profile of ERM cells was similar to that of gingival epithelial cells but strikingly different from the profile of periodontal ligament fibroblasts. CONCLUSIONS: The results indicated that, via paracrine secretion of a variety of soluble factors, the ERM cells actively take part in the homeostasis of the periodontium. Therefore, future research on the pathophysiology of periodontal tissue should include this often overlooked cell type.
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| 9. |
- Yamaguchi, Yoko, et al.
(författare)
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Targeting hepatocyte growth factor in epithelial-stromal interactions in an in vitro experimental model of human periodontitis
- 2021
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Ingår i: Odontology. - : Springer Nature. - 1618-1247 .- 1618-1255. ; 109:4, s. 912-920
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Tidskriftsartikel (refereegranskat)abstract
- Periodontitis is a chronic inflammatory disease leading to progressive connective tissue degradation and loss of the tooth-supporting bone. Clinical and experimental studies suggest that hepatocyte growth factor (HGF) is involved in the dysregulated fibroblast-epithelial cell interactions in periodontitis. The aim of this study was to explore effects of HGF to impact fibroblast-induced collagen degradation. A patient-derived experimental cell culture model of periodontitis was applied. Primary human epithelial cells and fibroblasts isolated from periodontitis-affected gingiva were co-cultured in a three-dimensional collagen gel. The effects of HGF neutralizing antibody on collagen gel degradation were tested and transcriptome analyses were performed. HGF neutralizing antibody attenuated collagen degradation and elicited expression changes of genes related to extracellular matrix (ECM) and cell adhesion, indicating that HGF signaling inhibition leads to extensive impact on cell-cell and cell-ECM interactions. Our study highlights a potential role of HGF in periodontitis. Antagonizing HGF signaling by a neutralizing antibody may represent a novel approach for periodontitis treatment.
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