1. |
- Beal, Jacob, et al.
(författare)
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Robust estimation of bacterial cell count from optical density
- 2020
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Ingår i: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
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Tidskriftsartikel (refereegranskat)abstract
- Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
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2. |
- Gu, Peng, et al.
(författare)
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A metabolite from commensal Candida albicans enhances the bactericidal activity of macrophages and protects against sepsis
- 2023
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Ingår i: Cellular & Molecular Immunology. - London : Nature Publishing Group. - 1672-7681 .- 2042-0226. ; 20:10, s. 1156-1170
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Tidskriftsartikel (refereegranskat)abstract
- The gut microbiome is recognized as a key modulator of sepsis development. However, the contribution of the gut mycobiome to sepsis development is still not fully understood. Here, we demonstrated that the level of Candida albicans was markedly decreased in patients with bacterial sepsis, and the supernatant of Candida albicans culture significantly decreased the bacterial load and improved sepsis symptoms in both cecum ligation and puncture (CLP)-challenged mice and Escherichia coli-challenged pigs. Integrative metabolomics and the genetic engineering of fungi revealed that Candida albicans-derived phenylpyruvate (PPA) enhanced the bactericidal activity of macrophages and reduced organ damage during sepsis. Mechanistically, PPA directly binds to sirtuin 2 (SIRT2) and increases reactive oxygen species (ROS) production for eventual bacterial clearance. Importantly, PPA enhanced the bacterial clearance capacity of macrophages in sepsis patients and was inversely correlated with the severity of sepsis in patients. Our findings highlight the crucial contribution of commensal fungi to bacterial disease modulation and expand our understanding of the host-mycobiome interaction during sepsis development. © 2023, The Author(s), under exclusive licence to CSI and USTC.
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3. |
- Jianfeng, Lin, 1994, et al.
(författare)
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Intelligent ship anti-rolling control system based on a deep deterministic policy gradient algorithm and the Magnus effect
- 2022
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Ingår i: Physics of Fluids. - : AIP Publishing. - 1070-6631 .- 1089-7666. ; 34:5
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Tidskriftsartikel (refereegranskat)abstract
- Anti-rolling devices are widely used in modern shipboard components. In particular, ship anti-rolling control systems are developed to achieve a wide range of ship speeds and efficient anti-rolling capabilities. However, factors that are challenging to solve accurately, such as strong nonlinearities, a complex working environment, and hydrodynamic system parameters, limit the investigation of the rolling motion of ships at sea. Moreover, current anti-rolling control systems still face several challenges, such as poor nonlinear adaptability and manual parameter adjustment. In this regard, this study developed a dynamic model for a ship anti-rolling system. In addition, based on deep reinforcement learning (DRL), an efficient anti-rolling controller was developed using a deep deterministic policy gradient (DDPG) algorithm. Finally, the developed system was applied to a ship anti-rolling device based on the Magnus effect. The advantages of reinforcement learning adaptive control enable controlling an anti-rolling system under various wave angles, ship speeds, and wavelengths. The results revealed that the anti-rolling efficiency of the intelligent ship anti-rolling control method using the DDPG algorithm surpassed 95% and had fast convergence. This study lays the foundation for developing a DRL anti-rolling controller for full-scale ships.
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