| 1. |
- Gui, Xinru, et al.
(författare)
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Identification and validation of volatile organic compounds in bile for differential diagnosis of perihilar cholangiocarcinoma
- 2023
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Ingår i: Clinica Chimica Acta. - : Elsevier. - 0009-8981 .- 1873-3492. ; 541
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Tidskriftsartikel (refereegranskat)abstract
- Early and differential diagnosis of perihilar cholangiocarcinoma (PHCCA) is highly challenging. This study aimed to evaluate whether volatile organic compounds (VOCs) in bile samples could be emerging diagnostic biomarkers for PHCCA. We collected 200 bile samples from patients with PHCCA and benign biliary diseases (BBD), including a 140-patient training cohort and an 60-patient test cohort. Gas chromatography-ion mobility spec-trometry (GC-IMS) was used for VOCs detection. The predictive models were constructed using machine learning algorithms. Our analysis detected 19 VOC substances using GC-IMS in the bile samples and resulted in the identification of three new VOCs, 2-methoxyfuran, propyl isovalerate, and diethyl malonate that were found in bile. Unsupervised hierarchical clustering analysis supported that VOCs detected in the bile could distinguish PHCCA from BBD. Twelve VOCs defined according to 32 signal peaks had significant statistical significance between BBD and PHCCA, including four up-regulated VOCs in PHCCA, such as 2-ethyl-1-hexanol, propyl iso-valerate, cyclohexanone, and acetophenone, while the rest eight VOCs were down-regulated. ROC curve analysis revealed that machine learning models based on VOCs could help diagnosing PHCCA. Among them, SVM pro-vided the highest AUC of 0.966, with a sensitivity and specificity of 93.1% and 100%, respectively. The diag-nostic model based on different VOC spectra could be a feasible method for the differential diagnosis of PHCCA.
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| 2. |
- Chen, Yulong, et al.
(författare)
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Hypercysteinemia promotes atherosclerosis by reducing protein S-nitrosylation.
- 2015
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Ingår i: Biomedicine and Pharmacotherapy. - : Elsevier BV. - 1950-6007 .- 0753-3322. ; 70, s. 253-259
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Tidskriftsartikel (refereegranskat)abstract
- Protein S-nitrosylation plays important role in the regulation of cardiovascular functions in nitric oxide (NO) Pathway. Hypercysteinemia (HHcy) is an independently risk factor for atherosclerosis. We hypothesized that HHcy promotes atherosclerosis by reducing level of vascular protein S-nitrosylation. The aim of present study is to investigate effect of HHcy on vascular protein S-nitrosylation. A total of 45 male apoE-/- mice were randomly divided into three groups. The control group was fed a Western-type diet. The HHcy group was fed a diet containing 4.4% l-methionine, and the HHcy+NONOate group was fed a diet containing 4.4% l-methionine and administrated NONOate (ip). Human umbilical vein endothelial cells were performed for in vitro experiment. Plasma lipids were measured every 4 weeks. After 12 weeks, aortic atherosclerotic lesion areas were detected as well as cellular components. The levels of plasma homocysteine (Hcy) and NO were measured. S-nitrosylation was detected using immunofluorescence, and further confirmed by biotin switch method. We found that compared with the control group, Hcy levels, and atherosclerotic plaque, and content of vascular smooth muscle cells and macrophages in lesions significantly increased, and levels of NO significantly decreased in the HHcy group. However, NONOate reverses this effect. In addition, Hcy significantly reduced protein S-nitrosylation in human umbilical vein endothelial cells. This reduction of protein S-nitrosylation was accompanied by reduced levels of NO. Our results suggested that Hcy promoted atherosclerosis by inhibiting vascular protein S-nitrosylation.
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| 3. |
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| 4. |
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| 5. |
- Kang, Naixin, et al.
(författare)
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Antischistosomal Properties of Hederacolchiside A1 Isolated from Pulsatilla chinensis
- 2018
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Ingår i: Molecules. - : MDPI. - 1431-5157 .- 1420-3049. ; 23:6
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Tidskriftsartikel (refereegranskat)abstract
- Background: Schistosomiasis is a major neglected disease for which the current control strategy involves mass treatment with praziquantel, the only available drug. Hence, there is an urgent need to develop new antischistosomal compounds.Methods: The antischistosomal activity of hederacolchiside A1 (HSA) were determined by total or female worm burden reductions in mice harboring Schistosoma japonicum or S. mansoni. Pathology parameters were detected on HSA against 1-day-old S. japonicum-harboring mice. Moreover, we confirmed the antischistosomal effect of HSA on newly transformed schistosomula (NTS) of S. japonicum in vitro.Results: HSA, a natural product isolated from Pulsatilla chinensis (Bunge) Regel, was initially corroborated to possess promising antischistosomal properties. We demonstrated that HSA had high activity against S. japonicum and S. mansoni less in 11 days old parasites harbored in mice. The antischistosomal effect was even more than the currently used drugs, praziquantel, and artesunate. Furthermore, HSA could ameliorate the pathology parameters in mice harboring 1-day-old juvenile S. japonicum. We also confirmed that HSA-mediated antischistosomal activity is partly due to the morphological changes in the tegument system when NTS are exposed to HSA.Conclusions: HSA may have great potential to be an antischistosomal agent for further research.
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| 6. |
- Kouveliotis Lysikatos, Iasonas, 1989-, et al.
(författare)
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Towards an Internet-like Power Grid
- 2022
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Ingår i: Journal of Modern Power Systems and Clean Energy. - : Journal of Modern Power Systems and Clean Energy. - 2196-5625 .- 2196-5420. ; 10:1, s. 1-11
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Forskningsöversikt (refereegranskat)abstract
- The great challenges faced by modern power systems require a fresh look at the conventional operation paradigm. The significant challenges faced by modern power systems require an innovative method for the conventional operation paradigm. We claim that the decarbonization of the power grid and extensive electrification of numerous sectors of human activity can only be fostered by a self-adaptable and smart power grid that manifests similar qualities to those of the Internet. The Internet is constructed on a layered architecture that facilitates technology innovations and its intelligence is distributed throughout a hierarchy of networks. In this paper, the fundamental differences between the network data flows and power flows are examined, and the basic requirements for an innovative operation paradigm are highlighted. The current power grid is operated in a highly inflexible, centralized manner to meet increased security goals. A new highly flexible, distributed architecture can be realized by distributing the operation responsibility in smaller areas or even in grid components that can make autonomous decisions. The characteristics of such a power grid are presented, and the key features and advances for the on-going transition to a sustainable power system are identified. Finally, a case study on distributed voltage control is presented and discussed.
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| 7. |
- Li, Pei-Zhou, et al.
(författare)
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A highly porous metal-organic framework for large organic molecule capture and chromatographic separation
- 2017
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Ingår i: Chemical Communications. - : Royal Society of Chemistry (RSC). - 1359-7345 .- 1364-548X. ; 53:24, s. 3434-3437
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Tidskriftsartikel (refereegranskat)abstract
- A highly porous metal-organic framework (MOF) with large pores was successfully obtained via solvothermal assembly of a ''click''-extended tricarboxylate ligand and Zn(II) ions. The inherent feature of large-molecule accessible pores endows the MOF with a unique property for utilization toward large guest molecules.
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| 8. |
- Li, Shilei, 1982, et al.
(författare)
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Evaluation of hardening behaviors in ion-irradiated Fe–9Cr and Fe–20Cr alloys by nanoindentation technique
- 2016
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Ingår i: Journal of Nuclear Materials. - : Elsevier BV. - 0022-3115. ; 478, s. 50-56
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Tidskriftsartikel (refereegranskat)abstract
- The ion irradiation hardening behaviors of Fee9 wt% Cr and Fee20 wt% Cr model alloys were investigatedby nanoindentation technique. The specimens were irradiated with 3 MeV Fe11þ ions at roomtemperature up to 1 and 5 dpa for Fee9Cr alloy and 1 and 2.5 for Fee20Cr alloy. The ratio of averagehardness in the same depth of irradiated and unirradiated (Hirr. av/Hunirr. av) was used to determine thecritical indentation depth hcrit to eliminate the softer substrate effect. The NixeGao model was used toexplain the indentation size effect. Irradiation hardening is clearly observed in both Fee9Cr alloy and Fee20Cr alloy after ion irradiation. The differences of ISE and irradiation hardening behaviors between Fee9Cr and Fee20Cr alloys are considered to be due to their different microstructures and microstructuralevolution under ion irradiation.
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| 9. |
- Liu, Guofeng, et al.
(författare)
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Helicity Inversion of Supramolecular Hydrogels Induced by Achiral Substituents
- 2017
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Ingår i: ACS Nano. - : American Chemical Society (ACS). - 1936-0851 .- 1936-086X. ; 11:12, s. 11880-11889
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Tidskriftsartikel (refereegranskat)abstract
- Probing the supramolecular chirality of assemblies and controlling their handedness are closely related to the origin of chirality at the supramolecular level and the development of smart materials with desired handedness. However, it remains unclear how achiral residues covalently bonded to chiral amino acids can function in the chirality inversion of supramolecular assemblies. Herein, we report macroscopic chirality and dynamic manipulation of chiroptical activity of hydrogels self-assembled from phenylalanine derivatives, together with the inversion of their handedness achieved solely by exchanging achiral substituents between oligo(ethylene glycol) and carboxylic acid groups. This helicity inversion is mainly induced by distinct stacking mode of the self-assembled building blocks, as collectively confirmed by scanning electron microscopy, circular dichroism, crystallography, and molecular dynamics calculations. Through this straightforward approach, we were able to invert the handedness of helical assemblies by merely exchanging achiral substituents at the terminal of chiral gelators. This work not only presents a feasible strategy to achieve the handedness inversion of helical nanostructures for better understanding of chiral self-assembly process in supramolecular chemistry but also facilities the development of smart materials with controllable handedness in materials science.
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| 10. |
- Liu, Xufei, et al.
(författare)
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Bio-inspired polydopamine nanofiltration membranes modulated by spiro-piperazine
- 2023
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Ingår i: Advanced Membranes. - : Elsevier BV. - 2772-8234. ; 3
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Tidskriftsartikel (refereegranskat)abstract
- Polydopamine (PDA) depositions, inspired by mussel foot adhesive proteins, represent a versatile method for preparing separation membranes. However, PDA-based nanofiltration membranes are limited by the long preparation time and moderate flux. This work modulated PDA deposition processes with a spiro-piperazine (SPIP) molecule containing two secondary amine groups and a quaternary ammonium salt. The SPIP could be covalently inserted into PDA coating structures via Michael addition reaction to accelerate the deposition process of PDA and reduce its aggregation. In addition, the rigid and spiro configuration of SPIP molecules provides higher fractional free volume and leads to looser and more uniform structures in the PDA coating. As such, water permeance of PDA/SPIP membranes is 73 L m−2 h−1 bar−1, 4.6 times improved compared with PDA control membranes, while the dye rejection (>99% for Congo red) is maintained high. These results demonstrate that SPIP is an effective molecule for the structure and performance engineering of mussel-inspired PDA nanofiltration membranes.
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| 11. |
- Liu, Yanli, et al.
(författare)
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Towards a limits of acceptability approach to the calibration of hydrological models : Extending observation error
- 2009
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Ingår i: Journal of Hydrology. - : Elsevier BV. - 0022-1694 .- 1879-2707. ; 367:1-2, s. 93-103
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Tidskriftsartikel (refereegranskat)abstract
- Within the GLUE methodology, there are a number of advantages of taking a limits of acceptability approach to model evaluation for non-ideal applications where the strong assumptions of statistical identification might be difficult to justify. However, there is a question of how the limits of acceptability might be specified in a way that reflects the different Sources of uncertainty in the modeling process. Here, a novel method for identifying behavioural models in an extended GLUE methodology is developed and applied to an application of Dynamic TOPMODEL to the Attert catchment in Luxemburg with semi-distributed inputs to nested sub-catchments. The results raise some important issues about testing model structures as hypotheses of catchment responses. (C) 2009 Elsevier B.V. All rights reserved.
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| 12. |
- Ning, Yujie, et al.
(författare)
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Comparative analysis of the gut microbiota composition between knee osteoarthritis and Kashin-Beck disease in Northwest China
- 2022
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Ingår i: Arthritis Research & Therapy. - : BioMed Central. - 1478-6362. ; 24:1
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Tidskriftsartikel (refereegranskat)abstract
- Background: Osteoarthritis (OA) and Kashin-Beck disease (KBD) both are two severe osteochondral disorders. In this study, we aimed to compare the gut microbiota structure between OA and KBD patients.Methods: Fecal samples collected from OA and KBD patients were used to characterize the gut microbiota using 16S rDNA gene sequencing. To identify whether gut microbial changes at the species level are associated with the genes or functions of the gut bacteria between OA and KBD groups, metagenomic sequencing of fecal samples from OA and KBD subjects was performed.Results: The OA group was characterized by elevated Epsilonbacteraeota and Firmicutes levels. A total of 52 genera were identified to be significantly differentially abundant between the two groups. The genera Raoultella, Citrobacter, Flavonifractor, g__Lachnospiraceae_UCG-004, and Burkholderia-Caballeronia-Paraburkholderia were more abundant in the OA group. The KBD group was characterized by higher Prevotella_9, Lactobacillus, Coprococcus_2, Senegalimassilia, and Holdemanella. The metagenomic sequencing showed that the Subdoligranulum_sp._APC924/74, Streptococcus_parasanguinis, and Streptococcus_salivarius were significantly increased in abundance in the OA group compared to those in the KBD group, and the species Prevotella_copri, Prevotella_sp._CAG:386, and Prevotella_stercorea were significantly decreased in abundance in the OA group compared to those in the KBD group by using metagenomic sequencing.Conclusion: Our study provides a comprehensive landscape of the gut microbiota between OA and KBD patients and provides clues for better understanding the mechanisms underlying the pathogenesis of OA and KBD.
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| 13. |
- Ning, Yujie, et al.
(författare)
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Genetic Variants and Protein Alterations of Selenium- and T-2 Toxin-Responsive Genes Are Associated With Chondrocytic Damage in Endemic Osteoarthropathy
- 2022
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Ingår i: Frontiers in Genetics. - : Frontiers Media S.A.. - 1664-8021. ; 12
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Tidskriftsartikel (refereegranskat)abstract
- The mechanism of environmental factors in Kashin-Beck disease (KBD) remains unknown. We aimed to identify single nucleotide polymorphisms (SNPs) and protein alterations of selenium- and T-2 toxin-responsive genes to provide new evidence of chondrocytic damage in KBD. This study sampled the cubital venous blood of 258 subjects including 129 sex-matched KBD patients and 129 healthy controls for SNP detection. We applied an additive model, a dominant model, and a recessive model to identify significant SNPs. We then used the Comparative Toxicogenomics Database (CTD) to select selenium- and T-2 toxin-responsive genes with the candidate SNP loci. Finally, immunohistochemistry was applied to verify the protein expression of candidate genes in knee cartilage obtained from 15 subjects including 5 KBD, 5 osteoarthritis (OA), and 5 healthy controls. Forty-nine SNPs were genotyped in the current study. The C allele of rs6494629 was less frequent in KBD than in the controls (OR = 0.63, p = 0.011). Based on the CTD database, PPARG, ADAM12, IL6, SMAD3, and TIMP2 were identified to interact with selenium, sodium selenite, and T-2 toxin. KBD was found to be significantly associated with rs12629751 of PPARG (additive model: OR = 0.46, p = 0.012; dominant model: OR = 0.45, p = 0.049; recessive model: OR = 0.18, p = 0.018), rs1871054 of ADAM12 (dominant model: OR = 2.19, p = 0.022), rs1800796 of IL6 (dominant model: OR = 0.30, p = 0.003), rs6494629 of SMAD3 (additive model: OR = 0.65, p = 0.019; dominant model: OR = 0.52, p = 0.012), and rs4789936 of TIMP2 (recessive model: OR = 5.90, p = 0.024). Immunohistochemistry verified significantly upregulated PPARG, ADAM12, SMAD3, and TIMP2 in KBD compared with OA and normal controls (p < 0.05). Genetic polymorphisms of PPARG, ADAM12, SMAD3, and TIMP2 may contribute to the risk of KBD. These genes could promote the pathogenesis of KBD by disturbing ECM homeostasis.
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| 14. |
- Wu, Hongwei, et al.
(författare)
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Crystal Multi-Conformational Control Through Deformable Carbon-Sulfur Bond for Singlet-Triplet Emissive Tuning
- 2019
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Ingår i: Angewandte Chemie International Edition. - : WILEY-V C H VERLAG GMBH. - 1433-7851 .- 1521-3773. ; 58:13, s. 4328-4333
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Tidskriftsartikel (refereegranskat)abstract
- Crystal-state luminophores have been of great interest in optoelectronics for years, whereas the excited state regulation at the crystal level is still restricted by the lack of control ways. We report that the singlet-triplet emissive property can be profoundly regulated by crystal conformational distortions. Employing fluoro-substituted tetrakis(arylthio)benzene luminophores as prototype, we found that couples of molecular conformations formed during different crystallizations. The deformable carbon-sulphur bond essentially drove the distortion of the molecular conformation and varied the stacking mode, together with diverse non-covalent interactions, leading to the proportional adjustment of the fluorescence and phosphorescence bands. This intrinsic strategy was further applied for solid-state multicolor emissive conversion and mechanoluminescence, probably offering new insights for design of smart crystal luminescent materials.
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| 15. |
- Xu, Li, 1986, et al.
(författare)
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Graphene-based biosensors for the detection of prostate cancer protein biomarkers: a review
- 2019
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Ingår i: BMC Chemistry. - : Springer Science and Business Media LLC. - 2661-801X. ; 13:1
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Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)abstract
- Prostate cancer (PC) is the sixth most common cancer type in the world, which causes approximately 10% of total cancer fatalities. The detection of protein biomarkers in body fluids is the key topic for the diagnosis and prognosis of PC. Highly sensitive screening of PC is the most effective approach for reducing mortality. Thus, there are a growing number of literature that recognizes the importance of new technologies for early diagnosis of PC. Graphene is playing an important role in the biosensor field with remarkable physical, optical, electrochemical and magnetic properties. Many recent studies demonstrated the potential of graphene materials for sensitive detection of protein biomarkers. In this review, the graphene-based biosensors toward PC analysis are mainly discussed in two groups: Firstly, novel biosensor interfaces were constructed through the modification of graphene materials onto sensor surfaces. Secondly, ingenious signal amplification strategies were developed using graphene materials as catalysts or carriers. Graphene-based biosensors have exhibited remarkable performance with high sensitivities, wide detection ranges, and long-term stabilities.
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| 16. |
- Zhang, Heng, et al.
(författare)
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An improved similarity based adaptive step size glowworm algorithm
- 2015
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Ingår i: Journal of Internet Technology. - 1607-9264 .- 2079-4029. ; 16:5, s. 905-914
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Tidskriftsartikel (refereegranskat)abstract
- This paper presents the similarity based adaptive step size glowworm swarm optimization algorithm (SBASS-GSO), an improved version of glowworm swarm optimization algorithm (GSO). The standard GSO algorithm lacks unified metric standard to different problems in the selection of neighbor set, which makes the algorithm converge slowly because of improper selection. Because the step size s is fixed, the oscillation phenomenon may occur in local search space, which leads to inferior search accuracy In SBASS-GSO algorithm, we change neighborhood definition base on the similarity not on the distance. The neighborhood is selected by computing average similarity, which provides priori knowledge for the adaptive size s. The dynamic size s is useful for removing oscillation phenomenon and improving the convergence speed. Experimental results demonstrate the efficacy of the proposed glowworm algorithm in capturing multiple optima of a series of complex test functions, such as Zakharov and Sphere functions. We also provide some comparisons of SBASS-GSO with GSO and verify the superiority in the precision and convergence speed.
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| 17. |
- Zhao, Dandan, et al.
(författare)
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Investigation of ion irradiation hardening behaviors of tempered and long-term thermal aged T92 steel
- 2018
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Ingår i: Journal of Nuclear Materials. - : Elsevier BV. - 0022-3115. ; 511, s. 191-199
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Tidskriftsartikel (refereegranskat)abstract
- 9Cr ferritic/martensitic steels are promising materials for in-core components in advanced Gen-IV reactors. In these applications, their long-term microstructural stability under thermal exposure and resistance to neutron irradiation are essential. Tempered (unaged) and long-term thermal aged T92 samples were used to evaluate the effects of thermal aging and ion irradiation on the microstructure and micromechanical properties of the steel. Both the tempered and aged samples were irradiated with 3 MeV Fe11+ions to 0.25, 0.50, 1.00 and 5.00 dpa at room temperature. Using the nanoindentation technique, the irradiation hardening behaviors of T92 steel were investigated. The irradiation hardening effect was observed in both the tempered and aged T92 samples. To eliminate the soft substrate effect, the critical indentation depth was determined using the ratio of the average hardness of irradiated and unirradiated samples at the same depth. Under the same irradiation conditions, the macroscopic hardness values of the aged T92 samples after irradiation were lower than those of the tempered samples. The irradiation hardening effect was more significant in the aged T92 due to the decreased dislocation density and the coarsened martensitic lath after long-term thermal aging.
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| 18. |
- Zhao, Dandan, et al.
(författare)
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Proton irradiation induced defects in T92 steels: An investigation by TEM and positron annihilation spectroscopy
- 2019
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Ingår i: Nuclear Instruments and Methods in Physics Research, Section B: Beam Interactions with Materials and Atoms. - : Elsevier BV. - 0168-583X. ; 442, s. 59-66
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Tidskriftsartikel (refereegranskat)abstract
- In order to investigate proton irradiation damage on ferritic/martensitic T92 steels, both the unaged and aged (650 °C for 15,000 h) T92 steels were irradiated with 250 keV protons to 0.01, 0.05 and 0.20 dpa at room temperature due to the lower dose rate of protons compared with heavy-ions. The microstructural evolution induced by thermal aging and proton irradiation was studied by transmission electron microscopy and positron annihilation spectroscopy, and the corresponding micromechanical property changes were investigated by nano-indentation. After 0.20 dpa proton irradiation, the dominant irradiation-induced dislocation loops were a0100 type loops for both the unaged and aged samples. The dislocation-type defects in the aged T92 sample were larger in size and higher in number density, compared with those in the unaged samples. Less vacancy-type defects induced by protons were detected in the aged than the unaged T92 samples under the same irradiation conditions. The higher number density of dislocation-type defects led to more severe irradiation hardening in the aged T92 samples.
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